SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964257.1 NCBI__GCF_000008765.1:WP_010964257.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

7ac8B Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
35% identity, 99% coverage: 1:201/203 of query aligns to 1:197/202 of 7ac8B

query
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7ac8B

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binding glutamine: G54 (= G50), Q90 (= Q86), E98 (= E94), V142 (= V146), H143 (= H147), T144 (≠ S148), Y145 (= Y149)

1ox4B Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
37% identity, 96% coverage: 5:199/203 of query aligns to 7:211/538 of 1ox4B

query
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1ox4B

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Sites not aligning to the query:

active site: 248, 392
binding pyrophosphate 2-: 463, 488, 511, 512

1ox5A Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
37% identity, 96% coverage: 5:199/203 of query aligns to 7:211/532 of 1ox5A

query
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1ox5A

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Sites not aligning to the query:

active site: 248, 386
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: 283, 285, 313, 346, 347, 384, 386, 424, 451, 456, 457, 482, 505, 506

P33734 Imidazole glycerol phosphate synthase hisHF; IGP synthase; IGPS; ImGP synthase; EC 4.3.2.10; EC 3.5.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 96% coverage: 5:199/203 of query aligns to 4:208/552 of P33734

query
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P33734

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Sites not aligning to the query:

239 mutation R->A,H,K: 1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
258 K→A: No activity. Uncoupling of glutaminase activity from the cyclase activity.; K→R: Small reduction of activity.
360 mutation K->A,R: Almost no effect on activity.

P49915 GMP synthase [glutamine-hydrolyzing]; GMP synthetase; Glutamine amidotransferase; EC 6.3.5.2 from Homo sapiens (Human) (see paper)
27% identity, 62% coverage: 75:199/203 of query aligns to 97:206/693 of P49915

query
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P49915

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C104 (= C82) active site, For GATase activity
H190 (= H184) active site, For GATase activity
E192 (= E186) active site, For GATase activity

Sites not aligning to the query:

337 binding XMP
522 binding XMP
610 binding XMP
685 binding XMP
691 binding XMP

2vxoB Human gmp synthetase in complex with xmp (see paper)
27% identity, 62% coverage: 75:199/203 of query aligns to 75:181/658 of 2vxoB

query
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2vxoB

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active site: C82 (= C82), Y83 (≠ L83), H165 (= H184), E167 (= E186)

Sites not aligning to the query:

active site: 55, 223, 381
binding xanthosine-5'-monophosphate: 302, 348, 349, 404, 405, 406, 489, 575, 610, 650, 654, 655, 656

Query Sequence

>WP_010964257.1 NCBI__GCF_000008765.1:WP_010964257.1
MNKKIAIIDYDMGNLLSVKKAFDYIGANSFITSDSKEIEKSDAIILPGVGAFPDAMSSLK
ENGIDKTIINEAKNGKPFAGICLGMQLLFDESEEVTNTKGLGLIGGKIRKMKTEFKIPHM
GWNSLNIPRECNILKGVSKGSYVYFVHSYYAELADKNNLNAYCDYGTKLPAVVSYKNIFG
IQFHPEKSGEIGLTILKNFWELI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory