SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964137.1 NCBI__GCF_000008765.1:WP_010964137.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
25% identity, 93% coverage: 12:357/371 of query aligns to 3:314/315 of P0A717

query
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P0A717

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D129 (= D156) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D260) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D261) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ A264) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
26% identity, 93% coverage: 12:355/371 of query aligns to 1:310/311 of 6asvC

query
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6asvC

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binding phosphate ion: D168 (= D204), D218 (= D260), D219 (= D261), D222 (≠ A264), T223 (≠ S265), G224 (= G266), T226 (≠ S268), L227 (≠ V269)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
26% identity, 92% coverage: 12:352/371 of query aligns to 2:308/308 of 4s2uA

query
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4s2uA

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binding magnesium ion: D223 (≠ A264), T227 (≠ S268)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
25% identity, 93% coverage: 12:357/371 of query aligns to 1:306/307 of 7xmvA

query
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7xmvA

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P

I

I

M

L

E

L

E

E

K

D

N

M

I

L

E

Q

V

L

N

N

K

L

N

D

S

N

M

P

L

I

V

V

I

V

S

S

P

P

D

D

T

I

G

G

A

G

M

V

D
x
V

R

R

A

A

I

R

Y
x
A

Y

I

S

A

S

K

V

L

L

L

G

N

-

D

V

T

D

D

V

M

G

A

L

I

F

I

Y

D

K

K

R

R

D

Q

H

V

S

M

T

H

I

I

V

I

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

D

V

V

E

A

N

G

Q

R

D

D

V

C

L

V

I

L

V

V

D
|
D

M
|
M

I

I

A
x
D

S
x
T

G

G

E
x
G

S
x
T

V

L

L

C

D

K

I

A

A

A

K

E

E

A

L

L

K

K

A

E

R

R

K

G

A

A

R

K

N

R

I

V

Y

F

V

A

A

Y

T

A

T

T

F

H

A

P

F

I

F

F

T

S

E

G

G

N

L

A

E

A

K

N

F

N

Q

L

K

R

Y

-

D

-

D

N

N

S

I

V

I

I

S

D

R

E

V

V

Y

V

S

V

T

C

N

D

L

T

T

I

Y

P

I

L

P

S

P

D

E

E

L

I

E

K

K

S

T

L

E

P

W

N

F

V

R

R

K

T

V

L

D

T

M

L

S

S

E

G

L

M

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

I

N

S

K

N

D

E

E

E

S

S

I

I

A

S

K

A

F

M

M

F

D

E

binding adenosine-5'-diphosphate: F33 (= F51), D35 (≠ N53), E37 (= E55), R94 (= R125), R97 (= R128), H129 (= H158)
binding adenosine monophosphate: R97 (= R128), V99 (vs. gap), R100 (vs. gap), E131 (≠ P160), F145 (≠ V181), S147 (≠ T183), V173 (≠ D209), A177 (≠ Y213)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D260), D213 (= D261), M214 (= M262), D216 (≠ A264), T217 (≠ S265), G219 (≠ E267), T220 (≠ S268)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
25% identity, 93% coverage: 12:357/371 of query aligns to 1:306/307 of 7xmuA

query
sites
7xmuA

E

D

L

M

G

K

I

L

V

F

A

A

L

G

E

N

S

A

C

T

R

P

E

E

L

L

G

A

Q

Q

K

R

I

I

D

A

N

N

R

R

I

L

K

Y

T

T

K

S

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

D

T

A

Q

A

E

V

I

G

R

R

F
|
F

S

S

N
x
D

G

G

E
|
E

G

V

K

S

V

V

K

Q

L

I

S

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

I

I

Y

F

I

I

L

I

C

-

D

-

V

-

G

-

N

Q

Y

S

S

T

C

C

T

A

Y

P

N

T

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

-

P

-

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

V

R

V

T

M

V

V

S

D

A

A

I

L

R

R

-

R

G

A

K

S

A

A

R

G

R

R

I

I

T

T

V

A

I

V

M

I

P

P

L

Y

L

F

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R
|
R

R

R

-

V

-
x
R

K

S

G

A

R

R

E

V

S

P

L

I

D

T

C

A

A

K

L

V

A

V

L

A

Q

D

E

F

L

L

E

S

R

S

L

V

G

G

V

V

Q

D

E

R

V

V

L

L

T

T

F

V

D

D

A

L

H
|
H

D

A

P
x
E

N

Q

V

I

Q

Q

N

G

A

F

I

F

P

D

L

V

M

-

S

-

F

-

E

-

N

-

L

-

Y

-

P

P

T

V

Y

D

D

N

I

V

V
x
F

K

G

T
x
S

I

P

I

I

M

L

E

L

E

E

K

D

N

M

I

L

E

Q

V

L

N

N

K

L

N

D

S

N

M

P

L

I

V

V

I

V

S

S

P

P

D
|
D

T

I

G

G

A

G

M

V

D
x
V

R

R

A

A

I

R

Y

A

Y

I

S

A

S

K

V

L

L

L

G

N

-

D

V

T

D

D

V

M

G

A

L

I

F

I

Y

D

K

K

R

R

D

Q

H

V

S

M

T

H

I

I

V

I

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

D

V

V

E

A

N

G

Q

R

D

D

V

C

L

V

I

L

V

V

D
|
D

D

D

M
|
M

I

I

A
x
D

S
x
T

G

G

E

G

S

T

V

L

L

C

D

K

I

A

A

A

K

E

E

A

L

L

K

K

A

E

R

R

K

G

A

A

R

K

N

R

I

V

Y

F

V

A

A

Y

T

A

T

T

F

H

A

P

F

I

F

F

T

S

E

G

G

N

L

A

E

A

K

N

F

N

Q

L

K

R

Y

-

D

-

D

N

N

S

I

V

I

I

S

D

R

E

V

V

Y

V

S

V

T

C

N

D

L

T

T

I

Y

P

I

L

P

S

P

D

E

E

L

I

E

K

K

S

T

L

E

P

W

N

F

V

R

R

K

T

V

L

D

T

M

L

S

S

E

G

L

M

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

I

N

S

K

N

D

E

E

E

S

S

I

I

A

S

K

A

F

M

M

F

D

E

binding adenosine-5'-diphosphate: F33 (= F51), D35 (≠ N53), E37 (= E55), R94 (= R125), Q95 (= Q126), R97 (= R128), R97 (= R128), R100 (vs. gap), H129 (= H158), E131 (≠ P160), F145 (≠ V181), S147 (≠ T183), V173 (≠ D209)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D204), D212 (= D260), M214 (= M262), D216 (≠ A264), T217 (≠ S265)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 91% coverage: 15:353/371 of query aligns to 8:313/321 of O94413

query
sites
O94413

I

I

V

F

A

A

L

G

E

N

S

S

C

H

R

P

E

E

L

L

G

A

Q

E

K

K

I

V

D

A

N

R

R

R

I

I

K

G

T

L

K

S

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

A

E

V

I

V

R

Q

F

Y

S

S

N

N

G

R

E

E

G

T

K

S

V

V

K

T

L

I

S

G

E

E

S

S

V

V

R

R

G

D

K

E

D

D

I

V

Y

F

I

I

L

L

C

-

D

-

V

-

G

-

N

Q

Y

T

S

G

C

C

T

-

Y

-

N

-

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

G

G

S

P

I

D

N

E

D

H

H

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

R

-

S

G

A

K

S

A

A

R

R

I

I

T

T

V

A

I

I

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R

R

Q

Q

H

D

R

K

-

D

K

K

G

S

R

R

E

A

S

P

L

I

D

T

C

A

A

R

L

L

A

V

L

A

Q

N

E

M

L

L

E

Q

R

T

L

A

G

G

V

C

Q

N

E

H

V

I

L

I

T

T

F

M

D

D

A

L

H

H

D

A

P

S

N

Q

V

I

Q

Q

N

G

-

F

A

N

I

V

P

P

L

V

M

-

S

-

F

-

E

D

N

N

L

L

Y

Y

P

A

T

E

Y

P

D

S

I

V

V

L

K

R

T

Y

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

D

V

T

N

T

K

V

N

N

S

P

M

T

L

V

V

I

I

V

S
|
S

P

P

D

D

T

A

G

G

A

G

M

A

D

K

R

R

A

A

I

T

Y

A

Y

L

S

A

S

D

V

R

L

L

G

D

V

L

D

D

V

F

G

A

L

L

F

I

Y

H

K

K

R

E

R

R

D

Q

H

K

S

A

T

-

I

-

V

-

N

-

G

-

K

-

N

N

P

E

I

V

V

S

K

R

H

M

E

V

Y

L

M

V

G

G

R

-

D

D

V

V

E

R

N

D

Q

K

D

L

V

A

L

I

I

L

V

V

D

D

M

M

I

A

A

D

S

T

G

C

E

G

S

T

V

L

L

G

D

L

I

A

A

A

K

K

E

T

L

L

K

K

A

D

R

N

K

G

A

A

R

K

N

A

I

V

Y

Y

V

A

A

I

T

V

T

T

F

H

A

G

F

I

F

L

T

S

E

-

G

-

L

-

E

G

K

K

F

A

Q

I

K

K

Y

V

Y

I

D

N

D

E

N

S

I

A

I

L

S

E

R

K

V

V

Y

I

S

V

T

T

N

N

L

-

T

-

Y

T

I

I

P

P

P

H

E

D

L

D

E

K

K

R

T

S

-

L

-

C

E

S

W

K

F

I

R

E

K

T

V

I

D

D

M

I

S

S

E

G

L

V

I

L

S

A

R

E

I

C

I

I

H

R

K

R

L

I

N

H

K

H

D

G

E

E

S

S

I

V

A

S

S172 (= S202) modified: Phosphoserine

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
28% identity, 67% coverage: 50:296/371 of query aligns to 37:258/318 of Q63XL8

query
sites
Q63XL8

R

R

F

F

S

S

N

D

G

G

E

E

G

I

K

Q

V

V

K

E

L

I

S

Q

E

E

S

N

V

V

R

R

G

G

K

K

D

D

I

V

Y

F

I

V

L

L

C

-

D

-

V

-

G

-

N

Q

Y

S

S

T

C

C

T

A

Y

P

N

T

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

-

P

-

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

M

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

G

R

R

I

I

T

T

V

A

I

A

M

I

P

P

L

Y

L

F

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

R

-

R

-

P

R

R

K

S

G

A

R

R

E

V

S

A

L

I

D

S

C

A

A

K

L

V

A

V

L

A

Q

N

E

M

L

L

E

E

R

I

L

A

G

G

V

V

Q

E

E

R

V

I

L

I

T

T

F

M

D

D

A

L

H

H

D

A

P

D

N

Q

V

I

Q

Q

N

G

A

-

I

-

P

-

L

-

M

-

S

-

F

F

E

F

N

D

L

I

Y

-

P

P

T

V

Y

D

D

N

I

I

V

Y

K

A

T

T

I

P

I

I

M

L

E

L

E

G

K

D

N

L

I

R

E

K

V

Q

N

N

K

Y

N

P

S

D

M

L

L

L

V

V

I

V

S

S

P

P

D

D

T

V

G

G

A

G

M

V

D

V

R

R

A

A

I

R

Y

A

Y

L

S

A

S

K

V

Q

L

L

G

N

V

C

D

D

V

L

G

A

L

I

F

I

Y

D

K

K

R

R

D

P

H

K

S

A

T

N

I

V

V

A

N

E

G

V

K

M

N

N

P

I

I

I

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

G

D

E

V

V

E

E

N

G

Q

R

D

T

V

C

L

V

I

I

V

M

D

D

M

M

I

V

A

D

S

T

G

A

E

G

S

T

V

L

L

C

D

K

I

A

A

A

K

Q

E

V

L

L

K

K

A

E

R

R

K

G

A

A

R

K

N

Q

I

V

Y

F

V

A

A

Y

T

A

T

T

F

H

A

P

F

V

F

L

T

S

E

G

G

G

RQ 99:100 (= RQ 125:126) binding ATP

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
24% identity, 93% coverage: 8:353/371 of query aligns to 5:313/317 of P14193

query
sites
P14193

Y

Y

R

G

N

D

G

K

E

N

L

L

G

K

I

I

V

F

A

S

L

L

E

N

S

S

C

N

R

P

E

E

L

L

G

A

Q

K

K

E

I

I

D

A

N

D

R

I

I

V

K

G

T

V

K

Q

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

C

Q

S

E

V

I

T

R

R

F

F

S

S

N

D

G

G

E

E

G

V

K

Q

V

I

K

N

L

I

S

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

I

C

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

I

E

Q

N

S

H

T

K

S

G

D

P

P

-

V

D

N

E

E

H

H

F

I

Q

M

D

E

I

L

K

L

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

K

R

T

I

I

T

N

V

I

I

V

M

I

P

P

L

Y

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

R

R

K

-

A

K

R

G

S

R

R

E

E

S

P

L

I

D

T

C

A

A

K

L

L

A

F

L

A

Q

N

E

L

L

L

E

E

R

T

L

A

G

G

V

A

Q

T

E

R

V

V

L

I

T

A

F

L

D

D

A

L

H

H

D

A

P

P

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

-

I

-

D

-

H

L

L

M

M

S

G

F

V

E

P

N

I

L

L

Y

G

P

E

T

Y

Y

F

D

-

I

-

V

-

K

-

T

-

I

-

M

-

E

E

E

G

K

K

N

N

I

L

E

E

V

-

N

-

K

-

N

-

S

D

M

I

L

V

V

I

I

V

S

S

P

P

D

D

T

H

G

G

A

G

M

V

D

T

R

R

A

A

I

R

Y

K

Y

L

S

A

S

D

V

R

L

L

G

K

V

A

D

P

V

I

G

A

L

I

F

I

Y

D

K
|
K

R

R

R
|
R

D

P

H
x
R

S

P

T
x
N

I

V

V

A

N
x
E

G

V

K

M

N

N

P

I

I

V

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

G

D

N

V

I

E

E

N

G

Q

K

D

T

V

A

L

I

I

L

V

I

D

D

M

I

I

I

A
x
D

S
x
T

G
x
A

E
x
G

S
x
T

V

I

L

T

D

L

I

A

A

A

K

N

E

A

L

L

K

V

A

E

R

N

K

G

A

A

R

K

N

E

I

V

Y

Y

V

A

A

C

T

C

T

T

F

H

A

P

F

V

F

L

T

S

-

G

E

P

G

A

L

V

E

E

K

R

F

I

Q

-

K

-

Y

-

D

N

N

S

I

T

I

I

S

K

R

E

V

L

Y

V

S

V

T

T

N

N

L

S

T

I

Y

K

I

L

P

P

P

E

E

E

L

-

E

K

K

K

T

I

E

E

W

R

F

F

R

K

K

Q

V

L

D

S

M

V

S

G

E

P

L

L

I

L

S

A

R

E

I

A

I

I

H

I

K

R

L

V

N

H

K

E

D

Q

E

Q

S

S

I

V

A

S

RQ 102:103 (= RQ 125:126) binding ATP
K198 (= K227) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R229) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ H231) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ T233) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ N236) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ ASGES 264:268) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
27% identity, 67% coverage: 50:296/371 of query aligns to 32:246/300 of 3dahC

query
sites
3dahC

R

R

F
|
F

S

S

N
x
D

G

G

E
|
E

G

I

K

Q

V

V

K

E

L

I

S

Q

E

E

S

N

V

V

R

R

G

G

K

K

D

D

I

V

Y

F

I

V

L

L

C

-

D

-

V

-

G

-

N

Q

Y

S

S

T

C

C

T

A

Y

P

N

T

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

-

P

-

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

M

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

G

R

R

I

I

T

T

V

A

I

A

M

I

P

P

L

Y

L

F

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

R

-

R

-

P

R

R

K

S

G

A

R

R

E

V

S

A

L

I

D

S

C

A

A

K

L

V

A

V

L

A

Q

N

E

M

L

L

E

E

R

I

L

A

G

G

V

V

Q

E

E

R

V

I

L

I

T

T

F

M

D

D

A

L

H

H

D

A

P

D

N

Q

V

I

Q

Q

N

G

A

-

I

-

P

-

L

-

M

-

S

-

F

F

E

F

N

D

L

I

Y

-

P

P

T

V

Y

D

D

N

I

I

V

Y

K

A

T

T

I

P

I

I

M

L

E

L

E

G

K

D

N

L

I

R

E

K

V

Q

N

N

K

Y

N

P

S

D

M

L

L

L

V

V

I

V

S

S

P

P

D

D

T

V

G

G

A

G

M

V

D

V

R

R

A

A

I

R

Y

A

Y

L

S

A

S

K

V

Q

L

L

G

N

V

C

D

D

V

L

G

A

L

I

F

I

Y

D

K

K

R

R

D

V

H

M

S

N

T

I

I

I

V

-

N

-

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

G

D

E

V

V

E

E

N

G

Q

R

D

T

V

C

L

V

I

I

V

M

D

D

M

M

I

V

A

D

S

T

G

A

E

G

S

T

V

L

L

C

D

K

I

A

A

A

K

Q

E

V

L

L

K

K

A

E

R

R

K

G

A

A

R

K

N

Q

I

V

Y

F

V

A

A

Y

T

A

T

T

F

H

A

P

F

V

F

L

T

S

E

G

G

G

binding adenosine monophosphate: F33 (= F51), D35 (≠ N53), E37 (= E55), R94 (= R125), Q95 (= Q126)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
24% identity, 89% coverage: 22:353/371 of query aligns to 11:302/305 of 2hcrA

query
sites
2hcrA

R

Q

E

D

L

L

G

S

Q

Q

K

K

I

I

D

A

N

D

R

R

I

L

K

G

T

L

K

E

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

V

E

T

I

K

R

K

F

F

S

S

N

N

G

Q

E

E

G

T

K

C

V

V

K

E

L

I

S

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

I

V

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

V

E

Q

N

S

H

G

K

C

G

G

P

E

-

I

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

K

-

I

G

A

K

S

A

A

R

S

R

R

I

V

T

T

V

A

I

V

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R
|
R

Q
|
Q

H

D

R
x
K

R

K

-

D

K

K

G

S

R

R

E

A

S

P

L

I

D

S

C

A

A

K

L

L

A

V

L

A

Q

N

E

M

L

L

E

S

R

V

L

A

G

G

V

A

Q

D

E

H

V

I

L

I

T

T

F

M

D

D

A

L

H
|
H

D

A

P

S

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

L

V

M

-

S

-

F

-

E

D

N

N

L

L

Y

Y

P

A

T

E

Y

P

D

A

I

V

V

L

K

K

T

W

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

S

V

E

N

W

K

R

N

N

S

C

M

T

L

I

V

V

I

-

S

S

P

P

D

D

T

A

G

G

A

G

M

A

D

K

R

R

A

V

I

T

Y

S

Y

I

S

A

S

D

V

R

L

L

G

N

V

V

D

D

V

F

G

A

L

L

F

I

Y

H

K

K

R

E

R

R

D

D

H

R

S

M

T

V

I

L

V

V

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

D

V

V

E

K

N

D

Q

R

D

V

V

A

L

I

I

L

V

V

D

D

M

M

I

A

A

D

S

T

G

C

E

G

S

T

V

I

L

C

D

H

I

A

A

A

K

D

E

K

L

L

K

L

A

S

R

A

K

G

A

A

R

T

N

R

I

V

Y

Y

V

A

A

I

T

L

T

T

F

H

A

G

F

I

F

F

T

S

-

G

E

P

G

A

L

I

E

S

K

R

F

I

Q

-

K

-

Y

-

D

N

N

A

I

C

I

F

S

E

R

A

V

V

Y

V

S

V

T

T

N

N

L

-

T

-

Y

T

I

I

P

P

P

Q

E

E

-

D

-

K

L

M

E

K

K

H

T

C

E

S

W

K

F

I

R

Q

K

V

V

I

D

D

M

I

S

S

E

M

L

I

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

T

N

H

K

N

D

G

E

E

S

S

I

V

A

S

binding adenosine monophosphate: R94 (= R125), Q95 (= Q126), K97 (≠ R128), H128 (= H158)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
25% identity, 82% coverage: 50:353/371 of query aligns to 32:294/297 of 1ibsA

query
sites
1ibsA

R

R

F

F

S

S

N

D

G

G

E

E

G

V

K

Q

V

I

K

N

L

I

S

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

I

C

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

I

E

Q

N

S

H

T

K

S

G

D

P

P

-

V

D

N

E

E

H

H

F

I

Q

M

D

E

I

L

K

L

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

K

R

T

I

I

T

N

V

I

I

V

M

I

P

P

L

Y

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

-

R
|
R

K

K

G

S

R

R

E

E

S

P

L

I

D

T

C

A

A

K

L

L

A

F

L

A

Q

N

E

L

L

L

E

E

R

T

L

A

G

G

V

A

Q

T

E

R

V

V

L

I

T

A

F

L

D

D

A

L

H
|
H

D

A

P

P

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

-

I

-

D

-

H

L

L

M

M

S

G

F

V

E

P

N

I

L

L

Y

G

P

E

T

Y

Y

F

D

-

I

-

V

-

K

-

T

-

I

-

M

-

E

E

E

G

K

K

N

N

I

L

E

E

V

-

N

-

K

-

N

-

S

D

M

I

L

V

V

I

I

V

S

S

P

P

D

D

T

H

G

G

A

G

M

V

D

T

R

R

A

A

I

R

Y

K

Y

L

S

A

S

D

V

R

L

L

G

K

V

A

D

P

V

I

G

A

L

I

F

I

Y

D

K

K

R

R

R

M

D

N

H

-

S

-

T

-

I

I

V

V

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

N

V

I

E

E

N

G

Q

K

D

T

V

A

L

I

I

L

V

I

D

D

M

I

I

I

A
x
D

S
x
T

G
x
A

E

G

S
x
T

V

I

L

T

D

L

I

A

A

A

K

N

E

A

L

L

K

V

A

E

R

N

K

G

A

A

R

K

N

E

I

V

Y

Y

V

A

A

C

T

C

T

T

F

H

A

P

F

V

F

L

T

S

-

G

E

P

G

A

L

V

E

E

K

R

F

I

Q

-

K

-

Y

-

D

N

N

S

I

T

I

I

S

K

R

E

V

L

Y

V

S

V

T

T

N

N

L

S

T

I

Y

K

I

L

P

P

P

E

E

E

L

-

E

K

K

K

T

I

E

E

W

R

F

F

R

K

K

Q

V

L

D

S

M

V

S

G

E

P

L

L

I

L

S

A

R

E

I

A

I

I

H

I

K

R

L

V

N

H

K

E

D

Q

E

Q

S

S

I

V

A

S

binding methyl phosphonic acid adenosine ester: R94 (= R125), Q95 (= Q126), R97 (= R129), H126 (= H158)
binding sulfate ion: D209 (≠ A264), T210 (≠ S265), A211 (≠ G266), T213 (≠ S268)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
25% identity, 82% coverage: 50:353/371 of query aligns to 34:296/299 of 1ibsB

query
sites
1ibsB

R

R

F

F

S

S

N

D

G

G

E

E

G

V

K

Q

V

I

K

N

L

I

S

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

I

C

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

I

E

Q

N

S

H

T

K

S

G

D

P

P

-

V

D

N

E

E

H

H

F

I

Q

M

D

E

I

L

K

L

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

K

R

T

I

I

T

N

V

I

I

V

M

I

P

P

L

Y

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

-

R
|
R

K

K

G

S

R

R

E

E

S

P

L

I

D

T

C

A

A

K

L

L

A

F

L

A

Q

N

E

L

L

L

E

E

R

T

L

A

G

G

V

A

Q

T

E

R

V

V

L

I

T

A

F

L

D

D

A

L

H

H

D

A

P

P

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

-

I

-

D

-

H

L

L

M

M

S

G

F

V

E

P

N

I

L

L

Y

G

P

E

T

Y

Y

F

D

-

I

-

V

-

K

-

T

-

I

-

M

-

E

E

E

G

K

K

N

N

I

L

E

E

V

-

N

-

K

-

N

-

S

D

M

I

L

V

V

I

I

V

S

S

P

P

D

D

T

H

G

G

A

G

M

V

D

T

R

R

A

A

I

R

Y

K

Y

L

S

A

S

D

V

R

L

L

G

K

V

A

D

P

V

I

G

A

L

I

F

I

Y

D

K

K

R

R

R

M

D

N

H

-

S

-

T

-

I

I

V

V

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

N

V

I

E

E

N

G

Q

K

D

T

V

A

L

I

I

L

V

I

D

D

M

I

I

I

A

D

S

T

G

A

E

G

S

T

V

I

L

T

D

L

I

A

A

A

K

N

E

A

L

L

K

V

A

E

R

N

K

G

A

A

R

K

N

E

I

V

Y

Y

V

A

A

C

T

C

T

T

F

H

A

P

F

V

F

L

T

S

-

G

E

P

G

A

L

V

E

E

K

R

F

I

Q

-

K

-

Y

-

D

N

N

S

I

T

I

I

S

K

R

E

V

L

Y

V

S

V

T

T

N

N

L

S

T

I

Y

K

I

L

P

P

P

E

E

E

L

-

E

K

K

K

T

I

E

E

W

R

F

F

R

K

K

Q

V

L

D

S

M

V

S

G

E

P

L

L

I

L

S

A

R

E

I

A

I

I

H

I

K

R

L

V

N

H

K

E

D

Q

E

Q

S

S

I

V

A

S

binding methyl phosphonic acid adenosine ester: R96 (= R125), Q97 (= Q126), R99 (= R129)

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
24% identity, 89% coverage: 22:353/371 of query aligns to 12:309/316 of 8dbkB

query
sites
8dbkB

R

Q

E

D

L

L

G

S

Q

Q

K

K

I

I

D

A

N

D

R

R

I

L

K

G

T

L

K

E

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

V

E

T

I

K

R

K

F
|
F

S

S

N
|
N

G

Q

E
|
E

G

T

K

C

V

V

K

E

L

I

S

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

I

V

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

V

E

Q

N

S

H

G

K

C

G

G

P

E

-

I

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

K

-

I

G

A

K

S

A

A

R

S

R

R

I

V

T

T

V

A

I

V

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

K

-

D

K

K

G

S

R

R

E

A

S

P

L

I

D

S

C

A

A

K

L

L

A

V

L

A

Q

N

E

M

L

L

E

S

R

V

L

A

G

G

V

A

Q

D

E

H

V

I

L

I

T

T

F

M

D

D

A

L

H
|
H

D

A

P

S

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

L

V

M

-

S

-

F

-

E

D

N

N

L

L

Y

Y

P

A

T

E

Y

P

D

A

I

V

V

L

K

K

T

W

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

S

V

E

N

W

K

R

N

N

S

C

M

T

L

I

V

V

I

-

S

S

P

P

D
|
D

T

A

G
|
G

A

G

M

A

D

K

R

R

A

V

I

T

Y

S

Y

I

S

A

S

D

V

R

L

L

G

N

V

V

D

D

V

F

G

A

L

L

F

I

Y

H

K
|
K

R

E

R
|
R

D

K

H

K

S

A

T

-

I

-

V

-

N

-

G

-

K

-

N
|
N

P

E

I

V

V

D

K

R

H

M

E

V

Y

L

M

V

G

G

R

-

D

D

V

V

E

K

N

D

Q

R

D

V

V

A

L

I

I

L

V

V

D
|
D

M

M

I

A

A
x
D

S
x
T

G
x
C

E
x
G

S
x
T

V

I

L

C

D

H

I

A

A

A

K

D

E

K

L

L

K

L

A

S

R

A

K

G

A

A

R

T

N

R

I

V

Y

Y

V

A

A

I

T

L

T

T

F

H

A

G

F

I

F

F

T

S

-

G

E

P

G

A

L

I

E

S

K

R

F

I

Q

-

K

-

Y

-

D

N

N

A

I

C

I

F

S

E

R

A

V

V

Y

V

S

V

T

T

N

N

L

T

T

I

Y

P

I

Q

P

E

P

D

E

K

L

M

E

K

K

H

T

C

E

S

W

K

F

I

R

Q

K

V

V

I

D

D

M

I

S

S

E

M

L

I

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

T

N

H

K

N

D

G

E

E

S

S

I

V

A

S

binding adenosine monophosphate: R95 (= R125), Q96 (= Q126), N199 (= N239)
binding adenosine-5'-triphosphate: F34 (= F51), N36 (= N53), E38 (= E55)
binding phosphate ion: S46 (= S63), R48 (= R65)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H158), D170 (= D204), G172 (= G206), K193 (= K227), R195 (= R229), D219 (= D260), D220 (= D261), D223 (≠ A264), T224 (≠ S265), C225 (≠ G266), G226 (≠ E267), T227 (≠ S268)

8dbeA Human prps1 with adp; hexamer (see paper)
24% identity, 89% coverage: 22:353/371 of query aligns to 12:309/316 of 8dbeA

query
sites
8dbeA

R

Q

E

D

L

L

G

S

Q

Q

K

K

I

I

D

A

N

D

R

R

I

L

K

G

T

L

K

E

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

V

E

T

I

K

R

K

F
|
F

S

S

N
|
N

G

Q

E
|
E

G

T

K

C

V

V

K

E

L

I

S

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

I

V

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

V

E

Q

N

S

H

G

K

C

G

G

P

E

-

I

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

K

-

I

G

A

K

S

A

A

R

S

R

R

I

V

T

T

V

A

I

V

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R
|
R

Q
|
Q

H

D

R
x
K

-
x
D

K

K

G
x
S

R
|
R

E

A

S

P

L

I

D

S

C

A

A

K

L

L

A

V

L

A

Q

N

E

M

L

L

E

S

R

V

L

A

G

G

V

A

Q

D

E

H

V

I

L

I

T

T

F

M

D

D

A

L

H
|
H

D

A

P

S

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

L

V

M

-

S

-

F

-

E
x
D

N

N

L

L

Y
|
Y

P

A

T

E

Y

P

D

A

I

V

V

L

K

K

T

W

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

S

V

E

N

W

K

R

N

N

S

C

M

T

L

I

V

V

I

-

S

S

P

P

D
|
D

T

A

G

G

A

G

M

A

D

K

R

R

A

V

I

T

Y

S

Y

I

S

A

S

D

V

R

L

L

G

N

V

V

D

D

V

F

G

A

L

L

F

I

Y

H

K

K

R

E

R

R

D

K

H

K

S

A

T

-

I

-

V

-

N

-

G

-

K

-

N

N

P

E

I

V

V

D

K

R

H

M

E

V

Y

L

M

V

G

G

R

-

D

D

V

V

E

K

N

D

Q

R

D

V

V

A

L

I

I

L

V

V

D
|
D

M

M

I

A

A
x
D

S
x
T

G

C

E
x
G

S
x
T

V

I

L

C

D

H

I

A

A

A

K

D

E

K

L

L

K

L

A

S

R

A

K

G

A

A

R

T

N

R

I

V

Y

Y

V

A

A

I

T

L

T

T

F

H

A

G

F

I

F

F

T

S

-

G

E

P

G

A

L

I

E

S

K

R

F

I

Q

-

K

-

Y

-

D

N

N

A

I

C

I

F

S

E

R

A

V

V

Y

V

S

V

T

T

N

N

L

T

T

I

Y

P

I

Q

P

E

P

D

E

K

L

M

E

K

K

H

T

C

E

S

W

K

F

I

R

Q

K

V

V

I

D

D

M

I

S

S

E

M

L

I

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

T

N

H

K

N

D

G

E

E

S
|
S

I
x
V

A
x
S

binding adenosine-5'-diphosphate: F34 (= F51), N36 (= N53), E38 (= E55), R95 (= R125), Q96 (= Q126), K98 (≠ R128), K99 (≠ R129), D100 (vs. gap), S102 (≠ G131), R103 (= R132), H129 (= H158), D142 (≠ E172), Y145 (= Y175), S307 (= S351), V308 (≠ I352), S309 (≠ A353)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H158), D170 (= D204), D219 (= D260), D220 (= D261), D223 (≠ A264), T224 (≠ S265), G226 (≠ E267), T227 (≠ S268)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 312

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
24% identity, 89% coverage: 22:353/371 of query aligns to 13:310/318 of P60891

query
sites
P60891

R

Q

E

D

L

L

G
x
S

Q

Q

K

K

I

I

D

A

N

D

R

R

I

L

K

G

T

L

K

E

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

V

E

T

I

K

R

K

F

F

S

S

N

N

G

Q

E

E

G

T

K

C

V

V

K

E

L

I

S

G

E

E

S

S

V

V

R

R

G

G

K

E

D
|
D

I

V

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

V

E

Q

N

S

H

G

K

C

G

G

P

E

-

I

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

K

-

I

G

A

K

S

A

A

R

S

R

R

I

V

T

T

V

A

I

V

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R

R

Q

Q

H

D

R

K

-

D

K

K

G

S

R

R

E

A

S

P

L

I

D

S

C

A

A

K

L

L

A

V

L

A

Q
x
N

E

M

L

L

E

S

R

V

L

A

G

G

V

A

Q

D

E

H

V

I

L

I

T

T

F

M

D

D

A
x
L

H

H

D

A

P
x
S

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

L
x
V

M

-

S

-

F

-

E

D

N
|
N

L

L

Y
|
Y

P

A

T

E

Y

P

D

A

I

V

V

L

K

K

T

W

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

S

V

E

N

W

K

R

N

N

S

C

M

T

L

I

V

V

I

-

S

S

P

P

D

D

T

A

G

G

A

G

M

A

D

K

R

R

A

V

I

T

Y

S

Y

I

S

A

S
x
D

V

R

L

L

G

N

V

V

D

D

V

F

G
x
A

L

L

F

I

Y
x
H

K

K

R

E

R

R

D

K

H

K

S

A

T

-

I

-

V

-

N

-

G

-

K

-

N

N

P

E

I

V

V
x
D

K

R

H

M

E

V

Y

L

M

V

G

G

R

-

D

D

V

V

E

K

N

D

Q

R

D

V

V

A

L

I

I

L

V
|
V

D

D

M

M

I

A

A

D

S

T

G

C

E

G

S

T

V

I

L

C

D
x
H

I

A

A

A

K

D

E

K

L

L

K

L

A

S

R

A

K

G

A

A

R

T

N

R

I

V

Y

Y

V

A

A

I

T

L

T

T

F

H

A

G

F

I

F

F

T

S

-

G

E

P

G

A

L

I

E

S

K

R

F

I

Q

-

K

-

Y

-

D

N

N

A

I

C

I

F

S

E

R

A

V

V

Y

V

S

V

T

T

N

N

L

-

T

-

Y

T

I

I

P

P

P

Q

E

E

-

D

-

K

L

M

E

K

K

H

T

C

E

S

W

K

F

I

R

Q

K

V

V

I

D

D

M

I

S

S

E

M

L

I

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

T

N

H

K

N

D

G

E

E

S

S

I

V

A

S

S16 (≠ G25) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D68) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ Q142) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (≠ A157) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ P160) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ L168) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N173) mutation to H: No effect on catalytic activity.
Y146 (= Y175) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ S216) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ G223) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ Y226) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ V242) to H: in a breast cancer sample; somatic mutation
V219 (= V259) to G: in a breast cancer sample; somatic mutation
H231 (≠ D271) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
25% identity, 79% coverage: 3:295/371 of query aligns to 2:265/326 of P9WKE3

query
sites
P9WKE3

S

S

Q

H

D

D

F

W

N

T

Y

D

R

N

N

R

G

K

E

N

L

L

G

M

I

L

V

F

A

A

L

G

E

R

S

A

C

H

R

P

E

E

L

L

G

A

Q

E

K

Q

I

V

D

A

N
x
K

R

E

I

L

K

D

T

V

K

H

R

V

K

T

S

S

E

Q

D

D

S

A

F

R

L

-

I

-

P

-

T

-

Q

-

E

-

I

-

R

E

F

F

S

A

N

N

G

G

E

E

G

I

K

F

V

V

K

R

L

F

S

H

E

E

S

S

V

V

R

R

G

G

K

C

D

D

I

A

Y

F

I

V

L

L

C

-

D

-

V

-

G

-

N

-

Y

Q

S

S

C

C

T

P

Y

A

N

P

M

V

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

-

P

-

D

N

E

R

H

W

F

L

Q

M

D

E

I

Q

K

L

R

I

T

M

V

I

S

D

A

A

I

L

-

K

R

R

G

G

K

S

A

A

R

K

R

R

I

I

T

T

V

A

I

V

M

M

P

P

L

F

L

Y

Y

P

E

Y

S

A

R

R

Q

Q

H

D

R

K

-

H

K

R

G

G

R

R

E

E

S

P

L

I

D

S

C

A

A

R

L

L

A

I

L

A

Q

D

E

L

L

L

E

K

R

T

L

A

G

G

V

A

Q

D

E

R

V

I

L

V

T

T

F

V

D

D

A

L

H

H

D

T

P

D

N

Q

V

I

Q

Q

-

G

-

F

-

D

N

G

A

P

I

V

P

D

L

H

M

M

S

R

F

G

E

Q

N

N

L

L

Y

L

P

T

T

G

Y

Y

D

-

I

-

V

-

K

-

T

-

I

-

M

-

E

-

K

-

N

-

I

I

E

R

V

D

N

N

-

Y

-

P

K

D

N

G

S

N

M

M

L

V

V

V

I

V

S

S

P

P

D

D

T

S

G

G

A

R

M

V

D

R

R

I

A

A

I

E

Y

K

Y

W

S

A

S

D

V

A

L

L

-

G

G

G

V

V

D

P

V

L

G

A

L

F

F

I

Y

H

K

K

R

T

R

R

D

D

H

P

S

R

T

V

I

-

V

-

N

-

G

-

K

P

N

N

P

Q

I

V

V

V

K

S

H

N

E

R

Y

V

M

V

G

G

R

-

D

D

V

V

E

A

N

G

Q

R

D

T

V

C

L

V

I

L

V

I

D

D

M

M

I

I

A

D

S

T

G

G

E

G

S

T

V

I

L

A

D

G

I

A

A

V

K

A

E

L

L

L

K

H

A

N

R

D

K

G

A

A

R

G

N

D

I

V

Y

I

V

I

A

A

T

A

T

T

F

H

A

G

F

V

F

L

T

S

E

D

K29 (≠ N30) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
23% identity, 80% coverage: 51:347/371 of query aligns to 34:297/298 of 6nfeA

query
sites
6nfeA

F
|
F

S

S

N
x
D

G

G

E
|
E

G

T

K

M

V

V

K

E

L

I

S

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

I

V

Y

F

I

V

L

L

C

-

D

-

V

-

G

-

N

Q

Y

S

S

T

C

C

T

A

Y

-

N

-

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

P

P

A

D

N

E

N

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

A

S

D

A

A

I

L

-

R

R

R

G

S

K

S

A

A

R

G

R

R

I

I

T

T

V

A

I

V

M

V

P

P

L

Y

L

F

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R
|
R

-

R

-
x
V

R

R

K

S

G

A

R

R

E

V

S

P

L

I

D

T

C

A

A

K

L

V

A

V

L

A

Q

D

E

M

L

M

E

A

R

S

L

V

G

G

V

I

Q

C

E

R

V

V

L

L

T

T

F

V

D

D

A

L

H
|
H

D

A

P

D

N

Q

V

I

Q

Q

N

G

A

F

I

F

P

-

L

Y

M

M

S

P

F

V

E

D

N

N

L

V

Y
|
Y

P

S

T

T

Y

P

D

V

I

L

V

L

K

E

T

D

I

I

I

T

M

K

E

Q

E

K

K

-

N

-

I

-

E

-

V

-

N

-

K

L

N

N

S

N

M

I

L

M

V

I

I

V

S

S

P

P

D

D

T

V

G

G

A

G

M

V

D

V

R
|
R

A

A

I

R

Y
x
A

Y

V

S

A

S
x
K

V

R

L

L

G

N

-

D

V

A

D

E

V

L

G

S

L

I

F

I

Y

D

K

K

R

R

D

E

H

V

S

M

T

H

I

I

V

I

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

E

V

P

E

A

N

N

Q

K

D

N

V

C

L

I

I

I

V

V

D
|
D

M
x
I

I

V

A
x
D

S
x
T

G
x
A

E

G

S
x
T

V

L

L

C

D

T

I

A

A

A

K

H

E

E

L

L

K

K

A

K

R

N

K

G

A

A

R

K

N

S

I

V

Y

R

V

A

A

Y

T

I

T

T

F

H

A

P

F

V

F

L

T

S

E

G

G

P

L

A

E

V

K

N

F

N

Q

I

K

K

Y

H

Y

S

D

G

D

-

N

-

I

-

I

L

S

D

R

E

V

V

Y

V

S

V

T

T

N

D

L

T

T

I

Y

P

I

L

P

S

P

A

E

E

L

A

E

Q

K

N

T

C

E

E

W

K

F

I

R

R

K

V

V

V

D

S

M

L

S

A

E

D

L

M

I

L

S

A

R

Q

I

A

I

I

H

K

K

R

L

V

N

N

binding adenosine-5'-diphosphate: F34 (= F51), D36 (≠ N53), E38 (= E55), R95 (= R125), Q96 (= Q126), H130 (= H158)
binding adenosine monophosphate: R98 (= R128), V100 (vs. gap), Y146 (= Y175), R175 (= R210), A178 (≠ Y213), K181 (≠ S216)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H158), D213 (= D260), D214 (= D261), I215 (≠ M262), D217 (≠ A264), T218 (≠ S265), A219 (≠ G266), T221 (≠ S268)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
24% identity, 82% coverage: 50:353/371 of query aligns to 32:292/295 of 1dkuA

query
sites
1dkuA

R

R

F

F

S

S

N

D

G

G

E

E

G

V

K

Q

V

I

K

N

L

I

S

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

I

C

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

I

E

Q

N

S

H

T

K

S

G

D

P

P

-

V

D

N

E

E

H

H

F

I

Q

M

D

E

I

L

K

L

R

I

T

M

V

V

S

D

A

A

I

L

-

K

R

R

G

A

K

S

A

A

R

K

R

T

I

I

T

N

V

I

I

V

M

I

P

P

L

Y

Y

G

E

Y

S

A

R
|
R

Q

Q

H

D

R

R

R
x
K

-

A

K

R

G
x
S

R
|
R

E

E

S

P

L

I

D

T

C

A

A

K

L

L

A

F

L

A

Q

N

E

L

L

L

E

E

R

T

L

A

G

G

V

A

Q

T

E

R

V

V

L

I

T

A

F

L

D

D

A

L

H
|
H

D

A

P

P

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

-

I

-
x
D

-

H

L

L

M

M

S

G

F

V

E

P

N

I

L

L

Y

G

P

E

T

Y

Y

F

D

-

I

-

V

-

K

-

T

-

I

-

M

-

E

E

E

G

K

K

N

N

I

L

E

E

V

-

N

-

K

-

N

-

S

D

M

I

L

V

V

I

I

V

S

S

P

P

D

D

T

H

G

G

A

G

M

V

D

T

R

R

A

A

I

R

Y

K

Y

L

S

A

S

D

V

R

L

L

G

K

V

A

D

P

V

I

G

A

L

I

F

I

Y

D

K

K

R

R

R

M

D

N

H

-

S

-

T

-

I

I

V

V

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

N

V

I

E

E

N

G

Q

K

D

T

V

A

L

I

I

L

V

I

D

D

M

I

I

I

A

D

S

T

G

A

E

G

S

T

V

I

L

T

D

L

I

A

A

A

K

N

E

A

L

L

K

V

A

E

R

N

K

G

A

A

R

K

N

E

I

V

Y

Y

V

A

A

C

T

C

T

T

F

H

A

P

F

V

F

L

T

S

-

G

E

P

G

A

L

V

E

E

K

R

F

I

Q

-

K

-

Y

-

D

N

N

S

I

T

I

I

S

K

R

E

V

L

Y

V

S

V

T

T

N

N

L

S

T

I

Y

K

I

L

P

-

E

-

L

-

E

-

K

K

T

I

E

E

W

R

F

F

R

K

K

Q

V

L

D

S

M

V

S

G

E

P

L

L

I

L

S

A

R

E

I

A

I

I

H

I

K

R

L

V

N

H

K

E

D

Q

E

Q

S
|
S

I
x
V

A
x
S

binding methyl phosphonic acid adenosine ester: R94 (= R125), H128 (= H158)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R129), S101 (≠ G131), R102 (= R132), D141 (vs. gap), S290 (= S351), V291 (≠ I352), S292 (≠ A353)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 295

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
23% identity, 80% coverage: 51:347/371 of query aligns to 34:298/299 of 6nfeB

query
sites
6nfeB

F
|
F

S

S

N
x
D

G

G

E
|
E

G

T

K

M

V

V

K

E

L

I

S

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

I

V

Y

F

I

V

L

L

C

-

D

-

V

-

G

-

N

Q

Y

S

S

T

C

C

T

A

Y

-

N

-

M

-

F

-

G

-

I

-

E

-

N

-

H

-

K

-

G

P

P

A

D

N

E

N

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

A

S

D

A

A

I

L

-

R

R

R

G

S

K

S

A

A

R

G

R

R

I

I

T

T

V

A

I

V

M

V

P

P

L

Y

L

F

Y

G

E

Y

S

A

R
|
R

Q
|
Q

H

D

R

R

-

R

-

V

R

R

K

S

G

A

R

R

E

V

S

P

L

I

D

T

C

A

A

K

L

V

A

V

L

A

Q

D

E

M

L

M

E

A

R

S

L

V

G

G

V

I

Q

C

E

R

V

V

L

L

T

T

F

V

D

D

A

L

H
|
H

D

A

P

D

N

Q

V

I

Q

Q

N

G

A

F

I

F

P

-

L

Y

M

M

S

P

F

V

E

D

N

N

L

V

Y

Y

P

S

T

T

Y

P

D

V

I

L

V

L

K

E

T

D

I

I

I

T

M

K

E

Q

E

K

K

-

N

-

I

-

E

-

V

-

N

-

K

L

N

N

S

N

M

I

L

M

V

I

I

V

S

S

P

P

D

D

T

V

G

G

A

G

M

V

D

V

R

R

A

A

I

R

Y

A

Y

V

S

A

S

K

V

R

L

L

G

N

-

D

V

A

D

E

V

L

G

S

L

I

F

I

Y

D

K

K

R

R

D

S

H

E

S

V

T

M

I

H

V

I

N

I

G

G

K

E

N

P

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

-

V

-

E

A

N

N

Q

K

D

N

V

C

L

I

I

I

V

V

D
|
D

M
x
I

I

V

A
x
D

S
x
T

G
x
A

E

G

S
x
T

V

L

L

C

D

T

I

A

A

A

K

H

E

E

L

L

K

K

A

K

R

N

K

G

A

A

R

K

N

S

I

V

Y

R

V

A

A

Y

T

I

T

T

F

H

A

P

F

V

F

L

T

S

E

G

G

P

L

A

E

V

K

N

F

N

Q

I

K

K

Y

H

Y

S

D

G

D

-

N

-

I

-

I

L

S

D

R

E

V

V

Y

V

S

V

T

T

N

D

L

T

T

I

Y

P

I

L

P

S

P

A

E

E

L

A

E

Q

K

N

T

C

E

E

W

K

F

I

R

R

K

V

V

V

D

S

M

L

S

A

E

D

L

M

I

L

S

A

R

Q

I

A

I

I

H

K

K

R

L

V

N

N

binding adenosine-5'-diphosphate: F34 (= F51), D36 (≠ N53), E38 (= E55), R95 (= R125), Q96 (= Q126), H130 (= H158)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H158), D214 (= D260), D215 (= D261), I216 (≠ M262), D218 (≠ A264), T219 (≠ S265), A220 (≠ G266), T222 (≠ S268)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
24% identity, 89% coverage: 22:353/371 of query aligns to 12:302/309 of 8dbgA

query
sites
8dbgA

R

Q

E

D

L

L

G

S

Q

Q

K

K

I

I

D

A

N

D

R

R

I

L

K

G

T

L

K

E

R

-

K

-

S

-

E

-

D

-

S

-

F

-

L

L

I

G

P

K

T

V

Q

V

E

T

I

K

R

K

F
|
F

S

S

N
|
N

G

Q

E
|
E

G

T

K

C

V

V

K

E

L

I

S

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

I

V

Y

Y

I

I

L

-

C

-

D

-

V

-

G

-

N

-

Y

-

S

-

C

-

T

-

Y

-

N

-

M

-

F

-

G

-

I

V

E

Q

N

S

H

G

K

C

G

G

P

E

-

I

D

N

E

D

H

N

F

L

Q

M

D

E

I

L

K

L

R

I

T

M

V

I

S

N

A

A

I

C

R

K

-

I

G

A

K

S

A

A

R

S

R

R

I

V

T

T

V

A

I

V

M

I

P

P

L

C

L

F

Y

P

E

Y

S

A

R
|
R

Q
|
Q

H

D

R
x
K

R

K

-

D

K

K

G

S

R

R

E

A

S

P

L

I

D

S

C

A

A

K

L

L

A

V

L

A

Q

N

E

M

L

L

E

S

R

V

L

A

G

G

V

A

Q

D

E

H

V

I

L

I

T

T

F

M

D

D

A

L

H
|
H

D

A

P

S

N

Q

V

I

Q

Q

N

G

-

F

A

D

I

I

P

P

L

V

M

-

S

-

F

-

E

D

N

N

L

L

Y

Y

P

A

T

E

Y

P

D

A

I

V

V

L

K

K

T

W

I

I

I

-

M

-

E

-

E

R

K

E

N

N

I

I

E

S

V

E

N

W

K

R

N

N

S

C

M

T

L

I

V

V

I

-

S

S

P

P

D

D

T

A

G

G

A

G

M

A

D

K

R

R

A

V

I

T

Y

S

Y

I

S

A

S

D

V

R

L

L

G

N

V

V

D

D

V

F

G

A

L

L

F

I

Y

H

K

K

R

-

R

E

D

D

H

R

S

M

T

V

I

L

V

V

N

G

G

-

K

-

N

-

P

-

I

-

V

-

K

-

H

-

E

-

Y

-

M

-

G

-

R

-

D

D

V

V

E

K

N

D

Q

R

D

V

V

A

L

I

I

L

V

V

D

D

M

M

I

A

A
x
D

S
x
T

G
x
C

E

G

S
x
T

V

I

L

C

D

H

I

A

A

A

K

D

E

K

L

L

K

L

A

S

R

A

K

G

A

A

R

T

N

R

I

V

Y

Y

V

A

A

I

T

L

T

T

F

H

A

G

F

I

F

F

T

S

-

G

E

P

G

A

L

I

E

S

K

R

F

I

Q

-

K

-

Y

-

D

N

N

A

I

C

I

F

S

E

R

A

V

V

Y

V

S

V

T

T

N

N

L

-

T

-

Y

T

I

I

P

P

P

Q

E

E

-

D

-

K

L

M

E

K

K

H

T

C

E

S

W

K

F

I

R

Q

K

V

V

I

D

D

M

I

S

S

E

M

L

I

I

L

S

A

R

E

I

A

I

I

H

R

K

R

L

T

N

H

K

N

D

G

E

E

S

S

I

V

A

S

binding adenosine-5'-triphosphate: F34 (= F51), N36 (= N53), E38 (= E55), R95 (= R125), Q96 (= Q126), K98 (≠ R128), H129 (= H158)
binding phosphate ion: S46 (= S63), R48 (= R65), D216 (≠ A264), T217 (≠ S265), C218 (≠ G266), T220 (≠ S268)

Query Sequence

>WP_010964137.1 NCBI__GCF_000008765.1:WP_010964137.1
MNSQDFNYRNGELGIVALESCRELGQKIDNRIKTKRKSEDSFLIPTQEIRFSNGEGKVKL
SESVRGKDIYILCDVGNYSCTYNMFGIENHKGPDEHFQDIKRTVSAIRGKARRITVIMPL
LYESRQHRRKGRESLDCALALQELERLGVQEVLTFDAHDPNVQNAIPLMSFENLYPTYDI
VKTIIMEEKNIEVNKNSMLVISPDTGAMDRAIYYSSVLGVDVGLFYKRRDHSTIVNGKNP
IVKHEYMGRDVENQDVLIVDDMIASGESVLDIAKELKARKARNIYVATTFAFFTEGLEKF
QKYYDDNIISRVYSTNLTYIPPELEKTEWFRKVDMSELISRIIHKLNKDESIAKFMDATY
VLHELLNSRKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory