SitesBLAST
Comparing WP_011021688.1 NCBI__GCF_000007345.1:WP_011021688.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3nemB Aspartyl-tRNA synthetase complexed with aspartyl adenylate (see paper)
54% identity, 99% coverage: 7:444/444 of query aligns to 3:438/438 of 3nemB
- active site: R214 (= R220), E216 (= E222), R222 (= R228), H223 (= H229), E361 (= E367), S364 (= S370), R412 (= R418)
- binding adenosine-5'-triphosphate: R214 (= R220), E216 (= E222), H223 (= H229), L224 (= L230), E361 (= E367), I362 (≠ L368), S363 (= S369), S364 (= S370), G407 (= G413), G409 (= G415), R412 (= R418)
- binding magnesium ion: E361 (= E367), S364 (= S370)
3nemA Aspartyl-tRNA synthetase complexed with aspartyl adenylate (see paper)
54% identity, 99% coverage: 7:444/444 of query aligns to 3:438/438 of 3nemA
- active site: R214 (= R220), E216 (= E222), R222 (= R228), H223 (= H229), E361 (= E367), S364 (= S370), R412 (= R418)
- binding aspartyl-adenosine-5'-monophosphate: E170 (= E176), Q192 (= Q198), K195 (= K201), R214 (= R220), E216 (= E222), H223 (= H229), L224 (= L230), Y339 (= Y345), E361 (= E367), I362 (≠ L368), S363 (= S369), S364 (= S370), G365 (= G371), R368 (= R374), F406 (≠ W412), G407 (= G413), G409 (= G415), R412 (= R418)
3nelA Aspartyl-tRNA synthetase complexed with aspartic acid (see paper)
54% identity, 99% coverage: 7:444/444 of query aligns to 3:438/438 of 3nelA
- active site: R214 (= R220), E216 (= E222), R222 (= R228), H223 (= H229), E361 (= E367), S364 (= S370), R412 (= R418)
- binding aspartic acid: E170 (= E176), Q192 (= Q198), K195 (= K201), Y339 (= Y345), S364 (= S370), R368 (= R374), F406 (≠ W412), G407 (= G413)
Q52428 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
54% identity, 99% coverage: 7:444/444 of query aligns to 3:438/438 of Q52428
- W26 (≠ H32) mutation to H: Gains the ability to form Asp-tRNA(Asn) in vitro. Only 2-fold decrease in catalytic efficiency for Asp-tRNA(Asp) synthesis.
- K85 (≠ P91) mutation to P: Gains the ability to form Asp-tRNA(Asn) in vitro, and is impaired in its ability to synthesize Asp-tRNA(Asp) due to a 8-fold decrease in affinity for tRNA(Asp).
1b8aA Aspartyl-tRNA synthetase (see paper)
54% identity, 99% coverage: 7:444/444 of query aligns to 3:438/438 of 1b8aA
- binding adenosine-5'-triphosphate: R214 (= R220), E216 (= E222), H223 (= H229), L224 (= L230), E361 (= E367), I362 (≠ L368), S363 (= S369), S364 (= S370), G409 (= G415), R412 (= R418)
- binding manganese (ii) ion: E361 (= E367), S364 (= S370)
O07683 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) (see paper)
41% identity, 99% coverage: 6:444/444 of query aligns to 2:436/436 of O07683
- H26 (= H32) mutation H->A,Q: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by decreasing the ability to form Asp-tRNA(Asn).
- P84 (= P91) mutation P->A,K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by decreasing the ability to form Asp-tRNA(Asn).
Q9RVH4 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase 2; AspRS2; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see paper)
38% identity, 95% coverage: 22:444/444 of query aligns to 18:435/435 of Q9RVH4
- H28 (= H32) mutation to Q: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn).
- P77 (= P91) mutation P->C,I,L,F,S,V: Seems not to be able to charge tRNA(Asn) in vivo, but Asp-tRNA(Asp) formation is not affected.; mutation to K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by decreasing the ability to form Asp-tRNA(Asn) and increasing the efficiency of Asp-tRNA(Asp) synthesis in vitro. Seems not to be able to charge tRNA(Asn) in vivo.
P04802 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
36% identity, 96% coverage: 17:444/444 of query aligns to 101:557/557 of P04802
- P273 (= P168) mutation to G: Loss of activity; important for dimerization.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
1aszA The active site of yeast aspartyl-tRNA synthetase: structural and functional aspects of the aminoacylation reaction (see paper)
36% identity, 96% coverage: 17:444/444 of query aligns to 34:490/490 of 1aszA
- active site: R258 (= R220), E260 (= E222), R266 (= R228), H267 (= H229), E411 (= E367), S414 (= S370), R464 (= R418)
- binding adenosine-5'-triphosphate: R258 (= R220), M268 (≠ L230), F271 (≠ A233), E411 (= E367), I412 (≠ L368), L413 (≠ S369), G459 (= G413), R464 (= R418)
- binding : R52 (= R35), Q53 (≠ D36), Q54 (≠ L37), T57 (vs. gap), L58 (≠ I40), F60 (= F42), Q71 (= Q53), L73 (≠ T55), E110 (≠ K89), I112 (≠ P91), K113 (≠ N92), E135 (vs. gap), P138 (= P112), L140 (vs. gap), A154 (vs. gap), L156 (vs. gap), P157 (vs. gap), V158 (vs. gap), N160 (vs. gap), T163 (= T125), S213 (≠ T175), E214 (= E176), G215 (= G177), G216 (= G178), S217 (≠ T179), Q233 (= Q195), F237 (≠ L199), E260 (= E222), N261 (≠ E223), S262 (≠ H224), N263 (≠ D225), H267 (= H229), S356 (≠ G312), T357 (= T313), F388 (= F344), K486 (≠ R440)
1asyA Class ii aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA asp (see paper)
36% identity, 96% coverage: 17:444/444 of query aligns to 34:490/490 of 1asyA
- active site: R258 (= R220), E260 (= E222), R266 (= R228), H267 (= H229), E411 (= E367), S414 (= S370), R464 (= R418)
- binding : R52 (= R35), Q53 (≠ D36), Q54 (≠ L37), L58 (≠ I40), F60 (= F42), Q71 (= Q53), L73 (≠ T55), K88 (≠ D67), P111 (≠ A90), I112 (≠ P91), K113 (≠ N92), S114 (≠ G93), E135 (vs. gap), P138 (= P112), A154 (vs. gap), L156 (vs. gap), P157 (vs. gap), V158 (vs. gap), V159 (vs. gap), D162 (= D124), T163 (= T125), R258 (= R220), E260 (= E222), N261 (≠ E223), S262 (≠ H224), N263 (≠ D225), T264 (= T226), H267 (= H229), M268 (≠ L230), F271 (≠ A233), T357 (= T313), E411 (= E367), I412 (≠ L368), L413 (≠ S369), S414 (= S370), G459 (= G413), R464 (= R418), K486 (≠ R440)
O74407 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 98% coverage: 10:444/444 of query aligns to 92:580/580 of O74407