SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964316.1 NCBI__GCF_000008765.1:WP_010964316.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 95% coverage: 22:429/429 of query aligns to 22:434/435 of Q5F8J4

query
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Q5F8J4

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L45 (≠ R45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RN 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 99% coverage: 4:427/429 of query aligns to 5:430/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (= N13), V15 (= V14), V45 (= V44), R46 (= R45), R47 (≠ N46), R52 (= R51), I63 (≠ P64), L78 (≠ V79), M79 (≠ I80), P84 (= P85), A102 (= A103), K104 (= K105), G306 (= G302), T310 (= T306)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
45% identity, 82% coverage: 1:351/429 of query aligns to 2:335/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D201), K194 (= K205)
binding lysine: R215 (≠ E226), G219 (≠ K230)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
35% identity, 75% coverage: 1:320/429 of query aligns to 1:308/332 of 6a0tB

query
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6a0tB

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V

-

P

P

R

Q

E

E

L

L

T

R

V

A

D

E

P

P

D

F

D

D

I

L

L

L

E

E

D

A

E

D

S

-

I

-

K

-

I

L

V

V

I

V

E

E

V
x
A

I
x
M

G
|
G

G

G

I

V

N

E

P

A

A

P

K

L

D

R

Y

L

I

V

L

L

R

P

A

A

M

L

K

E

N

A

K

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

M

A

L

L

A

A

T

E

E

A

G

W

D

E

E

S

L

L

F

R

D

P

T

F

A

A

E

E

E

G

G

-

V

-

M

L

F

I

Y

Y

Y

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

H

S

A

F

I

L

N

-

E

E

S

T

L

L

T

R

A

G

N

S

K

E

I

L

E

L

Q

E

L

L

I

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

T

E

L

-

E

K

K

G

M

R

N

T

F

Y

H

A

D

E

A

A

L

L

R

L

E

E

A

A

Q

Q

E

R

K

L

G
|
G

F
x
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

S

L

D
|
D

I

V

E

E

A

G

Y

I

D
|
D

A

A

M

A

Y

H

K
|
K

L

L

A

T

I

L

L

L

S

A

S

R

L

L

S

L

F

V

G

D

T

P

K

G

I

F

N

P

V

F

D

A

D

E

V

V

Y

E

R

A

E

Q

G

G

I

I

T

A

K

R

I

L

E

T

P

P

I

E

D

V

I

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

V

R

I

V

K

R

L

L

V

V

A

A

I

S

V

L

K

F

E

G

T

E

E

G

T

G

G

R

L

W

E

R

M

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

E

Q

R

D

H

H

P

P

L

L

A

A

N

R

V

A

N

R

D

G

S

-

F

-

N

N

A

A

I

L

F

W

I

V

K

R

G

A

N

R

A

P

V

L

G

G

D

E

I

A

M

F

L

V

Y

T

G

G

R

P

G

G

A
|
A

G
|
G

D

G

L

G

P

A

T
|
T

G

A

S

S

A

G

V

L

V

F

A

A

D

D

V

L

I

L

S

R

I

F

L

L

R

S

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: N150 (= N159), G151 (= G160), T152 (= T161), Y178 (≠ F188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (≠ N13), V14 (= V14), L42 (= L43), V43 (= V44), R44 (= R45), D45 (≠ N46), K48 (= K49), R50 (= R51), A73 (≠ V79), M74 (≠ I80), G75 (= G81), A97 (= A103), N98 (= N104), G177 (= G187), E180 (= E190), A289 (= A301), G290 (= G302), T294 (= T306)

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
35% identity, 75% coverage: 1:320/429 of query aligns to 1:308/331 of 6a0sA

query
sites
6a0sA

M

M

D

E

K

A

I

L

K

K

I

I

G

A

I

L

L

L

G

G

L
x
G

G
|
G

N
x
T

V
|
V

G

G

R

S

G

A

V

F

L

Y

K

N

I

L

L

V

N

L

T

E

N

R

K

A

E

E

I

L

Q

S

K

A

R

F

S

G

G

V

Y

V

D

P

I

R

E

F

I

L

G

G

K

-

I

V

L
|
L

V
|
V

R
|
R

N
x
D

I

P

D

R

K
|
K

N

P

R
|
R

G

A

I

I

D

-

V

-

P

P

R

Q

E

E

L

L

T

R

V

A

D

E

P

P

D

F

D

D

I

L

L

L

E

E

D

A

E

D

S

-

I

-

K

-

I

L

V

V

I

V

E

E

V
x
A

I
x
M

G

G

I

V

N

E

P

A

A

P

K

L

D

R

Y

L

I

V

L

L

R

P

A

A

M

L

K

E

N

A

K

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

M

A

L

L

A

A

T

E

E

A

G

W

D

E

E

S

L

L

F

R

D

P

T

F

A

A

E

E

E

G

G

-

V

-

M

L

F

I

Y

Y

Y

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

H

S

A

F

I

L

N

-

E

E

S

T

L

L

T

R

A

G

N

S

K

E

I

L

E

L

Q

E

L

L

I

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

T

E

L

-

E

K

K

G

M

R

N

T

F

Y

H

A

D

E

A

A

L

L

R

L

E

E

A

A

Q

Q

E

R

K

L

G
|
G

F
x
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

S

L

D
|
D

I

V

E

E

A

G

Y

I

D
|
D

A

A

M

A

Y

H

K
|
K

L

L

A

T

I

L

L

L

S

A

S

R

L

L

S

L

F

V

G

D

T

P

K

G

I

F

N

P

V

F

D

A

D

E

V

V

Y

E

R

A

E

Q

G

G

I

I

T

A

K

R

I

L

E

T

P

P

I

E

D

V

I

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

V

R

I

V

K

R

L

L

V

V

A

A

I

S

V

L

K

F

E

G

T

E

E

G

T

G

G

R

L

W

E

R

M

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

E

Q

R

D

H

H

P

P

L

L

A

A

N

R

V

A

N

R

D

G

S

-

F

-

N

N

A

A

I

L

F

W

I

V

K

R

G

A

N

R

A

P

V

L

G

G

D

E

I

A

M

F

L

V

Y

T

G

G

R

P

G

G

A
|
A

G
|
G

D

G

L

G

P

A

T
|
T

G

A

S

S

A

G

V

L

V

F

A

A

D

D

V

L

I

L

S

R

I

F

L

L

R

S

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: K99 (= K105), N150 (= N159), G151 (= G160), T152 (= T161), Y178 (≠ F188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (≠ N13), V14 (= V14), L42 (= L43), V43 (= V44), R44 (= R45), D45 (≠ N46), K48 (= K49), R50 (= R51), A73 (≠ V79), M74 (≠ I80), A97 (= A103), K99 (= K105), G177 (= G187), E180 (= E190), A289 (= A301), G290 (= G302), T294 (= T306)

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
35% identity, 75% coverage: 1:320/429 of query aligns to 1:308/331 of 2ejwA

query
sites
2ejwA

M

M

D

E

K

A

I

L

K

K

I

I

G

A

I

L

L

L

G

G

L

G

G

G

N

T

V

V

G

G

R

S

G

A

V

F

L

Y

K

N

I

L

L

V

N

L

T

E

N

R

K

A

E

E

I

L

Q

S

K

A

R

F

S

G

G

V

Y

V

D

P

I

R

E

F

I

L

G

G

K

-

I

V

L

L

V

V

R

R

N

D

I

P

D

R

K

K

N

P

R

R

G

A

I

I

D

-

V

-

P

P

R

Q

E

E

L

L

T

R

V

A

D

E

P

P

D

F

D

D

I

L

L

L

E

E

D

A

E

D

S

-

I

-

K

-

I

L

V

V

I

V

E

E

V

A

I

M

G

G

I

V

N

E

P

A

A

P

K

L

D

R

Y

L

I

V

L

L

R

P

A

A

M

L

K

E

N

A

K

G

K

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

M

A

L

L

A

A

T

E

E

A

G

W

D

E

E

S

L

L

F

R

D

P

T

F

A

A

E

E

E

G

G

-

V

-

M

L

F

I

Y

Y

Y

H

E
|
E

A

A

S

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

I

L

H

S

A

F

I

L

N

-

E

E

S

T

L

L

T

R

A

G

N

S

K

E

I

L

E

L

Q

E

L

L

I

H

G

G

I

I

I

L

N

N

G

G

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

T

E

L

-

E

K

K

G

M

R

N

T

F

Y

H

A

D

E

A

A

L

L

R

L

E

E

A

A

Q

Q

E

R

K

L

G

G

F

Y

A

A

E

E

A

A

D

D

P

P

T

T

S

L

D

D

I

V

E

E

A

G

Y

I

D
|
D

A

A

M

A

Y

H

K
|
K

L

L

A

T

I

L

L

L

S

A

S

R

L

L

S

L

F

V

G

D

T

P

K

G

I

F

N

P

V

F

D

A

D

E

V

V

Y

E

R

A

E

Q

G

G

I

I

T

A

K

R

I

L

E

T

P

P

I

E

D

V

I

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

V

R

I

V

K

R

L

L

V

V

A

A

I

S

V

L

K

F

E

G

T

E

E

G

T

G

G

R

L

W

E

R

M

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

E

Q

R

D

H

H

P

P

L

L

A

A

N

R

V

A

N

R

D

G

S

-

F

-

N

N

A

A

I

L

F

W

I

V

K

R

G

A

N

R

A

P

V

L

G

G

D

E

I

A

M

F

L

V

Y

T

G

G

R

P

G

G

A

A

G

G

D

G

L

G

P

A

T

T

G

A

S

S

A

G

V

L

V

F

A

A

D

D

V

L

I

L

S

R

I

F

L

L

R

S

G

G

active site: D191 (= D201), K195 (= K205)
binding magnesium ion: E121 (= E129), V124 (= V132), M125 (≠ A133), A126 (≠ G134), T128 (≠ I136)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
31% identity, 74% coverage: 4:319/429 of query aligns to 1:301/304 of F9VNG5

query
sites
F9VNG5

I

M

K

K

I

L

G

L

I

L

L

F

G

G

L
x
Y

G

G

N
|
N

V
|
V

G

G

R

K

G

A

V

F

L

R

K

K

I

L

L

L

N

H

T

-

N

-

K

-

E

-

I

-

Q

E

K

K

R

R

S

S

G

P

Y

-

D

-

I

-

E

E

I

L

G

N

K

D

I

V

L

I

V

I

R

G

N

G

I

I

D

V

K

T

N
x
R

R
|
R

G

G

I

I

-

M

-

L

-

Q

-

D

-

K

-

E

-

D

-

F

-

T

-

P

-

D

-

L

-

E

-

G

D

D

V

V

P

F

R

K

E

A

L

F

L

E

T

K

V

I

D

K

P

P

D

D

D

I

I

I

L

V

E

D

D

V

E
x
S

S

S

I

A

K

N

I

Y

V

-

I

-

E

-

V

-

I

-

G

N

I

G

N

E

P

P

A

S

K

L

D

S

Y

L

I

Y

L

K

R

E

A

A

M

I

K

K

N

D

K

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

M

A

L

P

L

L

A

A

T

L

E

A

G

F

D

N

E

E

L

I

F

F

D

S

T

L

A

A

E

R

E

S

E

K

G

G

V

V

M

K

F

I

Y

G

Y

F

E

Q

A

G

S

T

V

V

A

M

G

S

G

G

I

T

P

P

I

S

I

I

H

N

A

-

I

L

N

Y

E

R

S

V

L

L

T

P

A

G

N

S

K

R

I

V

E

I

Q

K

L

I

I

R

G

G

I

I

I

L

N

N

G

G

T

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

T

N

L

-

E

K

K

G

M

V

N

S

F

F

H

E

D

E

A

A

L

L

R

K

E

E

A

A

Q

Q

E

R

K

R

G
|
G

F

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

S

L

D

D

I

I

E

N

A

G

Y

F

D

D

A

A

M

A

Y

A

K

K

L

I

A

T

I

I

L

L

S

A

S

N

L

F

S

M

F

I

G

G

T

N

K

S

I

V

N

T

V

I

D

K

D

D

V

V

Y

K

R

F

E

E

G

G

I

I

T

N

K

R

I

D

E

L

P

P

I

K

D

N

I

E

E

K

F

-

A

-

R

-

E

-

F

-

G

-

Y

-

V

-

I

I

K

K

L

L

V

I

A

A

I

Y

V

A

K

D

E

E

T

K

E

-

T

-

G

-

L

-

E

E

M

V

R

W

V

V

H

K

P

P

T

L

M

P

I

I

P

S

E

Q

R

D

H

D

P

P

L

L

A

Y

N

N

V

V

N

D

D

G

S

V

F

E

N

N

A

A

I

L

F

E

I

I

K

T

G

T

N

D

A

-

V

I

G

Q

D

S

I

I

M

L

L

I

Y

R

G

G

R

P

G

G

A

A

G
|
G

D

P

L

V

P

N

T

A

G

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

I

I

S

L

I

L

L

K

R

R

Y8 (≠ L11) binding NADP(+)
N10 (= N13) binding NADP(+)
V11 (= V14) binding NADP(+)
R38 (≠ N50) binding NADP(+)
R39 (= R51) binding NADP(+)
S73 (≠ E71) binding NADP(+)
T100 (≠ A103) binding NADP(+)
K102 (= K105) binding NADP(+)
G182 (= G187) binding NADP(+)
E185 (= E190) binding NADP(+)
G284 (= G302) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
31% identity, 74% coverage: 4:319/429 of query aligns to 1:297/300 of 7f4cA

query
sites
7f4cA

I

M

K

K

I

L

G

L

I

L

L
x
F

G
|
G

L
x
Y

G
|
G

N
|
N

V
|
V

G

G

R

K

G

A

V

F

L

R

K

K

I

L

L

L

N

H

T

-

N

-

K

-

E

-

I

-

Q

E

K

K

R

R

S

S

G

P

Y

-

D

-

I

-

E

E

I

L

G

N

K

D

I

V

L

I

V

I

R

G

N

G

I

I

D

V

K
x
T

N
x
R

R
|
R

G

G

I

I

-

M

-

L

-

Q

-

D

-

K

-

E

-

D

-

F

-

T

-

P

-

D

-

L

-

E

-

G

D

D

V

V

P

F

R

K

E

A

L

F

L

E

T

K

V

I

D

K

P

P

D

D

D

I

I

I

L

V

E

D

D
x
V

E
x
S

S
|
S

I

A

K

N

I

Y

V

-

I

-

E

-

V

-

I

-

G

N

I

G

N

E

P

P

A

S

K

L

D

S

Y

L

I

Y

L

K

R

E

A

A

M

I

K

K

N

D

K

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

M

A

L

P

L

L

A

A

T

L

E

A

G

F

D

N

E

E

L

I

F

F

D

S

T

L

A

A

E

R

E

S

E

K

G

G

V

V

M

K

F

I

Y

G

Y

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

H

N

A

-

I

L

N

Y

E

R

S

V

L

L

T

P

A

G

N

S

K

R

I

V

E

I

Q

K

L

I

I

R

G

G

I

I

I

L

N

N

G

G

T

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

T

N

L

-

E

K

K

G

M

V

N

S

F

F

H

E

D

E

A

A

L

L

R

K

E

E

A

A

Q

Q

E

R

K

R

G

G

F

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

S

L

D

D

I

I

E

N

A

G

Y

F

D

D

A

A

M

A

Y

A

K

K

L

I

A

T

I

I

L

L

S

A

S

N

L

F

S

M

F

I

G

G

T

N

K

S

I

V

N

T

V

I

D

K

D

D

V

V

Y

K

R

F

E

E

G

G

I

I

T

N

K

R

I

-

E

-

P

-

I

-

D

D

I

L

E

K

F

-

A

-

R

-

E

-

F

-

G

-

Y

-

V

-

I

I

K

K

L

L

V

I

A

A

I

Y

V

A

K

D

E

E

T

K

E

-

T

-

G

-

L

-

E

E

M

V

R

W

V

V

H

K

P

P

T

L

M

P

I

I

P

S

E

Q

R

D

H

D

P

P

L

L

A

Y

N

N

V

V

N

D

D

G

S

V

F

E

N

N

A

A

I

L

F

E

I

I

K

T

G

T

N

D

A

-

V

I

G

Q

D

S

I

I

M

L

L

I

Y

R

G

G

R

P

G

G

A

A

G
|
G

D

P

L

V

P

N

T
x
A

G

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

I

I

S

L

I

L

L

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ L9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (= N13), V11 (= V14), T37 (≠ K49), R38 (≠ N50), R39 (= R51), V72 (≠ D70), S73 (≠ E71), S74 (= S72), T100 (≠ A103), K102 (= K105), G127 (≠ A130), S131 (≠ G134), E185 (= E190), G280 (= G302), A284 (≠ T306)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
31% identity, 74% coverage: 4:319/429 of query aligns to 1:299/302 of 5x9dA

query
sites
5x9dA

I

M

K

K

I

L

G

L

I

L

L
x
F

G
|
G

L
x
Y

G
|
G

N
|
N

V
|
V

G

G

R

K

G

A

V

F

L

R

K

K

I

L

L

L

N

H

T

-

N

-

K

-

E

-

I

-

Q

E

K

K

R

R

S

S

G

P

Y

-

D

-

I

-

E

E

I

L

G

N

K

D

I

V

L

I

V

I

R

G

N

G

I

I

D

V

K
x
T

N
x
R

R

R

G

G

I

I

-

M

-

L

-

Q

-

D

-

K

-

E

-

D

-

F

-

T

-

P

-

D

-

L

-

E

-

G

D

D

V

V

P

F

R

K

E

A

L

F

L

E

T

K

V

I

D

K

P

P

D

D

D

I

I

I

L

V

E

D

D
x
V

E
x
S

S
|
S

I

A

K

N

I

Y

V

-

I

-

E

-

V

-

I

-

G

N

I

G

N

E

P
|
P

A

S

K

L

D

S

Y

L

I

Y

L

K

R

E

A

A

M

I

K

K

N

D

K

G

K

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

M

A

L

P

L

L

A

A

T

L

E

A

G

F

D

N

E

E

L

I

F

F

D

S

T

L

A

A

E

R

E

S

E

K

G

G

V

V

M

K

F

I

Y

G

Y

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

H

N

A

-

I

L

N

Y

E

R

S

V

L

L

T

P

A

G

N

S

K

R

I

V

E

I

Q

K

L

I

I

R

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

T

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

T

N

L

-

E

K

K

G

M

V

N

S

F

F

H

E

D

E

A

A

L

L

R

K

E

E

A

A

Q

Q

E

R

K

R

G

G

F
x
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

S

L

D
|
D

I

I

E

N

A

G

Y

F

D
|
D

A

A

M

A

Y

A

K
|
K

L

I

A

T

I

I

L

L

S

A

S

N

L

F

S

M

F

I

G

G

T

N

K

S

I

V

N

T

V

I

D

K

D

D

V

V

Y

K

R

F

E

E

G

G

I

I

T

N

K

R

I

-

E

D

P

P

I

K

D

N

I

E

E

K

F

-

A

-

R

-

E

-

F

-

G

-

Y

-

V

-

I

I

K

K

L

L

V

I

A

A

I

Y

V

A

K

D

E

E

T

K

E

-

T

-

G

-

L

-

E

E

M

V

R

W

V

V

H

K

P

P

T

L

M

P

I

I

P

-

E

S

R

Q

H

D

P

P

L

L

A

Y

N

N

V

V

N

D

D

G

S

V

F

E

N

N

A

A

I

L

F

E

I

I

K

T

G

T

N

D

A

-

V

I

G

Q

D

S

I

I

M

L

L

I

Y

R

G

G

R

P

G

G

A
|
A

G
|
G

D

P

L

V

P

N

T
x
A

G

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

I

I

S

L

I

L

L

K

R

R

active site: D196 (= D201), K200 (= K205)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ L9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (= N13), V11 (= V14), T37 (≠ K49), R38 (≠ N50), V72 (≠ D70), S73 (≠ E71), S74 (= S72), P82 (= P85), T100 (≠ A103), N101 (= N104), K102 (= K105), G127 (≠ A130), S131 (≠ G134), N155 (= N159), G156 (= G160), T157 (= T161), Y183 (≠ F188), A184 (= A189), E185 (= E190), D191 (= D196), D196 (= D201), K200 (= K205), A281 (= A301), G282 (= G302), A286 (≠ T306)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 73% coverage: 3:315/429 of query aligns to 2:309/319 of 4xb2A

query
sites
4xb2A

K

K

I

V

K

N

I

I

G

S

I

I

L
x
F

G

G

L
x
F

G
|
G

N
x
T

V
|
V

G

G

R

R

G

A

V

L

L

A

K

E

I

I

L

I

-

A

-

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

N

E

T

L

N

N

K

V

E

I

E

S

I

I

Q

T

K

D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

Y

F

D

D

I

L

E

L

I

E

G

A

K

K

I

E

L

V

V

A

R

E

N

S

I

T

D

G

K

K

N

L

R

S

G

N

I

I

D

G

V

-

P

D

R

Y

E

E

L

V

L

Y

T

N

V

F

D

S

P

P

D

Q

D

E

I

L

L

V

E

E

D

E

E

V

S

K

I

P

K

N

I

I

V

L

I

V

E

D

V
|
V

I

-

G
x
S

I

W

N

D

P

E

A

A

K

H

D

E

Y

M

I

Y

L

K

R

V

A

A

M

L

K

G

N

E

K

G

K

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

M

P

L

P

L

I

A

A

T

N

E

Y

G

Y

D

D

E

E

L

L

F

M

D

N

T

L

A

A

E

K

E

E

E

N

G

N

V

A

M

G

F

I

Y

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

H

G

A

V

I

L

N

R

E

E

S

N

L

L

T

L

A

G

N

E

K

N

I

I

E

K

Q

R

L

I

I

D

G

A

I

V

N

N

G
x
A

T
x
S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

T

S

L

-

E

E

K

G

M

K

N

T

F

L

H

D

D

D

A

A

L

I

R

E

E

E

A

A

Q

K

E

S

K

L

G

G

F

I

A

L

E

E

A

E

D

D

P

P

T

S

S

K

D
|
D

I

I

E

D

A

G

Y

I

D
|
D

A

A

M

Y

Y

Y

K
|
K

L

A

A

K

I

I

L

L

S

H

S

W

L

V

S

S

F

Y

G

G

T

E

K

P

I

P

N

E

V

E

D

E

V

-

Y

-

R

R

E

L

G

G

I

I

T

R

K

E

I

V

E

R

P

D

I

-

D

-

I

-

E

-

F

-

A

A

R

R

E

N

F

-

G

-

Y

-

V

-

I

V

K

R

L

L

V

V

A

A

I

Q

V

V

K

S

E

K

T

G

E

-

T

-

G

-

L

-

E

K

M

I

R

S

V

V

H

K

P

P

T

R

M

K

I

L

P

S

E

S

R

D

H

N

P

P

L

L

A

L

N

-

V

V

N

E

D

G

S

V

F

Q

N

N

A

A

I

A

F

V

I

I

K

R

G

T

N

N

A

N

V

L

G

G

D

E

I

V

M

I

L

L

Y

K

G

G

R

P

G

G

A
x
G

G

G

D

G

L

R

P

V

T
|
T

G

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

I

I

active site: D211 (= D201), K215 (= K205)
binding l-homoserine: A171 (≠ G160), S172 (≠ T161), D206 (= D196), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L9), F10 (≠ L11), G11 (= G12), T12 (≠ N13), V13 (= V14), R40 (= R32), V91 (= V79), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), N115 (= N104), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
30% identity, 73% coverage: 3:315/429 of query aligns to 2:309/319 of 4xb1A

query
sites
4xb1A

K

K

I

V

K

N

I

I

G

S

I

I

L
x
F

G

G

L
x
F

G
|
G

N
x
T

V
|
V

G

G

R

R

G

A

V

L

L

A

K

E

I

I

L

I

-

A

-

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

N

E

T

L

N

N

K

V

E

I

E

S

I

I

Q

T

K
x
D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

Y

F

D

D

I

L

E

L

I

E

G

A

K

K

I

E

L

V

V
x
K

R

E

N

S

I

T

D

G

K

K

N

L

R

S

G

N

I

I

D

G

V

-

P

D

R

Y

E

E

L

V

L

Y

T

N

V

F

D

S

P

P

D

Q

D

E

I

L

L

V

E

E

D

E

E

V

S

K

I

P

K

N

I

I

V

L

I

V

E

D

V
|
V

I

-

G
x
S

I

W

N

D

P

E

A

A

K

H

D

E

Y

M

I

Y

L

K

R

V

A

A

M

L

K

G

N

E

K

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

M

P

L

P

L

I

A

A

T

N

E

Y

G

Y

D

D

E

E

L

L

F

M

D

N

T

L

A

A

E

K

E

E

E

N

G

N

V

A

M

G

F

I

Y

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

H

G

A

V

I

L

N

R

E

E

S

N

L

L

T

L

A

G

N

E

K

N

I

I

E

K

Q

R

L

I

I

D

G

A

I

V

N

N

G

A

T

S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

T

S

L

-

E

E

K

G

M

K

N

T

F

L

H

D

D

D

A

A

L

I

R

E

E

E

A

A

Q

K

E

S

K

L

G

G

F

I

A

L

E

E

A

E

D

D

P

P

T

S

S

K

D

D

I

I

E

D

A

G

Y

I

D
|
D

A

A

M

Y

Y

Y

K
|
K

L

A

A

K

I

I

L

L

S

H

S

W

L

V

S

S

F

Y

G

G

T

E

K

P

I

P

N

E

V

E

D

E

V

-

Y

-

R

R

E

L

G

G

I

I

T

R

K

E

I

V

E

R

P

D

I

-

D

-

I

-

E

-

F

-

A

A

R

R

E

N

F

-

G

-

Y

-

V

-

I

V

K

R

L

L

V

V

A

A

I

Q

V

V

K

S

E

K

T

G

E

-

T

-

G

-

L

-

E

K

M

I

R

S

V

V

H

K

P

P

T

R

M

K

I

L

P

S

E

S

R

D

H

N

P

P

L

L

A

L

N

-

V

V

N

E

D

G

S

V

F

Q

N

N

A

A

I

A

F

V

I

I

K

R

G

T

N

N

A

N

V

L

G

G

D

E

I

V

M

I

L

L

Y

K

G

G

R

P

G

G

A
x
G

G

G

D

G

L

R

P

V

T
|
T

G

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

I

I

active site: D211 (= D201), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L9), F10 (≠ L11), G11 (= G12), T12 (≠ N13), V13 (= V14), D39 (≠ K31), R40 (= R32), K57 (≠ V44), V91 (= V79), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
30% identity, 73% coverage: 3:315/429 of query aligns to 2:309/319 of O58802

query
sites
O58802

K

K

I

V

K

N

I

I

G

S

I

I

L

F

G

G

L
x
F

G

G

N
x
T

V
|
V

G

G

R

R

G

A

V

L

L

A

K

E

I

I

L

I

-

A

-

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

N

E

T

L

N

N

K

V

E

I

E

S

I

I

Q

T

K

D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

Y

F

D

D

I

L

E

L

I

E

G

A

K

K

I

E

L

V

V
x
K

R

E

N

S

I

T

D

G

K

K

N

L

R

S

G

N

I

I

D

G

V

-

P

D

R

Y

E

E

L

V

L

Y

T

N

V

F

D

S

P

P

D

Q

D

E

I

L

L

V

E

E

D

E

E

V

S

K

I

P

K

N

I

I

V

L

I

V

E

D

V

V

I

-

G
x
S

I

W

N

D

P

E

A

A

K

H

D

E

Y

M

I

Y

L

K

R

V

A

A

M

L

K

G

N

E

K

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

M

P

L

P

L

I

A

A

T

N

E

Y

G

Y

D

D

E

E

L

L

F

M

D

N

T

L

A

A

E

K

E

E

E

N

G

N

V

A

M

G

F

I

Y

F

E
|
E

A

S

S

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

I

I

H

G

A

V

I

L

N

R

E

E

S

N

L

L

T

L

A

G

N

E

K

N

I

I

E

K

Q

R

L

I

I

D

G

A

I

V

N

N

G

A

T

S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

T

S

L

-

E

E

K

G

M

K

N

T

F

L

H

D

D

D

A

A

L

I

R

E

E

E

A

A

Q

K

E

S

K

L

G

G

F

I

A

L

E

E

A

E

D

D

P

P

T

S

S

K

D

D

I

I

E

D

A

G

Y

I

D

D

A

A

M

Y

Y

Y

K

K

L

A

A

K

I

I

L

L

S

H

S

W

L

V

S

S

F

Y

G

G

T

E

K

P

I

P

N

E

V

E

D

E

V

-

Y

-

R

R

E

L

G

G

I

I

T

R

K

E

I

V

E

R

P

D

I

-

D

-

I

-

E

-

F

-

A

A

R

R

E

N

F

-

G

-

Y

-

V

-

I

V

K

R

L

L

V

V

A

A

I

Q

V

V

K

S

E

K

T

G

E

-

T

-

G

-

L

-

E

K

M

I

R

S

V

V

H

K

P

P

T

R

M

K

I

L

P

S

E

S

R

D

H

N

P

P

L

L

A

L

N

-

V

V

N

E

D

G

S

V

F

Q

N

N

A

A

I

A

F

V

I

I

K

R

G

T

N

N

A

N

V

L

G

G

D

E

I

V

M

I

L

L

Y

K

G

G

R

P

G

G

A

G

G
|
G

D

G

L

R

P

V

T

T

G

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

I

I

F10 (≠ L11) binding NADPH
T12 (≠ N13) binding NADPH
V13 (= V14) binding NADPH
R40 (= R32) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ V44) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ G81) binding NADPH
S93 (≠ G82) binding NADPH
S114 (≠ A103) binding NADPH
K116 (= K105) binding NADPH
E140 (= E129) binding Na(+)
V143 (= V132) binding Na(+)
A145 (≠ G134) binding Na(+)
T147 (≠ I136) binding Na(+)
G296 (= G302) binding NADPH

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
29% identity, 53% coverage: 3:228/429 of query aligns to 2:236/319 of 3ingA

query
sites
3ingA

K

E

I

I

K

R

I

I

G

I

I

L

L

M

G
|
G

L
x
T

G
|
G

N
|
N

V
|
V

G

G

R

L

G

N

V

V

L

L

K

R

I

I

L

I

N

D

-

A

T

S

N

N

K

R

E

R

I

F

Q

S

K

I

R

K

-

V

-

G

-

V

-

S

-
x
D

-
x
S

-

R

-

S

-

Y

-

A

S

S

G

G

Y

R

D

N

I

L

E

D

I

I

G

S

K

S

I

I

L

-

V

I

R

S

N

N

I
x
K

D

E

K

K

N

T

R

G

G

R

I

I

D

S

V

-

P

-

R

-

E

D

L

R

L

A

T

F

V

S

D

G

P

P

D

E

D

D

I

L

L

M

E

G

D

E

E

A

S

A

I

D

K

L

I

L

V

V

I

D

E
x
C

V
x
T

I
x
P

G

A

G

S

I

R

N

D

P

G

A

V

K

R

D

E

Y

Y

I

S

L

L

-

Y

-

R

R

M

A

A

M

F

K

E

N

S

K

G

K

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

M

S

L

G

L

L

A

A

T

N

E

K

G

W

D

H

E

D

L

I

F

M

D

D

T

S

A

A

E

N

E

Q

E

N

G

S

V

K

M

Y

F

I

Y

R

Y

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

H

S

A

V

I

L

N

D

E

Y

S

S

L

I

T

L

A

P

N

S

K

K

I

V

E

K

Q

R

L

F

I

R

G

G

I

I

I

V

N

S

G

S

T

T

T

I

N

N

Y

Y

I

V

L

I

T

R

K

N

M

M

T

A

L

-

E

N

K

G

M

R

N

S

F

L

H

R

D

D

A

V

L

V

R

D

E

D

A

A

Q

I

E

K

K

K

G

G

F

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

S

D

D

D

I

L

E

N

A

G

Y

L

D
|
D

A

A

M

A

Y

R

K
|
K

L

S

A

V

I

I

L

L

S

V

S

N

L

H

S

I

F

F

G

G

T

T

K

E

I

Y

N

T

V

L

D

N

D

D

V

V

Y

E

R

Y

E

S

G

G

I

V

active site: D209 (= D201), K213 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), T10 (≠ L11), G11 (= G12), N12 (= N13), V13 (= V14), D38 (vs. gap), S39 (vs. gap), K57 (≠ I47), C85 (≠ E78), T86 (≠ V79), P87 (≠ I80), A112 (= A103), N113 (= N104), K114 (= K105), A139 (= A130), E198 (= E190), S199 (≠ A191)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
28% identity, 60% coverage: 1:259/429 of query aligns to 1:256/321 of 3jsaA

query
sites
3jsaA

M

M

D

K

K

T

I

I

K

N

I

L

G

S

I

I

L

F

G
|
G

L
|
L

G
|
G

N
|
N

V
|
V

G

G

R

L

G

N

V

L

L

L

K

R

I

I

L

I

N

R

T

S

N

F

K

N

E

E

-

N

-

R

-

L

-

G

-

L

-

K

-

F

-

N

-

V

-

F

-

V

-

A

-
x
D

-
x
S

I

L

Q

H

K

S

R

Y

S

Y

G

N

Y

E

D

R

I

I

E

D

I

I

G

G

K

K

I

V

L

I

V

S

R

Y

N

-

I

-

D

-

K

K

N

E

R

K

G

G

I

-

D

-

V

-

P

-

R

-

E

S

L

L

L

D

T

S

V

L

D

E

P

Y

D

E

D

S

I

I

L

S

E

A

D

S

E

E

S

A

I

L

K

A

I

R

V

D

I

F

E

D

V

I

I

V

G

V

G

D

I
x
A

N
x
T

P
|
P

A

A

K

S

D

A

-

D

-

G

-

K

-

E

-

L

-

A

Y

F

I

Y

L

K

R

E

A

T

M

F

K

E

N

N

K

G

K

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

M

S

L

G

L

L

A

A

T

N

E

F

G

W

D

P

E

E

L

I

F

M

D

E

T

Y

A

A

E

R

E

S

E

N

G

N

V

R

M

R

F

I

Y

R

Y

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

H

S

A

F

I

I

N

D

E

Y

S

S

L

V

T

L

A

P

N

S

K

R

I

I

E

K

Q

K

L

F

I

R

G

G

I

I

I

V

N

S

G

L

T

T

T

I

N

N

Y

Y

I

F

L

I

T

R

K

E

M

L

T

A

L

-

E

N

K

K

M

R

N

E

F

F

H

D

D

D

A

V

L

L

R

S

E

E

A

A

Q

T

E

K

K

L

G

G

F

I

A

-

E

-

A

V

D

N

P

Y

T

K

S

D

D

D

I

L

E

T

A

G

Y

L

D
|
D

A

A

M

A

Y

R

K
|
K

L

S

A

V

I

I

L

L

S

C

S

N

L

H

S

L

F

Y

G

G

T

S

K

S

I

Y

N

R

V

L

D

S

D

D

V

V

Y

F

R

Y

E

E

G

G

I

I

T

-

K

-

I

-

E

-

P

-

I

-

D

-

I

-

E

-

F

L

A

D

R

R

E

S

F

F

G

G

Y

K

V

N

I

E

K

R

L

L

V

V

A

-

I

-

V

-

K

-

E

-

T

T

E

E

T

T

G

G

L

I

active site: D212 (= D201), K216 (= K205)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (= L11), G12 (= G12), N13 (= N13), V14 (= V14), D42 (vs. gap), S43 (vs. gap), A90 (≠ I83), T91 (≠ N84), P92 (= P85), A117 (= A103), N118 (= N104), A144 (= A130)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 51% coverage: 99:317/429 of query aligns to 667:908/916 of O81852

query
sites
O81852

H

H

V

V

V

I

T

T

A

P

N

N

K

K

M

K

L

A

L

N

A

S

T

G

E

P

G

L

D

D

E

Q

-

Y

-

L

-

K

L

L

F

R

D

D

T

L

A

Q

E

R

E

K

E

S

G

Y

V

T

M

H

F

Y

Y

F

Y

Y

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

H

S

A

T

I

L

N

R

E

G

S

L

L

L

-

E

T

T

A

G

N

D

K

K

I

I

E

L

Q

R

L

I

I

E

G

G

I

I

I

C

N

S

G

G

T

T

T

L

N

S

Y

Y

I

L

L

F

T

N

K

N

M

F

T

V

L

G

E

D

K

R

M

-

N

S

F

F

H

S

D

E

A

V

L

V

R

T

E

E

A

A

Q

K

E

N

K

A

G

G

F

F

A

T

E

E

A

P

D

D

P

P

T

R

S

D

D

D

I

L

E

S

A

G

Y

T

D

D

A

V

M

A

Y

R

K

K

L

V

A

I

I

I

L

L

S

A

S

R

L

E

S

S

F

-

G

G

T

L

K

K

I

L

N

D

V

L

D

A

D

D

V

L

-

P

-

I

-

R

-

S

-

L

-

V

-

P

-

E

-

P

-

L

-

K

-

G

-

C

-

T

-

S

Y

V

R

E

E

E

G

F

I

M

T

E

K

K

I

L

E

P

P

Q

I

Y

D

D

I

G

E

D

F

L

A

A

R

K

E

E

-

R

-

L

-

D

-

A

-

E

-

N

F

S

G

G

Y

E

V

V

I

L

K

R

L

Y

V

V

A

G

I

V

V

V

K

D

E

A

T

V

E

N

T

Q

-

K

-

G

G

T

L

V

E

E

M

L

R

R

V

R

H

Y

P

-

T

-

M

-

I

-

P

K

E

K

R

E

H

H

P

P

L

F

A

A

N

Q

V

L

N

A

D

G

S

S

F

D

N

N

A

I

I

I

F

A

I

F

K

T

G

T

N

T

A

R

V

Y

G

K

D

D

-

H

-

P

I

L

M

I

L

V

Y

R

G

G

R

P

G

G

A

A

G

G

D

A

L

Q

P

V

T

T

G

A

S

G

A

G

V

I

V

F

A

S

D

D

V

I

I

L

S

R

I

L

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
28% identity, 29% coverage: 89:213/429 of query aligns to 100:230/358 of 1tveA

query
sites
1tveA

Y

F

I

Y

L

T

R

K

A

F

M

V

K

E

N

N

K

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

M

K

L

A

L

F

A

S

T

S

E

D

-

L

-

A

-

T

G

W

D

K

E

A

L

L

F

F

D

S

T

N

A

K

E

P

E

T

E

N

G

G

V

F

M

V

F

-

Y

Y

Y

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

H

S

A

F

I

L

N

R

E

E

S

I

L

I

-

Q

T

T

A

G

N

D

K

E

I

V

E

E

Q

K

L

I

I

E

G

G

I

I

I

F

N

S

G

G

T
|
T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

T

S

L

T

E

S

K

Q

M

A

N

N

-

D

-

V

-

K

F

F

H

S

D

D

A

V

L

V

R

K

E

V

A

A

Q

K

E

K

K

L

G

G

F

Y

A

T

E

E

A

P

D

D

P

P

T

R

S

D

D
|
D

I

L

E

N

A

G

Y

L

D
|
D

A

V

M

A

Y

R

K
|
K

L

V

A

T

I

I

L

V

S

G

S

R

L

I

S

S

active site: D218 (= D201), K222 (= K205)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K105), T175 (= T161), D213 (= D196), D218 (= D201)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
28% identity, 29% coverage: 89:213/429 of query aligns to 100:230/358 of 1q7gA

query
sites
1q7gA

Y

F

I

Y

L

T

R

K

A

F

M

V

K

E

N

N

K

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

M

K

L

A

L

F

A

S

T

S

E

D

-

L

-

A

-

T

G

W

D

K

E

A

L

L

F

F

D

S

T

N

A

K

E

P

E

T

E

N

G

G

V

F

M

V

F

-

Y

Y

Y

H

E

E

A
|
A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

H

S

A

F

I

L

N

R

E

E

S

I

L

I

-

Q

T

T

A

G

N

D

K

E

I

V

E

E

Q

K

L

I

I

E

G

G

I

I

I

F

N
x
S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

T

S

L

T

E

S

K

Q

M

A

N

N

-

D

-

V

-

K

F

F

H

S

D

D

A

V

L

V

R

K

E

V

A

A

Q

K

E

K

K

L

G

G

F

Y

A

T

E

E

A

P

D

D

P

P

T

R

S

D

D

D

I

L

E

N

A

G

Y

L

D
|
D

A

V

M

A

Y

R

K
|
K

L

V

A

T

I

I

L

V

S

G

S

R

L

I

S

S

active site: D218 (= D201), K222 (= K205)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A103), K116 (= K105), A143 (= A130), S173 (≠ N159), K222 (= K205)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
28% identity, 29% coverage: 89:213/429 of query aligns to 100:230/358 of 1ebuD

query
sites
1ebuD

Y

F

I

Y

L

T

R

K

A

F

M

V

K

E

N

N

K

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

M

K

L

A

L

F

A

S

T

S

E

D

-

L

-

A

-

T

G

W

D

K

E

A

L

L

F

F

D

S

T

N

A

K

E

P

E

T

E

N

G

G

V

F

M

V

F

-

Y

Y

Y

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

H

S

A

F

I

L

N

R

E

E

S

I

L

I

-

Q

T

T

A

G

N

D

K

E

I

V

E

E

Q

K

L

I

I

E

G

G

I

I

I

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

T

S

L

T

E

S

K

Q

M

A

N

N

-

D

-

V

-

K

F

F

H

S

D

D

A

V

L

V

R

K

E

V

A

A

Q

K

E

K

K

L

G

G

F

Y

A

T

E

E

A

P

D

D

P

P

T

R

S

D

D

D

I

L

E

N

A

G

Y

L

D
|
D

A

V

M

A

Y

R

K
|
K

L

V

A

T

I

I

L

V

S

G

S

R

L

I

S

S

active site: D218 (= D201), K222 (= K205)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K105)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
28% identity, 29% coverage: 89:213/429 of query aligns to 100:230/358 of 1ebfA

query
sites
1ebfA

Y

F

I

Y

L

T

R

K

A

F

M

V

K

E

N

N

K

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K

K

M

K

L

A

L

F

A

S

T

S

E

D

-

L

-

A

-

T

G

W

D

K

E

A

L

L

F

F

D

S

T

N

A

K

E

P

E

T

E

N

G

G

V

F

M

V

F

-

Y

Y

Y

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

H

S

A

F

I

L

N

R

E

E

S

I

L

I

-

Q

T

T

A

G

N

D

K

E

I

V

E

E

Q

K

L

I

I

E

G

G

I

I

I

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

T

S

L

T

E

S

K

Q

M

A

N

N

-

D

-

V

-

K

F

F

H

S

D

D

A

V

L

V

R

K

E

V

A

A

Q

K

E

K

K

L

G

G

F

Y

A

T

E

E

A

P

D

D

P

P

T

R

S

D

D

D

I

L

E

N

A

G

Y

L

D
|
D

A

V

M

A

Y

R

K
|
K

L

V

A

T

I

I

L

V

S

G

S

R

L

I

S

S

active site: D218 (= D201), K222 (= K205)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A103)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
28% identity, 29% coverage: 89:213/429 of query aligns to 101:231/359 of P31116

query
sites
P31116

Y

F

I

Y

L

T

R

K

A

F

M

V

K

E

N

N

K

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K

K

M

K

L

A

L

F

A

S

T

S

E

D

-

L

-

A

-

T

G

W

D

K

E

A

L

L

F

F

D

S

T

N

A

K

E

P

E

T

E

N

G

G

V

F

M

V

F

-

Y

Y

Y

H

E
|
E

A

A

S

T

V
|
V

A

G

G
x
A

G

G

I
x
L

P

P

I

I

H

S

A

F

I

L

N

R

E

E

S

I

L

I

-

Q

T

T

A

G

N

D

K

E

I

V

E

E

Q

K

L

I

I

E

G

G

I

I

I

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

T

S

L

T

E

S

K

Q

M

A

N

N

-

D

-

V

-

K

F

F

H

S

D

D

A

V

L

V

R

K

E

V

A

A

Q

K

E

K

K

L

G

G

F

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

S

D

D

D

I

L

E

N

A

G

Y

L

D
|
D

A

V

M

A

Y

R

K
|
K

L

V

A

T

I

I

L

V

S

G

S

R

L

I

S

S

E143 (= E129) binding Na(+)
V146 (= V132) binding Na(+)
A148 (≠ G134) binding Na(+)
L150 (≠ I136) binding Na(+)
E208 (= E190) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D201) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K205) mutation to V: Loss of activity.

Sites not aligning to the query:

13 binding NAD(+)
15 binding NAD(+)
16 binding NAD(+)
41 binding NAD(+)
79 H→A: Reduces kcat 2-fold.
93 binding NAD(+)
309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

Query Sequence

>WP_010964316.1 NCBI__GCF_000008765.1:WP_010964316.1
MDKIKIGILGLGNVGRGVLKILNTNKEEIQKRSGYDIEIGKILVRNIDKNRGIDVPRELL
TVDPDDILEDESIKIVIEVIGGINPAKDYILRAMKNKKHVVTANKMLLATEGDELFDTAE
EEGVMFYYEASVAGGIPIIHAINESLTANKIEQLIGIINGTTNYILTKMTLEKMNFHDAL
REAQEKGFAEADPTSDIEAYDAMYKLAILSSLSFGTKINVDDVYREGITKIEPIDIEFAR
EFGYVIKLVAIVKETETGLEMRVHPTMIPERHPLANVNDSFNAIFIKGNAVGDIMLYGRG
AGDLPTGSAVVADVISILRGNVDLSAISTVKKNYWNREINPMDKTRSEYYVRITVKDLPG
VLGEISMIFGEEGVSLLSVMQKGKREECVTVVYFTHSANEGMIKSAIDRIKSSKYTKSVD
NIIRIENIQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory