SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010966867.1 NCBI__GCF_000008765.1:WP_010966867.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 96% coverage: 22:568/572 of query aligns to 41:571/575 of P9WKJ5

query
sites
P9WKJ5

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C64 (≠ G45) binding [2Fe-2S] cluster
D96 (= D77) binding Mg(2+)
C137 (= C118) binding [2Fe-2S] cluster
D138 (= D119) binding Mg(2+)
K139 (= K120) binding via carbamate group; modified: N6-carboxylysine
C214 (= C187) binding [2Fe-2S] cluster
E465 (= E451) binding Mg(2+)
S491 (= S478) mutation to A: Loss of catalytic activity.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
35% identity, 96% coverage: 22:568/572 of query aligns to 28:558/562 of 6ovtA

query
sites
6ovtA

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binding fe2/s2 (inorganic) cluster: C51 (≠ G45), G84 (= G78), C124 (= C118), D125 (= D119), A200 (= A186), C201 (= C187)
binding magnesium ion: D83 (= D77), D125 (= D119), K126 (= K120), E452 (= E451)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 96% coverage: 20:568/572 of query aligns to 75:603/608 of Q9LIR4

query
sites
Q9LIR4

G

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G

Q

E

Y

F

E

V

R

S

G

G

E

S

I

I

T

S

K

D

E

E

Q

Q

Y

R

E

K

H

T

Y

V

K

L

Q

H

N

H

A

S

C

C

P

P

S

G

C

A

G

G

A

A

C
|
C

S

G

F

G

M

M

G

Y

T

T

A

A

S

N

T

T

M

M

Q

A

V

S

M

A

S

I

E

E

A

A

L

M

G

G

I

M

A

S

L

L

P

P

G

Y

T

S

A

S

L

S

I

I

P

P

V

A

T

-

S

E

K

D

E

P

L

L

K

K

L

L

-

D

A

E

A

C

K

R

N

L

A

A

G

G

K

K

Q

Y

I

L

L

L

K

E

L

L

I

L

E

K

L

M

N

D

I

L

K

K

P

P

S

R

E

D

I

I

M

I

T

T

K

P

K

K

A

S

F

L

E

R

N

N

A

A

I

M

M

V

V

S

H

V

A

M

A

A

I

L

A

G

G

G

S

S

S

T

N

N

C

A

L

V

L

L

H

H

I

L

P

I

A

A

I

I

A

A

H

R

E

S

L

V

G

G

M

L

D

E

I

L

E

T

P

L

E

D

L

D

F

F

D

Q

E

K

I

V

H

S

K

D

K

A

I

V

P

P

Y

F

I

L

L

A

N

D

I

L

R

K

P

P

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S

G

G

F

K

Y

Y

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V

G

M

S

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Y

D

F

I

W

H

N

K

A

I

G

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T

P

P

A

A

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M

L

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R

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Y

I

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L

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-

L

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G

F

L

L

M

H

D

L

G

D

D

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C

M

M

T

T

V

V

T

T

G

G

K

Q

T

T

L

L

G

A

E

Q

N

N

L

L

E

E

D

N

L

-

K

-

N

-

S

-

G

-

Y

-

Y

-

E

-

E

-

H

-

D

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K

-

L

-

I

V

T

P

S

S

L

L

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-

I

T

K

E

K

G

E

Q

D

E

V

I

I

I

R

R

T

P

K

L

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N

N

P

P

I

I

Q

K

S

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Q

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G

G

A

H

I

I

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I

I

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L

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D

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L

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G

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V

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K

H

I

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S

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K

K

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L

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Y

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F

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P

A

A

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L

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V

F

F

N

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C

G

E

E

E

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D

S

A

M

I

L

K

A

S

A

V

I

L

S

T

A

K

D

N

P

I

M

K

S

-

F

P

K

G

G

D

T

A

V

V

V

F

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

S

G

-

P

G

G

M

M

P

P

E
|
E

M

M

F

L

Y

T

T

P

T

T

E

S

A

A

I

I

A

M

S

G

D

-

H

A

E

G

L

L

V

G

D

K

S

E

T

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

A

G

T

S

R

H

G

G

P

F

A

V

I

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

S

Q

E

E

G

G

G

G

P

P

I

I

A

G

F

L

V

I

Q

K

E

N

G

G

D

D

L

I

I

I

K

T

I

I

D

D

I

I

P

G

A

K

R

K

K

R

L

I

D

D

I

-

I

-

G

-

T

-

N

-

G

-

I

T

E

Q

K

V

S

S

E

P

K

E

E

E

I

-

E

-

N

-

I

-

L

M

K

N

E

D

R

R

S

R

Q

K

N

K

W

W

V

T

K

A

P

P

A

A

P

Y

R

K

Y

V

T

N

K

R

G

G

I

V

L

L

G

Y

L

K

Y

Y

T

I

R

K

C

N

A

V

S

Q

S

S

P

A

M

S

K

D

G

G

C100 (≠ G45) binding [2Fe-2S] cluster
C173 (= C118) binding [2Fe-2S] cluster
D174 (= D119) binding Mg(2+)
C245 (= C187) binding [2Fe-2S] cluster
E497 (= E451) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
34% identity, 96% coverage: 20:568/572 of query aligns to 37:565/570 of 8hs0A

query
sites
8hs0A

G

G

S

V

G

G

W

L

K

S

S

D

E

D

E

D

L

L

N

L

K

K

A

P

Q

Q

I

I

I

G

I

I

E

S

S

S

S

V

F

W

G

Y

H

E

S

G

H

N

P

T

G
x
C

S

N

A

M

H

H

L

L

D

L

T

K

L

L

V

S

D

E

E

A

A

V

F

K

K

E

S

G

I

V

D

E

D

N

N

A

G

G

G

M

R

V

G

G

A

F

K

R

Y

F

F

N

V

T

T

I

D

G

I

V

C

S

D
|
D

G
x
A

E

I

T

S

Q

M

G

G

H

T

D

R

G

G

M

M

N

C

Y

F

S

S

L

L

A

Q

S

S

R

R

D

D

I

L

M

I

T

A

N

D

L

S

M

I

E

E

I

T

H

V

V

M

Q

S

A

A

T

Q

P

W

F

Y

D

D

A

G

G

N

I

I

F

S

I

I

A

P

S

G

C
|
C

D
|
D

K
|
K

A

N

V

M

P

P

A

G

H

T

L

I

M

M

A

A

I

M

A

G

R

R

L

L

D

N

M

R

P

P

S

G

I

I

L

M

V

V

P

Y

G

G

G

G

I

T

M

I

N

K

A

P

G

G

P

H

N

F

M

-

L

-

T

-

L

-

E

-

Q

Q

I

D

G

K

T

T

Y

Y

N

D

-

I

-

W

-

S

-

A

-

F

-

Q

-

S

-

Y

A

G

Q

E

Y

F

E

V

R

S

G

G

E

S

I

I

T

S

K

D

E

E

Q

Q

Y

R

E

K

H

T

Y

V

K

L

Q

H

N

H

A

S

C

C

P

P

S

G

C

A

G

G

A

A

C
|
C

S

G

F

G

M

M

G

Y

T

T

A

A

S

N

T

T

M

M

Q

A

V

S

M

A

S

I

E

E

A

A

L

M

G

G

I

M

A

S

L

L

P

P

G

Y

T

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A

S

L

S

I

I

P

P

V

A

T

-

S

E

K

D

E

P

L

L

K

K

L

L

-

D

A

E

A

C

K

R

N

L

A

A

G

G

K

K

Q

Y

I

L

L

L

K

E

L

L

I

L

E

K

L

M

N

D

I

L

K

K

P

P

S

R

E

D

I

I

M

I

T

T

K

P

K

K

A

S

F

L

E

R

N

N

A

A

I

M

M

V

V

S

H

V

A

M

A

A

I

L

A

G

G

G

S

S

S

T

N
|
N

C

A

L

V

L

L

H
|
H

I

L

P

I

A

A

I

I

A

A

H

R

E

S

L

V

G

G

M

L

D

E

I

L

E

T

P

L

E

D

L

D

F

F

D

Q

E

K

I

V

H

S

K

D

K

A

I

V

P

P

Y

F

I

L

L

A

N

D

I

L

R

K

P

P

S

S

G

G

F

K

Y

Y

P

V

G

M

S

E

Y

D

F

I

W

H

N

K

A

I

G

G

G

G

V

T

P

P

A

A

I

V

M

L

E

R

E

Y

I

L

K

L

E

E

-

L

-

G

F

L

L

M

H

D

L

G

D

D

V

C

M

M

T

T

V

V

T

T

G

G

K

Q

T

T

L

L

G

A

E

Q

N

N

L

L

E

E

D

N

L

-

K

-

N

-

S

-

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

V

T

P

S

S

L

L

G

-

I

T

K

E

K

G

E

Q

D

E

V

I

I

I

R

R

T

P

K

L

E

S

N

N

P

P

I

I

Q

K

S

E

Q

T

G

G

A

H

I

I

A

Q

I

I

L

L

K

R

G

G

N

D

L

L

A

A

P

P

G

D

G

G

A

S

V

V

V

A

K

K

H

I

S

T

A

G

I

-

S

-

K

K

K

E

L

G

M

L

Q

Y

V

F

V

S

L

G

K

P

A

A

R

L

V

V

F

F

N

E

C

G

E

E

E

E

D

S

A

M

I

L

K

A

S

A

V

I

L

S

T

A

K

D

N

P

I

M

K

S

-

F

P

K

G

G

D

T

A

V

V

V

F

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

S

G

-

P

G

G

M

M

P

P

E

E

M

M

F

L

Y

T

T

P

T

T

E

S

A

A

I

I

A

M

S

G

D

-

H

A

E

G

L

L

V

G

D

K

S

E

T

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

A

G

T

S

R

H

G

G

P

F

A

V

I

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

S

Q

E

E

G

G

G

G

P

P

I

I

A

G

F

L

V

I

Q

K

E

N

G

G

D

D

L

I

I

I

K

T

I

I

D

D

I

I

P

G

A

A

R

A

K

R

L

I

D

D

I

-

I

-

G

-

T

-

N

-

G

-

I

T

E

Q

K

V

S

S

E

P

K

E

E

E

I

-

E

-

N

-

I

-

L

M

K

N

E

D

R

R

S

R

Q

K

N

K

W

W

V

T

K

A

P

P

A

A

P

Y

R

K

Y

V

T

N

K

R

G

G

I

V

L

L

G

Y

L

K

Y

Y

T

I

R

K

C

N

A

V

S

Q

S

S

P

A

M

S

K

D

G

G

binding fe2/s2 (inorganic) cluster: C62 (≠ G45), D94 (= D77), A95 (≠ G78), C135 (= C118), D136 (= D119), C207 (= C187)
binding magnesium ion: D136 (= D119), K137 (= K120), N285 (= N265), H289 (= H269)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
33% identity, 96% coverage: 20:568/572 of query aligns to 34:518/523 of 8imuA

query
sites
8imuA

G

G

S

V

G

G

W

L

K

S

S

D

E

D

E

D

L

L

N

L

K

K

A

P

Q

Q

I

I

I

G

I

I

E

S

S

S

S

V

F

W

G

Y

H

E

S

G

H
x
N

P

T

G
x
C

S

N

A

M

H

H

L

L

D

L

T

K

L

L

V

S

D

E

E

A

A

V

F

K

K

E

S

G

I

V

D

E

D

N

N

A

G

G

G

M

R

V

G

G

A

F

K

R

Y

F

F

N

V

T

T

I

D

G

I

V

C

S

D

D

G

A

E

I

T

S

Q

M

G

G

H

T

D

R

G

G

M

M

N

C

Y

F

S

S

L

L

A

Q

S

S

R

R

D

D

I

L

M

I

T

A

N

D

L

S

M

I

E

E

I

T

H

V

V

M

Q

S

A

A

T

Q

P

W

F

Y

D

D

A

G

G

N

I

I

F

S

I

I

A

P

S

G

C
|
C

D
|
D

K

K

A

N

V

M

P

P

A

G

H

T

L

I

M

M

A

A

I

M

A

G

R

R

L

L

D

N

M

R

P

P

S

G

I

I

L

M

V

V

P

Y

G

G

G

G

I

T

M

I

N

K

A

P

G

G

P

-

N

-

M

-

L

-

T

-

L

-

E

-

Q

-

I

-

G

G

T

M

Y

Y

N

-

A

-

Q

-

Y

-

E

-

R

-

G

-

E

-

I

-

T

-

K

-

E

-

Q

-

Y

-

E

-

H

-

Y

-

K

-

Q

-

N

-

A

-

C

-

P

-

S

-

C

-

G

-

A

-

C

-

S

-

F

-

M

-

G

-

T

T

A

A

S

N

T

T

M

M

Q

A

V

S

M

A

S

I

E

E

A

A

L

M

G

G

I

M

A

S

L

L

P

P

G

Y

T

S

A

S

L

S

I

I

P

P

V

A

T

-

S

E

K

D

E

P

L

L

K

K

L

L

-

D

A

E

A

C

K

R

N

L

A

A

G

G

K

K

Q

Y

I

L

L

L

K

E

L

L

I

L

E

K

L

M

N

D

I

L

K

K

P

P

S

R

E

D

I

I

M

I

T

T

K

P

K

K

A

S

F

L

E

R

N

N

A

A

I

M

M

V

V

S

H

V

A

M

A

A

I

L

A

G

G

G

S

S

S

T

N

N

C

A

L

V

L

L

H

H

I

L

P

I

A

A

I

I

A

A

H

R

E

S

L

V

G

G

M

L

D

E

I

L

E

T

P

L

E

D

L

D

F

F

D

Q

E

K

I

V

H

S

K

D

K

A

I

V

P

P

Y

F

I

L

L

A

N

D

I

L

R

K

P

P

S

S

G

G

F

K

Y

Y

P

V

G

M

S

E

Y

D

F

I

W

H

N

K

A

I

G

G

G

G

V

T

P

P

A

A

I

V

M

L

E

R

E

Y

I

L

K

L

E

E

-

L

-

G

F

L

L

M

H

D

L

G

D

D

V

C

M

M

T

T

V

V

T

T

G

G

K

Q

T

T

L

L

G

A

E

Q

N

N

L

L

E

E

D

N

L

-

K

-

N

-

S

-

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

V

T

P

S

S

L

L

G

-

I

T

K

E

K

G

E

Q

D

E

V

I

I

I

R

R

T

P

K

L

E

S

N

N

P

P

I

I

Q

K

S

E

Q

T

G

G

A

H

I

I

A

Q

I

I

L

L

K

R

G

G

N

D

L

L

A

A

P

P

G

D

G

G

A

S

V

V

V

A

K

K

H

I

S

T

A

G

I

-

S

-

K

K

K

E

L

G

M

L

Q

Y

V

F

V

S

L

G

K

P

A

A

R

L

V

V

F

F

N

E

C

G

E

E

E

E

D

S

A

M

I

L

K

A

S

A

V

I

L

S

T

A

K

D

N

P

I

M

K

S

-

F

P

K

G

G

D

T

A

V

V

V

F

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

S

G

-

P

G

G

M

M

P

P

E

E

M

M

F

L

Y

T

T

P

T

T

E

S

A

A

I

I

A

M

S

G

D

-

H

A

E

G

L

L

V

G

D

K

S

E

T

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

A

G

T

S

R

H

G

G

P

F

A

V

I

F

G

G

H

H

V

I

S

C

P

P

E

E

A

A

S

Q

E

E

G

G

G

G

P

P

I

I

A

G

F

L

V

I

Q

K

E

N

G

G

D

D

L

I

I

I

K

T

I

I

D

D

I

I

P

G

A

A

R

A

K

R

L

I

D

D

I

-

I

-

G

-

T

-

N

-

G

-

I

T

E

Q

K

V

S

S

E

P

K

E

E

E

I

-

E

-

N

-

I

-

L

M

K

N

E

D

R

R

S

R

Q

K

N

K

W

W

V

T

K

A

P

P

A

A

P

Y

R

K

Y

V

T

N

K

R

G

G

I

V

L

L

G

Y

L

K

Y

Y

T

I

R

K

C

N

A

V

S

Q

S

S

P

A

M

S

K

D

G

G

binding fe2/s2 (inorganic) cluster: N57 (≠ H43), C59 (≠ G45), C132 (= C118), D133 (= D119)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
35% identity, 81% coverage: 89:550/572 of query aligns to 98:546/579 of B5ZZ34

query
sites
B5ZZ34

S

A

L

M

A

M

S

Y

R

R

D

N

I

L

M

A

T

A

N

L

L

A

M

V

E

E

I

E

H

A

V

I

Q

R

A

G

T

Q

P

P

F

M

D

D

A

G

G

C

I

V

F

L

I

L

A

V

S

G

C
|
C

D
|
D

K

K

A

T

V

T

P

P

A

S

H

L

L

L

M

M

A

G

I

A

A

A

R

S

L

C

D

D

M

L

P

P

S

S

I

I

L

V

V

V

P

T

G

G

G

G

I

P

M

M

N

L

A

N

G

G

P

-

N

-

M

Y

L

F

T

R

L

G

E

E

Q

R

I

V

G

G

T

S

-

G

-

T

-

H

-

L

-

W

-

K

Y

F

N

S

A

E

Q

M

Y

V

E

K

R

A

G

G

E

E

I

M

T

T

K

Q

E

A

Q

E

Y

F

E

L

H

E

Y

A

K

E

Q

A

N

S

A

M

C

S

P

R

S

S

C

S

G

G

A

T

C
|
C

S

N

F

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

Q

A

V

S

M

M

S

A

E

E

A

A

L

L

G

G

I

M

A

A

L

L

P

S

G

G

T

N

A

A

L

A

I

I

P

P

V

G

T

V

S

D

K

S

E

R

L

R

K

K

L

V

A

M

A

A

K

Q

N

L

A

T

G

G

K

R

Q

R

I

I

L

V

K

Q

L

M

I

V

E

K

L

D

N

D

I

L

K

K

P

P

S

S

E

E

I

I

M

M

T

T

K

K

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

M

R

V

T

H

N

A

A

A

A

I

I

A

G

G

G

S

S

S

T

N

N

C

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

E

R

L

V

G

G

M

I

D

D

I

L

E

S

P

L

E

D

L

D

F

W

D

D

E

R

I

C

H

G

K

R

K

D

I

V

P

P

Y

T

I

I

L

V

N

N

I

L

R

M

P

P

S

S

G

G

F

K

Y

Y

P

L

G

M

S

E

Y

E

F

F

W

F

N

Y

A

A

G

G

G

G

V

L

P

P

A

V

I

V

M

L

E

K

E

R

I

L

K

G

E

E

-

A

-

G

F

L

L

L

H

H

L

K

D

D

V

A

M

L

T

T

V

V

T

S

G

G

K

E

T

T

L

V

G

W

E

D

N

E

L

V

E

K

D

D

L

V

K

V

N

N

S

W

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

-

T

-

S

-

L

-

G

-

I

-

K

-

K

N

E

E

D

D

V

V

I

I

R

L

T

P

K

A

E

E

N

K

P

A

I

L

Q

T

S

S

Q

S

G

G

A

G

I

I

A

V

I

V

L

L

K

R

G

G

N

N

L

L

A

A

P

P

G

K

G

G

A

A

V

V

V

L

K

K

H

P

S

S

A

A

I

A

S

S

K

P

K

H

L

L

M

L

Q

V

V

H

V

K

L

G

K

R

A

A

R

V

V

V

F

F

N

E

C

D

E

I

E

D

D

D

A

Y

I

K

K

A

S

K

V

I

L

N

T

D

K

D

N

N

-

L

-

D

I

I

K

D

P

E

G

N

D
x
C

A

I

V

M

F

V

V

M

R

K

Y

N

E
x
C

G

G

P

P

K

K

G

G

-

Y

S

P

G

G

M

M

P

A

E
|
E

M

V

F

G

Y

N

T

M

T

G

E

L

A

P

I

P

A

K

S

V

D

L

H

K

E

K

L

G

V

I

D

L

S

D

T

M

A

V

L

R

I

I

T

S

D

D

G

A

R

R

F

M

S
|
S

G

G

A

T

T

A

R

Y

G

G

P

T

A

V

I

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

S

A

E

V

G

G

G

G

P

P

I

L

A

A

F

V

V

V

Q

K

E

N

G

G

D

D

L

M

I

I

K

E

I

L

D

D

I

V

P

P

A

N

R

R

K

R

L

L

D

H

I

-

I

-

G

-

T

-

N

-

G

-

I

L

E

D

K

I

S

S

E

D

K

E

E

E

I

L

E

A

N

R

I

R

L

L

K

A

E

E

R

W

S

Q

Q

P

N

N

W

H

V

D

K

L

P

P

A

T

P

S

R

G

Y

Y

C127 (= C118) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D119) binding Mg(2+)
C200 (= C187) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (≠ D435) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ E442) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E451) binding Mg(2+)
S480 (= S478) mutation to A: Loss of activity.

Sites not aligning to the query:

59 binding [2Fe-2S] cluster; C→S: Loss of activity. Does not bind iron-sulfur cluster.
91 binding Mg(2+)

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
34% identity, 81% coverage: 89:550/572 of query aligns to 95:543/576 of 5j85A

query
sites
5j85A

S

A

L

M

A

M

S

Y

R

R

D

N

I

L

M

A

T

A

N

L

L

A

M

V

E

E

I

E

H

A

V

I

Q

R

A

G

T

Q

P

P

F

M

D

D

A

G

G

C

I

V

F

L

I

L

A

V

S

G

C
|
C

D
|
D

K
|
K

A

T

V

T

P

P

A

S

H

L

L

L

M

M

A

G

I

A

A

A

R

S

L

C

D

D

M

L

P

P

S

S

I

I

L

V

V

V

P

T

G

G

G

G

I

P

M

M

N

L

A

N

G

G

P

-

N

-

M

Y

L

F

T

R

L

G

E

E

Q

R

I

V

G

G

T

S

-

G

-

T

-

H

-

L

-

W

-

K

Y

F

N

S

A

E

Q

M

Y

V

E

K

R

A

G

G

E

E

I

M

T

T

K

Q

E

A

Q

E

Y

F

E

L

H

E

Y

A

K

E

Q

A

N

S

A

M

C

S

P

R

S

S

C

S

G

G

A
x
T

C
|
C

S

N

F

T

M

M

G

G

T
|
T

A

A

S

S

T

T

M

M

Q

A

V

S

M

M

S

A

E

E

A

A

L

L

G

G

I

M

A

A

L

L

P

S

G

G

T

N

A

A

L

A

I

I

P

P

V

G

T

V

S

D

K

S

E

R

L

R

K

K

L

V

A

M

A

A

K

Q

N

L

A

T

G

G

K

R

Q

R

I

I

L

V

K

Q

L

M

I

V

E

K

L

D

N

D

I

L

K

K

P

P

S

S

E

E

I

I

M

M

T

T

K

K

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

M

R

V

T

H

N

A

A

A

A

I

I

A

G

G

G

S

S

S

T

N

N

C

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

E

R

L

V

G

G

M

I

D

D

I

L

E

S

P

L

E

D

L

D

F

W

D

D

E

R

I

C

H

G

K

R

K

D

I

V

P

P

Y

T

I

I

L

V

N

N

I

L

R

M

P

P

S

S

G

G

F

K

Y

Y

P

L

G

M

S

E

Y

E

F

F

W

F

N

Y

A

A

G

G

G

G

V

L

P

P

A

V

I

V

M

L

E

K

E

R

I

L

K

G

E

E

-

A

-

G

F

L

L

L

H

H

L

K

D

D

V

A

M

L

T

T

V

V

T

S

G

G

K

E

T

T

L

V

G

W

E

D

N

E

L

V

E

K

D

D

L

V

K

V

N

N

S

W

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

-

T

-

S

-

L

-

G

-

I

-

K

-

K

N

E

E

D

D

V

V

I

I

R

L

T

P

K

A

E

E

N

K

P

A

I

L

Q

T

S

S

Q

S

G

G

A

G

I

I

A

V

I

V

L

L

K

R

G

G

N

N

L

L

A

A

P

P

G

K

G

G

A

A

V

V

V

L

K

K

H

P

S

S

A

A

I

A

S

S

K

P

K

H

L

L

M

L

Q

V

V

H

V

K

L

G

K

R

A

A

R

V

V

V

F

F

N

E

C

D

E

I

E

D

D

D

A

Y

I

K

K

A

S

K

V

I

L

N

T

D

K

D

N

N

-

L

-

D

I

I

K

D

P

E

G

N

D

C

A

I

V

M

F

V

V

M

R

K

Y

N

E

C

G

G

P

P

K

K

G

G

-

Y

S

P

G

G

M

M

P

A

E
|
E

M

V

F

G

Y

N

T

M

T

G

E

L

A

P

I

P

A

K

S

V

D

L

H

K

E

K

L

G

V

I

D

L

S

D

T

M

A

V

L

R

I

I

T

S

D

D

G

A

R

R

F

M

S

A

G

G

A

T

T

A

R

Y

G

G

P

T

A

V

I

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

S

A

E

V

G

G

G

G

P

P

I

L

A

A

F

V

V

V

Q

K

E

N

G

G

D

D

L

M

I

I

K

E

I

L

D

D

I

V

P

P

A

N

R

R

K

R

L

L

D

H

I

-

I

-

G

-

T

-

N

-

G

-

I

L

E

D

K

I

S

S

E

D

K

E

E

E

I

L

E

A

N

R

I

R

L

L

K

A

E

E

R

W

S

Q

Q

P

N

N

W

H

V

D

K

L

P

P

A

T

P

S

R

G

Y

Y

binding fe2/s2 (inorganic) cluster: C124 (= C118), D125 (= D119), T196 (≠ A186), C197 (= C187)
binding magnesium ion: D125 (= D119), K126 (= K120), T202 (= T192), E450 (= E451)

Sites not aligning to the query:

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg
31% identity, 92% coverage: 33:559/572 of query aligns to 38:540/568 of 8ej0A

query
sites
8ej0A

I

I

I

G

I

I

E

C

S

N

S

T

F

F

G

S

H

E

S

L

H

T

P

P

G
x
C

S

N

A

S

H

H

L

F

D

R

T

T

L

L

V

A

D

E

E

Q

A

V

F

K

K

I

S

G

I

V

D

W

D

E

N

S

G

G

G

G

R

F

G

P

A

L

K

E

Y

F

F

P

V

V

T

M

D

S

I

L

C

G

D
x
E

G

T

E

M

T

L

Q

R

G

P

H

T

D

A

G

M

M

L

N

F

Y

R

S

N

L

L

A

A

S

S

R

M

D

D

I

V

M

-

T

-

N

-

L

-

M

-

E

E

I

E

H

S

V

I

Q

R

A

G

T

N

P

P

F

L

D

D

A

G

G

V

I

V

F

L

I

L

A

M

S

G

C
|
C

D
|
D

K
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binding bicarbonate ion: D119 (= D119), W161 (≠ G157), T196 (= T192), E442 (= E451), S469 (= S478), S469 (= S478), G470 (= G479)
binding fe2/s2 (inorganic) cluster: C50 (≠ G45), E82 (≠ D77), C118 (= C118), D119 (= D119), C191 (= C187)
binding magnesium ion: E82 (≠ D77), D119 (= D119), K120 (= K120), E442 (= E451)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
31% identity, 92% coverage: 33:559/572 of query aligns to 39:541/569 of 8epzA

query
sites
8epzA

I

I

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binding fe2/s2 (inorganic) cluster: C51 (≠ G45), C119 (= C118), D120 (= D119), C192 (= C187)
binding manganese (ii) ion: E83 (≠ D77), D120 (= D119), K121 (= K120), E443 (= E451)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
33% identity, 87% coverage: 22:519/572 of query aligns to 32:519/583 of Q1JUQ1

query
sites
Q1JUQ1

G

G

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G

-

G

-

L

F

L

L

P

H

N

L

P

D

D

V

A

M

L

T

T

V

V

T

N

G

G

K

K

T

S

L

L

G

W

E

D

N

N

L

V

E

R

D

E

L

A

K

P

N

N

S

-

G

-

Y

-

Y

Y

E

D

E

-

H

-

D

-

K

-

L

-

I

-

T

-

S

-

L

-

G

-

I

-

K

-

K

-

E

E

D

E

V

V

I

I

R

R

T

P

K

L

E

D

N

R

P

P

I

L

Q

I

S

A

Q

D

G

G

A

G

I

I

A

R

I

I

L

L

K

R

G

G

N

N

L

L

A

A

P

P

G

R

G

G

A

A

V

V

V

L

K

K

H

P

S

S

A

A

I

A

S

S

K

P

K

E

L

L

M

L

Q

K

V

H

V

R

L

G

K

R

A

A

R

V

V

V

F

F

N

E

C

-

E

N

E

L

D

D

A

H

I

Y

K

K

S

A

V

T

L

I

T

N

K

D

-

E

-

A

-

L

N

D

I

I

K

D

P

A

G

S

D

S

A

V

V

M

F

V

V

L

R

K

Y

N

E
x
C

G

G

P

P

K

R

G

G

-

Y

S

P

G

G

M

M

P

A

E

E

M

V

F

G

Y

N

T

M

T

G

E

L

A

P

I

P

A

K

S

L

D

L

H

R

E

Q

L

G

V

V

D

K

S

D

T

M

A

V

L

R

I

I

T

S

D

D

G

A

R

R

F

M

S

S

G

G

A

T

T

A

R

Y

G

G

P

T

A

V

I

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

S

A

E

A

G

G

G

G

P

P

I

L

A

A

F

A

V

V

Q

R

E

N

G

G

D

D

L

W

I

I

K

E

I

L

D

D

I

C

P

E

A

A

R

G

K

T

L

L

D

H

I

L

C47 (≠ E36) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (≠ G45) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C118) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C187) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ E442) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
30% identity, 87% coverage: 22:520/572 of query aligns to 28:526/587 of 7m3kA

query
sites
7m3kA

G

G

W

L

K

S

S

Q

E

E

E

E

L

L

N

Q

K

S

A

G

Q

R

I

P

I

I

I

I

-

G

-

I

E

A

S

Q

S

T

F

G

G

S

H

D

S

L

H

S

P

P

G
x
C

S

N

A

R

H

H

L

H

D

L

T

E

L

L

V

A

D

K

E

R

A

V

F

R

K

D

S

G

I

V

D

R

D

E

N

A

G

G

G

G

R

I

G

V

A

I

K

E

Y

F

F

P

V

V

T

H

D

P

I

I

C

Q

D

E

G

T

E

G

T

K

Q

R

G

P

H

T

D

A

G

G

M

L

N

D

Y

R

S

N

L

L

A

A

S

-

R

-

D

-

I

-

M

Y

T

L

N

G

L

L

M

V

E

E

I

V

H

-

V

L

Q

Y

A

G

T

Y

P

P

F

L

D

D

A

G

G

V

I

V

F

L

I

T

A

I

S

G

C
|
C

D

D

K

K

A

T

V

T

P

P

A

A

H

C

L

L

M

M

A

A

I

A

A

A

R

T

L

V

D

N

M

I

P

P

S

A

I

I

L

A

V

L

P

S

G

V

G

G

I

P

M

M

N

L

A

N

G

G

P

-

N

-

M

W

L

F

T

R

L

G

E

E

Q

R

I

T

G

G

T

S

Y

G

N

T

A

I

Q

V

Y

W

E

K

-

A

-

R

-

E

-

L

-

L

-

A

R

K

G

G

E

E

I

I

T

D

K

Y

E

Q

Q

G

Y

F

E

V

H

K

Y

L

K

V

Q

A

N

S

A

S

C

A

P

P

S

S

C

T

G

G

A

Y

C

C

S

N

F

T

M

M

G

G

T

T

A

A

S

T

T

T

M

M

Q

N

V

S

M

L

S

A

E

E

A

A

L

L

G

G

I

M

A

Q

L

L

P

P

G

G

T

S

A

A

L

A

I

I

P

P

V

A

T

P

S

Y

K

R

E

D

L

R

K

Q

L

E

A

V

A

A

K

Y

N

L

A

M

G

G

K

R

Q

R

I

I

L

V

K

E

L

M

I

V

E

H

L

E

N

D

I

L

K

K

P

P

S

S

E

D

I

I

M

L

T

T

K

K

K

E

A

A

F

F

E

I

N

N

A

A

I

I

M

R

V

V

H

N

A

S

A

A

I

I

A

G

G

G

S

S

S

T

N

N

C

A

L

P

L

I

H

H

I

L

P

N

A

A

I

L

A

A

H

R

E

H

L

I

G

G

M

V

D

E

I

L

E

T

P

V

E

D

L

D

F

W

D

Q

E

K

I

Y

H

G

K

E

K

E

I

I

P

P

Y

L

I

L

L

V

N

N

I

L

R

Q

P

P

S

A

G

G

F

E

Y

Y

P

L

G

G

S

E

Y

D

F

Y

W

Y

N

H

A

A

G

G

G

G

V

V

P

P

A

A

I

V

M

V

E

N

E

Q

I

L

-

M

-

G

K

Q

E

G

F

L

L

I

H

H

L

E

D

D

V

A

M

I

T

T

V

V

T

N

G

G

K

K

T

T

L

I

G

G

E

E

N

N

L

C

E

K

D

N

L

A

K

T

N

-

S

-

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

-

T

-

S

-

L

-

G

-

I

I

K

E

K

D

E

G

D

N

V

V

I

I

R

K

T

T

K

Y

E

D

N

Q

P

P

I

L

Q

K

S

K

Q

H

G

A

A

G

I

F

A

R

I

V

L

L

K

R

G

G

N

N

L

L

A

F

P

-

G

S

G

S

A

A

V

I

V

M

K

K

H

L

S

S

A

V

I

I

S

S

K

D

K

E

L

F

M

R

Q

N

V

R

V

Y

L

L

-

S

-

D

-

A

-

K

-

D

-

P

-

N

-

A

-

F

-

E

-

G

K

K

A

A

R

V

V

V

F

F

N

D

C

G

E

P

E

E

D

D

A

Y

I

H

K

H

S

R

V

I

-

D

-

D

L

P

T

A

K

L

N

E

I

I

K

D

P

E

G

H

D

T

A

V

V

L

F

F

V

M

R

R

Y

G

E

A

G

G

P

P

K

-

G

-

S

I

G

G

M

Y

P

P

E

G

M

A

F

A

Y

E

T

V

T

V

E

N

A

M

I

R

A

A

S

P

D

D

H

Y

E

L

L

L

-

K

-

K

-

G

V

I

D

T

S

S

T

L

A

P

L

C

I

I

T

G

D

D

G

G

R

R

F

Q

S

S

G

G

A

T

T

S

R

G

G

S

P

P

A

S

I

I

G

L

H

N

V

A

S

S

P

P

E

E

A

A

S

A

E

A

G

G

G

G

P

G

I

L

A

A

F

I

V

L

Q

K

E

T

G

G

D

D

L

R

I

V

K

R

I

I

D

D

I

L

P

G

A

R

R

G

K

T

L

A

D

D

I

I

I

L

binding zinc ion: C53 (≠ G45), C121 (= C118)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see 2 papers)
31% identity, 92% coverage: 48:572/572 of query aligns to 68:578/595 of Q9A9Z2

query
sites
Q9A9Z2

H

H

L

L

D

D

T

-

L

L

V

V

D

Q

E

R

A

V

F

R

K

D

S

G

I

I

D

R

D

D

N

A

G

G

G

G

R

I

G

P

A

M

K

E

Y

F

F

P

V

V

T

H

D

P

I

I

C

F

D
x
E

G

N

E

C

T

R

Q

R

G

P

H

T

D

A

G

A

M

L

N

D

Y

R

S

N

L

L

A

S

S

Y

R

L

D

G

I

L

M

V

T

E

N

T

L

L

M

-

E

-

I

-

H

-

V

-

Q

H

A

G

T

Y

P

P

F

I

D

D

A

A

G

V

I

V

F

L

I

T

A

T

S

G

C
|
C

D
|
D

K

K

A

T

V

T

P

P

A

A

H

G

L

I

M

M

A

A

I

A

A

T

R

T

L

V

D

N

M

I

P

P

S

A

I

I

L

V

V

L

P

S

G

G

G

G

I

P

M

M

N

L

A

D

G

G

P

W

N

H

M

E

L

N

T

E

L

L

E

V

Q

G

I

S

G

G

T

T

Y

V

N

I

A

W

Q

R

Y

S

E

R

R

R

-

K

-

L

-

A

-

A

G

G

E

E

I

I

T

T

K

E

E

E

Q

E

Y

F

E

I

H

D

Y

R

K

A

Q

A

N

S

A

S

C

A

P

P

S

S

C

A

G

G

A

H

C
|
C

S

N

F

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

Q

N

V

A

M

V

S

A

E

E

A

A

L

L

G

G

I

L

A

S

L

L

P

T

G

G

T

C

A

A

L

A

I

I

P

P

V

A

T

P

S

Y

K

R

E

E

L

R

K

G

L

Q

A

M

A

A

K

Y

N

K

A

T

G

G

K

Q

Q

R

I

I

L

V

K

D

L

L

I

A

E

Y

L

D

N

D

I

V

K

K

P

P

S

L

E

D

I

I

M

L

T

T

K

K

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

M

A

V

L

H

V

A

A

A

A

I

A

A

G

G

G

S

S

S

T

N

N

C

A

L

Q

L

P

H

H

I

I

P

V

A

A

I

M

A

A

H

R

E

H

L

A

G

G

M

V

D

E

I

I

E

T

P

A

E

D

L

D

F

W

D

R

E

A

I

A

H

Y

K

-

K

D

I

I

P

P

Y

L

I

I

L

V

N

N

I

M

R

Q

P

P

S

A

G

G

F

K

Y

Y

P

L

G

G

S

E

Y

R

F

F

W

H

N

R

A

A

G

G

G

G

V

A

P

P

A

A

I

V

M

L

E

W

E

E

I

L

K

L

E

Q

-

Q

-

G

F

R

L

L

H

H

L

G

D

D

V

V

M

L

T

T

V

V

T

T

G

G

K

K

T

T

L

M

G

S

E

E

N

N

L

L

E

Q

D

G

L

R

K

E

N

T

S

S

G

-

Y

-

Y

-

E

-

E

-

H

-

D

-

K

-

L

-

I

-

T

-

S

-

L

-

G

-

I

-

K

-

K

D

E

R

D

E

V

V

I

I

R

F

T

P

K

Y

E

H

N

E

P

P

I

L

Q

A

S

E

Q

K

G

A

A

G

I

F

A

L

I

V

L

L

K

K

G

G

N

N

L

L

A

F

P

-

G

D

G

F

A

A

V

I

V

M

K

K

H

S

S

S

A

V

I

I

S

G

K

E

K

E

L

F

M

R

Q

K

V

R

V

Y

L

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

-

F

-

E

-

A

K

R

A

A

R

I

V

V

F

F

N

D

C

G

E

S

E

D

D

D

A

Y

I

H

K

K

S

R

V

I

L

N

T

D

K

P

N

A

I

L

K

E

P

I

G

D

D

E

A

R

V
x
C

F

I

V

L

R

V

Y

I

E

R

G

G

P

A

K

G

G

P

S

I

G

G

M

W

P

P

E

G

M

S

F

A

Y
x
E

T

V

T

V

E

N

A

M

I

Q

A

P

S

P

D

D

H

H

E

L

L

L

-

K

-

K

-

G

V

I

D

M

S

S

T

L

A

P

L

T

I

L

T

G

D

D

G

G

R

R

F

Q

S

S

G

G

A

T

T

A

R

D

G

S

P

P

A

S

I

I

G

L

H

N

V

A

S

S

P

P

E

E

A

S

S

A

E

I

G

G

G

G

P

G

I

L

A

S

F

W

V

L

Q

R

E

T

G

G

D

D

L

T

I

I

K

R

I

I

D

D

I

L

P

N

A

T

R

G

K

R

L

C

D

D

I

A

I

L

G

V

T

D

N

-

G

-

I

-

E

-

K

-

S

-

E

-

K

-

E

-

I

-

E

E

N

A

I

T

L

I

K

A

E

A

R

R

S

K

Q

Q

N

D

W

G

V

I

K

P

P

A

A

V

P

P

R

A

Y

T

T

M

K

T

G

P

I

W

L

Q

G

E

L

I

Y

Y

T

R

R

A

C

H

A

A

S

S

S

Q

P

L

M

D

K

T

G

G

G

G

Y

V

M

L

E

E

E96 (≠ D77) binding Mg(2+)
C132 (= C118) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
D133 (= D119) binding Mg(2+)
C205 (= C187) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
C450 (≠ V437) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E467 (≠ Y454) binding Mg(2+)

Sites not aligning to the query:

64 binding [2Fe-2S] cluster; C→S: Strong decrease in activity. Does not bind iron-sulfur cluster.

5oynA Crystal structure of d-xylonate dehydratase in holo-form (see paper)
31% identity, 92% coverage: 48:572/572 of query aligns to 62:572/589 of 5oynA

query
sites
5oynA

H

H

L

L

D

D

T

-

L

L

V

V

D

Q

E

R

A

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K

D

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x
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x
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E

N

T

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L

M

-

E

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-

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Q

H

A

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I

D

D

A

A

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L

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G

C
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C

D
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D

K
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K

A

T

V

T

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P

A

A

H

G

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M

M

A

A

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A

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M

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S

A

I

I

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V

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P

S

G

G

G

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M

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A

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A

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A

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A

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G

G

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C
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C

S

N

F

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M

M

G

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A

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M

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Q

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L

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P

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L

E

D

I

I

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T

K

K

K

Q

A

A

F

F

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E

N

N

A

A

I

I

M

A

V

L

H

V

A

A

A

A

I

A

A

G

G

G

S

S

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N

N

C

A

L

Q

L

P

H

H

I

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A

A

I

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A

A

H

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H

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A

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A

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A

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-

K

D

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Y

L

I

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N

N

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P

P

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A

G

G

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K

Y

Y

P

L

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G

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A

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A

A

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E

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K

L

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-

Q

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G

F

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L

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H

L

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D

D

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V

M

L

T

T

V

V

T

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G

G

K

K

T

T

L

M

G

S

E

E

N

N

L

L

E

Q

D

G

L

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K

E

N

T

S

S

G

-

Y

-

Y

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A

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x
E

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A

P

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P

D

D

H

H

E

L

L

L

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-

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-

G

V

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L

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L

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G

D

D

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G

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S

G

G

A

T

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A

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D

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P

A

S

I

I

G

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H

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V

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P

P

E

E

A

S

S

A

E

I

G

G

G

G

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G

I

L

A

S

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W

V

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E

T

G

G

D

D

L

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I

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K

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I

I

D

D

I

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P

N

A

T

R

G

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R

L

C

D

D

I

A

I

L

G

V

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D

N

-

G

-

I

-

E

-

K

-

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I

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E

N

A

I

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L

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A

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D

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A

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E

E

binding fe2/s2 (inorganic) cluster: N91 (≠ G78), C126 (= C118), D127 (= D119), C199 (= C187)
binding magnesium ion: E90 (≠ D77), D127 (= D119), K128 (= K120), E461 (≠ Y454)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58

Query Sequence

>WP_010966867.1 NCBI__GCF_000008765.1:WP_010966867.1
MLKSQEIRQKAPELDSLRLGSGWKSEELNKAQIIIESSFGHSHPGSAHLDTLVDEAFKSI
DDNGGRGAKYFVTDICDGETQGHDGMNYSLASRDIMTNLMEIHVQATPFDAGIFIASCDK
AVPAHLMAIARLDMPSILVPGGIMNAGPNMLTLEQIGTYNAQYERGEITKEQYEHYKQNA
CPSCGACSFMGTASTMQVMSEALGIALPGTALIPVTSKELKLAAKNAGKQILKLIELNIK
PSEIMTKKAFENAIMVHAAIAGSSNCLLHIPAIAHELGMDIEPELFDEIHKKIPYILNIR
PSGFYPGSYFWNAGGVPAIMEEIKEFLHLDVMTVTGKTLGENLEDLKNSGYYEEHDKLIT
SLGIKKEDVIRTKENPIQSQGAIAILKGNLAPGGAVVKHSAISKKLMQVVLKARVFNCEE
DAIKSVLTKNIKPGDAVFVRYEGPKGSGMPEMFYTTEAIASDHELVDSTALITDGRFSGA
TRGPAIGHVSPEASEGGPIAFVQEGDLIKIDIPARKLDIIGTNGIEKSEKEIENILKERS
QNWVKPAPRYTKGILGLYTRCASSPMKGGYME

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory