SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011022097.1 NCBI__GCF_000007345.1:WP_011022097.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
51% identity, 93% coverage: 1:247/267 of query aligns to 1:281/285 of 4yerA

query
sites
4yerA

M

M

Q

E

N

D

I

I

L

I

S

V

V

V

K

E

S

N

L

L

T

V

K

K

F
|
F

D

G

D

D

L
x
F

T

E

A

A

V

V

K

K

G

G

I

V

S

S

F

F

D

S

V

V

E

K

T

K

G

G

S

E

I

I

F

F

A

A

F

F

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

I

I

K

H

M

M

L

L

T

T

V

L

L

L

R

K

P

P

T

T

S

S

G

G

E

K

I

A

L

W

I

V

N

A

G

G

Y

H

N

D

A

V

L

L

K

K

E

E

Q

P

D

R

K

E

A

V

R

R

S

R

S

K

F

I

G

G

I

I

V

V

F

F

Q

Q

D

D

Y

Q

S

S

L

L

D

D

S

R

E

E

L

L

T

T

A

A

Y

Y

E

E

N

N

M

M

E

Y

Y

I

H

H

S

G

V

K

I

I

Y

Y

K

G

V

Y

P

G

K

G

A

E

E

K

R

L

E

K

R

R

I

I

K

L

T

E

A

L

L

L

E

E

I

F

V

V

G

E

L

L

W

L

N

E

R

F

R

K

N

D

D

K

L

P

V

V

K

K

K
x
T

Y
x
F

S
|
S

G

G

M

M

K

A

R

R

L

L

E

E

I

I

A

A

R

R

A

S

L

L

V

I

H

H

Y

E

P

P

K

E

V

V

L

L

F

F

L

L

D

D

E

E

P

P

T

T

V

I

G

G

L

L

D

D

P

P

Q

H

T

T

R

R

M

A

S

H

I

M

W

W

N

E

H

Y

I

I

K

S

R

K

L

M

N

K

E

K

E

E

R

H

K

N

M

M

T

T

I

I

F

F

L

L

T

T

H

H

Y

Y

M

M

D

D

E

E

A

A

E

E

A

Q

M

L

A

A

D

D

K

R

I

V

A

A

I

I

D

D

H

H

G

G

K

K

I

I

E

A

S

L

G

G

T

T

L

P

A

T

E

E

I

L

M

K

E

R

R

M

T

V

E

G

T

K

E

E

-

I

-

Y

-

V

-

R

-

D

-

F

-

I

-

E

-

S

-

C

-

R

-

K

-

L

-

P

-

D

-

G

-

R

-

L

-

E

-

L

-

N

-

V

-

E

-

D

-

I

-

E

-

I

-

T

-

Y

-

H

-

K

-

P

S

T

L

L

E

N

E

D

S

V

F

F

L

L

K

H

L

L

T

T

G

G

R

R

D

E

I

L

R

R

D

E

E

E

binding adenosine-5'-diphosphate: F14 (= F14), F17 (≠ L17), N39 (= N39), G40 (= G40), G42 (= G42), K43 (= K43), T44 (≠ S44), T45 (= T45), T135 (≠ K135), F136 (≠ Y136), S137 (= S137)

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
39% identity, 79% coverage: 4:214/267 of query aligns to 989:1199/2435 of Q9BZC7

query
sites
Q9BZC7

I

V

L

V

S

C

V

V

K

D

S

K

L

L

T

T

K

K

-

V

-

Y

K

K

F

D

D

D

D

K

L

K

T

L

A

A

V

L

K

N

G

K

I

L

S

S

F

L

D

N

V

L

E

Y

T

E

G

N

S

Q

I

V

F

V

A

S

F

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

S

T

T

T

I

M

K

S

M

I

L

L

T

T

T

G

V

L

L

F

R

P

P

P

T

T

S

S

G

G

E

S

I

A

L

T

I

I

N

Y

G

G

Y

H

N

D

A

I

L

R

K

T

E

E

Q

M

D

D

K

E

A

I

R

R

S

K

S

N

F

L

G

G

I

M

V

C

F

P

Q

Q

D

H

Y

N

S

V

L

L

D

F

S

D

E

R

L

L

T

T

A

V

Y

E

E

E

N

H

M

L

E

W

Y

F

H

Y

S

S

V

R

I

L

Y

K

K

S

V

M

P

A

K

Q

A

E

E

E

R

I

E

R

R

E

I

M

K

D

T

K

A

M

L

I

E

E

I

D

V

L

G

E

L

L

W

S

N

N

R

K

R

R

N

H

D

S

L

L

V

V

K

Q

K

T

Y

L

S

S

G

G

M

M

K

K

R

R

R

K

L

L

E

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

Y

G

P

S

K

R

V

A

L

I

F

I

L

L

D

D

E

E

P

P

T

T

V

A

G

G

L

V

D

D

P

P

Q

Y

T

A

R

R

M

R

S

A

I

I

W

W

N

D

H

L

I

I

K

L

R

K

L

Y

N

K

E

P

E

G

R

R

K

-

M

-

T

T

I

I

F

L

L

L

T

S

T

T

H

H

Y

H

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

D

S

H

H

G

G

K

K

I

L

Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

8f5bA Human abca4 structure in complex with amp-pnp (see paper)
40% identity, 80% coverage: 7:219/267 of query aligns to 723:935/1924 of 8f5bA

query
sites
8f5bA

V

V

K

K

S

N

L

L

T

V

K

K

K

I

F

F

D

E

D

P

L

C

-

G

-

R

T

P

A

A

V

V

K

D

G

R

I

L

S

N

F

I

D

T

V

F

E

Y

T

E

G

N

S

Q

I

I

F

T

A

A

F

F

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

I

L

K

S

M

I

L

L

T

T

T

G

V

L

L

L

R

P

P

P

T

T

S

S

G

G

E

T

I

V

L

L

I

V

N

G

G

G

Y

R

N

D

A

I

L

E

K

T

E

S

Q

L

D

D

K

A

A

V

R

R

S

Q

S

S

F

L

G

G

I

M

V

C

F

P

Q
|
Q

D

H

Y

N

S

I

L

L

D

F

S

H

E

H

L

L

T

T

A

V

Y

A

E

E

N

H

M

M

E

L

Y

F

H

Y

S

A

V

Q

I

L

Y

K

K

G

V

K

P

S

K

Q

A

E

E

E

R

A

E

Q

E

L

R

E

I

M

K

E

T

A

A

M

L

L

E

E

I

D

V

T

G

G

L

L

W

H

N

H

R

K

R

R

N

N

D

E

L

E

V

A

K

Q

K
x
D

Y

L

S

S

G

G

M

M

K

Q

R

R

R

K

L

L

E

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

Y

D

P

A

K

K

V

V

L

V

F

I

L

L

D

D

E

E

P

P

T

T

V

S

G

G

L

V

D

D

P

P

Q

Y

T

S

R

R

M

R

S

S

I

I

W

W

N

D

H

L

I

L

K

L

R

K

L

Y

N

R

E

S

E

G

R

R

K

-

M

-

T

T

I

I

F

I

L

M

T

S

T

T

H

H

Y

H

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

D

A

H

Q

G

G

K

R

I

L

I

Y

E

C

S

S

G

G

T

T

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N39), G758 (= G40), G760 (= G42), K761 (= K43), T762 (≠ S44), T763 (= T45), Q802 (= Q84), D853 (≠ K135)
binding magnesium ion: T762 (≠ S44), Q802 (= Q84)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 89% coverage: 4:240/267 of query aligns to 1:244/343 of P30750

query
sites
P30750

I

M

L

I

S

K

V

L

K

S

S

N

L

I

T

T

K

K

K

V

F

F

D

H

D

Q

-

G

-

T

-

R

-

T

L

I

T

Q

A

A

V

L

K

N

G

N

I

V

S

S

F

L

D

H

V

V

E

P

T

A

G

G

S

Q

I

I

F

Y

A

G

F

V

L

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

I

I

K

R

M

C

L

V

T

N

T

L

V

L

L

E

R

R

P

P

T

T

S

E

G

G

E

S

I

V

L

L

I

V

N

D

G

G

-

Q

-

E

Y

L

N

T

A

T

L

L

K

S

E

E

Q

S

D

E

-

L

-

T

K

K

A

A

R

R

S

R

S

Q

F

I

G

G

I

M

V

I

F

F

Q

Q

D

H

Y

F

S

N

L

L

D

L

S

S

E

S

L

R

T

T

A

V

Y

F

E

G

N

N

M

V

E

A

Y

L

H

P

S

L

V

E

I

L

Y

D

K

N

V

T

P

P

K

K

A

D

E

E

R

V

E

K

E

R

R

R

I

V

K

T

T

E

A

L

L

L

E

S

I

L

V

V

G

G

L

L

W

G

N

D

R

K

R

H

N

D

D

S

L

Y

V

P

K

S

K

N

Y

L

S

S

G

G

M

Q

K

K

R

Q

R

R

L

V

E

A

I

I

A

A

R

R

A

A

L

L

V

A

H

S

Y

N

P

P

K

K

V

V

L

L

F

L

L

C

D

D

E
|
E

P

A

T

T

V

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

M

R

S

S

I

I

W

L

N

E

H

L

I

L

K

K

R

D

L

I

N

N

E

R

R

L

K

G

M

L

T

T

I

I

F

L

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

E

K

A

R

M

I

A

C

D

D

K

C

I

V

A

A

I

V

I

I

D

S

H

N

G

G

K

E

I

L

I

I

E

E

S

Q

G

D

T

T

L

V

A

S

E

E

I

V

M

F

E

S

R

H

T

P

E

K

T

T

E

-

S

P

L

L

E

A

E

Q

S

K

F

F

L

I

K

Q

L

S

T

T

40:46 (vs. 39:45, 86% identical) binding ATP
E166 (= E161) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
38% identity, 79% coverage: 5:214/267 of query aligns to 530:741/1704 of Q99758

query
sites
Q99758

L

I

S

K

V

I

K

K

S

H

L

L

T

S

K

K

K

V

F

F

-

R

-

V

-

G

-

N

D

K

D

D

L

R

T

A

A

A

V

V

K

R

G

D

I

L

S

N

F

L

D

N

V

L

E

Y

T

E

G

G

S

Q

I

I

F

T

A

V

F

L

L

L

G

G

P

H

N
|
N

G

G

A

A

G

G

K

K

S

T

T

T

I

L

K

S

M

M

L
|
L

T

T

T

G

V

L

L

F

R

P

P

P

T

T

S

S

G

G

E

R

I

A

L

Y

I

I

N

S

G

G

Y

Y

N

E

A

I

L

S

K

Q

E

D

Q

M

D

V

K

Q

A

I

R
|
R

S

K

S

S

F

L

G

G

I

L

V

C

F

P

Q

Q

D

H

Y

D

S

I

L

L

D

F

S

D

E

N

L

L

T

T

A

V

Y

A

E

E

N

H

M

L

E

Y

Y

F

H

Y

S

A

V

Q

I

L

Y

K

K

G

V

L

P

S

K

R

A

Q

E

K

R

C

E

P

E

E

R

E

I

V

K

K

T

Q

A

M

L

L

E

H

I

I

V

I

G

G

L

L

W

E

N

D

R

K

R

W

N

N

D

S

L

R

V

S

K

R

K

F

Y

L

S

S

G

G

M

M

K

R

R

R

R

K

L

L

E

S

I

I

A

G

R

I

A

A

L

L

V

I

H

A

Y

G

P

S

K

K

V

V

L

L

F

I

L

L

D

D

E

E

P

P

T

T

V
x
S

G

G

L

M

D

D

P

A

Q

I

T

S

R

R

M

R

S

A

I

I

W

W

N

D

H

L

I

L

K

Q

R

R

L

Q

N

K

E

S

E

D

R

R

K

-

M

-

T

T

I

I

F

V

L

L

T

T

H

H

Y

F

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

I

M

D

A

H

K

G

G

K

E

I

L

N568 (= N39) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (= L50) to P: in SMDP3; uncertain significance
R605 (= R76) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (≠ V164) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance; dbSNP:rs2093663770
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance; dbSNP:rs2093657978
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 88% coverage: 4:239/267 of query aligns to 1:239/240 of 4ymuJ

query
sites
4ymuJ

I

M

L

I

S

F

V

V

K

N

S

D

L

V

T

Y

K

K

K

N

F
|
F

D

G

D

S

L

L

T

E

A
x
V

V

L

K

K

G

G

I

V

S

T

F

L

D

K

V

V

E

N

T

K

G

G

S

E

I

V

F

V

A

V

F

I

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

I

L

K

R

M

C

L

I

T

N

T

L

V

L

L

E

R

E

P

P

T

T

S

K

G

G

E

E

I

V

L

F

I

I

N

D

G

G

Y

V

-

K

-

I

-

N

N

N

A

G

L

K

K

V

E

N

Q

I

D

N

K

K

A

V

R

R

S

Q

S

K

F

V

G

G

I

M

V

V

F

F

Q

Q

D

H

Y

F

S

N

L

L

D

F

S

P

E

H

L

L

T

T

A

A

Y

I

E

E

N

N

M

I

E

T

Y

L

H

A

S

P

V

V

-

K

I

V

Y

K

K

K

V

M

P

N

K

K

A

K

E

E

R

A

E

E

R

L

I

A

K

V

T

D

A

L

L

L

E

A

I

K

V

V

G

G

L

L

W

L

N

D

R

K

N

D

D

Q

L

Y

V

P

K

I

K

K

Y

L

S

S

G

G

M

Q

K

K

R

Q

R

R

L

L

E

A

I

I

A

A

R

R

A

A

L

L

V

A

H

M

Y

Q

P

P

K

E

V

V

L

M

F

L

L

F

D

D

E
|
E

P

P

T

T

V

S

G

A

L

L

D

D

P

P

Q

E

T

M

R

V

M

K

S

E

I

V

W

L

N

N

H

V

I

M

K

K

R

Q

L

L

N

A

E

N

E

E

R

-

K

G

M

M

T

T

I

M

F

V

L

V

T

V

T

T

H
|
H

Y

E

M

M

D

G

E

F

A

A

E

R

A

E

M

V

A

G

D

D

K

R

I

V

A

I

I

F

I

M

D

D

H

D

G

G

K

V

I

I

I

V

E

E

S

E

G

G

T

T

L

P

A

E

E

E

I

I

M

F

E

Y

R

R

T

A

E

K

T

N

E

E

S

R

L

T

E

R

E

E

S

F

F

L

L

S

K

K

L

I

binding adenosine-5'-triphosphate: F11 (= F14), V16 (≠ A19), S36 (≠ N39), G37 (= G40), S38 (≠ A41), G39 (= G42), K40 (= K43), S41 (= S44), T42 (= T45), E162 (= E161), H194 (= H194)
binding magnesium ion: S41 (= S44), E162 (= E161)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
35% identity, 89% coverage: 3:240/267 of query aligns to 1:245/344 of 6cvlD

query
sites
6cvlD

N

H

I

M

L

I

S

K

V

L

K

S

S

N

L

I

T

T

K

K

K

V

F
|
F

D

H

D
x
Q

-

G

-

T

-

R

-

T

L
x
I

T

Q

A

A

V

L

K

N

G

N

I

V

S

S

F

L

D

H

V

V

E

P

T

A

G

G

S

Q

I

I

F

Y

A

G

F

V

L

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

I

I

K

R

M

C

L

V

T

N

T

L

V

L

L

E

R

R

P

P

T

T

S

E

G

G

E

S

I

V

L

L

I

V

N

D

G

G

-

Q

-

E

Y

L

N

T

A

T

L

L

K

S

E

E

Q

S

D

E

-

L

-

T

K

K

A

A

R

R

S

R

S

Q

F

I

G

G

I

M

V

I

F

F

Q

Q

D

H

Y

F

S

N

L

L

D

L

S

S

E

S

L

R

T

T

A

V

Y

F

E

G

N

N

M

V

E

A

Y

L

H

P

S

L

V

E

I

L

Y

D

K

N

V

T

P

P

K

K

A

D

E

E

R

V

E

K

E

R

R

R

I

V

K

T

T

E

A

L

L

L

E

S

I

L

V

V

G

G

L

L

W

G

N

D

R

K

R

H

N

D

D

S

L

Y

V

P

K

S

K
x
N

Y

L

S
|
S

G

G

M
x
Q

K

K

R

Q

R

R

L

V

E

A

I

I

A

A

R

R

A

A

L

L

V

A

H

S

Y

N

P

P

K

K

V

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

V

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

M

R

S

S

I

I

W

L

N

E

H

L

I

L

K

K

R

D

L

I

N

N

E

R

R

L

K

G

M

L

T

T

I

I

F

L

L

L

T

I

T

T

H
|
H

Y

E

M

M

D

D

E

V

A

V

E

K

A

R

M

I

A

C

D

D

K

C

I

V

A

A

I

V

I

I

D

S

H

N

G

G

K

E

I

L

I

I

E

E

S

Q

G

D

T

T

L

V

A

S

E

E

I

V

M

F

E

S

R

H

T

P

E

K

T

T

E

-

S

P

L

L

E

A

E

Q

S

K

F

F

L

I

K

Q

L

S

T

T

binding phosphothiophosphoric acid-adenylate ester: F12 (= F14), Q14 (≠ D16), I19 (≠ L17), S41 (≠ N39), G42 (= G40), A43 (= A41), G44 (= G42), K45 (= K43), S46 (= S44), T47 (= T45), N141 (≠ K135), S143 (= S137), Q146 (≠ M140), H200 (= H194)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
35% identity, 89% coverage: 3:240/267 of query aligns to 1:245/344 of 3tuzC

query
sites
3tuzC

N

H

I

M

L

I

S

K

V

L

K

S

S

N

L

I

T

T

K

K

K

V

F
|
F

D

H

D
x
Q

-

G

-

T

-

R

-

T

L

I

T

Q

A

A

V

L

K

N

G

N

I

V

S

S

F

L

D

H

V

V

E

P

T

A

G

G

S

Q

I

I

F

Y

A

G

F

V

L

I

G

G

P

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

I

I

K

R

M

C

L

V

T

N

T

L

V

L

L

E

R

R

P

P

T

T

S

E

G

G

E

S

I

V

L

L

I

V

N

D

G

G

-

Q

-

E

Y

L

N

T

A

T

L

L

K

S

E

E

Q

S

D

E

-

L

-

T

K

K

A

A

R

R

S

R

S

Q

F

I

G

G

I

M

V

I

F

F

Q

Q

D

H

Y

F

S

N

L

L

D

L

S

S

E

S

L

R

T

T

A

V

Y

F

E

G

N

N

M

V

E

A

Y

L

H

P

S

L

V

E

I

L

Y

D

K

N

V

T

P

P

K

K

A

D

E

E

R

V

E

K

E

R

R

R

I

V

K

T

T

E

A

L

L

L

E

S

I

L

V

V

G

G

L

L

W

G

N

D

R

K

R

H

N

D

D

S

L

Y

V

P

K

S

K

N

Y

L

S

S

G

G

M

Q

K

K

R

Q

R

R

L

V

E

A

I

I

A

A

R

R

A

A

L

L

V

A

H

S

Y

N

P

P

K

K

V

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

V

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

M

R

S

S

I

I

W

L

N

E

H

L

I

L

K

K

R

D

L

I

N

N

E

R

R

L

K

G

M

L

T

T

I

I

F

L

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

E

K

A

R

M

I

A

C

D

D

K

C

I

V

A

A

I

V

I

I

D

S

H

N

G

G

K

E

I

L

I

I

E

E

S

Q

G

D

T

T

L

V

A

S

E

E

I

V

M

F

E

S

R

H

T

P

E

K

T

T

E

-

S

P

L

L

E

A

E

Q

S

K

F

F

L

I

K

Q

L

S

T

T

binding adenosine-5'-diphosphate: F12 (= F14), Q14 (≠ D16), G42 (= G40), G44 (= G42), K45 (= K43), S46 (= S44), T47 (= T45)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
35% identity, 89% coverage: 3:240/267 of query aligns to 1:245/344 of 3tuiC

query
sites
3tuiC

N

H

I

M

L

I

S

K

V

L

K

S

S

N

L

I

T

T

K

K

K

V

F
|
F

D

H

D

Q

-

G

-

T

-

R

-

T

L

I

T

Q

A

A

V

L

K

N

G

N

I

V

S

S

F

L

D

H

V

V

E

P

T

A

G

G

S

Q

I

I

F

Y

A

G

F

V

L

I

G

G

P

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

I

I

K

R

M

C

L

V

T

N

T

L

V

L

L

E

R

R

P

P

T

T

S

E

G

G

E

S

I

V

L

L

I

V

N

D

G

G

-

Q

-

E

Y

L

N

T

A

T

L

L

K

S

E

E

Q

S

D

E

-

L

-

T

K

K

A

A

R

R

S

R

S

Q

F

I

G

G

I

M

V

I

F

F

Q

Q

D

H

Y

F

S

N

L

L

D

L

S

S

E

S

L

R

T

T

A

V

Y

F

E

G

N

N

M

V

E

A

Y

L

H

P

S

L

V

E

I

L

Y

D

K

N

V

T

P

P

K

K

A

D

E

E

R

V

E

K

E

R

R

R

I

V

K

T

T

E

A

L

L

L

E

S

I

L

V

V

G

G

L

L

W

G

N

D

R

K

R

H

N

D

D

S

L

Y

V

P

K

S

K

N

Y

L

S

S

G

G

M

Q

K

K

R

Q

R

R

L

V

E

A

I

I

A

A

R

R

A

A

L

L

V

A

H

S

Y

N

P

P

K

K

V

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

V

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

M

R

S

S

I

I

W

L

N

E

H

L

I

L

K

K

R

D

L

I

N

N

E

R

R

L

K

G

M

L

T

T

I

I

F

L

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

E

K

A

R

M

I

A

C

D

D

K

C

I

V

A

A

I

V

I

I

D

S

H

N

G

G

K

E

I

L

I

I

E

E

S

Q

G

D

T

T

L

V

A

S

E

E

I

V

M

F

E

S

R

H

T

P

E

K

T

T

E

-

S

P

L

L

E

A

E

Q

S

K

F

F

L

I

K

Q

L

S

T

T

binding adenosine-5'-diphosphate: F12 (= F14), G42 (= G40), A43 (= A41), G44 (= G42), K45 (= K43), S46 (= S44), T47 (= T45)

7lkzA Structure of atp-bound human abca4 (see paper)
40% identity, 80% coverage: 7:219/267 of query aligns to 711:923/1920 of 7lkzA

query
sites
7lkzA

V

V

K

K

S

N

L

L

T

V

K

K

K

I

F
|
F

D

E

D

P

L

C

-

G

-

R

T

P

A
|
A

V

V

K

D

G

R

I

L

S

N

F

I

D

T

V

F

E

Y

T

E

G

N

S

Q

I

I

F

T

A

A

F

F

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T

T

I

L

K

S

M

I

L

L

T

T

T

G

V

L

L

L

R

P

P

P

T

T

S

S

G

G

E

T

I

V

L

L

I

V

N

G

G

G

Y

R

N

D

A

I

L

E

K

T

E

S

Q

L

D

D

K

A

A

V

R

R

S

Q

S

S

F

L

G

G

I

M

V

C

F

P

Q
|
Q

D

H

Y

N

S

I

L

L

D

F

S

H

E

H

L

L

T

T

A

V

Y

A

E

E

N

H

M

M

E

L

Y

F

H

Y

S

A

V

Q

I

L

Y

K

K

G

V

K

P

S

K

Q

A

E

E

E

R

A

E

Q

E

L

R

E

I

M

K

E

T

A

A

M

L

L

E

E

I

D

V

T

G

G

L

L

W

H

N

H

R
x
K

R

R

N

N

D

E

L

E

V

A

K

Q

K
x
D

Y

L

S
|
S

G

G

M

M

K

Q

R

R

R

K

L

L

E

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

Y

D

P

A

K

K

V

V

L

V

F

I

L

L

D

D

E

Q

P

P

T

T

V

S

G

G

L

V

D

D

P

P

Q

Y

T

S

R

R

M

R

S

S

I

I

W

W

N

D

H

L

I

L

K

L

R

K

L

Y

N

R

E

S

E

G

R

R

K

-

M

-

T

T

I

I

F

I

L

M

T

S

T

T

H

H

Y

H

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

D

A

H

Q

G

G

K

R

I

L

I

Y

E

C

S

S

G

G

T

T

binding adenosine-5'-triphosphate: F718 (= F14), A725 (= A19), N745 (= N39), G746 (= G40), G748 (= G42), K749 (= K43), T750 (≠ S44), K834 (≠ R128), D841 (≠ K135), S843 (= S137)
binding magnesium ion: T750 (≠ S44), Q790 (= Q84)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 22, 465, 566, 567, 568, 572, 592, 593, 595, 1076, 1355, 1362, 1470, 1471
binding adenosine-5'-triphosphate: 1619, 1646, 1647, 1649, 1650, 1651, 1652, 1742, 1744, 1745

7e7qA Cryo-em structure of human abca4 in atp-bound state (see paper)
40% identity, 80% coverage: 7:219/267 of query aligns to 794:1006/1958 of 7e7qA

query
sites
7e7qA

V

V

K

K

S

N

L

L

T

V

K

K

K

I

F
|
F

D

E

D

P

L
x
C

-

G

-

R

T

P

A
|
A

V

V

K

D

G

R

I

L

S

N

F

I

D

T

V

F

E

Y

T

E

G

N

S

Q

I

I

F

T

A

A

F

F

L

L

G

G

P

H

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

I

L

K

S

M

I

L

L

T

T

T

G

V

L

L

L

R

P

P

P

T

T

S

S

G

G

E

T

I

V

L

L

I

V

N

G

G

G

Y

R

N

D

A

I

L

E

K

T

E

S

Q

L

D

D

K

A

A

V

R

R

S

Q

S

S

F

L

G

G

I

M

V

C

F

P

Q
|
Q

D

H

Y

N

S

I

L

L

D

F

S

H

E

H

L

L

T

T

A

V

Y

A

E

E

N

H

M

M

E

L

Y

F

H

Y

S

A

V

Q

I

L

Y

K

K

G

V

K

P

S

K

Q

A

E

E

E

R

A

E

Q

E

L

R

E

I

M

K

E

T

A

A

M

L

L

E

E

I

D

V

T

G

G

L

L

W

H

N

H

R

K

R

R

N

N

D

E

L

E

V

A

K

Q

K

D

Y

L

S
|
S

G

G

G
|
G

M

M

K

Q

R

R

R

K

L

L

E

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

Y

D

P

A

K

K

V

V

L

V

F

I

L

L

D

D

E

Q

P

P

T

T

V

S

G

G

L

V

D

D

P

P

Q

Y

T

S

R

R

M

R

S

S

I

I

W

W

N

D

H

L

I

L

K

L

R

K

L

Y

N

R

E

S

E

G

R

R

K

-

M

-

T

T

I

I

F

I

L

M

T

S

T

T

H

H

Y

H

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

D

A

H

Q

G

G

K

R

I

L

I

Y

E

C

S

S

G

G

T

T

binding adenosine-5'-triphosphate: F801 (= F14), C804 (≠ L17), A808 (= A19), N828 (= N39), G831 (= G42), K832 (= K43), T833 (≠ S44), T834 (= T45), Q873 (= Q84), S926 (= S137), G928 (= G139)
binding magnesium ion: T833 (≠ S44), Q873 (= Q84)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1671, 1698, 1701, 1702, 1703, 1704, 1743, 1794, 1796
binding magnesium ion: 1703, 1743

7w02A Cryo-em structure of atp-bound abca3 (see paper)
38% identity, 79% coverage: 5:214/267 of query aligns to 495:706/1566 of 7w02A

query
sites
7w02A

L

I

S

K

V

I

K

K

S

H

L

L

T

S

K

K

K

V

F
|
F

-

R

-

V

-

G

-

N

D

K

D

D

L

R

T

A

A

A

V

V

K

R

G

D

I

L

S

N

F

L

D

N

V

L

E

Y

T

E

G

G

S

Q

I

I

F

T

A

V

F

L

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

I

L

K

S

M

M

L

L

T

T

T

G

V

L

L

F

R

P

P

P

T

T

S

S

G

G

E

R

I

A

L

Y

I

I

N

S

G

G

Y

Y

N

E

A

I

L

S

K

Q

E

D

Q

M

D

V

K

Q

A

I

R

R

S

K

S

S

F

L

G

G

I

L

V

C

F

P

Q
|
Q

D

H

Y

D

S

I

L

L

D

F

S

D

E

N

L

L

T

T

A

V

Y

A

E

E

N

H

M

L

E

Y

Y

F

H

Y

S

A

V

Q

I

L

Y

K

K

G

V

L

P

S

K

R

A

Q

E

K

R

C

E

P

E

E

R

E

I

V

K

K

T

Q

A

M

L

L

E

H

I

I

V

I

G

G

L

L

W

E

N

D

R

K

R

W

N

N

D

S

L

R

V

S

K

R

K

F

Y
x
L

S
|
S

G

G

M

M

K

R

R

R

R

K

L

L

E

S

I

I

A

G

R

I

A

A

L

L

V

I

H

A

Y

G

P

S

K

K

V

V

L

L

F

I

L

L

D

D

E

Q

P

P

T

T

V

S

G

G

L

M

D

D

P

A

Q

I

T

S

R

R

M

R

S

A

I

I

W

W

N

D

H

L

I

L

K

Q

R

R

L

Q

N

K

E

S

E

D

R

R

K

-

M

-

T

T

I

I

F

V

L

L

T

T

H

H

Y

F

M

M

D

D

E

E

A

A

E

D

A

L

M

L

A

G

D

D

K

R

I

I

A

A

I

I

I

M

D

A

H

K

G

G

K

E

I

L

binding adenosine-5'-triphosphate: F504 (= F14), N533 (= N39), G534 (= G40), G536 (= G42), K537 (= K43), T538 (≠ S44), T539 (= T45), Q578 (= Q84), L630 (≠ Y136), S631 (= S137)
binding magnesium ion: T538 (≠ S44), Q578 (= Q84)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1277, 1305, 1306, 1307, 1308, 1309, 1310, 1344, 1395, 1398
binding magnesium ion: 1310, 1344

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
40% identity, 81% coverage: 5:219/267 of query aligns to 929:1143/2273 of P78363

query
sites
P78363

L
x
V

S
x
C

V
|
V

K
|
K

S
x
N

L
|
L

T
x
V

K
|
K

K
x
I

F
|
F

D
x
E

D
x
P

L
x
C

-
x
G

-
x
R

T
x
P

A
|
A

V
|
V

K
x
D

G
x
R

I
x
L

S
x
N

F
x
I

D
x
T

V
x
F

E
x
Y

T
x
E

G
x
N

S
x
Q

I
|
I

F
x
T

A
|
A

F
|
F

L
|
L

G
|
G

P
x
H

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
x
T

T
|
T

I
x
L

K
x
S

M
x
I

L
|
L

T
|
T

T
x
G

V
x
L

L
|
L

R
x
P

P
|
P

T
|
T

S
|
S

G
|
G

E
x
T

I
x
V

L
|
L

I
x
V

N
x
G

G
|
G

Y
x
R

N
x
D

A
x
I

L
x
E

K
x
T