SitesBLAST
Comparing WP_010959858.1 NCBI__GCF_000008325.1:WP_010959858.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
38% identity, 98% coverage: 4:458/466 of query aligns to 3:457/471 of P04805
- C98 (= C103) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C105) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C130) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (= H132) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ R139) mutation to Q: No change in activity or in zinc content.
- H131 (≠ R141) mutation to Q: No change in activity or in zinc content.
- H132 (= H142) mutation to Q: No change in activity or in zinc content.
- C138 (≠ A148) mutation to S: No change in activity or in zinc content.
- S239 (= S249) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
38% identity, 98% coverage: 4:458/466 of query aligns to 3:457/468 of 8i9iA
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
34% identity, 97% coverage: 3:454/466 of query aligns to 1:473/484 of 2cfoA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
29% identity, 98% coverage: 3:458/466 of query aligns to 1:475/485 of 4griB
- active site: S9 (= S11), K253 (= K250)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E39), Y194 (≠ F191), R212 (= R209), W216 (≠ H213)
- binding zinc ion: C105 (= C103), C107 (= C105), Y128 (= Y126), C132 (= C130)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
34% identity, 97% coverage: 3:454/466 of query aligns to 2:474/485 of Q8DLI5
- R6 (= R7) binding L-glutamate
- Y192 (≠ F191) binding L-glutamate
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
40% identity, 70% coverage: 4:329/466 of query aligns to 3:304/380 of 4g6zA
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
38% identity, 69% coverage: 1:322/466 of query aligns to 1:322/488 of 8vc5A
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 103:555/564 of 3al0C
- active site: S110 (= S11), K335 (= K250)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (= T23), E142 (= E39), Y276 (≠ F191), R294 (= R209), G295 (= G210), D297 (= D212), H298 (= H213), L324 (= L239), I325 (= I240), L333 (= L248)
- binding : T144 (= T41), D145 (= D42), R148 (= R45), Y208 (≠ F104), P213 (≠ V112), K252 (≠ R167), M255 (≠ Q170), I266 (= I181), K269 (≠ R184), S270 (≠ A185), Y276 (≠ F191), D297 (= D212), H298 (= H213), L299 (= L214), S300 (≠ A215), N301 (= N216), K304 (≠ R219), R330 (≠ G245), P332 (= P247), G363 (= G278), W364 (≠ H279), R365 (≠ H280), E370 (= E285), S387 (≠ G302), K389 (≠ S304), V391 (≠ A306), I392 (≠ R307), K397 (≠ H312), W400 (≠ H315), R407 (= R322), E446 (≠ S355), K447 (≠ N356), Q453 (≠ E362), I457 (≠ W366), R509 (≠ A412), K520 (= K423), Q524 (≠ L427), R527 (= R430), V535 (≠ D438), T536 (≠ G439), G538 (≠ E441), L539 (= L442)
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
33% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 1g59A
- binding : D44 (= D42), R45 (≠ L43), A46 (≠ E44), R47 (= R45), P109 (= P107), V145 (≠ T149), R163 (= R167), V166 (≠ Q170), E172 (≠ D176), V177 (≠ I181), K180 (≠ R184), S181 (≠ A185), D182 (= D186), E207 (= E211), E208 (≠ D212), R237 (≠ V241), K241 (≠ G245), T242 (≠ A246), K243 (≠ P247), M273 (≠ L277), G274 (= G278), E282 (= E285), S299 (≠ G302), L300 (≠ R303), P303 (≠ A306), V304 (≠ R307), K309 (≠ H312), W312 (≠ H315), R319 (= R322), P357 (≠ S355), R358 (≠ N356), R417 (≠ A412), K426 (= K421), L427 (≠ G422), Q432 (≠ L427), R435 (= R430), L442 (= L437), E443 (≠ D438), T444 (≠ G439), P445 (= P440), G446 (≠ E441), L447 (= L442), F448 (≠ A443)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 2cv2A
- active site: K246 (= K250)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ T23), E41 (= E39), Y187 (≠ F191), R205 (= R209), A206 (≠ G210), E208 (≠ D212), W209 (≠ H213), L235 (= L239), L236 (≠ I240)
- binding : S9 (= S11), T43 (= T41), D44 (= D42), R47 (= R45), V145 (≠ T149), R163 (= R167), Y168 (≠ F172), E172 (≠ D176), V177 (≠ I181), K180 (≠ R184), S181 (≠ A185), Y187 (≠ F191), E207 (= E211), E208 (≠ D212), W209 (≠ H213), V211 (≠ A215), R237 (≠ V241), K241 (≠ G245), L272 (≠ R276), M273 (≠ L277), G274 (= G278), E282 (= E285), S299 (≠ G302), P303 (≠ A306), V304 (≠ R307), K309 (≠ H312), W312 (≠ H315), R319 (= R322), P357 (≠ S355), R358 (≠ N356), R417 (≠ A412), Q432 (≠ L427), R435 (= R430), L442 (= L437), E443 (≠ D438), T444 (≠ G439), G446 (≠ E441), L447 (= L442), F448 (≠ A443)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 2cv1A
- active site: K246 (= K250)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ N20), I21 (≠ T23), R47 (= R45), A206 (≠ G210), W209 (≠ H213), L235 (= L239), L236 (≠ I240)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E39), Y187 (≠ F191), R205 (= R209), W209 (≠ H213)
- binding : S9 (= S11), E41 (= E39), T43 (= T41), D44 (= D42), R47 (= R45), V145 (≠ T149), R163 (= R167), V166 (≠ Q170), E172 (≠ D176), V177 (≠ I181), K180 (≠ R184), S181 (≠ A185), Y187 (≠ F191), E207 (= E211), E208 (≠ D212), W209 (≠ H213), V211 (≠ A215), R237 (≠ V241), K241 (≠ G245), K243 (≠ P247), M273 (≠ L277), G274 (= G278), S276 (≠ H280), E282 (= E285), S299 (≠ G302), P303 (≠ A306), V304 (≠ R307), K309 (≠ H312), W312 (≠ H315), R319 (= R322), P357 (≠ S355), R358 (≠ N356), R417 (≠ A412), L427 (≠ G422), Q432 (≠ L427), R435 (= R430), L442 (= L437), E443 (≠ D438), T444 (≠ G439), G446 (≠ E441), L447 (= L442), F448 (≠ A443)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 1n78A
- active site: K246 (= K250)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ N20), I21 (≠ T23), E41 (= E39), Y187 (≠ F191), N191 (= N195), R205 (= R209), A206 (≠ G210), E208 (≠ D212), W209 (≠ H213), L235 (= L239), L236 (≠ I240)
- binding : S9 (= S11), T43 (= T41), D44 (= D42), R47 (= R45), V145 (≠ T149), R163 (= R167), V166 (≠ Q170), Y168 (≠ F172), E172 (≠ D176), V177 (≠ I181), K180 (≠ R184), S181 (≠ A185), Y187 (≠ F191), E207 (= E211), E208 (≠ D212), W209 (≠ H213), L210 (= L214), V211 (≠ A215), R237 (≠ V241), K241 (≠ G245), M273 (≠ L277), G274 (= G278), E282 (= E285), R297 (= R300), P303 (≠ A306), V304 (≠ R307), K309 (≠ H312), W312 (≠ H315), R319 (= R322), P357 (≠ S355), R358 (≠ N356), R417 (≠ A412), L427 (≠ G422), Q432 (≠ L427), R435 (= R430), L442 (= L437), E443 (≠ D438), T444 (≠ G439), G446 (≠ E441), L447 (= L442), F448 (≠ A443)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of 1j09A
- active site: K246 (= K250)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D212), L235 (= L239), L236 (≠ I240), K243 (≠ P247), I244 (≠ L248), S245 (= S249), K246 (= K250), R247 (= R251)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E39), Y187 (≠ F191), N191 (= N195), R205 (= R209), W209 (≠ H213)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 98% coverage: 4:458/466 of query aligns to 2:463/468 of P27000
- R358 (≠ N356) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
29% identity, 98% coverage: 4:458/466 of query aligns to 3:494/502 of 6brlA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
31% identity, 53% coverage: 7:252/466 of query aligns to 19:243/308 of P27305
- E55 (= E39) binding L-glutamate
- Y182 (≠ F191) binding L-glutamate
- R200 (= R209) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
31% identity, 53% coverage: 7:251/466 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S11), K229 (= K250)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (= E39), Y170 (≠ F191), R188 (= R209), L192 (≠ H213)
- binding zinc ion: C99 (= C103), C101 (= C105), Y113 (= Y126), C117 (= C130)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
26% identity, 59% coverage: 4:278/466 of query aligns to 11:278/455 of 3aiiA
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 51% coverage: 1:237/466 of query aligns to 199:425/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_010959858.1 NCBI__GCF_000008325.1:WP_010959858.1
MSAIKTRFAPSPTGLIHLGNARTALFSALGGDVFVLRIEDTDLERSRAEFVAELMNDLRW
LGLDWQEGPRGAEPDPDWYQSRRGEIYATYYRLLEEKGLAYPCFCTPLELEVSRKVQLGS
GRPPRYSGRCAHLPADEVRRRHEEGLAATLRFRVPKDRVVEFEDEVRGPQRFAGEDIGDF
IIRRADGSPAFFFCNAIDDALMGITRVLRGEDHLANTPRQLMILAALDLPRPRYAHISLI
VGDDGAPLSKRNGSRSIKQLREEGYFPEAVVNMLARLGHHYDSAELLGLPALRAGFDIRR
LGRSPARFDVSHLDHWQGLAVRGAADDTLWHWLHTETRAVVPDAHRAGFLDLVRSNCLFP
KEADAWARILFTDELELAADIAAVAQAAGEAFYLAAIDAATESPDDFAAFLAGLKRRSGA
KGKHLFLPLRAALTGSLDGPELAKIYQMLDKPRLHRRLAEFTYESE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory