SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010960694.1 NCBI__GCF_000008325.1:WP_010960694.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
31% identity, 99% coverage: 1:358/362 of query aligns to 1:376/386 of P0A9J8

query
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P0A9J8

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R11 (= R11) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R28) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K39) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E54) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ E89) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
35% identity, 73% coverage: 94:357/362 of query aligns to 4:273/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T262), F171 (= F264)
binding phenylalanine: N204 (= N288), E205 (≠ D289), L209 (= L293), D222 (≠ S306), L223 (≠ M307), T224 (= T308), K225 (= K309), S228 (= S312), Y238 (= Y322), F240 (= F324)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
35% identity, 74% coverage: 92:360/362 of query aligns to 1:277/306 of 3mwbA

query
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3mwbA

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active site: T176 (= T262), F178 (= F264)
binding phenylalanine: D205 (= D289), H206 (vs. gap), L210 (= L293), N223 (≠ S306), L224 (≠ M307), S225 (≠ T308), R226 (≠ K309), S229 (= S312), F241 (= F324)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
35% identity, 74% coverage: 92:360/362 of query aligns to 1:274/303 of 3mwbB

query
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3mwbB

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active site: T176 (= T262), F178 (= F264)
binding magnesium ion: A155 (= A241), Q158 (≠ L244), L161 (= L247)
binding phenylalanine: D205 (= D289), H206 (vs. gap), L210 (= L293), N223 (≠ S306), L224 (≠ M307), S225 (≠ T308), R226 (≠ K309), I227 (= I310), Y236 (= Y322), F238 (= F324)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
32% identity, 73% coverage: 94:357/362 of query aligns to 9:276/282 of 6vh5D

query
sites
6vh5D

V

I

A

S

F

F

L

Q

G

G

P

E

E

A

G

G

T

A

F

N

T

S

Q

D

Q

T

A

A

A

C

Y

R

R

N

H

M

F

F

G

P

H

D

A

-

I

M

Q

E

A

P

V

L

P

P

M

C

P

P

A

T

I

F

D

E

E

D

I

A

F

F

R

N

A

A

V

V

E

E

S

T

G

G

A

A

C

A

H

D

Y

L

G

A

V

M

V

I

P

P

V

I

E

E

N

N

S

T

T

L

E

A

G

G

V

R

I

V

T

A

H

D

T

I

L

H

D

Y

S

L

F

L

V

P

R

L

F

A

S

D

L

M

I

H

I

I

A

V

G

G

E

E

V

Y

Q

F

L

L

R

P

I

I

H

H

H

F

N

Q

L

L

L

M

C

V

R

L

T

P

P

G

T

V

A

R

L

R

T

E

E

E

L

I

T

K

E

T

V

V

F

H

S

S

H

H

P

I

Q

H

S

A

L

L

A

G

Q

Q

C

C

R

R

G

N

W

V

L

I

D

R

R

Q

F

-

L

-

P

N

G

G

V

W

R

K

R

G

T

V

P

I

L

A

G

G

S

D

N

T

A

A

E

G

A

A

R

R

R

L

A

V

A

A

E

D

T

V

A

K

-

D

-

R

G

S

T

M

A

A

I

L

A

A

G

P

E

R

V

L

A

A

G

D

L

L

Y

Y

G

G

L

L

E

D

I

I

L

L

N

E

R

E

N

N

I

V

E

E

D

D

E

S

P

E

D

N

N

N

T

V

T

T

R

R

F

F

L

V

V

V

I

L

G

S

G

K

Q

N

P

K

-

Q

-

W

-

A

V

A

G

R

P

P

T

E

G

N

H

D

D

E

K

R

-

I

-

V

T

T

S

T

L

F

L

V

L

F

S

R

T

V

R

R

N
|
N

D
x
V

P

P

G

A

A

A

L
|
L

F

Y

R

K

L

A

I

L

E

G

P

G

F

F

A

A

R

T

L

N

G

G

I

V

S
x
N

M
|
M

T
|
T

K
|
K

I

L

E

E

S
|
S

R

Y

P

Q

S

L

R

G

G

R

M

F

W

I

D

A

Y
x
T

F

Q

F
|
F

F

Y

I

A

D

D

V

I

E

E

G

G

H

H

Q

P

A

E

D

E

P

R

T

S

L

V

A

Q

Q

L

A

A

L

L

A

E

E

E

V

L

R

R

E

F

H

F

C

T

C

K

M

E

M

V

R

R

I

I

L

L

G

G

S

V

Y

Y

binding phenylalanine: N207 (= N288), V208 (≠ D289), L212 (= L293), N225 (≠ S306), M226 (= M307), T227 (= T308), K228 (= K309), S231 (= S312), T241 (≠ Y322), F243 (= F324)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
32% identity, 73% coverage: 94:358/362 of query aligns to 4:270/278 of 7am0B

query
sites
7am0B

V

I

A

A

F

F

L

Q

G

G

P

R

E

P

G

G

T

A

F

Y

T

S

Q

D

Q

L

A

A

A

C

Y

-

R

R

H

Q

F

A

G

R

H

P

A

G

I

W

Q

T

A

T

V

L

P

P

M

C

P

Q

A

T

I

F

D

A

E

Q

I

T

F

I

R

A

A

A

V

V

E

H

S

D

G

G

A

R

C

A

H

E

Y

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

T

L

E

A

G

G

V

R

I

V

T

P

H

D

T

I

L

H

D

A

S

L

F

L

V

P

R

E

F

A

S

G

L

L

I

F

I

I

A

V

G

G

E

E

V

H

Q

F

L

Q

R

R

I

V

H

E

H

H

N

C

L

L

C

G

R

I

T

P

P

G

T

S

A

T

L

L

T

A

E

D

L

A

T

R

E

R

V

I

F

H

S

T

H

H

P

P

Q

V

S

A

L

M

A

A

Q

Q

C

V

R

R

G

G

W

I

L

I

D

T

R

E

F

L

-

G

L

L

P

D

G

P

V

V

R

V

R

E

T

F

P

D

L

T

G

A

S

G

N

A

A

A

E

E

A

M

A

V

R

R

R

E

A

W

A

G

E

R

T

K

A

E

G

D

T

V

A

A

A

-

I

V

A

A

G

S

E

A

V

L

A

A

G

E

L

L

Y

N

G

G

L

L

E

E

I

I

L

L

N

R

R

R

N

N

I

V

E

E

D

D

E

A

P

T

D

H

N

N

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

G

R

Q

R

P

P

-

A

-

T

V

L

G

P

P

P

T

P

G

G

H

P

D

G

-

F

K

M

T

T

S

T

L

L

L

F

S

R

T

V

R
x
N

N
|
N

D
x
Q

P
|
P

G

G

A

A

L
|
L

F

Y

R

K

L

A

I

L

E

G

P

G

F

L

A

A

R

T

L

A

G

G

I

V

S
x
N

M
|
M

T
|
T

K
x
R

I

L

E

E

S

S

R

Y

P

M

S

L

R

E

R

G

G

S

M

F

W

S

D

A

Y
x
T

F

Q

F
|
F

F

L

I

M

D

D

V

V

E

E

G

G

H

H

Q

P

A

E

D

A

P

P

T

P

L

L

A

A

Q

R

A

A

L

L

A

D

E

E

V

L

R

S

E

F

H

F

C

S

C

E

M

Q

R

E

I

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: N199 (≠ R287), N200 (= N288), Q201 (≠ D289), P202 (= P290), L205 (= L293), N218 (≠ S306), M219 (= M307), T220 (= T308), R221 (≠ K309), T234 (≠ Y322), F236 (= F324)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
28% identity, 73% coverage: 96:359/362 of query aligns to 8:285/326 of 3luyA

query
sites
3luyA

F

Y

L

L

G

G

P

P

E

Q

G

G

T

T

F

F

T

T

Q

H

Q

Q

A

A

Y

V

-

N

-

A

-

Q

-

E

-

L

-

A

R

R

H

F

F

E

G

P

H

Q

A

G

I

F

Q

D

A

L

V

M

P

P

M

M

P

D

A

D

I

V

D

P

E

Q

I

I

F

L

R

D

A

A

V

A

E

Q

S

H

G

G

A

D

C

G

H

-

Y

W

G

G

V

I

V

V

P

A

V

W

E

E

N

N

S

N

T

V

E

E

G

G

V

Y

I

V

T

V

H

P

T

N

L

L

D

D

S

A

F

L

V

I

R

D

F

A

S

K

L

D

I

L

I

V

A

G

-

F

G

A

E

R

V

V

Q

G

L

V

R

N

I

V

H

E

H

F

N

D

L

A

L

Y

C

V

R

A

T

Q

P

G

T

A

A

D

L

P

T

A

E

E

L

A

T

R

E

I

V

A

F

T

S

A

H

H

P

P

Q

H

S

G

L

L

A

A

Q

Q

C

C

R

K

G

-

W

-

L

-

D

-

R

R

F

F

L

I

P

A

G

E

V

H

R

R

R

L

T

S

-

T

-

Q

P

P

L

A

G

T

S

S

N

N

A

A

E

A

A

A

A

C

R

R

R

D

-

L

-

I

A

P

A

G

E

E

T

I

A

A

G

F

T

G

A

P

A

A

I

I

A

C

G

G

E

E

V

-

A

-

G

-

L

L

Y

Y

G

D

L

I

E

T

I

R

L

I

N

G

R

T

N

A

I

I

E

Q

D

D

E

Y

P

Q

D

G

N

A

T

A

T
|
T

R

D

F

F

L

L

V

V

I

L

G

S

-

P

-

R

-

A

-

E

-

V

-

A

-

R

-

L

G

A

Q

K

P

P

V

R

G

A

P

E

T

A

G

N

H

V

D

E

K

Y

T

E

S

S

L

V

L

L

-

T

-

L

L

I

S

P

T

L

R

V

N

T

D
x
G

P

P

G

G

A

V

L
|
L

F

A

R

N

L

L

I

L

E

D

P

V

F

F

A

R

R

D

L

A

G

G

I

L

S

N

M

M

T

T

K

S

I

F

E

I

S

S

R

R

P

P

S

I

R

K

R

G

G

R

M

T

W

G

D

T

Y
|
Y

F

S

F
|
F

F

I

I

V

D

T

V

L

E

D

G

A

H

A

Q

P

A

W

D

E

P

E

T

R

L

F

A

R

Q

D

A

A

L

L

A

V

E

E

V

I

R

A

E

E

H

H

C

G

C

D

M

W

M

A

R

K

I

T

L

L

G

A

S

V

Y

Y

P

P

R

R

active site: T177 (= T262)
binding 3-phenylpyruvic acid: G215 (≠ D289), L219 (= L293), Y248 (= Y322), F250 (= F324)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
38% identity, 27% coverage: 260:358/362 of query aligns to 85:186/194 of 7alzA

query
sites
7alzA

N

N

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

G

R

Q

R

P

P

-

A

-

T

V

L

G

P

P

P

T

P

G

G

H

P

D

G

-

F

K

M

T

T

S

T

L

L

L

F

S

R

T

V

R

N

N

N

D
x
Q

P

P

G

G

A

A

L
|
L

F

Y

R

K

L

A

I

L

E

G

P

G

F

L

A

A

R

T

L

A

G

G

I

V

S
x
N

M
|
M

T
|
T

K
x
R

I

L

E

E

S

S

R

Y

P

M

S

L

R

E

R

G

G

S

M

F

W

S

D

A

Y
x
T

F

Q

F
|
F

F

L

I

M

D

D

V

V

E

E

G

G

H

H

Q

P

A

E

D

A

P

P

T

P

L

L

A

A

Q

R

A

A

L

L

A

D

E

E

V

L

R

S

E

F

H

F

C

S

C

E

M

Q

R

E

I

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: Q117 (≠ D289), L121 (= L293), N134 (≠ S306), M135 (= M307), T136 (= T308), R137 (≠ K309), T150 (≠ Y322), F152 (= F324)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
36% identity, 23% coverage: 7:90/362 of query aligns to 4:85/352 of 5j6fA

query
sites
5j6fA

L

L

A

D

E

E

L

L

R

R

K

A

R

R

I

V

D

D

E

E

L

I

D

N

D

L

R

Q

V

L

L

L

E

K

L

L

L

I

N

N

Q

E

R
|
R

A

G

R

R

C

L

A

V

Q

Q

R

E

V
x
I

A

G

D

K

I

I

K
|
K

V

E

A

A

A

Q

G

G

E

T

T

H

D

R

C

-

F

-

Y
|
Y

R
x
D

P

P

E

V

R
|
R

E

E

A

R

E

K

I
x
M

L

L

Q

D

R

L

L

I

T

S

A

E

H

H

N

N

P

D

G

G

P

P

L

F

G

E

R

T

E

S

A

T

V

L

V

Q

R

H

F

I

F

F

R

K

E

E

V

I

M

F

S

K

E

A

C

A

L

L

A

E

L

L

E

Q

K

E

binding prephenic acid: R25 (= R28), I32 (≠ V35), K36 (= K39), Y44 (= Y49), D45 (≠ R50), R48 (= R53), M52 (≠ I57)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
35% identity, 23% coverage: 9:90/362 of query aligns to 1:75/345 of 3nvtA

query
sites
3nvtA

E

E

L

L

R

R

K

T

R

Q

I

V

D

D

E

Q

L

L

D

N

D

I

R

D

V

L

L

L

E

E

L

L

L

I

N

S

Q

K

R

R

A

A

R

N

C

L

A

V

Q

Q

R

E

V

I

A

G

D

K

I

I

K

K

V

-

A

-

G

G

E

R

T

F

D

D

C

-

F

-

Y

-

R

-

P

P

E

L

R

R

E

E

A

R

E

E

I

M

L

L

Q

N

R

T

L

I

T

L

A

A

H

A

N

N

P

E

G

G

P

P

L

F

G

E

R

D

E

S

A

T

V

V

V

Q

R

K

F

L

F

F

R

K

E

E

V

I

M

F

S

K

E

A

C

G

L

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
32% identity, 22% coverage: 10:90/362 of query aligns to 1:74/343 of 3tfcA

query
sites
3tfcA

L

L

R

R

K

T

R

Q

I

V

D

D

E

Q

L

L

D

N

D

I

R

D

V

L

L

L

E

E

L

L

L

I

N

S

Q

K

R

R

A

A

R

N

C

L

A

V

Q

Q

R

E

V

I

A

G

D

K

I

I

K

K

V

L

A

R

A

-

G

-

E

-

T

-

D

-

C

-

F

-

Y

F

R

D

P

P

E

L

R

R

E

E

A

R

E

E

I

M

L

L

Q

N

R

T

L

I

T

L

A

A

H

A

N

N

P

E

G

G

P

P

L

F

G

E

R

D

E

S

A

T

V

V

V

Q

R

K

F

L

F

F

R

K

E

E

V

I

M

F

S

K

E

A

C

G

L

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

6al9B Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
37% identity, 22% coverage: 4:81/362 of query aligns to 2:75/91 of 6al9B

query
sites
6al9B

D

D

P

S

S

L

L

L

A

E

E

N

L

L

R

R

K

A

R

E

I

I

D

D

E

A

L

L

D

D

D

N

R

E

V

L

L

S

E

D

L

L

N

D

Q

K

R

R

A

L

R

E

C

I

A

A

Q

L

R

K

V
x
I

A

A

D

L

I

I

K

K

V

-

A

-

G

-

E

Q

T

E

D

S

C

P

F

I

Y

Y

R

C

P

P

E

K

R

R

E

E

A

Q

E

E

I

I

L

L

Q

K

R

R

L

L

T

S

A

Q

H

R

N

D

P

F

G

K

P

H

L

L

G

N

R

G

E

E

A

I

V

L

V

T

R

G

F

F

F

Y

R

T

E

E

V

V

binding pyruvic acid: I33 (≠ V35)

Sites not aligning to the query:

binding pyruvic acid: 83

6al9A Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
37% identity, 22% coverage: 4:81/362 of query aligns to 1:74/90 of 6al9A

query
sites
6al9A

D

D

P

S

S

L

L

L

A

E

E

N

L

L

R

R

K

A

R

E

I

I

D

D

E

A

L

L

D

D

D

N

R

E

V

L

L

S

E

D

L

L

N

D

Q

K

R
|
R

A

L

R

E

C

I

A

A

Q

L

R

K

V

I

A

A

D

L

I

I

K

K

V

-

A

-

G

-

E

Q

T

E

D

S

C

P

F

I

Y

Y

R
x
C

P

P

E

K

R
|
R

E
|
E

A

Q

E

E

I

I

L

L

Q

K

R

R

L

L

T

S

A

Q

H

R

N

D

P

F

G

K

P

H

L

L

G

N

R

G

E

E

A

I

V

L

V

T

R

G

F

F

F

Y

R

T

E

E

V

V

binding prephenic acid: R25 (= R28), C43 (≠ R50), R46 (= R53), E47 (= E54)

Sites not aligning to the query:

binding prephenic acid: 75, 78, 82

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
27% identity, 24% coverage: 3:90/362 of query aligns to 1:86/87 of 5gmuB

query
sites
5gmuB

N

S

D

N

P

T

S

E

L

L

A

E

E

L

L

L

R

R

K

Q

R

K

I

A

D

D

E

E

L

L

D

N

D

L

R

Q

V

I

L

L

E

K

L

L

L

I

N

N

Q

E

R
|
R

A

G

R

N

C

V

A

V

Q

K

R

E

V

I

A

G

D

K

I

A

K

K

V

E

A

A

A

Q

G

G

E

V

T

N

D
x
R

C

-

F

-

Y
x
F

R
x
D

P

P

E

V

R

R

E
|
E

A

R

E

T

I

M

L

L

Q

N

R

N

L

I

T

I

A

E

H

N

N

N

P

D

G

G

P

P

L

F

G

E

R

N

E

S

A

T

V

I

V

Q

R

H

F

I

F

F

R

K

E

E

V

I

M
x
F

S
x
K

E

A

C

G

L
|
L

A

E

L

L

E
x
Q

K

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R28), R44 (≠ D46), F45 (≠ Y49), D46 (≠ R50), E50 (= E54), F78 (≠ M82), K79 (≠ S83), L82 (= L86), Q85 (≠ E89)

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
27% identity, 24% coverage: 3:90/362 of query aligns to 2:87/358 of P39912

query
sites
P39912

N
x
S

D

N

P

T

S

E

L

L

A

E

E

L

L

L

R

R

K

Q

R

K

I

A

D

D

E

E

L

L

D

N

D

L

R

Q

V

I

L

L

E

K

L

L

L

I

N

N

Q

E

R

R

A

G

R

N

C

V

A

V

Q

K

R

E

V

I

A

G

D

K

I

A

K

K

V

E

A

A

A

Q

G

G

E

V

T

N

D

R

C

-

F

-

Y

F

R

D

P

P

E

V

R

R

E

E

A

R

E

T

I

M

L

L

Q

N

R

N

L

I

T

I

A

E

H

N

N

N

P

D

G

G

P

P

L

F

G

E

R

N

E

S

A

T

V

I

V

Q

R

H

F

I

F

F

R

K

E

E

V

I

M

F

S

K

E

A

C

G

L

L

A

E

L

L

E

Q

K

E

S2 (≠ N3) modified: Phosphoserine

Query Sequence

>WP_010960694.1 NCBI__GCF_000008325.1:WP_010960694.1
MANDPSLAELRKRIDELDDRVLELLNQRARCAQRVADIKVAAGETDCFYRPEREAEILQR
LTAHNPGPLGREAVVRFFREVMSECLALEKPLSVAFLGPEGTFTQQAAYRHFGHAIQAVP
MPAIDEIFRAVESGACHYGVVPVENSTEGVITHTLDSFVRFSLIIAGEVQLRIHHNLLCR
TPTALTELTEVFSHPQSLAQCRGWLDRFLPGVRRTPLGSNAEAARRAAETAGTAAIAGEV
AAGLYGLEILNRNIEDEPDNTTRFLVIGGQPVGPTGHDKTSLLLSTRNDPGALFRLIEPF
ARLGISMTKIESRPSRRGMWDYFFFIDVEGHQADPTLAQALAEVREHCCMMRILGSYPRA
LS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory