SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010966437.1 NCBI__GCF_000008765.1:WP_010966437.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1piiA Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from escherichia coli refined at 2.0 angstroms resolution (see paper)
40% identity, 95% coverage: 2:250/262 of query aligns to 4:247/452 of 1piiA

query
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1piiA

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active site: E53 (= E54), K55 (= K56), K114 (= K116), E163 (= E164), N184 (= N185), E214 (= E215), S215 (= S216)
binding phosphate ion: K55 (= K56), S215 (= S216), I235 (= I237), G236 (= G238), S237 (≠ E239)

Sites not aligning to the query:

active site: 260, 379
binding phosphate ion: 386, 407, 427, 428

1jcmP Trpc stability mutant containing an engineered disulphide bridge and in complex with a cdrp-related substrate (see paper)
39% identity, 95% coverage: 2:250/262 of query aligns to 4:247/259 of 1jcmP

query
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1jcmP

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binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: I8 (= I6), E53 (= E54), K55 (= K56), S58 (= S59), P59 (= P60), S60 (= S61), F93 (= F95), K114 (= K116), L188 (= L189), L191 (≠ F192), S215 (= S216), L234 (= L236), I235 (= I237), G236 (= G238), S237 (≠ E239)
binding phosphate ion: R224 (≠ L225), H228 (≠ S229)

6y88B Igps (indole-3-glycerol phosphate synthase) from pseudomonas aeruginosa in complex with substrate inhibitor rcdrp (see paper)
37% identity, 94% coverage: 2:246/262 of query aligns to 5:254/265 of 6y88B

query
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6y88B

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active site: E60 (= E54), K62 (= K56), K122 (= K116), E171 (= E164), N193 (= N185), E223 (= E215), S224 (= S216)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: E60 (= E54), K62 (= K56), S65 (= S59), P66 (= P60), S67 (= S61), F101 (= F95), K122 (= K116), R195 (= R187), L197 (= L189), S224 (= S216), L244 (= L236), V245 (≠ I237), G246 (= G238), E247 (= E239)
binding glycerol: S65 (= S59), P66 (= P60), F100 (≠ Y94)

6y88G Igps (indole-3-glycerol phosphate synthase) from pseudomonas aeruginosa in complex with substrate inhibitor rcdrp (see paper)
40% identity, 80% coverage: 37:246/262 of query aligns to 30:241/253 of 6y88G

query
sites
6y88G

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active site: E51 (= E54), K53 (= K56), K109 (= K116), E158 (= E164), N180 (= N185), E210 (= E215), S211 (= S216)
binding phosphate ion: R182 (= R187), S211 (= S216), G233 (= G238), E234 (= E239)

3t55A Crystal structure of mycobacterium tuberculosis indole glycerol phosphate synthase (igps) in complex with phenoxymethyl benzoic acid (pmba)
33% identity, 97% coverage: 2:255/262 of query aligns to 2:255/258 of 3t55A

query
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3t55A

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I

M

A

K

P

Y

G

V

L

P

P

R

S

D

S

R

V

I

I

I

R

V

I

S

A

E
|
E

S
|
S

S

G

I

V

K

R

T

G

P

T

E

A

D

D

I

L

L

L

Y

A

L

Y

R

A

S

G

I

A

G

G

V

A

N

D

A

A

V

V

L

L

I

V

G

G

E

E

T

G

F

L

M

V

R

T

N

S

I

G

D

D

D

P

L

R

K

A

G

A

I

V

N

A

E

D

F

L

L

V

active site: E53 (= E54), K55 (= K56), K115 (= K116), E164 (= E164), N185 (= N185), E215 (= E215), S216 (= S216)
binding 3-(phenoxymethyl)benzoic acid: S58 (= S59), P59 (= P60), S60 (= S61), F94 (= F95), K115 (= K116), D116 (= D117), F117 (= F118), L136 (= L137), R187 (= R187), L189 (= L189), L192 (≠ F192)

3t44A Crystal structure of mycobacterium tuberculosis indole glycerol phosphate synthase (igps) in complex with indole glycerol phosphate (igp) amd anthranilate
33% identity, 97% coverage: 2:255/262 of query aligns to 2:255/259 of 3t44A

query
sites
3t44A

I

V

L

L

D

D

D

S

I

I

V

L

R

E

D

G

K

V

K

R

L

A

Q

D

L

V

I

A

E

A

D

R

K

E

K

A

A

S

L

V

S

S

L

L

D

S

D

E

I

I

K

K

S

A

K

A

L

A

N

A

S

A

L

A

N

P

L

P

D

-

K

P

R

L

N

D

F

V

K

M

E

A

A

A

L

L

E

R

K

E

E

P

N

G

I

I

S

G

I

V

I

I

A

A

E
|
E

I

V

K
|
K

K

R

A

A

S
|
S

P
|
P

S
|
S

K

A

G

G

V

A

I

L

R

A

E

T

D

I

F

A

N

D

P

P

V

A

K

K

I

L

G

A

Q

Q

I

A

Y

Y

E

Q

N

D

I

G

N

G

I

A

D

R

A

I

V

V

S

S

I

V

L

V

T

T

E

E

K

Q

K

R

Y

R

F
|
F

L

Q

G

G

K

S

N

L

E

D

Y

D

I

L

K

D

I

A

V

V

K

R

E

A

V

S

N

V

S

S

K

I

P

P

I

V

L

L

R

R

K
|
K

D

D

F
|
F

I

V

V

V

D

Q

E

P

Y

Y

Q

Q

L

I

Y

H

E

E

A

A

K

R

L

A

I

H

G

G

A

A

D

D

A

M

V

L

L

L

I

I

A

V

A

A

V

A

L

L

K

E

D

Q

K

S

-

V

L

L

E

V

C

S

F

M

Y

L

N

D

R

R

T

T

L

E

E

S

L

L

G

G

L

M

D

T

S

A

I

L

T

V

E
|
E

V

V

H

H

N

T

E

E

E

Q

E

E

A

A

K

D

L

R

A

A

S

L

E

K

I

A

G

G

C

A

S

K

I

V

I

I

G

G

I

V

N
|
N

N

A

R
|
R

D

D

L
|
L

R

M

D

T

F

L

S

D

T

V

D

D

I

R

T

D

T

C

T

F

K

A

R

R

L

I

M

A

K

P

Y

G

V

L

P

P

R

S

D

S

R

V

I

I

I

R

V

I

S

A

E
|
E

S
|
S

S

G

I

V

K

R

T

G

P

T

E

A

D

D

I

L

L

L

Y

A

L

Y

R

A

S

G

I

A

G

G

V

A

N

D

A

A

V

V

L
|
L

I
x
V

G
|
G

E
|
E

T

G

F

L

M

V

R

T

N

S

I

G

D

D

D

P

L

R

K

A

G

A

I

V

N

A

E

D

F

L

L

V

active site: E53 (= E54), K55 (= K56), K115 (= K116), E164 (= E164), N185 (= N185), E215 (= E215), S216 (= S216)
binding 2-aminobenzoic acid: S58 (= S59), P59 (= P60), S60 (= S61), F94 (= F95), R187 (= R187), L189 (= L189)
binding indole-3-glycerol phosphate: E53 (= E54), K55 (= K56), F94 (= F95), K115 (= K116), F117 (= F118), E164 (= E164), N185 (= N185), E215 (= E215), S216 (= S216), L236 (= L236), V237 (≠ I237), G238 (= G238), E239 (= E239)

1vc4B Crystal structure of indole-3-glycerol phosphate synthase (trpc) from thermus thermophilus at 1.8 a resolution (see paper)
41% identity, 79% coverage: 39:246/262 of query aligns to 36:244/254 of 1vc4B

query
sites
1vc4B

N

S

F

F

K

K

E

E

A

A

L

L

E

L

K

R

E

P

N

G

I

L

S

S

I

V

I

I

A

A

E
|
E

I

V

K
|
K

K

R

A

Q

S

S

P

P

S

S

K

E

G

G

V

L

I

I

R

R

E

E

D

-

F

V

N

D

P

P

V

V

K

E

I

A

G

A

Q

L

I

A

Y

Y

E

A

N

R

I

G

N

G

I

A

D

R

A

A

V

V

S

S

I

V

L

L

T

T

E

E

K

P

K

H

Y

R

F

F

L

G

G

G

K

S

N

L

E

L

Y

D

I

L

K

K

I

R

V

V

K

R

E

E

V

A

N

V

S

D

K

L

P

P

I

L

L

L

R

R

K
|
K

D

D

F

F

I

V

V

V

D

D

E

P

Y

F

Q

M

L

L

Y

E

E

E

A

A

K

R

L

A

I

F

G

G

A

A

D

S

A

A

V

A

L

L

I

I

A

V

A

A

V

L

L

L

K

G

D

E

K

L

L

T

E

G

C

A

F

Y

Y

L

N

E

R

E

T

A

L

R

E

R

L

L

G

G

L

L

D

E

S

A

I

L

T

V

E
|
E

V

V

H

H

N

T

E

E

E

R

E

E

A

L

K

E

L

I

A

A

S

L

E

E

I

A

G

G

C

A

S

E

I

V

I

L

G

G

I

I

N
|
N

N

N

R

R

D

D

L

L

R

A

D

T

F

L

S

H

T

I

D

N

I

L

T

E

T

T

T

A

K

P

R

R

L

L

M

G

K

R

Y
x
L

V

A

P

-

R

R

D

K

R
|
R

-

G

-

F

-

G

I

V

I

L

V

V

S

A

E
|
E

S
|
S

S

G

I

Y

K

S

T

R

P

K

E

E

D

E

I

L

L

K

Y

A

L

L

R

E

S

G

I

L

G

-

V

F

N

D

A

A

V

V

L

L

I

I

G

G

E

T

T

S

F

L

M

M

R

R

N

A

I

P

D

D

active site: E51 (= E54), K53 (= K56), K112 (= K116), E160 (= E164), N181 (= N185), E214 (= E215), S215 (= S216)
binding glycerol: L201 (≠ Y205), R205 (= R210)

3t78A Crystal structure of mycobacterium tuberculosis indole glycerol phosphate synthase (igps) in complex with 5-fluoroanthranilate
33% identity, 97% coverage: 2:255/262 of query aligns to 2:253/257 of 3t78A

query
sites
3t78A

I

V

L

L

D

D

D

S

I

I

V

L

R

E

D

G

K

V

K

R

L

A

Q

D

L

V

I

A

E

A

D

R

K

E

K

A

A

S

L

V

S

S

L

L

D

S

D

E

I

I

K

K

S

A

K

A

L

A

N

A

S

A

L

A

N

P

L

P

D

-

K

P

R

L

N

D

F

V

K

M

E

A

A

A

L

L

E

R

K

E

E

P

N

G

I

I

S

G

I

V

I

I

A

A

E
|
E

I

V

K
|
K

K

R

A

A

S
|
S

P
|
P

S
|
S

K

A

G

G

V

A

I

L

R

A

E

T

D

I

F

A

N

D

P

P

V

A

K

K

I

L

G

A

Q

Q

I

A

Y

Y

E

Q

N

D

I

G

N

G

I

A

D

R

A

I

V

V

S

S

I

V

L

V

T

T

E

E

K

Q

K

R

Y

R

F
|
F

L

Q

G

G

K

S

N

L

E

D

Y

D

I

L

K

D

I

A

V

V

K

R

E

A

V

S

N

V

S

S

K

I

P

P

I

V

L

L

R

R

K
|
K

D

D

F
|
F

I

V

V

V

D

Q

E

P

Y

Y

Q

Q

L

I

Y

H

E

E

A

A

K

R

L

A

I

H

G

G

A

A

D

D

A

M

V

L

L

L

I

I

A

V

A

A

V

A

L

L

K

E

D

Q

K

S

-

V

L

L

E

V

C

S

F

M

Y

L

N

D

R

R

T

T

L

E

E

S

L

L

G

G

L

M

D

T

S

A

I

L

T

V

E
|
E

V

V

H

H

N

T

E

E

E

Q

E

E

A

A

K

D

L

R

A

A

S

L

E

K

I

A

G

G

C

A

S

K

I

V

I

I

G

G

I

V

N
|
N

N

A

R
|
R

D

D

L
|
L

R

M

D

T

F

L

S

D

T

R

D

D

I

-

T

-

T

C

T

F

K

A

R

R

L

I

M

A

K

P

Y

G

V

L

P

P

R

S

D

S

R

V

I

I

I

R

V

I

S

A

E
|
E

S
|
S

S

G

I

V

K

R

T

G

P

T

E

A

D

D

I

L

L

L

Y

A

L

Y

R

A

S

G

I

A

G

G

V

A

N

D

A

A

V

V

L

L

I

V

G

G

E

E

T

G

F

L

M

V

R

T

N

S

I

G

D

D

D

P

L

R

K

A

G

A

I

V

N

A

E

D

F

L

L

V

active site: E53 (= E54), K55 (= K56), K115 (= K116), E164 (= E164), N185 (= N185), E213 (= E215), S214 (= S216)
binding 2-amino-5-fluorobenzoic acid: S58 (= S59), P59 (= P60), S60 (= S61), F94 (= F95), F117 (= F118), R187 (= R187), L189 (= L189)

7etyA Crystal structure of bifunctional indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase (trpc) from corynebacterium glutamicum in complex with reduced 1-(o-carboxyphenylamino)-1- deoxyribulose 5-phosphate (rcdrp) (see paper)
33% identity, 94% coverage: 2:246/262 of query aligns to 4:247/470 of 7etyA

query
sites
7etyA

I

V

L

L

D

E

D

S

I
|
I

V

V

R

E

D

G

K

R

L

G

Q

H

L

L

I

E

E

E

D

I

K

R

K

A

A

R

L

I

S

A

L

H

D

V

D

D

I

V

K

-

S

-

K

-

L

-

N

D

S

A

L

L

N

P

L

K

D

S

K
x
T

R

R

N

S

F

L

K

F

E

D

A

S

L

L

E

N

-

Q

-

G

K

R

E

G

N

G

I

A

S

R

I

F

I

I

A

M

E
|
E

I

C

K
|
K

K

S

A

A

S
|
S

P
|
P

S
|
S

K

L

G

G

V
x
M

I

I

R

R

E

E

D

H

F

Y

N

Q

P

P

V

G

K

E

I

I

G

A

Q

R

I

V

Y

Y

E

S

N

R

I

Y

N

A

I

-

D

S

A

G

V

I

S

S

I

V

L

L

T

C

E

E

K
x
P

K
x
D

Y
x
R

F
|
F

L
x
G

G

G

K

D

N

Y

E

D

Y

H

I

L

K

A

I

T

V

V

K

A

E

A

V

T

N

S

S

H

K

L

P

P

I

V

L

L

R

C

K
|
K

D

D

F

F

I

I

V

I

D

D

E

P

Y

V

Q

Q

L

V

Y

H

E

A

A

A

K
x
R

L

Y

I

F

G

G

A

A

D

D

A

A

V

I

L

L

I

M

A

L

A

S

V

V

L

L

K

D

D

D

K

E

L

E

E

Y

C

A

F

A

Y

L

N

A

R

A

T

E

L

A

-

A

E

R

L

F

G

D

L

L

D

D

S

I

I

L

T

T

E

E

V

V

H

I

N

D

E

E

A

V

K

A

L

R

A

A

S

I

E

K

I

L

G

G

C

A

S

K

I

I

I

F

G

G

I

V

N

N

N

H

R

R

D

N

L
|
L

R

H

D

D

F

L

S

S

T

I

D

D

I

L

T

D

T

R

T

S

K

R

R

R

L

L

M

S

K

K

Y

L

V

I

P

P

R

A

D

D

R

A

I

V

I

L

V

V

S

S

E

E

S
|
S

S

G

I

V

K
x
R

T

D

P

T

E

E

D

T

I

V

L

-

Y

-

L

-

R

R

S

Q

I

L

G

G

-

G

-

H

V

S

N

N

A

A

V

F

L

L

I
x
V

G
|
G

E
x
S

-

Q

-

L

T

T

F

S

M

Q

R

E

N

N

I

V

D

D

binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: I8 (= I6), E54 (= E54), K56 (= K56), S59 (= S59), P60 (= P60), S61 (= S61), F94 (= F95), K115 (= K116), L189 (= L189), S216 (= S216), R219 (≠ K219), V236 (≠ I237), G237 (= G238), S238 (≠ E239)
binding glycerol: T35 (≠ K37), M64 (≠ V64), P91 (≠ K92), D92 (≠ K93), R93 (≠ Y94), G95 (≠ L96), R128 (≠ K129)

Sites not aligning to the query:

binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: 261, 283, 288, 290, 314, 333, 388, 392, 393, 395, 416, 434, 436, 437

7etxA Crystal structure of bifunctional indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase (trpc) from corynebacterium glutamicum (see paper)
33% identity, 94% coverage: 2:246/262 of query aligns to 6:249/472 of 7etxA

query
sites
7etxA

I

V

L

L

D

E

D

S

I

I

V

V

R

E

D

G

K

R

L

G

Q

H

L

L

I

E

E

E

D

I

K

R

K

A

A

R

L

I

S

A

L

H

D

V

D

D

I

V

K

-

S

-

K

-

L

-

N

D

S

A

L

L

N

P

L

K

D

S

K
x
T

R

R

N

S

F

L

K

F

E

D

A

S

L

L

E

N

-

Q

-

G

K

R

E

G

N

G

I

A

S

R

I

F

I

I

A

M

E

E

I

C

K

K

K

S

A

A

S

S

P

P

S

S

K

L

G

G

V

M

I

I

R

R

E

E

D

H

F

Y

N

Q

P

P

V

G

K

E

I

I

G

A

Q

R

I

V

Y

Y

E

S

N

R

I

Y

N

A

I

-

D

S

A

G

V

I

S

S

I

V

L

L

T

C

E

E

K

P

K

D

Y

R

F

F

L

G

G

G

K

D

N

Y

E

D

Y

H

I

L

K

A

I

T

V

V

K

A

E

A

V

T

N

S

S

H

K

L

P

P

I

V

L

L

R

C

K

K

D

D

F

F

I

I

V

I

D

D

E

P

Y

V

Q

Q

L

V

Y

H

E

A

A

A

K
x
R

L

Y

I

F

G

G

A

A

D
|
D

A

A

V

I

L

L

I

M

A

L

A

S

V

V

L

L

K

D

D

D

K

E

L

E

E

Y

C

A

F

A

Y

L

N

A

R

A

T

E

L

A

-

A

E

R

L

F

G

D

L

L

D

D

S

I

I

L

T

T

E

E

V

V

H

I

N

D

E

E

A

V

K

A

L

R

A

A

S

I

E

K

I

L

G

G

C

A

S

K

I

I

I

F

G

G

I

V

N

N

N

H

R

R

D

N

L

L

R

H

D

D

F

L

S

S

T

I

D

D

I

L

T

D

T

R

T

S

K

R

R

R

L

L

M

S

K

K

Y

L

V

I

P

P

R

A

D

D

R

A

I

V

I

L

V

V

S

S

E

E

S

S

S

G

I

V

K

R

T

D

P

T

E

E

D

T

I

V

L

-

Y

-

L

-

R

R

S

Q

I

L

G

G

-

G

-

H

V

S

N

N

A

A

V

F

L

L

I

V

G

G

E

S

-

Q

-

L

T

T

F

S

M

Q

R

E

N

N

I

V

D

D

binding glycerol: T37 (≠ K37), R130 (≠ K129), D135 (= D134)

Sites not aligning to the query:

binding glycerol: 336, 338, 364, 365, 367

3t40A Crystal structure of mycobacterium tuberculosis indole glycerol phosphate synthase (igps) complex with n-2-carboxyphenyl glycine (cpg)
31% identity, 97% coverage: 2:255/262 of query aligns to 2:241/251 of 3t40A

query
sites
3t40A

I

V

L

L

D

D

D

S

I

I

V

L

R

E

D

G

K

V

K

R

L

A

Q

D

L

V

I

A

E

A

D

R

K

E

K

A

A

S

L

V

S

S

L

L

D

S

D

E

I

I

K

K

S

A

K

A

L

A

N

A

S

A

L

A

N

P

L

P

D

-

K

P

R

L

N

D

F

V

K

M

E

A

A

A

L

L

E

R

K

E

E

P

N

G

I

I

S

G

I

V

I

I

A

A

E
|
E

I

V

K
|
K

K

-

A

-

S

-

P

-

S

-

K

-

G

-

V

-

I

-

R

R

E

T

D

I

F

A

N

D

P

P

V

A

K

K

I

L

G

A

Q

Q

I

A

Y

Y

E

Q

N

D

I

G

N

G

I

A

D

R

A

I

V

V

S

S

I

V

L

V

T

T

E

E

K

Q

K

R

Y

R

F
|
F

L

Q

G

G

K

S

N

L

E

D

Y

D

I

L

K

D

I

A

V

V

K

R

E

A

V

S

N

V

S

S

K

I

P

P

I

V

L

L

R

R

K
|
K

D
|
D

F

F

I

V

V

V

D

Q

E

P

Y

Y

Q

Q

L

I

Y

H

E

E

A

A

K

R

L

A

I

H

G

G

A

A

D

D

A

M

V

L

L
|
L

L

L

I

I

A

V

A

A

V

A

L

L

K

E

D

Q

K

S

-

V

L

L

E

V

C

S

F

M

Y

L

N

D

R

R

T

T

L

E

E

S

L

L

G

G

L

M

D

T

S

A

I

L

T

V

E
|
E

V

V

H

H

N

T

E

E

E

Q

E

E

A

A

K

D

L

R

A

A

S

L

E

K

I

A

G

G

C

A

S

K

I

V

I

I

G

G

I

V

N
|
N

N

A

R

R

D

D

L

V

R

-

D

-

F

-

S

-

T

-

D

D

I

R

T

D

T

C

T

F

K

A

R

R

L

I

M

A

K

P

Y

G

V

L

P

P

R

S

D

S

R

V

I

I

I

R

V

I

S

A

E
|
E

S
|
S

S

G

I

V

K

R

T

G

P

T

E

A

D

D

I

L

L

L

Y

A

L

Y

R

A

S

G

I

A

G

G

V

A

N

D

A

A

V

V

L

L

I

V

G

G

E

E

T

G

F

L

M

V

R

T

N

S

I

G

D

D

D

P

L

R

K

A

G

A

I

V

N

A

E

D

F

L

L

V

active site: E53 (= E54), K55 (= K56), K106 (= K116), E155 (= E164), N176 (= N185), E201 (= E215), S202 (= S216)
binding 2-[(carboxymethyl)amino]benzoic acid: E53 (= E54), K55 (= K56), F85 (= F95), K106 (= K116), D107 (= D117), L127 (= L137), E155 (= E164)

1lbfA Crystal structure of indole-3-glycerol phosphate syntase (igps)with reduced 1-(o-caboxyphenylamino)-1-deoxyribulose 5-phosphate (rcdrp) (see paper)
38% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 1lbfA

query
sites
1lbfA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S
|
S

P
|
P

S
|
S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F
|
F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
|
K

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
|
E

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
|
N

N

S

R
|
R

D

D

L
|
L

R

E

D

T

F
x
L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
|
E

S
|
S

S
x
G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L
|
L

I

I

G
|
G

E
x
S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), K109 (= K116), E158 (= E164), N179 (= N185), E209 (= E215), S210 (= S216)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: E50 (= E54), K52 (= K56), S55 (= S59), P56 (= P60), S57 (= S61), F88 (= F95), K109 (= K116), R181 (= R187), L183 (= L189), L186 (≠ F192), S210 (= S216), G211 (≠ S217), L230 (= L236), G232 (= G238), S233 (≠ E239)

Sites not aligning to the query:

binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: 7

1jukA Indole-3-glycerolphosphate synthase from sulfolobus solfataricus in a trigonal crystal form (see paper)
38% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 1jukA

query
sites
1jukA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
|
K

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
|
E

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
|
N

N

S

R

R

D

D

L

L

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
|
E

S
|
S

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G
|
G

E
x
S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), K109 (= K116), E158 (= E164), N179 (= N185), E209 (= E215), S210 (= S216)
binding sulfate ion: K52 (= K56), G232 (= G238), S233 (≠ E239)

1igsA Indole-3-glycerolphosphate synthase from sulfolobus solfataricus at 2.0 a resolution (see paper)
38% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 1igsA

query
sites
1igsA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
|
K

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
|
E

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
|
N

N

S

R

R

D

D

L

L

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
|
E

S
|
S

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G
|
G

E
x
S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), K109 (= K116), E158 (= E164), N179 (= N185), E209 (= E215), S210 (= S216)
binding phosphate ion: K52 (= K56), S210 (= S216), G232 (= G238), S233 (≠ E239)

1a53A Complex of indole-3-glycerolphosphate synthase from sulfolobus solfataricus with indole-3-glycerolphosphate at 2.0 a resolution (see paper)
38% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 1a53A

query
sites
1a53A

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F
|
F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
|
K

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
|
E

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
|
N

N

S

R

R

D

D

L

L

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
|
E

S
|
S

S
x
G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G
|
G

E
x
S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), K109 (= K116), E158 (= E164), N179 (= N185), E209 (= E215), S210 (= S216)
binding indole-3-glycerol phosphate: E50 (= E54), K52 (= K56), F88 (= F95), K109 (= K116), E158 (= E164), N179 (= N185), E209 (= E215), S210 (= S216), G211 (≠ S217), G232 (= G238), S233 (≠ E239)

4iwwA Computational design of an unnatural amino acid metalloprotein with atomic level accuracy (see paper)
35% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 4iwwA

query
sites
4iwwA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

A

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
x
M

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I
x
A

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T
x
I

E
|
E

V

I

H

T

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I
|
I

N

S

R

Q

D

D

L
x
D

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S
x
A

E
x
D

S

S

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G

G

E

S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), M109 (≠ K116), I157 (≠ T163), I178 (= I184), A208 (≠ S214), D209 (≠ E215)
binding cobalt (ii) ion: A132 (≠ I139), E158 (= E164), D183 (≠ L189)

5k7jA Structure of designed zinc binding protein ze2 bound to zn2+ (see paper)
35% identity, 78% coverage: 46:249/262 of query aligns to 41:236/240 of 5k7jA

query
sites
5k7jA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

S

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
x
A

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
x
G

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
x
H

N

-

R

-

D

-

L

-

R

-

D

-

F

-

S

S

T
x
H

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
x
A

S
x
H

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G

G

E

S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), A109 (≠ K116), G158 (≠ E164), H179 (≠ N185), A202 (≠ E215), H203 (≠ S216)
binding zinc ion: H179 (≠ N185), H181 (≠ T194), H203 (≠ S216)

3uxdA Designed protein ke59 r1 7/10h with dichlorobenzotriazole (dbt) (see paper)
35% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 3uxdA

query
sites
3uxdA

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E

V

I

Y

K

K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

A

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

G

A

L

V

V

S

-

I

I

L

L

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

D

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
x
W

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L
x
G

L

L

I
|
I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

A

T

I

E
x
V

V

I

H

N

N

D

E

E

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

I

G

I

I

I

N

S

R

R

D

D

L

L

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E

A

S

S

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L
x
E

I

I

G

G

E

S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

binding 5,7-dichloro-1H-benzotriazole: W109 (≠ K116), G130 (≠ L137), I132 (= I139), V158 (≠ E164), E230 (≠ L236)

4ou1A Crystal structure of a computationally designed retro-aldolase covalently bound to folding probe 1 [(6-methoxynaphthalen-2-yl) (oxiran-2-yl)methanol] (see paper)
33% identity, 78% coverage: 46:249/262 of query aligns to 41:243/247 of 4ou1A

query
sites
4ou1A

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
|
E

I

Y

K
|
K

K

R

A

K

S
x
D

P
|
P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

A

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S

F

I

I

L

S

T

T

E

E

K

E

K

K

Y

Y

F
|
F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
x
Y

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

A

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

L

T

I

E
x
I

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G

G

I

I

N
x
A

N

A

R
|
R

D
|
D

L
x
W

R

E

D

T

F
x
G

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
x
K

S
x
E

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

L

I

I

G

G

E

S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: E50 (= E54), K52 (= K56), Y109 (≠ K116), I158 (≠ E164), A179 (≠ N185), K209 (≠ E215), E210 (≠ S216)
binding (1S,2S)-1-(6-methoxynaphthalen-2-yl)propane-1,2-diol: D55 (≠ S59), P56 (= P60), F88 (= F95), Y109 (≠ K116), R181 (= R187), D182 (= D188), W183 (≠ L189), G186 (≠ F192), K209 (≠ E215), E210 (≠ S216)

3nz1A Crystal structure of kemp elimination catalyst 1a53-2 complexed with transition state analog 5-nitro benzotriazole (see paper)
33% identity, 78% coverage: 46:249/262 of query aligns to 41:243/249 of 3nz1A

query
sites
3nz1A

K

K

E

R

N

N

I

I

S

T

-

A

I

I

A

A

E
x
A

I

Y

K
|
K

K

R

A

K

S

S

P

P

S

S

K

G

G

L

V

D

I

V

R

E

E

R

D

D

F

-

N

-

P

P

V

I

K

E

I

Y

G

S

Q

K

I

F

Y

M

E

E

N

R

I

Y

N

A

I

V

D

-

A

G

V

L

S
x
A

I

I

L

A

T

T

E

E

K

E

K

K

Y

Y

F

F

L

N

G

G

K

S

N

Y

E

E

Y

T

I

L

K

R

I

K

V

I

K

A

E

S

V

S

N

V

S

S

K

I

P

P

I

I

L

L

R

M

K
x
W

D

D

F

F

I

I

V

V

D

K

E

E

Y

S

Q

Q

L

I

Y

D

E

D

A

A

K

Y

L

N

I

L

G

G

A

A

D

D

A

T

V

V

L

A

L

L

I

I

A

V

A

K

V

I

L

L

K

T

D

E

K

R

-

E

L

L

E

E

C

S

F

L

Y

L

N

E

R

Y

T

A

L

R

E

S

L

Y

G

G

L

M

D

E

S

P

I

A

T

I

E
x
V

V

I

H

N

N

D

E

E

E

N

E

D

A

L

K

D

L

I

A

A

S

L

E

R

I

I

G

G

C

A

S

R

I

F

I

I

G
x
E

I

I

N
x
A

N

S

R

R

D

D

L

L

R

E

D

T

F

L

S

E

T

I

D

N

I

K

T

E

T

N

T

Q

K

R

R

K

L

L

M

I

K

S

Y

M

V

I

P

P

R

S

D

N

R

V

I

V

I

K

V

V

S

A

E
x
W

S
x
Q

S

G

I

I

K

S

T

E

P

R

E

N

D

E

I

I

L

E

Y

E

L

L

R

R

S

K

I

L

G

G

V

V

N

N

A

A

V

F

L

G

I

I

G

G

E

S

T

S

F

L

M

M

R

R

N

N

I

P

D

E

D

K

L

I

K

K

active site: A50 (≠ E54), K52 (= K56), W109 (≠ K116), V158 (≠ E164), A179 (≠ N185), W209 (≠ E215), Q210 (≠ S216)
binding 5-nitro-1H-benzotriazole: K52 (= K56), A80 (≠ S87), W109 (≠ K116), E177 (≠ G183), W209 (≠ E215), Q210 (≠ S216)

Query Sequence

>WP_010966437.1 NCBI__GCF_000008765.1:WP_010966437.1
MILDDIVRDKKLQLIEDKKALSLDDIKSKLNSLNLDKRNFKEALEKENISIIAEIKKASP
SKGVIREDFNPVKIGQIYENINIDAVSILTEKKYFLGKNEYIKIVKEVNSKPILRKDFIV
DEYQLYEAKLIGADAVLLIAAVLKDKLECFYNRTLELGLDSITEVHNEEEAKLASEIGCS
IIGINNRDLRDFSTDITTTKRLMKYVPRDRIIVSESSIKTPEDILYLRSIGVNAVLIGET
FMRNIDDLKGINEFLKKAKDNG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory