SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011024391.1 NCBI__GCF_000007345.1:WP_011024391.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2v5hB Controlling the storage of nitrogen as arginine: the complex of pii and acetylglutamate kinase from synechococcus elongatus pcc 7942 (see paper)
48% identity, 97% coverage: 5:293/299 of query aligns to 6:283/289 of 2v5hB

query
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2v5hB

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active site: K28 (= K27), G31 (= G30), G64 (= G63), D182 (= D192), K241 (= K251)
binding n-acetyl-l-glutamate: G62 (= G61), R85 (= R84), N178 (= N188), A181 (= A191)

2btyA Acetylglutamate kinase from thermotoga maritima complexed with its inhibitor arginine (see paper)
46% identity, 97% coverage: 1:291/299 of query aligns to 1:277/282 of 2btyA

query
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2btyA

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active site: K27 (= K27), G30 (= G30), G63 (= G63), D182 (= D192), K237 (= K251)
binding arginine: Y15 (= Y15), F19 (= F19), K196 (= K206), S214 (= S228), E266 (= E280), I267 (≠ L281), R270 (≠ D284), K271 (≠ G285), G274 (= G288), M276 (= M290)
binding n-acetyl-l-glutamate: G29 (= G29), G30 (= G30), G61 (= G61), G62 (= G62), N180 (= N190), D182 (= D192)

Q9X2A4 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
46% identity, 97% coverage: 1:291/299 of query aligns to 1:277/282 of Q9X2A4

query
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Q9X2A4

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N178 (= N188) binding substrate
K196 (= K206) binding L-arginine
S214 (= S228) binding L-arginine
EIFS 266:269 (≠ ELFT 280:283) binding L-arginine

Q9SCL7 Acetylglutamate kinase, chloroplastic; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AtNAGK; EC 2.7.2.8 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
44% identity, 97% coverage: 5:293/299 of query aligns to 69:347/347 of Q9SCL7

query
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Q9SCL7

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GA 94:95 (≠ GN 30:31) binding ATP
G126 (= G62) binding N-acetyl-L-glutamate
R148 (= R84) binding N-acetyl-L-glutamate
NINA 242:245 (= NINA 188:191) binding N-acetyl-L-glutamate
K260 (= K206) binding L-arginine
TD 265:266 (= TD 211:212) binding ATP
L271 (= L217) binding ATP
K282 (≠ S228) binding L-arginine
297:305 (vs. 243:251, 67% identical) binding ATP
EIMS 334:337 (≠ ELFT 280:283) binding L-arginine
G342 (= G288) binding L-arginine

Sites not aligning to the query:

1:50 modified: transit peptide, Chloroplast

2bufC Arginine feed-back inhibitable acetylglutamate kinase (see paper)
44% identity, 95% coverage: 8:291/299 of query aligns to 13:294/298 of 2bufC

query
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2bufC

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S

S

T

E

Q

E

I

L

M

K

E

A

D

G

I

F

I

A

K

R

D

D

I

V

V

V

L

L

L

M

R

K

Y

A

V

V

G

G

I

I

K

N

P

P

V

V

I

V

V

V

H

H

G

G

G
|
G

P

P

E

Q

I

I

T

G

E

D

K

L

M

L

E

K

R

R

M

L

G

S

K

I

K

E

A

S

E

H

F

F

F

I

Q

D

G

G

L

M

R

R

I

V

T

T

D

D

D

A

E

A

T

T

M

M

E

D

I

V

A

V

R

E

M

M

V

V

L

L

V

G

G

G

N

Q

I

V

N

N

T

K

K

D

I

I

V

V

S

N

L

L

I

I

G

N

I

R

F

H

G

G

K

S

G

A

V

I

G

G

F

L

T

T

G

G

Y

K

D

D

G

A

R

E

M

L

I

I

L

R

G

A

H

K

K

K

Q

L

A

T

V

V

K

T

R

R

V

Q

L

T

V

P

D

E

G

M

V

T

E

K

T

P

E

E

-

I

V

I

D

D

I

I

G

G

W

H

V

V

G

G

E

E

S

V

E

T

V

G

I

V

N

N

P

V

E

G

I

L

L

L

H

N

I

M

M

L

L

V

E

K

K

G

S

D

Y

F

I

I

P

P

V

V

I

I

S

A

P

P

I

I

A

G

V

V

D

G

A

S

K

N

G

G

N

E

A

S

L

Y

N

N

I

I

N

N

A

A

D
|
D

T

L

V

V

A

A

G

G

D

K

I

V

A

A

A

E

A

A

L

L

K

K

A

A

K

E

K

K

L

L

I

M

L

L

M

L

T

T

D
x
N

V

I

S

A

G

G

L
|
L

L
x
M

R

-

N

-

I

-

K

-

D

D

P

K

S

Q

S

G

R

Q

I

V

-

L

S

T

R

G

V

L

N

S

L

T

D

E

Q

Q

I

V

D

N

S

E

L

L

I

I

E

A

E

D

G

G

I
x
T

I
|
I

S
x
Y

G

G

G
|
G

M
|
M

I

L

P

P

K
|
K

I

I

K

R

G

C

A

A

A

L

V

E

A

A

V

V

K

Q

S

G

G

G

V

V

E

T

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

P

H

N

S

A

M

V

L

L

E

E

L

I

F

F

T

T

D

D

G

S

G

G

V

V

G

G

T

T

M

L

L

I

active site: K32 (= K27), G35 (= G30), G68 (= G63), D198 (= D192), K254 (= K251)
binding adenosine-5'-diphosphate: N36 (= N31), N218 (≠ D212), L222 (= L216), M223 (≠ L217), T246 (≠ I243), I247 (= I244), Y248 (≠ S245), G250 (= G247), M251 (= M248)

Q9HTN2 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
43% identity, 97% coverage: 1:291/299 of query aligns to 1:295/301 of Q9HTN2

query
sites
Q9HTN2

M
|
M

E

T

L

L

K

S

R

R

E

D

N

D

-

A

-

Q

-

V

-

A

-

K

V

V

L

L

I

S

E

E

A

A

L

L

P

P

Y

Y

M

I

Q

R

E

R

F

F

Y

V

D

G

S

K

I

T

M

L

V

V

I

I

K

K

V

Y

G

G

N

N

A

A

M

M

V

E

S

S

T

E

Q

E

I

L

M

K

E

A

D

G

I

F

I

A

K

R

D

D

I

V

V

V

L

L

L

M

R

K

Y

A

V

V

G

G

I

I

K

N

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

G

E

D

K

L

M

L

E

K

R

R

M

L

G

S

K

I

K

E

A

S

E

H

F

F

F

I

Q

D

G

G

L

M

R
|
R

I

V

T

T

D

D

D

A

E

A

T

T

M

M

E

D

I

V

A

V

R

E

M

M

V

V

L

L

V

G

G

G

N

Q

I

V

N

N

T

K

K

D

I

I

V

V

S

N

L

L

I

I

G

N

I

R

F

H

G

G

K

S

G

A

V

I

G

G

F

L

T

T

G

G

Y

K

D

D

G

A

R

E

M

L

I

I

L

R

G

A

H

K

K

K

Q

L

A

T

V

V

K

T

R

R

V

Q

L

T

V

P

D

E

G

M

V

T

E

K

T

P

E

E

-

I

V

I

D

D

I

I

G

G

W

H

V

V

G

G

E

E

S

V

E

T

V

G

I

V

N

N

P

V

E

G

I

L

L

L

H

N

I

M

M

L

L

V

E

K

K

G

S

D

Y

F

I

I

P

P

V

V

I

I

S

A

P

P

I

I

A

G

V

V

D

G

A

S

K

N

G

G

N

E

A

S

L

Y

N
|
N

I

I

N

N

A

A

D

D

T

L

V

V

A

A

G

G

D

K

I

V

A

A

A

E

A

A

L

L

K

K

A

A

K

E

K

K

L

L

I

M

L

L

M

L

T

T

D

N

V

I

S

A

G

G

L

L

L

M

R

-

N

-

I

-

K

-

D

D

P

K

S

Q

S

G

R

Q

I

V

-

L

S

T

R

G

V

L

N

S

L

T

D

E

Q

Q

I

V

D

N

S

E

L

L

I

I

E

A

E

D

G

G

I

T

I

I

S

Y

G

G

M

M

I

L

P

P

K

K

I

I

K

R

G

C

A

A

A

L

V

E

A

A

V

V

K

Q

S

G

G

G

V

V

E

T

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

P

H

N

S

A

M

V

L

L

E

E

L

I

F

F

T

T

D

D

G

S

G

G

V

V

G

G

T

T

M

L

L

I

M1 (= M1) modified: Initiator methionine, Removed
R90 (= R84) binding substrate
N195 (= N188) binding substrate

4usjB N-acetylglutamate kinase from arabidopsis thaliana in complex with pii from chlamydomonas reinhardtii (see paper)
44% identity, 97% coverage: 5:293/299 of query aligns to 3:281/281 of 4usjB

query
sites
4usjB

R

R

E

V

N

E

V

I

L

L

I

S

E

E

A

S

L

L

P

P

Y

F

M

I

Q

Q

E

K

F
|
F

Y

R

D

G

S

K

I

T

M

I

V

V

I

V

K
|
K

V

Y

G

G

G
|
G

N

A

A

A

M

M

V

T

S

S

T

P

Q

E

I

L

M

K

E

S

D

S

I

V

K

S

D

D

I

L

V

V

L

L

R

A

Y

C

V

V

G

G

I

L

K

R

P

P

V

I

I

L

V

V

H

H

G
|
G

P

P

E

D

I

I

T

N

E

R

K

Y

M

L

E

K

R

Q

M

L

G

N

K

I

K

P

A

A

E

E

F

F

F

R

Q

D

G

G

L

L

R
|
R

I

V

T

T

D

D

D

A

E

T

T

T

M

M

E

E

I

I

A

V

R

S

M

M

V

V

L

L

V

V

G

G

N

K

I

V

N

N

T

K

K

N

I

L

V

V

S

S

L

L

I

I

G

N

I

A

F

A

G

G

G

A

K

T

G

A

V

V

G

G

F

L

T

S

G

G

Y

H

D

D

G

G

R

R

M

L

I

L

L

T

G

A

H

R

K

P

Q

-

A

-

V

-

K

-

R

-

V

-

L

-

V

-

D

-

G

-

V

V

E

P

T

N

E

S

V

A

D

Q

I

L

G

G

W

F

V

V

G

G

E

E

S

V

E

A

V

R

I

V

N

D

P

P

E

S

I

V

L

L

H

R

I

P

M

L

L

V

E

D

K

Y

S

G

Y

Y

I

I

P

P

V

V

I

I

S

A

P

S

I

V

A

A

V

A

D

D

A

D

K

S

G

G

N

Q

A

A

L

Y

N
|
N

I

I

N

N

A
|
A

D
|
D

T

T

V

V

A

A

G

G

D

E

I

L

A

A

L

L

K

G

A

A

K

E

K
|
K

L

L

I

I

L

L

M

L

T

T

D

D

V

V

S

A

G

G

L

I

L

L

R

E

N

N

I

K

K

E

D

D

P

P

S

S

R

L

I

I

S
x
K

R

E

V

I

N

D

L

I

D

K

Q

G

I

V

D

K

S

K

L

M

I

I

E

E

E

D

G

G

I

K

I

V

S

A

G

G

M

M

I

I

P

P

K
|
K

I

V

K

K

G

C

A

C

A

I

V

R

A

S

V

L

K

A

S

Q

G

G

V

V

E

K

R

T

A

A

H

S

V

I

I

I

N

D

G

G

S

R

V

R

S

Q

H

H

S

S

M

L

L

L

L

H

E
|
E

L
x
I

F

M

T
x
S

D

D

G
x
E

G
|
G

V

A

G

G

T

T

M
|
M

L

I

Y

T

G

G

active site: K25 (= K27), G28 (= G30), G61 (= G63), D180 (= D192), K239 (= K251)
binding arginine: F17 (= F19), K194 (= K206), K216 (≠ S228), E268 (= E280), I269 (≠ L281), S271 (≠ T283), E273 (≠ G285), G274 (= G286), M278 (= M290)
binding n-acetyl-l-glutamyl 5-phosphate: G59 (= G61), G60 (= G62), G61 (= G63), R82 (= R84), N176 (= N188), A179 (= A191)

2rd5B Structural basis for the regulation of n-acetylglutamate kinase by pii in arabidopsis thaliana (see paper)
44% identity, 97% coverage: 5:293/299 of query aligns to 5:283/283 of 2rd5B

query
sites
2rd5B

R

R

E

V

N

E

V

I

L

L

I

S

E

E

A

S

L

L

P

P

Y

F

M

I

Q

Q

E

K

F

F

Y

R

D

G

S

K

I

T

M

I

V

V

I

V

K
|
K

V

Y

G

G

G
|
G

N
x
A

A

A

M

M

V

T

S

S

T

P

Q

E

I

L

M

K

E

S

D

S

I

V

K

S

D

D

I

L

V

V

L

L

R

A

Y

C

V

V

G

G

I

L

K

R

P

P

V

I

I

L

V

V

H

H

G
|
G

G

G

G
|
G

P

P

E

D

I

I

T

N

E

R

K

Y

M

L

E

K

R

Q

M

L

G

N

K

I

K

P

A

A

E

E

F

F

F

R

Q

D

G

G

L

L

R
|
R

I

V

T

T

D

D

D

A

E

T

T

T

M

M

E

E

I

I

A

V

R

S

M

M

V

V

L

L

V

V

G

G

N

K

I

V

N

N

T

K

K

N

I

L

V

V

S

S

L

L

I

I

G

N

I

A

F

A

G

G

G

A

K

T

G

A

V

V

G

G

F

L

T

S

G

G

Y

H

D

D

G

G

R

R

M

L

I

L

L

T

G

A

H

R

K

P

Q

-

A

-

V

-

K

-

R

-

V

-

L

-

V

-

D

-

G

-

V

V

E

P

T

N

E

S

V

A

D

Q

I

L

G

G

W

F

V

V

G

G

E

E

S

V

E

A

V

R

I

V

N

D

P

P

E

S

I

V

L

L

H

R

I

P

M

L

L

V

E

D

K

Y

S

G

Y

Y

I

I

P

P

V

V

I

I

S

A

P

S

I

V

A

A

V

A

D

D

A

D

K

S

G

G

N

Q

A

A

L

Y

N
|
N

I

I

N
|
N

A
|
A

D
|
D

T

T

V

V

A

A

G

G

D

E

I

L

A

A

L

L

K

G

A

A

K

E

K
|
K

L

L

I

I

L

L

M

L

T
|
T

D
|
D

V

V

S

A

G

G

L

I

L
|
L

R

E

N

N

I
x
K

K

E

D

D

P

P

S

S

R

L

I

I

S
x
K

R

E

V

I

N

D

L

I

D

K

Q

G

I

V

D

K

S

K

L

M

I

I

E

E

E

D

G

G

I

K

I

V

S
x
A

G

G

G
|
G

M
|
M

I

I

P

P

K
|
K

I

V

K

K

G

C

A

C

A

I

V

R

A

S

V

L

K

A

S

Q

G

G

V

V

E

K

R

T

A

A

H

S

V

I

I

I

N

D

G

G

S

R

V

R

S

Q

H

H

S

S

M

L

L

L

L

H

E
|
E

L

I

F

M

T
x
S

D

D

G
x
E

G

G

V

A

G
|
G

T

T

M
|
M

L

I

Y

T

G

G

active site: K27 (= K27), G30 (= G30), G63 (= G63), D182 (= D192), K241 (= K251)
binding adenosine-5'-diphosphate: A31 (≠ N31), T201 (= T211), D202 (= D212), L207 (= L217), K210 (≠ I220), A235 (≠ S245), G237 (= G247), M238 (= M248), K241 (= K251)
binding arginine: K196 (= K206), K218 (≠ S228), E270 (= E280), S273 (≠ T283), E275 (≠ G285), G278 (= G288), M280 (= M290)
binding n-acetyl-l-glutamate: G61 (= G61), R84 (= R84), N178 (= N188), N180 (= N190), A181 (= A191)

2bufA Arginine feed-back inhibitable acetylglutamate kinase (see paper)
44% identity, 95% coverage: 8:291/299 of query aligns to 13:286/292 of 2bufA

query
sites
2bufA

V

V

L

L

I

S

E

E

A

A

L

L

P

P

Y

Y

M

I

Q

R

E

R

F

F

Y

V

D

G

S

K

I

T

M

L

V

V

I

I

K
|
K

V

Y

G

G

G
|
G

N

N

A

A

M

M

V

E

S

S

T

E

Q

E

I

L

M

K

E

A

D

G

I

F

I

A

K

R

D

D

I

V

V

V

L

L

L

M

R

K

Y

A

V

V

G

G

I

I

K

N

P

P

V

V

I

V

V

V

H

H

G
|
G

P

P

E

Q

I

I

T

G

E

D

K

L

M

L

E

K

R

R

M

L

G

S

K

I

K

E

A

S

E

H

F

F

F

I

Q

D

G

G

L

M

R
|
R

I

V

T

T

D

D

D

A

E

A

T

T

M

M

E

D

I

V

A

V

R

E

M

M

V

V

L

L

V

G

G

G

N

Q

I

V

N

N

T

K

K

D

I

I

V

V

S

N

L

L

I

I

G

N

I

R

F

H

G

G

K

S

G

A

V

I

G

G

F

L

T

T

G

G

Y

K

D

D

G

A

R

E

M

L

I

I

L

R

G

A

H

K

K

K

Q

L

A

T

V

V

K

T

R

R

V

Q

L

I

V

-

D

-

G

-

V

-

E

-

T

-

E

-

V

I

D

D

I

I

G

G

W

H

V

V

G

G

E

E

S

V

E

T

V

G

I

V

N

N

P

V

E

G

I

L

L

L

H

N

I

M

M

L

L

V

E

K

K

G

S

D

Y

F

I

I

P

P

V

V

I

I

S

A

P

P

I

I

A

G

V

V

D

G

A

S

K

N

G

G

N

E

A

S

L

Y

N
|
N

I

I

N

N

A
|
A

D
|
D

T

L

V

V

A

A

G

G

D

K

I

V

A

A

A

E

A

A

L

L

K

K

A

A

K

E

K

K

L

L

I

M

L

L

M

L

T

T

D

N

V

I

S

A

G

G

L

L

L

M

R

-

N

-

I

-

K

-

D

D

P

K

S

Q

S

G

R

Q

I

V

-

L

S

T

R

G

V

L

N

S

L

T

D

E

Q

Q

I

V

D

N

S

E

L

L

I

I

E

A

E

D

G

G

I

T

I

I

S

Y

G

G

M

M

I

L

P

P

K
|
K

I

I

K

R

G

C

A

A

A

L

V

E

A

A

V

V

K

Q

S

G

G

G

V

V

E

T

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

P

H

N

S

A

M

V

L

L

E

E

L

I

F

F

T

T

D

D

G

S

G

G

V

V

G

G

T

T

M

L

L

I

active site: K32 (= K27), G35 (= G30), G68 (= G63), D190 (= D192), K246 (= K251)
binding n-acetyl-l-glutamate: G66 (= G61), G67 (= G62), R89 (= R84), N186 (= N188), A189 (= A191)

2bufK Arginine feed-back inhibitable acetylglutamate kinase (see paper)
42% identity, 95% coverage: 8:291/299 of query aligns to 13:271/273 of 2bufK

query
sites
2bufK

V

V

L

L

I

S

E

E

A

A

L

L

P

P

Y

Y

M

I

Q

R

E

R

F

F

Y

V

D

G

S

K

I

T

M

L

V

V

I

I

K
|
K

V

Y

G

G

G
|
G

N

N

A

A

M

M

V

E

S

S

T

E

Q

E

I

L

M

K

E

A

D

G

I

F

I

A

K

R

D

D

I

V

V

V

L

L

L

M

R

K

Y

A

V

V

G

G

I

I

K

N

P

P

V

V

I

V

V

V

H

H

G

G

G
|
G

P

P

E

Q

I

I

T

G

E

D

K

L

M

L

E

K

R

R

M

L

G

S

K

I

K

E

A

S

E

H

F

-

Q

-

G

-

L

-

R
|
R

I

V

T

T

D

D

D

A

E

A

T

T

M

M

E

D

I

V

A

V

R

E

M

M

V

V

L

L

V

G

G

G

N

Q

I

V

N

N

T

K

K

D

I

I

V

V

S

N

L

L

I

I

G

N

I

R

F

H

G

G

K

S

G

A

V

I

G

G

F

L

T

T

G

G

Y

K

D

D

G

A

R

E

M

L

I

I

L

R

G

A

H

K

K

K

Q

L

A

T

V

-

K

-

R

-

V

-

L

-

V

-

D

-

G

-

V

-

E

-

T

-

E

-

V

-

D

-

I

-

G

-

W

-

V

V

G

G

E

E

S

V

E

T

V

G

I

V

N

N

P

V

E

G

I

L

L

L

H

N

I

M

M

L

L

V

E

K

K

G

S

D

Y

F

I

I

P

P

V

V

I

I

S

A

P

P

I

I

A

G

V

V

D

G

A

S

K

N

G

G

N

E

A

S

L

Y

N
|
N

I

I

N
|
N

A
|
A

D
|
D

T

L

V

V

A

A

G

G

D

K

I

V

A

A

A

E

A

A

L

L

K

K

A

A

K

E

K

K

L

L

I

M

L

L

M

L

T

T

D

N

V

I

S

A

G

G

L

L

L

M

R

-

N

-

I

-

K

-

D

D

P

K

S

Q

S

G

R

Q

I

V

-

L

S

T

R

G

V

L

N

S

L

T

D

E

Q

Q

I

V

D

N

S

E

L

L

I

I

E

A

E

D

G

G

I

T

I

I

S

Y

G

G

M

M

I

L

P

P

K
|
K

I

I

K

R

G

C

A

A

A

L

V

E

A

A

V

V

K

Q

S

G

G

G

V

V

E

T

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

P

H

N

S

A

M

V

L

L

E

E

L

I

F

F

T

T

D

D

G

S

G

G

V

V

G

G

T

T

M

L

L

I

active site: K32 (= K27), G35 (= G30), G68 (= G63), D175 (= D192), K231 (= K251)
binding n-acetyl-l-glutamate: R84 (= R84), N171 (= N188), N173 (= N190), A174 (= A191)

7nlyA Crystal structure of mycobacterium tuberculosis argb in complex with 2-chlorobenzimidazole.
41% identity, 95% coverage: 5:289/299 of query aligns to 11:288/293 of 7nlyA

query
sites
7nlyA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 2-chloranyl-1~{H}-benzimidazole: V124 (= V118), L167 (≠ M166)

7nloA Crystal structure of mycobacterium tuberculosis argb in complex with l-arginine
41% identity, 95% coverage: 5:289/299 of query aligns to 7:284/289 of 7nloA

query
sites
7nloA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y
x
W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V

V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M

L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K
|
K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S
|
S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H
|
H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E
|
E

L
|
L

F

F

T
|
T

D

D

G
x
A

G

G

V

T

G
|
G

T
|
T

binding arginine: W17 (≠ Y15), K203 (= K206), S225 (= S228), H262 (= H267), E275 (= E280), L276 (= L281), T278 (= T283), A280 (≠ G285), G283 (= G288), T284 (= T289)

Sites not aligning to the query:

binding arginine: 285

7nlpA Crystal structure of mycobacterium tuberculosis argb in complex with l-canavanine
41% identity, 95% coverage: 5:289/299 of query aligns to 10:287/292 of 7nlpA

query
sites
7nlpA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y
x
W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V

V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M

L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K
|
K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S
|
S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H
|
H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E
|
E

L
|
L

F

F

T
|
T

D
|
D

G
x
A

G

G

V

T

G
|
G

T
|
T

binding l-canavanine: W20 (≠ Y15), K206 (= K206), S228 (= S228), H265 (= H267), E278 (= E280), L279 (= L281), T281 (= T283), D282 (= D284), A283 (≠ G285), G286 (= G288), T287 (= T289)

Sites not aligning to the query:

binding l-canavanine: 288

7nlxA Crystal structure of mycobacterium tuberculosis argb in complex with 7-(trifluoromethyl)quinolin-4-ol.
41% identity, 95% coverage: 5:289/299 of query aligns to 8:285/290 of 7nlxA

query
sites
7nlxA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M
x
L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 7-(trifluoromethyl)quinolin-4-ol: V121 (= V118), L130 (≠ M127), L164 (≠ M166)

7nlnA Crystal structure of mycobacterium tuberculosis argb in complex with n-acetyl-glutamate
41% identity, 95% coverage: 5:289/299 of query aligns to 8:285/290 of 7nlnA

query
sites
7nlnA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G
|
G

P

P

E

Q

I
|
I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R
|
R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V

V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M

L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N
|
N

I

I

N

N

A
|
A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding n-acetyl-l-glutamate: G64 (= G61), G65 (= G62), G66 (= G63), I69 (= I66), R87 (= R84), N186 (= N188), A189 (= A191)

7nnbA Crystal structure of mycobacterium tuberculosis argb in complex with 2,8-bis(trifluoromethyl)quinolin-4-ol.
41% identity, 95% coverage: 5:289/299 of query aligns to 9:286/291 of 7nnbA

query
sites
7nnbA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 2,8-bis(trifluoromethyl)quinolin-4-ol: V122 (= V118), L165 (≠ M166)

7nn8A Crystal structure of mycobacterium tuberculosis argb in complex with 1h-indole-3-carbonitrile.
41% identity, 95% coverage: 5:289/299 of query aligns to 9:286/291 of 7nn8A

query
sites
7nn8A

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 1~{H}-indole-3-carbonitrile: V122 (= V118), L165 (≠ M166)

7nm0A Crystal structure of mycobacterium tuberculosis argb in complex with 1-(2,6-dihydroxyphenyl)ethan-1-one.
41% identity, 95% coverage: 5:289/299 of query aligns to 9:286/291 of 7nm0A

query
sites
7nm0A

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 1-[2,6-bis(oxidanyl)phenyl]ethanone: V122 (= V118), L165 (≠ M166)

7nlzA Crystal structure of mycobacterium tuberculosis argb in complex with 5-methoxy-6-(trifluoromethyl)indole.
41% identity, 95% coverage: 5:289/299 of query aligns to 9:286/291 of 7nlzA

query
sites
7nlzA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F

I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y

R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 5-methoxy-6-(trifluoromethyl)-1~{H}-indole: V122 (= V118), L165 (≠ M166)

7nlwA Crystal structure of mycobacterium tuberculosis argb in complex with 2-(5-methoxy-1h-indol-3-yl)acetonitrile
41% identity, 95% coverage: 5:289/299 of query aligns to 9:286/290 of 7nlwA

query
sites
7nlwA

R

K

E

A

N

Q

V

V

L

L

I

A

E

E

A

A

L

L

P

P

Y

W

M

L

Q

K

E

Q

F

L

Y

H

D

G

S

K

I

V

M

V

V

V

I

V

K

K

V

Y

G

G

N

N

A

A

M

M

V

T

S

D

T

D

Q

T

I

L

M

R

E

R

D

A

I

F

I

A

K

A

D

D

I

M

V

A

L

F

L

L

R

R

Y

N

V

C

G

G

I

I

K

H

P

P

V

V

I

V

V

V

H

H

G

G

P

P

E

Q

I

I

T

T

E

A

K

M

M

L

E

R

R

R

M

L

G

G

K

I

K

E

A

G

E

D

F

F

F

K

Q

G

G

G

L

F

R

R

I

V

T

T

D

T

D

P

E

E

T

V

M

L

E

D

I

V

A

A

R

R

M

M

V

V

L

L

V

F

G

G

N

Q

I

V

N

G

T

R

K

E

I

L

V

V

S

N

L

L

I

I

G

N

I

A

F

H

G

G

G

P

K

Y

G

A

V
|
V

G

G

F
x
I

T

T

G

G

Y

E

D

D

G

A

R

Q

M

L

I

F

L

-

G

-

H

-

K

-

Q

T

A

A

V

V

K

R

R

R

-

S

V

V

L

T

V

V

D

D

G

G

V

V

E

A

T

T

E

-

V

-

D

D

I

I

G

G

W

L

V

V

G

G

E

D

S

V

E

D

V

Q

I

V

N

N

P

T

E

A

I

A

L

M

H

L

I

D

M
x
L

L

V

E

A

K

A

S

G

Y
x
R

I

I

P

P

V

V

I

V

S

S

P

T

I

L

A

A

V

P

D

D

A

A

K

D

G

G

N

V

A

V

L

H

N

N

I

I

N

N

A

A

D

D

T

T

V

A

A

A

G

A

D

A

I

V

A

A

A

E

A

A

L

L

K

G

A

A

K

E

K

K

L

L

I

L

L

M

M

L

T

T

D

D

V

I

S

D

G

G

L

L

L

Y

R

T

N

R

I

W

K

P

D

D

P

R

S

D

S

S

R

L

I

V

S

S

R

E

V

I

N

D

L

T

D

G

Q

T

I

L

D

A

S

Q

L

L

I

L

E

P

E

T

G

-

I

-

I

L

S

E

G

S

G

G

M

M

I

V

P

P

K

K

I

V

K

E

G

A

A

C

A

L

V

R

A

A

V

V

K

I

S

G

G

G

V

V

E

P

R

S

A

A

H

H

V

I

I

I

N

D

G

G

S

R

V

V

S

T

H

H

S

C

M

V

L

L

L

V

E

E

L

L

F

F

T

T

D

D

G

A

G

G

V

T

G

G

T

T

binding 2-(5-methoxy-1~{H}-indol-3-yl)ethanenitrile: V122 (= V118), I124 (≠ F120), L165 (≠ M166), R170 (≠ Y171)

Query Sequence

>WP_011024391.1 NCBI__GCF_000007345.1:WP_011024391.1
MELKRENVLIEALPYMQEFYDSIMVIKVGGNAMVSTQIMEDIIKDIVLLRYVGIKPVIVH
GGGPEITEKMERMGKKAEFFQGLRITDDETMEIARMVLVGNINTKIVSLIGIFGGKGVGF
TGYDGRMILGHKQAVKRVLVDGVETEVDIGWVGESEVINPEILHIMLEKSYIPVISPIAV
DAKGNALNINADTVAGDIAAALKAKKLILMTDVSGLLRNIKDPSSRISRVNLDQIDSLIE
EGIISGGMIPKIKGAAVAVKSGVERAHVINGSVSHSMLLELFTDGGVGTMLYGPDHPPV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory