SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011022159.1 NCBI__GCF_000007345.1:WP_011022159.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 76% coverage: 44:182/184 of query aligns to 47:184/203 of P07464

query
sites
P07464

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S71 (≠ A68) binding
N85 (≠ G80) binding in other chain; binding in other chain
D93 (≠ Q88) binding
H115 (= H110) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S140) binding in other chain
A160 (= A158) binding in other chain
TK 165:166 (≠ TR 163:164) binding
R180 (≠ K178) binding
R183 (≠ K181) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
33% identity, 76% coverage: 44:182/184 of query aligns to 46:183/200 of 1krrA

query
sites
1krrA

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binding acetyl coenzyme *a: N84 (≠ G80), I103 (≠ M99), A104 (≠ G100), P105 (≠ R101), T112 (= T108), H114 (= H110), G140 (= G139), S141 (= S140), N146 (≠ L145), G158 (= G157), A159 (= A158), A174 (≠ G173), P177 (= P176), R182 (≠ K181)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
33% identity, 76% coverage: 44:182/184 of query aligns to 46:183/201 of 1krvA

query
sites
1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ A68), Y82 (= Y78), N84 (≠ G80), V90 (≠ L86), D92 (≠ Q88), M126 (≠ K125)
binding coenzyme a: A104 (≠ G100), S110 (≠ M106), T112 (= T108), W138 (= W137), G140 (= G139), S141 (= S140), N146 (≠ L145), A159 (= A158), P177 (= P176), R179 (≠ K178), R182 (≠ K181)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
33% identity, 76% coverage: 44:182/184 of query aligns to 46:183/201 of 1kruA

query
sites
1kruA

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binding coenzyme a: H114 (= H110), W138 (= W137), G140 (= G139), S141 (= S140), G158 (= G157), A159 (= A158), P177 (= P176), R182 (≠ K181)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ N60), S70 (≠ A68), Y82 (= Y78), Y82 (= Y78), N84 (≠ G80), V90 (≠ L86), D92 (≠ Q88), L102 (≠ M98), H114 (= H110), M126 (≠ K125)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
39% identity, 76% coverage: 44:182/184 of query aligns to 46:183/186 of 4isxA

query
sites
4isxA

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binding acetyl coenzyme *a: N84 (= N77), A104 (≠ G100), W138 (= W137), G140 (= G139), G141 (≠ S140), A159 (= A158), V174 (≠ G173), K182 (= K181)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
34% identity, 79% coverage: 39:184/184 of query aligns to 41:185/185 of 3nz2J

query
sites
3nz2J

L

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binding acetyl coenzyme *a: G104 (= G100), Y110 (≠ M106), H114 (= H110), W138 (= W137), G140 (= G139), A158 (≠ G157), A159 (= A158), N164 (≠ T163), G174 (= G173), T176 (= T175), P177 (= P176), R182 (≠ K181)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
34% identity, 79% coverage: 39:184/184 of query aligns to 31:175/176 of 3ectA

query
sites
3ectA

L

L

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binding calcium ion: G138 (= G147), D156 (= D165)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
34% identity, 79% coverage: 39:184/184 of query aligns to 38:182/183 of 3nz2C

query
sites
3nz2C

L

L

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H

E

-

T

-

S

-

D

-

A

-

S

S

I

L

P

D

M

Y

R

R

Y

R

Q

R

G

Q

G

A

K

W

E

E

-

T

-

I

V

C

S

K

P

P

V

I

I

V

I

I

G

E

D

D

V

V

W

W

I

I

G
|
G

S

G

R

N

V

V

I

V

I

I

L

N

P

Q

G

G

V

V

R

T

I

I

G

G

T

A

G

R

S

S

I

V

G
x
A

A
|
A

G
x
N

A

S

V

V

T
x
N

R

Q

D

D

V

V

E

P

P

P

Y

D

S

T

V

L

V

V

G
|
G

G

G

T
|
T

P
|
P

A

A

K

R

I

I

I
x
L

K
x
R

K

S

R

L

K

K

binding acetyl coenzyme *a: Y107 (≠ M106), H111 (= H110), G137 (= G139), A155 (≠ G157), A156 (= A158), N161 (≠ T163), G171 (= G173), T173 (= T175), P174 (= P176), L178 (≠ I180), R179 (≠ K181)
binding magnesium ion: N157 (≠ G159), T173 (= T175)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
39% identity, 50% coverage: 91:182/184 of query aligns to 95:183/190 of 5u2kA

query
sites
5u2kA

V

I

S

T

I

I

G

G

N

D

D

N

V

V

M

F

M

I

G

G

R

P

D

N

V

C

I

G

I

F

M
x
Y

T

T

T
x
A

S

T

H
|
H

E

P

T

-

S

-

D

-

A

L

S

N

I

F

P

H

M

H

R

R

Y

N

Q

E

G

G

G

F

K

E

E

K

V

A

S

G

P

P

V

I

I

H

I

I

G

G

D

S

D

N

V

T

W

W

I

F

G

G

S

G

R

H

V

V

I

A

I

V

L
|
L

P

P

G

G

V

V

R

T

I

I

G

G

T

E

G

G

S

S

I

V

I

I

G

G

A

A

G

G

A

S

V

V

T
|
T

R
x
K

D

D

V

I

E

P

P

P

Y

H

S

S

V

L

V

A

G

V

G

G

T

N

P

P

A

C

K

K

I

V

K

R

K

K

binding coenzyme a: Y110 (≠ M106), A112 (≠ T108), H114 (= H110), L146 (= L145), T164 (= T163), K165 (≠ R164)

Sites not aligning to the query:

binding coenzyme a: 90

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
59% identity, 29% coverage: 130:183/184 of query aligns to 110:163/209 of 8j40A

query
sites
8j40A

V

T

I

V

I

I

G

G

D

N

D

D

V

V

W
|
W

I

I

G

G

S

S

R

E

V

A

I

M

I

I

L

M

P

P

G

G

V

I

R

K

I

I

G

G

T

D

G

G

S

A

I
x
V

I

I

G

G

A
x
S

G

R

A

S

V

L

V

V

T

T

R

K

D

D

V

V

E

E

P

P

Y

Y

S

A

V

I

G

G

T

N

P

P

A

A

K

K

I

Q

I

I

K

K

R

R

binding chloramphenicol: W117 (= W137), V135 (≠ I155), S138 (≠ A158)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 90, 187, 190, 191, 202, 203, 206

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
39% identity, 58% coverage: 77:183/184 of query aligns to 52:166/212 of 4husA

query
sites
4husA

N

H

Y

Y

S

E

G

V

I

I

G

G

I

D

N

K

S

L

L

I

V

I

Q

G

R

R

N

F

V

C

S

S

I

I

G

G

N

P

D

-

V

-

M

-

G

G

R

T

D

T

V

F

I

I

I

M

M

N

T

G

T

A

S

N

H

H

E

R

T

M

S

D

D

G

A

S

S

T

I

Y

P

P

M

F

R

H

Y

L

-

F

-

R

Q

M

G

G

G

W

K

E

E

K

V

Y

S

M

P

P

V

S

I

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

-

I

-

E

I

I

G

G

D

N

D

D

V

V

W

W

I

I

G

G

S

R

R

D

V

V

I

T

I

I

L

M

P

P

G

G

V

V

R

K

I

I

G

G

T

D

G

G

S

A

I

I

G

A

A

A

G

E

A

A

V

V

T

T

R

K

D

N

V

V

E

A

P

P

Y

Y

S

S

V

I

V

V

G

G

T

N

P

P

A

L

K

K

I

F

I

I

K

R

K

K

R

R

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
39% identity, 58% coverage: 77:183/184 of query aligns to 52:166/211 of 4hurA

query
sites
4hurA

N

H

Y

Y

S

E

G

V

I

I

G

G

I

D

N

K

S

L

L

I

V

I

Q

G

R

R

N

F

V

C

S
|
S

I
|
I

G
|
G

N

P

D

-

V

-

M

-

G

G

R

T

D

T

V

F

I

I

I

M

M

N

T

G

T
x
A

S
x
N

H

H

E

R

T

M

S

D

D

G

A

S

S

T

I

Y

P

P

M

F

R

H

Y

L

-

F

-

R

Q

M

G

G

G

W

K

E

E

K

V

Y

S

M

P

P

V

S

I

L

-

K

-

D

-

L

-

P

-

L

-
x
K

-

G

-

D

-

I

-

E

I

I

G

G

D

N

D

D

V

V

W
|
W

I
|
I

G
|
G

S
x
R

R

D

V

V

I

T

I

I

L
x
M

P

P

G

G

V

V

R

K

I

I

G

G

T

D

G

G

S

A

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T
|
T

R

K

D

N

V

V

E

A

P

P

Y

Y

S

S

V

I

V

V

G
|
G

G

G

T

N

P
|
P

A

L

K

K

I

F

I
|
I

K
x
R

K

K

R

R

binding acetyl coenzyme *a: S67 (= S92), I68 (= I93), G69 (= G94), A79 (≠ T108), N80 (≠ S109), K110 (vs. gap), W120 (= W137), I121 (= I138), G122 (= G139), R123 (≠ S140), M128 (≠ L145), A140 (≠ G157), A141 (= A158), T146 (= T163), G156 (= G173), P159 (= P176), I163 (= I180), R164 (≠ K181)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
39% identity, 58% coverage: 77:183/184 of query aligns to 52:166/207 of 6x3cA

query
sites
6x3cA

N

H

Y
|
Y

S

E

G

V

I

I

G

G

I

D

N

K

S

L

L

I

V

I

Q

G

R

R

N

F

V

C

S

S

I

I

G
|
G

N

P

D

-

V

-

M

-

G

G

R

T

D

T

V

F

I

I

I

M

M

N

T
x
G

T

A

S
x
N

H
|
H

E

R

T

M

S

D

D

G

A

S

S

T

I

Y

P

P

M

F

R
x
H

Y
x
L

-

F

-

R

Q

M

G

G

G

W

K

E

E

K

V

Y

S
x
M

P
|
P

V

S

I
x
L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

-

I

-

E

I

I

G

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

S
x
R

R

D

V

V

I

T

I

I

L
x
M

P

P

G

G

V

V

R

K

I

I

G

G

T

D

G

G

S

A

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T

T

R

K

D

N

V

V

E

A

P

P

Y

Y

S

S

V

I

V

V

G

G

T

N

P

P

A

L

K

K

I

F

I

I

K

R

K

K

R

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (= Y78), G78 (≠ T107), N80 (≠ S109), H81 (= H110), H91 (≠ R120), L92 (≠ Y121), M101 (≠ S128), P102 (= P129), L104 (≠ I131)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G94), N80 (≠ S109), H81 (= H110), W120 (= W137), G122 (= G139), R123 (≠ S140), M128 (≠ L145), A140 (≠ G157), A141 (= A158)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
39% identity, 58% coverage: 77:183/184 of query aligns to 52:166/203 of 6x3cE

query
sites
6x3cE

N

H

Y
|
Y

S

E

G

V

I

I

G

G

I

D

N

K

S

L

L

I

V

I

Q

G

R

R

N

F

V

C

S

S

I

I

G
|
G

N

P

D

-

V

-

M

-

G

G

R

T

D

T

V

F

I

I

I

M

M

N

T
x
G

T

A

S
x
N

H
|
H

E

R

T
x
M

S

D

D

G

A

S

S

T

I

Y

P

P

M

F

R
x
H

Y
x
L

-

F

-

R

Q

M

G

G

G

W

K

E

E

K

V

Y

S
x
M

P
|
P

V

S

I

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

-

I

-

E

I

I

G

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

S

R

R

D

V

V

I

T

I

I

L

M

P

P

G

G

V

V

R

K

I

I

G

G

T

D

G

G

S

A

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T

T

R

K

D

N

V

V

E

A

P

P

Y

Y

S

S

V

I

V

V

G

G

T
x
N

P
|
P

A

L

K

K

I

F

I

I

K

R

K

K

R

R

binding magnesium ion: N158 (≠ T175), P159 (= P176)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (= Y78), G78 (≠ T107), N80 (≠ S109), H81 (= H110), H91 (≠ R120), L92 (≠ Y121), M101 (≠ S128), P102 (= P129)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G94), H81 (= H110), M83 (≠ T112), W120 (= W137), G122 (= G139), A140 (≠ G157), A141 (= A158)

Sites not aligning to the query:

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: 51

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
39% identity, 58% coverage: 77:183/184 of query aligns to 52:166/206 of 6x3jA

query
sites
6x3jA

N

H

Y
|
Y

S

E

G
x
V

I

I

G

G

I

D

N

K

S

L

L

I

V

I

Q

G

R

R

N

F

V

C

S

S

I

I

G
|
G

N

P

D

-

V

-

M

-

G

G

R

T

D

T

V

F

I

I

I

M

M

N

T

G

T
x
A

S

N

H
|
H

E

R

T

M

S

D

D

G

A

S

S

T

I

Y

P

P

M

F

R
x
H

Y
x
L

-

F

-

R

Q

M

G

G

G

W

K

E

E

K

V

Y

S
x
M

P
|
P

V

S

I

L

-

K

-

D

-
x
L

-

P

-

L

-

K

-

G

-

D

-

I

-

E

I

I

G

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

S

R

R

D

V

V

I

T

I

I

L

M

P

P

G

G

V

V

R

K

I

I

G

G

T

D

G

G

S

A

I

I

G

A

A

A

G

E

A

A

V

V

T

T

R

K

D

N

V

V

E

A

P

P

Y

Y

S

S

V

I

V

V

G

G

T

N

P

P

A

L

K

K

I

F

I

I

K

R

K

K

R

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y53 (= Y78), V55 (≠ G80), H91 (≠ R120), L92 (≠ Y121), M101 (≠ S128), P102 (= P129), L107 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G94), A79 (≠ T108), H81 (= H110), W120 (= W137), G122 (= G139)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
52% identity, 34% coverage: 122:183/184 of query aligns to 101:162/208 of 2xatA

query
sites
2xatA

Q

N

G

G

G

Y

K

Q

E

P

V

A

S

G

P

D

V

T

I

L

I

I

G

G

D

H

D

E

V

V

W

W

I

I

G
|
G

S
x
T

R

E

V

A

I

M

I

F

L

M

P

P

G

G

V

V

R

R

I

V

G

G

T

H

G

G

S

A

I

I

G
|
G

A
x
S

G

R

A

A

V

L

V

V

T

T

R

G

D

D

V

V

E

E

P

P

Y

Y

S

A

V

I

V

V

G
|
G

T

N

P

P

A

A

K

R

I

T

I
|
I

K

R

K

K

R

R

binding desulfo-coenzyme a: G118 (= G139), T119 (≠ S140), G136 (= G157), S137 (≠ A158), G152 (= G173), G153 (= G174), I159 (= I180)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
43% identity, 51% coverage: 90:182/184 of query aligns to 189:279/307 of A1ADJ6

query
sites
A1ADJ6

N

N

V

V

S

T

I

I

G

G

N

H

D

D

V

C

M

M

M

I

G

A

R

R

D

D

V

V

I

I

I

L

M

R

T

A

T

S

S

D

-

G

H
|
H

E

P

T

I

S

F

D

D

A

I

S

H

I

S

P

K

M

K

R

R

Y

I

Q

N

G

W

G

A

K

K

E

D

V

I

S

-

P

-

V

-

I

I

G

S

D

S

D

Y

V

V

W
|
W

I

V

G

G

S

R

R

N

V

V

I

S

I

I

L

M

P

K

G

G

V

V

R

S

I

V

G

G

T

S

G

G

S

S

I

V

I

I

G

G

A

Y

G

G

A

S

V

I

V

V

T

T

R

K

D

D

V

V

E

P

P

S

Y

M

S

C

V

A

G

A

G

G

T

N

P

P

A

A

K

K

I

I

K

K

K

R

H210 (= H110) mutation to A: Loss of activity.
W234 (= W137) mutation to A: Loss of activity.

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
47% identity, 49% coverage: 91:181/184 of query aligns to 97:184/188 of 3igjC

query
sites
3igjC

V

V

S

R

I

I

G

G

N

D

D

H

V

C

M

M

M

F

G
x
A

R

P

D

G

V

V

I

H

I

I

M
x
Y

T

T

T
x
A

S

T

H
|
H

E

P

T

L

S

H

D

-

A

-

S

-

I

-

P

P

M

V

R

E

Y

R

Q

N

G

S

G

G

K

K

E

E

V

Y

-

G

S

K

P

P

V

V

I

K

I

I

G

G

D

N

V

V

W

W

I

V

G
|
G

S

G

R

G

V

A

I

I

L
x
N

P

P

G

G

V

V

R

S

I

I

G

G

T

D

G

N

S

A

I

V

I

I

G
x
A

A
x
S

G

G

A

A

V

V

T
|
T

R

K

D

D

V

V

E

P

P

N

Y

N

S

V

V

V

G

G

T
x
N

P

P

A

A

K

K

I

V

I

I

K

K

binding acetyl coenzyme *a: A106 (≠ G100), Y112 (≠ M106), A114 (≠ T108), H116 (= H110), G142 (= G139), N148 (≠ L145), A160 (≠ G157), S161 (≠ A158), T166 (= T163), N178 (≠ T175)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
34% identity, 76% coverage: 45:183/184 of query aligns to 32:162/206 of 6u9cA

query
sites
6u9cA

G

G

F

Y

L

Y

A

H

S

G

K

H

I

S

F

F

D

D

E

D

C

C

G

V

V

R

G

Y

V

L

N

H

L

P

E

E

K

R

G

D

A

-

Y

-

I

-

A

-

D

D

G

V

K

D

F

K

I

L

R

V

V

I

G

G

N

S

Y

F

S

C

G

S

I

I

G

G

I

S

N

G

S

A

L

V

V

F

Q

-

R

-

N

-

V

M

S

M

I

A

G

G

N

N

D

Q

V

G

M

H

M

R

G

S

R

D

D

W

V

I

I

S

I

T

M

F

T

P

T

F

S

F

H

Y

E

Q

T

D

S

N

D

D

A

N

S

F

I

A

P

D

M

A

R

R

Y

-

Q

D

G

G

G

F

K

T

E

R

V

S

S

G

P

D

V

T

I

I

G

G

D

H

D

D

V

V

W
|
W

I

I

G

G

S

T

R

E

V

A

I

M

I

I

L

M

P

P

G

G

V

V

R

K

I

I

G

G

T

H

G

G

S

A

I

I

G
x
A

A

S

G

R

A

S

V

V

T

T

R

K

D

D

V

V

E

A

P

P

Y

Y

S

E

V

V

G

G

G

S

T

N

P

P

A

A

K

K

I

H

I
|
I

K

K

K

F

R

R

binding acetyl coenzyme *a: W116 (= W137), A136 (≠ G157), I159 (= I180)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
34% identity, 76% coverage: 45:183/184 of query aligns to 35:165/210 of 6pubA

query
sites
6pubA

G

G

F
x
Y

L
x
Y

A

H

S

G

K

H

I

S

F

F

D

D

E

D

C

C

G

V

V

R

G

Y

V

L

N

H

L

P

E

E

K

R

G

D

A

-

Y

-

I

-

A

-

D

D

G

V

K

D

F

K

I

L

R

V

V

I

G

G

N

S

Y

F

S

C

G

S

I

I

G

G

I

S

N

G

S

A

L

V

V

F

Q

-

R

-

N

-

V

M

S

M

I

A

G

G

N

N

D

Q

V

G

M

H

M

R

G

S

R

D

D

W

V

I

I

S

I

T

M

F

T

P

T

F

S

F

H

Y

E

Q

T

D

S

N

D

D

A

N

S

F

I

A

P

D

M

A

R

R

Y

-

Q

D

G

G

G

F

K

T

E

R

V

S

S

G

P

D

V

T

I

I

G

G

D

H

D

D

V

V

W

W

I

I

G

G

S

T

R

E

V

A

I

M

I

I

L

M

P

P

G

G

V

V

R

K

I

I

G

G

T

H

G

G

S

A

I

I

G

A

A

S

G

R

A

S

V

V

T

T

R

K

D

D

V

V

E

A

P

P

Y

Y

S

E

V

V

G

G

G

S

T

N

P

P

A

A

K

K

I

H

I

I

K

K

K

F

R

R

binding crystal violet: Y36 (≠ F46), Y37 (≠ L47)

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11

Query Sequence

>WP_011022159.1 NCBI__GCF_000007345.1:WP_011022159.1
MKSKVINYLKSLEKYTALIFYYSIARHLPPSDEPQSLKLSKPIRGFLASKIFDECGVGVN
LEKGAYIADGKFIRVGNYSGIGINSLVQRNVSIGNDVMMGRDVIIMTTSHETSDASIPMR
YQGGKEVSPVIIGDDVWIGSRVIILPGVRIGTGSIIGAGAVVTRDVEPYSVVGGTPAKII
KKRK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory