SitesBLAST
Comparing WP_010966883.1 NCBI__GCF_000008765.1:WP_010966883.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
45% identity, 82% coverage: 44:260/264 of query aligns to 8:224/225 of 4zv2A
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
46% identity, 82% coverage: 44:260/264 of query aligns to 8:226/226 of 4zv1A
- binding arginine: E11 (≠ D47), F14 (≠ Y50), F52 (= F89), A69 (≠ S106), G70 (≠ S107), M71 (≠ I108), T72 (≠ S109), R77 (= R114), Q117 (≠ E154), S120 (≠ T157), T121 (= T158), D161 (= D196)
3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
38% identity, 88% coverage: 32:263/264 of query aligns to 5:234/237 of 3vv5A
- binding l-thialysine: E20 (≠ D47), F23 (≠ Y50), N27 (≠ E54), F61 (= F89), A78 (≠ S106), S79 (= S107), G81 (≠ S109), R86 (= R114), T127 (= T157), T128 (= T158), Y129 (≠ A159)
3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
38% identity, 88% coverage: 32:263/264 of query aligns to 9:238/241 of 3vvfA
- binding arginine: E24 (≠ D47), F27 (≠ Y50), F65 (= F89), A82 (≠ S106), S83 (= S107), H84 (≠ I108), G85 (≠ S109), R90 (= R114), Q128 (≠ E154), T131 (= T157), T132 (= T158), Y133 (≠ A159), E196 (= E221)
3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
38% identity, 88% coverage: 32:263/264 of query aligns to 9:238/241 of 3vveA
- binding lysine: E24 (≠ D47), F27 (≠ Y50), F65 (= F89), S83 (= S107), H84 (≠ I108), R90 (= R114), Q128 (≠ E154), T131 (= T157), T132 (= T158), Y133 (≠ A159), E196 (= E221)
3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
38% identity, 88% coverage: 32:263/264 of query aligns to 9:238/241 of 3vvdA
- binding L-ornithine: E24 (≠ D47), F65 (= F89), S83 (= S107), H84 (≠ I108), G85 (≠ S109), R90 (= R114), Q128 (≠ E154), T131 (= T157), T132 (= T158), Y133 (≠ A159), E196 (= E221)
5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
40% identity, 86% coverage: 32:259/264 of query aligns to 2:228/229 of 5t0wA
- binding arginine: D17 (= D47), Y20 (= Y50), W58 (≠ F89), S75 (= S106), G76 (≠ S107), M77 (≠ I108), T78 (≠ S109), R83 (= R114), Q126 (≠ E154), T129 (= T157), T130 (= T158), D168 (= D196)
6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
38% identity, 86% coverage: 32:259/264 of query aligns to 2:227/229 of 6svfA
- binding arginine: F20 (≠ Y50), F58 (= F89), S75 (= S106), G76 (≠ S107), M77 (≠ I108), T78 (≠ S109), R83 (= R114), Q124 (≠ E154), T127 (= T157), T128 (= T158), D165 (= D196)
8eyzA Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
36% identity, 84% coverage: 40:262/264 of query aligns to 3:224/226 of 8eyzA
4ymxA Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
38% identity, 83% coverage: 42:259/264 of query aligns to 3:222/224 of 4ymxA
- binding arginine: S8 (≠ D47), D10 (≠ T49), F11 (≠ Y50), F52 (= F89), A69 (≠ S106), G70 (≠ S107), M71 (≠ I108), T72 (≠ S109), R77 (= R114), Q116 (≠ E154), T119 (= T157), T120 (= T158), E157 (≠ D196)
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
34% identity, 85% coverage: 40:264/264 of query aligns to 26:258/260 of P02910
- E40 (= E54) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (= K56) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (≠ K62) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D184) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R189) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (≠ A191) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
34% identity, 85% coverage: 40:264/264 of query aligns to 4:236/238 of 1hslA
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
35% identity, 85% coverage: 40:264/264 of query aligns to 26:258/260 of P0AEU0
- C60 (≠ A75) modified: Disulfide link with 67
- C67 (≠ T82) modified: Disulfide link with 60
- S91 (= S106) binding L-histidine
- S92 (= S107) binding L-histidine
- S94 (= S109) binding L-histidine
- R99 (= R114) binding L-histidine
- T143 (= T158) binding L-histidine
- D183 (= D196) binding L-histidine
Sites not aligning to the query:
3k4uE Crystal structure of putative binding component of abc transporter from wolinella succinogenes dsm 1740 complexed with lysine
32% identity, 83% coverage: 41:259/264 of query aligns to 5:226/234 of 3k4uE
- binding lysine: E11 (≠ D47), Y14 (= Y50), W52 (≠ F89), G70 (≠ S107), T72 (≠ S109), R77 (= R114), K120 (≠ E154), V123 (≠ T157), S124 (≠ T158), E144 (≠ M178), D162 (= D196)
4ohnA Crystal structure of an abc uptake transporter substrate binding protein from streptococcus pneumoniae with bound histidine
31% identity, 88% coverage: 31:263/264 of query aligns to 4:243/246 of 4ohnA
- binding histidine: D20 (= D47), F23 (≠ Y50), W61 (≠ F89), N78 (≠ S106), G79 (≠ S107), S81 (= S109), R86 (= R114), Q125 (≠ E154), S128 (≠ T157), S129 (≠ T158), D174 (= D196)
2q2cA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
33% identity, 84% coverage: 40:260/264 of query aligns to 1:221/231 of 2q2cA
- binding histidine: F11 (≠ Y50), W48 (≠ F89), S65 (= S106), G66 (≠ S107), I67 (= I108), T68 (≠ S109), R73 (= R114), Q112 (≠ E154), T115 (= T157), T116 (= T158), D155 (= D196)
2pvuA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
33% identity, 84% coverage: 40:260/264 of query aligns to 5:225/235 of 2pvuA
2q2aA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
33% identity, 84% coverage: 40:260/264 of query aligns to 11:231/241 of 2q2aA
- binding arginine: D18 (= D47), F21 (≠ Y50), W58 (≠ F89), S75 (= S106), G76 (≠ S107), I77 (= I108), T78 (≠ S109), R83 (= R114), Q122 (≠ E154), T125 (= T157), T126 (= T158), D165 (= D196)
5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
29% identity, 85% coverage: 40:263/264 of query aligns to 3:253/255 of 5itoA
- binding octopine: E10 (≠ D47), Y13 (= Y50), W51 (≠ F89), A68 (≠ S106), A69 (≠ S107), G71 (≠ S109), R76 (= R114), Q139 (≠ E154), T142 (= T157), S143 (≠ T158), H144 (≠ A159), S181 (= S195)
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
33% identity, 97% coverage: 10:264/264 of query aligns to 4:258/260 of P02911
- D33 (= D47) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (= Y50) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (= D67) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (≠ A75) modified: Disulfide link with 67
- C67 (≠ T82) modified: Disulfide link with 60
- F74 (= F89) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (= S106) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S107) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (= S109) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R114) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (≠ E154) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (= T158) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D196) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
Query Sequence
>WP_010966883.1 NCBI__GCF_000008765.1:WP_010966883.1
MLKKIFSLSVVLVLTLAIFTGCTSATVSKDDSLERVKKAKEIVIGIDDTYPPMEFKDKAT
GKVSGFDIDMANAIAKKLGVKTKFVPNSFDGIFLALKSKKFDVVHSSISITDERKKAMIF
SDPYIYGGNAIFVKSDNTTIKNEKDFNNQVVGCEVGTTAQDVLAKISGIKEVKKYNAMTD
AFLDLQNGRIAAVVSDPMVGDYYNVTDPGKFKKLESSLTQEPIGVAFRKEDKSLRDAYNK
AIKELKKDGTMSKLSKKWFGIDVY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory