SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010936234.1 NCBI__GCF_000011905.1:WP_010936234.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

5ygeA Arga complexed with acecoa and glutamate (see paper)
32% identity, 85% coverage: 3:136/158 of query aligns to 6:140/169 of 5ygeA

query
sites
5ygeA

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binding acetyl coenzyme *a: Y25 (≠ F22), I73 (≠ V69), V76 (≠ L72), A77 (= A73), V78 (= V74), T83 (≠ H79), G84 (≠ R80), H85 (≠ Q81), G86 (= G82), G88 (= G84), H89 (≠ E85), T111 (= T107), E113 (≠ K109), F117 (= F113)
binding glutamic acid: K33 (≠ R31), E44 (≠ D42), H64 (= H60), R74 (≠ K70)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
32% identity, 85% coverage: 3:136/158 of query aligns to 5:139/168 of 6addA

query
sites
6addA

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binding coenzyme a: Y24 (≠ F22), I28 (≠ Q27), V75 (≠ L72), A76 (= A73), V77 (= V74), T82 (≠ H79), G83 (≠ R80), H84 (≠ Q81), G85 (= G82), G87 (= G84), H88 (≠ E85), T110 (= T107), E112 (≠ K109), F115 (= F112)
binding n~2~-acetyl-l-glutamine: K27 (≠ H25), I28 (≠ Q27), L30 (= L29), T74 (≠ S71), L109 (= L106)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
32% identity, 85% coverage: 3:136/158 of query aligns to 5:139/168 of 5yo2A

query
sites
5yo2A

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binding acetyl coenzyme *a: Y24 (≠ F22), I28 (≠ Q27), I72 (≠ V69), R73 (≠ K70), T74 (≠ S71), V75 (≠ L72), V77 (= V74), T82 (≠ H79), G83 (≠ R80), H84 (≠ Q81), G85 (= G82), G87 (= G84), H88 (≠ E85), T110 (= T107), E112 (≠ K109), F115 (= F112), F116 (= F113)
binding arginine: G26 (≠ D24), L29 (≠ M28), L30 (= L29), R73 (≠ K70), L109 (= L106)

Sites not aligning to the query:

binding arginine: 147

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
28% identity, 79% coverage: 3:127/158 of query aligns to 276:401/424 of 4i49A

query
sites
4i49A

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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ Q27), L301 (≠ M28), L302 (= L29), R304 (= R31), A343 (≠ K70), C344 (≠ S71), L345 (= L72), A346 (= A73), V347 (= V74), Q352 (≠ H79), D353 (≠ R80), G355 (= G82), G357 (= G84), E358 (= E85), L379 (= L106), S380 (≠ T107), T383 (≠ P110), W386 (≠ F112), F387 (= F113)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
28% identity, 79% coverage: 3:127/158 of query aligns to 276:401/424 of 3d2mA

query
sites
3d2mA

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binding coenzyme a: L345 (= L72), V347 (= V74), Q352 (≠ H79), D353 (≠ R80), G355 (= G82), G357 (= G84), E358 (= E85), S380 (≠ T107), T383 (≠ P110), W386 (≠ F112)
binding glutamic acid: L302 (= L29), R304 (= R31), A343 (≠ K70), C344 (≠ S71), L379 (= L106)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
28% identity, 79% coverage: 3:127/158 of query aligns to 276:401/424 of 3b8gA

query
sites
3b8gA

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binding coenzyme a: I300 (≠ Q27), L345 (= L72), V347 (= V74), Q352 (≠ H79), D353 (≠ R80), G355 (= G82), Y356 (≠ L83), G357 (= G84), E358 (= E85), S380 (≠ T107), T383 (≠ P110), E385 (vs. gap), W386 (≠ F112)
binding n-acetyl-l-glutamate: L302 (= L29), R304 (= R31), A343 (≠ K70), C344 (≠ S71), L379 (= L106)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
28% identity, 79% coverage: 3:127/158 of query aligns to 276:401/424 of 2r8vA

query
sites
2r8vA

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binding acetyl coenzyme *a: I300 (≠ Q27), L301 (≠ M28), L345 (= L72), V347 (= V74), Q352 (≠ H79), D353 (≠ R80), G355 (= G82), G357 (= G84), E358 (= E85), T383 (≠ P110), E385 (vs. gap), W386 (≠ F112), F387 (= F113)

Sites not aligning to the query:

active site: 26

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
29% identity, 63% coverage: 28:127/158 of query aligns to 305:405/424 of 3d2pA

query
sites
3d2pA

M

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A

A

L

L

H

K

-

T

I

F

S

A

W

E

A

A

D

D

L

C

A

G

E

E

V

I

K

A

S

C

L
|
L

A

A

V
|
V

S

S

E

P

E

Q

R

A

H
x
Q

R
x
D

Q

G

G
|
G

L

Y

G
|
G

E
|
E

M

R

V

L

V

L

K

A

A

H

C

I

L

I

E

D

D

K

A

A

K

R

S

G

L

I

Q

G

I

I

P

S

T

R

V

L

F

F

C

A

L

L

T

S

Y

T

K

N

P

T

A

G

-
x
E

F
x
W

F

F

A

A

K

E

C

R

G

G

F

F

H

Q

Q

T

V

A

E

S

K

E

N

D

A

E

L

L

P

P

binding coenzyme a: L349 (= L72), V351 (= V74), Q356 (≠ H79), D357 (≠ R80), G359 (= G82), G361 (= G84), E362 (= E85), E389 (vs. gap), W390 (≠ F112)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276
binding coenzyme a: 303

6yugA Crystal structure of c. Parvum gna1 in complex with acetyl-coa and glucose 6p. (see paper)
24% identity, 61% coverage: 2:97/158 of query aligns to 4:106/143 of 6yugA

query
sites
6yugA

Y

F

T

E

I

V

D

R

K

K

A

A

T

T

I

I

K

D

D

D

A

Y

T

F

T

E

I

L

H

R

K

N

L

L

V

I

N

C

N

D

F

V

A

T

D
x
R

H

C

G

T

Q

E

M

T

L

L

A

S

R

R

S

E

L

Q

A

A

E

E

-

E

-

R

-

F

I

R

Y

Y

E

N

N

T

L

Y

R

H

D

P

F

Y

Y

C

V

L

I

V

R

D

G

T

D

E

N

N

G

G

E

R

V

I

I

V

A

G

C

Y

A

A

A

G

L

F

-

Y

-

I

-

I

-

P

H

H

I

L

S

G

W

R

A

K

D

N

L

D

A

S

E

R

V

I

K
x
E

S
x
H

L
x
V

A
x
I

V
x
I

S

S

E

K

E

E

R

Y

H
x
R

R
x
N

Q
x
R

G
|
G

L

L

G
|
G

E
x
R

M

L

V

L

V

C

K

K

A

Q

C

I

L

I

E

E

D

D

A

A

K

K

S

N

binding acetyl coenzyme *a: H80 (≠ S71), V81 (≠ L72), I82 (≠ A73), I83 (≠ V74), R88 (≠ H79), N89 (≠ R80), R90 (≠ Q81), G91 (= G82), G93 (= G84), R94 (≠ E85)
binding 6-O-phosphono-alpha-D-glucopyranose: R26 (≠ D24), E79 (≠ K70), H80 (≠ S71)

Sites not aligning to the query:

Query Sequence

>WP_010936234.1 NCBI__GCF_000011905.1:WP_010936234.1
MYTIDKATIKDATTIHKLVNNFADHGQMLARSLAEIYENLRDFYVIRGDNGEVIACAALH
ISWADLAEVKSLAVSEERHRQGLGEMVVKACLEDAKSLQIPTVFCLTYKPAFFAKCGFHQ
VEKNALPHKIWGECYRCPKFPNCDETAMMLSLTEANIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory