SitesBLAST
Comparing WP_010964694.1 NCBI__GCF_000008765.1:WP_010964694.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4ij6A Crystal structure of a novel-type phosphoserine phosphatase mutant (h9a) from hydrogenobacter thermophilus tk-6 in complex with l-phosphoserine (see paper)
29% identity, 99% coverage: 1:190/191 of query aligns to 1:195/207 of 4ij6A
- active site: R8 (= R8), A9 (≠ H9), N15 (= N15), R58 (= R58), E82 (= E79), H150 (= H145)
- binding phosphoserine: R8 (= R8), Q21 (≠ L21), R58 (= R58), E82 (= E79), H85 (≠ F82), H150 (= H145), T151 (≠ G146)
6m1xC Crystal structure of phosphoserine phosphatase in complex with 3- phosphoglyceric acid from entamoeba histolytica (see paper)
32% identity, 100% coverage: 1:191/191 of query aligns to 1:192/196 of 6m1xC
5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
32% identity, 100% coverage: 1:191/191 of query aligns to 1:192/198 of 5zr2C
1h2fA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with trivanadate (see paper)
28% identity, 91% coverage: 6:179/191 of query aligns to 6:185/207 of 1h2fA
- active site: R8 (= R8), H9 (= H9), N15 (= N15), R58 (= R58), E82 (= E79), H150 (= H145)
- binding phosphate ion: G142 (= G135), E143 (≠ M136)
- binding trivanadate: R8 (= R8), H9 (= H9), N15 (= N15), Q21 (≠ L21), R58 (= R58), E82 (= E79), H150 (= H145), G151 (= G146), V152 (≠ G147)
Sites not aligning to the query:
1h2eA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with phosphate (see paper)
28% identity, 91% coverage: 6:179/191 of query aligns to 6:185/207 of 1h2eA
6ic0A Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 4 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 231:387/428 of 6ic0A
Sites not aligning to the query:
- binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-pyrimidin-5-yl-pyridine-4-carboxamide: 28, 29, 32, 33, 135, 137, 146, 149, 197, 200, 224, 226, 404
- binding pyrophosphate 2-: 26, 27, 28, 29, 30, 31, 32, 151, 407
6ibzA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 7 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 233:389/431 of 6ibzA
Sites not aligning to the query:
- binding 2-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(pyrimidin-5-ylmethyl)benzenesulfonamide: 30, 34, 35, 137, 139, 144, 148, 152, 199, 202, 226, 228
- binding phosphate ion: 28, 29, 30, 31, 32, 33, 153
6hvjA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 3 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 232:388/430 of 6hvjA
- active site: R236 (= R8), H237 (= H9), N243 (= N15), S286 (≠ R58), E306 (= E79), H371 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: G249 (≠ L21), E306 (= E79), Y317 (= Y90), R331 (≠ W104), K335 (≠ W108), Y346 (≠ P119), Q372 (≠ G146), R376 (= R150)
Sites not aligning to the query:
- binding 8-(3-methyl-1-benzofuran-5-yl)-~{N}-(4-methylsulfonylpyridin-3-yl)quinoxalin-6-amine: 29, 33, 34, 136, 138, 143, 147, 151, 198, 225, 407
- binding phosphate ion: 28, 29, 30, 31, 32, 152
6hvhA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 1 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 233:389/431 of 6hvhA
- active site: R237 (= R8), H238 (= H9), N244 (= N15), S287 (≠ R58), E307 (= E79), H372 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: I249 (≠ Y20), G250 (≠ L21), E307 (= E79), Y318 (= Y90), R332 (≠ W104), K336 (≠ W108), Y347 (≠ P119), Q373 (≠ G146), R377 (= R150)
Sites not aligning to the query:
- binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(oxan-4-yl)pyridine-4-carboxamide: 30, 34, 35, 137, 139, 144, 148, 151, 152, 199, 202, 226, 228
- binding pyrophosphate 2-: 29, 30, 31, 32, 33, 34, 153, 409
5ajzA Human pfkfb3 in complex with an indole inhibitor 5 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 243:399/440 of 5ajzA
- active site: R247 (= R8), H248 (= H9), N254 (= N15), S297 (≠ R58), E317 (= E79), H382 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: G260 (≠ L21), E317 (= E79), Y328 (= Y90), R342 (≠ W104), K346 (≠ W108), Y357 (≠ P119), Q383 (≠ G146), R387 (= R150)
- binding phosphonic acid: R247 (= R8), H248 (= H9), N254 (= N15), H382 (= H145), Q383 (≠ G146)
Sites not aligning to the query:
- binding 2-azanyl-N-[4-[(3-cyano-1H-indol-5-yl)oxy]phenyl]ethanamide: 40, 41, 44, 45, 154, 158, 212, 213, 233, 236, 238
3qpuA Pfkfb3 in complex with ppi (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 241:397/439 of 3qpuA
Sites not aligning to the query:
- binding pyrophosphate 2-: 36, 37, 38, 39, 40, 41, 42, 64, 67, 91, 119, 160, 161, 186, 417
6ibxA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 5 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 231:387/429 of 6ibxA
Sites not aligning to the query:
- binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpiperidin-3-yl]pyridine-4-carboxamide: 28, 32, 33, 135, 137, 142, 146, 149, 150, 197, 224
- binding pyrophosphate 2-: 27, 28, 29, 30, 31, 32, 151, 407
2dwpA A pseudo substrate complex of 6-phosphofructo-2-kinase of pfkfb (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 234:390/431 of 2dwpA
- active site: R238 (= R8), H239 (= H9), N245 (= N15), S288 (≠ R58), E308 (= E79), H373 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: I250 (≠ Y20), G251 (≠ L21), E308 (= E79), Y319 (= Y90), R333 (≠ W104), K337 (≠ W108), Y348 (≠ P119), Q374 (≠ G146), R378 (= R150)
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 29, 30, 31, 32, 33, 34, 35, 138, 149, 152, 153, 154, 203, 410
- binding 6-O-phosphono-beta-D-fructofuranose: 57, 60, 111, 112, 118, 175, 179, 426
6etjA Human pfkfb3 in complex with kan0438241 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 236:392/432 of 6etjA
- active site: R240 (= R8), H241 (= H9), N247 (= N15), S290 (≠ R58), E310 (= E79), H375 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: H241 (= H9), I252 (≠ Y20), G253 (≠ L21), E310 (= E79), Y321 (= Y90), R335 (≠ W104), K339 (≠ W108), Y350 (≠ P119), Q376 (≠ G146), R380 (= R150)
Sites not aligning to the query:
- binding adenosine-5'-diphosphate: 32, 33, 34, 35, 36, 37, 151, 154, 155, 156, 205, 412
- binding 4-[[3-(5-fluoranyl-2-oxidanyl-phenyl)phenyl]sulfonylamino]-2-oxidanyl-benzoic acid: 58, 59, 62, 75, 83, 86, 113, 114, 115, 120, 181
5ak0A Human pfkfb3 in complex with an indole inhibitor 6 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 5ak0A
- active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
- binding phosphonic acid: R249 (= R8), H250 (= H9), H384 (= H145), Q385 (≠ G146)
Sites not aligning to the query:
- binding (2s)-n-[4-[1-methyl-3-(1-methylpyrazol-4-yl)indol-5-yl]oxyphenyl]pyrrolidine-2-carboxamide: 42, 46, 47, 149, 159, 161, 214, 215, 218, 235, 236, 238
5ajwA Human pfkfb3 in complex with an indole inhibitor 2 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 242:398/439 of 5ajwA
- active site: R246 (= R8), H247 (= H9), N253 (= N15), S296 (≠ R58), E316 (= E79), H381 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: G259 (≠ L21), E316 (= E79), Y327 (= Y90), R341 (≠ W104), K345 (≠ W108), Y356 (≠ P119), Q382 (≠ G146), R386 (= R150)
- binding phosphonic acid: R246 (= R8), H247 (= H9), N253 (= N15), H381 (= H145), Q382 (≠ G146)
Sites not aligning to the query:
- binding 2-amino-N-[4-(2-amino-1-benzyl-3-cyano-indol-5-yl)oxyphenyl]acetamide: 39, 40, 43, 44, 153, 157, 208, 211, 212, 215, 232, 233, 235, 237
4d4lA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 4d4lA
- active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: I261 (≠ Y20), G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
- binding phosphonic acid: R249 (= R8), H250 (= H9), N256 (= N15), H384 (= H145), Q385 (≠ G146)
Sites not aligning to the query:
- binding 5-(4-chlorophenyl)-7-phenyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one: 42, 43, 46, 47, 156, 160, 163, 211, 214, 240
- binding 6-O-phosphono-beta-D-fructofuranose: 437
4d4kA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 242:398/439 of 4d4kA
- active site: R246 (= R8), H247 (= H9), N253 (= N15), S296 (≠ R58), E316 (= E79), H381 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: E316 (= E79), Y327 (= Y90), R341 (≠ W104), K345 (≠ W108), Y356 (≠ P119), Q382 (≠ G146), R386 (= R150)
- binding phosphonic acid: R246 (= R8), H247 (= H9), N253 (= N15), H381 (= H145), Q382 (≠ G146)
Sites not aligning to the query:
- binding 7-(4-methoxyphenyl)-5-phenyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one: 39, 40, 43, 44, 153, 157, 160, 208, 211, 417
4d4jA Human pfkfb3 in complex with a pyrrolopyrimidone compound
30% identity, 83% coverage: 4:162/191 of query aligns to 245:401/442 of 4d4jA
- active site: R249 (= R8), H250 (= H9), N256 (= N15), S299 (≠ R58), E319 (= E79), H384 (= H145)
- binding 6-O-phosphono-beta-D-fructofuranose: I261 (≠ Y20), G262 (≠ L21), E319 (= E79), Y330 (= Y90), R344 (≠ W104), K348 (≠ W108), Y359 (≠ P119), Q385 (≠ G146), R389 (= R150)
- binding phosphonic acid: R249 (= R8), H250 (= H9), E319 (= E79), H384 (= H145), Q385 (≠ G146)
Sites not aligning to the query:
- binding 5-(4-bromophenyl)-7-phenyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one: 42, 43, 46, 47, 156, 160, 163, 211, 214, 235, 238, 240
6ibyA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 6 (see paper)
30% identity, 83% coverage: 4:162/191 of query aligns to 230:386/428 of 6ibyA
Sites not aligning to the query:
- binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpyrrolidin-3-yl]pyridine-4-carboxamide: 27, 31, 32, 134, 136, 145, 148, 196, 199, 200, 223
- binding pyrophosphate 2-: 25, 26, 27, 28, 29, 30, 31, 150, 406
Query Sequence
>WP_010964694.1 NCBI__GCF_000008765.1:WP_010964694.1
MLRITLVRHGETDSNRNKKYLGWTDVELNEKGIAEAEMVRDKLRDTKFDFVISSPLKRAK
ATAKIIRDTNIIYEDALKEINFGLWDNLSYKEIKDKYPDECEKWSSDWKSFVFPQGEGPK
EMYTRVSNFMNKLKGMEGSILIVTHGGIIRSTIAYLLEMGIEGAWHFATNNCGITVIEVR
DSYAVLKSLND
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory