SitesBLAST
Comparing WP_010961089.1 NCBI__GCF_000008325.1:WP_010961089.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
47% identity, 97% coverage: 10:543/553 of query aligns to 4:540/555 of 4rjiC
- binding magnesium ion: D438 (= D441), D465 (= D468), T467 (≠ G470)
- binding thiamine diphosphate: P24 (= P30), E48 (= E54), P74 (= P80), S387 (≠ A390), H388 (= H391), Q411 (= Q414), G437 (= G440), D438 (= D441), G439 (= G442), G440 (≠ S443), T467 (≠ G470), Y468 (= Y471), D469 (≠ N472), M470 (≠ L473), V471 (≠ I474), Y534 (= Y537)
4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
47% identity, 97% coverage: 10:543/553 of query aligns to 3:539/552 of 4rjkF
- binding magnesium ion: D437 (= D441), D464 (= D468), T466 (≠ G470)
- binding pyruvic acid: A25 (≠ G32), K26 (≠ A33)
- binding thiamine diphosphate: P23 (= P30), E47 (= E54), P73 (= P80), G385 (= G389), S386 (≠ A390), H387 (= H391), Q410 (= Q414), L412 (≠ M416), G436 (= G440), D437 (= D441), G438 (= G442), G439 (≠ S443), T466 (≠ G470), Y467 (= Y471), D468 (≠ N472), M469 (≠ L473), V470 (≠ I474), Y533 (= Y537)
4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
47% identity, 97% coverage: 10:543/553 of query aligns to 3:539/553 of 4rjkG
- binding magnesium ion: D437 (= D441), D464 (= D468), T466 (≠ G470)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E54), Q110 (≠ M117)
- binding thiamine diphosphate: I384 (≠ V388), G385 (= G389), S386 (≠ A390), H387 (= H391), Q410 (= Q414), L412 (≠ M416), G436 (= G440), D437 (= D441), G438 (= G442), G439 (≠ S443), T466 (≠ G470), Y467 (= Y471), D468 (≠ N472), M469 (≠ L473), V470 (≠ I474), Y533 (= Y537)
5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
46% identity, 96% coverage: 10:540/553 of query aligns to 7:538/548 of 5d6rB
- active site: I26 (≠ V29), G28 (= G31), A29 (≠ G32), K30 (≠ A33), I31 (= I34), E51 (= E54), T74 (= T77), H113 (= H116), Q114 (≠ M117), S115 (≠ N118), Q163 (≠ N166), L254 (≠ Y259), E281 (= E286), M386 (≠ V388), Q412 (= Q414), M414 (= M416), D439 (= D441), D466 (= D468), G468 (= G470), Y469 (= Y471), M471 (≠ L473), V472 (≠ I474), Q475 (≠ L477), Y535 (= Y537)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ V388), G387 (= G389), S388 (≠ A390), Q412 (= Q414), M414 (= M416), D439 (= D441), G440 (= G442), G468 (= G470), Y469 (= Y471), N470 (= N472), M471 (≠ L473), Y535 (= Y537)
- binding magnesium ion: R63 (= R66), Q212 (≠ T217), D439 (= D441), D466 (= D468), G468 (= G470)
1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
45% identity, 96% coverage: 10:540/553 of query aligns to 8:541/549 of 1ozgA
- active site: I27 (≠ V29), G29 (= G31), A30 (≠ G32), K31 (≠ A33), I32 (= I34), E52 (= E54), T75 (= T77), H114 (= H116), Q115 (≠ M117), S116 (≠ N118), Q164 (≠ N166), L257 (≠ Y259), E284 (= E286), M389 (≠ V388), Q415 (= Q414), M417 (= M416), D442 (= D441), D469 (= D468), G471 (= G470), Y472 (= Y471), M474 (≠ L473), V475 (≠ I474), Q478 (≠ L477), Y538 (= Y537)
- binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (≠ V388), G390 (= G389), S391 (≠ A390), F392 (≠ H391), Q415 (= Q414), M417 (= M416), G441 (= G440), D442 (= D441), G443 (= G442), D469 (= D468), G471 (= G470), Y472 (= Y471), N473 (= N472), M474 (≠ L473), V475 (≠ I474), Y538 (= Y537)
- binding magnesium ion: D442 (= D441), D469 (= D468), G471 (= G470)
- binding phosphate ion: G253 (= G255), R254 (= R256), Q261 (= Q263), R347 (vs. gap), R398 (= R397), Y401 (≠ F400)
1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
45% identity, 96% coverage: 10:540/553 of query aligns to 7:537/545 of 1ozfA
- active site: I26 (≠ V29), G28 (= G31), A29 (≠ G32), K30 (≠ A33), I31 (= I34), E51 (= E54), T74 (= T77), H113 (= H116), Q114 (≠ M117), S115 (≠ N118), Q163 (≠ N166), L253 (≠ Y259), E280 (= E286), M385 (≠ V388), Q411 (= Q414), M413 (= M416), D438 (= D441), D465 (= D468), G467 (= G470), Y468 (= Y471), M470 (≠ L473), V471 (≠ I474), Q474 (≠ L477), Y534 (= Y537)
- binding magnesium ion: D438 (= D441), D465 (= D468), G467 (= G470)
- binding phosphate ion: G249 (= G255), R250 (= R256), Q257 (= Q263), R343 (vs. gap), R394 (= R397), L396 (≠ F399), Y397 (≠ F400)
- binding thiamine diphosphate: G386 (= G389), S387 (≠ A390), F388 (≠ H391), Q411 (= Q414), M413 (= M416), G437 (= G440), D438 (= D441), G439 (= G442), D465 (= D468), G467 (= G470), Y468 (= Y471), N469 (= N472), M470 (≠ L473), V471 (≠ I474), Y534 (= Y537)
5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
44% identity, 98% coverage: 1:540/553 of query aligns to 7:544/557 of 5dx6B
- active site: I38 (≠ V29), G40 (= G31), A41 (≠ G32), K42 (≠ A33), I43 (= I34), E63 (= E54), T86 (= T77), H125 (= H116), Q126 (≠ M117), S127 (≠ N118), Q175 (≠ N166), L268 (≠ Y259), E295 (= E286), M392 (≠ V388), Q418 (= Q414), M420 (= M416), D445 (= D441), D472 (= D468), G474 (= G470), Y475 (= Y471), M477 (≠ L473), V478 (≠ I474), Q481 (≠ L477), Y541 (= Y537)
- binding 3-fluoro-2-oxopropanoic acid: G264 (= G255), R265 (= R256), Q272 (= Q263), A400 (= A396), R401 (= R397), Y404 (≠ F400)
- binding magnesium ion: S135 (≠ A126), T138 (= T129), D445 (= D441), D472 (= D468), G474 (= G470)
- binding thiamine diphosphate: G393 (= G389), S394 (≠ A390), F395 (≠ H391), Q418 (= Q414), M420 (= M416), G444 (= G440), D445 (= D441), G446 (= G442), D472 (= D468), G474 (= G470), Y475 (= Y471), N476 (= N472), M477 (≠ L473), V478 (≠ I474), Y541 (= Y537)
5wdgA Acetolactate synthase from klebsiella pneumoniae in complex with a reaction intermediate
45% identity, 96% coverage: 10:540/553 of query aligns to 8:530/538 of 5wdgA
- active site: I27 (≠ V29), G29 (= G31), A30 (≠ G32), K31 (≠ A33), I32 (= I34), E52 (= E54), T75 (= T77), Q157 (≠ N166), L246 (≠ Y259), E273 (= E286), M378 (≠ V388), Q404 (= Q414), M406 (= M416), D431 (= D441), D458 (= D468), G460 (= G470), Y461 (= Y471), M463 (≠ L473), V464 (≠ I474), Q467 (≠ L477), Y527 (= Y537)
- binding (2S,3S)-2,3-dihydroxy-3-[(7S,8R,9aS)-8-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-2,7-dimethyl-5,7,8,10-tetrahydro-9aH-pyrimido[4,5-d][1,3]thiazolo[3,2-a]pyrimidin-9a-yl]-2-methylbutanoic acid: M378 (≠ V388), S380 (≠ A390), F381 (≠ H391), Q404 (= Q414), M406 (= M416), G430 (= G440), D431 (= D441), G432 (= G442), G433 (≠ S443), D458 (= D468), G460 (= G470), Y461 (= Y471), N462 (= N472), M463 (≠ L473), V464 (≠ I474), Y527 (= Y537)
- binding magnesium ion: R64 (= R66), S117 (≠ A126), T120 (= T129), Q204 (≠ T217), D431 (= D441), D458 (= D468), G460 (= G470)
- binding pyruvic acid: G94 (≠ R96), R147 (≠ C156)
5dx6A Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
45% identity, 96% coverage: 10:540/553 of query aligns to 8:533/541 of 5dx6A
- active site: I27 (≠ V29), G29 (= G31), A30 (≠ G32), K31 (≠ A33), I32 (= I34), E52 (= E54), T75 (= T77), Q159 (≠ N166), L249 (≠ Y259), E276 (= E286), M381 (≠ V388), Q407 (= Q414), M409 (= M416), D434 (= D441), D461 (= D468), G463 (= G470), Y464 (= Y471), M466 (≠ L473), V467 (≠ I474), Q470 (≠ L477), Y530 (= Y537)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1R)-2-fluoro-1-hydroxyethyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M381 (≠ V388), G382 (= G389), S383 (≠ A390), F384 (≠ H391), Q407 (= Q414), M409 (= M416), G433 (= G440), D434 (= D441), G435 (= G442), D461 (= D468), G463 (= G470), Y464 (= Y471), N465 (= N472), Y530 (= Y537)
- binding magnesium ion: S119 (≠ A126), T122 (= T129), D434 (= D441), D461 (= D468), G463 (= G470)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
32% identity, 97% coverage: 9:542/553 of query aligns to 7:526/539 of 6lpiB
- active site: I27 (≠ V29), G29 (= G31), G30 (= G32), S31 (≠ A33), I32 (= I34), E53 (= E54), C76 (≠ T77), F115 (≠ H116), Q116 (≠ M117), E117 (≠ N118), K165 (≠ N166), M256 (≠ Y259), A283 (≠ E284), V375 (= V388), G401 (≠ Q414), M403 (= M416), D428 (= D441), N455 (≠ D468), A457 (≠ G470), L458 (≠ Y471), L460 (= L473), V461 (≠ I474), Q464 (≠ L477)
- binding flavin-adenine dinucleotide: R155 (≠ C156), G212 (= G212), G213 (≠ A215), G214 (≠ S216), T236 (= T238), L237 (≠ F239), M238 (≠ E240), L254 (≠ V257), M256 (≠ Y259), H257 (≠ V260), G276 (= G279), A277 (vs. gap), R278 (vs. gap), D280 (= D281), R282 (≠ I283), A283 (≠ E284), D300 (= D301), I301 (≠ A302), D319 (= D320), V320 (≠ I321), M380 (≠ I393), G398 (≠ M411)
- binding magnesium ion: D428 (= D441), N455 (≠ D468)
- binding thiamine diphosphate: E53 (= E54), C76 (≠ T77), P79 (= P80), G376 (= G389), Q377 (≠ A390), H378 (= H391), G401 (≠ Q414), M403 (= M416), G427 (= G440), D428 (= D441), G429 (= G442), S430 (= S443), M433 (= M446), N455 (≠ D468), A457 (≠ G470), L458 (≠ Y471), G459 (≠ N472), L460 (= L473), V461 (≠ I474)
7egvA Acetolactate synthase from trichoderma harzianum with inhibitor harzianic acid (see paper)
29% identity, 97% coverage: 3:536/553 of query aligns to 2:549/590 of 7egvA
- active site: Y28 (≠ V29), G30 (= G31), G31 (= G32), A32 (= A33), I33 (= I34), E54 (= E54), T77 (= T77), F116 (≠ H116), Q117 (≠ M117), K166 (≠ N166), E220 (≠ A222), M256 (≠ Y259), V283 (≠ I283), V400 (= V388), L425 (≠ H413), G426 (≠ Q414), M428 (= M416), Q483 (≠ Y471), M485 (≠ L473), V486 (≠ I474), W489 (≠ L477), L511 (= L498), G516 (= G503), I517 (≠ A504)
- binding flavin-adenine dinucleotide: R156 (≠ C156), G209 (= G212), Q210 (≠ L213), G211 (≠ R214), T236 (= T238), L237 (≠ F239), H238 (≠ E240), G276 (= G279), S277 (≠ Y280), R278 (vs. gap), D280 (vs. gap), R282 (≠ P282), V283 (≠ I283), E309 (≠ D301), I310 (≠ A302), D328 (= D320), V329 (≠ I321), M405 (≠ I393), G423 (≠ M411), G424 (= G412)
- binding (2S)-3-methyl-2-[[(2S,4R)-1-methyl-4-[(2E,4E)-octa-2,4-dienoyl]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]methyl]-2-oxidanyl-butanoic acid: F493 (≠ D481), Y494 (= Y482)
- binding magnesium ion: D453 (= D441), N480 (≠ D468), E482 (≠ G470)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: P29 (= P30), E54 (= E54), Q117 (≠ M117), V400 (= V388), G401 (= G389), Q402 (≠ A390), H403 (= H391), G426 (≠ Q414), M428 (= M416), D453 (= D441), A454 (≠ G442), S455 (= S443), E482 (≠ G470), Q483 (≠ Y471), G484 (≠ N472), M485 (≠ L473), V486 (≠ I474)
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
30% identity, 95% coverage: 9:536/553 of query aligns to 13:557/598 of 6desA
- active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (= A33), I38 (= I34), E59 (= E54), T82 (= T77), F121 (≠ H116), Q122 (≠ M117), E123 (≠ N118), K171 (≠ N166), K229 (≠ A222), M265 (≠ Y259), V292 (vs. gap), V408 (= V388), L433 (≠ H413), G434 (≠ Q414), M436 (= M416), D461 (= D441), N488 (≠ D468), E490 (≠ G470), Q491 (≠ Y471), M493 (≠ L473), V494 (≠ I474), W497 (≠ L477), L519 (= L498), N524 (≠ G503), V525 (≠ A504)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (≠ Y259), D290 (≠ P282), R291 (vs. gap), W497 (≠ L477)
- binding flavin-adenine dinucleotide: R161 (≠ C156), G218 (= G212), A219 (≠ L213), G220 (≠ R214), N223 (≠ S216), T245 (= T238), L246 (≠ F239), Q247 (≠ E240), L263 (≠ V257), G285 (= G279), A286 (vs. gap), R287 (vs. gap), D289 (= D281), R291 (vs. gap), V292 (vs. gap), E318 (≠ D301), I319 (≠ A302), N323 (≠ A306), D337 (= D320), V338 (≠ I321), Q412 (≠ E392), M413 (≠ I393), G431 (≠ M411)
- binding magnesium ion: D461 (= D441), N488 (≠ D468), E490 (≠ G470)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (= V388), G409 (= G389), Q410 (≠ A390), H411 (= H391), G434 (≠ Q414), M436 (= M416), G460 (= G440), D461 (= D441), A462 (≠ G442), S463 (= S443), N488 (≠ D468), E490 (≠ G470), Q491 (≠ Y471), G492 (≠ N472), M493 (≠ L473), V494 (≠ I474)
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
30% identity, 95% coverage: 9:536/553 of query aligns to 15:559/600 of 6derA
- active site: Y35 (≠ V29), G37 (= G31), G38 (= G32), A39 (= A33), I40 (= I34), E61 (= E54), T84 (= T77), F123 (≠ H116), Q124 (≠ M117), E125 (≠ N118), K173 (≠ N166), K231 (≠ A222), M267 (≠ Y259), V294 (vs. gap), V410 (= V388), L435 (≠ H413), G436 (≠ Q414), M438 (= M416), D463 (= D441), N490 (≠ D468), E492 (≠ G470), Q493 (≠ Y471), M495 (≠ L473), V496 (≠ I474), W499 (≠ L477), L521 (= L498), N526 (≠ G503), V527 (≠ A504)
- binding flavin-adenine dinucleotide: R163 (≠ C156), G220 (= G212), A221 (≠ L213), G222 (≠ R214), N225 (≠ S216), T247 (= T238), L248 (≠ F239), Q249 (≠ E240), L265 (≠ V257), H268 (≠ V260), G287 (= G279), A288 (vs. gap), R289 (vs. gap), D291 (= D281), R293 (vs. gap), V294 (vs. gap), E320 (≠ D301), I321 (≠ A302), N325 (≠ A306), G338 (= G319), D339 (= D320), V340 (≠ I321), Q414 (≠ E392), M415 (≠ I393), G433 (≠ M411)
- binding Metosulam: R293 (vs. gap), M495 (≠ L473), W499 (≠ L477)
- binding magnesium ion: D463 (= D441), N490 (≠ D468), E492 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (= V388), G411 (= G389), Q412 (≠ A390), H413 (= H391), G436 (≠ Q414), M438 (= M416), G462 (= G440), D463 (= D441), A464 (≠ G442), S465 (= S443), N490 (≠ D468), E492 (≠ G470), Q493 (≠ Y471), G494 (≠ N472), M495 (≠ L473), V496 (≠ I474)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (= V388), G411 (= G389), Q412 (≠ A390), H413 (= H391), G436 (≠ Q414), M438 (= M416), G462 (= G440), D463 (= D441), A464 (≠ G442), S465 (= S443), M468 (= M446), N490 (≠ D468), E492 (≠ G470), Q493 (≠ Y471), G494 (≠ N472), V496 (≠ I474)
Sites not aligning to the query:
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
30% identity, 95% coverage: 9:536/553 of query aligns to 13:557/598 of 6depA
- active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (= A33), I38 (= I34), E59 (= E54), T82 (= T77), F121 (≠ H116), Q122 (≠ M117), E123 (≠ N118), K171 (≠ N166), K229 (≠ A222), M265 (≠ Y259), V292 (vs. gap), V408 (= V388), L433 (≠ H413), G434 (≠ Q414), M436 (= M416), D461 (= D441), N488 (≠ D468), E490 (≠ G470), Q491 (≠ Y471), M493 (≠ L473), V494 (≠ I474), W497 (≠ L477), L519 (= L498), N524 (≠ G503), V525 (≠ A504)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (≠ P282), R291 (vs. gap), M493 (≠ L473), W497 (≠ L477)
- binding flavin-adenine dinucleotide: R161 (≠ C156), G218 (= G212), A219 (≠ L213), G220 (≠ R214), N223 (≠ S216), T245 (= T238), L246 (≠ F239), Q247 (≠ E240), L263 (≠ V257), G264 (= G258), G285 (= G279), A286 (vs. gap), R287 (vs. gap), D289 (= D281), R291 (vs. gap), V292 (vs. gap), E318 (≠ D301), I319 (≠ A302), N323 (≠ A306), D337 (= D320), V338 (≠ I321), M413 (≠ I393), G431 (≠ M411)
- binding magnesium ion: D461 (= D441), N488 (≠ D468), E490 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (= V388), G409 (= G389), Q410 (≠ A390), H411 (= H391), G434 (≠ Q414), M436 (= M416), G460 (= G440), D461 (= D441), A462 (≠ G442), S463 (= S443), M466 (= M446), N488 (≠ D468), E490 (≠ G470), Q491 (≠ Y471), G492 (≠ N472), M493 (≠ L473), V494 (≠ I474)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (= V388), G409 (= G389), Q410 (≠ A390), H411 (= H391), G434 (≠ Q414), M436 (= M416), G460 (= G440), D461 (= D441), A462 (≠ G442), S463 (= S443), M466 (= M446), N488 (≠ D468), E490 (≠ G470), Q491 (≠ Y471), G492 (≠ N472), M493 (≠ L473), V494 (≠ I474)
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
29% identity, 95% coverage: 9:536/553 of query aligns to 11:552/593 of 6deoA
- active site: Y31 (≠ V29), G33 (= G31), G34 (= G32), A35 (= A33), I36 (= I34), E57 (= E54), T80 (= T77), F119 (≠ H116), Q120 (≠ M117), E121 (≠ N118), K169 (≠ N166), K224 (≠ A222), M260 (≠ Y259), V287 (vs. gap), V403 (= V388), L428 (≠ H413), G429 (≠ Q414), M431 (= M416), D456 (= D441), N483 (≠ D468), E485 (≠ G470), Q486 (≠ Y471), M488 (≠ L473), V489 (≠ I474), W492 (≠ L477), L514 (= L498), N519 (≠ G503), V520 (≠ A504)
- binding flavin-adenine dinucleotide: R159 (≠ C156), G213 (= G212), A214 (≠ L213), G215 (≠ R214), N218 (≠ S216), T240 (= T238), L241 (≠ F239), Q242 (≠ E240), L258 (≠ V257), G280 (= G279), A281 (vs. gap), R282 (vs. gap), D284 (= D281), R286 (vs. gap), V287 (vs. gap), E313 (≠ D301), I314 (≠ A302), N318 (≠ A306), D332 (= D320), V333 (≠ I321), M408 (≠ I393), G426 (≠ M411)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (≠ Y259), D285 (≠ P282), R286 (vs. gap), M488 (≠ L473), W492 (≠ L477)
- binding magnesium ion: D456 (= D441), N483 (≠ D468), E485 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (= V388), G404 (= G389), Q405 (≠ A390), H406 (= H391), G429 (≠ Q414), M431 (= M416), G455 (= G440), D456 (= D441), A457 (≠ G442), S458 (= S443), M461 (= M446), N483 (≠ D468), E485 (≠ G470), Q486 (≠ Y471), G487 (≠ N472), M488 (≠ L473), V489 (≠ I474)
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
30% identity, 95% coverage: 9:536/553 of query aligns to 15:558/599 of 6denA
- active site: Y35 (≠ V29), G37 (= G31), G38 (= G32), A39 (= A33), I40 (= I34), E61 (= E54), T84 (= T77), F123 (≠ H116), Q124 (≠ M117), E125 (≠ N118), K173 (≠ N166), K230 (≠ A222), M266 (≠ Y259), V293 (vs. gap), V409 (= V388), L434 (≠ H413), G435 (≠ Q414), M437 (= M416), D462 (= D441), N489 (≠ D468), E491 (≠ G470), Q492 (≠ Y471), M494 (≠ L473), V495 (≠ I474), W498 (≠ L477), L520 (= L498), N525 (≠ G503), V526 (≠ A504)
- binding flavin-adenine dinucleotide: R163 (≠ C156), G219 (= G212), A220 (≠ L213), G221 (≠ R214), N224 (≠ S216), T246 (= T238), L247 (≠ F239), Q248 (≠ E240), L264 (≠ V257), G286 (= G279), A287 (vs. gap), R288 (vs. gap), D290 (= D281), R292 (vs. gap), V293 (vs. gap), E319 (≠ D301), I320 (≠ A302), N324 (≠ A306), D338 (= D320), V339 (≠ I321), M414 (≠ I393), G432 (≠ M411)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (≠ Y259), D291 (≠ P282), R292 (vs. gap), W498 (≠ L477)
- binding magnesium ion: D462 (= D441), N489 (≠ D468), E491 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (= V388), G410 (= G389), Q411 (≠ A390), H412 (= H391), G435 (≠ Q414), M437 (= M416), G461 (= G440), D462 (= D441), A463 (≠ G442), S464 (= S443), N489 (≠ D468), E491 (≠ G470), Q492 (≠ Y471), G493 (≠ N472), M494 (≠ L473), V495 (≠ I474)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
29% identity, 95% coverage: 9:536/553 of query aligns to 13:556/597 of 6demA
- active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (= A33), I38 (= I34), E59 (= E54), T82 (= T77), F121 (≠ H116), Q122 (≠ M117), E123 (≠ N118), K171 (≠ N166), K228 (≠ A222), M264 (≠ Y259), V291 (vs. gap), V407 (= V388), L432 (≠ H413), G433 (≠ Q414), M435 (= M416), D460 (= D441), N487 (≠ D468), E489 (≠ G470), Q490 (≠ Y471), M492 (≠ L473), V493 (≠ I474), W496 (≠ L477), L518 (= L498), N523 (≠ G503), V524 (≠ A504)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ Y259), D289 (≠ P282), R290 (vs. gap), M492 (≠ L473), W496 (≠ L477)
- binding flavin-adenine dinucleotide: R161 (≠ C156), G217 (= G212), A218 (≠ L213), G219 (≠ R214), N222 (≠ S216), T244 (= T238), L245 (≠ F239), Q246 (≠ E240), L262 (≠ V257), G284 (= G279), A285 (vs. gap), R286 (vs. gap), D288 (= D281), R290 (vs. gap), V291 (vs. gap), E317 (≠ D301), I318 (≠ A302), N322 (≠ A306), D336 (= D320), V337 (≠ I321), M412 (≠ I393), G430 (≠ M411)
- binding magnesium ion: D460 (= D441), N487 (≠ D468), E489 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V388), G408 (= G389), Q409 (≠ A390), H410 (= H391), M435 (= M416), G459 (= G440), D460 (= D441), A461 (≠ G442), S462 (= S443), M465 (= M446), N487 (≠ D468), E489 (≠ G470), Q490 (≠ Y471), G491 (≠ N472), M492 (≠ L473), V493 (≠ I474)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
29% identity, 95% coverage: 9:536/553 of query aligns to 13:556/597 of 6delA
- active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (= A33), I38 (= I34), E59 (= E54), T82 (= T77), F121 (≠ H116), Q122 (≠ M117), E123 (≠ N118), K171 (≠ N166), K228 (≠ A222), M264 (≠ Y259), V291 (vs. gap), V407 (= V388), L432 (≠ H413), G433 (≠ Q414), M435 (= M416), D460 (= D441), N487 (≠ D468), E489 (≠ G470), Q490 (≠ Y471), M492 (≠ L473), V493 (≠ I474), W496 (≠ L477), L518 (= L498), N523 (≠ G503), V524 (≠ A504)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ P282), R290 (vs. gap), W496 (≠ L477)
- binding flavin-adenine dinucleotide: R161 (≠ C156), G217 (= G212), A218 (≠ L213), G219 (≠ R214), N222 (≠ S216), T244 (= T238), L245 (≠ F239), Q246 (≠ E240), L262 (≠ V257), G284 (= G279), A285 (vs. gap), R286 (vs. gap), D288 (= D281), R290 (vs. gap), V291 (vs. gap), E317 (≠ D301), I318 (≠ A302), N322 (≠ A306), D336 (= D320), V337 (≠ I321), M412 (≠ I393), G430 (≠ M411)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (= V388), G408 (= G389), Q409 (≠ A390), H410 (= H391), G433 (≠ Q414), M435 (= M416), G459 (= G440), D460 (= D441), A461 (≠ G442), S462 (= S443), M465 (= M446), N487 (≠ D468), E489 (≠ G470), Q490 (≠ Y471), G491 (≠ N472), M492 (≠ L473), V493 (≠ I474)
- binding magnesium ion: D460 (= D441), N487 (≠ D468), E489 (≠ G470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V388), G408 (= G389), Q409 (≠ A390), H410 (= H391), G433 (≠ Q414), M435 (= M416), G459 (= G440), D460 (= D441), A461 (≠ G442), S462 (= S443), M465 (= M446), N487 (≠ D468), E489 (≠ G470), Q490 (≠ Y471), G491 (≠ N472), M492 (≠ L473), V493 (≠ I474)
6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 95% coverage: 9:536/553 of query aligns to 15:560/601 of 6deqA
- active site: Y35 (≠ V29), G37 (= G31), G38 (= G32), A39 (= A33), I40 (= I34), E61 (= E54), T84 (= T77), F123 (≠ H116), Q124 (≠ M117), E125 (≠ N118), K173 (≠ N166), K232 (≠ A222), M268 (≠ Y259), V295 (vs. gap), V411 (= V388), L436 (≠ H413), G437 (≠ Q414), M439 (= M416), D464 (= D441), N491 (≠ D468), E493 (≠ G470), Q494 (≠ Y471), M496 (≠ L473), V497 (≠ I474), W500 (≠ L477), L522 (= L498), N527 (≠ G503), V528 (≠ A504)
- binding flavin-adenine dinucleotide: R163 (≠ C156), G221 (= G212), A222 (≠ L213), G223 (≠ R214), N226 (≠ S216), T248 (= T238), L249 (≠ F239), Q250 (≠ E240), L266 (≠ V257), G288 (= G279), A289 (vs. gap), R290 (vs. gap), D292 (= D281), R294 (vs. gap), V295 (vs. gap), E321 (≠ D301), I322 (≠ A302), D340 (= D320), V341 (≠ I321), M416 (≠ I393), G434 (≠ M411)
- binding magnesium ion: D464 (= D441), N491 (≠ D468), E493 (≠ G470)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (≠ Y259), R294 (vs. gap), M496 (≠ L473), V497 (≠ I474), W500 (≠ L477)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (= V388), G412 (= G389), Q413 (≠ A390), H414 (= H391), M439 (= M416), G463 (= G440), D464 (= D441), A465 (≠ G442), S466 (= S443), N491 (≠ D468), E493 (≠ G470), Q494 (≠ Y471), G495 (≠ N472), M496 (≠ L473), V497 (≠ I474)
Sites not aligning to the query:
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
28% identity, 95% coverage: 9:536/553 of query aligns to 11:558/599 of 1n0hA
- active site: Y31 (≠ V29), G33 (= G31), G34 (= G32), A35 (= A33), I36 (= I34), E57 (= E54), T80 (= T77), F119 (≠ H116), Q120 (≠ M117), E121 (≠ N118), K169 (≠ N166), R230 (≠ A222), M266 (≠ Y259), V293 (≠ I283), V409 (= V388), L434 (≠ H413), G435 (≠ Q414), M437 (= M416), D462 (= D441), N489 (≠ D468), E491 (≠ G470), Q492 (≠ Y471), M494 (≠ L473), V495 (≠ I474), W498 (≠ L477), L520 (= L498), G525 (= G503), L526 (≠ A504)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (= V388), G410 (= G389), Q411 (≠ A390), H412 (= H391), G435 (≠ Q414), M437 (= M416), G461 (= G440), D462 (= D441), A463 (≠ G442), S464 (= S443), M467 (= M446), N489 (≠ D468), E491 (≠ G470), Q492 (≠ Y471), G493 (≠ N472), V495 (≠ I474)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (= G32), A35 (= A33), V109 (= V106), P110 (= P107), F119 (≠ H116), K169 (≠ N166), M266 (≠ Y259), D291 (= D281), R292 (≠ P282), V495 (≠ I474), W498 (≠ L477)
- binding flavin-adenine dinucleotide: R159 (≠ C156), G219 (= G212), A220 (≠ L213), G221 (vs. gap), N224 (vs. gap), T246 (= T238), L247 (≠ F239), Q248 (≠ E240), L264 (≠ V257), G265 (= G258), M266 (≠ Y259), H267 (≠ V260), G286 (= G279), A287 (≠ Y280), R288 (vs. gap), D290 (vs. gap), R292 (≠ P282), V293 (≠ I283), E319 (≠ D301), V320 (≠ A302), N324 (≠ A306), G337 (= G319), D338 (= D320), A339 (≠ I321), M414 (≠ I393), G432 (≠ M411), G433 (= G412)
- binding magnesium ion: D462 (= D441), N489 (≠ D468), E491 (≠ G470)
- binding thiamine diphosphate: Y31 (≠ V29), E57 (= E54), P83 (= P80)
Sites not aligning to the query:
Query Sequence
>WP_010961089.1 NCBI__GCF_000008325.1:WP_010961089.1
MRETIPPRTGADLLVDSLQALGVEYVFGVPGGAILPILNVLADRGPRFIVCRDETGAAFM
AQSWGRITGRPGVVLTTSGPGLINAVCGVATATEDRDPLVVITGQVPRAVQFKQSHMNLD
SVGLFAPITKWSVEVEEPNTVSEILVNAFRTAQTPCAGAVHVSVPNDMLTAPVTAQALAP
AEPAVWGTAPAAVVERAASLLNDAKAPAILLGLRASTPGAAAAVRRFLERHPLPVAMTFE
AAGTLSRDLVDQFVGRVGYVLNQPGDEVLRQADLVLTIGYDPIEYEPSAWISPQSQAIHL
DALPAAVDRAYHPAAELVGDIAANLAALGSLLRIEDRAGRPAVAAARRRLLEEQARGAAL
TGMPIHPLRFIHDLRATLDDEATVTCDVGAHEIWMARYFFCYAPRHLLFSMGHQTMGVAL
PWAIGAALARPGKKVVSVSGDGSFLMTCMELETAVRLKLPIVHIVWKDGGYNLIHSLQMR
DYGRSFGAEFGPTDFVKLAEAFGAIGYRIESADGIVPVLNRALAADAPVLIEVPIDYSDN
VHLVEAIDASAQH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory