SitesBLAST

C26 (≠ R22) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ S23) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L40) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S49) mutation to T: Loss of ATPase activity and transport.
L60 (≠ F55) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V71) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ L134) mutation to M: Loss of ATPase activity and transport.
D172 (= D171) mutation to N: Loss of ATPase activity and transport.
C276 (≠ M275) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E297) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
38% identity, 74% coverage: 20:299/377 of query aligns to 9:284/358 of 8y5iA

query
sites
8y5iA

R

R

K

K

R

C

S
x
F

T

D

G

G

K
|
K

A

E

F

V

T

I

L

P

D

Q

V

L

S

D

F

L

E

T

M

I

D

N

N

N

-

G

E

E

L

F

V

L

V

T

L

L

F

L

G

G

P

P

S

S

G
|
G

S

C

G

G

K
|
K

T
|
T

L

V

F

L

K

R

C

L

I

I

S

A

G

G

I

L

T

E

Q

T

P

V

D

D

N

S

G

G

K

R

I

I

T

M

V

L

G

-

S

-

K

-

I

-

Y

-

D

D

K

N

D

E

K

D

K

I

I

T

N

H

L

V

P

P

I

A

Q

E

K

N

R

R

N

Y

L

V

G

N

Y

T

V

V

F

F

Q

Q

N

S

Y

Y

T

A

L

L

F

F

P

P

H

H

M

M

N

T

V

V

R

F

K

E

N

N

I

V

E

A

C

F

G

G

L

L

K

R

K

M

W

Q

E

K

K

T

E

P

D

A

R

A

E

E

V

I

-

T

-

P

R

R

V

V

M

M

E

E

M

A

L

L

N

R

L

M

L

V

H

Q

I

L

E

E

E

T

L

F

E

A

T

Q

R
|
R

Y

K

P

P

S

H

Q
|
Q

I

L

S
|
S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

I

A

A

R

R

A

A

L

V

A

V

P

N

K

K

P

P

G

R

I

L

L

L

D

D

E

Q

P

S

F

L

S

S

A

A

L

L

D

D

M

Y

E

K

I

L

R

R

T

K

E

Q

L

M

A

Q

D

N

K

E

I

L

K

K

N

A

L

L

Q

Q

K

R

K

K

I

L

E

G

I

I

P

T

L

F

L

V

F

F

I

V

T

T

H

H

N

D

L

Q

E

E

A

A

F

L

L

T

L

M

A

S

D

D

R

R

I

I

L

V

I

V

L

M

H

R

G

D

G

G

K

R

I

I

Q

E

Q

Q

F

D

G

G

T

T

P

P

E

R

E

E

I

I

F

Y

Y

E

Q

E

P

P

A

K

N

N

L

L

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

I

E

S

I

N

N

I

M

F

F

D

N

D

A

A

T

Y

V

V

I

E

E

E

R

Y

L

D

D

K

E

E

Q

S

R

K

V

S

R

T

A

V

N

L

V

R

E

S

G

G

R

D

E

M

C

R

N

I

I

K

Y

I

V

E

-

S

-

P

-

N

N

F

F

-

A

-

V

K

E

A

P

G

G

D

Q

K

K

V

L

T

H

W

V

G

L

I

L

Y

R

P

P

E

E

N

D

I

L

binding adenosine-5'-triphosphate: F12 (≠ S23), K15 (= K26), G38 (= G48), K41 (= K51), T42 (= T52), T43 (= T53), R128 (= R142), Q132 (= Q146), S134 (= S148)

3d31A Modbc from methanosarcina acetivorans (see paper)
39% identity, 73% coverage: 26:301/377 of query aligns to 12:278/348 of 3d31A

query
sites
3d31A

K

K

A

N

F

F

T

S

L

L

D

D

-

N

V

L

S

S

F

L

E

K

M

V

D

E

N

S

-

G

E

E

L

Y

V

F

V

V

L

I

F

L

G

G

P

P

S

T

G

G

S

A

G

G

K

K

T

T

T

L

L

F

F

L

K

E

C

L

I

I

S

A

G

G

I

F

T

H

Q

V

P

P

D

D

N

S

G

G

K

R

I

I

T

L

V

L

G

D

S

G

K

K

I

D

Y

V

Y

T

D

D

K

-

D

-

K

-

I

-

N

-

L

L

P

S

I

P

Q

E

K

K

R

H

N

D

L

I

G

A

Y

F

V

V

F

Y

Q

Q

N

N

Y

Y

T

S

L

L

F

F

P

P

H

H

M

M

N

N

V

V

R

K

K

K

N

N

I

L

E

E

C

F

G

G

L

M

K

R

K

M

W

K

E

K

K

I

E

K

D

D

R

P

E

K

V

-

R

R

V

V

M

L

E

D

M

T

L

A

N

R

L

D

L

L

H

K

I

I

E

E

E

H

L

L

E

L

T

D

R

R

Y

N

P

P

S

L

Q

T

I

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

P

T

K

N

P

P

G

K

I

I

L

L

D

D

E

E

P

P

F

L

S

S

A

A

L

L

D

D

M

P

E

R

I

T

R

Q

T

E

E

N

L

A

A

R

D

E

K

M

I

L

K

S

N

V

L

L

Q

H

K

K

I

N

E

K

I

L

P

T

L

V

L

L

F

H

I

I

T

T

H

H

N

D

L

Q

E

T

E

E

A

A

F

R

L

I

L

M

A

A

D

D

R

R

I

I

L

A

I

V

L

V

H

M

G

D

G

G

K

K

I

L

Q

I

Q

Q

F

V

G

G

T

K

P

P

E

E

I

I

F

F

Y

E

Q

K

P

P

A

V

N

E

L

G

Q

R

V

V

S

A

E

S

L

F

I

V

G

G

I

F

S

E

N

N

I

V

F

L

D

K

D

G

A

R

Y

V

V

I

E

-

Y

-

D

S

K

A

E

E

S

Q

K

G

S

L

T

L

V

R

L

I

R

R

S

V

G

G

D

E

M

V

R

V

I

I

K

D

I

-

E

A

S

A

P

G

N

D

F

M

K

E

A

V

G

G

D

D

K

Q

V

V

T

Y

W

A

G

F

I

L

Y

R

P

P

E

E

N

N

I

I

T

A

L

L

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
38% identity, 70% coverage: 37:301/377 of query aligns to 31:280/353 of 1vciA

query
sites
1vciA

D

D

N

G

E

E

L

F

V

L

V

V

L

L

F

L

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
|
T

L

T

F

L

K

R

C

M

I

I

S

A

G

G

I

L

T

E

Q

E

P

P

D

T

N

E

G

G

K

R

I

I

T

Y

V

F

G

G

S

-

K

-

I

-

Y

-

D

D

K

R

D

D

K

V

K

T

I

Y

N

-

L

L

P

P

I

P

Q

K

K

D

R

R

N

N

L

I

G

S

Y

M

V

V

F

F

Q

Q

N

-

Y

-

T

-

L

-

F

-

P

-

H

H

M

M

N

T

V

V

R

Y

K

E

N

N

I

I

E

A

C

F

G

P

L

L

K

K

W

F

E

P

K

K

E

D

D

E

R

I

E

D

V

K

R

R

V

V

M

R

E

W

M

A

L

A

N

E

L

L

H

Q

I

I

E

E

L

L

E

L

T

N

R

R

Y

Y

P

P

S

A

Q

Q

I

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

A

A

L

I

A

V

P

V

K

E

P

P

G

D

I

V

L

L

L

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

K

I

L

R

R

T

V

E

A

L

M

A

R

D

A

K

E

I

I

K

K

N

K

L

L

Q

Q

K

Q

K

K

I

L

E

K

I

V

P

T

L

T

L

I

F

Y

I

V

T

T

H

H

N

D

L

Q

E

V

E

E

A

A

F

M

L

T

L

M

A

G

D

D

R

R

I

I

L

A

I

V

L

M

H

N

G

R

G

G

K

Q

I

L

Q

L

Q

Q

F

I

G

G

T

S

P

P

E

T

E

E

I

V

F

Y

Y

L

Q

R

P

P

A

N

N

S

L

V

Q

F

V

V

S

A

E

T

L

F

I

I

G

G

I

A

S

P

-

E

-

M

N

N

I

I

F

L

D

E

D

V

A

S

Y

V

V

G

E

D

E

G

Y

Y

D

-

K

-

E

-

S

-

K

-

S

-

T

-

V

-

L

L

R

E

S

G

G

R

D

G

M

F

R

R

I

I

K

E

I

L

E

P

S

Q

P

M

N

D

F

L

-

L

-

K

-

D

K

Y

A

V

G

G

D

K

K

T

V

V

T

L

W

F

G

G

I

I

Y

R

P

P

E

E

N

H

I

M

T

T

L

V

binding adenosine-5'-triphosphate: S41 (= S47), G42 (= G48), G44 (= G50), K45 (= K51), T46 (= T52), T47 (= T53)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
37% identity, 83% coverage: 31:343/377 of query aligns to 21:319/350 of 3fvqB

query
sites
3fvqB

D

D

V

I

S

S

F

L

E

S

M

L

D

D

-

P

N

G

E

E

L

I

V

L

V

F

L

I

F

I

G

G

P

A

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

L

F

L

K

R

C

C

I

L

S

A

G

G

I

F

T

E

Q

Q

P

P

D

D

N

S

G

G

K

E

I

I

T

S

V

L

G

S

S

G

K

K

I

T

Y

I

Y

F

D

S

K

K

D

N

K

T

K

-

I

-

N

N

L

L

P

P

I

V

Q

R

K

E

R

R

N

R

L

L

G

G

Y

Y

V

L

F

V

Q
|
Q

N

E

Y

G

T

V

L

L

F

F

P

P

H

H

M

L

N

T

V

V

R

Y

K

R

N

N

I

I

E

A

C

Y

G

G

L

L

-

G

-

N

-

G

K

K

K

G

W

R

E

T

K

A

E

Q

D

E

R

R

E

Q

V

-

R

R

V

I

M

E

E

A

M

M

L

L

N

E

L

L

L

T

H

G

I

I

E

S

E

E

L

L

E

A

T

G

R
|
R

Y

Y

P

P

S

H

Q
x
E

I

L

S
|
S

G

G

G
|
G

Q
|
Q

K

Q

Q

Q

R

R

V

A

A

A

L

L

A

A

R

R

A

A

L

L

A

A

P

P

K

D

P

P

G

E

I

L

L

I

L

L

D

D

E

E

P

P

F

F

S

S

A

A

L

L

D

D

M

E

E

Q

I

L

R

R

T

R

E

Q

L

I

A

R

D

E

K

D

I

M

K

I

N

A

L

A

Q

L

K

R

K

A

I

N

E

G

I

K

P

S

L

A

L

V

F

F

I

V

T

S

H

H

N

D

L

R

E

E

A

A

F

L

L

Q

L

Y

A

A

D

D

R

R

I

I

L

A

I

V

L

M

H

K

G

Q

G

G

K

R

I

I

Q

L

Q

Q

F

T

G

A

T

S

P

P

E

H

E

E

I

L

F

Y

Y

R

Q

Q

P

P

A

A

N

D

L

L

Q

D

V

A

S

A

E

L

L

F

I

I

G

G

I

E

S

G

N

I

I

V

F

F

D

P

D

A

A

A

Y

L

V

N

E

A

E

D

Y

G

D

T

K

A

E

D

S

C

K

-

S

-

T

-

V

-

L

-

R

R

S

L

G

G

D

R

M

L

R

P

I

V

K

Q

I

S

E

G

S

A

P

P

N

-

F

-

K

-

A

A

G

G

D

T

K

R

V

G

T

T

W

L

G

L

I

I

Y

R

P

P

E

E

N

Q

I

Y

T

S

L

L

L

H

P

P

V

H

S

S

G

A

S

P

E

-

D

-

Q

-

D

-

E

-

N

-

I

-

Y

-

S

A

A

A

H

S

V

I

N

H

N

A

I

V

N

L

K

K

-

T

G

T

P

P

K

K

K

A

R

R

I

H

T

T

L

E

K

I

L

S

V

L

R

R

Y

A

N

G

K

Q

T

T

L

V

I

L

binding adenosine-5'-triphosphate: S38 (= S47), G39 (= G48), C40 (≠ S49), G41 (= G50), K42 (= K51), T43 (= T52), T44 (= T53), R133 (= R142), E137 (≠ Q146), S139 (= S148), G141 (= G150), Q142 (= Q151)
binding calcium ion: T43 (= T52), Q86 (= Q97)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 14, 16, 18

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 61% coverage: 18:248/377 of query aligns to 9:236/393 of P9WQI3

query
sites
P9WQI3

I

V

N

N

R

K

K

S

R

Y

S

P

T

D

G

G

K

H

A

T

F

A

T

V

L

R

D

D

V

L

S

N

F

L

E

T

M

I

-

A

D

D

N

G

E

E

L

F

V

L

V

I

L

L

F

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

L

T

F

L

K

N

C

M

I

I

S

A

G

G

I

L

T

E

Q

D

P

I

D

S

N

S

G

G

K

E

I

L

T

R

V

I

G

A

S

G

K

E

I

R

Y

V

Y

N

D

E

K

K

D

A

K

P

K

K

I

-

N

-

L

-

P

-

I

-

Q

-

K

D

R

R

N

D

L

I

G

A

Y

M

V

V

F

F

Q

Q

N

S

Y

Y

T

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

R

R

K

Q

N

N

I

I

E

A

-

F

-

P

C

L

G

T

L

L

K

A

K

K

W

M

E

R

K

K

E

A

D

D

R

I

E

A

V

Q

R

K

V

V

M

S

E

E

M

T

L

A

N

K

L

I

L

L

H

D

I

L

E

T

E

N

L

L

E

L

T

D

R

R

Y

K

P

P

S

S

Q

Q

I

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

K

H

P

P

G

K

I

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

K

I

L

R

R

T

V

E

Q

L

M

A

R

D

G

K

E

I

I

K

A

N

Q

L

L

Q

Q

K

R

I

L

E

G

I

T

P

T

L

T

L

V

F

Y

I

V

T

T

H
|
H

N

D

L

Q

E

T

E

E

A

A

F

M

L

T

L

L

A

G

D

D

R

R

I

V

L

V

I

V

L

M

H

Y

G

G

K

I

I

A

Q

Q

F

I

G

G

T

T

P

P

E

E

I

L

F

Y

Y

E

Q

R

P

P

A

A

N

N

L

L

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

H193 (= H205) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
38% identity, 67% coverage: 21:271/377 of query aligns to 34:289/382 of 7ahhC

query
sites
7ahhC

K

K

R

K

S

T

T

G

G

A

K
x
T

A
x
V

F
x
G

T

V

L

Y

D

D

V

T

S

N

F

F

E

E

M

I

-

N

D

E

N

G

E

E

L

I

V

F

V

V

L

I

F

M

G

G

P

L

S

S

G
|
G

S

S

G
|
G

K
|
K

T

S

T

T

L

L

F

L

K

R

C

L

I

L

S

N

G

R

I

L

T

I

Q

E

P

P

D

T

N

S

G

G

K

K

I

I

T

F

V

I

G

D

S

N

K

Q

I

D

Y

V

Y

A

D

T

K

L

D

N

K

K

K

E

I

D

N

L

L

L

P

Q

I

V

Q

R

K

R

R

K

N

T

L

M

G

S

Y

M

V

V

F

F

Q

Q

N

N

Y

F

T

G

L

L

F

F

P

P

H

H

M

R

N

T

V

I

R

L

K

E

N

N

I

T

E

E

C

Y

G

G

L

L

-

E

-

V

K

Q

K

N

W

V

E

P

K

K

E

E

D

E

R

R

E

R

V

K

R

R

V

A

M

E

E

K

M

A

L

L

N

D

L

N

L

A

H

N

I

L

E

L

E

D

L

F

E

K

T

D

R

Q

Y

Y

P

P

S

K

Q

Q

I

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

P

N

K

D

P

P

G

E

I

I

L

L

L

M

D
|
D

E
|
E

P

A

F

F

S

S

A

A

L

L

D

D

M

P

E

L

I

I

R

R

T

R

E

E

L

M

A

Q

D

D

K

E

I

L

K

L

N

E

L

L

Q

Q

K

A

K

K

I

F

E

Q

I

K

P

T

L

I

F

F

I

V

T

S

H

H

N

D

L

L

E

N

E

E

A

A

F

L

L

R

L

I

A

G

D

D

R

R

I

I

L

A

I

I

L

M

H

K

G

D

G

G

K

K

I

I

Q

M

Q

Q

F

I

G

G

T

T

P

G

E

E

I

I

F

L

Y

T

Q

N

P

P

A

A

N

N

L

D

Q

Y

V

V

S

K

E

T

L

F

I

V

-

E

-

D

G

T

I

A

S

E

N

N

I

I

F

M

D

I

D

P

A

A

Y
x
L

V

T

E

T

E

N

Y

I

D

D

K

V

E

D

S

G

K

P

S

S

T

V

V

A

L

L

R

K

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ Y257)
binding phosphoaminophosphonic acid-adenylate ester: T39 (≠ K26), V40 (≠ A27), G41 (≠ F28), G62 (= G48), G64 (= G50), K65 (= K51), D187 (= D171), E188 (= E172)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
38% identity, 67% coverage: 21:271/377 of query aligns to 34:289/382 of 7aheC

query
sites
7aheC

K

K

R

K

S

T

T

G

G

A

K

T

A

V

F

G

T

V

L

Y

D

D

V

T

S

N

F

F

E

E

M

I

-

N

D

E

N

G

E

E

L

I

V

F

V

V

L

I

F

M

G

G

P

L

S

S

G

G

S

S

G

G

K

K

T

S

T

T

L

L

F

L

K

R

C

L

I

L

S

N

G

R

I

L

T

I

Q

E

P

P

D

T

N

S

G

G

K

K

I

I

T

F

V

I

G

D

S

N

K

Q

I

D

Y

V

Y

A

D

T

K

L

D

N

K

K

K

E

I

D

N

L

L

L

P

Q

I

V

Q

R

K

R

R

K

N

T

L

M

G

S

Y

M

V

V

F

F

Q

Q

N

N

Y

F

T

G

L

L

F

F

P

P

H

H

M

R

N

T

V

I

R

L

K

E

N

N

I

T

E

E

C

Y

G

G

L

L

-

E

-

V

K

Q

K

N

W

V

E

P

K

K

E

E

D

E

R

R

E

R

V

K

R

R

V

A

M

E

E

K

M

A

L

L

N

D

L

N

L

A

H

N

I

L

E

L

E

D

L

F

E

K

T

D

R

Q

Y

Y

P

P

S

K

Q

Q

I

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

P

N

K

D

P

P

G

E

I

I

L

L

L

M

D

D

E

E

P

A

F

F

S

S

A

A

L

L

D

D

M

P

E

L

I

I

R

R

T

R

E

E

L

M

A

Q

D

D

K

E

I

L

K

L

N

E

L

L

Q

Q

K

A

K

K

I

F

E

Q

I

K

P

T

L

I

F

F

I

V

T

S

H

H

N

D

L

L

E

N

E

E

A

A

F

L

L

R

L

I

A

G

D

D

R

R

I

I

L

A

I

I

L

M

H

K

G

D

G

G

K

K

I

I

Q

M

Q

Q

F

I

G

G

T

T

P

G

E

E

I

I

F

L

Y

T

Q

N

P

P

A

A

N

N

L

D

Q

Y

V

V

S

K

E

T

L

F

I

V

-

E

-

D

G

T

I

A

S

E

N

N

I

I

F

M

D

I

D

P

A

A

Y
x
L

V

T

E

T

E

N

Y

I

D

D

K

V

E

D

S

G

K

P

S

S

T

V

V

A

L

L

R

K

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ Y257)

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 297, 298

8hplC Lpqy-sugabc in state 1 (see paper)
37% identity, 60% coverage: 22:248/377 of query aligns to 14:233/384 of 8hplC

query
sites
8hplC

R

R

S

A

T

A

G

V

K

K

A

E

F

F

T

S

L

M

D

T

V

I

S

A

F

-

E

-

M

-

D

D

N

G

E

E

L

F

V

I

L

L

F

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

F

L

K

N

C

M

I

I

S

A

G

G

I

L

T

E

Q

E

P

I

D

T

N

S

G

G

K

E

I

L

T

R

V

I

G

G

S

G

K

E

I

R

Y

V

Y

N

D

E

K

K

D

A

K

P

K

K

I

-

N

-

L

-

P

-

I

-

Q

-

K

D

R

R

N

D

L

I

G

A

Y

M

V

V

F

F

Q

Q

N

S

Y

Y

T

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

R

R

K

Q

N

N

I

I

E

A

-

F

-

P

C

L

G

T

L

L

K

A

K

K

W

V

E

P

K

K

E

A

D

E

R

I

E

A

V

A

R

K

V

V

M

E

E

E

M

T

L

A

N

K

L

I

L

L

H

D

I

L

E

S

E

E

L

L

E

L

T

D

R

R

Y

K

P

P

S

G

Q

Q

I

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

K

S

P

P

G

K

I

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

K

I

L

R

R

T

V

E

Q

L

M

A

R

D

A

K

E

I

I

K

S

N

R

L

L

Q

Q

K

D

K

R

I

L

E

G

I

T

P

T

L

T

L

V

F

Y

I

V

T

T

H

H

N

D

L

Q

E

T

E

E

A

A

F

M

L

T

L

L

A

G

D

D

R

R

I

V

L

V

I

V

L

M

H

L

G

A

G

G

K

E

I

V

Q

Q

F

I

G

G

T

T

P

P

E

D

E

E

I

L

F

Y

Y

S

Q

S

P

P

A

A

N

N

L

L

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

binding adenosine-5'-triphosphate: G37 (= G48), C38 (≠ S49), G39 (= G50), K40 (= K51), S41 (≠ T52), T42 (= T53)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
37% identity, 60% coverage: 22:248/377 of query aligns to 16:235/362 of 8hprD

query
sites
8hprD

R

R

S

A

T

A

G

V

K

K

A

E

F

F

T

S

L

M

D

T

V

I

S

A

F

-

E

-

M

-

D

D

N

G

E

E

L

F

V

I

L

L

F

V

G

G

P

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

F

L

K

N

C

M

I

I

S

A

G

G

I

L

T

E

Q

E

P

I

D

T

N

S

G

G

K

E

I

L

T

R

V

I

G

G

S

G

K

E

I

R

Y

V

Y

N

D

E

K

K

D

A

K

P

K

K

I

-

N

-

L

-

P

-

I

-

Q

-

K

D

R

R

N

D

L

I

G

A

Y

M

V

V

F

F

Q
|
Q

N

S

Y

Y

T

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

R

R

K

Q

N

N

I

I

E

A

-

F

-

P

C

L

G

T

L

L

K

A

K

K

W

V

E

P

K

K

E

A

D

E

R

I

E

A

V

A

R

K

V

V

M

E

E

E

M

T

L

A

N

K

L

I

L

L

H

D

I

L

E

S

E

E

L

L

E

L

T

D

R
|
R

Y

K

P

P

S

G

Q
|
Q

I

L

S
|
S

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

K

S

P

P

G

K

I

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

K

I

L

R

R

T

V

E

Q

L

M

A

R

D

A

K

E

I

I

K

S

N

R

L

L

Q

Q

K

D

K

R

I

L

E

G

I

T

P

T

L

T

L

V

F

Y

I

V

T

T

H
|
H

N

D

L

Q

E

T

E

E

A

A

F

M

L

T

L

L

A

G

D

D

R

R

I

V

L

V

I

V

L

M

H

L

G

A

G

G

K

E

I

V

Q

Q

F

I

G

G

T

T

P

P

E

D

E

E

I

L

F

Y

Y

S

Q

S

P

P

A

A

N

N

L

L

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

binding adenosine-5'-triphosphate: S38 (= S47), C40 (≠ S49), G41 (= G50), K42 (= K51), S43 (≠ T52), T44 (= T53), Q82 (= Q97), R129 (= R142), Q133 (= Q146), S135 (= S148), G136 (= G149), G137 (= G150), Q159 (≠ E172), H192 (= H205)
binding magnesium ion: S43 (≠ T52), Q82 (= Q97)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
37% identity, 60% coverage: 22:248/377 of query aligns to 16:235/363 of 8hprC

query
sites
8hprC

R

R

S

A

T

A

G

V

K

K

A

E

F

F

T

S

L

M

D

T

V

I

S

A

F

-

E

-

M

-

D

D

N

G

E

E

L

F

V

I

L

L

F

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T

T

L

T

F

L

K

N

C

M

I

I

S

A

G

G

I

L

T

E

Q

E

P

I

D

T

N

S

G

G

K

E

I

L

T

R

V

I

G

G

S

G

K

E

I

R

Y

V

Y

N

D

E

K

K

D

A

K

P

K

K

I

-

N

-

L

-

P

-

I

-

Q

-

K

D

R

R

N

D

L

I

G

A

Y

M

V

V

F

F

Q
|
Q

N

S

Y

Y

T

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

R

R

K

Q

N

N

I

I

E

A

-

F

-

P

C

L

G

T

L

L

K

A

K

K

W

V

E

P

K

K

E

A

D

E

R

I

E

A

V

A

R

K

V

V

M

E

E

E

M

T

L

A

N

K

L

I

L

L

H

D

I

L

E

S

E

E

L

L

E

L

T

D

R

R

Y

K

P

P

S

G

Q
|
Q

I

L

S

S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

K

S

P

P

G

K

I

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

K

I

L

R

R

T

V

E

Q

L

M

A

R

D

A

K

E

I

I

K

S

N

R

L

L

Q

Q

K

D

K

R

I

L

E

G

I

T

P

T

L

T

L

V

F

Y

I

V

T

T

H
|
H

N

D

L

Q

E

T

E

E

A

A

F

M

L

T

L

L

A

G

D

D

R

R

I

V

L

V

I

V

L

M

H

L

G

A

G

G

K

E

I

V

Q

Q

F

I

G

G

T

T

P

P

E

D

E

E

I

L

F

Y

Y

S

Q

S

P

P

A

A

N

N

L

L

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

binding adenosine-5'-triphosphate: S38 (= S47), G39 (= G48), G41 (= G50), K42 (= K51), S43 (≠ T52), Q82 (= Q97), Q133 (= Q146), G136 (= G149), G137 (= G150), Q138 (= Q151), H192 (= H205)
binding magnesium ion: S43 (≠ T52), Q82 (= Q97)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

7ahdC Opua (e190q) occluded (see paper)
40% identity, 58% coverage: 21:240/377 of query aligns to 34:256/260 of 7ahdC

query
sites
7ahdC

K

K

R

K

S

T

T

G

G

A

K
x
T

A

V

F

G

T

V

L

Y

D

D

V

T

S

N

F

F

E

E

M

I

-

N

D

E

N

G

E

E

L

I

V

F

V

V

L

I

F

M

G

G

P

L

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

F

L

K

R

C

L

I

L

S

N

G

R

I

L

T

I

Q

E

P

P

D

T

N

S

G

G

K

K

I

I

T

F

V

I

G

D

S

N

K

Q

I

D

Y

V

Y

A

D

T

K

L

D

N

K

K

K

E

I

D

N

L

L

L

P

Q

I

V

Q

R

K

R

R

K

N

T

L

M

G

S

Y

M

V

V

F

F

Q
|
Q

N

N

Y

F

T

G

L

L

F

F

P

P

H

H

M

R

N

T

V

I

R

L

K

E

N

N

I

T

E

E

C

Y

G

G

L

L

-

E

-

V

K

Q

K

N

W

V

E

P

K

K

E

E

D

E

R

R

E

R

V

K

R

R

V

A

M

E

E

K

M

A

L

L

N

D

L

N

L

A

H

N

I

L

E

L

E

D

L

F

E

K

T

D

R

Q

Y

Y

P

P

S
x
K

Q
|
Q

I

L

S
|
S

G

G

G
|
G

Q
x
M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

P

N

K

D

P

P

G

E

I

I

L

L

L

M

D

D

E
x
Q

P

A

F

F

S

S

A

A

L

L

D

D

M

P

E

L

I

I

R

R

T

R

E

E

L

M

A

Q

D

D

K

E

I

L

K

L

N

E

L

L

Q

Q

K

A

K

K

I

F

E

Q

I

K

P

T

L

I

F

F

I

V

T

S

H
|
H

N

D

L

L

E

N

E

E

A

A

F

L

L

R

L

I

A

G

D

D

R

R

I

I

L

A

I

I

L

M

H

K

G

D

G

G

K

K

I

I

Q

M

Q

Q

F

I

G

G

T

T

P

G

E

E

I

I

F

L

Y

T

Q

N

P

P

A

A

N

N

binding adenosine-5'-triphosphate: T39 (≠ K26), S61 (= S47), G62 (= G48), G64 (= G50), K65 (= K51), S66 (≠ T52), T67 (= T53), Q111 (= Q97), K161 (≠ S145), Q162 (= Q146), S164 (= S148), G166 (= G150), M167 (≠ Q151), Q188 (≠ E172), H221 (= H205)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
35% identity, 73% coverage: 25:301/377 of query aligns to 16:283/353 of 1oxvD

query
sites
1oxvD

G

G

K

K

A

V

F

V

T

A

L

L

D

D

-

N

V

V

S

N

F

I

E

N

M

I

D

E

N

N

-

G

E

E

L

R

V

F

V

G

L

I

F

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

F

M

K

R

C

I

I

I

S

A

G

G

I

L

T

D

Q

V

P

P

D

S

N

T

G

G

K

E

I

L

T

Y

V

F

G

D

S

D

K

R

I

L

Y

V

Y

A

D

S

K

N

D

G

K

K

K

L

I

I

N

-

L

V

P

P

I

P

Q

E

K

D

R

R

N

K

L

I

G

G

Y

M

V

V

F

F

Q
|
Q

N

T

Y

W

T

A

L

L

F

Y

P

P

H

N

M

L

N

T

V

A

R

F

K

E

N

N

I

I

E

A

C

F

G

P

L

L

K

T

-

N

-

M

K

K

W

M

E

S

K

K

E

E

D

E

R

I

E

R

V

K

R

R

V

V

M

E

E

E

M

V

L

A

N

K

L

I

L

L

H

D

I

I

E

H

L

V

E

L

T

N

R

H

Y

F

P

P

S

R

Q

E

I

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

P

K

K

D

P

P

G

S

I

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

L

L

D

D

M

A

E

R

I

M

R

R

T

D

E

S

L

A

A

R

D

A

K

L

I

V

K

K

N

E

L

V

Q

Q

K

S

K

R

I

L

E

G

I

V

P

T

L

L

F

V

I

V

T

S

H

H

N

D

L

P

E

A

E

D

A

I

F

F

L

A

L

I

A

A

D

D

R

R

I

V

L

G

I

V

L

L

H

V

G

K

G

G

K

K

I

L

Q

V

Q

Q

F

V

G

G

T

K

P

P

E

E

E

D

I

L

F

Y

Y

D

Q

N

P

P

A

V

N

S

L

I

Q

Q

V

V

S

A

E

S

L

L

I

I

G

G

I

E

S

I

N

N

I

-

F

-

D

-

A

-

Y

-

V

-

E

-

Y

-

D

-

K

-

E

E

S

L

K

E

S

G

T

K

V

V

L

T

R

N

S

E

G

G

D

V

M

V

R

I

I

G

K

S

I

L

E

R

S

F

P

P

N

V

F

S

K

V

A

S

G

S

D

D

K

R

V

A

T

I

W

I

G

G

I

I

Y

R

P

P

E

E

N

D

I

V

T

K

L

L

binding phosphoaminophosphonic acid-adenylate ester: S40 (= S47), G41 (= G48), G43 (= G50), K44 (= K51), T45 (= T52), T46 (= T53), Q89 (= Q97), E166 (= E172)
binding magnesium ion: T45 (= T52), Q89 (= Q97)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
35% identity, 73% coverage: 25:301/377 of query aligns to 16:283/353 of 1oxvA

query
sites
1oxvA

G

G

K

K

A

V

F

V

T

A

L

L

D

D

-

N

V

V

S

N

F

I

E

N

M

I

D

E

N

N

-

G

E

E

L

R

V

F

V

G

L

I

F

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

F

M

K

R

C

I

I

I

S

A

G

G

I

L

T

D

Q

V

P

P

D

S

N

T

G

G

K

E

I

L

T

Y

V

F

G

D

S

D

K

R

I

L

Y

V

Y

A

D

S

K

N

D

G

K

K

K

L

I

I

N

-

L

V

P

P

I

P

Q

E

K

D

R

R

N

K

L

I

G

G

Y

M

V

V

F

F

Q

Q

N

T

Y

W

T

A

L

L

F

Y

P

P

H

N

M

L

N

T

V

A

R

F

K

E

N

N

I

I

E

A

C

F

G

P

L

L

K

T

-

N

-

M

K

K

W

M

E

S

K

K

E

E

D

E

R

I

E

R

V

K

R

R

V

V

M

E

E

E

M

V

L

A

N

K

L

I

L

L

H

D

I

I

E

H

L

V

E

L

T

N

R

H

Y

F

P

P

S

R

Q

E

I

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

P

K

K

D

P

P

G

S

I

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

L

L

D

D

M

A

E

R

I

M

R

R

T

D

E

S

L

A

A

R

D

A

K

L

I

V

K

K

N

E

L

V

Q

Q

K

S

K

R

I

L

E

G

I

V

P

T

L

L

F

V

I

V

T

S

H

H

N

D

L

P

E

A

E

D

A

I

F

F

L

A

L

I

A

A

D

D

R

R

I

V

L

G

I

V

L

L

H

V

G

K

G

G

K

K

I

L

Q

V

Q

Q

F

V

G

G

T

K

P

P

E

E

E

D

I

L

F

Y

Y

D

Q

N

P

P

A

V

N

S

L

I

Q

Q

V

V

S

A

E

S

L

L

I

I

G

G

I

E

S

I

N

N

I

-

F

-

D

-

A

-

Y

-

V

-

E

-

Y

-

D

-

K

-

E

E

S

L

K

E

S

G

T

K

V

V

L

T

R

N

S

E

G

G

D

V

M

V

R

I

I

G

K

S

I

L

E

R

S

F

P

P

N

V

F

S

K

V

A

S

G

S

D

D

K

R

V

A

T

I

W

I

G

G

I

I

Y

R

P

P

E

E

N

D

I

V

T

K

L

L

binding phosphoaminophosphonic acid-adenylate ester: S40 (= S47), G41 (= G48), G43 (= G50), K44 (= K51), T45 (= T52), T46 (= T53), E166 (= E172)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
35% identity, 73% coverage: 25:301/377 of query aligns to 16:283/353 of 1oxuA

query
sites
1oxuA

G

G

K

K

A

V

F

V

T
x
A

L

L

D

D

-

N

V

V

S

N

F

I

E

N

M

I

D

E

N

N

-

G

E

E

L

R

V

F

V

G

L

I

F

L

G

G

P

P

S

S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

F

M

K

R

C

I

I

I

S

A

G

G

I

L

T

D

Q

V

P

P

D

S

N

T

G

G

K

E

I

L

T

Y

V

F

G

D

S

D

K

R

I

L

Y

V

Y

A

D

S

K

N

D

G

K

K

K

L

I

I

N

-

L

V

P

P

I

P

Q

E

K

D

R

R

N

K

L

I

G

G

Y

M

V

V

F

F

Q

Q

N

T

Y

W

T

A

L

L

F

Y

P

P

H

N

M

L

N

T

V

A

R

F

K

E

N

N

I

I

E

A

C

F

G

P

L

L

K

T

-

N

-

M

K

K

W

M

E

S

K

K

E

E

D

E

R

I

E

R

V

K

R

R

V

V

M

E

E

E

M

V

L

A

N

K

L

I

L

L

H

D

I

I

E

H

L

V

E

L

T

N

R

H

Y

F

P

P

S

R

Q

E

I

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

P

K

K

D

P

P

G

S

I

L

L

L

D

D

E

E

P

P

F

F

S

S

A

N

L

L

D

D

M

A

E

R

I

M

R

R

T

D

E

S

L

A

A

R

D

A

K

L

I

V

K

K

N

E

L

V

Q

Q

K

S

K

R

I

L

E

G

I

V

P

T

L

L

F

V

I

V

T

S

H

H

N

D

L

P

E

A

E

D

A

I

F

F

L

A

L

I

A

A

D

D

R

R

I

V

L

G

I

V

L

L

H

V

G

K

G

G

K

K

I

L

Q

V

Q

Q

F

V

G

G

T

K

P

P

E

E

E

D

I

L

F

Y

Y

D

Q

N

P

P

A

V

N

S

L

I

Q

Q

V

V

S

A

E

S

L

L

I

I

G

G

I

E

S

I

N

N

I

-

F

-

D

-

A

-

Y

-

V

-

E

-

Y

-

D

-

K

-

E

E

S

L

K

E

S

G

T

K

V

V

L

T

R

N

S

E

G

G

D

V

M

V

R

I

I

G

K

S

I

L

E

R

S

F

P

P

N

V

F

S

K

V

A

S

G

S

D

D

K

R

V

A

T

I

W

I

G

G

I

I

Y

R

P

P

E

E

N

D

I

V

T

K

L

L

binding adenosine-5'-diphosphate: A20 (≠ T29), G41 (= G48), G43 (= G50), K44 (= K51), T45 (= T52), T46 (= T53)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
35% identity, 73% coverage: 25:301/377 of query aligns to 16:283/353 of Q97UY8

query
sites
Q97UY8

G

G

K

K

A

V

F

V

T

A

L

L

D

D

-

N

V

V

S

N

F

I

E

N

M

I

D

E

N

N

-

G

E

E

L

R

V

F

V

G

L

I

F

L

G

G

P

P

S

S

G

G

S

A

G

G

K

K

T

T

L

F

F

M

K

R

C

I

I

I

S

A

G

G

I

L

T

D

Q

V

P

P

D

S

N

T

G

G

K

E

I

L

T

Y

V

F

G

D

S

D

K

R

I

L

Y

V

Y

A

D

S

K

N

D

G

K

K

K

L

I

I

N

-

L

V

P

P

I

P

Q

E

K

D

R

R

N

K

L

I

G

G

Y

M

V

V

F

F

Q

Q

N

T

Y

W

T

A

L

L

F

Y

P

P

H

N

M

L

N

T

V

A

R

F

K

E

N

N

I

I

E

A

C

F

G

P

L

L

K

T

-

N

-

M

K

K

W

M

E

S

K

K

E

E

D

E

R

I

E

R

V

K

R

R

V

V

M

E

E

E

M

V

L

A

N

K

L

I

L

L

H

D

I

I

E

H

L

V

E

L

T

N

R

H

Y

F

P

P

S

R

Q

E

I

L

S
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S

G

G

G
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G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

P

K

K

D

P

P

G

S

I

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

L

L

D

D

M

A

E

R

I

M

R

R

T

D

E

S

L

A

A

R

D

A

K

L

I

V

K

K

N

E

L

V

Q

Q

K

S

K

R

I

L

E

G

I

V

P

T

L

L

F

V

I

V

T

S

H

H

N

D

L

P

E

A

E

D

A

I

F

F

L

A

L

I

A

A

D

D

R

R

I

V

L

G

I

V

L

L

H

V

G

K

G

G

K

K

I

L

Q

V

Q

Q

F

V

G

G

T

K

P

P

E

E

E

D

I

L

F

Y

Y

D

Q

N

P

P

A

V

N

S

L

I

Q

Q

V

V

S

A

E

S

L

L

I

I

G

G

I

E

S

I

N

N

I

-

F

-

D

-

A

-

Y

-

V

-

E

-

Y

-

D

-

K

-

E

E

S

L

K

E

S

G

T

K

V

V

L

T

R

N

S

E

G

G

D

V

M

V

R

I

I

G

K

S

I

L

E

R

S

F

P

P

N

V

F

S

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V

A

S

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S

D

D

K

R

V

A

T

I

W

I

G

G

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I

Y

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P

E

E

N

D

I

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L

L

S142 (= S148) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G150) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E172) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 74% coverage: 26:303/377 of query aligns to 20:290/369 of P19566

query
sites
P19566

K

K

A

D

F

I

T

N

L

L

D

D

V

I

S

H

F

-

E

-

M

-

D

D

N

G

E

E

L

F

V

V

L

F

F

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

F

L

K

R

C

M

I

I

S

A

G

G

I

L

T

E

Q

T

P

I

D

T

N

S

G

G

K

D

I

L

T

F

V

I

G

G

S

E

K

T

I

R

Y

M

Y

N

D

D

K

-

D

-

K

-

I

-

N

-

L

I

P

P

I

P

Q

A

K

E

R

R

N

G

L

V

G

G

Y

M

V

V

F

F

Q

Q

N

S

Y

Y

T

A

L
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L

F

Y

P

P

H

H

M

L

N

S

V

V

R

A

K

E

N

N

I

M

E

S

C

F

G

G

L

L

K

K

-

L

-

A

K

G

W

A

E

K

K

K

E

E

D

V

R

M

E

N

V

Q

R

R

V

V

M

N

E

Q

M

V

L

A

N

E

L

V

L

L

H

Q

I

L

E

A

E

H

L

L

E

L

T

E

R

R

Y

K

P

P

S

K

Q

A

I

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

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V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

K

E

P

P

G

R

I

V

L

F

L

L

D

D

E

E

P
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P

F

L

S

S

A

N

L

L

D
|
D

M

A

E

A

I

L

R

R

T

V

E

Q

L

M

A

R

D

I

K

E

I

I

K

S

N

R

L

L

Q

H

K

K

K

R

I

L

E

G

I

R

P

T

L

M

L

I

F

Y

I

V

T

T

H

H

N

D

L

Q

E

V

E

E

A

A

F

M

L

T

L

L

A

A

D

D

R

K

I

I

L

V

I

V

L

L

H

D

G

A

G

G

K

R

I

V

Q

A

Q

Q

F

V

G

G

T

K

P

P

E

L

E

E

I

L

F

Y

Y

H

Q

Y

P

P

A

A

N

D

L

R

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

I

S

S

P

-

K

-

M

N

N

I

F

F

L

D

P

D

V

A

K

Y

V

V

T

E

A

E

T

Y

A

D

I

K

E

E

Q

S

V

K

Q

S

V

T

E

V

L

L

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N

S

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G

Q

D

Q

M

I

R

W

I

L

K

P

I

V

E

E

S

S

P

R

N

G

F

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K

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A

V

G

G

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A

K

N

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M

T

S

W

L

G

G

I

I

Y

R

P

P

E

E

N

H

I

-

T

-

L

L

P

P

L86 (= L101) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 84% coverage: 26:343/377 of query aligns to 20:328/371 of P68187

query
sites
P68187

K

K

A

D

F

I

T

N

L

L

D

D

V

I

S

H

F

-

E

-

M

-

D

E

N

G

E

E

L

F

V

V

L

F

F

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

F

L

K

R

C

M

I

I

S

A

G

G

I

L

T

E

Q

T

P

I

D

T

N

S

G

G

K

D

I

L

T

F

V

I

G

G

S

E

K

K

I

R

Y

M

Y

N

D

D

K

T

D

-

K

-

I

-

N

-

L

-

P

P

I

P

Q

A

K

E

R

R

N

G

L

V

G

G

Y

M

V

V

F

F

Q

Q

N

S

Y

Y

T
x
A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

R

A

K

E

N

N

I

M

E

S

C

F

G

G

L

L

K

K

-

L

-

A

K

G

W

A

E
x
K

K

K

E

E

D

V

R

I

E

N

V

Q

R

R

V
|
V

M

N

E

Q

M
x
V

L

A

N
x
E

L

V

L

L

H

Q

I

L

E
x
A

E

H

L

L

E

L

T

D

R

R

Y

K

P

P

S

K

Q

A

I

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

K

E

P

P

G

S

I

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

M

A

E

A

I

L

R

R

T

V

E

Q

L

M

A

R

D

I

K

E

I

I

K

S

N

R

L

L

Q

H

K

K

K

R

I

L

E

G

I

R

P

T

L

M

L

I

F

Y

I

V

T

T

H

H

N

D

L

Q

E

V

E

E

A

A

F

M

L

T

L

L

A

A

D

D

R

K

I

I

L

V

I

V

L

L

H

D

G

A

G

G

K

R

I

V

Q

A

Q

Q

F

V

G

G

T

K

P

P

E

L

E

E

I

L

F

Y

Y

H

Q

Y

P

P

A

A

N

D

L
x
R

Q

F

V

V

S

A

E

G

L

F

I

I

G

G

-

S

-

P

-

K

-

M

-

N

-
x
F

-

L

-

P

-

V

-

K

I

V

S

T

N

A

I

T

F

A

D

I

D

D

A

Q

Y

V

V

Q

E

V

E

E

Y

L

D

P

K

M

E

P

S

N

K

R

S

Q

T

Q

V

V

L
x
W

R

-

S

-

G

-

D

-

M

-

R

-

I

L

K

P

I

V

E

E

S

S

P

R

N

D

F

V

K

Q

A

V

G
|
G

D

A

K

N

V

M

T
x
S

W

L

G
|
G

I

I

Y

R

P

P

E

E

N

H

I

-

T

-

L

L

P

P

V

-

S

-

G

-

S

-

E

S

D

D

Q

I

D

A

E

D

N

V

I

I

Y

L

S

E

A
x
G

H

E

V

V

N

Q

N

V

I

V

I
x
E

N

Q

K

L

G

G

P

N

K

E

K

T

R

Q

I

I

T

H

L

I

K

Q

L

I

V

P

R
x
S

Y

I

N

R

K

Q

T

N

L

L

I

V

A85 (≠ T100) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ E119) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V127) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M130) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ N132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ L241) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (vs. gap) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (≠ L270) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (= G287) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ T291) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (= G293) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (≠ A317) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ I323) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
S322 (≠ R337) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

Query Sequence

>WP_011021256.1 NCBI__GCF_000007345.1:WP_011021256.1
MRLGVKVDIKKHYTEAEINRKRSTGKAFTLDVSFEMDNELVVLFGPSGSGKTTLFKCISG
ITQPDNGKITVGSKIYYDKDKKINLPIQKRNLGYVFQNYTLFPHMNVRKNIECGLKKWEK
EDREVRVMEMLNLLHIEELETRYPSQISGGQKQRVALARALAPKPGILLLDEPFSALDME
IRTELADKIKNLQKKIEIPLLFITHNLEEAFLLADRILILHGGKIQQFGTPEEIFYQPAN
LQVSELIGISNIFDDAYVEEYDKESKSTVLRSGDMRIKIESPNFKAGDKVTWGIYPENIT
LLPVSGSEDQDENIYSAHVNNIINKGPKKRITLKLVRYNKTLIAEVPAQFVDSLELHAGG
FCLVRLEMNKVVAFRNF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory