SitesBLAST
Comparing WP_011023202.1 NCBI__GCF_000007345.1:WP_011023202.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 17 hits to proteins with known functional sites (download)
3vpdA Lysx from thermus thermophilus complexed with amp-pnp (see paper)
40% identity, 62% coverage: 87:286/324 of query aligns to 67:259/281 of 3vpdA
- binding phosphoaminophosphonic acid-adenylate ester: V127 (= V146), K129 (= K148), W135 (≠ K154), G136 (= G155), L139 (≠ I158), Q169 (= Q195), V172 (≠ I198), K174 (≠ N200), D178 (= D204), R194 (= R220), W199 (= W227), T201 (≠ N229), F239 (≠ I267), E249 (= E277)
- binding butanoic acid: F256 (≠ D283), K257 (= K284), N258 (≠ S285), S259 (≠ T286)
5k2mI Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
34% identity, 78% coverage: 35:288/324 of query aligns to 10:253/273 of 5k2mI
- binding adenosine-5'-diphosphate: K83 (= K109), V120 (≠ I147), K122 (= K148), S127 (≠ Y153), L132 (≠ I158), Q162 (= Q195), V165 (≠ I198), K167 (≠ N200), D171 (= D204), W192 (= W227), I193 (≠ V228), T194 (≠ N229), N195 (= N230), F231 (≠ I267), N240 (= N276), E241 (= E277)
- binding 2-aminohexanedioic acid: R173 (= R206), N195 (= N230), T196 (≠ L231), A197 (≠ S232), E247 (= E282), F248 (≠ D283), N250 (≠ S285)
- binding : V51 (= V71), S52 (≠ G72), H53 (≠ A73), F54 (= F76), F125 (= F151), W128 (≠ K154), R130 (≠ K156), N153 (vs. gap), Y156 (vs. gap), N245 (≠ K280)
5k2mG Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
34% identity, 78% coverage: 35:288/324 of query aligns to 10:253/273 of 5k2mG
- binding adenosine-5'-diphosphate: K83 (= K109), V120 (≠ I147), K122 (= K148), S127 (≠ Y153), G129 (= G155), Q162 (= Q195), V165 (≠ I198), K167 (≠ N200), D171 (= D204), W192 (= W227), T194 (≠ N229), N195 (= N230), F231 (≠ I267), N240 (= N276), E241 (= E277)
- binding magnesium ion: D229 (= D265), E241 (= E277)
- binding : V51 (= V71), S52 (≠ G72), H53 (≠ A73), F54 (= F76), F125 (= F151), S127 (≠ Y153), W128 (≠ K154), R130 (≠ K156), Y156 (vs. gap), R173 (= R206), R187 (= R220), T196 (≠ L231), A197 (≠ S232), N243 (= N278), P244 (≠ K279), N245 (≠ K280), E247 (= E282), F248 (≠ D283), N250 (≠ S285), A251 (≠ T286)
7qyrD Crystal structure of rimk from pseudomonas aeruginosa pao1 (see paper)
29% identity, 98% coverage: 3:321/324 of query aligns to 2:287/293 of 7qyrD
- binding adenosine-5'-diphosphate: V139 (= V146), Y179 (≠ F197), I180 (= I198), D187 (= D204), R211 (≠ V228), S212 (≠ N229), L250 (≠ I267), M259 (≠ L293)
- binding : S7 (≠ I8), L12 (≠ D13), S14 (≠ T15), R64 (= R69), R189 (= R206), N262 (= N296), S264 (≠ T298), G266 (≠ S300)
1uc9A Crystal structure of a lysine biosynthesis enzyme, lysx, from thermus thermophilus hb8 (see paper)
35% identity, 62% coverage: 87:286/324 of query aligns to 67:235/256 of 1uc9A
Q4JAP9 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
26% identity, 95% coverage: 14:321/324 of query aligns to 3:271/276 of Q4JAP9
- NT 253:254 (≠ GI 303:304) mutation to GF: Alters substrate specificity, so that glutamate is preferred over alpha-aminoadipate.
3vpbB Argx from sulfolobus tokodaii complexed with lysw/glu/adp/mg/zn/sulfate (see paper)
29% identity, 72% coverage: 89:321/324 of query aligns to 67:277/282 of 3vpbB
- binding adenosine-5'-diphosphate: K87 (= K109), I125 (= I147), K127 (= K148), S132 (≠ Y153), G134 (= G155), V137 (≠ I158), Q167 (= Q195), Y169 (≠ F197), I170 (= I198), D176 (= D204), R200 (≠ V228), A201 (≠ N229), N202 (= N230), N249 (≠ L293), E250 (= E294)
- binding glutamic acid: Y190 (≠ I218), R192 (= R220), N202 (= N230), V203 (≠ L231), A204 (≠ S232), E256 (≠ S300), G259 (= G303)
- binding magnesium ion: D237 (= D265), E250 (= E294)
- binding zinc ion: E250 (= E294), N252 (= N296)
Q970U6 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
29% identity, 72% coverage: 89:321/324 of query aligns to 67:277/282 of Q970U6
- D237 (= D265) binding Mg(2+)
- E250 (= E294) binding Mg(2+); binding Mg(2+)
- N252 (= N296) binding Mg(2+)
4iwxA Rimk structure at 2.85a (see paper)
28% identity, 98% coverage: 3:321/324 of query aligns to 4:289/294 of 4iwxA
- binding adenosine-5'-diphosphate: V141 (= V146), K143 (= K148), E180 (= E196), Y181 (≠ F197), I182 (= I198), K183 (vs. gap), D189 (= D204), R213 (≠ V228), S214 (≠ N229), N215 (= N230), M261 (≠ L293), E262 (= E294)
- binding glutamic acid: R98 (≠ N105), R104 (≠ H111), Q107 (≠ Y114), L108 (= L115), R111 (= R118)
5zctH The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
28% identity, 98% coverage: 3:321/324 of query aligns to 2:287/292 of 5zctH
- binding phosphoaminophosphonic acid-adenylate ester: V139 (= V146), K141 (= K148), Q177 (= Q195), Y179 (≠ F197), I180 (= I198), D187 (= D204), F210 (≠ W227), R211 (≠ V228), S212 (≠ N229), N213 (= N230), R216 (= R233), L250 (≠ I267), M259 (≠ L293), E260 (= E294)
- binding magnesium ion: D248 (= D265), E260 (= E294)
5zctA The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
28% identity, 98% coverage: 3:321/324 of query aligns to 2:287/292 of 5zctA
- binding phosphoaminophosphonic acid-adenylate ester: K100 (= K109), K141 (= K148), T146 (≠ Y153), Q147 (≠ K154), G148 (= G155), Q177 (= Q195), Y179 (≠ F197), I180 (= I198), R211 (≠ V228), S212 (≠ N229), N213 (= N230), L250 (≠ I267), M259 (≠ L293)
7drnA Structure of atp-grasp ligase psnb complexed with precursor peptide psna2 and amppnp (see paper)
23% identity, 92% coverage: 6:304/324 of query aligns to 7:290/311 of 7drnA
- binding phosphoaminophosphonic acid-adenylate ester: K170 (= K148), T178 (≠ K156), Q202 (≠ E179), E203 (= E180), L205 (= L182), D209 (= D204), K269 (≠ I267), L279 (= L293), E280 (= E294)
- binding : R70 (= R69), Y79 (≠ F76), Y169 (≠ I147), P171 (= P149), G175 (≠ Y153), A176 (≠ K154), R179 (≠ D157), R190 (= R167), R211 (= R206), S284 (≠ T298), M286 (≠ S300)
7drpA Structure of atp-grasp ligase psnb complexed with phosphomimetic variant of minimal precursor, mg, and adp (see paper)
23% identity, 92% coverage: 6:304/324 of query aligns to 7:290/312 of 7drpA
- binding adenosine-5'-diphosphate: K170 (= K148), T178 (≠ K156), Q202 (≠ E179), E203 (= E180), L205 (= L182), D209 (= D204), K269 (≠ I267), L279 (= L293), E280 (= E294)
- binding magnesium ion: D267 (= D265), E280 (= E294), E280 (= E294), N282 (= N296)
- binding : R70 (= R69), G77 (= G74), Y79 (≠ F76), Y169 (≠ I147), G175 (≠ Y153), A176 (≠ K154), R179 (≠ D157), R190 (= R167), R193 (≠ D170), L194 (≠ R171), V199 (≠ G176), R211 (= R206), N282 (= N296), S284 (≠ T298), A285 (≠ P299), M286 (≠ S300)
P04425 Glutathione synthetase; GSH synthetase; GSH-S; GSHase; Glutathione synthase; EC 6.3.2.3 from Escherichia coli (strain K12) (see 3 papers)
26% identity, 65% coverage: 57:267/324 of query aligns to 74:275/316 of P04425
- C122 (≠ A106) mutation to A: No loss of activity.
- C195 (≠ I194) mutation to A: No loss of activity.
- C222 (≠ A217) mutation to A: No loss of activity.
- P227 (≠ A222) mutation to V: Loss of activity.
- R233 (≠ V228) mutation R->A,K: Loss of activity.
- G240 (= G235) mutation to V: Loss of activity.
- R241 (≠ S236) mutation R->A,K: No loss of activity.
Sites not aligning to the query:
- 289 C→A: No loss of activity.
B7UHZ4 Glutathione synthetase; GSH synthetase; GSH-S; GSHase; Glutathione synthase; EC 6.3.2.3 from Escherichia coli O127:H6 (strain E2348/69 / EPEC) (see paper)
26% identity, 65% coverage: 57:267/324 of query aligns to 74:275/316 of B7UHZ4
- R256 (≠ E251) modified: carbohydrate, N-beta-linked (GlcNAc) arginine
1gsaA Structure of glutathione synthetase complexed with adp and glutathione (see paper)
26% identity, 65% coverage: 57:267/324 of query aligns to 74:275/314 of 1gsaA
- active site: K125 (= K109), K160 (= K148), R210 (= R206), R225 (= R220), R233 (≠ V228), D273 (= D265)
- binding adenosine-5'-diphosphate: K125 (= K109), I158 (≠ V146), K160 (= K148), M165 (≠ Y153), G166 (≠ K154), G167 (= G155), I170 (= I158), Q198 (≠ F197), Y200 (≠ E199), L201 (≠ N200), D208 (= D204), R233 (≠ V228), G234 (≠ N229), N235 (= N230)
- binding glutathione: D88 (≠ V71), P89 (≠ G72), M165 (≠ Y153), R210 (= R206), R225 (= R220), L236 (= L231), A237 (≠ S232)
- binding magnesium ion: D273 (= D265)
- binding sulfate ion: M165 (≠ Y153), G166 (≠ K154), R210 (= R206), R225 (= R220), N235 (= N230), D273 (= D265)
Sites not aligning to the query:
A0A482PU20 Glutathione synthetase; GSH synthetase; GSH-S; GSHase; Glutathione synthase; EC 6.3.2.3 from Citrobacter rodentium (see paper)
26% identity, 65% coverage: 57:267/324 of query aligns to 74:275/315 of A0A482PU20
- R256 (≠ E251) modified: carbohydrate, N-beta-linked (GlcNAc) arginine; mutation to A: Abolished glycosylation by NleB.
Query Sequence
>WP_011023202.1 NCBI__GCF_000007345.1:WP_011023202.1
MKKIGIAITDPEDWTARALITAAKEKGFFPFILDLRIAEVSISSKTSEPATLFKAGEILL
SDLDALIVRDVGAGAFEGVSFRFDILRELEAGGVTVMNSPEAIQNAANKYHASYLLARAG
LPVPETVAVQSVEAALRAASGFGDAVIKPVFGYKGKDIARVKDGEIRFSDRKTGPGTVEE
ILEKLLEERGMLYIQEFIENPGRDIRAFVVGGKAIGAIYRKAAAGSWVNNLSRGGSADRC
VLAEEQKEIAEKAALAVGTTFAGIDIIEGAKAQTGNENKKTEDKSTGQGSRILEVNGTPS
GKGIFDAWGINPAEYILEYLQNIL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory