SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010964694.1 NCBI__GCF_000008765.1:WP_010964694.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ij6A Crystal structure of a novel-type phosphoserine phosphatase mutant (h9a) from hydrogenobacter thermophilus tk-6 in complex with l-phosphoserine (see paper)
29% identity, 99% coverage: 1:190/191 of query aligns to 1:195/207 of 4ij6A

query
sites
4ij6A

M

M

L

V

R

K

I

L

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I

L

L

V

V

R
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R

H
x
A

G

A

E

E

T

S

D

E

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W

N
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N

R

P

N

V

K

G

K

R

Y

Y

L
x
Q

G

G

W

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L

D

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D

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I

-

A

-

E

-

A

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E

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H

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Y

Y

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C

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E

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E

D

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E

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F

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Q

G

G

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A

E

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M

V

Y

Y

T

N

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F

M

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L

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D

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S

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A

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C

N

D

N

N

C

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M

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L

L

N

N

active site: R8 (= R8), A9 (≠ H9), N15 (= N15), R58 (= R58), E82 (= E79), H150 (= H145)
binding phosphoserine: R8 (= R8), Q21 (≠ L21), R58 (= R58), E82 (= E79), H85 (≠ F82), H150 (= H145), T151 (≠ G146)

6m1xC Crystal structure of phosphoserine phosphatase in complex with 3- phosphoglyceric acid from entamoeba histolytica (see paper)
32% identity, 100% coverage: 1:191/191 of query aligns to 1:192/196 of 6m1xC

query
sites
6m1xC

M

M

L

T

R

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I

L

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I

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R

H
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H

G

G

E

E

T

T

D

E

S

W

N

N

R

L

N

L

K

G

K

K

Y

I

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x
Q

G

G

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C

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T

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D

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I

E

E

L

L

N

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K

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E

Q

A

A

E

N

M

E

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V

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A

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K

Q

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G

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-

K

N

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F

D

D

F

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P

L

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K

H

R
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R

A

A

K

L

A

I

T

T

A

A

-

Q

K

K

I

I

I

A

R

G

D

D

T

K

N

E

I

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M

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K

E
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E

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W

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N

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Y

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K

D

Y

I

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N

-

Y

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E

D

D

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G

C

C

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W

G

K

M

S

S

F

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A

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S

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E

K

G

N

P

A

K

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E

L

M

L

Y

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K

N

Q

F

N

M

E

N

N

K

K

L

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K

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G

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M

-

E

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G

-

S

T

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H

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G

G

A

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I

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M

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N

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F

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Y

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L

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T

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S

L

F

N

N

D

S

binding 3-phosphoglyceric acid: R8 (= R8), H9 (= H9), Q21 (≠ L21), R57 (= R58), E79 (= E79), H146 (= H145), G147 (= G146)

5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
32% identity, 100% coverage: 1:191/191 of query aligns to 1:192/198 of 5zr2C

query
sites
5zr2C

M

M

L

T

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K

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I

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I

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R

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A

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E

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S

W

N

N

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N

L

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G

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I

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x
Q

G
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G

W

C

T

T

D

D

V

I

E

E

L

L

N

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E

P

K

N

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G

I

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E

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W

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Y

F

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E

E

E

I

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I

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N

-

Y

-

K

-

F

-

L

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S

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G

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E

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D

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G

C

C

E

-

K

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F

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S

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D

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G

K

M

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S

F

I

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A

F

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W

Q

S

G

K

E

K

G

N

P

A

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E

L

M

L

Y

L

T

D

R

L

V

C

S

K

N

Q

F

N

M

E

N

N

K

K

L

-

K

-

G

-

M

-

E

-

G

-

S

T

I

I

L

V

I

C

V

V

T

S

H
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H

G
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G

G

A

I

W

I

I

R

K

S

T

T

S

I

I

A

L

Y

G

L

L

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M

M

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E

T

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M

A

Y

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H

K

F

F

A

Q

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N

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N

N

C

T

G

G

I

I

T

T

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T

I

F

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G

Y

H

A

P

V

V

L

L

K

T

S

S

L

F

N

N

D

S

binding phosphoserine: R8 (= R8), Q21 (≠ L21), G22 (= G22), R57 (= R58), E79 (= E79), H146 (= H145), G147 (= G146)

1h2fA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with trivanadate (see paper)
28% identity, 91% coverage: 6:179/191 of query aligns to 6:185/207 of 1h2fA

query
sites
1h2fA

L

L

V

T

R
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R

H
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H

G

G

E

E

T

T

D

K

S

W

N
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N

R

V

N

E

K

R

Y

M

L
x
Q

G

G

W

W

T

Q

D

D

V

S

E

P

L

L

N

T

E

E

K

K

G

G

I

R

A

Q

E

D

A

A

E

M

M

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K

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A

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T

A

A

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I

I

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L

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E

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G

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x
E

E

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L

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I

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V

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T

H
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H

G
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G

G
x
V

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T

T

L

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M

A

A

Y

A

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E

D

G

H

A

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A

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T

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N

Y

N

G

C

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G

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V

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E

E

V

V

active site: R8 (= R8), H9 (= H9), N15 (= N15), R58 (= R58), E82 (= E79), H150 (= H145)
binding phosphate ion: G142 (= G135), E143 (≠ M136)
binding trivanadate: R8 (= R8), H9 (= H9), N15 (= N15), Q21 (≠ L21), R58 (= R58), E82 (= E79), H150 (= H145), G151 (= G146), V152 (≠ G147)

Sites not aligning to the query:

binding phosphate ion: 1, 2, 3

1h2eA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with phosphate (see paper)
28% identity, 91% coverage: 6:179/191 of query aligns to 6:185/207 of 1h2eA

query
sites
1h2eA

L

L

V

T

R
|
R

H
|
H

G

G

E

E

T

T

D

K

S

W

N
|
N

R

V

N

E

K

R

Y

M

L

Q

G

G

W

W

T

Q

D

D

V

S

E

P

L

L

N

T

E

E

K

K

G

G

I

R

A

Q

E

D

A

A

E

M

M

R

V

L

R

G

D

K

K

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L

L

R

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D

A

T

V

K

E

F

L

D

A

F

A

V

I

I

Y

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S

P

T

L

S

K

G

R
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R

A

A

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L

A

E

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A

A

K

E

I

I

I

V

R

R

D

G

T

G

N

R

I

L

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I

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P

I

I

Y

Y

E

Q

D

D

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E

I

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E

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A

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Y

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N

S

F

I

M

V

N

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K

E

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G

M

E

E

-

G

-

S

T

I

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L

I

I

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T

H
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H

G

G

G

V

I

V

I

L

R

K

S

T

T

L

I

M

A

A

Y

A

L

F

L

K

E

D

M

T

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A

P

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V

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I

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E

E

V

V

active site: R8 (= R8), H9 (= H9), N15 (= N15), R58 (= R58), E82 (= E79), H150 (= H145)
binding phosphate ion: R8 (= R8), H9 (= H9), R58 (= R58), E82 (= E79), H150 (= H145)

6ic0A Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 4 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 231:387/428 of 6ic0A

query
sites
6ic0A

I

I

T

Y

L

L

V

C

R

R

H

H

G

G

E

E

T

N

D

E

S

H

N

N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

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K

R

S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

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A

A

L

L

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N

E
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E

I

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N

E

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Y

K

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D

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Y

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x
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S

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D

D

W
x
K

K

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G

G

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x
Y

K

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R

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V

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S

E

N

P

F

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E

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G
x
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A

A

Y

Y

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E

E

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ L21), E305 (= E79), Y316 (= Y90), R330 (≠ W104), K334 (≠ W108), Y345 (≠ P119), Q371 (≠ G146), R375 (= R150)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-pyrimidin-5-yl-pyridine-4-carboxamide: 28, 29, 32, 33, 135, 137, 146, 149, 197, 200, 224, 226, 404
binding pyrophosphate 2-: 26, 27, 28, 29, 30, 31, 32, 151, 407

6ibzA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 7 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 233:389/431 of 6ibzA

query
sites
6ibzA

I

I

T

Y

L

L

V

C

R

R

H

H

G

G

E

E

T

N

D

E

S

H

N

N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R

S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H

H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

binding 6-O-phosphono-beta-D-fructofuranose: G250 (≠ L21), E307 (= E79), Y318 (= Y90), R332 (≠ W104), K336 (≠ W108), Y347 (≠ P119), Q373 (≠ G146), R377 (= R150)

Sites not aligning to the query:

binding 2-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(pyrimidin-5-ylmethyl)benzenesulfonamide: 30, 34, 35, 137, 139, 144, 148, 152, 199, 202, 226, 228
binding phosphate ion: 28, 29, 30, 31, 32, 33, 153

6hvjA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 3 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 232:388/430 of 6hvjA

query
sites
6hvjA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R236 (= R8), H237 (= H9), N243 (= N15), S286 (≠ R58), E306 (= E79), H371 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: G249 (≠ L21), E306 (= E79), Y317 (= Y90), R331 (≠ W104), K335 (≠ W108), Y346 (≠ P119), Q372 (≠ G146), R376 (= R150)

Sites not aligning to the query:

binding 8-(3-methyl-1-benzofuran-5-yl)-~{N}-(4-methylsulfonylpyridin-3-yl)quinoxalin-6-amine: 29, 33, 34, 136, 138, 143, 147, 151, 198, 225, 407
binding phosphate ion: 28, 29, 30, 31, 32, 152

6hvhA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 1 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 233:389/431 of 6hvhA

query
sites
6hvhA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y
x
I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R237 (= R8), H238 (= H9), N244 (= N15), S287 (≠ R58), E307 (= E79), H372 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: I249 (≠ Y20), G250 (≠ L21), E307 (= E79), Y318 (= Y90), R332 (≠ W104), K336 (≠ W108), Y347 (≠ P119), Q373 (≠ G146), R377 (= R150)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(oxan-4-yl)pyridine-4-carboxamide: 30, 34, 35, 137, 139, 144, 148, 151, 152, 199, 202, 226, 228
binding pyrophosphate 2-: 29, 30, 31, 32, 33, 34, 153, 409

5ajzA Human pfkfb3 in complex with an indole inhibitor 5 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 243:399/440 of 5ajzA

query
sites
5ajzA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R247 (= R8), H248 (= H9), N254 (= N15), S297 (≠ R58), E317 (= E79), H382 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: G260 (≠ L21), E317 (= E79), Y328 (= Y90), R342 (≠ W104), K346 (≠ W108), Y357 (≠ P119), Q383 (≠ G146), R387 (= R150)
binding phosphonic acid: R247 (= R8), H248 (= H9), N254 (= N15), H382 (= H145), Q383 (≠ G146)

Sites not aligning to the query:

binding 2-azanyl-N-[4-[(3-cyano-1H-indol-5-yl)oxy]phenyl]ethanamide: 40, 41, 44, 45, 154, 158, 212, 213, 233, 236, 238

3qpuA Pfkfb3 in complex with ppi (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 241:397/439 of 3qpuA

query
sites
3qpuA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L

G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y

Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W

R

S

E

S

Q

D

D

W

K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P

Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R

R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R245 (= R8), H246 (= H9), N252 (= N15), S295 (≠ R58), E315 (= E79), H380 (= H145)
binding pyrophosphate 2-: R245 (= R8), H246 (= H9), E315 (= E79), H380 (= H145), Q381 (≠ G146)

Sites not aligning to the query:

binding pyrophosphate 2-: 36, 37, 38, 39, 40, 41, 42, 64, 67, 91, 119, 160, 161, 186, 417

6ibxA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 5 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 231:387/429 of 6ibxA

query
sites
6ibxA

I

I

T

Y

L

L

V

C

R

R

H

H

G

G

E

E

T

N

D

E

S

H

N

N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R

S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H

H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ L21), E305 (= E79), Y316 (= Y90), R330 (≠ W104), K334 (≠ W108), Y345 (≠ P119), Q371 (≠ G146), R375 (= R150)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpiperidin-3-yl]pyridine-4-carboxamide: 28, 32, 33, 135, 137, 142, 146, 149, 150, 197, 224
binding pyrophosphate 2-: 27, 28, 29, 30, 31, 32, 151, 407

2dwpA A pseudo substrate complex of 6-phosphofructo-2-kinase of pfkfb (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 234:390/431 of 2dwpA

query
sites
2dwpA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y
x
I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R238 (= R8), H239 (= H9), N245 (= N15), S288 (≠ R58), E308 (= E79), H373 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: I250 (≠ Y20), G251 (≠ L21), E308 (= E79), Y319 (= Y90), R333 (≠ W104), K337 (≠ W108), Y348 (≠ P119), Q374 (≠ G146), R378 (= R150)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 29, 30, 31, 32, 33, 34, 35, 138, 149, 152, 153, 154, 203, 410
binding 6-O-phosphono-beta-D-fructofuranose: 57, 60, 111, 112, 118, 175, 179, 426

6etjA Human pfkfb3 in complex with kan0438241 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 236:392/432 of 6etjA

query
sites
6etjA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y
x
I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R240 (= R8), H241 (= H9), N247 (= N15), S290 (≠ R58), E310 (= E79), H375 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: H241 (= H9), I252 (≠ Y20), G253 (≠ L21), E310 (= E79), Y321 (= Y90), R335 (≠ W104), K339 (≠ W108), Y350 (≠ P119), Q376 (≠ G146), R380 (= R150)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 32, 33, 34, 35, 36, 37, 151, 154, 155, 156, 205, 412
binding 4-[[3-(5-fluoranyl-2-oxidanyl-phenyl)phenyl]sulfonylamino]-2-oxidanyl-benzoic acid: 58, 59, 62, 75, 83, 86, 113, 114, 115, 120, 181

5ak0A Human pfkfb3 in complex with an indole inhibitor 6 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 5ak0A

query
sites
5ak0A

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
binding phosphonic acid: R249 (= R8), H250 (= H9), H384 (= H145), Q385 (≠ G146)

Sites not aligning to the query:

binding (2s)-n-[4-[1-methyl-3-(1-methylpyrazol-4-yl)indol-5-yl]oxyphenyl]pyrrolidine-2-carboxamide: 42, 46, 47, 149, 159, 161, 214, 215, 218, 235, 236, 238

5ajwA Human pfkfb3 in complex with an indole inhibitor 2 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 242:398/439 of 5ajwA

query
sites
5ajwA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R246 (= R8), H247 (= H9), N253 (= N15), S296 (≠ R58), E316 (= E79), H381 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: G259 (≠ L21), E316 (= E79), Y327 (= Y90), R341 (≠ W104), K345 (≠ W108), Y356 (≠ P119), Q382 (≠ G146), R386 (= R150)
binding phosphonic acid: R246 (= R8), H247 (= H9), N253 (= N15), H381 (= H145), Q382 (≠ G146)

Sites not aligning to the query:

binding 2-amino-N-[4-(2-amino-1-benzyl-3-cyano-indol-5-yl)oxyphenyl]acetamide: 39, 40, 43, 44, 153, 157, 208, 211, 212, 215, 232, 233, 235, 237

4d4lA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 4d4lA

query
sites
4d4lA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y
x
I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: I261 (≠ Y20), G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
binding phosphonic acid: R249 (= R8), H250 (= H9), N256 (= N15), H384 (= H145), Q385 (≠ G146)

Sites not aligning to the query:

4d4kA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 242:398/439 of 4d4kA

query
sites
4d4kA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y

I

L

G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R246 (= R8), H247 (= H9), N253 (= N15), S296 (≠ R58), E316 (= E79), H381 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: E316 (= E79), Y327 (= Y90), R341 (≠ W104), K345 (≠ W108), Y356 (≠ P119), Q382 (≠ G146), R386 (= R150)
binding phosphonic acid: R246 (= R8), H247 (= H9), N253 (= N15), H381 (= H145), Q382 (≠ G146)

Sites not aligning to the query:

binding 7-(4-methoxyphenyl)-5-phenyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one: 39, 40, 43, 44, 153, 157, 160, 208, 211, 417

4d4jA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 4d4jA

query
sites
4d4jA

I

I

T

Y

L

L

V

C

R
|
R

H
|
H

G

G

E

E

T

N

D

E

S

H

N
|
N

R

L

N

Q

K

G

K

R

Y
x
I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H
|
H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
binding 6-O-phosphono-beta-D-fructofuranose: I261 (≠ Y20), G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
binding phosphonic acid: R249 (= R8), H250 (= H9), E319 (= E79), H384 (= H145), Q385 (≠ G146)

Sites not aligning to the query:

binding 5-(4-bromophenyl)-7-phenyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one: 42, 43, 46, 47, 156, 160, 163, 211, 214, 235, 238, 240

6ibyA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 6 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 230:386/428 of 6ibyA

query
sites
6ibyA

I

I

T

Y

L

L

V

C

R

R

H

H

G

G

E

E

T

N

D

E

S

H

N

N

R

L

N

Q

K

G

K

R

Y

I

L
x
G

G

G

W

D

T

S

D

G

V

L

E

S

L

S

N

R

E

G

K

K

G

K

I

F

A

A

E

S

A

A

-

L

-

S

E

K

M

F

V

V

R

E

D

E

K

Q

-

N

L

L

R

K

D

D

T

L

K

R

F

-

D

-

F

-

V

V

I

W

S

T

S

S

P

Q

L

L

K

K

R

S

A

T

K

I

A

Q

T

T

A

A

K

E

I

A

I

L

R

R

D

-

T

-

N

-

I

L

I

P

Y

Y

E

E

D

Q

-

W

-

K

A

A

L

L

K

N

E
|
E

I

I

N

D

F

A

G

G

L

V

W

C

D

E

N

E

L

L

S

T

Y
|
Y

K

E

E

E

I

I

K

R

D

D

K

T

Y

Y

P

P

D

E

E

E

C

Y

E

A

K

L

W
x
R

S

E

S

Q

D

D

W
x
K

K

Y

S

Y

F

Y

V

R

F

Y

P

P

Q

T

G

G

E

E

G

S

P
x
Y

K

Q

E

D

M

L

Y

V

T

Q

R

R

V

L

S

E

N

P

F

V

M

I

N

M

K

E

L

L

K

E

G

R

M

Q

E

E

G

-

S

N

I

V

L

L

I

V

V

I

T

C

H

H

G
x
Q

G

A

I

V

I

L

R
|
R

S

C

T

L

I

L

A

A

Y

Y

L

F

L

L

E

D

M

K

G

S

I

A

E

E

binding 6-O-phosphono-beta-D-fructofuranose: G247 (≠ L21), E304 (= E79), Y315 (= Y90), R329 (≠ W104), K333 (≠ W108), Y344 (≠ P119), Q370 (≠ G146), R374 (= R150)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpyrrolidin-3-yl]pyridine-4-carboxamide: 27, 31, 32, 134, 136, 145, 148, 196, 199, 200, 223
binding pyrophosphate 2-: 25, 26, 27, 28, 29, 30, 31, 150, 406

Query Sequence

>WP_010964694.1 NCBI__GCF_000008765.1:WP_010964694.1
MLRITLVRHGETDSNRNKKYLGWTDVELNEKGIAEAEMVRDKLRDTKFDFVISSPLKRAK
ATAKIIRDTNIIYEDALKEINFGLWDNLSYKEIKDKYPDECEKWSSDWKSFVFPQGEGPK
EMYTRVSNFMNKLKGMEGSILIVTHGGIIRSTIAYLLEMGIEGAWHFATNNCGITVIEVR
DSYAVLKSLND

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory