SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010966440.1 NCBI__GCF_000008765.1:WP_010966440.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
43% identity, 93% coverage: 27:467/475 of query aligns to 30:466/489 of O94582

query
sites
O94582

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S390 (= S392) modified: Phosphoserine
S392 (≠ A394) modified: Phosphoserine

Sites not aligning to the query:

488 modified: Phosphoserine

P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
40% identity, 91% coverage: 42:475/475 of query aligns to 30:460/470 of P28820

query
sites
P28820

K

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A283 (= A298) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.

7pi1DDD Aminodeoxychorismate synthase component 1
40% identity, 91% coverage: 42:475/475 of query aligns to 28:453/459 of 7pi1DDD

query
sites
7pi1DDD

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G

A

G
|
G

V

V

Y

I

D

D

S

S

I

V

P

P

E

K

N

H

E
|
E

Y

Y

E

K

E

E

S

S

M

F

N

K

K

K

S

A

Q

F

I

A

L

M

K

Q

E

R

V

A

I

L

binding magnesium ion: G428 (= G450), E438 (= E460)
binding tryptophan: L33 (= L47), E34 (= E48), S35 (= S49), G39 (= G57), Y41 (= Y59), P242 (= P264), Y243 (= Y265), M244 (≠ L266), Q406 (≠ D428), N408 (≠ C430)

A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
39% identity, 99% coverage: 3:471/475 of query aligns to 26:511/524 of A0QX93

query
sites
A0QX93

N

T

I

T

S

S

E

R

E

E

Q

D

F

F

N

R

D

A

N

L

K

A

K

T

L

E

K

H

K

R

A

V

F

V

Q

P

V

V

I

I

Y

R

E

K

E

V

N

L

G

A

D

D

E

S

I

E

T

T

P

P

I

L

S

S

L

A

F

Y

Y

R

N

K

L

L

E

A

G

A

K

N

-

R

-

P

N

G

K

T

F

F

L

L

E

E

S

S

N

A

L

E

F

N

S

G

E

R

S

S

K

W

G

S

R

R

Y

W

S

S

F

F

I

I

G

G

A

A

N

G

P

A

Y

P

A

S

I

A

I

L

K

T

G

V

F

R

S

D

N

N

N

A

T

A

E

A

I

W

I

L

R

G

N

T

G

A

Y

P

V

E

K

G

K

A

V

P

E

S

D

G

-

G

K

D

F

P

L

L

K

D

V

A

A

L

R

R

K

A

L

T

T

L

E

D

E

L

G

L

K

K

I

T

E

E

K

A

S

M

K

A

-

G

-

L

Y

P

P

P

F

L

C

S

G

S

G

G

G

L

V

V

G

G

Y

F

V

F

G

A

Y

Y

D

D

I

M

I

V

K

R

Q

R

Y

L

E

E

K

R

I

L

P

P

S

E

L

L

N

A

E

V

D

D

L

L

K

G

I

L

P

P

D

D

A

M

I

L

L

L

M

L

F

L

Y

A

K

T

K

D

I

I

I

A

C

A

Y

V

D

D

H

H

V

H

K

E

N

G

S

T

I

I

V

T

Y

L

I

I

Y

A

N

N

V

A

F

V

R

N

E

W

D

N

-

G

-

T

-

D

-

E

-

R

-

V

D

D

K

W

S

A

Y

Y

K

D

E

D

I

A

K

V

Q

A

E

R

L

L

F

D

E

V

L

M

S

T

E

K

I

A

S

L

K

G

K

Q

T

P

R

L

E

T

M

S

H

A

K

V

L

A

P

T

E

F

S

S

H

R

N

P

P

A

A

P

N

D

I

H

S

R

S

A

N

Q

F

R

T

T

K

M

D

E

E

E

F

Y

C

T

K

E

M

I

V

V

K

D

K

K

A

L

Q

V

E

G

Y

D

I

I

V

E

K

A

G

G

D

E

I

A

F

F

Q

Q

M

V

V

V

P

P

S

S

Q

Q

R

R

F

F

V

E

M

M

D

D

I

T

D

A

E

A

R

D

P

P

F

L

N

D

V

V

Y

Y

R

R

R

I

L

L

R

R

G

V

K

T

N

N

P

P

S

S

P

P

Y

Y

L

M

F

Y

Y

L

I

L

D

N

F

I

N

P

D

D

-

A

-

D

-

G

-

L

-

D

F

F

Q

S

V

I

T

V

G

G

S

S

P

P

E

E

S

A

L

L

V

V

C

T

V

V

F

K

K

D

D

G

R

R

V

A

I

T

T

T

N

H

P

P

I

I

A

A

G

G

T

T

R

R

P

W

R

R

G

G

K

A

N

T

E

E

Q

E

E

E

D

D

I

V

K

L

L

L

K

E

S
x
K

E

E

L

L

I

L

N

A

D

D

E

E

K

K

E

E

I

R

A

A

E

E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

G

R

R

N

N

D

D

I

L

G

G

K

R

I

V

S

C

E

R

F

P

G

G

T

T

V

V

T

R

V

V

D

D

K

D

F

Y

M

S

E

H

V

I

E

E

L

R

Y

Y

S

S

H

H

V

V

M

M

H

H

I

L

V

V

S

S

K

T

V

V

S

T

G

G

R

E

L

L

K

A

K

E

E

D

Y

K

D

T

C

A

F

L

E

D

A

A

L

V

K

T

S

A

C

C

L

F

P

P

A

A

G

G

T

T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

D

E

E

E

L

V

E

E

N

K

T

T

K

R

R

R

G

G

C

L

Y

Y

A

G

G

G

A

V

V

V

G

G

Y

Y

F

L

S

D

Y

F

D

A

G

G

N

N

M

A

D

D

T

F

C

A

I

I

A

A

I

I

R

R

T

T

L

A

V

L

I

M

K

R

E

N

G

G

K

T

A

A

Y

Y

A

V

Q

Q

A

A

G

G

V

V

Y

A

D

D

S

S

I

N

P

G

E

P

N

Y

E

E

Y

Y

E

T

E

E

S

A

M

A

N

N

K

K

S

A

Q

R

I

A

L

V

K355 (≠ S315) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
38% identity, 99% coverage: 3:471/475 of query aligns to 6:490/505 of 5cwaA

query
sites
5cwaA

N

T

I

T

S

S

E

R

E

E

Q

D

F

F

N

R

D

L

N

L

K

A

L

E

K

H

K

R

A

V

F

V

Q

P

V

V

I

T

Y

R

E

K

E

V

N

L

G

A

D

D

E

S

I

E

T

T

P

P

I

L

S

S

L

A

F

Y

Y

R

N

K

L

L

E

A

G

A

K

N

-

R

-

P

N

G

K

T

F

F

L

L

E

E

S

S

N

A

L

E

F

N

S

G

E

R

S

S

K

W

G

S

R

R

Y

W

S

S

F

F

I

I

G

G

A

A

N

G

-

A

P

P

Y

T

A

A

I

L

I

T

K

-

G

-

F

-

S

V

N

R

N

E

T

G

E

Q

I

A

I

V

R

W

N

L

G

G

Y

A

V

V

K

P

K

K

V

D

E

A

-

P

-

T

-

G

D

D

K

P

F

L

L

R

K

A

V

L

A

Q

R

V

K

T

L

L

T

E

E

L

G

A

K

T

I

A

E

D

K

P

S

G

K

L

Y

P

P

P

F

L

C

S

G

G

G

M

V

V

G

G

Y

F

V

F

G

A

Y

Y

D

D

I

M

I

V

K

R

Q

R

Y

L

E

E

K

R

I

L

P

P

S

E

L

R

N

A

E

V

D

D

L

L

K

C

I

L

P

P

D

D

A

M

I

L

L

L

M

L

F

L

Y

A

K

T

K

D

I

V

I

A

C

A

Y

V

D

D

H

H

V

H

K

E

N

G

S

T

I

I

V

T

Y

L

I

I

Y

A

N

N

V

A

F

V

R

N

E

W

D

N

-

G

-

T

-

D

-

E

-

R

-

V

D

D

K

W

S

A

Y

Y

K

D

E

D

I

A

K

V

Q

A

E

R

L

L

-

D

-

V

-

M

-

T

F

A

E

A

L

L

S

G

E

Q

I

P

S

L

K

P

K

S

T

T

R

V

E

A

M

T

H

F

K

S

L

R

P

P

E

E

S

P

H

R

N

H

P

R

A

A

N

Q

I

-

S

-

N

-

F

R

T

T

K

V

D

E

E

E

F

Y

C

G

K

A

M

I

V

V

K

E

K

Y

A

L

Q

V

E

D

Y

Q

I

I

V

A

K

A

G

G

D

E

I

A

F

F

Q
|
Q

M

V

V

V

P

P

S

S

Q

Q

R

R

F

F

V

E

M

M

D

D

I

T

D

D

E

V

R

D

P

P

F

I

N

D

V

V

Y

Y

R

R

R

I

L

L

R

R

G

V

K

T

N

N

P

P

S

S

P

P

Y

Y

L

M

F

Y

Y

L

I

L

D

Q

F

V

N

P

-

N

-

S

-

D

-

G

-

A

-

V

D

D

F

F

Q

S

V

I

T

V

G

G

S

S

P

P

E
|
E

S

A

L

L

V

V

C

T

V

V

F

H

K

E

D

G

R

W

V

A

I

T

T

T

N

H

P

P

I

I

A
|
A

G

G

T

T

R

R

P

W

R

R

G

G

K

R

N

T

E

D

Q

D

E

E

D

D

I

V

K

L

L

L

K

E

S

K

E

E

L

L

I

L

N

A

D

D

E

D

K

K

E

E

I

R

A

A

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

G

R

R

N

N

D

D

I

L

G

G

K

R

I

V

S

C

E

T

F

P

G

G

T

T

V

V

T

R

V

V

D

E

K

D

F

Y

M

S

E

H

V

I

E

E

L

R

Y

Y

S

S

H

H

V

V

M

M

H
|
H

I

L

V

V

S

S

K

T

V

V

S

T

G

G

R

K

L

L

K

G

K

E

E

G

Y

R

D

T

C

A

F

L

E

D

A

A

L

V

K

T

S

A

C

C

L

F

P

P

A

A

G

G

T
|
T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

D

E

E

E

L

V

E

E

N

K

T

T

K

R

R

R

G

G

C

L

Y
|
Y

A

G

G

G

A

V

V

V

G

G

Y

Y

F

L

S

D

Y

F

D

A

G

G

N

N

M

A

D

D

T

F

C

A

I

I

A

A

I
|
I

R
|
R

T

T

L

A

V

L

I

M

K

R

E

N

G

G

K

T

A

A

Y

Y

A

V

Q

Q

A
|
A

G
|
G

G

G

G
|
G

V

V

Y

A

D

D

S

S

I

N

P

G

E

S

N

Y

E

E

Y

Y

E

N

E
|
E

S

A

M

R

N

N

K
|
K

S

A

Q

R

I

A

L

V

active site: Q248 (= Q235), E301 (= E282), A317 (= A298), E345 (= E326), H382 (= H363), T409 (= T390), Y433 (= Y414), R453 (= R434), G469 (= G450), E482 (= E463), K486 (= K467)
binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y414), I452 (= I433), A466 (= A447), G467 (= G448), K486 (= K467)

P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 98% coverage: 9:475/475 of query aligns to 87:585/595 of P32068

query
sites
P32068

F

F

N

S

D

D

N

Q

K

L

K

T

L

P

K

V

K

L

A

A

F

Y

Q

R

V

C

I

L

Y

V

E

K

E

E

N

D

G

-

D

D

E

R

I

E

T

A

P

P

I

S

S

F

L

L

F

F

Y

E

N

S

L

V

E

E

G

P

K

G

N

S

K

Q

F

M

L

-

E

-

S

-

N

-

L

-

F

-

S

-

E

S

S

S

K

V

G

G

R

R

Y

Y

S

S

F

V

I

V

G

G

A

A

N

Q

P

P

Y

A

A

M

I

E

I

I

K

V

G

A

F

K

S

E

N

N

-

K

-

V

-

I

-

V

-

M

-

D

-

H

N

N

T

N

E

E

I

T

I

M

R

T

N

E

G

E

Y

F

V

V

K

-

V

-

E

E

D

D

K

P

F

-

L

M

K

E

V

I

A

P

R

R

K

K

L

I

T

S

E

E

E

K

G

W

K

N

-

P

-

D

-

P

-

Q

-

L

I

V

E

Q

K

D

S

L

K

P

Y

D

P

A

F

F

C

C

G

G

G

W

V

V

G

G

Y

F

V

F

G

S

Y

Y

D

D

I

T

I

V

K

R

Q

Y

Y

V

E

E

K

K

-

R

-

K

I

L

P

P

-

F

-

S

S

K

L

A

N

P

E

E

D

D

L

R

K

N

I

L

P

P

D

D

A

M

I

H

L

L

M

G

F

L

Y

Y

K

D

I

V

C

V

Y

F

D

D

H

H

V

V

K

E

N

K

S

K

I

-

V

A

Y

Y

I

V

Y

I

N

H

V

W

F

I

R

R

E

L

D

D

-

G

-

S

-

L

-

P

-

Y

D

E

K

K

S

A

Y

Y

K

S

E

N

I

G

K

M

Q

Q

E

H

L

L

F

E

E

N

L

L

-

V

-

A

-

K

-

L

-

H

-

D

-

I

-

E

-

P

-

K

-

L

-

A

S

G

E

N

I

V

S

N

K

L

K

Q

T

T

R

R

E

Q

M

F

H

-

K

-

L

-

P

-

E

-

S

-

H

-

N

G

P

P

A

S

N

L

I

D

S

N

S

S

N

N

F

V

T

T

K

C

D

E

E

E

F

Y

C

K

K

E

M

A

V

V

K

V

K

K

A

A

Q

K

E

E

Y

H

I

I

V

L

K

A

G

G

D

D

I

I

F

F

Q

Q

M

I

V

V

P

L

S

S

Q

Q

R

R

F

F

V

E

M

R

D

R

I

T

D

F

E

A

R
x
D

P

P

F

F

N

E

V

V

Y

Y

R

R

R

A

L

L

R

R

G

V

K

V

N

N

P

P

S

S

P

P

Y

Y

L

M

F

G

Y

Y

I

L

D

Q

F

A

N

R

D

G

F

C

Q

I

V

L

T

V

G

A

S

S

P

P

E

E

S

I

L

L

V

T

C

K

V

V

F

K

K

Q

D

N

R

K

V

I

I

V

T

N

N

R

P

P

I

L

A

A

G

G

T

T

R

S

P

K

R

R

G

G

K

K

N

N

E

E

Q

V

E

E

D

D

I

K

K

R

L

L

K

E

S

K

E

E

L

L

I

L

N

E

D

N

E

E

K

K

E

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I

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A

A

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E

H

H

S

I

M

M

L

L

L

V

D

D

L

L

A

G

R

R

N

N

D

D

I

V

G

G

K

K

I

V

S

T

E

K

F

Y

G

G

T

S

V

V

T

K

V

V

D

E

K

K

F

L

M

M

E

N

V

I

E

E

L

R

Y

Y

S

S

H

H

V

V

M

M

H

H

I

I

V

S

S

S

K

T

V

V

S

T

G

G

R

E

L

L

K

Q

K

D

E

G

Y

L

D

T

C

C

F

W

E

D

A

V

L

L

K

R

S

A

C

A

L

L

P

P

A

V

G

G

T

T

V

V

S

S

G

G

A

A

P

P

K

K

I

V

R

K

A

A

M

M

E

E

I

L

I

I

D

D

E

E

L

L

E

E

N

P

T

T

K

R

R

R

G

G

C

P

Y

Y

A

S

G

G

A

G

V

F

G

G

Y

G

F

V

S

S

Y

F

D

T

G

G

N

D

M

M

D

D

T

I

C

A

I

L

A

S

I

L

R

R

T

T

L

I

V

V

I

F

-

P

-

T

-

A

-

C

-

Q

-

Y

-

N

-

T

-

M

-

Y

-

S

-

Y

-

K

-

D

-

A

-

N

-

K

-

R

K

R

E

E

G

W

K

V

A

A

Y

Y

A

L

Q

Q

A

A

G

G

G

A

G

G

V

V

Y

A

D

D

S

S

I

D

P

P

E

Q

N

D

E

E

Y

H

E

C

E

E

S

C

M

Q

N

N

K

K

S

A

Q

A

I

G

L

L

K

A

E

R

V

A

I

I

D341 (≠ R249) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.

Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
37% identity, 98% coverage: 9:475/475 of query aligns to 71:567/577 of Q94GF1

query
sites
Q94GF1

F

F

N

S

D

D

N

H

K

L

K

T

L

P

K

V

K

L

A

A

F

Y

Q

R

V

C

I

L

Y

V

E

R

E

E

N

D

G

-

D

D

E

R

I

E

T

A

P

P

I

S

S

F

L

L

F

F

Y

E

N

S

L

V

E

E

G

Q

K

G

N

S

K

E

F

-

L

-

E

-

S

-

N

-

L

-

F

-

S

G

E

T

S

N

K

V

G

G

R

R

Y

Y

S

S

F

V

I

V

G

G

A

A

N

Q

P

P

Y

A

A

M

I

E

I

I

K

V

G

A

F

K

S

A

N

N

N

H

T

V

E

T

I

V

I

M

R

-

N

D

G

H

Y

K

V

M

K

K

K

S

V

R

E

R

D

E

K

Q

F

F

-

A

-

P

-

D

-

P

L

M

K

K

V

I

A

P

R

R

K

S

L

I

T

M

E

E

E

Q

G

W

K

N

-

P

-

Q

-

I

I

V

E

E

K

G

S

L

K

P

Y

D

P

A

F

F

C

C

G

G

G

W

V

V

G

G

Y

F

V

F

G

S

Y

Y

D

D

I

T

I

V

K

R

Q

Y

Y

V

E

E

-

T

-

K

K

K

I

L

P

P

S

F

L

S

N

N

-

A

-

P

E

E

D

D

L

R

K

N

I

L

P

P

D

D

A

I

I

H

L

L

M

G

F

L

Y

Y

K

N

K

D

I

I

I

V

C

V

Y

F

D

D

H

H

V

V

K

E

N

K

S

K

I

T

V

H

Y

V

I

I

Y

H

N

W

V

V

F

-

R

R

E

V

D

D

-

C

-

H

-

E

-

S

-

V

D

D

K

E

S

A

Y

Y

K

E

E

D

I

G

K

K

Q

N

E

Q

L

L

F

E

E

A

L

L

-

L

-

S

-

R

-

L

-

H

-

S

S

V

E

N

I

V

S

P

K

T

K

L

T

T

R

A

E

G

M

S

H

V

K

K

L

L

P

N

E

V

S

G

H

Q

-

F

N

G

P

S

A

A

N

L

I

Q

S

K

S

S

N

S

F

M

T

S

K

R

D

E

E

D

F

Y

C

K

K

K

M

A

V

V

K

V

K

Q

A

A

Q

K

E

E

Y

H

I

I

V

L

K

A

G

G

D

D

I

I

F

F

Q

Q

M

V

V

V

P

L

S

S

Q

Q

R

R

F

F

V

E

M

R

D

R

I

T

D

F

E

A

R
x
D

P

P

F

F

N

E

V

V

Y

Y

R

R

R

A

L

L

R

R

G

I

K

V

N

N

P

P

S

S

P

P

Y

Y

L

M

F

A

Y

Y

I

L

D

Q

F

A

N

R

D

G

F

C

Q

I

V

L

T

V

G

A

S

S

P

P

E

E

S

I

L

L

V

T

C

R

V

V

F

E

K

K

D

R

R

T

V

I

I

V

T

N

N

R

P

P

I

L

A

A

G

G

T

T

R

I

P

R

R

R

G

G

K

K

N

S

E

K

Q

A

E

E

D

D

I

K

K

V

L

L

K

E

S

Q

E

L

L

L

I

L

N

S

D

D

E

G

K

K

E

Q

I

C

A

A

E

E

H

H

S

I

M

M

L

L

L

V

D

D

L

L

A

G

R

R

N

N

D

D

I

V

G

G

K

K

I

V

S

S

E

K

F

P

G

G

T

S

V

V

T

K

V

V

D

E

K

K

F

L

M

M

E

N

V

V

E

E

L

R

Y

Y

S

S

H

H

V

V

M

M

H

H

I

I

V

S

S

S

K

T

V

V

S

T

G

G

R

E

L

L

K

R

K

D

E

D

Y

L

D

T

C

C

F

W

E

D

A

A

L

L

K

R

S

A

C

A

L

L

P

P

A

V

G

G

T

T

V

V

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

D

D

E

Q

L

M

E

E

N

G

T

K

K

M

R

R

G

G

C

P

Y

Y

A

S

G

G

A

G

V

F

G

G

Y

G

F

V

S

S

Y

F

D

R

G

G

N

D

M

M

D

D

T

I

C

A

I

L

A

A

I

L

R

R

T

T

L

I

V

V

I

F

K

P

E

T

G

G

K

S

-

R

-

F

-

D

-

T

-

M

-

Y

-

S

-

Y

-

T

-

D

-

K

-

N

-

A

-

R

-

Q

-

E

-

W

-

V

A

A

Y

H

A

L

Q

Q

A

A

G

G

G

A

G

G

V

I

V

V

Y

A

D

D

S

S

I

K

P

P

E

D

N

D

E

E

Y

H

E

Q

E

E

S

C

M

L

N

N

K

K

S

A

Q

A

I

G

L

L

K

A

E

R

V

A

I

I

D323 (≠ R249) mutation to N: Insensitive to feedback inhibition by tryptophan.

7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
38% identity, 99% coverage: 3:471/475 of query aligns to 6:486/499 of 7bvdA

query
sites
7bvdA

N

T

I

T

S

S

E

R

E

E

Q

D

F

F

N

R

D

A

N

L

K

A

K

T

L

E

K

H

K

R

A

V

F

V

Q

P

V

V

I

I

Y

R

E

K

E

V

N

L

G

A

D

D

E

S

I

E

T

T

P

P

I

L

S

S

L

A

F

Y

Y

R

N

K

L

L

E

A

G

A

K

N

-

R

-

P

N

G

K

T

F

F

L

L

E

E

S

S

N

A

L

-

F

E

S

N

E

R

S

S

K

W

G

S

R

R

Y

W

S

S

F

F

I

I

G

G

A

A

N

G

P

A

Y

P

A

S

I

A

I

L

K

T

G

V

F

R

S

D

N

N

N

A

T

A

E

A

I

W

I

L

R

G

N

T

G

A

Y

P

V

E

K

G

K

A

V

P

E
x
S

D
x
G

-

G

K

D

F

P

L

L

K

D

V
x
A

A

L

R

R

K

A

L

T

T

L

E

D

E

L

G

L

K

K

I

T

E

E

K

A

S

M

K

A

-

G

-

L

Y

P

P

P

F

L

C

S

G

S

G

G

G

L

V

V

G

G

Y

F

V

F

G

A

Y

Y

D

D

I

M

I

V

K

R

Q

R

Y

L

E

E

K

R

I

L

P

P

S

E

L

L

N

A

E

V

D

D

L

L

K

G

I

L

P

P

D

D

A

M

I

L

L

L

M

L

F

L

Y

A

K

T

K

D

I

I

I

A

C

A

Y

V

D

D

H

H

V

H

K

E

N

G

S

T

I

I

V

T

Y

L

I

I

Y

A

N

N

V

A

F

V

R

N

E

W

D

N

-

G

-

T

-

D

-

E

-

R

-

V

D

D

K

W

S

A

Y

Y

K

D

E

D

I

A

K

V

Q

A

E

R

L

L

F

D

E

V

L

M

S

T

E

K

I

A

S

L

K

G

K

Q

T

P

R

L

E

T

M

S

H

A

K

V

L

A

P

T

E

F

S

S

H

R

N

P

P

A

A

P

N

D

I

H

S

R

S

A

N

Q

F

R

T

T

K

M

D

E

E

E

F

Y

C

T

K

E

M

I

V

V

K

D

K

K

A

L

Q

V

E

G

Y

D

I

I

V

E

K

A

G

G

D

E

I

A

F

F

Q
|
Q

M

V

V

V

P

P

S

S

Q

Q

R

R

F

F

V

E

M

M

D

D

I

T

D

A

E

A

R

D

P

P

F

L

N

D

V

V

Y

Y

R

R

R

I

L

L

R

R

G

V

K

T

N

N

P

P

S

S

P

P

Y

Y

L

M

F

Y

Y

L

I

L

D

N

F

I

N

P

D

D

-

A

-

D

-

G

-

L

-

D

F

F

Q

S

V

I

T

V

G

G

S

S

P

P

E
|
E

S

A

L

L

V

V

C

T

V

V

F

K

K

D

D

G

R

R

V

A

I

T

T

T

N

H

P

P

I

I

A
|
A

G

G

T

T

R

R

P

W

R

R

G

-

K

-

N

-

E

-

Q

E

E

E

D

D

I

V

K

L

L

L

K

E

S

K

E

E

L

L

I

L

N

A

D

D

E

E

K

K

E

E

I

R

A

A

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

G

R

R

N

N

D

D

I

L

G

G

K

R

I

V

S

C

E

R

F

P

G

G

T

T

V

V

T

R

V

V

D

D

K

D

F

Y

M

S

E

H

V

I

E

E

L

R

Y

Y

S

S

H

H

V

V

M

M

H
|
H

I

L

V

V

S

S

K

T

V

V

S

T

G

G

R

E

L

L

K

A

K

E

E

D

Y

K

D

T

C

A

F

L

E

D

A

A

L

V

K

T

S

A

C

C

L

F

P

P

A

A

G

G

T
|
T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

D

E

E

E

L

V

E

E

N

K

T

T

K

R

R

R

G

G

C

L

Y
|
Y

A

G

G

G

A

V

V

V

G

G

Y

Y

F

L

S

D

Y

F

D

A

G

G

N

N

M

A

D

D

T

F

C

A

I

I

A

A

I

I

R
|
R

T

T

L

A

V

L

I

M

K

R

E

N

G

G

K

T

A

A

Y

Y

A

V

Q

Q

A

A

G

G

G
|
G

V

V

Y

A

D

D

S

S

I

N

P

G

E

P

N

Y

E

E

Y

Y

E

T

E
|
E

S

A

M

A

N

N

K
|
K

S

A

Q

R

I

A

L

V

active site: Q248 (= Q235), E301 (= E282), A317 (= A298), E341 (= E326), H378 (= H363), T405 (= T390), Y429 (= Y414), R449 (= R434), G465 (= G450), E478 (= E463), K482 (= K467)
binding pyruvic acid: S93 (≠ E89), G94 (≠ D90), A100 (≠ V95)

1i7sA Anthranilate synthase from serratia marcescens in complex with its end product inhibitor l-tryptophan (see paper)
37% identity, 75% coverage: 118:475/475 of query aligns to 139:501/511 of 1i7sA

query
sites
1i7sA

G

G

Y

L

V

F

G

A

Y

Y

D

D

I

L

I

V

K

A

Q

G

Y

F

E

E

K

N

I

L

P

P

S

A

L

L

N

R

E

Q

D

D

D

Q

L

-

K

R

I

C

P

P

D

D

A

F

I

C

L

F

M

Y

F

L

Y

A

K

E

K

T

I

L

C

V

Y

L

D

D

H

H

V

Q

K

R

N

G

S

S

I

A

V

R

Y

L

I

Q

Y

A

N

S

V

V

F

F

R

S

E

E

D

Q

D

A

K

S

S

E

Y

A

K

Q

E

R

I

L

K

Q

Q

H

E

R

L

L

F

E

E

Q

L

L

S

Q

E

A

I

E

S

L

K

Q

T

P

R

P

E

Q

M

P

H

I

K

P

L

H

P

Q

E

K

S

L

H

E

N

N

P

-

A

M

N

Q

I

L

S

S

S

C

N

N

F

Q

T

S

K

D

D

E

E

E

F

Y

C

G

K

A

M

V

V

V

K

S

K

E

A

L

Q

Q

E

E

Y

A

I

I

V

R

K

Q

G

G

D

E

I

I

F

F

Q
|
Q

M

V

V

V

P

P

S

S

Q

R

R

R

F

F

V

S

M

L

D

P

I

C

D

P

E

A

R

-

P

P

F

L

N

G

V

P

Y

Y

R

Q

R

T

L

L

R

K

G

D

K

N

N

N

P

P

S

S

P
|
P

Y
|
Y

L
x
M

F

F

Y

F

I

M

D

Q

F

D

N

D

D

D

F

F

Q

T

V

L

T

F

G

G

S

A

S

S

P

P

E
|
E

S

S

L

A

V

L

C

K

-

Y

-

D

V

A

F

G

K

N

D

R

R

Q

V

I

I

E

T

I

N

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

K

R

N

R

E

A

Q

D

E

G

-

S

-

L

-

D

-

L

-

D

-

L

D

D

I

S

K

R

L

I

K

E

S

L

E

E

L

M

I

R

N

T

D

D

E

H

K

K

E

E

I

L

A

A

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

A

R

R

N

N

D

D

I

L

G

A

K

R

I

I

S

C

E

Q

F

A

G

G

T

S

V

R

T

Y

V

V

D

A

K

D

F

L

M

T

E

K

V

V

E

D

L

R

Y

Y

S

S

H

F

V

V

M

M

H
|
H

I

L

V

V

S

S

K

R

V

V

S

V

G

G

R

T

L

L

K

R

K

A

E

D

Y

L

D

D

C

V

F

L

E

H

A

A

L

Y

K

Q

S

A

C

C

L

M

P

N

A

M

G

G

T
|
T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

D

A

E

A

L

L

E

R

N

S

T

T

K

R

R

R

G

G

C

S

Y
|
Y

A

G

G

G

A

R

V
|
V

G
|
G

Y

Y

F

F

S

T

Y

A

D

V

G

R

N

N

M

L

D
|
D

T

T

C
|
C

I

I

A

V

I

I

R
|
R

T

S

L

A

V

Y

I

V

K

E

E

D

G

G

K

H

A

R

Y

T

A

V

Q

Q

A

A

G

G

G

A

G
|
G

V

V

Y

Q

D

D

S

S

I

I

P

P

E

E

N

R

E

E

Y

A

E

D

E
|
E

S

T

M

R

N

N

K
|
K

S

A

Q

R

I

A

L

V

K

L

E

R

V

A

I

I

active site: Q254 (= Q235), E300 (= E282), A318 (= A298), E352 (= E326), H389 (= H363), T416 (= T390), Y440 (= Y414), R460 (= R434), G476 (= G450), E489 (= E463), K493 (= K467)
binding tryptophan: P282 (= P264), Y283 (= Y265), M284 (≠ L266), V444 (= V418), G445 (= G419), D454 (= D428), C456 (= C430)

Sites not aligning to the query:

binding tryptophan: 35, 36, 37

1i7qA Anthranilate synthase from s. Marcescens (see paper)
37% identity, 75% coverage: 118:475/475 of query aligns to 145:507/517 of 1i7qA

query
sites
1i7qA

G

G

Y

L

V

F

G

A

Y

Y

D

D

I

L

I

V

K

A

Q

G

Y

F

E

E

K

N

I

L

P

P

S

A

L

L

N

R

E

Q

D

D

D

Q

L

-

K

R

I

C

P

P

D

D

A

F

I

C

L

F

M

Y

F

L

Y

A

K

E

K

T

I

L

C

V

Y

L

D

D

H

H

V

Q

K

R

N

G

S

S

I

A

V

R

Y

L

I

Q

Y

A

N

S

V

V

F

F

R

S

E

E

D

Q

D

A

K

S

S

E

Y

A

K

Q

E

R

I

L

K

Q

Q

H

E

R

L

L

F

E

E

Q

L

L

S

Q

E

A

I

E

S

L

K

Q

T

P

R

P

E

Q

M

P

H

I

K

P

L

H

P

Q

E

K

S

L

H

E

N

N

P

-

A

M

N

Q

I

L

S

S

S

C

N

N

F

Q

T

S

K

D

D

E

E

E

F

Y

C

G

K

A

M

V

V

V

K

S

K

E

A

L

Q

Q

E

E

Y

A

I

I

V

R

K

Q

G

G

D

E

I

I

F

F

Q
|
Q

M

V

V

V

P

P

S

S

Q

R

R

R

F

F

V

S

M

L

D

P

I

C

D

P

E

A

R

-

P

P

F

L

N

G

V

P

Y

Y

R

Q

R

T

L

L

R

K

G

D

K

N

N

N

P

P

S

S

P

P

Y

Y

L

M

F

F

Y

F

I

M

D

Q

F

D

N

D

D

D

F

F

Q

T

V

L

T

F

G

G

S

A

S

S

P

P

E
|
E

S

S

L

A

V

L

C

K

-

Y

-

D

V

A

F

G

K

N

D

R

R

Q

V

I

I

E

T

I

N

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

K

R

N

R

E

A

Q

D

E

G

-

S

-

L

-

D

-

L

-

D

-

L

D

D

I

S

K

R

L

I

K

E

S

L

E

E

L

M

I

R

N

T

D

D

E

H

K

K

E

E

I

L

A

A

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

A

R

R

N

N

D

D

I

L

G

A

K

R

I

I

S

C

E

Q

F

A

G

G

T

S

V

R

T

Y

V

V

D

A

K

D

F

L

M

T

E

K

V

V

E

D

L

R

Y

Y

S

S

H

F

V

V

M

M

H
|
H

I

L

V

V

S

S

K

R

V

V

S

V

G

G

R

T

L

L

K

R

K

A

E

D

Y

L

D

D

C

V

F

L

E

H

A

A

L

Y

K

Q

S

A

C

C

L

M

P

N

A

M

G

G

T
|
T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

D

A

E

A

L

L

E

R

N

S

T

T

K

R

R

R

G

G

C

S

Y
|
Y

A

G

G

G

A

R

V

V

G

G

Y

Y

F

F

S

T

Y

A

D

V

G

R

N

N

M

L

D

D

T

T

C

C

I

I

A

V

I
|
I

R
|
R

T

S

L

A

V

Y

I

V

K

E

E

D

G

G

K

H

A

R

Y

T

A

V

Q

Q

A
|
A

G
|
G

G

A

G
|
G

V

V

Y

Q

D

D

S

S

I

I

P

P

E

E

N

R

E

E

Y

A

E

D

E
|
E

S

T

M

R

N

N

K
|
K

S

A

Q

R

I

A

L

V

K

L

E

R

V

A

I

I

active site: Q260 (= Q235), E306 (= E282), A324 (= A298), E358 (= E326), H395 (= H363), T422 (= T390), Y446 (= Y414), R466 (= R434), G482 (= G450), E495 (= E463), K499 (= K467)
binding magnesium ion: E358 (= E326), E495 (= E463)
binding pyruvic acid: Y446 (= Y414), I465 (= I433), R466 (= R434), A479 (= A447), G480 (= G448), K499 (= K467)

P00898 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
37% identity, 75% coverage: 118:475/475 of query aligns to 148:510/520 of P00898

query
sites
P00898

G

G

Y

L

V

F

G

A

Y

Y

D

D

I

L

I

V

K

A

Q

G

Y

F

E

E

K

A

I

L

P

P

S

H

L

L

N

E

E

A

D

G

D

N

L

-

K

N

I

C

P

P

D

D

A

Y

I
x
C

L

F

M

Y

F

L

Y

A

K

E

K

T

I

L

I

M

C

V

Y

I

D

D

H

H

V

Q

K

K

N

K

S

S

I

T

V

R

Y

I

I

Q

Y

A

N

S

V

L

F

F

R

T

E

A

D

S

D

D

K

R

S

E

Y

K

K

Q

E

R

I

L

K

N

Q

A

E

R

L

L

F

A

E

Y

L

L

S

S

E

Q

I

-

S

-

K

Q

K

L

T

T

R

Q

E

P

M

A

H

P

K

P

L

L

P

P

E

V

S

T

H

P

N

V

P

P

-

D

A

M

N

R

I

C

S

E

S

C

N

N

F

Q

T

S

K

D

D

D

E

A

F

F

C

G

K

A

M

V

V

V

K

R

K

Q

A

L

Q

Q

E

K

Y

A

I

I

V

R

K

A

G

G

D

E

I

I

F

F

Q

Q

M

V

V

V

P

P

S

S

Q

R

R

R

F

F

V

S

M

L

D

P

I

C

D

P

E

S

R

-

P

P

F

L

N

A

V

A

Y

Y

R

Y

R

V

L

L

R

K

G

K

K

S

N
|
N

P
|
P

S

S

P

P

Y

Y

L
x
M

F
|
F

Y

F

I

M

D

Q

F

D

N

N

D

D

F

F

Q

T

V

L

T

F

G
|
G

S

A

S

S

P

P

E

E

S

S

L

S

V

L

C

K

-

Y

-

D

V

A

F

A

K

S

D

R

R

Q

V

I

I

E

T

I

N

Y

P

P

I

I

A

A

G

G

T

T

R

R

P

P

R

R

G

G

K

R

-

R

-

A

-

D

-

G

-

T

-

L

N

D

E

R

Q

D

E

L

D

D

I

S

K

R

L

I

K

E

S

L

E

D

L

M

I

R

N

T

D

D

E

H

K

K

E

E

I

L

A

S

E

E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

A

R

R

N

N

D

D

I

L

G

A

K

R

I

I

S

C

E

T

F

P

G

G

T

S

V

R

T

Y

V

V

D

A

K

D

F

L

M

T

E

K

V

V

E

D

L

R

Y

Y

S

S

H

Y

V

V

M

M

H

H

I

L

V

V

S

S

K
x
R

V

V

S

V

G

G

R

E

L

L

K

R

K

H

E

D

Y

L

D

D

C

A

F

L

E

H

A

A

L

Y

K

R

S

A

C

C

L

M

P

N

A

M

G

G

T

T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

D

A

E

D

L

A

E

E

N

G

T

Q

K

R

R

R

G

G

C

S

Y

Y

A

G

G

G

A

A

V

V

G

G

Y

Y

F

F

S

T

Y

A

D

H

G
|
G

N

D

M

L

D

D

T

T

C
|
C

I

I

A

V

I

I

R

R

T

S

L

A

V

L

I

V

K

E

E

N

G

G

K

I

A

A

Y

T

A

V

Q

Q

A

A

G

G

G

A

G

G

V

I

V

V

Y

L

D

D

S

S

I

V

P

P

E

Q

N

S

E

E

Y

A

E

D

E

E

S

T

M

R

N

N

K

K

S

A

Q

R

I

A

L

V

K

L

E

R

V

A

I

I

C174 (≠ I145) mutation to Y: Almost no change in feedback control by tryptophan.
N288 (= N261) mutation to D: Decrease in feedback control by tryptophan.
P289 (= P262) mutation to L: Decrease in feedback control by tryptophan.
M293 (≠ L266) mutation to T: Complete loss of feedback control by tryptophan.
F294 (= F267) mutation to L: Decrease in feedback control by tryptophan.
G305 (= G278) mutation to S: Decrease in feedback control by tryptophan.
R402 (≠ K367) mutation to W: Almost no change in feedback control by tryptophan.
G460 (= G425) mutation to D: Almost no change in feedback control by tryptophan.
C465 (= C430) mutation to Y: Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.

Sites not aligning to the query:

39 E→K: Complete loss of feedback control by tryptophan.
40 binding L-tryptophan; S→F: Complete loss of feedback control by tryptophan.
41 A→V: Decrease in feedback control by tryptophan.
50 binding L-tryptophan
128 R→H: Almost no change in feedback control by tryptophan.
515 H→Y: Almost no change in feedback control by tryptophan.

1i1qA Structure of the cooperative allosteric anthranilate synthase from salmonella typhimurium (see paper)
37% identity, 75% coverage: 118:475/475 of query aligns to 144:506/512 of 1i1qA

query
sites
1i1qA

G

G

Y

L

V

F

G

A

Y

Y

D

D

I

L

I

V

K

A

Q

G

Y

F

E

E

K

A

I

L

P

P

S

H

L

L

N

E

E

A

D

G

D

N

L

-

K

N

I

C

P

P

D

D

A

Y

I

C

L

F

M

Y

F

L

Y

A

K

E

K

T

I

L

I

M

C

V

Y

I

D

D

H

H

V

Q

K

K

N

K

S

S

I

T

V

R

Y

I

I

Q

Y

A

N

S

V

L

F

F

R

T

E

A

D

S

D

D

K

R

S

E

Y

K

K

Q

E

R

I

L

K

N

Q

A

E

R

L

L

F

A

E

Y

L

L

S

S

E

Q

I

-

S

-

K

Q

K

L

T

T

R

Q

E

P

M

A

H

P

K

P

L

L

P

P

E

V

S

T

H

P

N

V

P

P

-

D

A

M

N

R

I

C

S

E

S

C

N

N

F

Q

T

S

K

D

D

D

E

A

F

F

C

G

K

A

M

V

V

V

K

R

K

Q

A

L

Q

Q

E

K

Y

A

I

I

V

R

K

A

G

G

D

E

I

I

F

F

Q
|
Q

M

V

V

V

P

P

S

S

Q

R

R

R

F

F

V

S

M

L

D

P

I

C

D

P

E

S

R

-

P

P

F

L

N

A

V

A

Y

Y

R

Y

R

V

L

L

R

K

G

K

K

S

N

N

P

P

S

S

P
|
P

Y
|
Y

L
x
M

F

F

Y

F

I

M

D

Q

F

D

N

N

D

D

F

F

Q

T

V

L

T

F

G

G

S

A

S

S

P

P

E
|
E

S

S

L

S

V

L

C

K

-

Y

-

D

V

A

F

A

K

S

D

R

R

Q

V

I

I

E

T

I

N

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

K

R

-

R

-

A

-

D

-

G

-

T

-

L

N

D

E

R

Q

D

E

L

D

D

I

S

K

R

L

I

K

E

S

L

E

D

L

M

I

R

N

T

D

D

E

H

K

K

E

E

I

L

A

S

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

A

R

R

N

N

D

D

I

L

G

A

K

R

I

I

S

C

E

T

F

P

G

G

T

S

V

R

T

Y

V

V

D

A

K

D

F

L

M

T

E

K

V

V

E

D

L

R

Y

Y

S

S

H

Y

V

V

M

M

H
|
H

I

L

V

V

S

S

K

R

V

V

S

V

G

G

R

E

L

L

K

R

K

H

E

D

Y

L

D

D

C

A

F

L

E

H

A

A

L

Y

K

R

S

A

C

C

L

M

P

N

A

M

G

G

T
|
T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

D

A

E

D

L

A

E

E

N

G

T

Q

K

R

R

R

G

G

C

S

Y
|
Y

A

G

G

G

A

A

V

V

G
|
G

Y

Y

F

F

S

T

Y

A

D

H

G

G

N

D

M

L

D

D

T

T

C
|
C

I

I

A

V

I

I

R
|
R

T

S

L

A

V

L

I

V

K

E

E

N

G

G

K

I

A

A

Y

T

A

V

Q

Q

A

A

G

G

G

A

G
|
G

V

I

V

V

Y

L

D

D

S

S

I

V

P

P

E

Q

N

S

E

E

Y

A

E

D

E
|
E

S

T

M

R

N

N

K
|
K

S

A

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R

I

A

L

V

K

L

E

R

V

A

I

I

active site: Q259 (= Q235), E305 (= E282), A323 (= A298), E357 (= E326), H394 (= H363), T421 (= T390), Y445 (= Y414), R465 (= R434), G481 (= G450), E494 (= E463), K498 (= K467)
binding tryptophan: P287 (= P264), Y288 (= Y265), M289 (≠ L266), G450 (= G419), C461 (= C430)

Sites not aligning to the query:

binding tryptophan: 34, 35, 36, 46

P00897 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Serratia marcescens (see paper)
36% identity, 75% coverage: 118:475/475 of query aligns to 147:509/519 of P00897

query
sites
P00897

G

G

Y

L

V

F

G

A

Y

Y

D

D

I

L

I

V

K

A

Q

G

Y

F

E

E

K

N

I

L

P

P

S

A

L

V

N

R

E

Q

D

D

D

Q

L

-

K

R

I

C

P

P

D

D

A

F

I

C

L

F

M

Y

F

L

Y

A

K

E

K

T

I

L

C

V

Y

L

D

D

H

H

V

Q

K

R

N

G

S

S

I

A

V

R

Y

L

I

Q

Y

A

N

S

V

V

F

F

R

S

E

E

D

Q

D

A

K

S

S

E

Y

A

K

Q

E

R

I

L

K

Q

Q

H

E

R

L

L

F

E

E

Q

L

L

S

Q

E

A

I

E

S

L

K

Q

T

P

R

P

E

Q

M

P

H

I

K

P

L

H

P

Q

E

K

S

L

H

E

N

N

P

-

A

M

N

Q

I

L

S

S

S

C

N

N

F

Q

T

S

K

D

D

E

E

E

F

Y

C

G

K

A

M

V

V

V

K

S

K

E

A

L

Q

Q

E

E

Y

A

I

I

V

R

K

Q

G

G

D

E

I

I

F

F

Q

Q

M

V

V

V

P

P

S

S

Q

R

R

R

F

F

V

S

M

L

D

P

I

C

D

P

E

A

R

-

P

P

F

L

N

G

V

P

Y

Y

R

Q

R

T

L

L

R

K

G

D

K

N

N

N

P

P

S

S

P
|
P

Y
|
Y

L
x
M

F

F

Y

F

I

M

D

Q

F

D

N

D

D

D

F

F

Q

T

V

L

T

F

G

G

S

A

S

S

P

P

E

E

S

S

L

A

V

L

C

K

-

Y

-

D

V

A

F

G

K

N

D

R

R

Q

V

I

I

E

T

I

N

Y

P

P

I

I

A

A

G

G

T

T

R

R

P

P

R

R

G

G

K

R

N

R

E

A

Q

D

E

G

-

S

-

L

-

D

-

L

-

D

-

L

D

D

I

S

K

R

L

I

K

E

S

L

E

E

L

M

I

R

N

T

D

D

E

H

K

K

E

E

I

L

A

A

E
|
E

H

H

S

L

M

M

L

L

L

V

D

D

L

L

A

A

R

R

N

N

D

D

I

L

G

A

K

R

I

I

S

C

E

Q

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A

G

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S

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R

T

Y

V

V

D

A

K

D

F

L

M

T

E

K

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V

E

D

L

R

Y

Y

S

S

H

F

V

V

M

M

H

H

I

L

V

V

S

S

K

R

V

V

S

V

G

G

R

T

L

L

K

R

K

A

E

D

Y

L

D

D

C

V

F

L

E

H

A

A

L

Y

K

Q

S

A

C

C

L

M

P

N

A

M

G

G

T

T

V

L

S

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

D

A

E

A

L

L

E

R

N

S

T

T

K

R

R

R

G

G

C

S

Y

Y

A

G

G

G

A

R

V

V

G

G

Y

Y

F

F

S

T

Y

A

D

H

G

R

N

H

M

L

D

D

T

T

C

C

I

I

A

V

I

I

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R

T

S

L

A

V

Y

I

V

K

E

E

D

G

G

K

H

A

R

Y

T

A

V

Q

Q

A

A

G

G

G

A

G

G

V

V

Y

Q

D

D

S

S

I

I

P

R

E

R

N

R

E

E

Y

A

E

D

E
|
E

S

T

M

R

N

N

K

K

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A

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R

I

A

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V

K

L

E

R

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A

I

I

PYM 290:292 (≠ PYL 264:266) binding L-tryptophan
E360 (= E326) binding Mg(2+)
E497 (= E463) binding Mg(2+)

Sites not aligning to the query:

39 binding L-tryptophan

P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
31% identity, 91% coverage: 45:474/475 of query aligns to 32:451/453 of P05041

query
sites
P05041

F

M

L

L

E

H

S
|
S

N

G

L

Y

F

A

S

D

E

H

S

P

K

Y

G

S

R

R

Y

F

S

D

F

I

I

V

G

V

A

A

N

E

P

P

Y

I

A

C

I

T

I

L

K

T

G

T

F

F

S

G

N

K

N

E

T

T

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I

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S

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E

N

S

G

E

Y

K

V

R

K

T

V

T

E

T

D

D

K

D

F

P

L

L

K

Q

V

V

A

L

R

Q

K

Q

L

V

T

L

E

D

E

R

G

A

K

D

I

I

E

R

K

P

S

T

K

H

-

N

-

E

-

D

Y

L

P

P

F

F

C

Q

G

G

G

A

V

L

G

G

Y

L

V

F

G

G

Y

Y

D

D

I

L

I

G

K

R

Q

R

Y

F

E

E

K

S

I

L

P

P

S

E

L

I

N

A

E

E

D

Q

D

D

L

I

K

V

I

L

P

P

D

D

A

M

I

A

L

V

M

G

F

I

Y

Y

K

D

K

W

I

A

I

L

C

I

Y

V

D

D

H

H

V

Q

K

R

N

H

S

T

I

V

V

S

Y

L

I

L

-

S

-

H

-

N

-

D

Y

V

N

N

V

A

F

R

R

R

E

A

D

W

D

L

K

E

S

S

Y

Q

K

Q

E

F

I

S

K

P

Q

Q

E

E

L

D

F

F

E

T

L

L

S

T

E

-

I

-

S

-

K

-

T

-

R

-

E

-

M

-

H

-

K

-

L

-

P

-

E

-

S

-

H

-

N

-

P

-

A

S

N

D

I

W

S

Q

S

S

N

N

F

M

T

T

K

R

D

E

E

Q

F

Y

C

G

K

E

M

K

V

F

K

R

K

Q

A

V

Q

Q

E

E

Y

Y

I

L

V

H

K

S

G

G

D

D

I

C

F

Y

Q

Q

M

V

V

N

P

L

S

A

Q

Q

R

R

F

F

V

H

M

A

D

T

I

Y

D

S

E

G

R

D

P

E

F

W

N

Q

V

A

Y

F

R

L

R

Q

L

L

R

N

G

Q

K

A

N

N

P

R

S

A

P

P

Y

F

L

S

F

A

Y

F

I

L

D

R

F

L

N

E

D

Q

F

G

Q

A

V

I

T

L

G

S

S

L

S

S

P

P

E
|
E

S

R

L

F

V

I

C

L

V

C

F

D

K

N

D

S

R

E

V

I

I

Q

T

T

N

R

P

P

I

I

A
x
K

G
|
G

T

T

R

L

P

P

R

R

G

L

K

P

N

D

E

P

Q

Q

E

E

D

D

I

S

K

K

L

Q

K

A

S

V

E

K

L

L

I

A

N

N

D

S

E

A

K

K

E

D

I

R

A

A

E

E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

M

R
|
R

N

N

D

D

I

I

G

G

K
x
R

I

V

S

A

E

V

F

A

G

G

T
x
S

V

V

T

K

V

V

D

P

K

E

F

L

M

F

E

V

V

V

E

E

L

P

Y

F

S

P

H

A

V

V

M

H

H
|
H

I

L

V

V

S

S

K

T

V

I

S

T

G

A

R

Q

L

L

K

P

K

E

E

Q

Y

L

D

H

C

A

F

S

E

D

A

L

L

L

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T

S

V

I

S

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

D

D

E

E

L

L

E

E

N

P

T

Q

K

R

R

R

G

N

C

A

Y

W

A

C

G

G

A

S

V

I

G

G

Y

Y

F

L

S

S

Y

F

D

C

G

G

N

N

M

M

D

D

T

T

C

S

I

I

A

T

I

I

R

R

T

T

L

L

V

T

I

A

K

I

E

N

G

G

K

Q

A

I

Y

F

A

C

Q

S

A

A

G

G

V

I

V

V

Y

A

D

D

S

S

I

Q

P

E

E

E

N

A

E

E

Y

Y

E

Q

E

E

S

T

M

F

N

D

K

K

-

V

S

N

Q

R

I

I

L

L

K

K

E

Q

V

L

S36 (= S49) binding L-tryptophan
E258 (= E282) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
K274 (≠ A298) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
G275 (= G299) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
R311 (= R335) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
R316 (≠ K340) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
S322 (≠ T346) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
H339 (= H363) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.

1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
30% identity, 91% coverage: 45:474/475 of query aligns to 30:435/437 of 1k0eA

query
sites
1k0eA

F

M

L

L

L
|
L

E
x
H

S
|
S

N

G

L

Y

F

A

S

D

E

H

S

P

K
x
Y

G

S

R

R

Y
x
F

S

D

F

I

I

V

G

V

A

A

N

E

P

P

Y

I

A

C

I

T

I

L

K

T

G

T

F

F

S

G

N

K

N

E

T

T

E

V

I

V

I

S

R

E

N

S

G

E

Y

K

V

R

K

T

V

T

E

T

D

D

K

D

F

P

L

L

K

Q

V

V

A

L

R

Q

K

Q

L

V

T

L

E

D

E

R

G

A

K

D

I

I

E

R

K

P

S

T

K

H

-

N

-

E

-

D

Y

L

P

P

F

F

C

Q

G

G

G

A

V

L

G

G

Y

L

V

F

G

G

Y

Y

D

D

I

L

I

G

K

R

Q

R

Y

F

E

E

K

S

I

L

P

P

S

E

L

I

N

A

E

E

D

Q

D

D

L

I

K

V

I

L

P

P

D

D

A

M

I

A

L

V

M

G

F

I

Y

Y

K

D

K

W

I

A

I

L

C

I

Y

V

D

D

H

H

V

Q

K

R

N

H

S

T

I

V

V

S

Y

L

I

L

-

S

-

H

-

N

-

D

Y

V

N

N

V

A

F

R

R

R

E

A

D

W

D

L

K

E

S

S

Y

Q

K

Q

E

F

I

S

K

P

Q

Q

E

E

L

D

F

F

E

T

L

L

S

T

E

-

I

-

S

-

K

-

T

-

R

-

E

-

M

-

H

-

K

-

L

-

P

-

E

-

S

-

H

-

N

-

P

-

A

S

N

D

I

W

S

Q

S

S

N

N

F

M

T

T

K

R

D

E

E

Q

F

Y

C

G

K

E

M

K

V

F

K

R

K

Q

A

V

Q

Q

E

E

Y

Y

I

L

V

H

K

S

G

G

D

D

I

C

F

Y

Q

Q

M

V

V

N

P

L

S

A

Q

Q

R

R

F

F

V

H

M

A

D

T

I

Y

D

S

E

G

R

D

P

E

F

W

N

Q

V

A

Y

F

R

L

R

Q

L

L

R

N

G

Q

K

A

N

N

P

R

S

A

P
|
P

Y
x
F

L
x
S

F

A

Y

F

I

L

D

R

F

L

N

E

D

Q

F

G

Q

A

V

I

T

L

G

S

S

L

S

S

P

P

E
|
E

S

R

L

F

V

I

C

L

V

C

F

D

K

N

D

S

R

E

V

I

I

Q

T

T

N

R

P

P

I

I

A
x
K

G

G

T

T

R

L

P

P

R

R

G

A

K

-

N

-

E

-

Q

-

E

-

D

-

I

-

K

-

L

-

K

-

S

-

E

-

L

-

I

-

N

N

D

S

E

A

K

K

E

D

I

R

A

A

E
|
E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

M

R

R

N

N

D

D

I

I

G

G

K

R

I

V

S

A

E

V

F

A

G

G

T

S

V

V

T

K

V

V

D

P

K

E

F

L

M

F

E

V

V

V

E

E

L

P

Y

F

S

P

H

A

V

V

M

H

H
|
H

I

L

V

V

S

S

K

T

V

I

S

T

G

A

R

Q

L

L

K

P

K

E

E

Q

Y

L

D

H

C

A

F

S

E

D

A

L

L

L

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T
x
S

V

I

S

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

D

D

E

E

L

L

E

E

N

P

T

Q

K

R

R

R

G

N

C

A

Y
x
W

A

C

G

G

A

S

V

I

G

G

Y

Y

F

L

S

S

Y

F

D

C

G

G

N

N

M

M

D

D

T

T

C

S

I

I

A

T

I

I

R
|
R

T

T

L

L

V

T

I

A

K

I

E

N

G

G

K

Q

A

I

Y

F

A

C

Q

S

A

A

G

G

G
|
G

V

I

V

V

Y

A

D

D

S

S

I

Q

P

E

E

E

N

A

E

E

Y

Y

E

Q

E
|
E

S

T

M

F

N

D

K
|
K

-

V

S

N

Q

R

I

I

L

L

K

K

E

Q

V

L

active site: E256 (= E282), K272 (≠ A298), E286 (= E326), H323 (= H363), S350 (≠ T390), W374 (≠ Y414), R394 (= R434), G410 (= G450), E423 (= E463), K427 (= K467)
binding tryptophan: L32 (= L47), H33 (≠ E48), S34 (= S49), Y41 (≠ K56), F44 (≠ Y59), P238 (= P264), F239 (≠ Y265), S240 (≠ L266)

1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
28% identity, 91% coverage: 45:474/475 of query aligns to 32:415/415 of 1k0gB

query
sites
1k0gB

F

M

L

L

L
|
L

E
x
H

S
|
S

N

G

L

Y

F

A

S

D

E

H

S

P

K
x
Y

G
x
S

R
|
R

Y
x
F

S

D

F

I

I

V

G

V

A

A

N

E

P

P

Y

I

A

C

I

T

I

L

K

T

G

T

F

F

S

G

N

K

N

E

T

T

E

V

I

V

I

S

R

E

N

S

G

E

Y

K

V

R

K

T

V

T

E

T

D

D

K

D

F

P

L

L

K

Q

V

V

A

L

R

Q

K

Q

L

V

T

L

E

D

E

R

G

A

K

D

I

I

E

R

K

P

S

T

K

H

-

N

-

E

-

D

Y

L

P

P

F

F

C

Q

G

G

G

A

V

L

G

G

Y

L

V

F

G

G

Y
|
Y

D
|
D

I

L

I

G

K
x
R

Q

R

Y

F

E

E

K

S

I

L

P

P

S

E

L

I

N

A

E

E

D

Q

D

D

L

I

K

V

I

L

P

P

D

D

A

M

I

A

L

V

M

G

F

I

Y

Y

K

D

K

W

I

A

I

L

C

I

Y

V

D

D

H

H

V

Q

K

R

N

H

S

T

I

V

V

S

Y

L

I

L

-

S

-

H

-

N

-

D

Y

V

N

N

V

A

F

R

R

R

E

A

D

W

D

L

K

E

S

S

Y

Q

K

Q

E

F

I

S

K

P

Q

Q

E

E

L

D

F

F

E

T

L

L

S

T

E

-

I

-

S

-

K

-

T

-

R

-

E

-

M

-

H

-

K

-

L

-

P

-

E

-

S

-

H

-

N

-

P

-

A

S

N

D

I

W

S

Q

S

S

N

N

F

M

T

T

K

R

D

E

E

Q

F

Y

C

G

K

E

M

K

V

F

K

R

K

Q

A

V

Q

Q

E

E

Y

Y

I

L

V

H

K

S

G

G

D

D

I

C

F

Y

Q

Q

M

V

V

N

P

L

S

A

Q

Q

R

R

F

F

V

H

M

A

D

T

I

Y

D

S

E

G

R

D

P

E

F

W

N

Q

V

A

Y

F

R

L

R

Q

L

L

R

N

G

Q

K

A

N

N

P

R

S

A

P
|
P

Y
x
F

L

S

F

A

Y

F

I

L

D

R

F

L

N

E

D

Q

F

G

Q

A

V

I

T

L

G

S

S

L

S

S

P

P

E
|
E

S

R

L

F

V

I

C

L

V

C

F

D

K

N

D

S

R

E

V

I

I

Q

T

T

N

R

P

P

I

I

A
x
K

G

G

T

-

R

-

P

-

R

-

G

-

K

-

N

-

E

-

Q

-

E

-

D

-

I

-

K

-

L

-

K

-

S

-

E

-

L

-

I

-

N

-

D

-

E

-

K

-

E

-

I

-

A

A

E
|
E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

M

R

R

N

N

D

D

I

I

G

G

K

R

I

V

S

A

E

V

F

A

G

G

T

S

V

V

T

K

V

V

D

P

K

E

F

L

M

L

E

-

V

-

E

-

L

-

Y

-

S

-

H

-

V

-

M

-

H

-

I

-

V

V

S

S

K

T

V

I

S

T

G

A

R

Q

L

L

K

P

K

E

E

Q

Y

L

D

H

C

A

F

S

E

D

A

L

L

L

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T
x
S

V

I

S

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

D
|
D

E

E

L

L

E

E

N

P

T

Q

K
x
R

R

R

G

N

C

A

Y
x
W

A

C

G

G

A

S

V

I

G

G

Y

Y

F

L

S

S

Y

F

D

C

G

G

N

N

M

M

D

D

T

T

C

S

I

I

A

T

I

I

R
|
R

T

T

L

L

V

T

I

A

K

I

E

N

G

G

K

Q

A

I

Y

F

A

C

Q

S

A

A

G

G

G
|
G

V

I

V

V

Y

A

D

D

S

S

I

Q

P

E

E

E

N

A

E

E

Y

Y

E

Q

E
|
E

S

T

M

F

N

D

K
|
K

-

V

S

N

Q

R

I

I

L

L

K

K

E

Q

V

L

active site: E258 (= E282), K274 (≠ A298), E277 (= E326), S330 (≠ T390), W354 (≠ Y414), R374 (= R434), G390 (= G450), E403 (= E463), K407 (= K467)
binding phosphate ion: Y112 (= Y122), D113 (= D123), R116 (≠ K126), D344 (= D404), R350 (≠ K410)
binding tryptophan: L34 (= L47), H35 (≠ E48), S36 (= S49), Y43 (≠ K56), S44 (≠ G57), R45 (= R58), F46 (≠ Y59), P240 (= P264), F241 (≠ Y265)

1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
28% identity, 91% coverage: 45:474/475 of query aligns to 32:418/420 of 1k0gA

query
sites
1k0gA

F

M

L

L

L
|
L

E
x
H

S
|
S

N

G

L

Y

F

A

S

D

E

H

S

P

K
x
Y

G
x
S

R

R

Y
x
F

S

D

F

I

I

V

G

V

A

A

N

E

P

P

Y

I

A

C

I

T

I

L

K

T

G

T

F

F

S

G

N

K

N

E

T

T

E

V

I

V

I

S

R

E

N

S

G

E

Y

K

V

R

K

T

V

T

E

T

D

D

K

D

F

P

L

L

K

Q

V

V

A

L

R

Q

K

Q

L

V

T

L

E

D

E

R

G

A

K

D

I

I

E

R

K

P

S

T

K

H

-

N

-

E

-

D

Y

L

P

P

F

F

C

Q

G

G

G

A

V

L

G

G

Y

L

V

F

G

G

Y

Y

D
|
D

I

L

I

G

K
x
R

Q

R

Y

F

E

E

K

S

I

L

P

P

S

E

L

I

N

A

E

E

D

Q

D

D

L

I

K

V

I

L

P

P

D

D

A

M

I

A

L

V

M

G

F

I

Y

Y

K

D

K

W

I

A

I

L

C

I

Y

V

D

D

H

H

V

Q

K

R

N

H

S

T

I

V

V

S

Y

L

I

L

-

S

-

H

-

N

-

D

Y

V

N

N

V

A

F

R

R

R

E

A

D

W

D

L

K

E

S

S

Y

Q

K

Q

E

F

I

S

K

P

Q

Q

E

E

L

D

F

F

E

T

L

L

S

T

E

-

I

-

S

-

K

-

T

-

R

-

E

-

M

-

H

-

K

-

L

-

P

-

E

-

S

-

H

-

N

-

P

-

A

S

N

D

I

W

S

Q

S

S

N

N

F

M

T

T

K

R

D

E

E

Q

F

Y

C

G

K

E

M

K

V

F

K

R

K

Q

A

V

Q

Q

E

E

Y

Y

I

L

V

H

K

S

G

G

D

D

I

C

F

Y

Q

Q

M

V

V

N

P

L

S

A

Q

Q

R

R

F

F

V

H

M

A

D

T

I

Y

D

S

E

G

R

D

P

E

F

W

N

Q

V

A

Y

F

R

L

R

Q

L

L

R

N

G

Q

K

A

N

N

P

R

S

A

P
|
P

Y
x
F

L
x
S

F

A

Y

F

I

L

D

R

F

L

N

E

D

Q

F

G

Q

A

V

I

T

L

G

S

S

L

S

S

P

P

E
|
E

S

R

L

F

V

I

C

L

V

C

F

D

K

N

D

S

R

E

V

I

I

Q

T

T

N

R

P

P

I

I

A

-

G

-

T

-

R

-

P

-

R

-

G

-

K

-

N

-

E

-

Q

-

E

-

D

-

I

-

K

-

L

-

K

-

S

-

E

-

L

-

I

-

N

-

D

-

E

-

K
|
K

E

D

I

R

A

A

E
|
E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

M

R

R

N

N

D

D

I

I

G

G

K

R

I

V

S

A

E

V

F

A

G

G

T

S

V

V

T

K

V

V

D

P

K

-

F

-

M

-

E

-

V

-

E

E

L

L

Y

F

S

V

H

-

V

-

M

-

H

-

I

L

V

V

S

S

K

T

V

I

S

T

G

A

R

Q

L

L

K

P

K

E

E

Q

Y

L

D

H

C

A

F

S

E

D

A

L

L

L

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T
x
S

V

I

S

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

D
|
D

E

E

L

L

E

E

N

P

T

Q

K
x
R

R

R

G

N

C

A

Y
x
W

A

C

G

G

A

S

V

I

G

G

Y

Y

F

L

S

S

Y

F

D

C

G

G

N

N

M

M

D

D

T

T

C

S

I

I

A

T

I

I

R
|
R

T

T

L

L

V

T

I

A

K

I

E

N

G

G

K

Q

A

I

Y

F

A

C

Q

S

A

A

G

G

G
|
G

V

I

V

V

Y

A

D

D

S

S

I

Q

P

E

E

E

N

A

E

E

Y

Y

E

Q

E
|
E

S

T

M

F

N

D

K
|
K

-

V

S

N

Q

R

I

I

L

L

K

K

E

Q

V

L

active site: E258 (= E282), K274 (= K322), E278 (= E326), S333 (≠ T390), W357 (≠ Y414), R377 (= R434), G393 (= G450), E406 (= E463), K410 (= K467)
binding phosphate ion: D113 (= D123), R116 (≠ K126), D347 (= D404), R353 (≠ K410)
binding tryptophan: L34 (= L47), H35 (≠ E48), S36 (= S49), Y43 (≠ K56), S44 (≠ G57), F46 (≠ Y59), P240 (= P264), F241 (≠ Y265), S242 (≠ L266)

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
28% identity, 90% coverage: 43:471/475 of query aligns to 227:666/673 of 8hx8A

query
sites
8hx8A

N

T

K

T

F

F

L

W

L
|
L

E
x
D

S
|
S

N

S

L

S

F

V

S

L

E

E

S

G

K

A

G
x
S

R

R

Y
x
F

S

S

F

F

I

L

G

G

-

D

-

R

-

G

-

P

-

L

-

A

-

E

-

Y

-

L

-

T

-

Y

-

R

-

V

A

A

N

D

P

G

Y

V

A

V

I

S

I

V

K

R

G

G

F

S

S

D

N

G

N

T

T

T

E

T

I

R

I

T

R

R

N

R

G

P

Y

F

V

F

K

N

K

Y

V

L

E

E

D

E

K

Q

F

L

L

E

K

R

V

R

A

R

R

V

K

P

L

V

T

A

E

P

E

E

G

L

K

P

I

F

E

E

K

-

S

-

K

-

Y

-

P

-

F

F

C

N

G

L

G

G

G

Y

V

V

G

G

Y

Y

V

L

G

G

Y

Y

D

E

I

L

I

K

K

A

Q

E

Y

T

E

T

K

G

I

D

P

P

S

A

L

H

N

R

E

S

D

P

D

H

L

-

K

-

I

-

P

P

D

D

A

A

I

A

L

F

M

L

F

F

Y

A

K

D

K

R

I

A

I

I

C

A

Y

L

D

D

H

H

V

-

K

Q

N

E

S

G

I

C

V

C

Y

Y

I

L

Y

L

N

A

V

L

F

D

R

R

-

R

-

G

E

H

D

D

K

G

S

A

Y

R

K

A

E

W

I

L

K

R

Q

E

E

T

L

A

F

E

E

T

L

L

S

T

E

G

I

L

S

A

K

V

K

R

T

A

R

-

E

-

M

-

H

-

K

-

L

-

P

P

E

A

S

E

H

P

N

T

P

P

A

A

N

M

I

V

-

F

-

G

-

A

-

G

-

F

-

G

-

P

-

L

-

A

S

R

S

A

N

R

F

H

T

D

K

K

D

D

E

A

F

Y

C

L

K

K

M

R

V

I

K

D

K

E

A

C

Q

L

E

K

Y

E

I

I

V

R

K

N

G

G

D

E

I

S

F

Y

Q

E

M

I

V

C

P

L

S

T

Q

N

R

M

F

V

V

T

M

A

D

P

I

T

D

E

E

A

R

T

P

A

F

L

N

P

V

L

Y

Y

R

S

R

A

L

L

R

R

G

A

K

I

N

S

P

P

S

V

P
|
P

Y
|
Y

L
x
G

F

A

Y

L

I

L

D

E

F

F

N

P

D

E

F

L

Q

S

V

V

T

L

G

S

S

A

S

S

P

P

E

E

S

R

L

F

V

L

C

T

V

I

F

G

K

A

D

D

R

G

-

G

V

V

I

E

T

S

N

K

P

P

I

I

A

K

G

G

T

T

R

R

P

P

R

R

G

G

K

G

N

T

E

A

Q

E

E

E

D

D

I

E

K

R

L

L

K

R

S

A

E

D

L

L

I

A

N

G

D

R

E

E

K

K

E

D

I

R

A

A

E
|
E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

V

R

R

N

N

D

D

I

L

G

N

K

S

I

V

S

C

E

A

F

I

G

G

T

S

V

V

T

H

V

V

D

P

K

R

F

L

M

F

E

E

V

V

E

E

L

T

Y

Y

S

A

H

P

V

V

M

H

H

Q

I

L

V

V

S

S

K

T

V

I

S

R

G

G

R

R

L

L

K

R

K

P

E

G

Y

T

D

S

C

T

F

A

E

A

A

C

L

V

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T

S

V

M

S

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

I

I

D

D

E

R

L

L

E

E

N

E

T

G

K

P

R

R

G

G

C

V

Y

Y

A

S

G

G

A

A

V

L

G
|
G

Y

W

F

F

S

A

Y

L

D

S

G

G

N

A

M

A

D

D

T

L

C

S

I

I

A

V

I

I

R

R

T

T

L

I

V

V

I

L

K

A

E

D

G

G

K

Q

A

A

Y

E

A

F

Q

G

A

V

G

G

G

A

V

I

V

V

Y

S

D

L

S

S

I

D

P

Q

E

E

N

E

E
|
E

Y

F

E

T

E
|
E

S

T

M

V

N

V

K

K

S

A

Q

R

I

A

L

M

binding magnesium ion: E521 (= E326), E655 (= E460), E658 (= E463)
binding tryptophan: L231 (= L47), D232 (≠ E48), S233 (= S49), S241 (≠ G57), F243 (≠ Y59), P458 (= P264), Y459 (= Y265), G460 (≠ L266), G614 (= G419)

Sites not aligning to the query:

binding magnesium ion: 175, 177

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
29% identity, 90% coverage: 43:471/475 of query aligns to 185:627/632 of 8hx9A

query
sites
8hx9A

N

T

K

T

F

F

L

W

L
|
L

E
x
D

S
|
S

N

S

L

S

F

V

S

L

E

E

S

G

K

A

G
x
S

R

R

Y
x
F

S

S

F

F

I

L

G

G

-

D

-

R

-

G

-

P

-

L

-

A

-

E

-

Y

-

L

-

T

-

Y

-

R

-

V

A

A

N

D

P

G

Y

V

A

V

I

S

I

V

K

R

G

G

F

S

S

D

N

G

N

T

T

T

E

T

I

R

I

T

R

R

N

R

G

P

Y

F

V

F

K

N

K

Y

V

L

E

E

D

E

K

Q

F

L

L

E

K

R

V

R

A

R

R

V

K

P

L

V

T

A

E

P

E

E

G

L

K

P

I

F

E

E

K

-

S

-

K

-

Y

-

P

-

F

F

C

N

G

L

G

G

G

Y

V

V

G

G

Y

Y

V

L

G

G

Y

Y

D

E

I

L

I

K

K

A

Q

E

Y

T

E

T

K

G

I

D

P

P

S

A

L

H

N

R

E

S

D

P

D

H

L

-

K

-

I

-

P

P

D

D

A

A

I

A

L

F

M

L

F

F

Y

A

K

D

K

R

I

A

I

I

C

A

Y

L

D

D

H

H

V

-

K

Q

N

E

S

G

I

C

V

C

Y

Y

I

L

Y

L

N

A

V

L

F

D

R

R

-

R

-

G

E

H

D

D

K

G

S

A

-

R

-

A

-

W

Y

L

K

R

E

E

I

T

K

A

Q

E

E

T

L

L

F

T

E

G

L

L

S

A

E

V

I

R

S

A

K

P

T

T

R

P

E

A

M

M

-

V

H

F

K

G

L

I

P

P

E

E

S

A

H

A

N

A

P

G

A

F

N

G

-

P

-

L

I

A

S

R

S

A

N

R

F

H

T

D

K

K

D

D

E

A

F

Y

C

L

K

K

M

R

V

I

K

D

K

E

A

C

Q

L

E

K

Y

E

I

I

V

R

K

N

G

G

D

E

I

S

F

Y

Q

E

M

I

V

C

P

L

S

T

Q

N

R

M

F

V

V

T

M

A

D

P

I

T

D

E

E

A

R

T

P

A

F

L

N

P

V

L

Y

Y

R

S

R

A

L

L

R

R

G

A

K

I

N

S

P

P

S

V

P
|
P

Y
|
Y

L
x
G

F

A

Y

L

I

L

D

E

F

F

N

P

D

E

F

L

Q

S

V

V

T

L

G

S

S

A

S

S

P

P

E

E

S

R

L

F

V

L

C

T

V

I

F

G

K

A

D

D

R

G

-

G

V

V

I

E

T

S

N

K

P

P

I
|
I

A
x
K

G
|
G

T
|
T

R

R

P

P

R

R

G

G

K

G

N

T

E

A

Q

E

E

E

D

D

I

E

K

R

L

L

K

R

S

A

E

D

L

L

I

A

N

G

D

R

E

E

K

K

E

D

I

R

A

A

E

E

H

N

S

L

M

M

L

I

L

V

D

D

L

L

A

V

R

R

N

N

D

D

I

L

G

N

K

S

I

V

S

C

E

A

F

I

G

G

T

S

V

V

T

H

V

V

D

P

K

R

F

L

M

F

E

E

V

V

E

E

L

T

Y

Y

S

A

H

P

V

V

M

H

H

Q

I

L

V

V

S

S

K

T

V

I

S

R

G

G

R

R

L

L

K

R

K

P

E

G

Y

T

D

S

C

T

F

A

E

A

A

C

L

V

K

R

S

A

C

A

L

F

P

P

A

G

G

G

T

S

V
x
M

S

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

I

I

D

D

E

R

L

L

E

E

N

E

T

G

K

P

R

R

G

G

C

V

Y
|
Y

A

S

G

G

A

A

V
x
L

G
|
G

Y

W

F

F

S

A

Y

L

D

S

G

G

N

A

M

A

D

D

T

L

C

S

I

I

A

V

I

I

R
|
R

T

T

L

I

V

V

I

L

K

A

E

D

G

G

K

Q

A

A

Y

E

A

F

Q

G

A
x
V

G
|
G

G
x
A

V

I

V

V

Y

S

D

L

S

S

I

D

P

Q

E

E

N

E

E

E

Y

F

E

T

E
|
E

S

T

M

V

N

V

K
|
K

S

A

Q

R

I

A

L

M

binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (= I297), K454 (≠ A298), G455 (= G299), T456 (= T300), M547 (≠ V391), Y570 (= Y414), R590 (= R434), V603 (≠ A447), G604 (= G448), G605 (= G449), A606 (≠ G450), E619 (= E463), K623 (= K467)
binding tryptophan: L189 (= L47), D190 (≠ E48), S191 (= S49), S199 (≠ G57), F201 (≠ Y59), P419 (= P264), Y420 (= Y265), G421 (≠ L266), L574 (≠ V418), G575 (= G419)

Sites not aligning to the query:

binding magnesium ion: 134, 135

2fn1A Crystal structures of yersinia enterocolitica salicylate synthase (irp9) in complex with the reaction products salicylate and pyruvate (see paper)
28% identity, 55% coverage: 205:467/475 of query aligns to 137:398/408 of 2fn1A

query
sites
2fn1A

P

P

A

L

N

A

I

V

S

D

S

T

N

A

F

L

T

N

K

G

D

E

E

A

F

Y

C

K

K

Q

M

Q

V

V

K

A

K

R

A

A

Q

V

E

A

Y

E

I

I

V

R

K

R

G

G

D

E

I

Y

F

V

Q
x
K

M

V

V

I

P

V

S

S

Q

R

R

A

F

I

V

P

M

L

D

P

-

S

-

R

I

I

D

D

E

M

R

P

P

A

F

T

N

L

V

L

Y

Y

R

G

R

R

L

Q

R

A

G

N

K

T

N

P

P

V

S

R

P

S

Y

F

L

M

F

F

Y

R

I

Q

D

E

F

G

N

R

D

E

F

-

Q

-

V

A

T

L

G

G

S

F

S

S

P

P

E
|
E

S

L

L

V

V

M

C

S

V

V

F

T

K

G

D

N

R

K

V

V

I

V

T

T

N

E

P

P

I

L

A
|
A

G

G

T

T

R

R

P

D

R

R

G

M

K

G

N

N

E

P

Q

E

E

H

D

N

I

K

K

A

L

K

K

E

S

A

E

E

L

L

I

L

N

H

D

D

E

S

K

K

E

E

I

V

A

L

E
|
E

H

H

S

I

M

L

L

S

L

V

D

K

L

E

A

A

R

I

N

A

D

E

I

L

G

E

K

A

I

V

S

C

E

L

F

P

G

G

T

S

V

V

T

V

V

V

D

E

K

D

F

L

M

M

E

S

V

V

E

R

L

Q

Y

R

S

G

H

S

V

V

M

Q

H
|
H

I

L

V

G

S

S

K

G

V

V

S

S

G

G

R

Q

L

L

K

A

K

E

E

N

Y

K

D

D

C

A

F

W

E

D

A

A

L

F

K

T

S

V

C

L

L

F

P

P

A

S

G

I

T
|
T

V

A

S

S

G

G

A

I

P

P

K

K

I

N

R

A

A

A

M

L

E

N

I

A

I

I

D

M

E

Q

L

I

E

E

N

K

T

T

K

P

R

R

G

E

C

L

Y
|
Y

A

S

G

G

A

A

V

I

G

L

Y

L

F

L

S

D

Y

-

D

D

G

T

N

R

M

F

D

D

T

A

C

A

I

L

A

V

I
x
L

R
|
R

T

S

L

V

V

F

I

Q

K

D

E

S

G

Q

K

R

A

C

Y

W

A

I

Q

Q

A
|
A

G
|
G

G

A

G
|
G

V

I

Y

A

D

Q

S

S

I

T

P

P

E

E

N

R

E

E

Y

L

E

T

E
|
E

S

T

M

R

N

E

K
|
K

active site: K167 (≠ Q235), E214 (= E282), A230 (= A298), E258 (= E326), H295 (= H363), T322 (= T390), Y346 (= Y414), R365 (= R434), G381 (= G450), E394 (= E463), K398 (= K467)
binding magnesium ion: E258 (= E326), E394 (= E463)
binding pyruvic acid: Y346 (= Y414), L364 (≠ I433), R365 (= R434), A378 (= A447), G379 (= G448), K398 (= K467)

Query Sequence

>WP_010966440.1 NCBI__GCF_000008765.1:WP_010966440.1
MYNISEEQFNDNKKLKKAFQVIYEENGDEITPISLFYNLEGKNKFLLESNLFSESKGRYS
FIGANPYAIIKGFSNNTEIIRNGYVKKVEDKFLKVARKLTEEGKIEKSKYPFCGGGVGYV
GYDIIKQYEKIPSLNEDDLKIPDAILMFYKKIICYDHVKNSIVYIYNVFREDDKSYKEIK
QELFELSEISKKTREMHKLPESHNPANISSNFTKDEFCKMVKKAQEYIVKGDIFQMVPSQ
RFVMDIDERPFNVYRRLRGKNPSPYLFYIDFNDFQVTGSSPESLVCVFKDRVITNPIAGT
RPRGKNEQEDIKLKSELINDEKEIAEHSMLLDLARNDIGKISEFGTVTVDKFMEVELYSH
VMHIVSKVSGRLKKEYDCFEALKSCLPAGTVSGAPKIRAMEIIDELENTKRGCYAGAVGY
FSYDGNMDTCIAIRTLVIKEGKAYAQAGGGVVYDSIPENEYEESMNKSQILKEVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory