SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011034136.1 NCBI__GCF_000007065.1:WP_011034136.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
41% identity, 94% coverage: 15:292/295 of query aligns to 3:284/284 of Q58761

query
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Q58761

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DNE 34:36 (≠ DGE 46:48) binding
D212 (= D220) binding
STGGT 216:220 (≠ ATGGT 224:228) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
39% identity, 94% coverage: 15:292/295 of query aligns to 3:274/274 of 1u9zA

query
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1u9zA

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binding adenosine monophosphate: F32 (= F44), D34 (= D46), E36 (= E48), R92 (= R101), Q93 (= Q102), F97 (= F106), H125 (= H132)
binding ribose-5-phosphate: D163 (= D171), D202 (= D220), I205 (= I223), S206 (≠ A224), T207 (= T225), G208 (= G226), T210 (= T228)

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
32% identity, 92% coverage: 13:284/295 of query aligns to 3:275/287 of 3mbiA

query
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3mbiA

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binding adenosine-5'-diphosphate: F34 (= F44), D36 (= D46), E38 (= E48), R93 (= R101), Q94 (= Q102), Y98 (≠ F106), H126 (= H132), K186 (= K194), R188 (= R196)
binding magnesium ion: H95 (≠ D103), H126 (= H132)
binding phosphate ion: R188 (= R196), S216 (≠ A224), T217 (= T225), G218 (= G226), T220 (= T228)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
32% identity, 92% coverage: 13:284/295 of query aligns to 1:273/285 of 3mbiD

query
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3mbiD

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binding adenosine-5'-diphosphate: F32 (= F44), D34 (= D46), E36 (= E48), R91 (= R101), Q92 (= Q102), Y96 (≠ F106), H124 (= H132), K184 (= K194), R186 (= R196)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R101), H124 (= H132), K184 (= K194), R186 (= R196), D210 (= D220), D211 (= D221), I212 (≠ M222), S214 (≠ A224), T215 (= T225), G216 (= G226), T218 (= T228)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
32% identity, 92% coverage: 13:284/295 of query aligns to 1:273/286 of Q97CA5

query
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Q97CA5

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E

H

K

N

V

I

T

F

L

I

I

I

Q

G

S

N

T

T

P

H

T

S

D

D

S

A

D

E

F

V

V

M

A

E

L

M

L

I

Q

L

L

T

I

L

D

S

A

A

C

I

E

Q

G

D

-

Y

-

R

A

T

A

K

E

S

I

V

N

N

V

I

I

A

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D
x
H

K

Q

K

R

F

Y

K

K

S

N

G

G

E

E

P

P

V

I

S

S

A

S

R

Q

A

I

I

L

A

T

R

E

C

I

I

Y

N

S

A

S

-

Y

-

S

D

N

R

S

V

I

F

A

T

T

I

V

N

D

I

I

H

H

E

D

K

E

S

K

V

T

L

L

E

S

H

Y

F

S

P

K

C

V

P

K

A

F

E

S

N

D

L

L

D

H

A

A

A

N

A

D

L

A

I

I

G

V

S

R

Y

Y

V

Y

A

K

G

N

F

V

G

D

L

V

E

D

R

-

P

-

M

Y

L

V

V

V

A

S

P

P

D

D

E

D

G

G

A

G

R

L

G

A

L

R

V

V

K

A

N

D

V

I

A

S

S

A

G

K

H

L

G

G

F

K

D

K

H

H

D

F

H

F

L

I

Q

E

K

K

T

K

R
|
R

L

I

S

D

G

D

D

R

T

T

V

V

V

E

I

M

K

K

T

V

K

P

N

N

L

V

D

D

V

V

T

N

G

G

R

K

H

K

V

L

L

I

V

V

D
|
D

D

D

M

I

I

I

A
x
S

T
|
T

G
|
G

T
|
T

M

I

A

A

E

K

S

S

I

S

R

G

M

L

L

L

K

R

S

E

Q

K

G

G

A

A

I

S

D

K

V

I

H

Y

L

V

A

S

C

A

V

V

H

H

P

G

V

L

L

F

T

V

R

N

N

G

A

S

A

E

L

N

R

K

L

I

F

L

H

Q

A

-

G

N

V

A

K

D

D

E

I

I

I

H

G

V

T

T

D

D

T

T

L

V

E

E

K

S

A

K

E

F

S

S

K

D

L

I

S

S

V

V

DGE 34:36 (= DGE 46:48) binding
RQH 91:93 (≠ RQD 101:103) binding
R186 (= R196) binding
D210 (= D220) binding
STGGT 214:218 (≠ ATGGT 224:228) binding

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
32% identity, 92% coverage: 13:284/295 of query aligns to 1:273/284 of 3lpnA

query
sites
3lpnA

L

M

K

K

I

I

G

A

G

L

P
x
R

A

S

S

S

Q

L

L
x
K

L

L

A

A

S

A

R
|
R

T

I

A

A

R

E

A

E

L

L

G

K

T

T

E

E

P

P

V

V

L

M

C

P

E

D

F

E

N

R

R

R

F
|
F

P

P

D
|
D

G

G

E
|
E

L

L

Y

Y

L

L

R

R

I

Y

A

D

E

E

E

D

I

L

E

T

N

G

E

H

K

N

V

I

T

F

L

I

I

I

Q

G

S

N

T

T

P

H

T
x
S

D

D

S

A

D

E

F

V

V

M

A

E

L

M

L

I

Q

L

L

T

I

L

D

S

A

A

C

I

E

Q

G

D

-

Y

-

R

A

T

A

K

E

S

I

V

N

N

V

I

I

A

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D
x
H

K

Q

K

R

F
x
Y

K

K

S

N

G

G

E

E

P

P

V

I

S

S

A

S

R

Q

A

I

I

L

A

T

R

E

C

I

I

Y

N

S

A

S

-

Y

-

S

D

N

R

S

V

I

F

A

T

T

I

V

N

D

I

I

H
|
H

E
x
D

K

E

S

K

V

T

L

L

E

S

H

Y

F

S

P

K

C

V

P

K

A

F

E

S

N

D

L

L

D

H

A

A

A

N

A

D

L

A

I

I

G

V

S

R

Y

Y

V

Y

A

K

G

N

F

V

G

D

L

V

E

D

R

-

P

-

M

Y

L

V

V

V

A

S

P

P

D

D

E

D

G

G

A

G

R

L

G

A

L

R

V

V

K

A

N

D

V

I

A

S

S

A

G

K

H

L

G

G

F

K

D

K

H

H

D

F

H

F

L

I

Q

E

K

K

T

K

R

R

L

I

S

D

G

D

D

R

T

T

V

V

V

E

I

M

K

K

T

V

K

P

N

N

L

V

D

D

V

V

T

N

G

G

R

K

H

K

V

L

L

I

V

V

D

D

M

I

I

I

A
x
S

T
|
T

G
|
G

G

G

T
|
T

M

I

A

A

E

K

S

S

I

S

R

G

M

L

L

L

K

R

S

E

Q

K

G

G

A

A

I

S

D

K

V

I

H

Y

L

V

A

S

C

A

V

V

H

H

P

G

V

L

L

F

T

V

R

N

N

G

A

S

A

E

L

N

R

K

L

I

F

L

H

Q

A

-

G

N

V

A

K

D

D

E

I

I

I

H

G

V

T

T

D

D

T

T

L

V

E

E

K

S

A

K

E

F

S

S

K

D

L

I

S

S

V

V

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F44), D34 (= D46), E36 (= E48), R91 (= R101), Q92 (= Q102), H93 (≠ D103), Y96 (≠ F106), H124 (= H132), D125 (≠ E133)
binding sulfate ion: R7 (≠ P19), K11 (≠ L23), R15 (= R27), S58 (≠ T70), S214 (≠ A224), T215 (= T225), G216 (= G226), T218 (= T228)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
32% identity, 95% coverage: 13:292/295 of query aligns to 10:303/317 of P14193

query
sites
P14193

L

L

K

K

I

I

I

F

G

S

G

L

P

N

A

S

S

N

Q

P

L

E

L

L

A

A

S

K

R

E

T

I

A

A

R

D

A

I

L

V

G

G

T

V

E

Q

P

L

V

G

L

K

C

C

E

S

F

V

N

T

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

Q

L

I

R

N

I

I

A

E

E

E

E

S

I

I

E

R

N

G

E

C

K

D

V

C

T

Y

L

I

I

I

Q

Q

S

S

T

T

-

S

-

D

P

P

T

V

D

N

S

E

D

H

F

I

V

M

A

E

L

L

Q

I

L

M

I

V

D

D

A

A

C

L

E

K

-

R

-

A

G

S

A

A

A

K

E

T

I

I

N

N

V

I

V

V

I

I

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

K

R

K

K

F

A

K

R

S

S

G

R

E

E

P

P

V

I

S

T

A

A

R

K

A

L

I

F

A

A

R

N

C

L

I

L

N

E

-

T

-

A

-

G

A

A

D

T

R

R

V

V

F

I

T

A

I

L

N

D

I

L

H

H

E

A

K

P

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

I

P

P

A

I

E

D

N

H

L

L

D

M

A

G

A

V

A

P

L

I

I

L

G

G

S

E

Y

Y

V

F

A

E

G

G

F

K

G

N

L

L

E

E

R

D

P

I

M

V

L

I

V

V

A

S

P

P

D

D

E

H

G

G

A

G

R

V

G

T

L

R

V

A

K

R

N

K

V

L

A

A

S

D

G

R

H

L

G

K

F

A

D

P

H

I

D

A

H

I

L

I

Q

D

K
|
K

T

R

R
|
R

L

P

S
x
R

G

P

D
x
N

T

V

V

A

V
x
E

I

V

K

M

T

N

K

I

N

V

L

G

D

N

V

I

T

E

G

G

R

K

H

T

V

A

V

I

L

L

V

I

D

D

M

I

I

I

A
x
D

T
|
T

G
x
A

G
|
G

T
|
T

M

I

A

T

E

L

S

A

I

A

R

N

M

A

L

L

K

V

S

E

Q

N

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

C

C

C

V

T

H

H

P

P

V

V

L

L

T

S

R

G

N

P

A

A

A

V

L

E

R

R

L

I

F

N

H

N

A

S

G

T

V

I

K

K

D

E

I

L

I

V

G

V

T

T

D

N

T

S

L

I

E

K

K

L

A

P

E

E

S

E

K

K

-

K

-

I

-

E

-

R

-

F

-

K

-

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

A

A

L

I

RQ 102:103 (= RQ 101:102) binding
K198 (= K194) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R196) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ S198) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ D200) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ V203) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ ATGGT 224:228) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
33% identity, 95% coverage: 13:292/295 of query aligns to 2:282/295 of 1dkuA

query
sites
1dkuA

L

L

K

K

I

I

I

F

G

S

G

L

P

N

A

S

S

N

Q

P

L

E

L

L

A

A

S

K

R

E

T

I

A

A

R

D

A

I

L

V

G

G

T

V

E

Q

P

L

V

G

L

K

C

C

E

S

F

V

N

T

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

Q

L

I

R

N

I

I

A

E

E

E

E

S

I

I

E

R

N

G

E

C

K

D

V

C

T

Y

L

I

I

I

Q

Q

S

S

T

T

-

S

-

D

P

P

T

V

D

N

S

E

D

H

F

I

V

M

A

E

L

L

Q

I

L

M

I

V

D

D

A

A

C

L

E

K

-

R

-

A

G

S

A

A

A

K

E

T

I

I

N

N

V

I

V

V

I

I

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

K

R

K
|
K

F

A

K

R

S
|
S

G
x
R

E

E

P

P

V

I

S

T

A

A

R

K

A

L

I

F

A

A

R

N

C

L

I

L

N

E

-

T

-

A

-

G

A

A

D

T

R

R

V

V

F

I

T

A

I

L

N

D

I

L

H
|
H

E

A

K

P

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

I

P

P

A

I

E
x
D

N

H

L

L

D

M

A

G

A

V

A

P

L

I

I

L

G

G

S

E

Y

Y

V

F

A

E

G

G

F

K

G

N

L

L

E

E

R

D

P

I

M

V

L

I

V

V

A

S

P

P

D

D

E

H

G

G

A

G

R

V

G

T

L

R

V

A

K

R

N

K

V

L

A

A

S

D

G

-

H

-

G

-

F

-

D

-

H

-

D

-

H

-

L

-

Q

-

K

-

T

-

R

R

L

L

S

K

G

A

D

P

T

I

V

A

V

I

I

I

-

D

K

K

T

R

K

M

N

N

L

I

-

V

-

G

D

N

V

I

T

E

G

G

R

K

H

T

V

A

V

I

L

L

V

I

D

D

M

I

I

I

A

D

T

T

G

A

G

G

T

T

M

I

A

T

E

L

S

A

I

A

R

N

M

A

L

L

K

V

S

E

Q

N

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

C

C

C

V

T

H

H

P

P

V

V

L

L

T

S

R

G

N

P

A

A

A

V

L

E

R

R

L

I

F

N

H

N

A

S

G

T

V

I

K

K

D

E

I

L

I

V

G

V

T

T

D

N

T

S

L

I

E

K

-

L

K

K

A

I

E

E

-

R

-

F

S

K

K

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

A

A

L

I

binding methyl phosphonic acid adenosine ester: R94 (= R101), H128 (= H132)
binding phosphomethylphosphonic acid adenosyl ester: K98 (= K105), S101 (= S108), R102 (≠ G109), D141 (≠ E145)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 290, 291, 292, 295

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
33% identity, 95% coverage: 13:292/295 of query aligns to 2:284/297 of 1ibsA

query
sites
1ibsA

L

L

K

K

I

I

I

F

G

S

G

L

P

N

A

S

S

N

Q

P

L

E

L

L

A

A

S

K

R

E

T

I

A

A

R

D

A

I

L

V

G

G

T

V

E

Q

P

L

V

G

L

K

C

C

E

S

F

V

N

T

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

Q

L

I

R

N

I

I

A

E

E

E

E

S

I

I

E

R

N

G

E

C

K

D

V

C

T

Y

L

I

I

I

Q

Q

S

S

T

T

-

S

-

D

P

P

T

V

D

N

S

E

D

H

F

I

V

M

A

E

L

L

Q

I

L

M

I

V

D

D

A

A

C

L

E

K

-

R

-

A

G

S

A

A

A

K

E

T

I

I

N

N

V

I

V

V

I

I

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

K
x
R

K

K

F

-

K

-

S

S

G

R

E

E

P

P

V

I

S

T

A

A

R

K

A

L

I

F

A

A

R

N

C

L

I

L

N

E

-

T

-

A

-

G

A

A

D

T

R

R

V

V

F

I

T

A

I

L

N

D

I

L

H
|
H

E

A

K

P

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

I

P

P

A

I

E

D

N

H

L

L

D

M

A

G

A

V

A

P

L

I

I

L

G

G

S

E

Y

Y

V

F

A

E

G

G

F

K

G

N

L

L

E

E

R

D

P

I

M

V

L

I

V

V

A

S

P

P

D

D

E

H

G

G

A

G

R

V

G

T

L

R

V

A

K

R

N

K

V

L

A

A

S

D

G

-

H

-

G

-

F

-

D

-

H

-

D

-

H

-

L

-

Q

-

K

-

T

-

R

R

L

L

S

K

G

A

D

P

T

I

V

A

V

I

I

I

-

D

K

K

T

R

K

M

N

N

L

I

-

V

-

G

D

N

V

I

T

E

G

G

R

K

H

T

V

A

V

I

L

L

V

I

D

D

M

I

I

I

A
x
D

T
|
T

G
x
A

G

G

T
|
T

M

I

A

T

E

L

S

A

I

A

R

N

M

A

L

L

K

V

S

E

Q

N

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

C

C

C

V

T

H

H

P

P

V

V

L

L

T

S

R

G

N

P

A

A

A

V

L

E

R

R

L

I

F

N

H

N

A

S

G

T

V

I

K

K

D

E

I

L

I

V

G

V

T

T

D

N

T

S

L

I

E

K

K

L

A

P

E

E

S

E

K

K

-

K

-

I

-

E

-

R

-

F

-

K

-

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

A

A

L

I

binding methyl phosphonic acid adenosine ester: R94 (= R101), Q95 (= Q102), R97 (≠ K104), H126 (= H132)
binding sulfate ion: D209 (≠ A224), T210 (= T225), A211 (≠ G226), T213 (= T228)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
33% identity, 95% coverage: 13:292/295 of query aligns to 4:286/299 of 1ibsB

query
sites
1ibsB

L

L

K

K

I

I

I

F

G

S

G

L

P

N

A

S

S

N

Q

P

L

E

L

L

A

A

S

K

R

E

T

I

A

A

R

D

A

I

L

V

G

G

T

V

E

Q

P

L

V

G

L

K

C

C

E

S

F

V

N

T

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

Q

L

I

R

N

I

I

A

E

E

E

E

S

I

I

E

R

N

G

E

C

K

D

V

C

T

Y

L

I

I

I

Q

Q

S

S

T

T

-

S

-

D

P

P

T

V

D

N

S

E

D

H

F

I

V

M

A

E

L

L

Q

I

L

M

I

V

D

D

A

A

C

L

E

K

-

R

-

A

G

S

A

A

A

K

E

T

I

I

N

N

V

I

V

V

I

I

P

P

Y

Y

M

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

K
x
R

K

K

F

-

K

-

S

S

G

R

E

E

P

P

V

I

S

T

A

A

R

K

A

L

I

F

A

A

R

N

C

L

I

L

N

E

-

T

-

A

-

G

A

A

D

T

R

R

V

V

F

I

T

A

I

L

N

D

I

L

H

H

E

A

K

P

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

I

P

P

A

I

E

D

N

H

L

L

D

M

A

G

A

V

A

P

L

I

I

L

G

G

S

E

Y

Y

V

F

A

E

G

G

F

K

G

N

L

L

E

E

R

D

P

I

M

V

L

I

V

V

A

S

P

P

D

D

E

H

G

G

A

G

R

V

G

T

L

R

V

A

K

R

N

K

V

L

A

A

S

D

G

-

H

-

G

-

F

-

D

-

H

-

D

-

H

-

L

-

Q

-

K

-

T

-

R

R

L

L

S

K

G

A

D

P

T

I

V

A

V

I

I

I

-

D

K

K

T

R

K

M

N

N

L

I

-

V

-

G

D

N

V

I

T

E

G

G

R

K

H

T

V

A

V

I

L

L

V

I

D

D

M

I

I

I

A

D

T

T

G

A

G

G

T

T

M

I

A

T

E

L

S

A

I

A

R

N

M

A

L

L

K

V

S

E

Q

N

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

C

C

C

V

T

H

H

P

P

V

V

L

L

T

S

R

G

N

P

A

A

A

V

L

E

R

R

L

I

F

N

H

N

A

S

G

T

V

I

K

K

D

E

I

L

I

V

G

V

T

T

D

N

T

S

L

I

E

K

K

L

A

P

E

E

S

E

K

K

-

K

-

I

-

E

-

R

-

F

-

K

-

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

A

A

L

I

binding methyl phosphonic acid adenosine ester: R96 (= R101), Q97 (= Q102), R99 (≠ K104)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
31% identity, 88% coverage: 15:275/295 of query aligns to 3:271/291 of Q97Z86

query
sites
Q97Z86

I

I

G

G

P

S

A

A

S

T

Q

N

L

G

L

I

A

D

S

E

R

S

T

L

A

S

R

K

A

I

L

L

G

S

T

I

E

P

P

L

V

V

L

K

C

V

E

E

F

N

N

K

R

I

F

F

P

P

D

D

G

G

E

E

L

S

Y

Y

L

I

R

R

I

V

A

P

E

S

I

I

E

R

N

D

E

E

K

E

V

V

T

L

L

L

I

V

Q

Q

S

T

T

T

-

D

-

Y

P

P

T

Q

D

D

S

K

D

H

F

L

V

I

A

E

L

L

L

F

Q

L

L

I

I

A

D

E

A

T

C

I

E

R

-

D

-

L

G

G

A

A

A

K

E

K

I

L

N

T

V

A

V

I

I

V

P

P

Y

Y

M

L

G

A

Y

Y

A

S

R

R

Q

Q

D

D

K

R

F

F

K

K

S

D

G

G

E

E

P

A

V

I

S

S

A

I

R

K

A

T

I

I

A

L

R

H

C

I

I

L

N

S

A

E

D

V

R

G

V

V

F

N

T

T

I

L

N

V

I

V

-

V

-

E

-

P

H

H

E

K

K

P

S

E

V

E

L

L

E

S

H

Y

F

F

P

K

C

G

P

E

A

L

E

K

N

I

L

V

D

H

A

P

A

Y

A

H

L

Q

I

I

G

A

S

R

Y

K

V

I

A

K

G

E

F

I

G

-

L

I

E

E

R

D

P

P

M

F

L

I

V

L

A

A

P

P

D

D

E

R

G

G

A

A

R

L

G

D

L

R

V

A

K

R

N

K

V

I

A

A

S

E

G

E

H

I

G

N

F

A

D

P

H

Y

D

S

H

Y

L

I

Q

E

K

K

T

E

R

R

-

D

-

R

L

T

S

T

G

G

D

E

T

V

V

R

V

I

I

K

K

E

T

A

K

P

N

N

L

I

D

N

V

L

T

K

G

G

R

K

H

D

V

V

L

I

V

I

D

D

M

I

I

I

A
x
S

T
|
T

G
|
G

T
|
T

M

I

A

V

E

Q

S

A

I

T

R

R

M

L

L

A

K

Y

S

S

Q

L

G

G

A

A

I

K

D

S

V

V

H

T

L

A

A

A

C

A

V

I

H

H

P

L

V

L

L

L

T

V

R

G

N

G

A

A

A

K

L

E

R

R

L

L

F

R

H

E

A

V

G

G

V

V

K

K

D

T

I

L

I

I

G

G

T

T

D

N

T

T

L

I

STGGT 220:224 (≠ ATGGT 224:228) binding

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
32% identity, 96% coverage: 13:294/295 of query aligns to 3:293/308 of 4s2uA

query
sites
4s2uA

L

M

K

K

I

L

I

F

G

A

G

G

P

N

A

A

S

T

Q

P

L

E

L

L

A

A

S

Q

R

R

T

I

A

A

R

N

A

R

L

L

G

Y

T

T

E

S

P

L

V

G

L

D

C

A

E

A

F

V

N

G

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

S

L

V

R

Q

I

I

A

N

E

E

E

N

I

V

E

R

N

G

E

G

K

D

V

I

T

F

L

I

I

I

Q

Q

S

S

T

T

-

C

-

A

P

P

T

T

D

N

S

D

D

N

F

L

V

M

A

E

L

L

L

V

Q

V

L

M

I

V

D

D

A

A

C

L

E

R

-

R

-

A

G

S

A

A

A

G

E

R

I

I

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

K

R

F

V

K

R

S

S

G

A

E

R

-

V

P

P

V

I

S

T

A

A

R

K

A

V

I

V

A

A

-

D

-

F

-

L

R

S

C

S

I

V

N

G

A

V

D

D

R

R

V

V

F

L

T

T

I

V

N

D

I

L

H

H

E

A

K

E

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

V

P

P

A

V

E

D

N

N

L

V

D

F

A

G

A

S

A

P

L

I

I

L

G

L

S

E

Y

D

V

M

A

L

G

Q

F

L

G

N

L

L

E

D

R

N

P

P

M

I

L

V

V

V

A

S

P

P

D

D

E

I

G

G

-

G

-

V

-

R

A

A

R

R

G

A

L

I

V

A

K

K

N

L

V

L

A

-

S

-

G

-

H

N

G

D

F

T

D

D

H

M

D

A

H

I

L

I

Q

D

K

K

T

R

R

R

L

P

S

R

G

A

D

N

T

V

V

S

V

Q

I

V

K

M

T

H

K

I

N

I

L

G

D

D

V

V

T

A

G

G

R

R

H

D

V

C

V

V

L

L

V

V

D

D

M

M

I

I

A
x
D

T

T

G

G

T
|
T

M

L

A

C

E

K

S

A

I

A

R

E

M

A

L

L

K

K

S

E

Q

R

G

G

A

A

I

K

D

R

V

V

H

F

L

A

A

Y

C

A

V

T

H

H

P

P

V

I

L

F

T

S

R

G

N

N

A

A

L

N

R

N

L

L

F

R

H

N

A

S

G

V

V

I

K

D

D

E

I

V

G

V

T

C

D

D

T

T

L

I

E

P

K

L

A

S

E

D

S

E

K

I

L

K

S

S

V

L

A

P

P

N

L

V

I

R

A

T

E

L

A

T

L

L

S

S

G

G

binding magnesium ion: D223 (≠ A224), T227 (= T228)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
32% identity, 96% coverage: 13:294/295 of query aligns to 4:294/315 of P0A717

query
sites
P0A717

L

M

K

K

I

L

I

F

G

A

G

G

P

N

A

A

S

T

Q

P

L

E

L

L

A

A

S

Q

R

R

T

I

A

A

R

N

A

R

L

L

G

Y

T

T

E

S

P

L

V

G

L

D

C

A

E

A

F

V

N

G

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

S

L

V

R

Q

I

I

A

N

E

E

E

N

I

V

E

R

N

G

E

G

K

D

V

I

T

F

L

I

I

I

Q

Q

S

S

T

T

-

C

-

A

P

P

T

T

D

N

S

D

D

N

F

L

V

M

A

E

L

L

L

V

Q

V

L

M

I

V

D

D

A

A

C

L

E

R

-

R

-

A

G

S

A

A

A

G

E

R

I

I

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

K

R

F

V

K

R

S

S

G

A

E

R

-

V

P

P

V

I

S

T

A

A

R

K

A

V

I

V

A

A

-

D

-

F

-

L

R

S

C

S

I

V

N

G

A

V

D

D

R

R

V

V

F

L

T

T

I

V

N
x
D

I

L

H

H

E

A

K

E

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

V

P

P

A

V

E

D

N

N

L

V

D

F

A

G

A

S

A

P

L

I

I

L

G

L

S

E

Y

D

V

M

A

L

G

Q

F

L

G

N

L

L

E

D

R

N

P

P

M

I

L

V

V

V

A

S

P

P

D

D

E

I

G

G

-

G

-

V

-

R

A

A

R

R

G

A

L

I

V

A

K

K

N

L

V

L

A

-

S

-

G

-

H

N

G

D

F

T

D

D

H

M

D

A

H

I

L

I

Q

D

K

K

T

R

R

R

L

P

S

R

G

A

D

N

T

V

V

S

V

Q

I

V

K

M

T

H

K

I

N

I

L

G

D

D

V

V

T

A

G

G

R

R

H

D

V

C

V

V

L

L

V

V

D
|
D

M

M

I

I

A
x
D

T

T

G

G

T

T

M

L

A

C

E

K

S

A

I

A

R

E

M

A

L

L

K

K

S

E

Q

R

G

G

A

A

I

K

D

R

V

V

H

F

L

A

A

Y

C

A

V

T

H

H

P

P

V

I

L

F

T

S

R

G

N

N

A

A

L

N

R

N

L

L

F

R

H

N

A

S

G

V

V

I

K

D

D

E

I

V

G

V

T

C

D

D

T

T

L

I

E

P

K

L

A

S

E

D

S

E

K

I

L

K

S

S

V

L

A

P

P

N

L

V

I

R

A

T

E

L

A

T

L

L

S

S

G

G

D129 (≠ N130) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D220) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D221) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ A224) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
32% identity, 96% coverage: 13:294/295 of query aligns to 2:292/311 of 6asvC

query
sites
6asvC

L

M

K

K

I

L

I

F

G

A

G

G

P

N

A

A

S

T

Q

P

L

E

L

L

A

A

S

Q

R

R

T

I

A

A

R

N

A

R

L

L

G

Y

T

T

E

S

P

L

V

G

L

D

C

A

E

A

F

V

N

G

R

R

F

F

P

S

D

D

G

G

E

E

L

V

Y

S

L

V

R

Q

I

I

A

N

E

E

E

N

I

V

E

R

N

G

E

G

K

D

V

I

T

F

L

I

I

I

Q

Q

S

S

T

T

-

C

-

A

P

P

T

T

D

N

S

D

D

N

F

L

V

M

A

E

L

L

L

V

Q

V

L

M

I

V

D

D

A

A

C

L

E

R

-

R

-

A

G

S

A

A

A

G

E

R

I

I

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

K

R

F

V

K

R

S

S

G

A

E

R

-

V

P

P

V

I

S

T

A

A

R

K

A

V

I

V

A

A

-

D

-

F

-

L

R

S

C

S

I

V

N

G

A

V

D

D

R

R

V

V

F

L

T

T

I

V

N

D

I

L

H

H

E

A

K

E

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

V

P

P

A

V

E

D

N

N

L

V

D

F

A

G

A

S

A

P

L

I

I

L

G

L

S

E

Y

D

V

M

A

L

G

Q

F

L

G

N

L

L

E

D

R

N

P

P

M

I

L

V

V

V

A

S

P

P

D
|
D

E

I

G

G

-

G

-

V

-

R

A

A

R

R

G

A

L

I

V

A

K

K

N

L

V

L

A

-

S

-

G

-

H

N

G

D

F

T

D

D

H

M

D

A

H

I

L

I

Q

D

K

K

T

R

R

R

L

P

S

R

G

A

D

N

T

V

V

S

V

Q

I

V

K

M

T

H

K

I

N

I

L

G

D

D

V

V

T

A

G

G

R

R

H

D

V

C

V

V

L

L

V

V

D
|
D

M

M

I

I

A
x
D

T
|
T

G
|
G

G

G

T
|
T

M
x
L

A

C

E

K

S

A

I

A

R

E

M

A

L

L

K

K

S

E

Q

R

G

G

A

A

I

K

D

R

V

V

H

F

L

A

A

Y

C

A

V

T

H

H

P

P

V

I

L

F

T

S

R

G

N

N

A

A

L

N

R

N

L

L

F

R

H

N

A

S

G

V

V

I

K

D

D

E

I

V

G

V

T

C

D

D

T

T

L

I

E

P

K

L

A

S

E

D

S

E

K

I

L

K

S

S

V

L

A

P

P

N

L

V

I

R

A

T

E

L

A

T

L

L

S

S

G

G

binding phosphate ion: D168 (= D171), D218 (= D220), D219 (= D221), D222 (≠ A224), T223 (= T225), G224 (= G226), T226 (= T228), L227 (≠ M229)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
31% identity, 96% coverage: 13:294/295 of query aligns to 2:286/307 of 7xmvA

query
sites
7xmvA

L

M

K

K

I

L

I

F

G

A

G

G

P

N

A

A

S

T

Q

P

L

E

L

L

A

A

S

Q

R

R

T

I

A

A

R

N

A

R

L

L

G

Y

T

T

E

S

P

L

V

G

L

D

C

A

E

A

F

V

N

G

R

R

F
|
F

P

S

D
|
D

G

G

E
|
E

L

V

Y

S

L

V

R

Q

I

I

A

N

E

E

E

N

I

V

E

R

N

G

E

G

K

D

V

I

T

F

L

I

I

I

Q

Q

S

S

T

T

-

C

-

A

P

P

T

T

D

N

S

D

D

N

F

L

V

M

A

E

L

L

L

V

Q

V

L

M

I

V

D

D

A

A

C

L

E

R

-

R

-

A

G

S

A

A

A

G

E

R

I

I

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

K
x
R

K

R

F
x
V

K
x
R

S

S

G

A

E

R

-

V

P

P

V

I

S

T

A

A

R

K

A

V

I

V

A

A

-

D

-

F

-

L

R

S

C

S

I

V

N

G

A

V

D

D

R

R

V

V

F

L

T

T

I

V

N

D

I

L

H
|
H

E

A

K
x
E

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

V

P

P

A

V

E

D

N

N

L

V

D
x
F

A

G

A
x
S

A

P

L

I

I

L

G

L

S

E

Y

D

V

M

A

L

G

Q

F

L

G

N

L

L

E

D

R

N

P

P

M

I

L

V

V

V

A

S

P

P

D

D

E

I

G

G

A

-

R

-

G

G

L

V

V
|
V

K

R

N

A

V

R

A
|
A

S

-

G

-

H

-

G

-

F

-

D

-

H

-

D

-

H

-

L

I

Q

A

K

K

T

L

R

L

L

N

S

D

G

T

D

D

T

M

V

A

V

I

I

I

K

D

T

K

K

R

N

R

L

Q

-

V

-

M

-

H

-

I

-

G

D

D

V

V

T

A

G

G

R

R

H

D

V

C

V

V

L

L

V

V

D
|
D

M
|
M

I

I

A
x
D

T
|
T

G

G

G
|
G

T
|
T

M

L

A

C

E

K

S

A

I

A

R

E

M

A

L

L

K

K

S

E

Q

R

G

G

A

A

I

K

D

R

V

V

H

F

L

A

A

Y

C

A

V

T

H

H

P

P

V

I

L

F

T

S

R

G

N

N

A

A

L

N

R

N

L

L

F

R

H

N

A

S

G

V

V

I

K

D

D

E

I

V

G

V

T

C

D

D

T

T

L

I

E

P

K

L

A

S

E

D

S

E

K

I

L

K

S

S

V

L

A

P

P

N

L

V

I

R

A

T

E

L

A

T

L

L

S

S

G

G

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R101), R97 (≠ K104), H129 (= H132)
binding adenosine monophosphate: R97 (≠ K104), V99 (≠ F106), R100 (≠ K107), E131 (≠ K134), F145 (≠ D148), S147 (≠ A150), V173 (= V178), A177 (= A182)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D220), D213 (= D221), M214 (= M222), D216 (≠ A224), T217 (= T225), G219 (= G227), T220 (= T228)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
31% identity, 96% coverage: 13:294/295 of query aligns to 2:286/307 of 7xmuA

query
sites
7xmuA

L

M

K

K

I

L

I

F

G

A

G

G

P

N

A

A

S

T

Q

P

L

E

L

L

A

A

S

Q

R

R

T

I

A

A

R

N

A

R

L

L

G

Y

T

T

E

S

P

L

V

G

L

D

C

A

E

A

F

V

N

G

R

R

F
|
F

P

S

D
|
D

G

G

E
|
E

L

V

Y

S

L

V

R

Q

I

I

A

N

E

E

E

N

I

V

E

R

N

G

E

G

K

D

V

I

T

F

L

I

I

I

Q

Q

S

S

T

T

-

C

-

A

P

P

T

T

D

N

S

D

D

N

F

L

V

M

A

E

L

L

L

V

Q

V

L

M

I

V

D

D

A

A

C

L

E

R

-

R

-

A

G

S

A

A

A

G

E

R

I

I

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

K
x
R

K

R

F

V

K
x
R

S

S

G

A

E

R

-

V

P

P

V

I

S

T

A

A

R

K

A

V

I

V

A

A

-

D

-

F

-

L

R

S

C

S

I

V

N

G

A

V

D

D

R

R

V

V

F

L

T

T

I

V

N

D

I

L

H
|
H

E

A

K
x
E

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

V

P

P

A

V

E

D

N

N

L

V

D
x
F

A

G

A
x
S

A

P

L

I

I

L

G

L

S

E

Y

D

V

M

A

L

G

Q

F

L

G

N

L

L

E

D

R

N

P

P

M

I

L

V

V

V

A

S

P

P

D
|
D

E

I

G

G

A

-

R

-

G

G

L

V

V
|
V

K

R

N

A

V

R

A

A

S

-

G

-

H

-

G

-

F

-

D

-

H

-

D

-

H

-

L

I

Q

A

K

K

T

L

R

L

L

N

S

D

G

T

D

D

T

M

V

A

V

I

I

I

K

D

T

K

K

R

N

R

L

Q

-

V

-

M

-

H

-

I

-

G

D

D

V

V

T

A

G

G

R

R

H

D

V

C

V

V

L

L

V

V

D
|
D

D

D

M
|
M

I

I

A
x
D

T
|
T

G

G

T

T

M

L

A

C

E

K

S

A

I

A

R

E

M

A

L

L

K

K

S

E

Q

R

G

G

A

A

I

K

D

R

V

V

H

F

L

A

A

Y

C

A

V

T

H

H

P

P

V

I

L

F

T

S

R

G

N

N

A

A

L

N

R

N

L

L

F

R

H

N

A

S

G

V

V

I

K

D

D

E

I

V

G

V

T

C

D

D

T

T

L

I

E

P

K

L

A

S

E

D

S

E

K

I

L

K

S

S

V

L

A

P

P

N

L

V

I

R

A

T

E

L

A

T

L

L

S

S

G

G

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R101), Q95 (= Q102), R97 (≠ K104), R97 (≠ K104), R100 (≠ K107), H129 (= H132), E131 (≠ K134), F145 (≠ D148), S147 (≠ A150), V173 (= V178)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D171), D212 (= D220), M214 (= M222), D216 (≠ A224), T217 (= T225)

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
31% identity, 88% coverage: 15:275/295 of query aligns to 3:258/278 of 4twbA

query
sites
4twbA

I

I

G

G

P

S

A

A

S

T

Q

N

L

G

L

I

A

D

S

E

R

S

T

L

A

S

R

K

A

I

L

L

G

S

T

I

E

P

P

L

V

V

L

K

C

V

E

E

F

N

N

K

R

I

F
|
F

P

P

D
|
D

G

G

E
|
E

L

S

Y

Y

L

I

R

R

I

V

A

P

E

S

I

I

E

R

N

D

E

E

K

E

V

V

T

L

L

L

I

V

Q

Q

S

T

T

T

-

D

-

Y

P

P

T

Q

D

D

S

K

D

H

F

L

V

I

A

E

L

L

L

F

Q

L

L

I

I

A

D

E

A

T

C

I

E

R

-

D

-

L

G

G

A

A

A

K

E

K

I

L

N

T

V

A

V

I

I

V

P

P

Y

Y

M

L

G

A

Y

Y

A

S

R
|
R

Q

Q

D

D

K

R

F
|
F

K

K

S

D

G

G

E

E

P

A

V

I

S

S

A

I

R

K

A

T

I

I

A

L

R

H

C

I

I

L

N

S

A

E

D

V

R

G

V

V

F

N

T

T

I

L

N

V

I

V

-

V

-

E

-

P

H
|
H

E

K

K

P

S

E

V

E

L

L

E

S

H

Y

F

F

P

K

C

G

P

E

A

L

E

K

N

I

L

V

D

H

A

P

A

Y

A

H

L

Q

I

I

G

A

S

R

Y

K

V

I

A

K

G

E

F

I

G

-

L

I

E

E

R

D

P

P

M

F

L

I

V

L

A

A

P

P

D

D

E

R

G

G

A

A

R

L

G

D

L

R

V

A

K

R

N

K

V

I

A

A

S

E

G

E

H

I

G

N

F

A

D

P

H

Y

D

S

H

Y

L

I

Q

E

K

K

T

E

R

R

L

-

S

-

G

-

D

-

T

-

V

-

I

-

K

-

T

-

K

-

N

N

L

I

D

N

V

L

T

K

G

G

R

K

H

D

V

V

L

I

V

I

D

D

M

I

I

I

A

S

T

T

G

G

T

T

M

I

A

V

E

Q

S

A

I

T

R

R

M

L

L

A

K

Y

S

S

Q

L

G

G

A

A

I

K

D

S

V

V

H

T

L

A

A

A

C

A

V

I

H

H

P

L

V

L

L

L

T

V

R

G

N

G

A

A

A

K

L

E

R

R

L

L

F

R

H

E

A

V

G

G

V

V

K

K

D

T

I

L

I

I

G

G

T

T

D

N

T

T

L

I

binding adenosine monophosphate: F32 (= F44), D34 (= D46), E36 (= E48), R93 (= R101), F98 (= F106), H127 (= H132)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
34% identity, 95% coverage: 13:292/295 of query aligns to 2:293/307 of 5t3oA

query
sites
5t3oA

L

L

K

L

I

I

I

F

G

S

G

G

P

Q

A

S

S

N

Q

R

L

P

L

L

A

A

S

Q

R

A

T

I

A

A

R

E

A

A

L

L

G

G

T

L

E

P

P

L

V

G

L

K

C

S

E

T

F

T

N

L

R

R

F

F

P

A

D

N

G

D

E

N

L

L

Y

F

L

V

R

R

I

Y

A

E

E

E

E
x
S

I

L

E
x
R

N

E

E

G

K

D

V

V

T

F

L

I

I

V

Q

Q

S

S

-

F

-

V

T

P

P

P

T

V

D

Q

S

D

D

H

F

L

V

M

A

E

L

L

Q

M

L

M

I

V

D

D

A

A

C

A

E

K

G

G

A

A

-

S

-

A

A

A

E

R

I

V

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

K

K

F
x
D

K

A

S

P

G

R

E

I

P

S

V

I

S

T

A

A

R

R

A

L

I

I

A

A

R

D

C

L

I

L

N

Q

-

T

-

A

-

G

A

A

D

D

R

R

V

V

F

L

T

T

I

M

N

T

I

L

H
|
H

E

S

K

P

S

Q

V

V

L

H

E

G

H

F

F

F

P

K

C

I

P

P

A

V

E

D

N

H

L

L

D

S

A

A

A

E

A

P

L

V

I

I

G

A

S

N

Y

Y

V

F

A

A

G

T

-

R

F

V

G

D

L

L

E

E

R

N

P

A

M

V

L

V

V

V

A

A

P

P

D

D

E

A

G

G

A

D

R

L

G

K

L

R

V

A

K

S

N

A

V

L

A

A

S

R

G

R

H

L

G

G

F

L

D

P

H

L

D

A

H

F

L

I

Q

D

K

K

T

E

R
|
R

L

V

S

S

G

D

D

T

T

E

V

V

V

R

I

V

K

R

T

M

K

L

N

V

L

G

D

E

V

V

T

E

G

G

R

K

H

T

V

A

V

L

L

I

V

V

D

D

M

E

I

I

A

S

T

T

G

A

G

G

T

S

M

L

A

V

E

E

S

A

I

V

R

E

M

A

L

L

K

M

S

Q

Q

A

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

A

C

A

V

T

H

H

P

G

V

V

L

Y

T

V

R

G

N

P

A

A

A

L

L

D

R

R

L

I

F

A

H

K

A

S

G

P

V

V

K

K

D

E

I

V

I

A

G

A

T

T

D

D

T

T

L

C

E

P

K

P

A

K

E

E

S

G

-

P

-

K

-

L

-

R

K

T

L

L

S

T

V

V

A

A

P

P

L

L

I

F

A

A

E

E

A

A

L

I

binding adenosine-5'-diphosphate: R94 (= R101), S95 (≠ Q102), D99 (≠ F106), H128 (= H132), R193 (= R196)
binding sulfate ion: S45 (≠ E56), R47 (≠ E58)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
34% identity, 95% coverage: 13:292/295 of query aligns to 3:294/312 of 7pn0A

query
sites
7pn0A

L

L

K

L

I

I

I

F

G

S

G

G

P

Q

A

S

S

N

Q

R

L

P

L

L

A

A

S

Q

R

A

T

I

A

A

R

E

A

A

L

L

G

G

T

L

E

P

P

L

V

G

L

K

C

S

E

T

F

T

N

L

R

R

F

F

P

A

D

N

G

D

E

N

L

L

Y

F

L

V

R

R

I

Y

A

E

E

E

E

S

I

L

E

R

N

E

E

G

K

D

V

V

T

F

L

I

I

V

Q

Q

S

S

-

F

-

V

T

P

P

P

T

V

D

Q

S

D

D

H

F

L

V

M

A

E

L

L

Q

M

L

M

I

V

D

D

A

A

C

A

E

K

G

G

A

A

-

S

-

A

A

A

E

R

I

V

N

T

V

A

V

V

I

I

P

P

Y

Y

M

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

K

K

F

D

K

A

S

P

G

R

E

I

P

S

V

I

S

T

A

A

R

R

A

L

I

I

A

A

R

D

C

L

I

L

N

Q

-

T

-

A

-

G

A

A

D

D

R

R

V

V

F

L

T

T

I

M

N

T

I

L

H
|
H

E

S

K

P

S

Q

V

V

L

H

E

G

H

F

F

F

P

K

C

I

P

P

A

V

E

D

N

H

L

L

D

S

A

A

A

E

A

P

L

V

I

I

G

A

S

N

Y

Y

V

F

A

A

G

T

-

R

F

V

G

D

L

L

E

E

R

N

P

A

M

V

L

V

V

V

A

A

P

P

D

D

E

A

G

G

A

D

R

L

G

K

L

R

V

A

K

S

N

A

V

L

A

A

S

R

G

R

H

L

G

G

F

L

D

P

H

L

D

A

H

F

L

I

Q

D

K

K

T

E

R

R

L

V

S

S

G

D

D

T

T

E

V

V

V

R

I

V

K

R

T

M

K

L

N

V

L

G

D

E

V

V

T

E

G

G

R

K

H

T

V

A

V

L

L

I

V

V

D

D

M

E

I

I

A

S

T

T

G

A

G

G

T

S

M

L

A

V

E

E

S

A

I

V

R

E

M

A

L

L

K

M

S

Q

Q

A

G

G

A

A

I

K

D

E

V

V

H

Y

L

A

A

A

C

A

V

T

H

H

P

G

V

V

L

Y

T

V

R

G

N

P

A

A

A

L

L

D

R

R

L

I

F

A

H

K

A

S

G

P

V

V

K

K

D

E

I

V

I

A

G

A

T

T

D

D

T

T

L

C

E

P

K

P

A

K

E

E

S

G

-

P

-

K

-

L

-

R

K

T

L

L

S

T

V

V

A

A

P

P

L

L

I

F

A

A

E

E

A

A

L

I

binding adenosine-5'-diphosphate: R95 (= R101), S96 (≠ Q102), H129 (= H132)

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 95% coverage: 13:292/295 of query aligns to 12:309/326 of P9WKE3

query
sites
P9WKE3

L

L

K

M

I

L

I

F

G

A

G

G

P

R

A

A

S

H

Q

P

L

E

L

L

A

A

S

E

R

Q

T

V

A

A

R
x
K

A

E

L

L

G

D

T

V

E

H

P

V

V

T

L

S

C

Q

E

D

F

A

N

R

R

E

F

F

P

A

D

N

G

G

E

E

L

I

Y

F

L

V

R

R

I

F

A

H

E

E

E

S

I

V

E

R

N

G

E

C

K

D

V

A

T

F

L

V

I

L

Q

Q

S

S

T

C

P

P

T

A

D

P

S

V

D

N

F

R

V

W

A

L

L

M

L

E

Q

Q

L

L

I

I

-

M

-

I

D

D

A

A

C

L

E

K

-

R

-

G

G

S

A

A

A

K

E

R

I

I

N

T

V

A

V

V

I

M

P

P

Y

F

M

Y

G

P

Y

Y

A

A

R

R

Q

Q

D

D

K

K

F

H

K

R

S

G

G

R

E

E

P

P

V

I

S

S

A

A

R

R

A

L

I

I

A

A

-

D

-

L

R

K

C

T

I

A

N

G

A

A

D

D

R

R

V

I

F

V

T

T

I

V

N

D

I

L

H

H

E

T

K

D

S

Q

V

I

L

Q

E

G

H

F

F

F

P

D

C

G

P

P

A

V

E

D

N

H

L

M

D

R

A

G

A

Q

A

N

L

L

I

L

G

T

S

G

Y

Y

V

I

A

R

G

D

F

N

G

Y

L

P

E

D

R

G

P

N

M

M

L

V

V

V

-

V

A

S

P

P

D

D

E

S

G

G

A

R

R

V

G

R

L

I

V

A

K

E

N

K

V

W

A

A

S

D

G

A

-

L

H

G

G

G

F

V

D

P

H

L

D

A

H

F

L

I

Q

H

K

K

T

T

R

R

-

D

-

P

L

R

S

V

G

P

D

N

T

Q

V

V

I

S

K

N

T

R

K

V

N

V

L

G

D

D

V

V

T

A

G

G

R

R

H

T

V

C

V

V

L

L

V

I

D

D

M

M

I

I

A

D

T

T

G

G

T

T

M

I

A

A

E

G

S

A

I

V

R

A

M

L

L

L

K

H

S

N

Q

D

G

G

A

A

I

G

D

D

V

V

H

I

L

I

A

A

C

A

V

T

H

H

P

G

V

V

L

L

T

S

R

D

N

P

A

A

L

Q

R

R

L

L

F

A

H

S

A

C

G

G

V

A

K

R

D

E

I

V

I

I

G

V

T

T

D

N

T

T

L

L

E

P

K

I

A

G

E

E

S

D

K

K

-

R

-

F

-

P

-

Q

-

L

-

T

-

V

L

L

S

S

V

I

A

A

P

P

L

L

I

L

A

A

E

S

A

T

L

I

K29 (≠ R30) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>WP_011034136.1 NCBI__GCF_000007065.1:WP_011034136.1
MEYIKTATEVYILKIIGGPASQLLASRTARALGTEPVLCEFNRFPDGELYLRIAEEIENE
KVTLIQSTPTDSDFVALLQLIDACEGAAEINVVIPYMGYARQDKKFKSGEPVSARAIARC
INADRVFTINIHEKSVLEHFPCPAENLDAAALIGSYVAGFGLERPMLVAPDEGARGLVKN
VASGHGFDHDHLQKTRLSGDTVVIKTKNLDVTGRHVVLVDDMIATGGTMAESIRMLKSQG
AIDVHLACVHPVLTRNAALRLFHAGVKDIIGTDTLEKAESKLSVAPLIAEALSGY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory