SitesBLAST
Comparing WP_010964145.1 NCBI__GCF_000008765.1:WP_010964145.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
54% identity, 100% coverage: 2:287/287 of query aligns to 3:285/285 of 3q94A
- active site: D85 (= D84), H86 (= H85), E145 (= E143), H181 (= H177), H209 (= H207), N231 (= N229)
- binding zinc ion: H86 (= H85), E114 (≠ A112), H163 (≠ K160), H181 (= H177), H209 (= H207), E235 (= E233), E239 (≠ V237)
P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
53% identity, 100% coverage: 2:287/287 of query aligns to 3:285/285 of P13243
- T212 (= T210) modified: Phosphothreonine
- T234 (= T232) modified: Phosphothreonine
4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
53% identity, 100% coverage: 2:287/287 of query aligns to 2:277/279 of 4to8A
1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
42% identity, 100% coverage: 1:287/287 of query aligns to 1:305/305 of 1rv8B
- active site: D80 (= D84), H81 (= H85), E140 (= E143), H178 (= H177), H208 (= H207), N251 (= N229)
- binding cobalt (ii) ion: H81 (= H85), E132 (= E135), H178 (= H177), H208 (= H207)
- binding sulfate ion: R116 (≠ K119), H123 (≠ N126), S211 (≠ T210), D253 (≠ N231), T254 (= T232)
8q59A Crystal structure of metal-dependent class ii sulfofructose phosphate aldolase from yersinia aldovae in complex with sulfofructose phosphate (yasqia-zn-sfp) (see paper)
39% identity, 99% coverage: 3:287/287 of query aligns to 4:276/280 of 8q59A
- binding (3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-oxidanylidene-6-phosphonooxy-hexane-1-sulfonic acid: G50 (≠ S49), Q51 (≠ E50), K52 (≠ G51), D82 (= D84), H83 (= H85), H172 (= H177), G173 (= G178), H200 (= H207), G201 (= G208), S203 (≠ T210), N222 (= N229), D224 (≠ N231), T225 (= T232)
- binding zinc ion: H83 (= H85), H172 (= H177), H200 (= H207)
8q5aA Crystal structure of metal-dependent class ii sulfofructosephosphate aldolase from hafnia paralvei hpsqia-zn in complex with dihydroxyacetone phosphate (dhap) (see paper)
39% identity, 99% coverage: 3:287/287 of query aligns to 4:276/276 of 8q5aA
A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
38% identity, 100% coverage: 2:287/287 of query aligns to 3:307/323 of A8B2U2
- S50 (= S49) binding beta-D-fructose 1,6-bisphosphate
- D83 (= D84) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
- H84 (= H85) binding Zn(2+)
- H178 (= H177) binding beta-D-fructose 1,6-bisphosphate; binding Zn(2+)
- G179 (= G178) binding beta-D-fructose 1,6-bisphosphate
- K182 (≠ P181) binding beta-D-fructose 1,6-bisphosphate
- H210 (= H207) binding Zn(2+)
- G211 (= G208) binding beta-D-fructose 1,6-bisphosphate
- S213 (≠ T210) binding beta-D-fructose 1,6-bisphosphate
- N253 (= N229) binding beta-D-fructose 1,6-bisphosphate
- D255 (≠ N231) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
- S256 (≠ T232) binding beta-D-fructose 1,6-bisphosphate
- R259 (≠ Q235) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
- D278 (= D258) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
- R280 (= R260) binding beta-D-fructose 1,6-bisphosphate
P0AB74 D-tagatose-1,6-bisphosphate aldolase subunit KbaY; TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Ketose 1,6-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase; EC 4.1.2.40 from Escherichia coli (strain K12) (see paper)
38% identity, 99% coverage: 2:286/287 of query aligns to 3:283/286 of P0AB74
- D82 (= D84) active site, Proton donor
- H83 (= H85) binding Zn(2+)
- H180 (= H177) binding Zn(2+)
- H208 (= H207) binding Zn(2+)
1gvfB Structure of tagatose-1,6-bisphosphate aldolase (see paper)
37% identity, 99% coverage: 2:286/287 of query aligns to 2:274/275 of 1gvfB
- active site: D81 (= D84), H82 (= H85), H171 (= H177), H199 (= H207), N221 (= N229)
- binding phosphoglycolohydroxamic acid: D81 (= D84), H82 (= H85), H171 (= H177), G172 (= G178), H199 (= H207), G200 (= G208), S202 (≠ T210), N221 (= N229), V222 (= V230), A223 (≠ N231), T224 (= T232)
- binding zinc ion: H82 (= H85), H171 (= H177), H199 (= H207)
3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
38% identity, 100% coverage: 2:287/287 of query aligns to 2:303/319 of 3gayA
- binding 1,6-di-O-phosphono-D-tagatose: N23 (= N23), S49 (= S49), D82 (= D84), H174 (= H177), G175 (= G178), K178 (≠ P181), H206 (= H207), G207 (= G208), S209 (≠ T210), N249 (= N229), D251 (≠ N231), S252 (≠ T232), R255 (≠ Q235)
- binding zinc ion: H83 (= H85), H174 (= H177), H206 (= H207)
3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
37% identity, 100% coverage: 1:286/287 of query aligns to 1:306/307 of 3n9sA
- active site: C69 (≠ K71), E70 (≠ D72), G136 (= G137), H180 (= H177), A226 (vs. gap), N253 (= N229)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E135)
- binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N23), S49 (= S49), D82 (= D84), H83 (= H85), H180 (= H177), G181 (= G178), K184 (≠ P181), H210 (= H207), G211 (= G208), S213 (≠ T210), N253 (= N229), D255 (≠ N231), T256 (= T232)
- binding zinc ion: H83 (= H85), H180 (= H177), H210 (= H207)
3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
37% identity, 100% coverage: 2:287/287 of query aligns to 2:303/319 of 3ohiA
- binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (= S49), D82 (= D84), H83 (= H85), H174 (= H177), G175 (= G178), K178 (≠ P181), G207 (= G208), S209 (≠ T210), N249 (= N229), D251 (≠ N231), S252 (≠ T232), R255 (≠ Q235)
- binding zinc ion: H83 (= H85), H174 (= H177), H206 (= H207)
3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
37% identity, 100% coverage: 2:287/287 of query aligns to 2:302/318 of 3gb6A
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N23), S49 (= S49), H173 (= H177), G174 (= G178), K177 (≠ P181), H205 (= H207), G206 (= G208), S208 (≠ T210), N248 (= N229), D250 (≠ N231), S251 (≠ T232), R254 (≠ Q235)
3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
36% identity, 100% coverage: 1:286/287 of query aligns to 1:296/297 of 3n9rA
- active site: C69 (≠ K71), E70 (≠ D72), G136 (= G137), H170 (= H177), A216 (vs. gap), N243 (= N229)
- binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H85), H170 (= H177), G171 (= G178), K174 (≠ P181), H200 (= H207), G201 (= G208), S203 (≠ T210), N243 (= N229), D245 (≠ N231), T246 (= T232)
- binding zinc ion: H83 (= H85), H170 (= H177), H200 (= H207)
3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
36% identity, 100% coverage: 1:286/287 of query aligns to 1:296/297 of 3c56A
- active site: C69 (≠ K71), E70 (≠ D72), G136 (= G137), H170 (= H177), A216 (vs. gap), N243 (= N229)
- binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N23), S49 (= S49), D82 (= D84), H170 (= H177), K174 (≠ P181), G201 (= G208), S203 (≠ T210), N243 (= N229), D245 (≠ N231), T246 (= T232), R249 (≠ Q235)
- binding zinc ion: H83 (= H85), H170 (= H177), H200 (= H207)
3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
37% identity, 100% coverage: 1:286/287 of query aligns to 1:295/296 of 3c52A
- active site: C69 (≠ K71), E70 (≠ D72), G136 (= G137), H169 (= H177), A215 (vs. gap), N242 (= N229)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E135)
- binding phosphoglycolohydroxamic acid: D82 (= D84), H83 (= H85), H169 (= H177), K173 (≠ P181), H199 (= H207), G200 (= G208), S202 (≠ T210), N242 (= N229), D244 (≠ N231), T245 (= T232)
- binding zinc ion: H83 (= H85), H169 (= H177), H199 (= H207)
5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
36% identity, 100% coverage: 1:286/287 of query aligns to 1:291/292 of 5ucpA
- binding 1,6-di-O-phosphono-D-fructose: S49 (= S49), D82 (= D84), H83 (= H85), H165 (= H177), K169 (≠ P181), G196 (= G208), S198 (≠ T210), N238 (= N229), D240 (≠ N231), T241 (= T232), R244 (≠ Q235)
- binding zinc ion: H83 (= H85), H83 (= H85), H83 (= H85), E134 (= E135), H165 (= H177), H165 (= H177), H165 (= H177), H195 (= H207), H195 (= H207)
5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
36% identity, 100% coverage: 1:286/287 of query aligns to 1:290/291 of 5uckA
- binding glyceraldehyde-3-phosphate: S49 (= S49), D82 (= D84), H83 (= H85), H164 (= H177), D239 (≠ N231), R243 (≠ Q235)
- binding zinc ion: H83 (= H85), H83 (= H85), E134 (= E135), H164 (= H177), H194 (= H207), H194 (= H207)
5ud0A Class ii fructose-1,6-bisphosphate aldolase e149a variant of helicobacter pylori with cleavage products (see paper)
36% identity, 100% coverage: 1:286/287 of query aligns to 1:281/282 of 5ud0A
2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
36% identity, 100% coverage: 2:287/287 of query aligns to 2:292/307 of 2isvB
- binding phosphoglycolohydroxamic acid: D82 (= D84), H168 (= H177), G169 (= G178), K172 (≠ N183), H195 (= H207), G196 (= G208), S198 (≠ T210), N238 (= N229), D240 (≠ N231), S241 (≠ T232)
- binding zinc ion: H83 (= H85), H168 (= H177), H195 (= H207)
Query Sequence
>WP_010964145.1 NCBI__GCF_000008765.1:WP_010964145.1
MLVSAKEMVDKAREGKYAVGQFNINNLEWTKAILLTAQENKSPVILGVSEGAGKYMGGYH
AVVGMVNGLMKDLNITVPVALHLDHGSYEGAFKVIEAGFSSVMFDGSHYDIAENIEKTKE
VIKAANAKGLSVEAEVGAIGGEEDGVVGSGEVADPAECKKIADLGVDILAAGIGNIHGVY
PANWAGLRFDVLEDIKNAVGTMPLVLHGGTGIPDDMIAKAISLGVAKINVNTECQLVFAE
ATRKYIEEGKDKQGKGFDPRKLLAPGFEAIKAKVKEKMVLFGSVNRA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory