SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010963429.1 NCBI__GCF_000008765.1:WP_010963429.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
42% identity, 99% coverage: 3:406/409 of query aligns to 7:423/428 of 7kb1C

query
sites
7kb1C

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binding pyridoxal-5'-phosphate: Y57 (= Y51), R59 (= R53)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G81), Q88 (≠ M82), Y112 (= Y106), N160 (= N152), D185 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), N369 (= N352), I370 (= I353), R404 (= R387)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
42% identity, 99% coverage: 3:406/409 of query aligns to 7:423/428 of 7kb1A

query
sites
7kb1A

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binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y51), R59 (= R53), G87 (= G81), Q88 (≠ M82), Y112 (= Y106), N160 (= N152), D185 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), N369 (= N352), I370 (= I353), R404 (= R387)

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
39% identity, 100% coverage: 3:409/409 of query aligns to 9:425/425 of 8wkoA

query
sites
8wkoA

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D

N

I

A

E

D

D

D

I

L

L

I

E

A

D

D

I

L

D

D

R

Q

A

A

L

L

E

A

A

Q

I

V

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G81), S88 (≠ M82), Y112 (= Y106), E155 (= E148), D184 (= D177), T186 (= T179), S206 (= S199), A207 (≠ T200), T208 (≠ S201), F209 (≠ Y203), G212 (= G206), M217 (≠ I211), V369 (≠ I353), A370 (≠ G354)
binding proline: H213 (≠ T207), Q284 (≠ T269), S288 (≠ T273)

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
36% identity, 99% coverage: 1:406/409 of query aligns to 1:420/421 of 2ctzA

query
sites
2ctzA

M

M

K

R

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

Y

Y

N

E

L

P

E

E

N

P

T

T

G

T

-

L

A

S

T

R

N

Q

V

V

P

P

I

I

Y

Y

M

P

S

T

N

T

A

S

Y

Y

A

V

H

F

S

K

S

S

P

P

Q

E

E

H

L

A

E

A

N

N

V

L

F

F

K

A

G

L

K

K

F

E

P

F

G

G

Y

N

V

I

Y

Y

S

S

R
|
R

F

I

S

M

N

N

P

P

T

T

V

V

L

D

E

V

F

L

E

E

R

K

R

R

M

L

A

A

S

A

I

L

E

E

G

G

G

G

L

K

T

A

A

A

T

L

S

A

A

T

A

A

S
|
S

G
|
G

M
x
H

S

A

A

A

I
x
Q

Y

F

M

L

A

A

I

L

T

T

N

T

I

L

V

A

H

Q

P

A

G

G

D

D

E

N

I

I

I

V

A

S

S

T

T

P

G

N

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

T

N

L

Q

I

F

S

K

-

V

N

T

L

L

Q

K

N

R

I

L

G

G

V

I

K

E

V

V

I

R

F

F

L

T

-

S

E

R

D

E

I

E

S

R

K

P

K

E

T

E

L

F

L

L

N

A

N

L

I

T

N

D

E

E

N

K

T

T

K

R

L

A

V

W

Y

W

T

V

E

E

T

S

I

I

G

G

N

N

P

P

K

A

L

L

D

N

I

I

L

P

D

D

I

L

E

E

S

A

V

L

G

A

R

Q

V

A

C

A

K

R

E

E

K

K

G

G

I

V

I

A

F

L

M

I

V

V

D
|
D

S

N

T
|
T

I

F

S

G

T

M

-

G

P

G

Y

Y

L

L

I

L

N

R

P

P

S

L

K

A

Y

W

G

G

A

A

D

A

V

L

I

V

I

T

H

H

S
|
S

T

L

S
x
T

K
|
K

Y

W

I

V

N

G

G

G

T

H

S

G

N

A

A

V

I

I

G

A

G

G

M

A

I

I

V

V

D

D

C

G

G

G

S

N

E

F

K

P

Y

W

K

E

N

G

H

G

R

R

Y

Y

E

P

N

L

F

L

Q

T

-

E

-

P

-

Q

-

P

-

G

-

Y

-

H

-

G

-

L

K

R

Y

L

A

T

E

E

K

A

Y

F

D

G

K

E

L

L

A

A

F

F

T

I

A

V

K

K

L

A

K

R

S

V

T

D

S

G

G

L

L

R

D

D

I

Q

G

G

A

Q

S

A

M

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

S

V

L

L

T

L

G

G

I

M

E

E

T

T

L

L

S

S

L

L

R

R

M

A

K

E

K

R

H

H

C

V

E

E

N

N

A

T

M

L

K

H

I

L

A

A

S

H

Y

W

L

L

E

L

K

E

S

Q

D

P

K

Q

V

V

T

A

N

W

V

V

N

N

Y

Y

P

P

N

G

L

L

K

P

S

H

S

H

K

P

Y

H

Y

H

D

D

L

R

A

A

T

Q

K

K

Y

Y

Y

F

A

K

D

G

G

K

A

P

S

G

G

A

I

V

L

L

T

T

F

F

R

G

L

L

-

K

G

G

T

G

K

Y

E

E

N

A

A

A

Y

K

K

R

F

F

L

I

A

S

K

R

L

L

K

K

L

L

I

I

L

S

D

H

T

L

T

A

N

N

I

V

G

G

D

D

C

T

K

R

T

T

I

L

I

A

I

I

H

H

P

P

A

A

S

S

T

T

I

T

C

H

I

S

N

Q

N

L

T

S

E

P

E

E

E

E

R

Q

E

A

K

Q

M

A

A

G

V

V

Y

S

D

P

D

E

L

M

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

I

V

E

E

D

D

I

L

L

K

E

A

D

E

I

L

D

K

R

E

A

A

L

L

active site: R54 (= R53), Y107 (= Y106), D180 (= D177), K206 (= K202)
binding pyridoxal-5'-phosphate: S81 (= S80), G82 (= G81), H83 (≠ M82), Q86 (≠ I85), Y107 (= Y106), D180 (= D177), T182 (= T179), S203 (= S199), T205 (≠ S201), K206 (= K202)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
36% identity, 99% coverage: 1:406/409 of query aligns to 1:420/421 of Q5SK88

query
sites
Q5SK88

M

M

K

R

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

Y

Y

N

E

L

P

E

E

N

P

T

T

G

T

-

L

A

S

T

R

N

Q

V

V

P

P

I

I

Y

Y

M

P

S

T

N

T

A

S

Y

Y

A

V

H

F

S

K

S

S

P

P

Q

E

E

H

L

A

E

A

N

N

V

L

F

F

K

A

G

L

K

K

F

E

P

F

G

G

Y

N

V

I

Y

Y

S

S

R

R

F

I

S

M

N

N

P

P

T

T

V

V

L

D

E

V

F

L

E

E

R

K

R

R

M

L

A

A

S

A

I

L

E

E

G

G

G

G

L

K

T

A

A

A

T

L

S

A

A

T

A

A

S

S

G

G

M

H

S

A

A

A

I

Q

Y

F

M

L

A

A

I

L

T

T

N

T

I

L

V

A

H

Q

P

A

G

G

D

D

E

N

I

I

I

V

A

S

S

T

T

P

G

N

L

L

Y

Y

G

G

G

G

T

T

Y

F

T

N

L

Q

I

F

S

K

-

V

N

T

L

L

Q

K

N

R

I

L

G

G

V

I

K

E

V

V

I

R

F

F

L

T

-

S

E

R

D

E

I

E

S

R

K

P

K

E

T

E

L

F

L

L

N

A

N

L

I

T

N

D

E

E

N

K

T

T

K

R

L

A

V

W

Y

W

T

V

E

E

T

S

I

I

G

G

N

N

P

P

K

A

L

L

D

N

I

I

L

P

D

D

I

L

E

E

S

A

V

L

G

A

R

Q

V

A

C

A

K

R

E

E

K

K

G

G

I

V

I

A

F

L

M

I

V

V

D

D

S

N

T

T

I

F

S

G

T

M

-

G

P

G

Y

Y

L

L

I

L

N

R

P

P

S

L

K

A

Y

W

G

G

A

A

D

A

V

L

I

V

I

T

H

H

S

S

T

L

S

T

K
|
K

Y

W

I

V

N

G

G

G

T

H

S

G

N

A

A

V

I

I

G

A

G

G

M

A

I

I

V

V

D

D

C

G

G

G

S

N

E

F

K

P

Y

W

K

E

N

G

H

G

R

R

Y

Y

E

P

N

L

F

L

Q

T

-

E

-

P

-

Q

-

P

-

G

-

Y

-

H

-

G

-

L

K

R

Y

L

A

T

E

E

K

A

Y

F

D

G

K

E

L

L

A

A

F

F

T

I

A

V

K

K

L

A

K

R

S

V

T

D

S

G

G

L

L

R

D

D

I

Q

G

G

A

Q

S

A

M

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

S

V

L

L

T

L

G

G

I

M

E

E

T

T

L

L

S

S

L

L

R

R

M

A

K

E

K

R

H

H

C

V

E

E

N

N

A

T

M

L

K

H

I

L

A

A

S

H

Y

W

L

L

E

L

K

E

S

Q

D

P

K

Q

V

V

T

A

N

W

V

V

N

N

Y

Y

P

P

N

G

L

L

K

P

S

H

S

H

K

P

Y

H

Y

H

D

D

L

R

A

A

T

Q

K

K

Y

Y

Y

F

A

K

D

G

G

K

A

P

S

G

G

A

I

V

L

L

T

T

F

F

R

G

L

L

-

K

G

G

T

G

K

Y

E

E

N

A

A

A

Y

K

K

R

F

F

L

I

A

S

K

R

L

L

K

K

L

L

I

I

L

S

D

H

T

L

T

A

N

N

I

V

G

G

D

D

C

T

K

R

T

T

I

L

I

A

I

I

H

H

P

P

A

A

S

S

T

T

I

T

C

H

I

S

N

Q

N

L

T

S

E

P

E

E

E

E

R

Q

E

A

K

Q

M

A

A

G

V

V

Y

S

D

P

D

E

L

M

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

I

V

E

E

D

D

I

L

L

K

E

A

D

E

I

L

D

K

R

E

A

A

L

L

K206 (= K202) modified: N6-(pyridoxal phosphate)lysine

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
38% identity, 99% coverage: 3:407/409 of query aligns to 8:427/429 of O13326

query
sites
O13326

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

-

A

G

G

N

Q

Y

E

N

P

L

D

E

A

N

A

T

T

G

S

A

S

T

R

N

A

V

V

P

P

I

I

Y

Y

M

A

S

T

N

T

A

S

Y

Y

A

V

H

F

S

R

S

D

P

C

Q

D

E

H

L

G

E

G

N

R

V

L

F

F

K

G

G

L

K

Q

F

E

P

P

G

G

Y

Y

V

I

Y

Y

S

S

R

R

F

M

S

M

N

N

P

P

T

T

V

A

L

D

E

V

F

F

E

E

R

K

R

R

M

I

A

A

S

A

I

L

E

E

G

H

G

G

L

A

T

A

A

A

T

I

S

A

A

T

A

S

S

S

G

G

M

T

S

S

A

A

I

L

Y

F

M

M

A

A

I

L

T

T

N

T

I

L

V

A

H

K

P

A

G

G

D

D

E

N

I

I

I

V

A

S

S

T

T

S

G

Y

L

L

Y

Y

G

G

G

G

T

T

Y

Y

T

N

L

L

I

F

S

K

-

V

N

T

L

L

Q

P

N

R

I

L

G

G

V

I

K

T

V

T

I

K

F

F

L

V

E

N

D

G

I

D

S

D

K

P

K

N

T

D

L

L

L

A

N

A

N

Q

I

I

N

D

E

E

N

N

T

T

K

K

L

A

V

V

Y

Y

T

V

E

E

T

S

I

I

G

G

N

N

P

P

K

M

L

Y

D

N

I

V

L

P

D

D

I

F

E

E

S

R

V

I

G

A

R

E

V

V

C

A

K

H

E

A

K

A

G

G

I

V

I

P

F

L

M

M

V

V

D

D

S

N

T

T

I

F

-

G

S

G

T

G

P

G

Y

Y

L

L

I

V

N

R

P

P

S

I

K

D

Y

H

G

G

A

A

D

D

V

I

I

V

I

T

H

H

S

S

T

A

S

T

K

K

Y

W

I

I

N

G

G

G

T

H

S

G

N

T

A

T

I

I

G

G

G

G

M

V

I

I

V

V

D

D

C

S

G

G

S

K

E

F

K

D

Y

W

K

K

N

K

H

N

-

S

-

K

R

R

Y

F

E

P

N

E

F

F

Q

N

K

E

-

P

-

H

-

P

-

G

-

Y

-

H

-

G

-

M

-

V

Y

F

A

T

E

E

K

T

Y

F

D

G

K

N

L

L

A

A

F

Y

T

A

A

F

K

A

L

C

K

R

S

T

T

Q

S

T

G

L

L

R

D

D

I

V

G

G

A

G

S

N

M

A

S

N

P

P

F

F

N

G

A

V

F

F

L

L

S

L

L

L

T

Q

G

G

I

L

E

E

T

T

L

L

S

S

L

L

R

R

M

M

K

E

K

R

H

H

C

V

E

Q

N

N

A

A

M

F

K

A

I

L

A

A

S

K

Y

Y

L

L

E

E

K

K

S

H

D

P

K

K

V

V

T

N

N

W

V

V

N

S

Y

Y

P

P

N

G

L

L

K

E

S

S

S

H

K

V

Y

S

Y

H

D

K

L

L

A

A

T

K

K

K

Y

Y

Y

L

A

K

D

N

G

G

A

Y

S

G

G

A

I

V

L

L

T

S

F

F

R

G

L

A

-

K

G

G

T

G

K

P

E

D

N

Q

A

S

Y

R

K

K

F

V

L

V

A

N

K

A

L

L

K

K

L

L

I

A

L

S

D

Q

T

L

T

A

N

N

I

V

G

G

D

D

C

A

K

K

T

T

I

L

I

V

I

I

H

A

P

P

A

A

S

Y

T

T

I

T

C

H

I

L

N

Q

N

L

T

T

E

D

E

E

E

E

R

Q

E

I

K

S

M

A

A

G

V

V

Y

T

D

K

D

D

L

L

L

I

R

R

L

V

S

A

V

V

G
|
G

I

I

E

E

D

H

I

I

E

D

D

D

I

I

L

I

E

A

D

D

I

F

D

A

R

Q

A

A

L

L

E

E

G411 (= G391) mutation to D: Impairs homocysteine synthase activity.

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 7:392/395 of 5m3zA

query
sites
5m3zA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G
x
H

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E
|
E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T
|
T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

V

S
x
T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T
x
V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R58 (= R53), Y111 (= Y106), D183 (= D177), K208 (= K202)
binding norleucine: Y111 (= Y106), H113 (≠ G108), K208 (= K202), V336 (≠ T351), S337 (≠ N352)
binding pyridoxal-5'-phosphate: G86 (= G81), I87 (≠ M82), Y111 (= Y106), E154 (= E148), D183 (= D177), T185 (= T179), S205 (= S199), T207 (≠ S201), K208 (= K202)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G81), I87 (≠ M82), Y111 (= Y106), D183 (= D177), S205 (= S199), T207 (≠ S201), K208 (= K202), V336 (≠ T351), S337 (≠ N352), R372 (= R387)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 4omaA

query
sites
4omaA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E

E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

V

S
x
T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T
x
V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G81), I88 (≠ M82), Y112 (= Y106), D184 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), V337 (≠ T351), S338 (≠ N352), R373 (= R387)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 3jwbA

query
sites
3jwbA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E

E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T

V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding norleucine: Y112 (= Y106), K209 (= K202), S338 (≠ N352), R373 (= R387)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 3jwaA

query
sites
3jwaA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E

E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T
x
V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y106), K209 (= K202), V337 (≠ T351), S338 (≠ N352), R373 (= R387)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 3jw9A

query
sites
3jw9A

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E

E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T
x
V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding S-ethyl-L-cysteine: Y112 (= Y106), K209 (= K202), V337 (≠ T351), S338 (≠ N352)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 4hf8A

query
sites
4hf8A

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R
|
R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

R

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E
|
E

T

T

I

P

G

A

N
|
N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I

F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T

V

N
x
S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G81), I88 (≠ M82), Y112 (= Y106), E155 (= E148), N159 (= N152), D184 (= D177), S206 (= S199), K209 (= K202), S338 (≠ N352), R373 (= R387)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 8:393/396 of 6egrA

query
sites
6egrA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

A

G

L

K

E

F

E

P

S

G

G

Y

Y

V

I

Y

Y

S

T

R

R

F

L

S

G

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E
|
E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T
|
T

I
x
F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

V

S
x
T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T

V

N

S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

Y

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

binding pyridoxal-5'-phosphate: G87 (= G81), I88 (≠ M82), Y112 (= Y106), E155 (= E148), D184 (= D177), T186 (= T179), F187 (≠ I180), S206 (= S199), T208 (≠ S201), K209 (= K202)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
38% identity, 100% coverage: 1:409/409 of query aligns to 6:397/399 of 5dx5A

query
sites
5dx5A

M

M

K

G

F

F

S

A

T

T

K

K

L

A

I

I

H

H

G

G

N

G

Y

H

-

I

N

G

L

D

E

K

N

Q

T

F

G

G

A

S

T

L

N

A

V

T

P

P

I

I

Y

Y

M

Q

S

T

N

S

A

T

Y

F

A

I

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

G

N

R

V

R

F

F

K

A

G

G

K

E

F

E

P

S

G

G

Y

Y

V

I

Y
|
Y

S

S

R
|
R

F

L

S

G

N

N

P

P

T

T

V

S

L

T

E

E

F

V

E

E

R

N

R

K

M

L

A

A

S

L

I

L

E

E

G

G

G

G

L

E

T

A

A

A

T

V

S

V

A

A

A

A

S
|
S

G
|
G

M
|
M

S

G

A

A

I

I

Y

A

M

A

A

S

I

L

T

W

N

S

I

A

V

L

H

K

P

S

G

G

D

D

E

H

I

V

I

V

A

A

S

S

T

D

G

T

L

L

Y
|
Y

G

G

G

C

T

T

Y

F

T

A

L

L

I

L

S

N

N

H

-

G

L

L

Q

T

N

R

I

Y

G

G

V

V

K

E

V

V

I

T

F

F

L

V

E

D

D

V

I

S

S

N

K

L

K

D

T

E

L

V

L

K

N

N

N

A

I

L

N

K

E

P

N

N

T

T

K

K

L

V

V

V

Y

Y

T

L

E

E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

K

I

V

L

T

D

D

I

I

E

R

S

K

V

I

G

S

R

N

V

M

C

V

K

H

E

E

-

S

-

N

K

K

G

E

I

C

I

F

F

V

M

F

V

V

D
|
D

S

N

T

T

I
x
F

S

C

T

T

P

P

Y

Y

L

I

I

Q

N

R

P

P

S

L

K

E

Y

L

G

G

A

A

D

D

V

V

I

V

I

V

H

H

S
|
S

T

A

S
x
T

K
|
K

Y

Y

I

L

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

A

G

G

M

F

I

A

V

V

D

-

C

-

G

G

S

K

E

E

K

E

Y

F

K

I

N

N

H

Q

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

-

D

-

K

-

L

-

A

-

F

-

T

-

A

V

K

K

L

L

K

F

S

G

T

I

S

K

G

D

L

M

D

-

I

T

G

G

A

S

S

V

M

T

S

G

P

P

F

F

N

E

A

S

F

F

L

L

S

I

L

I

T

R

G

G

I

M

E

K

T

T

L

L

S

Q

L

L

R

R

M

M

K

E

K

K

H

H

C

C

E

K

N

N

A

A

M

M

K

E

I

V

A

A

S

K

Y

F

L

L

E

E

K

S

S

H

D

P

K

A

V

V

T

E

N

K

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

E

S

S

S

F

K

K

Y

Y

Y

Y

D

Q

L

L

A

A

T

R

K

E

Y

Q

Y

M

A

K

-

L

D

P

G

G

A

A

S

-

G

-

I

M

L

I

T

S

F

F

R

E

L

L

-

K

G

G

T

G

K

V

E

E

N

E

A

G

Y

K

K

I

F

V

L

M

A

N

K

N

L

V

K

K

L

L

I

A

L

T

D

L

T

A

T

V

N

S

I

L

G

G

D

D

C

S

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

Y

T

T

E

A

E

E

E

E

R

R

E

K

K

A

M

A

A

G

V

I

Y

S

D

D

D

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

A

E

E

D

D

I

I

L

I

E

D

D

D

I

L

D

K

R

Q

A

A

L

L

E

D

A

L

I

I

active site: R59 (= R53), Y112 (= Y106), D186 (= D177), K211 (= K202)
binding pyridoxal-5'-phosphate: Y57 (= Y51), R59 (= R53), S86 (= S80), G87 (= G81), M88 (= M82), Y112 (= Y106), D186 (= D177), F189 (≠ I180), S208 (= S199), T210 (≠ S201), K211 (= K202)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
34% identity, 99% coverage: 3:407/409 of query aligns to 8:382/386 of 3mkjA

query
sites
3mkjA

F

F

S

N

T

T

K

Q

L

I

I

V

H

H

-

A

G

G

N

Q

Y

Q

N

P

L

D

E

P

N

S

T

T

G

G

A

A

T

L

N

S

V

T

P

P

I

I

Y

F

M

Q

S

T

N

S

A

T

Y

F

A

V

H

F

S

D

S

S

P

A

Q

E

E

Q

L

G

E

A

N

A

V

R

F

F

K

L

G

G

K

-

F

-

P

-

G

-

Y

-

V

-

Y

-

S

-

R

-

F

-

S

-

N

N

P

P

T

T

V

T

L

D

E

A

F

L

E

E

R

K

R

K

M

L

A

A

S

V

I

L

E

E

G

R

G

G

L

E

T

A

A

G

T

L

S

A

A

T

A

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

M

T

A

T

I

L

T

L

N

T

I

L

V

C

H

Q

P

Q

G

G

D

D

E

H

I

I

I

V

A

S

S

A

T

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

I

L

S

S

N

H

-

S

L

M

Q

P

N

K

I

F

G

G

V

I

K

N

V

V

I

S

F

F

L

V

E

D

D

A

I

A

S

K

K

P

K

E

T

E

L

I

L

R

N

A

N

A

I

M

N

R

E

P

N

E

T

T

K

K

L

V

V

V

Y

Y

T

I

E
|
E

T

T

I

P

G

A

N

N

P

P

K

T

L

L

D

S

I

L

L

V

D

D

I

I

E

E

S

T

V

V

G

A

R

G

V

I

C

A

K

H

E

Q

K

Q

G

G

I

A

I

L

F

L

M

V

V

V

D
|
D

S

N

T

T

I
x
F

S

M

T

S

P

P

Y

Y

L

C

I

Q

N

Q

P

P

S

L

K

Q

Y

L

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

V

S
x
T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

A

V

I

I

G

G

G

G

M

I

I

I

V

V

D

-

C

-

G

G

S

K

E

Q

K

E

Y

F

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

I

D

D

K

Q

L

A

A

R

F

F

T

V

A

G

K

-

L

L

K

K

S

D

T

I

S

T

G

-

L

-

D

-

I

-

G

G

A

G

S

C

M

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

S

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

K

R

H

H

C

C

E

E

N

N

A

A

M

L

K

K

I

I

A

A

S

R

Y

F

L

L

E

E

K

G

S

H

D

P

K

S

V

I

T

T

N

R

V

V

N

Y

Y

Y

P

P

N

G

L

L

K

S

S

S

S

H

K

P

Y

Q

Y

Y

D

E

L

L

A

G

T

Q

K

R

Y

Q

Y

M

A

S

D

L

G

-

A

P

S

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

T

G

K

L

E

E

N

A

A

G

Y

R

K

R

F

M

L

I

A

N

K

S

L

V

K

E

L

L

I

C

L

L

D

L

T

A

T

V

N

S

I

L

G

G

D

D

C

T

K

E

T

T

I

L

I

I

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

P

N

V

T

A

E

P

E

E

E

E

R

R

E

L

K

K

M

A

A

G

V

I

Y

T

D

D

D

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

P

E

E

D

D

I

I

L

I

E

N

D

D

I

L

D

E

R

H

A

A

L

I

E

R

active site: Y101 (= Y106), D173 (= D177), K198 (= K202)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G81), I77 (≠ M82), Y101 (= Y106), E144 (= E148), D173 (= D177), F176 (≠ I180), S195 (= S199), T197 (≠ S201), K198 (= K202)

8erbK Crystal structure of fub7 in complex with vinylglycine ketimine (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 6:425/429 of 8erbK

query
sites
8erbK

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

Y

Y

N

T

L

P

E

D

-

P

N

H

T

T

G

R

A

S

T

T

N

A

V

V

P

P

I

I

Y

Y

M

A

S

T

N

S

A

S

Y

Y

A

T

H

F

S

N

S

D

P

S

Q

A

E

H

L

G

E

A

N

R

V

L

F

F

K

G

G

L

K

K

F

E

P

L

G

G

Y

N

V

I

Y
|
Y

S

S

R
|
R

F

L

S

M

N

N

P

P

T

T

V

V

L

D

E

V

F

F

E

E

R

K

R

R

M

I

A

A

S

A

I

L

E

E

G

G

G

G

L

I

T

A

A

A

T

A

S

A

A

T

A

S

S

S

G
|
G

M
x
Q

S

A

A

A

I
x
Q

Y

F

M

L

A

T

I

I

T

A

N

T

I

L

V

A

H

K

P

A

G

G

D

D

E

N

I

I

I

V

A

A

S

S

T

S

G

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

T

N

L

Q

I

L

S

N

N

V

-

L

L

L

Q

P

N

R

I

F

G

G

V

I

K

K

V

T

I

K

F

F

L

V

E

R

D

S

I

G

S

K

K

L

K

E

T

D

L

Y

L

A

N

A

N

A

I

I

N

D

E

D

N

Q

T

T

K

R

L

A

V

I

Y

Y

T

V

E

E

T

S

I

M

G

S

N
|
N

P

P

K

D

L

Y

D

V

I

V

L

P

D

D

I

F

E

E

S

G

V

I

G

A

R

K

V

I

C

A

K

H

E

E

K

H

G

G

I

I

I

P

F

L

M

V

V

V

D
|
D

S

N

T

T

I

L

S

G

T

A

-

G

P

G

Y

Y

L

Y

I

I

N

R

P

P

S

I

K

E

Y

H

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

A

S
x
T

K
|
K

Y

W

I

I

N

G

G

G

T

H

S

G

N

T

A

T

I

I

G

G

G

G

M

V

I

I

V

V

D

D

C

S

G

G

S

R

E

F

K

N

Y

W

K

N

N

K

H

H

-

S

-

D

R

R

Y

F

E

P

N

E

F

M

-

V

-

E

-

P

-

S

-

P

-

S

-

Y

-

H

-

G

Q

L

K

K

Y

Y

A

W

E

E

K

A

Y

F

D

G

K

P

L

A

A

T

F

F

T

I

A

T

K

R

L

I

K

R

S

V

T

E

S

M

G

L

L

R

D

D

I

I

G

G

A

A

S

C

M

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

S

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

S

G

L

L

R

R

M

A

K

E

K

R

H

H

C

A

E

Q

N

N

A

T

M

E

K

K

I

L

A

S

S

K

Y

Y

L

F

E

E

K

S

S

S

D

P

K

N

V

V

T

S

N

W

V

V

N

L

Y

W

P

P

N

G

L

S

K

E

S

S

S

H

K

P

Y

T

Y

Y

D

S

L

Q

A

A

T

K

K

K

Y

Y

Y

L

A

T

D

R

G

G

A

F

S

G

G

A

I

M

L

L

T

S

F

-

R

-

L

I

G

G

T

V

K

K

E

G

N

D

A

A

-

S

-

A

-

G

Y

S

K

K

F

V

L

V

A

D

K

G

L

L

K

K

L

L

I

V

L

S

D

N

T

L

T

A

N

N

I

V

G

G

D

D

C

A

K

K

T

S

I

L

I

A

I

I

H

H

P

P

A

W

S

S

T

T

I
x
T

C

H

I

E

N

Q

N

L

T

S

E

E

E

D

E

E

R

R

E

L

K

A

M

S

A

G

V

V

Y

T

D

E

D

D

L

M

L

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

I

V

E

D

D

D

I

I

L

I

E

A

D

D

I

F

D

E

R

Q

A

S

L

F

E

Q

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y55 (= Y51), R57 (= R53), G85 (= G81), Q86 (≠ M82), Q89 (≠ I85), Y110 (= Y106), N157 (= N152), D182 (= D177), S205 (= S199), T207 (≠ S201), K208 (= K202), T385 (≠ I367), R405 (= R387)

8erjB Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 5:424/428 of 8erjB

query
sites
8erjB

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

Y

Y

N

T

L

P

E

D

-

P

N

H

T

T

G

R

A

S

T

T

N

A

V

V

P

P

I

I

Y

Y

M

A

S

T

N

S

A

S

Y

Y

A

T

H

F

S

N

S

D

P

S

Q

A

E

H

L

G

E

A

N

R

V

L

F

F

K

G

G

L

K

K

F

E

P

L

G

G

Y

N

V

I

Y

Y

S

S

R

R

F

L

S

M

N

N

P

P

T

T

V

V

L

D

E

V

F

F

E

E

R

K

R

R

M

I

A

A

S

A

I

L

E

E

G

G

G

G

L

I

T

A

A

A

T

A

S

A

A

T

A

S

S

S

G
|
G

M
x
Q

S

A

A

A

I
x
Q

Y

F

M

L

A

T

I

I

T

A

N

T

I

L

V

A

H

K

P

A

G

G

D

D

E

N

I

I

I

V

A

A

S

S

T

S

G

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

T

N

L

Q

I

L

S

N

N

V

-

L

L

L

Q

P

N

R

I

F

G

G

V

I

K

K

V

T

I

K

F

F

L

V

E

R

D

S

I

G

S

K

K

L

K

E

T

D

L

Y

L

A

N

A

N

A

I

I

N

D

E

D

N

Q

T

T

K

R

L

A

V

I

Y

Y

T

V

E

E

T

S

I

M

G

S

N

N

P

P

K

D

L

Y

D

V

I

V

L

P

D

D

I

F

E

E

S

G

V

I

G

A

R

K

V

I

C

A

K

H

E

E

K

H

G

G

I

I

I

P

F

L

M

V

V

V

D
|
D

S

N

T

T

I

L

S

G

T

A

-

G

P

G

Y

Y

L

Y

I

I

N

R

P

P

S

I

K

E

Y

H

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

A

S

T

K
|
K

Y

W

I

I

N

G

G

G

T

H

S

G

N

T

A

T

I

I

G

G

G

G

M

V

I

I

V

V

D

D

C

S

G

G

S

R

E

F

K

N

Y

W

K

N

N

K

H

H

-

S

-

D

R

R

Y

F

E

P

N

E

F

M

-

V

-

E

-

P

-

S

-

P

-

S

-

Y

-

H

-

G

Q

L

K

K

Y

Y

A

W

E

E

K

A

Y

F

D

G

K

P

L

A

A

T

F

F

T

I

A

T

K

R

L

I

K

R

S

V

T

E

S

M

G

L

L

R

D

D

I

I

G

G

A

A

S

C

M

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

S

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

S

G

L

L

R

R

M

A

K

E

K

R

H

H

C

A

E

Q

N

N

A

T

M

E

K

K

I

L

A

S

S

K

Y

Y

L

F

E

E

K

S

S

S

D

P

K

N

V

V

T

S

N

W

V

V

N

L

Y

W

P

P

N

G

L

S

K

E

S

S

S

H

K

P

Y

T

Y

Y

D

S

L

Q

A

A

T

K

K

K

Y

Y

Y

L

A

T

D

R

G

G

A

F

S

G

G

A

I

M

L

L

T

S

F

-

R

-

L

I

G

G

T

V

K

K

E

G

N

D

A

A

-

S

-

A

-

G

Y

S

K

K

F

V

L

V

A

D

K

G

L

L

K

K

L

L

I

V

L

S

D

N

T

L

T
x
A

N
|
N

I

V

G

G

D

D

C

A

K

K

T

S

I

L

I

A

I

I

H

H

P

P

A

W

S

S

T

T

I
x
T

C

H

I

E

N

Q

N

L

T

S

E

E

E

D

E

E

R

R

E

L

K

A

M

S

A

G

V

V

Y

T

D

E

D

D

L

M

L

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

I

V

E

D

D

D

I

I

L

I

E

A

D

D

I

F

D

E

R

Q

A

S

L

F

E

Q

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]but-3-enoic acid: G84 (= G81), Q85 (≠ M82), Q88 (≠ I85), Y109 (= Y106), D181 (= D177), S204 (= S199), K207 (= K202), A368 (≠ T351), N369 (= N352), T384 (≠ I367), R404 (= R387)

8erjA Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
35% identity, 99% coverage: 3:407/409 of query aligns to 5:424/428 of 8erjA

query
sites
8erjA

F

F

S

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

Y

Y

N

T

L

P

E

D

-

P

N

H

T

T

G

R

A

S

T

T

N

A

V

V

P

P

I

I

Y

Y

M

A

S

T

N

S

A

S

Y

Y

A

T

H

F

S

N

S

D

P

S

Q

A

E

H

L

G

E

A

N

R

V

L

F

F

K

G

G

L

K

K

F

E

P

L

G

G

Y

N

V

I

Y

Y

S

S

R

R

F

L

S

M

N

N

P

P

T

T

V

V

L

D

E

V

F

F

E

E

R

K

R

R

M

I

A

A

S

A

I

L

E

E

G

G

G

G

L

I

T

A

A

A

T

A

S

A

A

T

A

S

S
|
S

G
|
G

M
x
Q

S

A

A

A

I
x
Q

Y

F

M

L

A

T

I

I

T

A

N

T

I

L

V

A

H

K

P

A

G

G

D

D

E

N

I

I

I

V

A

A

S

S

T

S

G

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

T

N

L

Q

I

L

S

N

N

V

-

L

L

L

Q

P

N

R

I

F

G

G

V

I

K

K

V

T

I

K

F

F

L

V

E

R

D

S

I

G

S

K

K

L

K

E

T

D

L

Y

L

A

N

A

N

A

I

I

N

D

E

D

N

Q

T

T

K

R

L

A

V

I

Y

Y

T

V

E

E

T

S

I

M

G

S

N
|
N

P

P

K

D

L

Y

D

V

I

V

L

P

D

D

I

F

E

E

S

G

V

I

G

A

R

K

V

I

C

A

K

H

E

E

K

H

G

G

I

I

I

P

F

L

M

V

V

V

D
|
D

S

N

T

T

I

L

S

G

T

A

-

G

P

G

Y

Y

L

Y

I

I

N

R

P

P

S

I

K

E

Y

H

G

G

A

A

D

D

V

I

I

V

I

V

H

H

S
|
S

T

A

S
x
T

K
|
K

Y

W

I

I

N

G

G

G

T

H

S

G

N

T

A

T

I

I

G

G

G

G

M

V

I

I

V

V

D

D

C

S

G

G

S

R

E

F

K

N

Y

W

K

N

N

K

H

H

-

S

-

D

R

R

Y

F

E

P

N

E

F

M

-

V

-

E

-

P

-

S

-

P

-

S

-

Y

-

H

-

G

Q

L

K

K

Y

Y

A

W

E

E

K

A

Y

F

D

G

K

P

L

A

A

T

F

F

T

I

A

T

K

R

L

I

K

R

S

V

T

E

S

M

G

L

L

R

D

D

I

I

G

G

A

A

S

C

M

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

S

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

S

G

L

L

R

R

M

A

K

E

K

R

H

H

C

A

E

Q

N

N

A

T

M

E

K

K

I

L

A

S

S

K

Y

Y

L

F

E

E

K

S

S

S

D

P

K

N

V

V

T

S

N

W

V

V

N

L

Y

W

P

P

N

G

L

S

K

E

S

S

S

H

K

P

Y

T

Y

Y

D

S

L

Q

A

A

T

K

K

K

Y

Y

Y

L

A

T

D

R

G

G

A

F

S

G

G

A

I

M

L

L

T

S

F

-

R

-

L

I

G

G

T

V

K

K

E

G

N

D

A

A

-

S

-

A

-

G

Y

S

K

K

F

V

L

V

A

D

K

G

L

L

K

K

L

L

I

V

L

S

D

N

T

L

T

A

N

N

I

V

G

G

D

D

C

A

K

K

T

S

I

L

I

A

I

I

H

H

P

P

A

W

S

S

T

T

I

T

C

H

I

E

N

Q

N

L

T

S

E

E

E

D

E

E

R

R

E

L

K

A

M

S

A

G

V

V

Y

T

D

E

D

D

L

M

L

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

I

V

E

D

D

D

I

I

L

I

E

A

D

D

I

F

D

E

R

Q

A

S

L

F

E

Q

binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: S83 (= S80), G84 (= G81), Q85 (≠ M82), Q88 (≠ I85), Y109 (= Y106), N156 (= N152), D181 (= D177), S204 (= S199), T206 (≠ S201), K207 (= K202), R404 (= R387)

3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
33% identity, 99% coverage: 3:408/409 of query aligns to 9:395/397 of 3vk3A

query
sites
3vk3A

F

F

S

A

T

T

K

R

L

A

I

I

H

H

G

H

N

G

Y

Y

N

D

L

P

E

Q

N

D

T

H

G

G

A

G

T

A

N

L

V

V

P

P

-

P

I

V

Y

Y

M

Q

S

T

N

A

A

T

Y

F

A

T

H

F

S

P

S

T

P

V

Q

E

E

Y

L

G

E

A

N

A

V

C

F

F

K

A

G

G

K

E

F

Q

P

A

G

G

Y

H

V

F

Y

Y

S

S

R
|
R

F

I

S

S

N

N

P

P

T

T

V

L

L

N

E

L

F

L

E

E

R

A

R

R

M

M

A

A

S

S

I

L

E

E

G

G

G

G

L

E

T

A

A

G

T

L

S

A

A

L

A

A

S

S

G

G

M

M

S

G

A

A

I

I

Y

T

M

S

A

T

I

L

T

W

N

T

I

L

V

L

H

R

P

P

G

G

D

D

E

E

I

V

I

L

A

L

S

G

T

N

G

T

L

L

Y
|
Y

G

G

G

H

T

T

Y

F

T

A

L

F

I

L

S

H

N

H

-

G

L

I

Q

G

N

E

I

F

G

G

V

V

K

K

V

L

I

R

F

H

L

V

E

D

D

M

I

A

S

D

K

L

K

Q

T

A

L

L

L

E

N

A

N

A

I

M

N

T

E

P

N

A

T

T

K

R

L

V

V

I

Y

Y

T

F

E

E

T

S

I

P

G

A

N

N

P

P

K

N

L

M

D

H

I

M

L

A

D

D

I

I

E

A

S

G

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V

G

A

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I

C

A

K

R

E

K

K

H

G

G

I

A

I

T

F

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M

V

V

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D
|
D

S

N

T

T

I

Y

S

C

T

T

P

P

Y

Y

L

L

I

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N

R

P

P

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L

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E

Y

L

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G

A

A

D

D

V

L

I

V

I

V

H

H

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|
K

Y

Y

I

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N

S

G

G

T

H

S

G

N

D

A

I

I

T

G

A

G

G

M

I

I

V

V

V

D

-

C

-

G

G

S

S

E

Q

K

A

Y

L

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

V

D

D

K

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L

I

A

R

F

L

T

Q

A

G

K

-

L

L

K

K

S

D

T

M

S

T

G

-

L

-

D

-

I

-

G

G

A

A

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V

M

L

S

S

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P

F

H

N

D

A

A

F

A

L

L

S

L

L

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T

R

G

G

I

I

E

K

T

T

L

L

S

N

L

L

R

R

M

M

K

D

K

R

H

H

C

C

E

A

N

N

A

A

M

Q

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I

L

A

A

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E

Y

F

L

L

E

A

K

R

S

Q

D

P

K

Q

V

V

T

E

N

L

V

I

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H

Y

Y

P

P

N

G

L

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A

S

S

S

F

K

P

Y

Q

Y

Y

D

T

L

L

A

A

T

R

K

Q

Y

Q

Y

M

A

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D

Q

G

-

A

P

S

G

G

G

I

M

L

I

T

A

F

F

R

E

L

L

-

K

G

G

T

G

K

I

E

G

N

A

A

G

Y

R

K

R

F

F

L

M

A

N

K

A

L

L

K

Q

L

L

I

F

L

S

D

R

T

A

T
x
V

N
x
S

I
x
L

G

G

D

D

C

A

K

E

T

S

I

L

I

A

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

S

N

Y

T

T

E

P

E

E

E

E

R

R

E

A

K

H

M

Y

A

G

V

I

Y

S

D

E

D

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

I

E

D

D

D

I

L

L

L

E

A

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D

I

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D

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Q

A

A

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L

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A

A

active site: R60 (= R53), Y113 (= Y106), D185 (= D177), K210 (= K202)
binding methionine: Y113 (= Y106), K210 (= K202), V338 (≠ T351), S339 (≠ N352), L340 (≠ I353), R374 (= R387)

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
33% identity, 99% coverage: 3:408/409 of query aligns to 5:391/393 of 5x30C

query
sites
5x30C

F

F

S

A

T

T

K

R

L

A

I

I

H

H

G

H

N

G

Y

Y

N

D

L

P

E

Q

N

D

T

H

G

G

A

G

T

A

N

L

V

V

P

P

-

P

I

V

Y

Y

M

Q

S

T

N

A

A

T

Y

F

A

T

H

F

S

P

S

T

P

V

Q

E

E

Y

L

G

E

A

N

A

V

C

F

F

K

A

G

G

K

E

F

Q

P

A

G

G

Y

H

V

F

Y
|
Y

S

S

R
|
R

F

I

S

S

N

N

P

P

T

T

V

L

L

N

E

L

F

L

E

E

R

A

R

R

M

M

A

A

S

S

I

L

E

E

G

G

G

G

L

E

T

A

A

G

T

L

S

A

A

L

A

A

S

S

G

G

M

M

S

G

A

A

I

I

Y

T

M

S

A

T

I

L

T

W

N

T

I

L

V

L

H

R

P

P

G

G

D

D

E

E

I

V

I

L

A

L

S

G

T

N

G

T

L

L

Y
|
Y

G

G

G

H

T

T

Y

F

T

A

L

F

I

L

S

H

N

H

-

G

L

I

Q

G

N

E

I

F

G

G

V

V

K

K

V

L

I

R

F

H

L

V

E

D

D

M

I

A

S

D

K

L

K

Q

T

A

L

L

L

E

N

A

N

A

I

M

N

T

E

P

N

A

T

T

K

R

L

V

V

I

Y

Y

T

F

E

E

T

S

I

P

G

A

N

N

P

P

K

N

L

M

D

H

I

M

L

A

D

D

I

I

E

A

S

G

V

V

G

A

R

K

V

I

C

A

K

R

E

K

K

H

G

G

I

A

I

T

F

V

M

V

V

V

D
|
D

S

N

T

T

I

Y

S

C

T

T

P

P

Y

Y

L

L

I

Q

N

R

P

P

S

L

K

E

Y

L

G

G

A

A

D

D

V

L

I

V

I

V

H

H

S

S

T

A

S

T

K
|
K

Y

Y

I

L

N

S

G

G

T

H

S

G

N

D

A

I

I

T

G

A

G

G

M

I

I

V

V

V

D

-

C

-

G

G

S

S

E

Q

K

A

Y

L

K

-

N

-

H

-

R

-

Y

-

E

-

N

-

F

-

Q

-

K

-

Y

-

A

-

E

-

K

-

Y

V

D

D

K

R

L

I

A

R

F

L

T

Q

A

G

K

-

L

L

K

K

S

D

T

M

S

T

G

-

L

-

D

-

I

-

G

G

A

A

S

V

M

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

S

L

L

M

T

R

G

G

I

I

E

K

T

T

L

L

S

N

L

L

R

R

M

M

K

D

K

R

H

H

C

C

E

A

N

N

A

A

M

Q

K

V

I

L

A

A

S

E

Y

F

L

L

E

A

K

R

S

Q

D

P

K

Q

V

V

T

E

N

L

V

I

N

H

Y

Y

P

P

N

G

L

L

K

A

S

S

S

F

K

P

Y

Q

Y

Y

D

T

L

L

A

A

T

R

K

Q

Y

Q

Y

M

A

S

D

Q

G

-

A

P

S

G

G

G

I

M

L

I

T

A

F

F

R

E

L

L

-

K

G

G

T

G

K

I

E

G

N

A

A

G

Y

R

K

R

F

F

L

M

A

N

K

A

L

L

K

Q

L

L

I

F

L

S

D

R

T

A

T

V

N

S

I

L

G

G

D

D

C

A

K

E

T

S

I

L

I

A

I

Q

H

H

P

P

A

A

S

S

T

M

I

T

C

H

I

S

N

S

N

Y

T

T

E

P

E

E

E

E

R

R

E

A

K

H

M

Y

A

G

V

I

Y

S

D

E

D

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

I

I

E

D

D

D

I

L

L

L

E

A

D

D

I

V

D

Q

R

Q

A

A

L

L

E

K

A

A

active site: R56 (= R53), Y109 (= Y106), D181 (= D177), K206 (= K202)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y51), R56 (= R53)

Query Sequence

>WP_010963429.1 NCBI__GCF_000008765.1:WP_010963429.1
MKFSTKLIHGNYNLENTGATNVPIYMSNAYAHSSPQELENVFKGKFPGYVYSRFSNPTVL
EFERRMASIEGGLTATSAASGMSAIYMAITNIVHPGDEIIASTGLYGGTYTLISNLQNIG
VKVIFLEDISKKTLLNNINENTKLVYTETIGNPKLDILDIESVGRVCKEKGIIFMVDSTI
STPYLINPSKYGADVIIHSTSKYINGTSNAIGGMIVDCGSEKYKNHRYENFQKYAEKYDK
LAFTAKLKSTSGLDIGASMSPFNAFLSLTGIETLSLRMKKHCENAMKIASYLEKSDKVTN
VNYPNLKSSKYYDLATKYYADGASGILTFRLGTKENAYKFLAKLKLILDTTNIGDCKTII
IHPASTICINNTEEEREKMAVYDDLLRLSVGIEDIEDILEDIDRALEAI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory