SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964260.1 NCBI__GCF_000008765.1:WP_010964260.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
51% identity, 79% coverage: 13:103/115 of query aligns to 14:104/200 of 6j2lA

query
sites
6j2lA

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T

K

D

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G

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L

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M

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binding zinc ion: D79 (= D78), D81 (= D80), D83 (= D82), C96 (= C95), C103 (= C102)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
47% identity, 77% coverage: 13:101/115 of query aligns to 13:101/185 of 6j2lB

query
sites
6j2lB

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K

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binding zinc ion: D78 (= D78), D80 (= D80), D82 (= D82)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 80% coverage: 13:104/115 of query aligns to 15:106/213 of 7bgmA

query
sites
7bgmA

S

N

K

K

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binding zinc ion: D80 (= D78), D82 (= D80), D84 (= D82), C97 (= C95), C104 (= C102)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 80% coverage: 13:104/115 of query aligns to 13:104/204 of 7bgnA

query
sites
7bgnA

S

N

K

K

G

G

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L

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Y

binding adenosine monophosphate: W60 (= W60), K62 (= K62), G63 (= G63), T65 (= T65), S66 (= S66), T94 (≠ A94), H96 (= H96)
binding zinc ion: D78 (= D78), D80 (= D80), D82 (= D82), C95 (= C95), C102 (= C102)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

Query Sequence

>WP_010964260.1 NCBI__GCF_000008765.1:WP_010964260.1
MEYKKILEMMDFSKGLIPAIVQDDENGEVLMLAYMNEEALKRTVDTKRTWFWSRSRKSYW
NKGETSGHFQYVKSIGIDCDGDTILIKVKQVGAACHTGNRSCFYRSFEFEDGGAK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory