SitesBLAST

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
28% identity, 81% coverage: 10:152/176 of query aligns to 5:151/173 of P10880

query
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P10880

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C13 (≠ A18) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K20) mutation to M: Loss of activity. Increased thermostability.
D34 (= D39) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
29% identity, 84% coverage: 9:156/176 of query aligns to 2:147/160 of 3mufA

query
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3mufA

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binding adenosine-5'-diphosphate: G10 (= G17), S11 (≠ A18), G12 (= G19), K13 (= K20), S14 (= S21), S15 (≠ T22), M98 (≠ A108), R106 (= R116), G147 (= G156)
binding shikimate-3-phosphate: M9 (≠ A16), D32 (= D39), R56 (≠ L63), G78 (= G84), G79 (= G85), G80 (≠ S86), R115 (≠ I125), R131 (= R140)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 148

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
29% identity, 87% coverage: 7:159/176 of query aligns to 1:151/168 of 3n2eA

query
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3n2eA

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binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ M15), R45 (≠ Q51), F48 (≠ I54), R57 (≠ L63), G80 (= G85), G81 (≠ S86), M84 (≠ Y89), R116 (≠ V126), R132 (= R140), L135 (= L143), Y136 (= Y144)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
27% identity, 81% coverage: 10:152/176 of query aligns to 5:136/158 of 1shkA

query
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1shkA

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binding magnesium ion: R92 (≠ K98), A93 (≠ N99), G95 (≠ S101), Q129 (≠ N145), A132 (= A148)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
28% identity, 81% coverage: 10:152/176 of query aligns to 5:141/162 of 2shkB

query
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2shkB

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binding adenosine-5'-diphosphate: G12 (= G17), C13 (≠ A18), G14 (= G19), K15 (= K20), T16 (≠ S21), T17 (= T22), R110 (= R116)
binding magnesium ion: Q134 (≠ N145), A137 (= A148)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 145, 147

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
26% identity, 81% coverage: 10:152/176 of query aligns to 5:151/170 of 1e6cA

query
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1e6cA

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binding phosphate ion: R11 (≠ A16), G12 (= G17), M15 (≠ K20), T16 (≠ S21)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
26% identity, 84% coverage: 6:153/176 of query aligns to 7:156/179 of 4y0aA

query
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4y0aA

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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ A16), D40 (= D39), F63 (≠ L62), R64 (≠ L63), G86 (= G85), G87 (≠ S86), L126 (≠ I125), R143 (= R140)
binding sulfate ion: G18 (= G17), A19 (= A18), G20 (= G19), K21 (= K20), T22 (≠ S21), E112 (≠ R111), R124 (= R123), R137 (≠ K134)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/165 of 3bafA

query
sites
3bafA

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K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R
|
R

G

P

I

L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ A16), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116), R116 (= R123)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D39), R135 (= R140)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/165 of 2dfnA

query
sites
2dfnA

L

L

I

V

G

G

M

L

A
x
P

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D
|
D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L
x
I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L
x
F

L
x
R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G
|
G

S
x
G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R
|
R

G

P

I

L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding adenosine-5'-diphosphate: G11 (= G17), S12 (≠ A18), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116), R116 (= R123)
binding chloride ion: P10 (≠ A16), K14 (= K20), G79 (= G85)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D39), I44 (≠ L50), F56 (≠ L62), R57 (≠ L63), G78 (= G84), G79 (= G85), G80 (≠ S86), R135 (= R140)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/165 of 1we2A

query
sites
1we2A

L

L

I

V

G

G

M

L

A
x
P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D
|
D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L
x
I

Q

A

V

D

L

I

I
x
F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L

R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G
|
G

S
x
G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R
|
R

G

P

I
x
L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding adenosine-5'-diphosphate: P10 (≠ A16), G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116), R116 (= R123)
binding 3-dehydroshikimate: D33 (= D39), I44 (≠ L50), F48 (≠ I54), G78 (= G84), G79 (= G85), G80 (≠ S86), L118 (≠ I125), R135 (= R140)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/168 of 1zyuA

query
sites
1zyuA

L

L

I

V

G

G

M

L

A
x
P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D
|
D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L

I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L
x
R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G

G

S
x
G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R

R

G
x
P

I
x
L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ A16), G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D39), R57 (≠ L63), G80 (≠ S86), P117 (≠ G124), L118 (≠ I125), R135 (= R140)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 149, 152, 154

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/169 of 4bqsA

query
sites
4bqsA

L

L

I

V

G

G

M

L

A
x
P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D
|
D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L

I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L

R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G

G

S

G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R

R

G

P

I

L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding adenosine-5'-diphosphate: P10 (≠ A16), G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D39), R135 (= R140)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/169 of 2iyyA

query
sites
2iyyA

L

L

I

V

G

G

M

L

A

P

G

G

A

S

G

G

K
|
K

S

S

T

T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D
|
D

R

V

L

A

I

I

T
x
E

D

Q

R

R

T

T

G

G

M

R

P
x
S

L
x
I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L
x
R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G

G

G
|
G

S
x
G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

-

S

H

K

T

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

I

T

P

G

N

G

A

N

R

T

R

V

R

R

G

P

I
x
L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R
|
R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding magnesium ion: E37 (≠ T43), S43 (≠ P49), I44 (≠ L50)
binding phosphate ion: K14 (= K20), G79 (= G85)
binding shikimate-3-phosphate: K14 (= K20), D33 (= D39), R57 (≠ L63), G79 (= G85), G80 (≠ S86), L118 (≠ I125), R135 (= R140)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/179 of 2iyvA

query
sites
2iyvA

L

L

I

V

G

G

M

L

A

P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D

D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L

I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L

R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G

G

S

G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

H

S

T

K

-

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R

R

G

P

I

L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R

R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding adenosine-5'-diphosphate: G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:147/178 of 2iywA

query
sites
2iywA

L

L

I

V

G

G

M

L

A

P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

I

L

D

D

V

T

D

D

R

V

L

A

I

I

T

E

D

Q

R

R

T

T

G

G

M

R

P

S

L

I

Q

A

V

D

L

I

I

F

D

A

K

T

Q

D

G

G

D

E

S

Q

A

E

L

F

L

R

R

R

F

I

E

E

E

D

A

V

I

V

L

R

G

A

L

A

G

L

Q

A

V

D

D

H

N

D

C

G

I

V

I

L

S

S

P

L

G

G

S

G

V

A

I

V

Y

T

S

S

E

P

K

G

S

V

M

R

V

A

Y

A

L

L

K

A

N

G

I

H

S

T

K

-

V

V

F

Y

L

L

D

E

A

I

S

S

F

A

R

A

S

E

I

G

A

V

R

R

R
|
R

I

T

P

G

N

G

A

N

R

T

R

V

R

R

G

P

I

L

V

L

G

A

L

G

R

P

D

D

R

R

S

A

L

E

K

K

-

Y

-

R

E

A

L

L

F

M

E

A

E

K

R

R

L

A

V

P

L

L

Y

Y

N

R

K

R

Y

V

A

A

D

T

F

M

S

R

V

V

binding adenosine-5'-triphosphate: G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
31% identity, 80% coverage: 12:152/176 of query aligns to 6:145/163 of 1u8aA

query
sites
1u8aA

L

L

I

V

G

G

M

L

A

P

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

A

R

L

L

A

A

K

K

R

A

L

L

D

G

Y

V

T

G

F

L

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L

D

D

V

T

D
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D

R

V

L

A

I

I

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D

Q

R

R

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T

G

G

M

R

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I

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I
x
F

D

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K

T

Q

D

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G

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x
R

R

R

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E

E

D

A

V

I

V

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R

G

A

L

A

G

L

Q

A

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D

D

H

N

D

C

G

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P

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G

G

G
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G

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x
G

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T

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S

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P

K

G

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M

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V

A

Y

A

L

L

K

A

N

G

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-

S

H

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R

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G

N

G

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R

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-

G

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x
L

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K

K

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R

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Y

Y

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V

binding adenosine-5'-diphosphate: G11 (= G17), G13 (= G19), K14 (= K20), S15 (= S21), T16 (= T22), R109 (= R116)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D39), F48 (≠ I54), R57 (≠ L63), G79 (= G85), G80 (≠ S86), L116 (≠ I125), R133 (= R140)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
29% identity, 52% coverage: 9:99/176 of query aligns to 5:97/682 of 7tbvA

query
sites
7tbvA

N

S

I

I

T

I

L

V

I

I

G

G

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M

A

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G

G

A

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G

G

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L

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K

K

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K

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R

-

F

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E

Q

E

E

A

A

I

I

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G

A

L

-

G

-

Q

-

V

-

D

K

N

E

C

C

-

F

-

T

-

K

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F

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S

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K

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G

-

Y

I

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E

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G

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E

K

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Q

L

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K

N

S

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
39% identity, 32% coverage: 2:58/176 of query aligns to 858:914/1583 of P07547

query
sites
P07547

G

G

R

P

K

D

R

R

V

A

V

N

M

A

N

S

I

I

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G

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M

A

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G

G

A

A

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G

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A

G

G

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N

A

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G

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

Query Sequence

>WP_011032038.1 NCBI__GCF_000007065.1:WP_011032038.1
MGRKRVVMNITLIGMAGAGKSTIGKALAKRLDYTFIDVDRLITDRTGMPLQVLIDKQGDS
ALLRFEEEAILGLGQVDNCIISPGGSVIYSEKSMVYLKNISKVVFLDASFRSIARRIPNA
RRRGIVGLRDRSLKELFEERLVLYNKYADFSVKIRGKENIQGIVDRVIRLCFMVES

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory