SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (= H84) mutation to A: 5-fold decrease in glutamine affinity.
A105 (= A111) mutation to H: Little effect on the kinetic properties.
E349 (≠ D379) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
30% identity, 65% coverage: 24:437/634 of query aligns to 23:404/497 of 1ct9A

query
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1ct9A

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active site: L50 (= L53), N74 (= N78), G75 (= G79), T305 (≠ S357), R308 (vs. gap), E332 (≠ D379), M366 (= M416)
binding adenosine monophosphate: L232 (≠ F277), L233 (= L278), S234 (= S279), S239 (= S284), A255 (= A303), V256 (≠ I304), D263 (≠ E313), M316 (≠ V364), S330 (= S377), G331 (= G378), E332 (≠ D379)
binding glutamine: R49 (= R52), L50 (= L53), I52 (= I55), V53 (= V56), N74 (= N78), G75 (= G79), E76 (= E80), D98 (= D105)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
28% identity, 62% coverage: 1:395/634 of query aligns to 1:381/561 of P08243

query
sites
P08243

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C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (vs. gap) to E: in dbSNP:rs1049674
F362 (≠ L376) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 58% coverage: 24:388/634 of query aligns to 24:365/557 of P78753

query
sites
P78753

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L

I

-

R

-

A

-

S

-

T

P

P

T

M

F

Y

L

L

V

L

T

S

-

R

K

K

M

I

A

K

R

A

Q

Q

H

G

V

V

T

K

V

M

A

V

L

L

S

S

G

G

D

E

G

G

G

S

D

D

E

E

L

I

F

F

G

G

Y

Y

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
28% identity, 62% coverage: 2:395/634 of query aligns to 1:368/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

L

I

A
x
W

G

A

F

L

F

F

N

-

Y

-

Q

-

F

-

I

-

A

G

D

S

N

D

T

D

I

C

L

L

H

S

L

V

Q

Q

S

C

M

L

G

S

K

A

-

M

S

K

I

I

S

A

H

H

R

R

G

G

P

P

D

D

A

A

-

F

S

R

G

F

T

E

W

N

Y

V

D

N

A

G

D

Y

T

T

G

N

I

C

G

C

L

F

V

G

H

F

T

H

R
|
R

L
|
L

A

A

I
x
V

V
|
V

D

D

L

-

S

P

S

L

A

F

G

G

H

M

Q

Q

P

P

M

I

H

R

S

V

Q

K

C

K

D

Y

R

P

Y

Y

V

L

-

W

I

L

A

C

F
x
Y

N
|
N

G
|
G

E
|
E

I

I

Y

Y

N

N

H

H

L

K

M

K

L

M

R

Q

Q

Q

K

H

L

F

E

E

Q

F

A

E

N

Y

Q

Q

A

T

P

K

A
x
V

W

-

R

-

G

-

H

-

S

-

D
|
D

T

G

E

E

T

I

L

I

L

L

A

H

C

L

F

Y

V

D

A

K

W

G

G

G

I

I

E

E

Q

Q

T

T

L

I

K

C

V

M

T

L

V

D

G

G

M

V

F

F

A

A

L

F

A

V

L

L

W

L

D

D

K

T

Q

A

E

N

Q

K

N

K

L

V

T

F

L

L

A

G

R

R

D

D

R

T

A

Y

G

G

E

V

K

R

P

P

L

L

Y

F

W

K

G

A

W

M

Q

T

G

E

D

D

T

G

L

F

L

L

-

A

F

V

G

C

S

S

E

E

L

A

K

K

A

G

L

L

K

-

A

-

H

-

P

-

D

-

F

-

K

-

A

-

K

-

V

-

S

-

R

-

D

-

A

-

L

-

A

-

V

V

Y

T

M

L

R

K

H

H

N

S

Y

-

I

-

S

A

A

T

P

P

Q

-

S

-

I

-

Y

F

E

L

G

K

I

V

Y

E

K

P

L

F

L

L

P

P

G

G

H

H

Y

Y

V

E

T

V

L

L

N

D

S

L

V

K

S

P

N

N

I

G

D

K

T

V

A

A

T

-

P

-

K

-

A

-

Y

-

W

-

T

S

M

V

N

E

Q

M

A

V

I

K

E

Y

S

H

G

H

I

C

A

R

Q

D

P

V

F

F

S

P

G

G

T

F

D

E

I

I

E

E

A

T

V

V

-

K

D

N

L

N

L

L

E

R

H

I

R

L

L

F

S

N

Q

N

S

A

I

V

G

K

Q

R

M

L

L

M

A

T

D

D

V

R

P

R

L

I

G

G

A

C

F

L

L

L

S

S

G

G

V

L

D

D

S

S

V

L

V

V

V

A

A

A

L

T

M

L

Q

L

K

K

Q

Q

A

L

Q

K

Q

E

S

A

-

Q

-

V

-

Q

-

Y

-

P

V

L

K

Q

T

T

Y

F

A

A

I

I

G

G

F

M

D

E

D

D

P

S

R

-

Y

-

N

P

E

D

A

L

Q

L

Y

A

A

A

A

R

A

K

V

V

A

A

R

D

H

H

L

I

G

G

T

S

E

E

H

H

T

Y

E

E

L

V

Y

L

V

F

S

N

A

S

Q

E

D

E

A

G

L

I

E

Q

L

A

V

L

P

D

Q

E

L

V

P

-

S

-

I

I

Y

F

C

S

E

L

P

E

F

T

A

Y

D

D

-

I

-
x
T

-

T

-

V

S
x
R

S

A

Q

S

L

V

P

G

T

M

F

Y

L

L

V

I

T

S

K

K

M

Y

A

I

R

R

Q

K

H

N

V

T

-

D

-

S

T

V

V

V

A

I

L

F

S

S

G

G

D

E

G

G

G

S

D

D

E

E

L

L

F

T

G

Q

G

G

Y

Y

T

I

P

Y

Y

F

L

H

F

K

T

A

P

P

active site: W4 (≠ A5), L49 (= L53), N74 (= N78), G75 (= G79), T324 (vs. gap), R327 (≠ S356)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R52), V51 (≠ I55), V52 (= V56), Y73 (≠ F77), N74 (= N78), G75 (= G79), E76 (= E80), V95 (≠ A99), D96 (= D105)

1jgtB Crystal structure of beta-lactam synthetase (see paper)
29% identity, 43% coverage: 77:346/634 of query aligns to 72:307/500 of 1jgtB

query
sites
1jgtB

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

M

E

L

L

R

L

Q

S

K

V

L

L

E

-

Q

P

A

A

N

G

Q

P

A

A

P

P

A

-

W

-

R

-

G

-

H

E

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

C

L

F

L

V

E

A

R

W

Y

G

D

I

L

E

-

Q

H

T

A

L

F

K

R

V

L

T

V

V

N

G

G

M

R

F

F

A

A

L

T

A

V

L

V

W

-

D

-

K

R

Q

T

E

G

Q

D

N

R

L

V

T

L

L

L

A

A

R

T

D

D

R

H

A

A

G

G

E

S

K

V

P

P

L

L

Y

Y

W

T

G

C

W

V

Q

A

G

P

D

G

T

E

L

V

L

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

K

A

A

A

H

H

P

R

D

D

F

P

K

K

A

G

K

F

V

P

S

L

R

A

D

D

A

A

L

R

A

R

V

V

Y

-

M

-

R

-

H

-

N

-

Y

-

I

-

S

-

A

-

P

-

Q

-

S

A

I

G

Y

L

E

T

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

Y

V

V

M

T

D

L

I

N

D

S

L

V

G

S

S

N

G

I

-

D

-

T

T

A

A

T

V

P

T

K

H

A

R

Y

T

W

W

T

T

M

-

N

-

Q

-

A

-

I

-

E

-

S

P

G

G

I

L

A

S

Q

R

P

R

F

I

S

L

G

-

T

P

D

E

I

G

E

E

A

A

V

V

D

A

L

A

L

V

E

R

H

A

R

A

L

L

S

E

Q

K

S

A

I

V

G

A

G

Q

Q

R

M

V

L

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V
x
I

D
|
D

S
|
S

S

S

V

G

V

V

V

A

A

A

L

C

M

A

Q

H

K

R

Q

A

A

A

Q

G

Q

E

S

-

V

L

K

D

T

T

Y

V

A
x
S

I
x
M

G

G

F

T

D

D

D

T

P

-

R

-

Y

S

N

N

E

E

A

F

Q

R

Y

E

A

A

A

R

A

A

V

V

A

V

R

D

H

H

L

L

G

R

T

T

E

R

H

H

T

R

E

E

L

I

Y

T

V

I

S

P

A

T

Q

T

D

-

A

-

L

-

E

E

L

L

V

L

P

A

Q

Q

L

L

P

P

active site: A73 (≠ N78), G74 (= G79)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F277), L245 (= L278), S246 (= S279), G248 (= G281), I249 (≠ V282), D250 (= D283), S251 (= S284), S269 (≠ A303), M270 (≠ I304)
binding magnesium ion: D250 (= D283)

Sites not aligning to the query:

active site: 319, 345, 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 327, 344, 345, 348, 420, 440
binding n2-(carboxyethyl)-l-arginine: 323, 345, 346, 348, 349, 354, 370, 379
binding magnesium ion: 348

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
28% identity, 43% coverage: 77:346/634 of query aligns to 68:299/496 of 1mbzA

query
sites
1mbzA

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

M

E

L

L

R

L

Q

S

K

V

L

L

E

-

Q

P

A

A

N

G

Q

P

A

A

P

P

A

-

W

-

R

-

G

-

H

E

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

C

L

F

L

V

E

A

R

W

Y

G

D

I

L

E

-

Q

H

T

A

L

F

K

R

V

L

T

V

V

N

G

G

M

R

F

F

A

A

L

T

A

V

L

V

W

-

D

-

K

R

Q

T

E

G

Q

D

N

R

L

V

T

L

L

L

A

A

R

T

D

D

R

H

A

A

G

G

E

S

K

V

P

P

L

L

Y

Y

W

T

G

C

W

V

Q

A

G

P

D

G

T

E

L

V

L

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

K

A

A

A

H

H

P

G

D

F

F

P

K

L

A

A

K

D

V

A

S

R

R

R

D

V

A

A

L

-

A

-

V

-

Y

-

M

-

R

-

H

-

N

-

Y

-

I

-

S

-

A

-

P

-

Q

-

S

-

I

G

Y

L

E

T

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

Y

V

V

M

T

D

L

I

N

D

S

L

V

G

S

S

N

G

I

-

D

-

T

T

A

A

T

V

P

T

K

H

A

R

Y

T

W

W

T

T

M

-

N

-

Q

-

A

-

I

-

E

-

S

P

G

G

I

L

A

S

Q

R

P

R

F

I

S

L

G

-

T

P

D

E

I

G

E

E

A

A

V

V

D

A

L

A

L

V

E

R

H

A

R

A

L

L

S

E

Q

K

S

A

I

V

G

A

G

Q

Q

R

M

V

L

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

V

G

V

V

V

A

A

A

L

C

M

A

Q

H

K

R

Q

A

A

A

Q

G

Q

E

S

-

V

L

K

D

T

T

Y

V

A
x
S

I
x
M

G

G

F

T

D

D

D

T

P

-

R

-

Y

S

N

N

E

E

A

F

Q

R

Y

E

A

A

A

R

A

A

V

V

A

V

R

D

H

H

L

L

G

R

T

T

E

R

H

H

T

R

E

E

L

I

Y

T

V

I

S

P

A

T

Q

T

D

-

A

-

L

-

E

E

L

L

V

L

P

A

Q

Q

L

L

P

P

active site: A69 (≠ N78), G70 (= G79)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F277), L237 (= L278), S238 (= S279), S243 (= S284), S261 (≠ A303), M262 (≠ I304)
binding magnesium ion: D242 (= D283)
binding pyrophosphate 2-: S238 (= S279), G240 (= G281), D242 (= D283), S243 (= S284)

Sites not aligning to the query:

active site: 311, 337, 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 315, 319, 336, 337, 338, 340, 341, 362, 371, 432, 434, 435
binding magnesium ion: 340
binding pyrophosphate 2-: 340, 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
28% identity, 43% coverage: 77:346/634 of query aligns to 69:298/485 of 1mb9A

query
sites
1mb9A

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

M

E

L

L

R

L

Q

S

K

V

L

L

E

-

Q

P

A

A

N

G

Q

P

A

A

P

P

A

-

W

-

R

-

G

-

H

E

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

C

L

F

L

V

E

A

R

W

Y

G

D

I

L

E

-

Q

H

T

A

L

F

K

R

V

L

T

V

V

N

G

G

M

R

F

F

A

A

L

T

A

V

L

V

W

-

D

-

K

R

Q

T

E

G

Q

D

N

R

L

V

T

L

L

L

A

A

R

T

D

D

R

H

A

A

G

G

E

S

K

V

P

P

L

L

Y

Y

W

T

G

C

W

V

Q

A

G

P

D

G

T

E

L

V

L

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

K

A

A

A

H

F

P

P

D

-

F

-

K

-

A

-

K

-

V

-

S

-

R

-

D

-

A

-

L

L

A

A

V

D

Y

A

M

R

R

R

H

V

N

A

Y

G

I

L

S

T

A

-

P

-

Q

-

S

-

I

-

Y

-

E

-

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

Y

V

V

M

T

D

L

I

N

D

S

L

V

G

S

S

N

G

I

-

D

-

T

T

A

A

T

V

P

T

K

H

A

R

Y

T

W

W

T

T

M

-

N

-

Q

-

A

-

I

-

E

-

S

P

G

G

I

L

A

S

Q

R

P

R

F

I

S

L

G

-

T

P

D

E

I

G

E

E

A

A

V

V

D

A

L

A

L

V

E

R

H

A

R

A

L

L

S

E

Q

K

S

A

I

V

G

A

G

Q

Q

R

M

V

L

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

V

G

V

V

V

A

A

A

L

C

M

A

Q

H

K

R

Q

A

A

A

Q

G

Q

E

S

-

V

L

K

D

T

T

Y

V

A
x
S

I
x
M

G

G

F

T

D

D

D

T

P

-

R

-

Y

S

N

N

E

E

A

F

Q

R

Y

E

A

A

A

R

A

A

V

V

A

V

R

D

H

H

L

L

G

R

T

T

E

R

H

H

T

R

E

E

L

I

Y

T

V

I

S

P

A

T

Q

T

D

-

A

-

L

-

E

E

L

L

V

L

P

A

Q

Q

L

L

P

P

active site: A70 (≠ N78), G71 (= G79)
binding adenosine monophosphate: V235 (≠ F277), L236 (= L278), S242 (= S284), S260 (≠ A303), M261 (≠ I304)
binding adenosine-5'-triphosphate: V235 (≠ F277), L236 (= L278), S237 (= S279), G239 (= G281), D241 (= D283), S242 (= S284), S260 (≠ A303), M261 (≠ I304)
binding magnesium ion: D241 (= D283)
binding pyrophosphate 2-: S237 (= S279), G239 (= G281), D241 (= D283), S242 (= S284)

Sites not aligning to the query:

active site: 310, 336, 370, 431
binding adenosine monophosphate: 314, 318, 335, 336
binding adenosine-5'-triphosphate: 318, 335, 339, 411, 431
binding magnesium ion: 339
binding pyrophosphate 2-: 339, 411, 431

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
35% identity, 18% coverage: 274:387/634 of query aligns to 241:353/503 of Q9XB61

query
sites
Q9XB61

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G
|
G

V
x
L

D
|
D

S
|
S

S

S

V

L

V

V

V

T

A

A

L

L

M

A

Q

S

K

R

Q

H

A

F

Q

K

S

-

V

L

K

N

T

T

Y

Y

A

S

I
|
I

G

G

F

T

D

E

D

-

P

-

R

L

Y

S

N

N

E

E

A

F

Q

E

Y

F

A

S

A

Q

V

V

A

A

R

D

H

A

L

L

G

G

T

T

E

H

H

H

T

Q

E

M

L

K

Y

I

V

L

S

S

A

E

Q

T

D

E

A

V

L

I

E

N

L

G

V

I

P

-

Q

-

L

I

P

E

S

S

I

I

Y

Y

C

Y

E

N

P

E

F

I

A

F

D

D

-

G

-

L

S

S

S

A

Q

E

L

I

P

Q

T

S

-

G

-

L

F

F

L

N

V

V

T

Y

K

R

M

Q

A

A

R

Q

Q

G

H

Q

V

V

T

S

V

C

A

M

L

L

S

T

G
|
G

D
x
Y

G
|
G

G
x
S

D
|
D

E

L

L

L

F

F

G

G

244:251 (vs. 277:284, 75% identical) binding ATP
I270 (= I304) binding ATP
GYGSD 344:348 (≠ GDGGD 378:382) binding ATP
Y345 (≠ D379) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G380) binding substrate

Sites not aligning to the query:

371 binding substrate
374 binding substrate
380 E→A: Loss of activity.; E→D: Reduces catalytic efficiency.; E→Q: Reduces catalytic efficiency.
421 binding ATP
443 mutation K->A,M: Loss of activity.
444:446 binding ATP

1q19A Carbapenam synthetase (see paper)
35% identity, 18% coverage: 274:387/634 of query aligns to 240:352/500 of 1q19A

query
sites
1q19A

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G

G

V

L

D
|
D

S
|
S

S

S

V

L

V

V

V

T

A

A

L

L

M

A

Q

S

K

R

Q

H

A

F

Q

K

S

-

V

L

K

N

T

T

Y

Y

A
x
S

I
|
I

G

G

F

T

D

E

D

L

P

S

R

-

Y

-

N

N

E

E

A

F

Q

E

Y

F

A

S

A

Q

V

V

A

A

R

D

H

A

L

L

G

G

T

T

E

H

H

H

T

Q

E

M

L

K

Y

I

V

L

S

S

A

E

Q

T

D

E

A

V

L

I

E

N

L

G

V

I

P

-

Q

-

L

I

P

E

S

S

I

I

Y

Y

C

Y

E

N

P

E

F

I

A

F

D

D

-

G

-
x
L

S

S

S

A

Q
x
E

L

I

P

Q

T

S

-

G

-

L

F

F

L

N

V

V

T

Y

K

R

M

Q

A

A

R

Q

Q

G

H

Q

V

V

T

S

V

C

A

M

L

L

S
x
T

G
|
G

D
x
Y

G
|
G

G

S

D
|
D

E
x
L

L

L

F

F

G

G

active site: L318 (vs. gap), E321 (≠ Q358), Y344 (≠ D379)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ F277), L244 (= L278), S245 (= S279), D249 (= D283), S250 (= S284), S268 (≠ A303), I269 (= I304), T342 (≠ S377), G343 (= G378), D347 (= D382)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ D379), G345 (= G380), L348 (≠ E383)

Sites not aligning to the query:

active site: 56, 379, 442
binding diphosphomethylphosphonic acid adenosyl ester: 442, 443, 444, 445
binding (2s,5s)-5-carboxymethylproline: 373, 379

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
28% identity, 43% coverage: 77:346/634 of query aligns to 64:294/491 of 1mc1A

query
sites
1mc1A

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

M

E

L

L

R

L

Q

S

K

V

L

L

E

-

Q

P

A

A

N

G

Q

P

A

A

P

P

A

-

W

-

R

-

G

-

H

E

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

C

L

F

L

V

E

A

R

W

Y

G

D

I

L

E

-

Q

H

T

A

L

F

K

R

V

L

T

V

V

N

G

G

M

R

F

F

A

A

L

T

A

V

L

V

W

-

D

-

K

R

Q

T

E

G

Q

D

N

R

L

V

T

L

L

L

A

A

R

T

D

D

R

H

A

A

G

G

E

S

K

V

P

P

L

L

Y

Y

W

T

G

C

W

V

Q

A

G

P

D

G

T

E

L

V

L

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

K

A

A

A

H

-

P

-

D

G

F

F

K

P

A

L

K

A

V

D

S

A

R

R

D

R

A

V

L

A

A

G

V

L

Y

T

M

-

R

-

H

-

N

-

Y

-

I

-

S

-

A

-

P

-

Q

-

S

-

I

-

Y

-

E

-

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

Y

V

V

M

T

D

L

I

N

D

S

L

V

G

S

S

N

G

I

-

D

-

T

T

A

A

T

V

P

T

K

H

A

R

Y

T

W

W

T

T

M

-

N

-

Q

-

A

-

I

-

E

-

S

P

G

G

I

L

A

S

Q

R

P

R

F

I

S

L

G

-

T

P

D

E

I

G

E

E

A

A

V

V

D

A

L

A

L

V

E

R

H

A

R

A

L

L

S

E

Q

K

S

A

I

V

G

A

G

Q

Q

R

M

V

L

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L

L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

V

G

V

V

V

A

A

A

L

C

M

A

Q

H

K

R

Q

A

A

A

Q

G

Q

E

S

-

V

L

K

D

T

T

Y

V

A
x
S

I
x
M

G

G

F

T

D

D

D

T

P

-

R

-

Y

S

N

N

E

E

A

F

Q

R

Y

E

A

A

A

R

A

A

V

V

A

V

R

D

H

H

L

L

G

R

T

T

E

R

H

H

T

R

E

E

L

I

Y

T

V

I

S

P

A

T

Q

T

D

-

A

-

L

-

E

E

L

L

V

L

P

A

Q

Q

L

L

P

P

active site: A65 (≠ N78), G66 (= G79)
binding adenosine monophosphate: V231 (≠ F277), S233 (= S279), S238 (= S284), S256 (≠ A303), M257 (≠ I304)
binding magnesium ion: D237 (= D283)
binding pyrophosphate 2-: S233 (= S279), G235 (= G281), D237 (= D283), S238 (= S284)

Sites not aligning to the query:

active site: 306, 332, 366, 427
binding adenosine monophosphate: 331, 427, 430
binding magnesium ion: 335
binding deoxyguanidinoproclavaminic acid: 310, 332, 333, 336, 357, 366, 427
binding pyrophosphate 2-: 335, 407, 427, 428

Query Sequence

>WP_011187278.1 NCBI__GCF_000025945.1:WP_011187278.1
MCGLAGFFNYQFIADNTILHLQSMGKSISHRGPDASGTWYDADTGIGLVHTRLAIVDLSS
AGHQPMHSQCDRYVIAFNGEIYNHLMLRQKLEQANQAPAWRGHSDTETLLACFVAWGIEQ
TLKVTVGMFALALWDKQEQNLTLARDRAGEKPLYWGWQGDTLLFGSELKALKAHPDFKAK
VSRDALAVYMRHNYISAPQSIYEGIYKLLPGHYVTLNSVSNIDTATPKAYWTMNQAIESG
IAQPFSGTDIEAVDLLEHRLSQSIGGQMLADVPLGAFLSGGVDSSVVVALMQKQAQQSVK
TYAIGFDDPRYNEAQYAAAVARHLGTEHTELYVSAQDALELVPQLPSIYCEPFADSSQLP
TFLVTKMARQHVTVALSGDGGDELFGGYTPYLFTPRYWNLLKCYPQPLRAALAASMSRLP
LPDKFLKLAEVMGASNKESFYRSIMSHWGQPEDVVIGAKEPASVLNSSDLWPETDSFEHW
MMAMEAQMYMPDDILVKVDRAAMANSLETRVPLLDHRVIELAWQLPLSMKIRDGKGKWVL
REVLYRHVPRELIERPKKGFSIPLANWLRGPLRDWAEALLDEKRLRGEGYFYPAPIREAW
QQHVQGKRDNASKLWSVLMFQAWLDEQDANSITL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory