SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010933579.1 NCBI__GCF_000006985.1:WP_010933579.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 100% coverage: 1:454/456 of query aligns to 3:458/464 of P9WP51

query
sites
P9WP51

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K428 (≠ E423) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
34% identity, 100% coverage: 1:454/456 of query aligns to 1:456/458 of 7xoyA

query
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7xoyA

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binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (≠ Y42), S70 (≠ F70), N72 (≠ T72), T73 (≠ S73), T180 (≠ S180), G182 (≠ A182), T183 (≠ M183), S267 (≠ A273), P293 (≠ T299)

7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
34% identity, 100% coverage: 1:454/456 of query aligns to 1:456/458 of 7xnzB

query
sites
7xnzB

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V

A

F

I

E

G

T

I

M

L

K

R

Q

E

N

Y

D

G

V

V

S

S

Q

Q

M

M

P

P

I

V

V

V

S

G

F

A

G

E

A

P

P

P

I

V

-

M

-

A

-

G

-

E

-

V

-

A

G

G

S

S

I

V

S

S

E

E

N

R

R

E

I

L

L

L

S

S

I

A

L

V

I

F

E

E

N

G

D

R

S

A

A

K

M

L

N

A

A

D

K

A

V

V

V

S

A

A

F

H

M

M

E

S

K

P

P

P

F

L

P

R

V

M

C

I

Q

G

P

A

D

G

A

E

T

L

I

V

S

S

E

A

L

A

S

G

E

K

K

A

L

L

Q

R

T

D

N

W

A

D

S

A

G

-

V

-

L

L

I

M

S

V

L

V

S

E

D

E

G

G

R

K

-

P

L

V

Q

G

L

V

I

I

N

T

K

R

S

Y

D

D

L

L

I

L

D

G

V

F

L

L

T

S

binding pyridoxal-5'-phosphate: K42 (≠ Y42), G179 (≠ A179), T180 (≠ S180), G181 (= G181), G182 (≠ A182), T183 (≠ M183), S267 (≠ A273), P293 (≠ T299), D294 (= D300)

8s5hA Full-length human cystathionine beta-synthase with c-terminal 6xhis- tag, basal state, helical reconstruction (see paper)
28% identity, 95% coverage: 4:434/456 of query aligns to 38:471/507 of 8s5hA

query
sites
8s5hA

D

D

I

I

F

L

D

K

I

K

S

I

D

G

N

D

T

T

P

P

L

M

V

V

L

R

M

I

K

N

Q

K

L

I

T

G

R

K

Q

K

-

F

-

G

K

L

R

K

A

C

R

E

F

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

F

S

N

P

A

C

G

F

G

S

S

H

V

Y

K

C

D

R

R

V

I

S

S

G

L

A

R

I

M

V

I

R

E

D

D

A

A

E

E

E

R

R

D

G

G

A

T

I

L

H

K

P

P

G

G

M

D

T

T

L

I

V

I

D

E

W

P

T

T

F

S

G

G

T

N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

V

A

S

V

R

R

G

G

Y

Y

K

R

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

S

E

E

K

K

Q

V

E

D

V

V

L

L

R

R

A

A

L

L

G

G

A

A

E

E

L

I

V

V

L

R

T

T

P

P

S

T

E

N

A

A

L

R

P

F

G

D

D

S

L

P

Q

E

S

S

C

H

V

V

D

G

V

V

A

A

E

W

N

R

L

L

V

K

R

N

T

E

L

I

P

P

N

N

A

S

W

H

F

I

A

L

N

D

M

Q

Y

Y

Q

R

N

N

P
x
A

V

S

S

N

R

P

Q

L

V

A

H

H

Q
x
Y

D

D

G

T

T

T

G

A

P

D

E

E

I

I

F

L

R

Q

Q

Q

T

C

E

D

G

G

K

K

V

L

T

D

H

M

L

L

F

V

V

A

P

S

M

V

A

G

S

T

G

G

A

G

M

T

I

I

S

T

G

G

I

I

G

A

R
|
R

Y

K

F

L

K

K

S

E

V

K

N

C

P

P

E

G

V

C

Q

R

I

I

G

G

V

V

E

D

P

P

E

E

G

G

S

S

V

I

Y

L

G

A

D

E

L

P

H

E

G

-

G

E

K

L

S

N

Q

Q

G

T

T

E

P

Q

S

T

A

T

F

Y

Q

E

L

V

E

E

D

G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

T

V

V

I

V

D

D

R

K

I

W

V

F

Q

K

V

S

S

N

D

D

A

E

D

E

A

A

F

F

N

T

C

F

G

A

R

R

E

M

L

L

L

I

R

A

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A

S

S

A

G

G

A

S

A

T

M

V

A

A

A

V

A

A

L

V

R

K

E

A

G

A

E

Q

A

E

Y

L

G

Q

D

E

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T

P

D

D

F

S

G

V

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

L

N

S

D

D

D

R

W

W

M

M

R

L

R

Q

Q

K

G

G

F

F

Y

L

R

K

K

E

S

-

K

-

T

-

A

-

L

-

D

-

Q

-

I

-

T

-

A

-

D

E

D

D

I

L

L

T

Q

E

R

K

K

K

A

P

H

W

K

W

D

W

L

H

I

L

F

R

A

V

Q

Q

-

E

-

L

-

G

-

L

-

S

-

A

-

P

-

L

-

T

-

V

-

L

P

P

E

T

Q

I

T

T

L

C

A

G

E

H

V

T

F

I

E

E

T

I

M

L

K

R

Q

E

N

K

D

G

V

F

S

D

Q

Q

M

A

P

P

I

V

V

V

-

D

S

E

F

A

G

G

A

V

P

I

I

L

G

G

S

M

I

V

S

T

E

L

N

G

R

N

I

M

L

L

S

S

I

S

L

L

I

L

E

A

N

G

D

K

S

V

A

Q

M

P

N

S

A

D

K

Q

V

V

V

G

A

K

F

V

M

I

E

Y

K

K

P

Q

F

F

P

K

V

Q

C

I

Q

R

P

L

D

T

A

D

T

T

I

L

S

G

E

R

L

L

S

S

E

H

K

I

L

L

Q

E

T

M

N

D

A

H

S

F

G

A

V

L

L

V

I

V

binding protoporphyrin ix containing fe: A185 (≠ P149), Y192 (≠ Q156), R225 (= R189)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 17, 22, 23, 24

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
28% identity, 95% coverage: 4:434/456 of query aligns to 39:472/500 of 7qgtB

query
sites
7qgtB

D

D

I

I

F

L

D

K

I

K

S

I

D

G

N

D

T

T

P

P

L

M

V

V

L

R

M

I

K

N

Q

K

L

I

T

G

R

K

Q

K

-

F

-

G

K

L

R

K

A

C

R

E

F

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

F

S

N

P

A

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

S

G

L

A

R

I

M

V

I

R

E

D

D

A

A

E

E

E

R

R

D

G

G

A

T

I

L

H

K

P

P

G

G

M

D

T

T

L

I

V

I

D

E

W

P

T
|
T

F
x
S

G

G

T
x
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

V

A

S

V

R

R

G

G

Y

Y

K

R

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

S

E

E

K

K

Q

V

E

D

V

V

L

L

R

R

A

A

L

L

G

G

A

A

E

E

L

I

V

V

L

R

T

T

P

P

S

T

E

N

A

A

L

R

P

F

G

D

D

S

L

P

Q

E

S

S

C

H

V

V

D

G

V

V

A

A

E

W

N

R

L

L

V

K

R

N

T

E

L

I

P

P

N

N

A

S

W

H

F

I

A

L

N

D

M
x
Q

Y

Y

Q

R

N

N

P
x
A

V

S

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

T

T

T

G

A

P

D

E

E

I

I

F

L

R

Q

Q

Q

T

C

E

D

G

G

K

K

V

L

T

D

H

M

L

L

F

V

V

A

P

S

M

V

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

A

R
|
R

Y

K

F

L

K

K

S

E

V

K

N

C

P

P

E

G

V

C

Q

R

I

I

G

G

V

V

E

D

P

P

E

E

G

G

S

S

V

I

Y

L

G

A

D

E

L

P

H

E

G

-

G

E

K

L

S

N

Q

Q

G

T

T

E

P

Q

S

T

A

T

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

T

V

V

I

V

D

D

R

K

I

W

V

F

Q

K

V

S

S

N

D

D

A

E

D

E

A

A

F

F

N

T

C

F

G

A

R

R

E

M

L

L

L

I

R

A

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

A

G

G

A

S

A

T

M

V

A

A

A

V

A

A

L

V

R

K

E

A

G

A

E

Q

A

E

Y

L

G

Q

D

E

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

S

G

V

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

L

N

S

D

D

D

R

W

W

M

M

R

L

R

Q

Q

K

G

G

F

F

Y

L

R

K

K

E

S

-

K

-

T

-

A

-

L

-

D

-

Q

-

I

-

T

-

A

-

D

E

D

D

I

L

L

T

Q

E

R

K

K

K

A

P

H

W

K

W

D

W

L

H

I

L

F

R

A

V

Q

Q

-

E

-

L

-

G

-

L

-

S

-

A

-

P

-

L

-

T

-

V

-

L

P

P

E

T

Q

I

T

T

L

C

A

G

E

H

V

T

F

I

E

E

T

I

M

L

K

R

Q

E

N

K

D

G

V

F

S

D

Q

Q

M

A

P

P

I

V

V

V

-

D

S

E

F

A

G

G

A

V

P

I

I

L

G

G

S

M

I

V

S

T

E

L

N

G

R

N

I

M

L

L

S

S

I

S

L

L

I

L

E

A

N

G

D

K

S

V

A

Q

M

P

N

S

A

D

K

Q

V

V

V

G

A

K

F

V

M

I

E

Y

K

K

P

Q

F

F

P

K

V

Q

C

I

Q

R

P

L

D

T

A

D

T

T

I

L

S

G

E

R

L

L

S

S

E

H

K

I

L

L

Q

E

T

M

N

D

A

H

S

F

G

A

V

L

L

V

I

V

binding protoporphyrin ix containing fe: A186 (≠ P149), P189 (≠ R152), L190 (≠ Q153), Y193 (≠ Q156), R226 (= R189)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (≠ Y42), T106 (= T69), S107 (≠ F70), N109 (≠ T72), T110 (≠ S73), Q182 (≠ M145), G216 (≠ A179), T217 (≠ S180), G218 (= G181), T220 (≠ M183), G265 (≠ D229), S309 (≠ A273), P335 (≠ T299), D336 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

P35520 Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Homo sapiens (Human) (see 40 papers)
28% identity, 95% coverage: 4:434/456 of query aligns to 79:512/551 of P35520

query
sites
P35520

D

D

I

I

F

L

D

K

I

K

S

I

D
x
G

N

D

T
|
T

P

P

L

M

V

V

L

R

M

I

K

N

Q

K

L

I

T

G

R

K

Q

K

-

F

-

G

K
x
L

R
x
K

A

C

R

E

F

L

M

L

A

A

K

K

L
x
C

E

E

Y

F

M

F

S

N

P
x
A

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

S

G

L

A
x
R

I
x
M

V

I

R

E

D

D

A

A

E
|
E

E

R

R

D

G

G

A

T

I

L

H

K

P

P

G
|
G

M

D

T

T

L

I

V
x
I

D
x
E

W

P

T

T

F

S

G
|
G

T
x
N

S

T

G

G

I

I

A

G

L
|
L

A
|
A

M

L

A

A

V

A

S

V

R

R

G

G

Y

Y

K

R

V
x
C

L

I

A

V

A

M

P

P

E

E

S

K

I
x
M

C

S

R

S

E
|
E

K

K

Q

V

E

D

V
|
V

L

L

R

R

A

A

L

L

G

G

A

A

E

E

L

I

V

V

L

R

T
|
T

P

P

S

T

E

N

A

A

L

R

P

F

G

D

D

S

L

P

Q

E

S

S

C

H

V

V

D

G

V

V

A

A

E

W

N

R

L

L

V
x
K

R

N

T

E

L

I

P

P

N

N

A

S

W

H

F

I

A

L

N

D

M

Q

Y

Y

Q

R

N

N

P
x
A

V

S

S
x
N

R

P

Q

L

V
x
A

H

H

Q

Y

D
|
D

G

T

T

T

G

A

P

D

E

E

I

I

F

L

R

Q

Q

Q

T

C

E

D

G

G

K

K

V

L

T

D

H

M

L

L

F

V

V

A

P

S

M

V

A
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S
x
T

G

G

I

I

G

A

R
|
R

Y

K

F

L

K
|
K

S

E

V

K

N
x
C

P

P

E

G

V
x
C

Q

R

I

I

I
|
I

G

G

V

V

E
x
D

P

P

E

E

G

G

S

S

V

I

Y

L

G
x
A

D

E

L

P

H

E

G

-

G

E

K

L

S

N

Q

Q

G

T

T

E

P

Q

S

T

A

T

F

Y

Q
x
E

L

V

E

E

D
x
G

I

I

G
|
G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

T

V

V

I
x
V

D
|
D

R

K

I

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G85 (≠ D10) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T12) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (≠ K24) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (≠ R25) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ L32) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P37) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (≠ Y42) modified: N6-(pyridoxal phosphate)lysine
R125 (≠ A48) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (≠ I49) to V: in CBSD; loss of activity
E131 (= E54) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G62) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (≠ V66) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (≠ D67) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G71) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (≠ T72) binding pyridoxal 5'-phosphate
L154 (= L77) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A78) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (≠ V88) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ I96) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (= E99) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (= V103) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (= T114) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ V134) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ P149) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (≠ S151) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (≠ V154) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (= D157) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (≠ ASGAM 179:183) binding pyridoxal 5'-phosphate
T257 (≠ S180) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ S185) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (= R189) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (= K192) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ N195) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ V198) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (= I201) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (≠ E204) to N: in CBSD; loss of activity
A288 (≠ G211) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (≠ Q226) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (≠ D229) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G231) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ I244) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D245) to V: in CBSD; loss of activity
R336 (= R260) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (= L262) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G271) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (≠ A273) binding pyridoxal 5'-phosphate; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ A277) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ L293) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D300) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (≠ G303) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (≠ R308) to E: in CBSD; severe form; dbSNP:rs121964967
P422 (vs. gap) to L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
P427 (= P350) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
I435 (≠ F358) to T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
R439 (≠ K362) to Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
D444 (≠ S367) to N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
V449 (= V372) to G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
L456 (≠ I378) to P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
S466 (= S388) to L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
S500 (= S422) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
78 P → R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

6xylA Crystal structure of delta466-491 cystathionine beta-synthase from toxoplasma gondii with l-serine (see paper)
29% identity, 100% coverage: 1:456/456 of query aligns to 8:467/468 of 6xylA

query
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binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K51 (≠ Y42), T82 (≠ S73), Q154 (≠ M145), G188 (≠ A179), T189 (≠ S180), G190 (= G181), T192 (≠ M183), G238 (≠ D229), I239 (= I230), Y241 (≠ A232), S282 (≠ A273), P308 (≠ T299), D309 (= D300)

6xwlC Cystathionine beta-synthase from toxoplasma gondii (see paper)
29% identity, 100% coverage: 1:456/456 of query aligns to 8:476/477 of 6xwlC

query
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A

N

K

L

L

V

Q

R

R

T

E

L

L

P

E

N

N

A

A

W

H

F

I

A

L

N

D

M

Q

Y

Y

Q

N

N

N

P

T

V

A

S

N

R

P

Q

M

V

V

H

H

Q

Y

D

D

G

V

T

T

G

A

P

E

E

E

I

I

F

I

R

T

Q

Q

T

C

E

D

G

G

K

D

V

I

T

D

H

M

L

V

F

V

V

I

P

G

M

A

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

G

R

R

Y

K

F

I

K

K

S

E

V

R

N

C

P

P

E

K

V

C

Q

K

I

V

G

G

V

V

E

D

P

P

E

K

G

G

S

S

V

I

Y

L

G

A

D

V

L

P

H

D

G

S

G

L

K

N

S

D

Q

E

G

K

T

R

P

L

S

Q

A

S

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

V

T

P

S

G

F

V

W

L

D

D

P

R

N

K

V

V

I

V

D

D

R

E

I

W

V

V

Q

K

V

V

S

G

D

D

A

A

D

E

A

S

F

F

N

T

C

T

G

A

R

R

E

A

L

I

R

R

A

N

E

E

A

G

V

L

F

F

A

V

G

G

A
x
S

S

S

G

G

A

A

A

N

M

V

A

W

A

G

A

A

L

L

R

Q

E

A

G

A

E

R

A

Q

Y

L

G

K

D

K

D

G

A

Q

L

K

V

C

V

V

M

L

T
x
P

D
|
D

F

S

G

S

G

R

Y

N

D

Y

L

M

S

S

R

K

M

F

Y

I

N

S

D

D

D

E

W

W

M

M

R

A

R

E

Q

H

G

G

F

F

-

A

-

P

-

E

-

D

-

G

-

A

-

K

Y

V

R

K

K

E

S

R

K

E

T

K

A

Q

L

F

D

G

Q

G

I

A

T

R

A

I

D

R

D

D

I

L

L

L

-

S

Q

E

R

T

K

G

A

A

H

T

K

S

D

D

L

V

I

P

F

F

A

V

Q

T

P

A

E

R

Q

L

T

S

L

V

A

E

E

D

V

V

F

I

E

K

T

M

M

M

K

H

Q

E

N

T

D

K

V

V

S

K

Q

E

M

V

P

I

I

V

V

T

-

E

-

D

S

S

F

E

G

G

A

K

P

T

-

K

-

L

I

V

G

G

S

V

I

L

S

S

E

E

N

D

R

H

I

I

L

A

S

H

I

S

L

L

I

Q

E

S

N

G

D

R

S

C

A

A

M

M

N

Q

A

S

K

P

V

V

V

K

A

D

F

I

M

A

E

F

K

K

P

K

F

L

P

A

V

K

C

A

Q

L

P

P

D

S

A

A

T

Y

I

L

S

R

E

D

L

V

S

A

E

K

K

A

L

L

Q

D

T

F

N

S

A

P

S

Y

G

-

V

V

L

C

I

V

S

M

L

D

S

C

D

P

G

H

R

F

L

L

Q

G

L

V

I

I

N

T

K

R

S

I

D

D

L

L

I

L

D

H

V

W

L

L

T

A

R

T

K

K

binding pyridoxal-5'-phosphate: K51 (≠ Y42), N81 (≠ T72), G188 (≠ A179), T189 (≠ S180), G190 (= G181), T192 (≠ M183), G238 (≠ D229), S282 (≠ A273), P308 (≠ T299), D309 (= D300)

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
28% identity, 100% coverage: 1:454/456 of query aligns to 39:496/504 of 3pc4A

query
sites
3pc4A

M

I

T

T

N

P

D

N

I

I

F

L

D

E

I

V

S

I

D

G

N

C

T

T

P

P

L

L

V

V

L

K

M

L

K

N

Q

N

L

I

T

P

R

A

Q

S

K

D

-

G

-

I

R

E

A

C

R

E

F

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

S

N

P

P

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

G

G

Y

A

R

I

M

V

V

R

Q

D

D

A

A

E

E

R

Q

G

G

A

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T
|
T

F
x
S

G

G

T
x
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

A

C

V

A

S

V

R

K

G

G

Y

Y

K

K

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

A

L

L

R

R

A

T

L

L

G

G

A

A

E

K

L

I

V

I

L

R

T

T

P

P

S

T

E

E

A

A

L

A

P

Y

G

D

D

S

L

P

Q

E

S

G

C

L

V

I

D

Y

V

V

A

A

E

Q

N

Q

L

L

V

Q

R

R

T

E

L

T

P

P

N

N

A

S

W

I

F

V

A

L

N

D

M
x
Q

Y

Y

Q

R

N

N

P
x
A

V

G

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

G

T

T

G

A

P

A

E

E

I

I

F

L

R

W

Q

Q

T

L

E

D

G

N

K

K

V

V

T

D

H

M

L

I

F

V

V

V

P

S

M
x
A

A
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

R
|
R

Y

K

F

I

K

K

S

E

V

Q

N

V

P

P

E

S

V

C

Q

Q

I

I

I

V

G

G

V

V

E

D

P

P

E

Y

G

G

S

S

V

I

Y

L

-

A

-

R

-

P

G

A

D

E

L

L

H

N

G

K

G

T

K

D

S

V

Q

Q

G

-

T

-

P

-

S

-

A

F

F

Y

Q

E

L

V

E

E

D
x
G

I
|
I

G

G

A
x
Y

V

D

R

F

P

P

T

P

S
x
T

F

V

W

F

D

D

P

D

N

T

V

V

I

V

D

D

R

V

I

W

V

T

Q

K

V

I

S

G

D

D

A

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

A

A

M

M

A

H

A

A

L

L

R

E

E

H

G

A

E

R

A

K

Y

L

G

K

D

K

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

G

G

I

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

V

N

S

D

D

D

N

W

W

M

M

R

E

R

A

Q

R

G

N

F

F

Y

K

R

E

K

P

S

V

K

N

T

E

A

H

L

G

D

H

Q

W

I

W

T

W

A

S

D

L

D

A

I

I

L

A

Q

E

R

L

K

E

A

L

H

P

K

A

D

P

L

P

I

V

F

I

A

L

Q

K

P

S

E

D

Q

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

T

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

M

L

P

P

I

V

V

V

S

D

F

Q

-

D

G

G

A

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

R

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

S

R

A

Q

M

Q

N

S

A

D

K

P

V

A

V

I

A

K

F

A

M

L

E

N

K

K

P

R

F

V

P

I

V

R

C

L

Q

N

P

E

D

S

A

E

T

I

I

L

S

G

E

K

L

L

S

A

E

R

K

V

L

L

Q

E

T

V

N

D

A

P

S

S

G

V

V

L

L

I

I

L

S

G

L

K

S

N

D

P

-

A

G

G

R

K

L

V

Q

E

L

L

I

K

N

A

K

L

S

A

D

T

L

K

I

L

D

D

V

V

L

T

T

T

active site: K82 (≠ Y42), S312 (≠ A273)
binding protoporphyrin ix containing fe: A189 (≠ P149), P192 (≠ R152), L193 (≠ Q153), Y196 (≠ Q156), R229 (= R189), T276 (≠ S237)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (≠ Y42), T109 (= T69), S110 (≠ F70), N112 (≠ T72), T113 (≠ S73), Q185 (≠ M145), A218 (≠ M178), G219 (≠ A179), T220 (≠ S180), A221 (≠ G181), T223 (≠ M183), G268 (≠ D229), I269 (= I230), Y271 (≠ A232), S312 (≠ A273), P338 (≠ T299), D339 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
28% identity, 100% coverage: 1:454/456 of query aligns to 39:496/504 of 3pc3A

query
sites
3pc3A

M

I

T

T

N

P

D

N

I

I

F

L

D

E

I

V

S

I

D

G

N

C

T

T

P

P

L

L

V

V

L

K

M

L

K

N

Q

N

L

I

T

P

R

A

Q

S

K

D

-

G

-

I

R

E

A

C

R

E

F

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

S

N

P

P

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

G

G

Y

A

R

I

M

V

V

R

Q

D

D

A

A

E

E

R

Q

G

G

A

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T
|
T

F
x
S

G

G

T
x
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

A

C

V

A

S

V

R

K

G

G

Y

Y

K

K

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

A

L

L

R

R

A

T

L

L

G

G

A

A

E

K

L

I

V

I

L

R

T

T

P

P

S

T

E

E

A

A

L

A

P

Y

G

D

D

S

L

P

Q

E

S

G

C

L

V

I

D

Y

V

V

A

A

E

Q

N

Q

L

L

V

Q

R

R

T

E

L

T

P

P

N

N

A

S

W

I

F

V

A

L

N

D

M
x
Q

Y

Y

Q

R

N

N

P
x
A

V

G

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

G

T

T

G

A

P

A

E

E

I

I

F

L

R

W

Q

Q

T

L

E

D

G

N

K

K

V

V

T

D

H

M

L

I

F

V

V

V

P

S

M
x
A

A
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

R
|
R

Y

K

F

I

K

K

S

E

V

Q

N

V

P

P

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S

V

C

Q

Q

I

I

I

V

G

G

V

V

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D

P

P

E

Y

G

G

S

S

V

I

Y

L

-

A

-

R

-

P

G

A

D

E

L

L

H

N

G

K

G

T

K

D

S

V

Q

Q

G

-

T

-

P

-

S

-

A

F

F

Y

Q

E

L

V

E

E

D
x
G

I
|
I

G

G

A

Y

V

D

R

F

P

P

T

P

S

T

F

V

W

F

D

D

P

D

N

T

V

V

I

V

D

D

R

V

I

W

V

T

Q

K

V

I

S

G

D

D

A

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

A

A

M

M

A

H

A

A

L

L

R

E

E

H

G

A

E

R

A

K

Y

L

G

K

D

K

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

G

G

I

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

V

N

S

D

D

D

N

W

W

M

M

R

E

R

A

Q

R

G

N

F

F

Y

K

R

E

K

P

S

V

K

N

T

E

A

H

L

G

D

H

Q

W

I

W

T

W

A

S

D

L

D

A

I

I

L

A

Q

E

R

L

K

E

A

L

H

P

K

A

D

P

L

P

I

V

F

I

A

L

Q

K

P

S

E

D

Q

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

T

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

M

L

P

P

I

V

V

V

S

D

F

Q

-

D

G

G

A

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

R

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

S

R

A

Q

M

Q

N

S

A

D

K

P

V

A

V

I

A

K

F

A

M

L

E

N

K

K

P

R

F

V

P

I

V

R

C

L

Q

N

P

E

D

S

A

E

T

I

I

L

S

G

E

K

L

L

S

A

E

R

K

V

L

L

Q

E

T

V

N

D

A

P

S

S

G

V

V

L

L

I

I

L

S

G

L

K

S

N

D

P

-

A

G

G

R

K

L

V

Q

E

L

L

I

K

N

A

K

L

S

A

D

T

L

K

I

L

D

D

V

V

L

T

T

T

active site: K82 (≠ Y42), S312 (≠ A273)
binding protoporphyrin ix containing fe: A189 (≠ P149), P192 (≠ R152), L193 (≠ Q153), Y196 (≠ Q156), R229 (= R189)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (≠ Y42), T109 (= T69), S110 (≠ F70), N112 (≠ T72), T113 (≠ S73), Q185 (≠ M145), A218 (≠ M178), G219 (≠ A179), T220 (≠ S180), A221 (≠ G181), T223 (≠ M183), G268 (≠ D229), I269 (= I230), S312 (≠ A273), P338 (≠ T299), D339 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
28% identity, 100% coverage: 1:454/456 of query aligns to 37:494/500 of 3pc2A

query
sites
3pc2A

M

I

T

T

N

P

D

N

I

I

F

L

D

E

I

V

S

I

D

G

N

C

T

T

P

P

L

L

V

V

L

K

M

L

K

N

Q

N

L

I

T

P

R

A

Q

S

K

D

-

G

-

I

R

E

A

C

R

E

F

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

S

N

P

P

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

G

G

Y

A

R

I

M

V

V

R

Q

D

D

A

A

E

E

R

Q

G

G

A

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T

T

F

S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

A

C

V

A

S

V

R

K

G

G

Y

Y

K

K

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

A

L

L

R

R

A

T

L

L

G

G

A

A

E

K

L

I

V

I

L

R

T

T

P

P

S

T

E

E

A

A

L

A

P

Y

G

D

D

S

L

P

Q

E

S

G

C

L

V

I

D

Y

V

V

A

A

E

Q

N

Q

L

L

V

Q

R

R

T

E

L

T

P

P

N

N

A

S

W

I

F

V

A

L

N

D

M

Q

Y

Y

Q

R

N

N

P
x
A

V

G

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

G

T

T

G

A

P

A

E

E

I

I

F

L

R

W

Q

Q

T

L

E

D

G

N

K

K

V

V

T

D

H

M

L

I

F

V

V

V

P

S

M
x
A

A
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

R
|
R

Y

K

F

I

K

K

S

E

V

Q

N

V

P

P

E

S

V

C

Q

Q

I

I

I

V

G

G

V

V

E

D

P

P

E

Y

G

G

S

S

V

I

Y

L

-

A

-

R

-

P

G

A

D

E

L

L

H

N

G

K

G

T

K

D

S

V

Q

Q

G

-

T

-

P

-

S

-

A

F

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

P

T

P

S

T

F

V

W

F

D

D

P

D

N

T

V

V

I

V

D

D

R

V

I

W

V

T

Q

K

V

I

S

G

D

D

A

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

A

A

M

M

A

H

A

A

L

L

R

E

E

H

G

A

E

R

A

K

Y

L

G

K

D

K

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

G

G

I

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

V

N

S

D

D

D

N

W

W

M

M

R

E

R

A

Q

R

G

N

F

F

Y

K

R

E

K

P

S

V

K

N

T

E

A

H

L

G

D

H

Q

W

I

W

T

W

A

S

D

L

D

A

I

I

L

A

Q

E

R

L

K

E

A

L

H

P

K

A

D

P

L

P

I

V

F

I

A

L

Q

K

P

S

E

D

Q

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

T

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

M

L

P

P

I

V

V

V

S

D

F

Q

-

D

G

G

A

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

R

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

S

R

A

Q

M

Q

N

S

A

D

K

P

V

A

V

I

A

K

F

A

M

L

E

N

K

K

P

R

F

V

P

I

V

R

C

L

Q

N

P

E

D

S

A

E

T

I

I

L

S

G

E

K

L

L

S

A

E

R

K

V

L

L

Q

E

T

V

N

D

A

P

S

S

G

V

V

L

L

I

I

L

S

G

L

K

S

N

D

P

-

A

G

G

R

K

L

V

Q

E

L

L

I

K

N

A

K

L

S

A

D

T

L

K

I

L

D

D

V

V

L

T

T

T

active site: K80 (≠ Y42), S310 (≠ A273)
binding protoporphyrin ix containing fe: A187 (≠ P149), P190 (≠ R152), L191 (≠ Q153), Y194 (≠ Q156), R227 (= R189)
binding pyridoxal-5'-phosphate: K80 (≠ Y42), N110 (≠ T72), A216 (≠ M178), G217 (≠ A179), T218 (≠ S180), A219 (≠ G181), T221 (≠ M183), G266 (≠ D229), S310 (≠ A273), P336 (≠ T299), D337 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26

4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
28% identity, 95% coverage: 4:434/456 of query aligns to 37:466/486 of 4pcuA

query
sites
4pcuA

D

D

I

I

F

L

D

K

I

K

S

I

D

G

N

D

T

T

P

P

L

M

V

V

L

R

M

I

K

N

Q

K

L

I

T

G

R

K

Q

K

-

F

-

G

K

L

R

K

A

C

R

E

F

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

F

S

N

P

A

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

S

G

L

A

R

I

M

V

I

R

E

D

D

A

A

E

E

E

R

R

D

G

G

A

T

I

L

H

K

P

P

G

G

M

D

T

T

L

I

V

I

D

E

W

P

T

T

F
x
S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

V

A

S

V

R

R

G

G

Y

Y

K

R

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

S

E

E

K

K

Q

V

E

D

V

V

L

L

R

R

A

A

L

L

G

G

A

A

E

E

L

I

V

V

L

R

T

T

P

P

S

A

E

R

A

F

L

-

P

-

G

D

D

S

L

P

Q

S

S

S

C

H

V

V

D

G

V

V

A

A

E

W

N

R

L

L

V

K

R

N

T

E

L

I

P

P

N

N

A

S

W

H

F

I

A

L

N

D

M

Q

Y

Y

Q

R

N

N

P
x
A

V

S

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

T

T

T

G

A

P

D

E

E

I

I

F

L

R

Q

Q

Q

T

C

E

D

G

G

K

K

V

L

T

D

H

M

L

L

F

V

V

A

P

S

M

V

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

A

R
|
R

Y

K

F

L

K

K

S

E

V

K

N

C

P

P

E

G

V

C

Q

R

I

I

G

G

V

V

E
x
D

P

P

E

E

G

G

S

S

V

I

Y

L

G

A

D

E

L

P

H

E

G

-

G

E

K

L

S

N

Q

Q

G

T

T

E

P

Q

S

T

A

T

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S
x
T

F

V

W

L

D

D

P

R

N

T

V

V

I

V

D

D

R

K

I

W

V

F

Q

K

V

S

S

N

D

D

A

E

D

E

A

A

F

F

N

T

C

F

G

A

R

R

E

M

L

L

L

I

R

A

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

A

G

G

A

S

A

T

M

V

A

A

A

V

A

A

L

V

R

K

E

A

G

A

E

Q

A

E

Y

L

G

Q

D

E

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

S

G

V

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

L

N

S

D

D

D

R

W

W

M

M

R

L

R

Q

Q

K

G

G

F

F

Y

L

R

K

K

E

S

E

K

D

T

L

A

K

L

K

D

P

Q

W

I

W

T

W

A

H

D

L

D

R

I

V

L

-

Q

Q

R

E

K

L

A

G

H

L

K

S

D

A

L
x
P

I

L

F

T

A

V

Q

L

P

P

E

T

Q

I

T

T

L

C

A

G

E

H

V

T

F

I

E

E

T

I

M

L

K

R

Q

E

N

K

D
x
G

V
x
F

S
x
D

Q
|
Q

M

A

P

P

I

V

V

V

-

D

S

E

F

A

G

G

A

V

P

I

I

L

G

G

S

M

I

V

S

T

E

L

N

G

R

N

I

M

L

L

S

S

I

S

L

L

I

L

E

A

N

G

D

K

S

V

A

Q

M

P

N

S

A

D

K

Q

V

V

V

G

A

K

F

V

M

I

E

Y

K

K

P

Q

F

F

P

K

V

Q

C

I

Q

R

P

L

D

T

A

D

T

T

I

L

S

G

E

R

L

L

S

S

E

H

K

I

L

L

Q

E

T

M

N

D

A

H

S

F

G

A

V

L

L

V

I

V

active site: K77 (≠ Y42), S105 (≠ F70), D237 (≠ E204), S305 (≠ A273)
binding protoporphyrin ix containing fe: A182 (≠ P149), P185 (≠ R152), L186 (≠ Q153), Y189 (≠ Q156), R222 (= R189), T269 (≠ S237)
binding pyridoxal-5'-phosphate: K77 (≠ Y42), N107 (≠ T72), G212 (≠ A179), T213 (≠ S180), G214 (= G181), T216 (≠ M183), G261 (≠ D229), S305 (≠ A273), P331 (≠ T299), D332 (= D300)
binding s-adenosylmethionine: P376 (≠ L345), G396 (≠ D365), F397 (≠ V366), D398 (≠ S367), Q399 (= Q368)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 20, 21, 22, 23
binding s-adenosylmethionine: 476, 478, 479

Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
29% identity, 99% coverage: 4:455/456 of query aligns to 38:494/504 of Q2V0C9

query
sites
Q2V0C9

D

D

I

I

F

L

D

T

I

A

S

I

D

G

N

Q

T

T

P

P

L

L

V

I

L

K

M

L

K

N

Q

N

L

I

T

P

R

K

Q

S

K

Y

-

G

-

I

R

K

A

C

R

E

F

I

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

S

N

P

P

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

A

G

Y

A

R

I

M

V

I

R

Q

D

D

A

A

E

E

E

D

R

K

G

G

A

L

I

L

H

K

P

P

G

G

M

C

T

T

L

I

V

I

D

E

W

P

T

T

F

S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

V

A

S

V

R

R

G

G

Y

Y

K

K

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

D

E

E

K

K

Q

I

E

S

V

T

L

L

R

Y

A

A

L

L

G

G

A

A

E

K

L

I

V

I

L

R

T

T

P

P

S

T

E

E

A

A

L

S

P

W

G

H

D

S

L

P

Q

E

S

A

C

H

V

I

D

S

V

V

A

A

E

Q

N

K

L

L

V

Q

R

K

T

E

L

I

P

P

N

N

A

S

W

I

F

I

A

L

N

D

M

Q

Y

Y

Q

T

N

N

P

P

V

G

S

N

R

P

Q

L

V

A

H

H

Q

Y

D

D

G

Q

T

T

G

A

P

I

E

E

I

I

F

W

R

K

Q

Q

T

C

E

E

G

G

K

K

V

I

T

D

H

Y

L

L

F

V

V

A

P

G

M

A

A
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

S

G

G

I

I

G

G

R

R

Y

K

F

L

K

K

S

E

V

L

N

S

P

P

E

N

V

I

Q

K

I

I

G

A

V

V

E

D

P

P

E

K

G

G

S

S

V

I

Y

L

G

D

D

P

L

-

H

-

G

S

K

D

S

S

Q

Q

G

N

T

E

P

V

S

G

A

F

F

Y

Q

E

L

V

E

E

D

G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

N

V

V

I

I

D

D

R

K

I

W

V

I

Q

K

V

T

S

E

D

D

A

N

D

E

A

S

F

L

N

N

C

A

G

A

R

R

E

M

L

L

L

I

R

R

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

A

M

L

A

I

A

A

L

L

R

K

E

I

G

A

E

K

A

D

Y

I

G

P

D

E

A

K

L

R

V

M

V

V

V

I

M

I

M

L

T

P

D

D

F

G

G

I

G

R

Y

N

D

Y

L

L

S

T

R

K

M

F

Y

V

N

S

D

E

D

Y

W

W

M

M

R

E

R

T

Q

R

G

G

F

F

Y

L

R

Q

K

P

-

V

S

C

K

Q

T

N

A

E

L

M

D

N

Q

K

I

W

T

W

A

W

D

N

-

M

D

K

I

I

L

S

Q

N

R

L

K

S

A

F

H

D

K

K

D

Q

L

S

I

L

F

L

A

K

Q

E

P

N

E

T

Q

V

T

T

L

C

A

Q

E

E

V

A

F

M

E

H

T

M

M

L

K

K

Q

N

N

A

D

D

V

S

S

Q

Q

L

M

L

P

V

I

I

V

S

S

D

F

D

G

N

A

I

P

H

I

I

-

K

G

G

S

V

I

I

S

S

E

L

N

N

R

K

I

L

L

T

S

S

I

Y

L

V

I

I

E

S

N

G

D

I

S

V

A

K

M

C

N

T

A

D

K

F

V

V

V

D

A

K

F

A

M

M

E

V

K

K

P

Q

F

Y

P

V

V

K

C

V

Q

K

P

H

D

S

A

A

T

T

I

L

S

G

E

Y

L

I

S

S

E

R

K

V

L

L

Q

E

T

K

N

E

A

P

S

Y

G

V

V

I

L

I

I

L

-

D

S

E

L

H

S

D

D

D

G

A

R

F

L

I

Q

G

L

I

I

V

N

N

K

Q

S

F

D

H

L

I

I

L

D

Q

V

F

L

I

T

T

R

K

K78 (≠ Y42) modified: N6-(pyridoxal phosphate)lysine
N108 (≠ T72) binding pyridoxal 5'-phosphate
GTGGT 215:219 (≠ ASGAM 179:183) binding pyridoxal 5'-phosphate
S307 (≠ A273) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

12 binding axial binding residue
23 binding axial binding residue

1jbqA Structure of human cystathionine beta-synthase: a unique pyridoxal 5'- phosphate dependent hemeprotein (see paper)
33% identity, 70% coverage: 4:320/456 of query aligns to 37:347/348 of 1jbqA

query
sites
1jbqA

D

D

I

I

F

L

D

K

I

K

S

I

D

G

N

D

T

T

P

P

L

M

V

V

L

R

M

I

K

N

Q

K

L

I

T

G

R

K

Q

K

-

F

-

G

K

L

R

K

A

C

R

E

F

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

F

S

N

P

A

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

S

G

L

A

R

I

M

V

I

R

E

D

D

A

A

E

E

E

R

R

D

G

G

A

T

I

L

H

K

P

P

G

G

M

D

T

T

L

I

V

I

D

E

W

P

T

T

F
x
S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

V

A

S

V

R

R

G

G

Y

Y

K

R

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

S

E

E

K

K

Q

V

E

D

V

V

L

L

R

R

A

A

L

L

G

G

A

A

E

E

L

I

V

V

L

R

T

T

P

P

S

T

E

E

A

-

L

-

P

-

G

-

D

-

L

-

Q

-

S

S

C

H

V

V

D

G

V

V

A

A

E

W

N

R

L

L

V

K

R

N

T

E

L

I

P

P

N

N

A

S

W

H

F

I

A

L

N

D

M

Q

Y

Y

Q

R

N

N

P
x
A

V

S

S

N

R
x
P

Q
x
L

V

A

H

H

Q
x
Y

D

D

G

T

T

T

G

A

P

D

E

E

I

I

F

L

R

Q

Q

Q

T

C

E

D

G

G

K

K

V

L

T

D

H

M

L

L

F

V

V

A

P

S

M
x
V

A
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

T

G

G

I

I

G

A

R
|
R

Y

K

F

L

K

K

S

E

V

K

N

C

P

P

E

G

V

C

Q

R

I

I

G

G

V

V

E
x
D

P

P

E

E

G

G

S
|
S

V

I

Y
x
L

G

A

D

E

L

P

H

E

G

-

G

E

K

L

S

N

Q

Q

G

T

T

E

P

Q

S

T

A

T

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

T

V

V

I

V

D

D

R

K

I

W

V

F

Q

K

V

S

S

N

D

D

A

E

D

E

A

A

F

F

N

T

C

F

G

A

R

R

E

M

L

L

L

I

R

A

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

A

G

G

A

S

A

T

M

V

A

A

A

V

A

A

L

V

R

K

E

A

G

A

E

Q

A

E

Y

L

G

Q

D

E

D

G

A

Q

L

R

V

C

V

V

M

I

M

L

T
x
P

D
|
D

F

S

G

V

G

R

Y

N

D

Y

L

M

S

T

R

K

M

F

Y

L

N

S

D

D

D

R

W

W

M

M

R

L

R

Q

Q

K

G

G

F

F

active site: K77 (≠ Y42), S105 (≠ F70), D232 (≠ E204), S236 (= S208), L238 (≠ Y210), S300 (≠ A273), P326 (≠ T299)
binding protoporphyrin ix containing fe: A177 (≠ P149), P180 (≠ R152), L181 (≠ Q153), Y184 (≠ Q156), R217 (= R189)
binding pyridoxal-5'-phosphate: K77 (≠ Y42), N107 (≠ T72), V206 (≠ M178), G207 (≠ A179), T208 (≠ S180), G209 (= G181), G210 (≠ A182), T211 (≠ M183), G256 (≠ D229), S300 (≠ A273), P326 (≠ T299), D327 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 9, 10, 11, 12, 16, 20, 21, 22, 23

5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
29% identity, 99% coverage: 4:455/456 of query aligns to 34:487/488 of 5ohxA

query
sites
5ohxA

D

D

I

I

F

L

D

T

I

A

S

I

D

G

N

Q

T

T

P

P

L

L

V

I

L

K

M

L

K

N

Q

N

L

I

T

P

R

K

Q

S

K

Y

-

G

-

I

R

K

A

C

R

E

F

I

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

S

N

P

P

C

G

F

G

S

S

H

V

Y
x
K

C

D

R

R

V

I

S

A

G

Y

A

R

I

M

V

I

R

Q

D

D

A

A

E

E

E

D

R

K

G

G

A

L

I

L

H

K

P

P

G

G

M

C

T

T

L

I

V

I

D

E

W

P

T

T

F

S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

V

A

S

V

R

R

G

G

Y

Y

K

K

V

C

L

I

A

V

A

M

P

P

E

E

S

K

I

M

C

S

R

D

E

E

K

K

Q

I

E

S

V

T

L

L

R

Y

A

A

L

L

G

G

A

A

E

K

L

I

V

I

L

R

T

T

P

P

S

T

E

E

A

A

L

S

P

W

G

H

D

S

L

P

Q

E

S

A

C

H

V

I

D

S

V

V

A

A

E

Q

N

K

L

L

V

Q

R

K

T

E

L

I

P

P

N

N

A

S

W

I

F

I

A

L

N

D

M

Q

Y

Y

Q

T

N

N

P
|
P

V

G

S

N

R
x
P

Q

L

V

A

H

H

Q
x
Y

D

D

G

Q

T

T

G

A

P

I

E

E

I

I

F

W

R

K

Q

Q

T

C

E

E

G

G

K

K

V

I

T

D

H

Y

L

L

F

V

V

A

P
x
G

M

A

A
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

S

G

G

I

I

G

G

R
|
R

Y

K

F

L

K

K

S

E

V

L

N

S

P

P

E

N

V

I

Q

K

I

I

G

A

V

V

E

D

P

P

E

K

G

G

S

S

V

I

Y

L

G

-

D

-

L

-

H

-

G

-

G

D

K

P

S

S

Q

S

G

D

T

E

P

V

S

G

A

F

F

Y

Q

E

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

I

T

P

S

T

F

V

W

L

D

D

P

R

N

N

V

V

I

I

D

D

R

K

I

W

V

I

Q

K

V

T

S

E

D

D

A

N

D

E

A

S

F

L

N

N

C

A

G

A

R

R

E

M

L

L

L

I

R

R

A

Q

E

E

A

G

V

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

A

M

L

A

I

A

A

L

L

R

K

E

I

G

A

E

K

A

D

Y

I

G

P

D

E

A

K

L

R

V

M

V

V

V

I

M

I

M

L

T
x
P

D
|
D

F

G

G

I

G

R

Y

N

D

Y

L

L

S

T

R

K

M

F

Y

V

N

S

D

E

D

Y

W

W

M

M

R

E

R

T

Q

R

G

G

F

F

Y

L

R

Q

K

P

-

V

S

C

K

Q

T

N

A

E

L

M

D

N

Q

K

I

W

T

W

A

W

D

N

-

M

D

K

I

I

L

S

Q

N

R

L

K

S

A

F

H

D

K

K

D

Q

L

S

I

L

F

L

A

K

Q

E

P

N

E

T

Q

V

T

T

L

C

A

Q

E

E

V

A

F

M

E

H

T

M

M

L

K

K

Q

N

N

A

D

D

V

S

S

Q

Q

L

M

L

P

V

I

I

V

S

S

D

F

D

G

N

A

I

P

H

I

I

-

K

G

G

S

V

I

I

S

S

E

L

N

N

R

K

I

L

L

T

S

S

I

Y

L

V

I

I

E

S

N

G

D

I

S

V

A

K

M

C

N

T

A

D

K

F

V

V

V

D

A

K

F

A

M

M

E

V

K

K

P

Q

F

Y

P

V

V

K

C

V

Q

K

P

H

D

S

A

A

T

T

I

L

S

G

E

Y

L

I

S

S

E

R

K

V

L

L

Q

E

T

K

N

E

A

P

S

Y

G

V

V

I

L

I

I

L

-

D

S

E

L

H

S

D

D

D

G

A

R

F

L

I

Q

G

L

I

I

V

N

N

K

Q

S

F

D

H

L

I

I

L

D

Q

V

F

L

I

T

T

R

K

binding protoporphyrin ix containing fe: P181 (= P149), P184 (≠ R152), Y188 (≠ Q156), R221 (= R189)
binding pyridoxal-5'-phosphate: K74 (≠ Y42), N104 (≠ T72), G209 (≠ P177), G211 (≠ A179), T212 (≠ S180), G213 (= G181), G214 (≠ A182), T215 (≠ M183), G256 (≠ D229), S300 (≠ A273), P326 (≠ T299), D327 (= D300)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 4, 7, 8, 9, 10, 14, 16, 17, 18, 19

6c2zA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-aminoacrylate intermediate (see paper)
31% identity, 70% coverage: 3:321/456 of query aligns to 9:337/345 of 6c2zA

query
sites
6c2zA

N

H

D

N

I

V

F

I

D

D

I

L

S

V

D

G

N

N

T

T

P

P

L

L

V

I

L

A

M

L

K

K

Q

K

L

L

T

P

R

K

Q

A

K

L

-

G

-

I

R

K

A

P

R

Q

F

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

S

N

P

P

C

G

F

G

S

S

H

I

Y
x
K

C

D

R

R

V

I

S

A

G

K

A

S

I

M

V

V

R

E

D

E

A

A

E

E

E

A

R

S

G

G

A

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T
|
T

F
x
S

G

G

T
x
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

A

G

V

A

S

I

R

K

G

G

Y

Y

K

R

V

T

L

I

A

T

A

L

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

V

L

L

R

K

A

A

L

L

G

G

A

A

E

E

L

I

V

I

L

R

T

T

P

P

S

T

E

A

A

A

L

A

P

W

G

D

D

S

L

P

Q

E

S

S

C

H

V

I

D

G

V

V

A

A

E

K

N

K

L

L

V

E

R

K

T

E

L

I

P

P

N

G

A

A

W

V

F

I

A

L

N

D

M
x
Q

Y

Y

Q

N

N

N

P

M

V

M

S

N

R

P

Q

E

V

A

H

H

Q

Y

D

F

G

G

T

T

G

G

P

R

E

E

I

I

F

Q

R

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

K
x
N

V

L

T

R

H

A

L

V

F

V

V

A

P

G

M
x
A

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

R

K

Y

Y

F

L

K

K

S

E

V

Q

N

N

P

D

E

K

V

I

Q

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

V

I

Y

L

G

A

D

Q

L

P

H

E

G

-

G

N

K

L

S

N

Q

K

G

T

T

D

P

I

S

T

A

D

F

Y

Q

K

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

V

T

P

S

Q

F

V

W

L

D

D

P

R

N

K

V

L

I

I

D

D

R

V

I

W

V

Y

Q

K

V

T

S

D

D

D

A

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

V

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

A

A

M

F

A

T

A

A

A

V

L

V

R

K

E

Y

G

C

E

E

A

D

Y

H

-

P

-

E

-

L

-

T

G

E

D

D

A

V

L

I

V

V

V

A

V

I

M

F

M
x
P

T
x
D

D

S

F

I

G

R

G

S

Y

Y

D

-

L

L

S

T

R

K

M

F

Y

V

N

D

D

D

D

E

W

W

M

L

R

K

Q

N

G

N

F

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K50 (≠ Y42), T78 (= T69), S79 (≠ F70), N81 (≠ T72), T82 (≠ S73), Q154 (≠ M145), A192 (≠ M178), G193 (≠ A179), T194 (≠ S180), G195 (= G181), T197 (≠ M183), G242 (≠ D229), S286 (≠ A273), P315 (≠ M298), D316 (≠ T299)

6c2qA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-l-serine intermediate (see paper)
31% identity, 70% coverage: 3:321/456 of query aligns to 9:337/345 of 6c2qA

query
sites
6c2qA

N

H

D

N

I

V

F

I

D

D

I

L

S

V

D

G

N

N

T

T

P

P

L

L

V

I

L

A

M

L

K

K

Q

K

L

L

T

P

R

K

Q

A

K

L

-

G

-

I

R

K

A

P

R

Q

F

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

S

N

P

P

C

G

F

G

S

S

H

I

Y
x
K

C

D

R

R

V

I

S

A

G

K

A

S

I

M

V

V

R

E

D

E

A

A

E

E

E

A

R

S

G

G

A

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T
|
T

F
x
S

G

G

T
x
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

A

G

V

A

S

I

R

K

G

G

Y

Y

K

R

V

T

L

I

A

T

A

L

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

V

L

L

R

K

A

A

L

L

G

G

A

A

E

E

L

I

V

I

L

R

T

T

P

P

S

T

E

A

A

A

L

A

P

W

G

D

D

S

L

P

Q

E

S

S

C

H

V

I

D

G

V

V

A

A

E

K

N

K

L

L

V

E

R

K

T

E

L

I

P

P

N

G

A

A

W

V

F

I

A

L

N

D

M
x
Q

Y

Y

Q

N

N

N

P

M

V

M

S

N

R

P

Q

E

V

A

H

H

Q

Y

D

F

G

G

T

T

G

G

P

R

E

E

I

I

F

Q

R

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

K
x
N

V

L

T

R

H

A

L

V

F

V

V

A

P

G

M
x
A

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

R

K

Y

Y

F

L

K

K

S

E

V

Q

N

N

P

D

E

K

V

I

Q

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

V

I

Y

L

G

A

D

Q

L

P

H

E

G

-

G

N

K

L

S

N

Q

K

G

T

T

D

P

I

S

T

A

D

F

Y

Q

K

L

V

E

E

D
x
G

I

I

G

G

A
x
Y

V

D

R

F

P

V

T

P

S

Q

F

V

W

L

D

D

P

R

N

K

V

L

I

I

D

D

R

V

I

W

V

Y

Q

K

V

T

S

D

D

D

A

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

V

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

A

A

M

F

A

T

A

A

A

V

L

V

R

K

E

Y

G

C

E

E

A

D

Y

H

-

P

-

E

-

L

-

T

G

E

D

D

A

V

L

I

V

V

V

A

V

I

M

F

M
x
P

T
x
D

D

S

F

I

G

R

G

S

Y

Y

D

-

L

L

S

T

R

K

M

F

Y

V

N

D

D

D

D

E

W

W

M

L

R

K

Q

N

G

N

F

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: K50 (≠ Y42), T78 (= T69), S79 (≠ F70), N81 (≠ T72), T82 (≠ S73), Q154 (≠ M145), A192 (≠ M178), G193 (≠ A179), T194 (≠ S180), G195 (= G181), T197 (≠ M183), G242 (≠ D229), Y245 (≠ A232), S286 (≠ A273), P315 (≠ M298), D316 (≠ T299)

6c2hA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the catalytic core (see paper)
31% identity, 70% coverage: 3:321/456 of query aligns to 9:337/345 of 6c2hA

query
sites
6c2hA

N

H

D

N

I

V

F

I

D

D

I

L

S

V

D

G

N

N

T

T

P

P

L

L

V

I

L

A

M

L

K

K

Q

K

L

L

T

P

R

K

Q

A

K

L

-

G

-

I

R

K

A

P

R

Q

F

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

S

N

P

P

C

G

F

G

S

S

H

I

Y
x
K

C

D

R

R

V

I

S

A

G

K

A

S

I

M

V

V

R

E

D

E

A

A

E

E

E

A

R

S

G

G

A

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T

T

F

S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

A

G

V

A

S

I

R

K

G

G

Y

Y

K

R

V

T

L

I

A

T

A

L

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

V

L

L

R

K

A

A

L

L

G

G

A

A

E

E

L

I

V

I

L

R

T

T

P

P

S

T

E

A

A

A

L

A

P

W

G

D

D

S

L

P

Q

E

S

S

C

H

V

I

D

G

V

V

A

A

E

K

N

K

L

L

V

E

R

K

T

E

L

I

P

P

N

G

A

A

W

V

F

I

A

L

N

D

M

Q

Y

Y

Q

N

N

N

P

M

V

M

S

N

R

P

Q

E

V

A

H

H

Q

Y

D

F

G

G

T

T

G

G

P

R

E

E

I

I

F

Q

R

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

K
x
N

V

L

T

R

H

A

L

V

F

V

V

A

P

G

M
x
A

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

R

K

Y

Y

F

L

K

K

S

E

V

Q

N

N

P

D

E

K

V

I

Q

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

V

I

Y

L

G

A

D

Q

L

P

H

E

G

-

G

N

K

L

S

N

Q

K

G

T

T

D

P

I

S

T

A

D

F

Y

Q

K

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

V

T

P

S

Q

F

V

W

L

D

D

P

R

N

K

V

L

I

I

D

D

R

V

I

W

V

Y

Q

K

V

T

S

D

D

D

A

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

V

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

A

A

M

F

A

T

A

A

A

V

L

V

R

K

E

Y

G

C

E

E

A

D

Y

H

-

P

-

E

-

L

-

T

G

E

D

D

A

V

L

I

V

V

V

A

V

I

M

F

M
x
P

T
x
D

D

S

F

I

G

R

G

S

Y

Y

D

-

L

L

S

T

R

K

M

F

Y

V

N

D

D

D

D

E

W

W

M

L

R

K

Q

N

G

N

F

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
binding pyridoxal-5'-phosphate: K50 (≠ Y42), N81 (≠ T72), A192 (≠ M178), G193 (≠ A179), T194 (≠ S180), G195 (= G181), T197 (≠ M183), G242 (≠ D229), S286 (≠ A273), P315 (≠ M298), D316 (≠ T299)

6c4pA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the pmp complex (see paper)
31% identity, 70% coverage: 3:321/456 of query aligns to 8:336/344 of 6c4pA

query
sites
6c4pA

N

H

D

N

I

V

F

I

D

D

I

L

S

V

D

G

N

N

T

T

P

P

L

L

V

I

L

A

M

L

K

K

Q

K

L

L

T

P

R

K

Q

A

K

L

-

G

-

I

R

K

A

P

R

Q

F

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

S

N

P

P

C

G

F

G

S

S

H

I

Y
x
K

C

D

R

R

V

I

S

A

G

K

A

S

I

M

V

V

R

E

D

E

A

A

E

E

E

A

R

S

G

G

A

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T

T

F

S

G

G

T
x
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

A

G

V

A

S

I

R

K

G

G

Y

Y

K

R

V

T

L

I

A

T

A

L

P

P

E

E

S

K

I

M

C

S

R

N

E

E

K

K

Q

V

E

S

V

V

L

L

R

K

A

A

L

L

G

G

A

A

E

E

L

I

V

I

L

R

T

T

P

P

S

T

E

A

A

A

L

A

P

W

G

D

D

S

L

P

Q

E

S

S

C

H

V

I

D

G

V

V

A

A

E

K

N

K

L

L

V

E

R

K

T

E

L

I

P

P

N

G

A

A

W

V

F

I

A

L

N

D

M

Q

Y

Y

Q

N

N

N

P

M

V

M

S

N

R

P

Q

E

V

A

H

H

Q

Y

D

F

G

G

T

T

G

G

P

R

E

E

I

I

F

Q

R

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

K
x
N

V

L

T

R

H

A

L

V

F

V

V

A

P

G

M
x
A

A
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

R

K

Y

Y

F

L

K

K

S

E

V

Q

N

N

P

D

E

K

V

I

Q

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

V

I

Y

L

G

A

D

Q

L

P

H

E

G

-

G

N

K

L

S

N

Q

K

G

T

T

D

P

I

S

T

A

D

F

Y

Q

K

L

V

E

E

D
x
G

I

I

G

G

A

Y

V

D

R

F

P

V

T

P

S

Q

F

V

W

L

D

D

P

R

N

K

V

L

I

I

D

D

R

V

I

W

V

Y

Q

K

V

T

S

D

D

D

A

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

V

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

A

A

M

F

A

T

A

A

A

V

L

V

R

K

E

Y

G

C

E

E

A

D

Y

H

-

P

-

E

-

L

-

T

G

E

D

D

A

V

L

I

V

V

V

A

V

I

M

F

M
x
P

T
x
D

D

S

F

I

G

R

G

S

Y

Y

D

-

L

L

S

T

R

K

M

F

Y

V

N

D

D

D

D

E

W

W

M

L

R

K

Q

N

G

N

F

L

Y

W

binding calcium ion: N179 (vs. gap), D182 (≠ G169), N183 (≠ K170)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: K49 (≠ Y42), N80 (≠ T72), A191 (≠ M178), G192 (≠ A179), T193 (≠ S180), G194 (= G181), T196 (≠ M183), G241 (≠ D229), S285 (≠ A273), P314 (≠ M298), D315 (≠ T299)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
33% identity, 68% coverage: 11:318/456 of query aligns to 14:320/323 of P0ABK5

query
sites
P0ABK5

N

H

T

T

P

P

L

L

V

V

L

-

M

-

K

-

Q

R

L

L

T

N

R

R

Q

I

K

G

R

N

A

G

R

R

F

I

M

L

A

A

K

K

L

V

E

E

Y

S

M

R

S

N

P

P

C

S

F

F

S

S

H

V

Y
x
K

C

C

R

R

V

I

S

G

G

A

A

N

I

M

V

I

R

W

D

D

A

A

E

E

E

K

R

R

G

G

A

V

I

L

H

K

P

P

G

G

M

V

T

E

L

L

V

V

D

E

W

P

T

T

F

S

G

G

T

N

S

T

G

G

I

I

A

A

L

L

A

A

M

Y

A

V

A

A

V

A

S

A

R

R

G

G

Y

Y

K

K

V

L

L

T

L

L

A

T

A

M

P

P

E

E

S

T

I

M

C

S

R

I

E

E

K

R

Q

R

E

K

V

L

L

L

R

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

-

S

-

E

E

A

G

L

A

P

K

G

G

D

-

L

M

Q

K

S

G

C

A

V

I

D

Q

V

K

A

A

E

E

N

E

L

I

V

V

R

A

T

S

L

N

P

P

N

E

A

K

W

Y

-

L

F

L

A

L

N

Q

M

Q

Y

F

Q

S

N

N

P

P

V

A

S

N

R

P

Q

E

V

I

H

H

Q

E

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K42 (≠ Y42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_010933579.1 NCBI__GCF_000006985.1:WP_010933579.1
MTNDIFDISDNTPLVLMKQLTRQKRARFMAKLEYMSPCFSHYCRVSGAIVRDAEERGAIH
PGMTLVDWTFGTSGIALAMAAVSRGYKVLLAAPESICREKQEVLRALGAELVLTPSEALP
GDLQSCVDVAENLVRTLPNAWFANMYQNPVSRQVHQDGTGPEIFRQTEGKVTHLFVPMAS
GAMISGIGRYFKSVNPEVQIIGVEPEGSVYGDLHGGKSQGTPSAFQLEDIGAVRPTSFWD
PNVIDRIVQVSDADAFNCGRELLRAEAVFAGGASGAAMAAALREGEAYGDDALVVVMMTD
FGGYDLSRMYNDDWMRRQGFYRKSKTALDQITADDILQRKAHKDLIFAQPEQTLAEVFET
MKQNDVSQMPIVSFGAPIGSISENRILSILIENDSAMNAKVVAFMEKPFPVCQPDATISE
LSEKLQTNASGVLISLSDGRLQLINKSDLIDVLTRK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory