SitesBLAST
Comparing WP_011021352.1 NCBI__GCF_000007345.1:WP_011021352.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
45% identity, 88% coverage: 27:306/319 of query aligns to 25:304/304 of 1wwkA
- active site: S96 (≠ A98), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H280)
- binding nicotinamide-adenine-dinucleotide: V100 (= V102), G146 (= G148), F147 (≠ T149), G148 (= G150), R149 (≠ E151), I150 (= I152), Y168 (≠ V170), D169 (≠ T171), P170 (≠ A172), V201 (= V203), P202 (= P204), T207 (= T209), T228 (= T230), S229 (≠ A231), D254 (= D256), H278 (= H280), G280 (≠ A282)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
40% identity, 90% coverage: 33:318/319 of query aligns to 34:327/334 of 5aovA
- active site: L100 (≠ A98), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H280)
- binding glyoxylic acid: M52 (≠ V50), L53 (≠ G51), L53 (≠ G51), Y74 (≠ W72), A75 (≠ Q73), V76 (≠ T74), G77 (= G75), R241 (= R232), H288 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T74), T104 (≠ V102), F158 (≠ T149), G159 (= G150), R160 (≠ E151), I161 (= I152), S180 (≠ T171), R181 (≠ A172), A211 (≠ H202), V212 (= V203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), H288 (= H280), G290 (≠ A282)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
42% identity, 77% coverage: 67:312/319 of query aligns to 77:334/336 of 5z20F
- active site: S108 (≠ A98), R241 (= R232), D265 (= D256), E270 (= E261), H302 (= H280)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y97), G160 (= G150), Q161 (≠ E151), I162 (= I152), Y180 (≠ V170), D181 (≠ T171), P182 (≠ A172), C212 (≠ V203), P213 (= P204), T218 (= T209), T239 (= T230), G240 (≠ A231), R241 (= R232), H302 (= H280), A304 (= A282)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
41% identity, 88% coverage: 38:318/319 of query aligns to 38:326/332 of 6biiA
- active site: L99 (≠ A98), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ T74), T103 (≠ V102), G156 (= G148), F157 (≠ T149), G158 (= G150), R159 (≠ E151), I160 (= I152), A179 (≠ T171), R180 (≠ A172), S181 (≠ H173), K183 (≠ S175), V211 (= V203), P212 (= P204), E216 (= E208), T217 (= T209), V238 (≠ T230), A239 (= A231), R240 (= R232), D264 (= D256), H287 (= H280), G289 (≠ A282)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
39% identity, 85% coverage: 36:307/319 of query aligns to 34:305/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
39% identity, 85% coverage: 36:307/319 of query aligns to 33:304/526 of 3dc2A
Sites not aligning to the query:
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
37% identity, 87% coverage: 40:315/319 of query aligns to 38:334/334 of 3kb6B
- active site: S97 (≠ A98), R231 (= R232), D255 (= D256), E260 (= E261), H294 (= H280)
- binding lactic acid: F49 (≠ G51), S72 (≠ Q73), V73 (≠ T74), G74 (= G75), Y96 (= Y97), R231 (= R232), H294 (= H280)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ T74), Y96 (= Y97), V101 (= V102), G150 (= G150), R151 (≠ E151), I152 (= I152), D171 (≠ T171), V172 (≠ A172), P203 (= P204), T229 (= T230), A230 (= A231), R231 (= R232), H294 (= H280), A296 (= A282), Y297 (≠ F283)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
38% identity, 76% coverage: 66:307/319 of query aligns to 69:322/330 of 4cukA
- active site: S101 (≠ A98), R234 (= R232), D258 (= D256), E263 (vs. gap), H295 (= H280)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y97), G153 (= G150), K154 (≠ E151), I155 (= I152), F173 (≠ V170), D174 (≠ T171), P175 (≠ A172), H204 (= H202), C205 (≠ V203), P206 (= P204), N211 (≠ T209), T232 (= T230), Y260 (≠ F258), H295 (= H280), A297 (= A282)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
31% identity, 99% coverage: 2:317/319 of query aligns to 8:321/533 of O43175
- T78 (= T74) binding NAD(+)
- R135 (≠ W131) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ EI 151:152) binding NAD(+)
- D175 (≠ T171) binding NAD(+)
- T207 (≠ V203) binding NAD(+)
- CAR 234:236 (≠ TAR 230:232) binding NAD(+)
- D260 (= D256) binding NAD(+)
- V261 (= V257) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAF 280:283) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 3:301/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (= L147), G147 (= G148), L148 (≠ T149), G149 (= G150), R150 (≠ E151), I151 (= I152), G152 (= G153), D170 (≠ T171), H201 (= H202), T202 (≠ V203), P203 (= P204)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 3:301/302 of 6rihA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 4:302/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 4:302/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (= T74), A102 (≠ V102), G148 (= G148), R151 (≠ E151), I152 (= I152), Y170 (≠ V170), D171 (≠ T171), P172 (≠ A172), I173 (≠ H173), H202 (= H202), T203 (≠ V203), P204 (= P204), T209 (= T209), C230 (≠ T230), A231 (= A231), R232 (= R232), H279 (= H280), G281 (≠ A282)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ Y15), K17 (≠ R18), I18 (≠ L19), E293 (vs. gap)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 3:301/301 of 6rj5A
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
31% identity, 94% coverage: 2:302/319 of query aligns to 3:301/303 of 6plgA