SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964029.1 NCBI__GCF_000008765.1:WP_010964029.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
61% identity, 99% coverage: 3:397/397 of query aligns to 1:397/654 of P36204

query
sites
P36204

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R36 (= R38) binding substrate
R118 (= R121) binding substrate
R151 (= R154) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
61% identity, 99% coverage: 4:397/397 of query aligns to 1:396/398 of 1vpeA

query
sites
1vpeA

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active site: R35 (= R38), K196 (= K201), G353 (= G354), G376 (= G377)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G199), A195 (= A200), K196 (= K201), K200 (= K205), G218 (= G223), A219 (≠ G224), N316 (= N320), P318 (= P322), G320 (= G324), V321 (= V325), E323 (= E327), G352 (= G353), G353 (= G354), D354 (= D355), S355 (= S356)

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
58% identity, 99% coverage: 3:397/397 of query aligns to 1:394/394 of P40924

query
sites
P40924

F

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S183 (≠ E187) modified: Phosphoserine
T299 (= T303) modified: Phosphothreonine

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
58% identity, 99% coverage: 3:397/397 of query aligns to 1:394/394 of 1phpA

query
sites
1phpA

F

M

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-

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K

D

T

D

N

T

E

V

A

V

V

G

G

E

D

N

E

A

V

K

K

K

A

A

A

V

V

A

D

E

R

L

L

K

N

E

E

G

G

E

D

I

V

V

L

L

L

E

E

N

N

T

V

R

R

F

F

R

Y

P

P

E

G

E

E

G

E

K

K

N

N

D

D

D

P

A

E

F

L

S

A

K

K

D

A

L

F

A

A

S

E

L

L

A

A

D

D

V

L

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

V

E

G

G

V

I

T

A

K

H

F

Y

V

L

D

-

T

P

A

A

V

V

C

A

G

G

Y

F

L

L

I

M

Q

E

K

K

E

E

L

L

K

E

F

V

L

L

G

G

S

K

A

A

V

L

E

S

N

N

P

P

V

D

R

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G
|
G

A

A

K
|
K

V

V

S

K

D

D

K
|
K

I

I

N

G

V

V

I

I

N

D

N

N

L

L

E

E

K

K

V

V

D

D

T

N

L

L

I

I

G

G

G
|
G

M

L

A

A

Y

Y

T

T

F

F

L

V

K

K

A

A

Q

L

G

G

Y

H

T

D

I

V

G

G

T

K

S

S

L
|
L

L

L

E

E

A

E

D

D

K

K

V

I

D

E

Y

L

A

A

K

K

E

S

M

F

I

M

E

E

K

K

A

A

E

K

A

E

K

K

G

G

V

V

K

R

L

F

L

Y

L

M

P

P

I

V

D

D

N

V

I

V

V

V

G

A

A

D

E

R

F

F

K

A

A

N

D

D

T

A

K

N

A

T

V

K

T

V

E

P

D

I

A

D

N

A

I

I

P

P

E

A

G

D

Y

W

M

S

G

A

L

L

D

D

I

I

G

G

P

P

K

K

T

T

Q

R

K

E

L

L

Y

Y

A

R

D

D

A

V

V

I

K

R

E

E

A

S

K

K

T

L

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

E

D

N

A

F

F

A

A

K

H

G

G

T

T

K

K

-

A

D

I

V

A

A

E

K

A

A

L

M

A

A

E

E

A

S

L

D

D

A

T

T

Y

T

S

V

V

I

I

G

G

G
|
G

D
|
D

S
|
S

A

A

V

V

N

E

Q

K

L

F

G

G

F

L

G

A

D

D

K

K

M

M

T

D

H

H

I

I

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

G

G

I

V

V

V

A

A

L

L

N

E

D

D

K

K

active site: R36 (= R38), K197 (= K201), G351 (= G354), G374 (= G377)
binding adenosine-5'-diphosphate: G195 (= G199), K201 (= K205), G219 (= G223), G220 (= G224), L237 (= L241), N316 (= N320), P318 (= P322), G320 (= G324), V321 (= V325), E323 (= E327), G350 (= G353), D352 (= D355), S353 (= S356)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
58% identity, 99% coverage: 3:397/397 of query aligns to 1:394/394 of P18912

query
sites
P18912

F

M

N

N

K

K

T

T

I

I

E

R

D

D

V

V

D

D

V

V

K

R

G

G

K

K

R

R

V

V

L

F

V

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

E

D

Q

G

G

V

A

I

I

T

T

D

D

E

D

N

T

R

R

L

I

N

R

G

A

A

A

L

L

P

P

T

T

I

I

K

R

Y

Y

L

L

S

I

E

E

H

H

G

G

G

A

K

K

V

V

I

I

L

L

C

A

S

S

H

H

L

L

G

G

K

R

A

P

K

K

G

G

P

K

D

V

P

V

S

E

K

E

-

L

T

R

L

L

A

D

P

A

V

V

A

A

K

K

R

R

L

L

S

G

E

E

L

L

G

E

R

R

E

P

V

V

K

-

F

-

A

A

A

K

D

T

D

N

T

E

V

A

V

V

G

G

E

D

N

E

A

V

K

K

K

A

A

A

V

V

A

D

E

R

L

L

K

N

E

E

G

G

E

D

I

V

V

L

L

L

E

E

N

N

T

V

R

R

F

F

R

Y

P

P

E

G

E

E

G

E

K

K

N

N

D

D

D

P

A

E

F

L

S

A

K

K

D

A

L

F

A

A

S

E

L

L

A

A

D

D

V

L

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

V

E

G

G

V

I

T

A

K

H

F

Y

V

L

D

-

T

P

A

A

V

V

C

A

G

G

Y

F

L

L

I

M

Q

E

K

K

E

E

L

L

K

E

F

V

L

L

G

G

S

K

A

A

V

L

E

S

N

N

P

P

V

D

R

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

A

A

K

K

V

V

S

K

D

D

K
|
K

I

I

N

G

V

V

I

I

N

D

N

N

L

L

E

E

K

K

V

V

D

D

T

N

L

L

I

I

G

G

M

L

A

A

Y

Y

T

T

F

F

L

V

K

K

A

A

Q

L

G

G

Y

H

T

D

I

V

G

G

T

K

S

S

L

L

E

E

A

E

D

D

K

K

V

I

D

E

Y

L

A

A

K

K

E

S

M

F

I

M

E

E

K

K

A

A

E

K

A

E

K

K

G

G

V

V

K

R

L

F

L

Y

L

M

P

P

I

V

D

D

N

V

I

V

V

V

G

A

A

D

E

R

F

F

K

A

A

N

D

D

T

A

K

N

A

T

V

K

T

V

E

P

D

I

A

D

N

A

I

I

P

P

E

A

G

D

Y

W

M

S

G

A

L

L

D

D

I

I

G

G

P

P

K

K

T

T

Q

R

K

E

L

L

Y

Y

A

R

D

D

A

V

V

I

K

R

E

E

A

S

K

K

T

L

V

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

F

M

E

D

N

A

F

F

A

A

K

H

G

G

T

T

K

K

-

A

D

I

V

A

A

E

K

A

A

L

M

A

A

E

E

A

S

L

D

D

A

T

T

Y

T

S

V

V

I

I

G

G

G
|
G

D
|
D

S
|
S

A

A

V

V

N

E

Q

K

L

F

G

G

F

L

G

A

D

D

K

K

M

M

T

D

H

H

I

I

S

S

T

T

G

G

A

A

S

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

G

G

I

V

V

V

A

A

L

L

N

E

D

D

K

K

K201 (= K205) binding ATP
N316 (= N320) binding ATP
E323 (= E327) binding ATP
GGDS 350:353 (= GGDS 353:356) binding ATP

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
49% identity, 99% coverage: 5:397/397 of query aligns to 2:415/415 of 16pkA

query
sites
16pkA

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

K

K

D

N

G

G

V

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

N

R

G

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

S

L

E

T

H

E

G

G

K

S

V

C

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

K

G

G

P

I

D

P

P

M

S

A

K

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

-

K

-

A

T

T

L

L

A

K

P

P

V

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

G

L

R

R

E

P

V

V

K

T

F

F

A

A

A

P

D

D

D

C

T

L

V

-

G

-

E

-

N

N

A

A

K

A

K

D

A

V

V

V

A

S

E

K

L

M

K

S

E

P

G

G

E

D

I

V

V

V

L

L

E

E

N

N

T

V

R

R

F

F

R

Y

P

K

E

E

G

G

-

S

-

K

-

A

K

K

N

D

D

R

D

E

A

A

F

M

S

A

K

K

D

I

L

L

A

A

S

S

L

Y

A

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

V

L

D

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

K

S

F

Y

L

F

G

A

S

K

A

V

V

L

E

G

N

N

P

P

V

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G
x
A

M

M

A

A

Y
|
Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

L

K

L

C

E

E

A

E

D

S

K

K

V

L

D

E

Y

F

A

A

K

R

E

S

M

L

I

L

E

K

K

K

A

A

E

E

A

D

K

R

G

K

V

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

N

H

I

V

V

C

G

H

A

T

E

E

F

F

K

K

A

A

D

V

T

D

K

S

A

P

V

L

T

I

T

T

E

E

D

D

A

Q

N

N

I

I

P

P

E

E

G

G

Y

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

K

K

T

T

Q

I

K

E

L

K

Y

Y

A

V

D

Q

A

T

V

I

K

G

E

K

A

C

K

K

T

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

E

V

N

P

F

Y

A

S

K

K

G

G

T

T

K

F

D

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

D

G

A

L

T

M

T

S

V

I

I

I

G

G

G
|
G

D

D

S

S

A

A

A

S

A

A

V

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

V

T

A

V

L

L

N

D

D

D

K

K

active site: R35 (= R38), K215 (= K201), G372 (= G354), G395 (= G377)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G199), A214 (= A200), K219 (= K205), A238 (≠ G224), Y241 (= Y227), L311 (= L297), P336 (= P322), G338 (= G324), V339 (= V325), E341 (= E327), G393 (= G375), G394 (= G376), G395 (= G377)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
49% identity, 99% coverage: 5:397/397 of query aligns to 2:415/415 of 13pkA

query
sites
13pkA

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

K

K

D

N

G

G

V

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

N

R

G

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

S

L

E

T

H

E

G

G

K

S

V

C

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

K

G

G

P

I

D

P

P

M

S

A

K

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

-

K

-

A

T

T

L

L

A

K

P

P

V

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

G

L

R

R

E

P

V

V

K

T

F

F

A

A

A

P

D

D

D

C

T

L

V

-

G

-

E

-

N

N

A

A

K

A

K

D

A

V

V

V

A

S

E

K

L

M

K

S

E

P

G

G

E

D

I

V

V

V

L

L

E

E

N

N

T

V

R

R

F

F

R

Y

P

K

E

E

G

G

-

S

-

K

-

A

K

K

N

D

D

R

D

E

A

A

F

M

S

A

K

K

D

I

L

L

A

A

S

S

L

Y

A

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

V

L

D

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

K

S

F

Y

L

F

G

A

S

K

A

V

V

L

E

G

N

N

P

P

V

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G

A

M

M

A

A

Y

Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

L

K

L

C

E

E

A

E

D

S

K

K

V

L

D

E

Y

F

A

A

K

R

E

S

M

L

I

L

E

K

K

K

A

A

E

E

A

D

K

R

G

K

V

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

N

H

I

V

V

C

G

H

A

T

E

E

F

F

K

K

A

A

D

V

T

D

K

S

A

P

V

L

T

I

T

T

E

E

D

D

A

Q

N

N

I

I

P

P

E

E

G

G

Y

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

K

K

T

T

Q

I

K

E

L

K

Y

Y

A

V

D

Q

A

T

V

I

K

G

E

K

A

C

K

K

T

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

E

V

N

P

F

Y

A

S

K

K

G

G

T

T

K

F

D

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

D

G

A

L

T

M

T

S

V

I

I

I

G

G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

V

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

V

T

A

V

L

L

N

D

D

D

K

K

active site: R35 (= R38), K215 (= K201), G372 (= G354), G395 (= G377)
binding adenosine-5'-diphosphate: G213 (= G199), A214 (= A200), K219 (= K205), L311 (= L297), P336 (= P322), G338 (= G324), V339 (= V325), E341 (= E327), G371 (= G353), D373 (= D355), S374 (= S356)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
49% identity, 100% coverage: 1:397/397 of query aligns to 1:419/440 of P07378

query
sites
P07378

M

M

K

T

F

L

N

N

-

E

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

V

K

K

D

N

G

G

V

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

N

R

G

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

S

L

E

T

H

E

G

G

K

S

V

C

I

V

L

L

C

M

S

S

H
|
H

L
|
L

G
|
G

K
x
R

A

P

K

K

G

G

P

I

D

P

P

M

S

A

K

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

-

K

-

A

T

T

L

L

A

K

P

P

V

V

A

A

K

K

R

A

L

L

S

S

E

E

L

L

G

L

R

R

E

P

V

V

K

T

F

F

A

A

A

P

D

D

D

C

T

L

V

-

G

-

E

-

N

N

A

A

K

A

K

D

A

V

V

V

A

S

E

K

L

M

K

S

E

P

G

G

E

D

I

V

V

V

L

L

E

E

N

N

T

V

R
|
R

F

F

R

Y

P

K

E

E

G

G

-

S

-

K

-

A

K

K

N

D

D

R

D

E

A

A

F

M

S

A

K

K

D

I

L

L

A

A

S

S

L

Y

A

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

V

L

D

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

K

S

F

Y

L

F

G

A

S

K

A

V

V

L

E

G

N

N

P

P

V

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

A

A

K

K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G

A

M

M

A

A

Y

Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

L

K

L

C

E

E

A

E

D

S

K

K

V

L

D

E

Y

F

A

A

K

R

E

S

M

L

I

L

E

K

K

K

A

A

E

E

A

D

K

R

G

K

V

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

N

H

I

V

V

C

G

H

A

T

E

E

F

F

K

K

A

A

D

V

T

D

K

S

A

P

V

L

T

I

T

T

E

E

D

D

A

Q

N

N

I

I

P

P

E

E

G

G

Y

H

M

M

G

A

L

L

D

D

I

I

G

G

P

P

K

K

T

T

Q

I

K

E

L

K

Y

Y

A

V

D

Q

A

T

V

I

K

G

E

K

A

C

K

K

T

S

V

A

V

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

F

M

E

V

N

P

F

Y

A

S

K

K

G

G

T

T

K

F

D

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

D

G

A

L

T

M

T

S

V

I

I

I

G

G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

V

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

V

T

A

V

L

L

N

D

D

E

K

K

DFN 24:26 (= DFN 23:25) binding substrate
R39 (= R38) binding substrate
HLGR 62:65 (≠ HLGK 61:64) binding substrate
R135 (= R121) binding substrate
R172 (= R154) binding substrate
K223 (= K205) binding ATP
N338 (= N320) binding ATP
E345 (= E327) binding ATP
GGDS 375:378 (= GGDS 353:356) binding ATP

3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
54% identity, 99% coverage: 5:397/397 of query aligns to 3:398/398 of 3zlbA

query
sites
3zlbA

K

K

K

L

T

T

I

V

E

K

D
|
D

V

V

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

V

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

K

K

D

D

G

G

V

V

I

I

T

T

D

N

E

D

N

N

R
|
R

L

I

N

T

G

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

I

S

I

E

E

H

Q

G

G

K

R

V

A

I

I

L

L

C

F

S

S

H

H

L

L

G

G

K

R

A

V

K

K

-

E

G

S

P

D

D

K

P

A

S

G

K

K

T

S

L

L

A

A

P

P

V

V

A

A

K

A

R

D

L

L

S

A

E

A

L

K

L

L

G

G

R

Q

E

D

V

V

K

V

F

F

A

P

A

G

D

-

T

V

V

T

V

R

G

G

E

A

N

E

A

L

K

E

K

A

A

A

V

I

A

N

E

A

L

L

K

E

E

D

G

G

E

Q

I

V

V

L

L

L

L

V

E

E

N

N

T

T

R

R

F

Y

R

E

-

D

-

V

-

D

-

G

P

K

E

K

E

E

G

S

K

K

N

N

D

D

D

P

A

E

F

L

S

G

K

K

D

Y

L

W

A

A

S

S

L

L

A

G

D

D

-

G

V

I

Y

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

N

V

V

G

G

V

I

T

S

K

A

F

N

V

V

D

E

T

K

A

A

V

V

C

A

G

G

Y

F

L

L

I

L

Q

E

K

N

E

E

L

I

K

A

F

Y

L

I

G

Q

S

E

A

A

V

V

E

E

N

T

P

P

V

E

R

R

P

P

F

F

V

V

A

A

I

I

L

L

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

D

D

K

K

I

I

N

G

V

V

I

I

N

E

N

N

L

L

E

E

K

K

V

A

D

D

T

K

L

V

I

L

I

I

G

G

G
|
G

M

M

A

T

Y

Y

T

T

F

F

L

Y

K

K

A

A

Q

Q

G

G

Y

I

T

E

I

I

G

G

T

N

S

S

L

L

L

V

E

E

A

E

D

D

K

K

V

L

D

D

Y

V

A

A

K

K

E

A

M

L

I

L

E

E

K

K

A

A

E

N

A

G

K

-

G

-

V

-

K

K

L

L

L

I

L

L

P

P

I

V

D

D

N

S

I

K

V

E

G

A

A

N

E

A

F

F

K

A

A

G

D

Y

T

T

K

E

A

V

V

R

T

D

T

T

E

E

D

G

A

E

N

A

I

V

P

S

E

E

G

G

Y

F

M

L

G

G

L

L

D

D

I

I

G

G

P

P

K

K

T

S

Q

I

K

A

L

K

Y

F

A

D

D

E

A

A

V

L

K

T

E

G

A

A

K

K

T

T

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

N

E

P

N

D

F

F

A

Q

K

A

G

G

T

T

K

I

D

G

V

V

A

M

K

D

A

A

M

I

A

V

E

K

S

Q

D

P

A

G

T

V

-

K

T

S

V

I

I

I

G

G

G
|
G

D
|
D

S
|
S

A

A

V

A

N

I

Q

N

L

L

G

G

F

R

G

A

D

D

K

K

M

F

T

S

H

W

I

I

S

S

T

T

G

G

G
|
G

A

A

S

S

L

M

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

L

V

A

A

A

L

L

N

T

D

E

K
|
K

active site: R36 (= R38), K204 (= K201), G355 (= G354), G378 (= G377)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G199), S203 (≠ A200), G226 (= G223), G227 (= G224), N320 (= N320), P322 (= P322), G324 (= G324), V325 (= V325), E327 (= E327), G354 (= G353), G355 (= G354), D356 (= D355), S357 (= S356)
binding magnesium ion: D8 (= D10), K398 (= K397)

Sites not aligning to the query:

binding magnesium ion: 1

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
50% identity, 99% coverage: 4:397/397 of query aligns to 5:415/416 of P00560

query
sites
P00560

N

S

K

K

K

L

T

S

I

V

E

Q

D

D

V

L

D

D

V

L

K

K

G

D

K

K

R

R

V

V

L

F

V

I

R
|
R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

K

D

D

G

G

K

V

K

I

I

T

T

D

S

E

N

N

Q

R
|
R

L

I

N

V

G

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

V

S

L

E

E

H

H

G

H

G

P

K

R

-

Y

V

V

I

V

L

L

C

A

S

S

H

H

L

L

G

G

K

R

A

P

K

N

G

G

P

E

D

R

P

N

S

E

K

K

-

Y

T

S

L

L

A

A

P

P

V

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

G

G

R

K

E

D

V

V

K

T

F

F

A

L

A

N

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

A

A

A

V

V

A

K

E

A

L

S

K

A

E

P

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R
|
R

F

Y

R

H

P

I

E

E

-

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

G

S

K

K

N

E

D

D

-

V

D

Q

A

K

F

F

S

R

K

H

D

E

L

L

A

S

S

S

L

L

A

A

D

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

F

T

D

K

-

F

-

V

L

D

P

T

Q

A

R

V

A

C

A

G

G

Y

F

L

L

I

L

Q

E

K

K

E

E

L

L

K

K

F

Y

L

F

G

G

S

K

A

A

V

L

E

E

N

N

P

P

V

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

A

A

K

K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

E

D

K

K

V

V

D

D

T

S

L

I

I

I

G

G

M

M

A

A

Y

F

T

T

F

F

L

K

K

K

A

V

-

L

Q

E

G

N

Y

T

T

E

I

I

G

G

T

D

S

S

L

I

L

F

E

D

A

K

D

A

K

G

V

A

D

E

Y

I

A

V

K

P

E

K

M

L

I

M

E

E

K

K

A

A

E

K

A

A

K

K

G

G

V

V

K

E

L

V

L

L

P

P

I

V

D

D

N

F

I

I

V

I

G

A

A

D

E

A

F

F

K

S

A

A

D

D

-

A

-

N

T

T

K

K

A

T

V

V

T

T

T

D

E

K

D

E

A

G

N

-

I

I

P

P

E

A

G

G

Y

W

M

Q

G

G

L

L

D

D

I

N

G

G

P

P

K

E

T

S

Q

R

K

K

L

L

Y

F

A

A

D

A

A

T

V

V

K

A

E

K

A

A

K

K

T

T

V

I

V

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

F

F

E

E

N

K

F

F

A

A

K

A

G

G

T

T

K

K

D

A

V

L

A

L

K

D

A

E

M

V

A

V

E

K

S

S

D

S

A

A

-

A

-

G

-

N

T

T

T

V

V

I

I

I

G

G

D

D

S

T

A

A

A

T

A

V

V

A

N

K

Q

K

L

Y

G

G

F

V

G

T

D

D

K

K

M

I

T

S

H

H

I

V

S

S

T

T

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

V

A

A

F

L

L

N

S

D

E

K

K

R22 (= R21) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R38) binding substrate
R122 (= R121) binding substrate
R169 (= R154) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
50% identity, 99% coverage: 4:397/397 of query aligns to 4:414/415 of 1qpgA

query
sites
1qpgA

N

S

K

K

K

L

T

S

I

V

E

Q

D

D

V

L

D

D

V

L

K

K

G

D

K

K

R

R

V

V

L

F

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

K

D

D

G

G

K

V

K

I

I

T

T

D

S

E

N

N

Q

R
|
R

L

I

N

V

G

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

V

S

L

E

E

H

H

G

H

G

P

K

R

-

Y

V

V

I

V

L

L

C

A

S

S

H

H

L

L

G

G

K

Q

A

P

K

N

G

G

P

E

D

R

P

N

S

E

K

K

-

Y

T

S

L

L

A

A

P

P

V

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

G

G

R

K

E

D

V

V

K

T

F

F

A

L

A

N

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

A

A

A

V

V

A

K

E

A

L

S

K

A

E

P

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

Y

R

H

P

I

E

E

-

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

G

S

K

K

N

E

D

D

-

V

D

Q

A

K

F

F

S

R

K

H

D

E

L

L

A

S

S

S

L

L

A

A

D

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

F

T

D

K

-

F

-

V

L

D

P

T

Q

A

R

V

A

C

A

G

G

Y

F

L

L

I

L

Q

E

K

K

E

E

L

L

K

K

F

Y

L

F

G

G

S

K

A

A

V

L

E

E

N

N

P

P

V

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

A

A

K
|
K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

E

D

K

K

V

V

D

D

T

S

L

I

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

K

K

K

A

V

-

L

Q

E

G

N

Y

T

T

E

I

I

G

G

T

D

S

S

L

I

L

F

E

D

A

K

D

A

K

G

V

A

D

E

Y

I

A

V

K

P

E

K

M

L

I

M

E

E

K

K

A

A

E

K

A

A

K

K

G

G

V

V

K

E

L

V

L

L

P

P

I

V

D

D

N

F

I

I

V

I

G

A

A

D

E

A

F

F

K

S

A

A

D

D

-

A

-

N

T

T

K

K

A

T

V

V

T

T

T

D

E

K

D

E

A

G

N

-

I

I

P

P

E

A

G

G

Y

W

M

Q

G

G

L

L

D

D

I

N

G

G

P

P

K

E

T

S

Q

R

K

K

L

L

Y

F

A

A

D

A

A

T

V

V

K

A

E

K

A

A

K

K

T

T

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

F
|
F

E
|
E

F

F

E

E

N

K

F

F

A

A

K

A

G

G

T

T

K

K

D

A

V

L

A

L

K

D

A

E

M

V

A

V

E

K

S

S

D

S

A

A

-

A

-

G

-

N

T

T

T

V

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

V

V

A

N

K

Q

K

L

Y

G

G

F

V

G

T

D

D

K

K

M

I

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

V

A

A

F

L

L

N

S

D

E

K

K

active site: R38 (= R38), K213 (= K201), G371 (= G354), G394 (= G377)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G223), G236 (= G224), N334 (= N320), P336 (= P322), G338 (= G324), V339 (= V325), F340 (= F326), E341 (= E327), G370 (= G353), G371 (= G354), D372 (= D355), T373 (≠ S356)

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
49% identity, 98% coverage: 7:397/397 of query aligns to 5:389/392 of 4feyA

query
sites
4feyA

T

T

I

L

E

K

D

D

V

V

D

D

V

L

K

K

G

D

K

K

R

K

V

V

L

L

V

V

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

K

K

D

D

G

G

V

K

I

V

T

T

D

S

E

K

N

V

R
|
R

L

I

N

E

G

A

A

A

L

I

P

P

T

T

I

I

K

Q

Y

Y

L

I

S

L

E

D

H

Q

G

G

K

A

V

V

I

I

L

L

C

M

S

S

H

H

L

L

G

G

K

R

-

P

A

T

K

E

G

G

P

E

-

Y

D

D

P

S

S

Q

K

F

T

S

L

L

A

E

P

P

V

V

A

A

K

K

R

A

L

L

S

S

E

E

L

I

G

N

R

K

E

P

V

V

K

K

F

F

A

A

A

K

D

D

-

W

-

L

D

D

T

G

V

V

V

-

G

-

E

-

N

-

A

-

K

-

A

-

V

-

A

-

E

D

L

V

K

K

E

A

G

G

E

E

I

I

V

V

L

M

L

C

E

E

N

N

T

V

R

R

F

F

R

N

P

S

E

G

E

E

G

K

K

K

N

S

D

T

D

D

A

D

F

L

S

S

K

K

D

K

L

I

A

A

S

S

L

L

A

G

D

D

V

V

Y

F

V

V

N

M

D

D

A

A

F

F

G

A

T

T

A

A

H

H

R

R

A

A

H

Q

C

A

S

S

T

T

V

Y

G

G

V

V

T

A

K

K

F

Y

V

I

D

P

T

V

A

A

V

C

C

A

G

G

Y

I

L

L

I

L

Q

T

K

N

E

E

L

I

K

Q

F

A

L

L

G

E

S

K

A

A

V

L

E

K

N

S

P

P

V

K

R

K

P

P

F

M

V

A

A

A

I

I

L

V

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

D

T

K
|
K

I

L

N

S

V

V

I

L

N

N

L

L

E

D

K

K

V

V

D

E

T

I

L

L

I

I

I

V

G

G

G
|
G

G

G

M

I

A

A

Y

N

T

T

F

F

L

I

K

K

A

A

Q

E

G

G

Y

F

T

D

I

V

G

G

T

N

S

S

L

L

L

Y

E

E

A

Q

D

D

K

L

V

V

D

A

Y

E

A

A

K

T

E

E

M

I

I

L

E

A

K

K

A

A

E

K

A

A

K

L

G

G

V

V

K

N

L

I

L

P

L

V

P

P

I

V

D

D

N

V

I

R

V

V

G

A

A

K

E

E

F

F

K

S

A

E

D

N

T

A

K

Q

A

A

V

I

T

I

T

K

E

K

D

V

A

S

N

D

I

V

P

V

E

A

G

D

Y

E

M

M

G

I

L

L

D

D

I

I

G

G

P

P

K

E

T

S

Q

Q

K

K

L

I

Y

I

A

A

D

E

A

L

V

L

K

K

E

S

A

A

K

N

T

T

V

I

V

L

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

F

E

D

N

N

F

F

A

A

K

E

G

G

T

T

K

K

D

A

V

L

A

S

K

L

A

A

M

I

A

A

E

Q

S

S

D

H

A

A

T

F

T

S

V

V

I

A

G

G

G
|
G

D
|
D

S

T

A

I

A

A

V

I

N

E

Q

K

L

F

G

G

F

I

G

K

D

D

K

Q

M

V

T

S

H

Y

I

I

S

S

T

T

G

A

G

G

G
|
G

A

A

S

F

L

L

E

E

F

F

L

L

E

E

G

G

K

K

E

K

L

L

P

P

G

A

I

I

V

E

A

I

L

L

N

K

D

E

K

K

active site: R36 (= R38), K193 (= K201), G346 (= G354), G369 (= G377)
binding adenosine-5'-diphosphate: G191 (= G199), S192 (≠ A200), K197 (= K205), G215 (= G223), G316 (= G324), V317 (= V325), E319 (= E327), D347 (= D355)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
50% identity, 99% coverage: 4:396/397 of query aligns to 3:404/405 of 2wzcA

query
sites
2wzcA

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

G

D

E

-

P

-

A

-

K

-

I

D

E

A

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (= A200), K204 (= K201), K208 (= K205), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (≠ S356)
binding tetrafluoroaluminate ion: R37 (= R38), K204 (= K201), K208 (= K205), G361 (= G353), G362 (= G354), G384 (= G376)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
50% identity, 99% coverage: 4:396/397 of query aligns to 3:404/405 of 2wzbA

query
sites
2wzbA

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

G

D

E

-

P

-

A

-

K

-

I

D

E

A

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (= A200), K204 (= K201), K208 (= K205), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (≠ S356)
binding trifluoromagnesate: K204 (= K201), K208 (= K205), G361 (= G353), G384 (= G376), G385 (= G377)

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
51% identity, 99% coverage: 5:397/397 of query aligns to 6:414/414 of O60101

query
sites
O60101

K

K

K

L

T

A

I

I

E

T

D

D

V

V

D

D

V

L

K

K

G

G

K

K

R

N

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

K

D

D

G

G

D

V

R

I

I

T

T

D

N

E

N

N

A

R

R

L

I

N

V

G

G

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

A

S

L

E

E

H

Q

G

Q

G

P

K

K

-

A

V

V

I

I

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

N

G

G

P

A

D

R

P

V

S

A

K

K

-
x
Y

T
x
S

L

L

A

K

P

P

V

V

A

A

K

A

R

E

L

L

S

S

E

K

L

L

G

G

R

K

E

P

V

V

K

K

F

F

A

L

A

-

D

-

D

D

T

D

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

K

E

E

L

A

K

K

E

G

G

G

E

E

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

P

I

E

E

-

E

-

G

-

S

G

A

K

K

N

V

D

D

-

G

-

K

-

V

-

K

-

A

-

D

-

A

-
x
S

-

A

-

V

D

E

A

A

F

F

S

R

K

K

D

S

L

L

A

T

S

S

L

L

A

G

D

D

V

I

Y

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C
x
S

S
|
S

T

M

V

V

G

G

V

V

T

D

K

L

F

-

V

-

D

-

T

P

A

R

V

V

C
x
S

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

D

F

Y

L

F

G

S

S

K

A

A

V

L

E

E

N

N

P

P

V

A

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

A

A

K

K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

E

D

K

K

V

V

D

N

T

R

L

L

I

I

G

C

G

G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

G

Y

M

T

K

I

I

G

G

T

D

S
|
S

L

L

L

F

E

D

A

E

D

A

K

G

V
x
S

D

K

Y

N

A

V

K

E

E

S

M

M

I

M

E

A

K

K

A

A

E

K

A

K

K

N

G

N

V

V

K

E

L

V

L

F

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

K

D

D

T

A

K

K

-

V

A

G

V

S

T

A

T
|
T

E

A

D

E

A

E

N

G

I

I

P

P

E

D

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

K

T

S

Q

S

K

A

L

K

Y

F

A

A

D

E

A

V

V

I

K

T

E

T

A
x
S

K

K

T

T

V

I

V

V

W

W

N

N

G

G

P

P

M

A

G

G

V

V

F

F

E

E

F

F

E

D

N

N

F

F

A

A

K

K

G

G

T

T

K

K

D
x
S

V

M

A

L

K

D

A

A

M

C

-

V

-

K

-

T

A

C

E

E

S

A

D

G

A

N

T

V

V

I

I

V

G

G

D

D

S
x
T

A

A

A

T

A

V

V

A

N

K

Q

K

L

Y

G

G

F

K

G

E

D

D

K

A

M

L

T
x
S

H

H

I

V

S
|
S

T

T

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

A

L

L

P

P

G

G

I

V

V

V

A

A

L

L

N
x
S

D
x
S

K

K

Y75 (vs. gap) modified: Phosphotyrosine
S76 (≠ T73) modified: Phosphoserine
S143 (vs. gap) modified: Phosphoserine
S172 (≠ C157) modified: Phosphoserine
S173 (= S158) modified: Phosphoserine
S183 (≠ C171) modified: Phosphoserine
S253 (= S240) modified: Phosphoserine
S260 (≠ V247) modified: Phosphoserine
T299 (= T285) modified: Phosphothreonine
S328 (≠ A314) modified: Phosphoserine
S351 (≠ D337) modified: Phosphoserine
T373 (≠ S356) modified: Phosphothreonine
S387 (≠ T370) modified: Phosphoserine
S390 (= S373) modified: Phosphoserine
S412 (≠ N395) modified: Phosphoserine
S413 (≠ D396) modified: Phosphoserine

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
49% identity, 99% coverage: 4:397/397 of query aligns to 4:414/415 of 3pgkA

query
sites
3pgkA

N

S

K

K

K

L

T

S

I

V

E

Q

D

D

V

L

D

D

V

L

K

K

G

D

K

K

R

R

V

V

L

F

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

K

D

D

G

G

K

V

K

I

I

T

T

D

S

E

N

N

Q

R
|
R

L

I

N

V

G

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

V

S

L

E

E

H

H

G

H

G

P

K

R

-

Y

V

V

I

V

L

L

C

A

S

S

H

H

L

L

G

G

K

R

A

P

K

N

G

G

P

E

D

R

P

N

S

E

K

K

-

Y

T

S

L

L

A

A

P

P

V

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

G

G

R

K

E

D

V

V

K

T

F

F

A

L

A

N

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

A

A

A

V

V

A

K

E

A

L

S

K

A

E

P

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

Y

R

H

P

I

E

E

-

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

G

S

K

K

N

E

D

D

-

V

D

Q

A

K

F

F

S

R

K

H

D

E

L

L

A

S

S

S

L

L

A

A

D

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

F

T

D

K

-

F

-

V

L

D

P

T

Q

A

R

V

A

C

A

G

G

Y

F

L

L

I

L

Q

E

K

K

E

E

L

L

K

K

F

Y

L

F

G

G

S

K

A

A

V

L

E

E

N

N

P

P

V

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

E

D

K

K

V

V

D

D

T

S

L

I

I

I

G

G

M

M

A

A

Y

F

T

T

F

F

L

K

K

K

A

V

-

L

Q

E

G

N

Y

T

T

E

I

I

G

G

T

D

S

S

L

I

L

F

E

D

A

K

D

A

K

V

V

G

D

P

Y

E

A

I

K

A

E

K

M

L

I

M

E

E

K

K

A

A

E

K

A

A

K

K

G

G

V

V

K

E

L

V

L

L

P

P

I

V

D

D

N

F

I

I

V

I

G

A

A

D

E

A

F
|
F

-

S

-

A

K

S

A

A

D

N

T

T

K

K

A

T

V

V

T

T

T

D

E

K

D

E

A

G

N

-

I

I

P

P

E

A

G

G

Y

W

M

Q

G

G

L
|
L

D

D

I

N

G

G

P

P

K

E

T

S

Q

R

K

K

L

L

Y

F

A

A

D

A

A

T

V

V

K

A

E

K

A

A

K

T

T

V

V

I

V

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

F

F

E

E

F

F

E

E

N

K

F

F

A

A

K

A

G

G

T

T

K

K

D

A

V

L

A

L

K

D

A

E

M

V

A

V

E

K

S

S

D

S

A

A

-

A

-

G

-

N

T

T

T

V

V

I

I

I

G

G

G
|
G

D
|
D

S

T

A

A

A

T

A

V

V

A

N

K

Q

K

L

Y

G

G

F

V

G

T

D

D

K

K

M

I

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

V

A

A

F

L

L

N

S

D

E

K

K

active site: R38 (= R38), K213 (= K201), G371 (= G354), G394 (= G377)
binding adenosine-5'-triphosphate: G211 (= G199), A212 (= A200), K213 (= K201), F289 (= F276), L311 (= L297), N334 (= N320), G335 (= G321), P336 (= P322), G338 (= G324), V339 (= V325), D372 (= D355)

2wzdA The catalytically active fully closed conformation of human phosphoglycerate kinase k219a mutant in complex with adp, 3pg and aluminium trifluoride (see paper)
50% identity, 99% coverage: 4:396/397 of query aligns to 3:404/405 of 2wzdA

query
sites
2wzdA

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

G

D

E

-

P

-

A

-

K

-

I

D

E

A

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K

A

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (= A200), K204 (= K201), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (≠ S356)
binding aluminum fluoride: R37 (= R38), K204 (= K201), G361 (= G353), G362 (= G354), G384 (= G376)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
50% identity, 99% coverage: 4:396/397 of query aligns to 3:406/407 of 4axxA

query
sites
4axxA

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

A

D

K

-

A

-

E

-

P

-

A

-

K

-

I

D

E

A

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K206 (= K201), G364 (= G354), G387 (= G377)
binding adenosine-5'-diphosphate: G204 (= G199), A205 (= A200), K210 (= K205), G228 (= G223), G229 (= G224), N327 (= N320), P329 (= P322), G331 (= G324), V332 (= V325), E334 (= E327), G363 (= G353), G364 (= G354), D365 (= D355), T366 (≠ S356)
binding beryllium trifluoride ion: K206 (= K201), K210 (= K205), G363 (= G353)

2x15A The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp and 1,3- bisphosphoglycerate
50% identity, 99% coverage: 4:396/397 of query aligns to 3:407/408 of 2x15A

query
sites
2x15A

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D
|
D

F

F

N
|
N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H
|
H

L

L

G

G

K
x
R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R
|
R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

G

D

K

-

K

-

A

-

E

-

P

-

A

-

K

-

I

D

E

A

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F

F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K207 (= K201), G365 (= G354), G388 (= G377)
binding adenosine-5'-diphosphate: G205 (= G199), A206 (= A200), K207 (= K201), K211 (= K205), G229 (= G223), G230 (= G224), N328 (= N320), P330 (= P322), G332 (= G324), V333 (= V325), E335 (= E327), G364 (= G353), G365 (= G354), D366 (= D355), T367 (≠ S356)
binding adenosine-5'-triphosphate: G205 (= G199), A206 (= A200), K207 (= K201), K211 (= K205), G229 (= G223), G230 (= G224), N328 (= N320), G332 (= G324), V333 (= V325), E335 (= E327), G364 (= G353), G365 (= G354), D366 (= D355), T367 (≠ S356), G387 (= G376), G388 (= G377)
binding 1,3-bisphosphoglyceric acid: D22 (= D23), N24 (= N25), R37 (= R38), H61 (= H61), R64 (≠ K64), R121 (= R121), R162 (= R154), K207 (= K201), K211 (= K205), G364 (= G353), G387 (= G376), G388 (= G377)

2y3iA The structure of the fully closed conformation of human pgk in complex with l-adp, 3pg and the tsa aluminium tetrafluoride (see paper)
49% identity, 99% coverage: 4:396/397 of query aligns to 3:414/414 of 2y3iA

query
sites
2y3iA

N

N

K

K

K

L

T

T

I

L

E

D

D

K

V

L

D

D

V

V

K

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

K

K

D

N

G

N

V

Q

I

I

T

T

D

N

E

N

N

Q

R
|
R

L

I

N

K

G

A

A

A

L

V

P

P

T

S

I

I

K

K

Y

F

L

C

S

L

E

D

H

N

G

G

G

A

K

K

-

S

V

V

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

A

P

K

D

G

G

-

V

P

P

D

M

P

P

S

D

K

K

-

Y

T

S

L

L

A

E

P

P

V

V

A

A

K

V

R

E

L

L

S

K

E

S

L

L

G

G

R

K

E

D

V

V

K

L

F

F

A

L

A

K

D

D

D

-

T

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

K

K

A

A

V

C

A

A

E

N

L

P

K

A

E

A

G

G

E

S

I

V

V

I

L

L

E

E

N

N

T

L

R

R

F

F

R

H

-

V

P

E

E

E

G

G

K

K

N

G

D

K

D

D

A

A

-

S

-

G

-

N

-

K

-

V

-

K

-

A

-

E

-

P

-

A

-

K

-

I

-

E

-

A

F

F

S

R

K

A

D

S

L

L

A

S

S

K

L

L

A

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

V

T

N

K

-

F

-

V

L

D

P

T

Q

A

K

V

A

C

G

G

G

Y

F

L

L

I

M

Q

K

K

K

E

E

L

L

K

N

F

Y

L

F

G

A

S

K

A

A

V

L

E

E

N

S

P

P

V

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

N

L

M

L

L

E

D

K

K

V

V

D

N

T

E

L

M

I

I

G

G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

Y

M

T

E

I

I

G

G

T

T

S

S

L

L

L

F

E

D

A

E

D

E

K

G

V

A

D

K

Y

I

A

V

K

K

E

D

M

L

I

M

E

S

K

K

A

A

E

E

A

K

K

N

G

G

V

V

K

K

L

I

L

T

L

L

P

P

I

V

D

D

N

F

I

V

V

T

G

A

A

D

E

K

F
|
F

K

D

A

E

D

N

T

A

K

K

A

T

-

G

V

Q

T

A

T

T

E

V

D

A

A

S

N

G

I

I

P

P

E

A

G

G

Y

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

K

E

T

S

Q

S

K

K

L

K

Y

Y

A

A

D

E

A

A

V

V

K

T

E

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

E

E

N

A

F

F

A

A

K

R

G

G

T

T

K

K

-

A

-

L

-

M

-

D

D

E

V

V

A

V

K

K

A

A

M

T

A

S

E

R

S

G

D

-

A

C

T

I

T

T

V

I

I

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

N

A

Q

K

L

W

G

N

F

T

G

E

D

D

K

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

V

D

A

A

L

L

N

S

D

N

active site: R37 (= R38), K214 (= K201), G372 (= G354), G395 (= G377)
binding tetrafluoroaluminate ion: K214 (= K201), G371 (= G353), G372 (= G354), G394 (= G376)
binding l-adenosine-5'-diphosphate: G212 (= G199), A213 (= A200), F290 (= F276), N335 (= N320), G339 (= G324), V340 (= V325), E342 (= E327), G371 (= G353), G372 (= G354), D373 (= D355), T374 (≠ S356)

Query Sequence

>WP_010964029.1 NCBI__GCF_000008765.1:WP_010964029.1
MKFNKKTIEDVDVKGKRVLVRCDFNVPLKDGVITDENRLNGALPTIKYLSEHGGKVILCS
HLGKAKGPDPSKTLAPVAKRLSELLGREVKFAADDTVVGENAKKAVAELKEGEIVLLENT
RFRPEEGKNDDAFSKDLASLADVYVNDAFGTAHRAHCSTVGVTKFVDTAVCGYLIQKELK
FLGSAVENPVRPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTIGTS
LLEADKVDYAKEMIEKAEAKGVKLLLPIDNIVGAEFKADTKAVTTEDANIPEGYMGLDIG
PKTQKLYADAVKEAKTVVWNGPMGVFEFENFAKGTKDVAKAMAESDATTVIGGGDSAAAV
NQLGFGDKMTHISTGGGASLEFLEGKELPGIVALNDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory