SitesBLAST

P97084 Threonine-phosphate decarboxylase; L-threonine-O-3-phosphate decarboxylase; EC 4.1.1.81 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
30% identity, 89% coverage: 25:352/369 of query aligns to 27:355/364 of P97084

query
sites
P97084

L

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N32 (= N30) binding O-phospho-L-threonine
N157 (= N153) binding O-phospho-L-threonine
K216 (= K212) modified: N6-(pyridoxal phosphate)lysine
R323 (= R320) binding O-phospho-L-threonine
R337 (= R334) binding O-phospho-L-threonine

Sites not aligning to the query:

8:9 binding O-phospho-L-threonine

1lc8A Crystal structure of l-threonine-o-3-phosphate decarboxylase from s. Enterica complexed with its reaction intermediate (see paper)
30% identity, 89% coverage: 25:352/369 of query aligns to 21:349/356 of 1lc8A

query
sites
1lc8A

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binding {3-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-2-methyl-propyl}-phosphonic acid: G78 (= G79), E79 (≠ A80), T80 (≠ I81), F102 (= F103), N151 (= N153), D179 (= D179), A181 (= A181), S207 (= S209), T209 (= T211), K210 (= K212), R218 (= R220), R317 (= R320), R331 (= R334)

Sites not aligning to the query:

binding {3-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-2-methyl-propyl}-phosphonic acid: 2, 3

1geyA Crystal structure of histidinol-phosphate aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate (see paper)
28% identity, 85% coverage: 36:350/369 of query aligns to 24:335/335 of 1geyA

query
sites
1geyA

P

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D

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L

binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: G67 (= G79), A68 (= A80), D69 (≠ I81), Y94 (≠ F103), N141 (= N153), D168 (= D179), A170 (= A181), Y171 (≠ F182), T195 (≠ S209), S197 (≠ T211), K198 (= K212), R206 (= R220), R319 (= R334)

Sites not aligning to the query:

binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: 20

1fg7A Crystal structure of l-histidinol phosphate aminotransferase with pyridoxal-5'-phosphate (see paper)
28% identity, 85% coverage: 36:350/369 of query aligns to 38:349/354 of 1fg7A

query
sites
1fg7A

P

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F

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V

E

A

H

D

V

I

A

A

G

A

Q

Q

L

A

L

L

H

S

-

P

-

Q

-

G

-

I

C

V

G

A

E

M

Y

R

E

E

R

R

E

-

V

V

H

A

A

Q

I

I

V

I

R

A

E

E

G

R

R

E

K

Y

Q

L

I

I

A

A

D

A

G

L

L

K

A

E

G

I

N

P

S

C

V

V

I

E

T

Q

L

V

H

F

G

D

G

S

A

E

A

T

N

N

F

Y

F

I

F

L

A

A

S

R

V

F

A

K

D

-

E

-

F

-

S

A

L

S

D

S

A

A

L

V

F

F

D

K

V

S

L

L

A

W

E

D

R

Q

G

G

I

I

L

I

V

L

R

R

D

D

C

-

R

Q

N

N

F

K

E

Q

G

P

V

S

P

L

P

S

K

G

Y

C

F

L

R

R

F

I

C

T

I

V

R

G

T

T

S

R

D

E

E

E

N

S

R

Q

R

R

L

V

I

I

E

D

A

A

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G81 (= G79), A82 (= A80), D83 (≠ I81), Y108 (≠ F103), N155 (= N153), D182 (= D179), A184 (= A181), Y185 (≠ F182), T209 (≠ S209), S211 (≠ T211), K212 (= K212), R220 (= R220)

1fg3A Crystal structure of l-histidinol phosphate aminotransferase complexed with l-histidinol (see paper)
28% identity, 85% coverage: 36:350/369 of query aligns to 38:349/354 of 1fg3A

query
sites
1fg3A

P

P

P

T

L

A

Q

V

E

E

L

F

F

Q

S

L

T

T

S

Q

I

Q

P

T

L

L

A

N

P

R

Y

Y

P

P

S

E

M

C

D

Q

G

P

H

K

G

A

V

V

R

I

E

E

F

N

Y

Y

A

A

A

Q

R

Y

F

A

G

G

L

V

D

K

P

P

V

E

C

Q

V

V

L

L

S

V

T

S

N

R

G
|
G

A
|
A

I
x
D

E

E

G

G

L

I

Y

E

L

L

I

L

P

I

R

R

A

A

L

F

-

C

-

E

-

P

G

G

I

K

K

D

R

A

V

I

L

L

V

Y

P

C

Q

P

P

P

S

T

F
x
Y

F

G

D

M

Y

Y

G

S

R

V

A

S

C

A

R

E

L

T

A

I

G

G

A

V

E

E

V

C

V

R

P

T

L

V

A

P

L

T

S

L

E

D

S

N

G

W

S

Q

F

L

A

D

F

L

P

Q

G

G

I

I

D

-

K

-

L

-

A

S

D

D

A

K

L

L

A

D

G

G

C

V

D

K

A

V

L

V

L

Y

A

V

G

C

S

S

P

P

N

N

N
|
N

P

P

T

T

G

G

T

Q

L

L

I

I

-

N

P

P

K

Q

E

D

L

F

L

R

L

T

A

L

L

L

A

E

C

L

R

T

F

R

P

G

E

K

K

A

R

I

F

V

I

V

V

A

D
|
D

E

E

A
|
A

F
x
Y

I

I

Q

E

F

F

T

C

E

-

A

-

F

-

P

P

S

Q

N

A

S

S

L

L

M

A

T

G

E

W

I

L

K

A

A

E

F

Y

R

P

N

H

I

L

A

A

V

I

V

L

H

R

S
x
T

L

L

T
x
S

K
|
K

F

A

Y

F

A

A

I

L

A

A

G

G

L

L

R
|
R

L

C

G

G

A

F

V

T

I

L

A

A

H

N

P

E

S

E

M

V

I

I

R

N

Q

L

L

L

Y

M

G

K

Y

V

K

I

E

A

P

P

W

Y

T

P

V

L

N

S

A

T

V

P

A

V

E

A

H

D

V

I

A

A

G

A

Q

Q

L

A

L

L

H

S

-

P

-

Q

-

G

-

I

C

V

G

A

E

M

Y

R

E

E

R

R

E

-

V

V

H

A

A

Q

I

I

V

I

R

A

E

E

G

R

R

E

K

Y

Q

L

I

I

A

A

D

A

G

L

L

K

A

E

G

I

N

P

S

C

V

V

I

E

T

Q

L

V

H

F

G

D

G

S

A

E

A

T

N

N

F

Y

F

I

F

L

A

A

S

R

V

F

A

K

D

-

E

-

F

-

S

A

L

S

D

S

A

A

L

V

F

F

D

K

V

S

L

L

A

W

E

D

R

Q

G

G

I

I

L

I

V

L

R
|
R

D

D

C

-

R

Q

N

N

F

K

E

Q

G

P

V

S

P

L

P

S

K

G

Y

C

F

L

R
|
R

F

I

C

T

I

V

R

G

T

T

S

R

D

E

E

E

N

S

R

Q

R

R

L

V

I

I

E

D

A

A

L

L

binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: D83 (≠ I81), Y108 (≠ F103), N155 (= N153), K212 (= K212), R320 (= R320), R333 (= R334)
binding pyridoxal-5'-phosphate: G81 (= G79), A82 (= A80), D83 (≠ I81), Y108 (≠ F103), N155 (= N153), D182 (= D179), A184 (= A181), Y185 (≠ F182), T209 (≠ S209), S211 (≠ T211), K212 (= K212), R220 (= R220)

Sites not aligning to the query:

binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: 18, 34

7szpA Crystal structure of histidinol-phosphate aminotransferase from klebsiella pneumoniae subsp. Pneumoniae (strain hs11286)
28% identity, 83% coverage: 44:351/369 of query aligns to 46:350/353 of 7szpA

query
sites
7szpA

T

T

S

E

I

Q

P

T

L

L

A

N

P

R

Y

Y

P

P

S

E

M

P

D

Q

G

P

H

K

G

A

V

V

R

I

E

E

F

S

Y

Y

A

A

A

R

R

Y

F

A

G

E

L

V

D

K

P

P

V

E

C

Q

V

V

L

L

S

V

T

S

N

R

G
|
G

A
|
A

I
x
D

E

E

G

G

L

I

Y

E

L

L

I

L

P

I

R

R

A

A

L

F

-

C

-

E

-

P

G

G

I

E

K

D

R

A

V

V

L

L

V

Y

P

C

Q

P

P

P

S

T

F
x
Y

F

G

D

M

Y

Y

G

S

R

V

A

S

C

A

R

E

L

T

A

I

G

G

A

V

E

E

V

C

V

R

P

T

L

V

A

P

L

T

S

L

E

A

S

D

G

W

S

Q

F

L

A

D

F

L

P

P

G

G

I

I

D

E

K

A

L

R

A

L

D

D

A

-

L

-

A

-

G

G

C

V

D

K

A

V

L

V

L

F

A

V

G

C

S

S

P

P

N

N

P

P

T

T

G

G

T

Q

L

I

I

I

-

D

P

P

K

Q

E

S

L

M

L

R

L

D

A

L

L

L

A

E

C

M

R

T

F

R

P

G

E

K

K

A

R

I

F

V

I

V

V

A

D
|
D

E

E

A
|
A

F
x
Y

I

I

Q

E

F

F

T

C

E

-

A

-

F

-

P

P

S

Q

N

A

S

T

L

L

M

A

T

G

E

W

I

L

K

S

A

D

F

Y

R

P

N

H

I

L

A

V

V

V

V

L

H

R

S
x
T

L

L

T
x
S

K
|
K

F

A

Y

F

A

A

I

L

A

A

G

G

L

L

R
|
R

L

C

G

G

A

F

V

T

I

L

A

A

H

N

P

A

S

E

M

V

I

I

R

N

Q

V

L

L

Y

L

G

K

Y

V

K

I

E

A

P

P

W

Y

T

P

V

L

N

S

A

T

V

P

A

V

E

A

H

D

V

I

A

A

G

A

Q

Q

L

A

L

L

H

S

-

P

-

E

-

G

C

I

G

A

E

A

Y

M

E

R

R

Q

E

R

V

V

H

A

A

Q

I

I

V

L

R

D

E

E

G

-

R

R

K

R

Q

Y

I

L

A

V

D

E

G

Q

L

L

A

R

G

G

N

I

S

A

V

C

I

V

-

E

T

Q

L

V

H

F

G

D

G

S

A

E

A

T

N

N

F

Y

F

V

F

L

A

A

S

R

V

I

A

T

D

-

E

-

F

-

S

A

L

S

D

S

A

A

L

V

F

F

D

K

V

S

L

L

A

W

E

D

R

Q

G

G

I

I

L

I

V

L

R

R

D

D

C

-

R

Q

N

N

F

K

E

Q

G

P

V

S

P

L

P

S

K

G

Y

C

F

L

R

R

F

I

C

T

I

I

R

G

T

T

S

R

D

A

E

E

N

S

R

Q

R

R

L

V

I

I

E

D

A

A

L

L

N

T

binding pyridoxal-5'-phosphate: G81 (= G79), A82 (= A80), D83 (≠ I81), Y108 (≠ F103), D182 (= D179), A184 (= A181), Y185 (≠ F182), T209 (≠ S209), S211 (≠ T211), K212 (= K212), R220 (= R220)

P0DV65 L-serine phosphate decarboxylase; CobD homolog SMUL_1544; SmCobD; L-serine O-phosphate decarboxylase; L-Ser-P decarboxylase; Norcobamide biosynthesis protein SMUL_1544; Threonine phosphate decarboxylase-like enzyme; EC 4.1.1.- from Sulfurospirillum multivorans (strain DM 12446 / JCM 15788 / NBRC 109480) (see paper)
26% identity, 92% coverage: 20:358/369 of query aligns to 54:392/392 of P0DV65

query
sites
P0DV65

A

A

S

S

A

E

I

L

S

V

L

L

D

D

F

Y

S

-

V

-

N

-

L

-

A

-

P

-

I

I

E

D

P

R

P

E

L

L

Q

I

E

Q

L

T

F

N

S

A

T

Y

S

K

I

K

P

V

L

L

A

T

P

H

Y

Y

P

P

S

S

M

Q

D

Q

G

-

H

R

G

S

V

L

R

Q

E

K

F

V

Y

M

A

A

A

E

R

S

F

L

G

H

L

V

D

K

P

P

V

E

C

N

V

I

L

F

S

I

T

G

N

N

G

G

A

A

I

T

E

E

G

I

L

I

Y

Q

L

M

I

L

P

L

R

Q

A

Q

L

E

G

E

I

V

K

Q

R

K

V

V

L

A

V

L

P

M

Q

I

P

P

S

T

F

F

F

S

D

S

Y

Y

-

Y

-

E

-

F

-

V

G

G

R

K

A

G

C

C

R

-

L

-

A

-

G

-

A

-

E

E

V

V

P

Y

L

F

A

P

L

L

S

N

E

E

S

R

G

D

S

D

F

Y

A

S

F

F

P

D

G

A

I

-

D

D

K

K

L

Y

A

C

D

Q

A

F

L

I

A

E

G

N

C

E

-

Q

-

P

D

D

A

T

L

V

A

L

G

I

S

N

P

P

N

N

P

P

T

N

G

G

T

A

L

Y

I

L

P

S

K

L

E

E

L

K

L

M

L

H

A

I

L

L

A

L

C

K

R

R

F

L

P

A

-

F

E

V

K

P

R

R

F

I

I

I

V

I

D

D

E

E

A

S

F

F

I

I

Q

H

F

F

T

A

-

Y

-

E

-

D

E

E

A

A

F

L

P

T

S

C

N

L

S

S

L

S

M

T

T

V

E

L

I

F

K

D

A

M

F

Y

R

P

N

N

I

V

A

I

V

I

V

V

H

K

S

S

L

L

T
x
S

K

K

F

D

Y

F

A

G

I

I

A

A

G

G

L

V

R

R

L

L

G

G

A

Y

V

A

I

L

A

M

H

D

P

S

S

R

M

K

I

I

R

D

Q

A

L

L

Y

L

G

E

Y

H

K

G

E

F

P

L

W

W

T

N

V

I

N

N

A

G

V

I

A

G

E

E

H

Y

V

C

A

L

G

R

Q

L

L

F

L

V

H

R

-

E

-

D

-

F

C

L

G

K

E

R

Y

Y

E

E

R

E

E

A

V

R

H

K

A

Q

I

Y

V

I

R

K

E

E

G

M

R

C

K

R

Q

-

I

F

A

K

D

E

G

A

L

L

A

L

G

G

N

I

S

E

V

N

I

V

T

Y

L

V

H

Y

G

P

G

S

A

M

A

A

N

N

F

F

F

V

F

M

-

L

-

K

-

L

-

P

A

S

S

R

V

I

A

K

D

A

E

S

F

F

S

V

L

I

D

S

A

A

L

L

F

-

D

-

V

-

L

L

A

V

E

E

R

Y

G

G

I

I

L

Y

V

V

R

R

D

T

C

M

R

A

N

D

F

K

E

I

G

G

V

V

P

E

P

G

K

E

Y

C

F

I

R

R

F

I

C

A

I

G

R

R

T

T

S

R

D

E

E

E

N

N

R

N

R

C

L

I

I

V

E

M

A

A

L

L

N

K

D

S

F

I

A

L

E

K

L

D

S

S

R

K

S242 (≠ T211) mutation to T: Increased enzyme activity with of L-threonine phosphate and a decreased activity with L-serine phosphate. Exhibits similar activity rates with both substrates.

Sites not aligning to the query:

2:6 mutation Missing: No effect on enzyme activity with L-serine phosphate or with L-threonine phosphate.
2:11 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:16 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:21 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:29 mutation Missing: Loss of enzyme activity with L-serine phosphate and with L-threonine phosphate. No effect in pyridoxal phosphate (PLP)-binding.
22:29 Required for catalytic activity
26 H→A: Significantly decreased enzyme activity. No effect in pyridoxal phosphate (PLP)-binding.

8bj3A Crystal structure of medicago truncatula histidinol-phosphate aminotransferase (hisn6) in complex with histidinol-phosphate (see paper)
28% identity, 85% coverage: 14:326/369 of query aligns to 26:334/360 of 8bj3A

query
sites
8bj3A

R

R

S

L

G

G

D

R

R

K

L

P

A

E

S

D

A

I

I

V

S

K

L

L

D

D

F

A

S

N

V

E

N

N

L

-

A

-

P

P

I

Y

E

G

P

P

-

P

-

E

-

V

L

M

Q

E

E

A

L

L

F

G

S

S

T

I

S

R

I

F

P

P

L

Y

A

V

P

-

Y
|
Y

P

P

S

D

M

P

D

E

G

S

H

R

G

R

V

L

R

R

E

A

F

A

Y

L

A

A

A

Q

R

D

F

S

G

G

L

L

D

E

P

S

V

E

C

Y

V

I

L

L

S

V

T

G

N

C

G
|
G

A
|
A

I
x
D

E

E

G

L

L

I

Y

D

L

L

I

I

P

M

R

R

A

C

L

V

-

L

-

D

-

P

G

G

I

D

K

K

R

I

V

V

L

D

V

C

P

P

Q

-

P

P

S

T

F
|
F

F

T

D
x
M

Y

Y

G

E

R

F

A

D

C

A

R

A

L

V

A

N

G

G

A

A

E

L

V

V

V

I

P

K

L

V

A

P

L

R

S

R

E

P

S

D

G

F

S

S

F

L

A

N

F

V

P

E

G

Q

I

I

D

I

K

E

L

V

A

V

D

K

A

Q

L

-

A

E

G

K

C

P

D

K

A

C

L

I

L

F

A

L

G

T

S

S

P

P

N

N

N
|
N

P

P

T

D

G

G

T

S

L

I

I

I

P

D

K

D

E

D

L

D

L

L

A

K

L

I

A

-

C

L

R

E

F

L

P

P

E

-

K

I

R

L

F

V

I

V

V

L

D
|
D

E

E

A
|
A

F
x
Y

I

I

Q

E

F

F

T

S

E

T

A

I

F

-

P

-

S

-

N

E

S

S

L

K

M

M

T

S

E

W

I

V

K

K

A

K

F

H

R

D

N

N

I

L

A

I

V

V

V

L

H

R

S
x
T

L

F

T
x
S

K
|
K

F

R

Y

A

A

G

I

L

A

A

G

G

L

L

R
|
R

L

V

G

G

A

Y

V

G

I

A

A

F

H

P

P

L

S

S

M

I

I

I

R

K

Q

Y

L

L

Y

W

G

R

Y

A

K

K

E

Q

P

P

W
x
Y

T

N

V

V

N

S

A

V

V

A

A

A

E

E

H

I

V

S

A

A

G

C

Q

A

L

A

L

L

H

Q

C

N

G

P

E

T

Y

Y

E

L

R

E

E

N

V

V

H

K

A

D

I

A

V

L

R

V

E

K

G

E

R

R

K

G

Q

R

I

L

A

F

D

D

G

L

L

L

A

K

G

A

N

V

S

P

V

F

I

L

T

K

L

P

H

F

G

P

G

S

A

H

A

S

N

N

F

F

F

I

F

L

A

C

S

E

V

V

A

T

D

S

E

G

F

V

S

D

L

P

D

K

A

K

L

L

F

K

D

E

V

D

L

L

A

A

E

E

R

M

G

G

I

V

L

M

V

I

R
|
R

D

H

C

Y

R

S

N

N

F

K

E

E

binding [(2~{S})-3-(1~{H}-imidazol-4-yl)-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]propyl] dihydrogen phosphate: Y63 (= Y51), G91 (= G79), A92 (= A80), D93 (≠ I81), F117 (= F103), M119 (≠ D105), N166 (= N153), D190 (= D179), A192 (= A181), Y193 (≠ F182), T217 (≠ S209), S219 (≠ T211), K220 (= K212), R228 (= R220), Y249 (≠ W241), R328 (= R320)

Sites not aligning to the query:

binding [(2~{S})-3-(1~{H}-imidazol-4-yl)-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]propyl] dihydrogen phosphate: 14, 340

3ly1D Crystal structure of putative histidinol-phosphate aminotransferase (yp_050345.1) from erwinia carotovora atroseptica scri1043 at 1.80 a resolution
28% identity, 47% coverage: 64:237/369 of query aligns to 61:238/354 of 3ly1D

query
sites
3ly1D

A

A

R

H

F

H

G

Q

L

V

D

E

P

A

V

P

C

S

V

I

L

L

S

L

T

T

N

A

G
|
G

A
x
S

I
x
S

E

E

G

G

L

I

Y

R

L

A

I

A

P

I

R

E

A

A

L

Y

G

A

I

S

-

L

-

E

K

A

R

Q

V

L

L

V

V

I

P

P

Q

E

P

L

S

T

F
x
Y

F

G

D

D

Y

G

G

E

R

H

A

F

C

A

R

K

L

I

A

A

G

G

A

M

E

K

V

V

V

T

P

K

L

V

A

K

L

M

S

L

E

D

S

N

G

W

S

A

F

F

A

D

F

I

P

E

G

G

I

L

D

K

K

A

L

A

A

V

D

A

A

A

L

Y

A

S

G

G

C

P

D

S

A

I

L

V

L

Y

A

L

G

V

S

N

P

P

N

N

P

P

T

T

G

G

T

T

L

I

I

T

P

P

K

A

E

D

L

V

L

I

L

E

A

P

-

W

L

I

A

A

C

S

R

K

F

P

P

A

E

N

K

T

R

M

F

F

I

I

V

V

D
|
D

E

E

A

A

F
x
Y

I

A

Q

E

F

F

T

V

E

N

-

D

-

P

A

R

F

F

P

R

S

S

N

I

S

S

L

P

M

M

T

I

E

-

I

T

K

Q

A

G

F

A

R

E

N

N

I

I

A

I

V

L

H

K

S
x
T

L

F

T
x
S

K
|
K

F

I

Y

H

A

A

I

M

A

A

G

G

L

M

R
|
R

L

V

G

G

A

Y

V

A

I

V

A

A

H

H

P

P

S

T

M

V

I

I

R

A

Q

L

L

M

Y

G

G

R

Y

Y

binding pyridoxal-5'-phosphate: G76 (= G79), S77 (≠ A80), S78 (≠ I81), Y102 (≠ F103), D179 (= D179), Y182 (≠ F182), T210 (≠ S209), S212 (≠ T211), K213 (= K212), R221 (= R220)

Q0ZQ41 CMP-5'-(3-aminopropyl)phosphonate synthase; EC 2.7.7.-; EC 4.1.1.- from Streptomyces rubellomurinus (strain ATCC 31215) (see paper)
31% identity, 50% coverage: 71:256/369 of query aligns to 329:511/628 of Q0ZQ41

query
sites
Q0ZQ41

P

P

V

E

C

E

V

V

L

V

S

L

T

A

N

N

G

G

A

S

I

S

E

E

G

I

L

I

Y

K

L

I

I

L

P

A

R

R

A

L

L

R

G

G

I

-

K

-

R

N

V

W

L

T

V

V

P

P

Q

V

P

P

S

G

F

F

F

N

D

E

Y

Y

G

-

R

-

A

-

C

E

R

N

L

V

A

V

G

G

A

A

E

E

V

R

V

V

P

H

L

R

A

Y

L

Q

S

L

E

D

S

A

G

P

S

D

F

F

A

R

F

L

P

P

G

V

I

E

D

D

K

Y

L

A

A

A

D

F

A

V

L

R

-

R

A

S

G

G

C

V

D

D

A

T

L

A

L

V

A

V

G

V

S

S

P

P

N

N

P

P

T

T

G

S

T

V

L

G

I

V

P

P

K

L

E

A

L

D

L

L

L

R

A

R

L

L

A

A

C

D

R

L

F

V

-

G

P

P

E

D

K

V

R

L

F

L

I

V

V

I

D

D

E

E

A

S

F

F

I

V

Q

D

F

F

T

A

E

P

A

A

F

-

P

P

S

I

N

A

S

S

L

I

M

G

T

P

E

F

I

L

K

D

A

R

F

H

R

R

N

N

I

V

A

L

V

L

H

K

S

S

L

I

T

S

K
|
K

F

V

Y

Y

A

G

I

V

A

G

G

G

L

I

R

R

L

L

G

G

-

Y

A

A

V

A

I

T

A

A

H

D

P

T

S

E

M

L

I

A

R

R

Q

T

L

L

Y

R

G

A

Y

E

K

L

E

P

P

I

W

W

T

D

V

I

N

N

A

G

V

F

A

A

E

E

H

E

V

F

A

L

G

R

Q

V

L

L

L

P

H

H

K466 (= K212) mutation to A: Lack of decarboxylase activity, produces only CMP-5'-2APn.

Sites not aligning to the query:

37 K→A: Strong decrease in nucleotide transferase activity.

5yhvB Crystal structure of an aminotransferase from mycobacterium tuberculosis
31% identity, 46% coverage: 63:233/369 of query aligns to 80:253/387 of 5yhvB

query
sites
5yhvB

Y
|
Y

A

Q

A

R

R

R

F
x
H

G

G

L

I

-

T

-

V

D

E

P

P

V

D

C

A

V

V

L

V

S

I

T

T

N

T

G
|
G

A
x
S

I
x
S

E

G

G

G

L

F

Y

L

L

L

I

A

P

F

R

L

A

A

L

C

-

F

-

D

G

A

I

G

K

D

R

R

V

V

L

A

V

M

P

A

Q

S

P

P

S

G

F
x
Y

F

P

D

C

Y

Y

G

R

R

N

A

I

C

L

R

S

L

A

A

L

G

G

A

C

E

E

V

V

P

E

L

I

A

P

L

C

S

G

E

P

S

Q

G

T

S

R

F

F

A

Q

F

-

P

P

G

T

I

A

D

Q

K

M

L

L

A

A

D

E

A

I

L

D

A

P

G

P

C

L

D

R

A

G

L

V

A

V

G

A

S

S

P

P

N

A

N
|
N

P

P

T

T

G

G

T

T

L

V

I

I

P

P

K

P

E

E

L

E

L

L

L

A

A

A

L

I

A

A

-

S

-

W

C

C

R

D

F

A

P

S

E

D

K

V

R

R

F

L

I

I

V

S

D
|
D

E

E

A
x
V

F
x
Y

I
x
H

Q

G

F

L

T

V

-

Y

E

Q

A

G

F

A

P

P

S

Q

N
x
T

S

S

L

C

M

A

T

W

E

Q

I

T

K

S

A

-

F

-

R

R

N

N

I

A

A

V

V

V

H
x
N

S
|
S

L

F

T
x
S

K
|
K

F

Y

Y

Y

A

A

I

M

A

T

G

G

L

W

R
|
R

L

L

G

G

A

W

V

L

I

L

A

V

H

-

P

P

S

T

M

V

I

L

R

R

Q

R

binding 2-oxoglutaric acid: Y80 (= Y63), H84 (≠ F67), H205 (≠ I183), T215 (≠ N192), N229 (≠ H208)
binding pyridoxal-5'-phosphate: G98 (= G79), S99 (≠ A80), S100 (≠ I81), Y124 (≠ F103), N173 (= N153), D201 (= D179), V203 (≠ A181), Y204 (≠ F182), S230 (= S209), S232 (≠ T211), K233 (= K212), R241 (= R220)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 63, 264

5yhvA Crystal structure of an aminotransferase from mycobacterium tuberculosis
31% identity, 46% coverage: 63:233/369 of query aligns to 87:260/394 of 5yhvA

query
sites
5yhvA

Y

Y

A

Q

A

R

R

R

F
x
H

G

G

L

I

-

T

-

V

D

E

P

P

V

D

C

A

V

V

L

V

S

I

T

T

N

T

G
|
G

A
x
S

I
x
S

E

G

G

G

L

F

Y

L

L

L

I

A

P

F

R

L

A

A

L

C

-

F

-

D

G

A

I

G

K

D

R

R

V

V

L

A

V

M

P

A

Q

S

P

P

S

G

F
x
Y

F

P

D

C

Y

Y

G

R

R

N

A

I

C

L

R

S

L

A

A

L

G

G

A

C

E

E

V

V

P

E

L

I

A

P

L

C

S

G

E

P

S

Q

G

T

S

R

F

F

A

Q

F

-

P

P

G

T

I

A

D

Q

K

M

L

L

A

A

D

E

A

I

L

D

A

P

G

P

C

L

D

R

A

G

L

V

A

V

G

A

S

S

P

P

N

A

N
|
N

P

P

T

T

G

G

T

T

L

V

I

I

P

P

K

P

E

E

L

E

L

L

L

A

A

A

L

I

A

A

-

S

-

W

C

C

R

D

F

A

P

S

E

D

K

V

R

R

F

L

I

I

V

S

D

D

E

E

A

V

F
x
Y

I
x
H

Q

G

F

L

T

V

-

Y

E

Q

A

G

F

A

P

P

S

Q

N

T

S

S

L

C

M

A

T

W

E

Q

I

T

K

S

A

-

F

-

R

R

N

N

I

A

A

V

V

V

H

N

S
|
S

L

F

T
x
S

K
|
K

F

Y

Y

Y

A

A

I

M

A

T

G

G

L

W

R
|
R

L

L

G

G

A

W

V

L

I

L

A

V

H

-

P

P

S

T

M

V

I

L

R

R

Q

R

binding glutamic acid: H91 (≠ F67), H212 (≠ I183)
binding pyridoxal-5'-phosphate: G105 (= G79), S106 (≠ A80), S107 (≠ I81), Y131 (≠ F103), N180 (= N153), Y211 (≠ F182), S237 (= S209), S239 (≠ T211), K240 (= K212), R248 (= R220)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 70, 271

P96847 Valine--pyruvate aminotransferase; Alanine--valine transaminase; EC 2.6.1.66 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
31% identity, 46% coverage: 63:233/369 of query aligns to 81:254/388 of P96847

query
sites
P96847

Y

Y

A

Q

A

R

R

R

F

H

G

G

L

I

-

T

-

V

D

E

P

P

V

D

C

A

V

V

L

V

S

I

T

T

N

T

G

G

A

S

I

S

E

G

G

G

L

F

Y

L

L

L

I

A

P

F

R

L

A

A

L

C

-

F

-

D

G

A

I

G

K

D

R

R

V

V

L

A

V

M

P

A

Q

S

P

P

S

G

F

Y

F

P

D

C

Y

Y

G

R

R

N

A

I

C

L

R

S

L

A

A

L

G

G

A

C

E

E

V

V

P

E

L

I

A

P

L

C

S

G

E

P

S

Q

G

T

S

R

F

F

A

Q

F

-

P

P

G

T

I

A

D

Q

K

M

L

L

A

A

D

E

A

I

L

D

A

P

G

P

C

L

D

R

A

G

L

V

A

V

G

A

S

S

P

P

N

A

N

N

P

P

T

T

G

G

T

T

L

V

I

I

P

P

K

P

E

E

L

E

L

L

L

A

A

A

L

I

A

A

-

S

-

W

C

C

R

D

F

A

P

S

E

D

K

V

R

R

F

L

I

I

V

S

D

D

E

E

A

V

F

Y

I

H

Q

G

F

L

T

V

-

Y

E

Q

A

G

F

A

P

P

S

Q

N

T

S

S

L

C

M

A

T

W

E

Q

I

T

K

S

A

-

F

-

R

R

N

N

I

A

A

V

V

V

H

N

S

S

L

F

T

S

K
|
K

F

Y

Y

Y

A

A

I

M

A

T

G

G

L

W

R

R

L

L

G

G

A

W

V

L

I

L

A

V

H

-

P

P

S

T

M

V

I

L

R

R

Q

R

K234 (= K212) modified: N6-(pyridoxal phosphate)lysine

4r5zA Crystal structure of rv3772 encoded aminotransferase (see paper)
28% identity, 40% coverage: 102:248/369 of query aligns to 109:253/353 of 4r5zA

query
sites
4r5zA

S

S

F
|
F

F

E

D

L

Y

Y

G

P

R

P

A

Q

C

V

R

R

L

V

A

A

G

G

A

A

E

I

V

P

V

I

P

Q

L

V

A

P

L

L

S

T

E

D

S

H

G

-

S

-

F

-

A

T

F

F

P

D

G

L

I

Y

D

A

K

M

L

L

A

A

D

T

A

V

L

T

A

D

G

R

C

T

D

R

A

L

L

I

L

F

A

V

G

C

S

N

P

P

N

N

N
|
N

P

P

T

T

G

S

T

T

L

V

I

V

P

G

K

P

E

D

L

A

L

L

L

A

A

R

L

F

A

V

C

E

R

A

F

V

P

P

E

A

K

H

R

I

F

L

I

I

-

A

V

I

D
|
D

E

E

A
|
A

F
x
Y

I

V

Q

E

F

Y

T

I

E

R

A

D

F

G

P

M

S

R

N

P

S

D

L

S

M

L

T

G

E

L

I

V

K

R

A

A

F

H

R

N

N

N

I

V

A

V

V

V

V

L

H

R

S
x
T

L

F

T
x
S

K
|
K

F

A

Y

Y

A

G

I

L

A

A

G

G

L

L

R
|
R

L

I

G

G

A

Y

V

A

I

I

A

G

H

H

P

P

S

D

M

V

I

I

R

T

Q

A

L

L

Y

D

G

K

Y

V

K

Y

E

V

P

P

W

F

T

T

V

V

N

S

A

S

V

I

A

G

E

Q

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: F110 (= F103), N157 (= N153), D184 (= D179), A186 (= A181), Y187 (≠ F182), T214 (≠ S209), S216 (≠ T211), K217 (= K212), R225 (= R220)

Sites not aligning to the query:

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: 84, 85, 86

4r2nA Crystal structure of rv3772 in complex with its substrate (see paper)
28% identity, 40% coverage: 102:248/369 of query aligns to 109:253/353 of 4r2nA

query
sites
4r2nA

S

S

F
|
F

F

E

D
x
L

Y

Y

G

P

R

P

A

Q

C

V

R

R

L

V

A

A

G

G

A

A

E

I

V

P

V

I

P

Q

L

V

A

P

L

L

S

T

E

D

S

H

G

-

S

-

F

-

A

T

F

F

P

D

G

L

I

Y

D

A

K

M

L

L

A

A

D

T

A

V

L

T

A

D

G

R

C

T

D

R

A

L

L

I

L

F

A

V

G

C

S

N

P

P

N

N

N
|
N

P

P

T

T

G

S

T

T

L

V

I

V

P

G

K

P

E

D

L

A

L

L

L

A

A

R

L

F