SitesBLAST
Comparing WP_011188115.1 NCBI__GCF_000025945.1:WP_011188115.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
28% identity, 91% coverage: 2:546/598 of query aligns to 2:507/539 of 6lpiB
- active site: I27 (= I27), G29 (= G29), G30 (≠ S30), S31 (≠ A31), I32 (≠ M32), E53 (= E52), C76 (≠ Q75), F115 (= F114), Q116 (= Q115), E117 (= E116), K165 (≠ R163), M256 (≠ Y254), A283 (≠ S281), V375 (≠ I408), G401 (= G434), M403 (≠ C436), D428 (= D461), N455 (= N489), A457 (≠ Q491), L458 (≠ W492), L460 (≠ A494), V461 (≠ E495), Q464 (≠ N498)
- binding flavin-adenine dinucleotide: R155 (= R152), G212 (= G208), G213 (≠ A209), G214 (= G210), T236 (≠ G234), L237 (≠ Y235), M238 (≠ Q236), L254 (= L252), M256 (≠ Y254), H257 (≠ N255), G276 (= G274), A277 (≠ T275), R278 (= R276), D280 (≠ N278), R282 (≠ F280), A283 (≠ S281), D300 (= D301), I301 (= I302), D319 (= D320), V320 (≠ A321), M380 (≠ A413), G398 (= G431)
- binding magnesium ion: D428 (= D461), N455 (= N489)
- binding thiamine diphosphate: E53 (= E52), C76 (≠ Q75), P79 (= P78), G376 (= G409), Q377 (≠ N410), H378 (≠ N411), G401 (= G434), M403 (≠ C436), G427 (= G460), D428 (= D461), G429 (= G462), S430 (≠ A463), M433 (≠ I466), N455 (= N489), A457 (≠ Q491), L458 (≠ W492), G459 (= G493), L460 (≠ A494), V461 (≠ E495)
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 14:561/599 of 6denA
- active site: Y35 (≠ I27), G37 (= G29), G38 (≠ S30), A39 (= A31), I40 (≠ M32), E61 (= E52), T84 (≠ Q75), F123 (= F114), Q124 (= Q115), E125 (= E116), K173 (≠ R163), K230 (= K218), M266 (≠ Y254), V293 (≠ S281), V409 (≠ I408), L434 (≠ F433), G435 (= G434), M437 (≠ C436), D462 (= D461), N489 (= N489), E491 (≠ Q491), Q492 (≠ W492), M494 (≠ A494), V495 (≠ E495), W498 (≠ N498), L520 (≠ I521), N525 (≠ G526), V526 (≠ L527)
- binding flavin-adenine dinucleotide: R163 (= R152), G219 (= G208), A220 (= A209), G221 (= G210), N224 (≠ L213), T246 (≠ G234), L247 (≠ Y235), Q248 (= Q236), L264 (= L252), G286 (= G274), A287 (≠ T275), R288 (= R276), D290 (≠ N278), R292 (≠ F280), V293 (≠ S281), E319 (≠ D301), I320 (= I302), N324 (≠ R306), D338 (= D320), V339 (≠ A321), M414 (≠ A413), G432 (= G431)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (≠ Y254), D291 (≠ P279), R292 (≠ F280), W498 (≠ N498)
- binding magnesium ion: D462 (= D461), N489 (= N489), E491 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (≠ I408), G410 (= G409), Q411 (≠ N410), H412 (≠ N411), G435 (= G434), M437 (≠ C436), G461 (= G460), D462 (= D461), A463 (≠ G462), S464 (≠ A463), N489 (= N489), E491 (≠ Q491), Q492 (≠ W492), G493 (= G493), M494 (≠ A494), V495 (≠ E495)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 12:559/597 of 6demA
- active site: Y33 (≠ I27), G35 (= G29), G36 (≠ S30), A37 (= A31), I38 (≠ M32), E59 (= E52), T82 (≠ Q75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (≠ R163), K228 (= K218), M264 (≠ Y254), V291 (≠ S281), V407 (≠ I408), L432 (≠ F433), G433 (= G434), M435 (≠ C436), D460 (= D461), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), M492 (≠ A494), V493 (≠ E495), W496 (≠ N498), L518 (≠ I521), N523 (≠ G526), V524 (≠ L527)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ Y254), D289 (≠ P279), R290 (≠ F280), M492 (≠ A494), W496 (≠ N498)
- binding flavin-adenine dinucleotide: R161 (= R152), G217 (= G208), A218 (= A209), G219 (= G210), N222 (≠ L213), T244 (≠ G234), L245 (≠ Y235), Q246 (= Q236), L262 (= L252), G284 (= G274), A285 (≠ T275), R286 (= R276), D288 (≠ N278), R290 (≠ F280), V291 (≠ S281), E317 (≠ D301), I318 (= I302), N322 (≠ R306), D336 (= D320), V337 (≠ A321), M412 (≠ A413), G430 (= G431)
- binding magnesium ion: D460 (= D461), N487 (= N489), E489 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ I408), G408 (= G409), Q409 (≠ N410), H410 (≠ N411), M435 (≠ C436), G459 (= G460), D460 (= D461), A461 (≠ G462), S462 (≠ A463), M465 (≠ I466), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), G491 (= G493), M492 (≠ A494), V493 (≠ E495)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 12:559/597 of 6delA
- active site: Y33 (≠ I27), G35 (= G29), G36 (≠ S30), A37 (= A31), I38 (≠ M32), E59 (= E52), T82 (≠ Q75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (≠ R163), K228 (= K218), M264 (≠ Y254), V291 (≠ S281), V407 (≠ I408), L432 (≠ F433), G433 (= G434), M435 (≠ C436), D460 (= D461), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), M492 (≠ A494), V493 (≠ E495), W496 (≠ N498), L518 (≠ I521), N523 (≠ G526), V524 (≠ L527)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ P279), R290 (≠ F280), W496 (≠ N498)
- binding flavin-adenine dinucleotide: R161 (= R152), G217 (= G208), A218 (= A209), G219 (= G210), N222 (≠ L213), T244 (≠ G234), L245 (≠ Y235), Q246 (= Q236), L262 (= L252), G284 (= G274), A285 (≠ T275), R286 (= R276), D288 (≠ N278), R290 (≠ F280), V291 (≠ S281), E317 (≠ D301), I318 (= I302), N322 (≠ R306), D336 (= D320), V337 (≠ A321), M412 (≠ A413), G430 (= G431)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ I408), G408 (= G409), Q409 (≠ N410), H410 (≠ N411), G433 (= G434), M435 (≠ C436), G459 (= G460), D460 (= D461), A461 (≠ G462), S462 (≠ A463), M465 (≠ I466), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), G491 (= G493), M492 (≠ A494), V493 (≠ E495)
- binding magnesium ion: D460 (= D461), N487 (= N489), E489 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ I408), G408 (= G409), Q409 (≠ N410), H410 (≠ N411), G433 (= G434), M435 (≠ C436), G459 (= G460), D460 (= D461), A461 (≠ G462), S462 (≠ A463), M465 (≠ I466), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), G491 (= G493), M492 (≠ A494), V493 (≠ E495)
6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 14:563/601 of 6deqA
- active site: Y35 (≠ I27), G37 (= G29), G38 (≠ S30), A39 (= A31), I40 (≠ M32), E61 (= E52), T84 (≠ Q75), F123 (= F114), Q124 (= Q115), E125 (= E116), K173 (≠ R163), K232 (= K218), M268 (≠ Y254), V295 (≠ S281), V411 (≠ I408), L436 (≠ F433), G437 (= G434), M439 (≠ C436), D464 (= D461), N491 (= N489), E493 (≠ Q491), Q494 (≠ W492), M496 (≠ A494), V497 (≠ E495), W500 (≠ N498), L522 (≠ I521), N527 (≠ G526), V528 (≠ L527)
- binding flavin-adenine dinucleotide: R163 (= R152), G221 (= G208), A222 (= A209), G223 (= G210), N226 (≠ L213), T248 (≠ G234), L249 (≠ Y235), Q250 (= Q236), L266 (= L252), G288 (= G274), A289 (≠ T275), R290 (= R276), D292 (≠ N278), R294 (≠ F280), V295 (≠ S281), E321 (≠ D301), I322 (= I302), D340 (= D320), V341 (≠ A321), M416 (≠ A413), G434 (= G431)
- binding magnesium ion: D464 (= D461), N491 (= N489), E493 (≠ Q491)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (≠ Y254), R294 (≠ F280), M496 (≠ A494), V497 (≠ E495), W500 (≠ N498)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (≠ I408), G412 (= G409), Q413 (≠ N410), H414 (≠ N411), M439 (≠ C436), G463 (= G460), D464 (= D461), A465 (≠ G462), S466 (≠ A463), N491 (= N489), E493 (≠ Q491), Q494 (≠ W492), G495 (= G493), M496 (≠ A494), V497 (≠ E495)
Sites not aligning to the query:
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 10:555/593 of 6deoA
- active site: Y31 (≠ I27), G33 (= G29), G34 (≠ S30), A35 (= A31), I36 (≠ M32), E57 (= E52), T80 (≠ Q75), F119 (= F114), Q120 (= Q115), E121 (= E116), K169 (≠ R163), K224 (= K218), M260 (≠ Y254), V287 (≠ S281), V403 (≠ I408), L428 (≠ F433), G429 (= G434), M431 (≠ C436), D456 (= D461), N483 (= N489), E485 (≠ Q491), Q486 (≠ W492), M488 (≠ A494), V489 (≠ E495), W492 (≠ N498), L514 (≠ I521), N519 (≠ G526), V520 (≠ L527)
- binding flavin-adenine dinucleotide: R159 (= R152), G213 (= G208), A214 (= A209), G215 (= G210), N218 (≠ L213), T240 (≠ G234), L241 (≠ Y235), Q242 (= Q236), L258 (= L252), G280 (= G274), A281 (≠ T275), R282 (= R276), D284 (≠ N278), R286 (≠ F280), V287 (≠ S281), E313 (≠ D301), I314 (= I302), N318 (≠ R306), D332 (= D320), V333 (≠ A321), M408 (≠ A413), G426 (= G431)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (≠ Y254), D285 (≠ P279), R286 (≠ F280), M488 (≠ A494), W492 (≠ N498)
- binding magnesium ion: D456 (= D461), N483 (= N489), E485 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (≠ I408), G404 (= G409), Q405 (≠ N410), H406 (≠ N411), G429 (= G434), M431 (≠ C436), G455 (= G460), D456 (= D461), A457 (≠ G462), S458 (≠ A463), M461 (≠ I466), N483 (= N489), E485 (≠ Q491), Q486 (≠ W492), G487 (= G493), M488 (≠ A494), V489 (≠ E495)
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 14:562/600 of 6derA
- active site: Y35 (≠ I27), G37 (= G29), G38 (≠ S30), A39 (= A31), I40 (≠ M32), E61 (= E52), T84 (≠ Q75), F123 (= F114), Q124 (= Q115), E125 (= E116), K173 (≠ R163), K231 (= K218), M267 (≠ Y254), V294 (≠ S281), V410 (≠ I408), L435 (≠ F433), G436 (= G434), M438 (≠ C436), D463 (= D461), N490 (= N489), E492 (≠ Q491), Q493 (≠ W492), M495 (≠ A494), V496 (≠ E495), W499 (≠ N498), L521 (≠ I521), N526 (≠ G526), V527 (≠ L527)
- binding flavin-adenine dinucleotide: R163 (= R152), G220 (= G208), A221 (= A209), G222 (= G210), N225 (≠ L213), T247 (≠ G234), L248 (≠ Y235), Q249 (= Q236), L265 (= L252), H268 (≠ N255), G287 (= G274), A288 (≠ T275), R289 (= R276), D291 (≠ N278), R293 (≠ F280), V294 (≠ S281), E320 (≠ D301), I321 (= I302), N325 (≠ R306), G338 (= G319), D339 (= D320), V340 (≠ A321), Q414 (≠ C412), M415 (≠ A413), G433 (= G431)
- binding Metosulam: R293 (≠ F280), M495 (≠ A494), W499 (≠ N498)
- binding magnesium ion: D463 (= D461), N490 (= N489), E492 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (≠ I408), G411 (= G409), Q412 (≠ N410), H413 (≠ N411), G436 (= G434), M438 (≠ C436), G462 (= G460), D463 (= D461), A464 (≠ G462), S465 (≠ A463), N490 (= N489), E492 (≠ Q491), Q493 (≠ W492), G494 (= G493), M495 (≠ A494), V496 (≠ E495)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (≠ I408), G411 (= G409), Q412 (≠ N410), H413 (≠ N411), G436 (= G434), M438 (≠ C436), G462 (= G460), D463 (= D461), A464 (≠ G462), S465 (≠ A463), M468 (≠ I466), N490 (= N489), E492 (≠ Q491), Q493 (≠ W492), G494 (= G493), V496 (≠ E495)
Sites not aligning to the query:
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 12:560/598 of 6desA
- active site: Y33 (≠ I27), G35 (= G29), G36 (≠ S30), A37 (= A31), I38 (≠ M32), E59 (= E52), T82 (≠ Q75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (≠ R163), K229 (= K218), M265 (≠ Y254), V292 (≠ S281), V408 (≠ I408), L433 (≠ F433), G434 (= G434), M436 (≠ C436), D461 (= D461), N488 (= N489), E490 (≠ Q491), Q491 (≠ W492), M493 (≠ A494), V494 (≠ E495), W497 (≠ N498), L519 (≠ I521), N524 (≠ G526), V525 (≠ L527)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (≠ Y254), D290 (≠ P279), R291 (≠ F280), W497 (≠ N498)
- binding flavin-adenine dinucleotide: R161 (= R152), G218 (= G208), A219 (= A209), G220 (= G210), N223 (≠ L213), T245 (≠ G234), L246 (≠ Y235), Q247 (= Q236), L263 (= L252), G285 (= G274), A286 (≠ T275), R287 (= R276), D289 (≠ N278), R291 (≠ F280), V292 (≠ S281), E318 (≠ D301), I319 (= I302), N323 (≠ R306), D337 (= D320), V338 (≠ A321), Q412 (≠ C412), M413 (≠ A413), G431 (= G431)
- binding magnesium ion: D461 (= D461), N488 (= N489), E490 (≠ Q491)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ I408), G409 (= G409), Q410 (≠ N410), H411 (≠ N411), G434 (= G434), M436 (≠ C436), G460 (= G460), D461 (= D461), A462 (≠ G462), S463 (≠ A463), N488 (= N489), E490 (≠ Q491), Q491 (≠ W492), G492 (= G493), M493 (≠ A494), V494 (≠ E495)
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
26% identity, 94% coverage: 6:566/598 of query aligns to 12:560/598 of 6depA
- active site: Y33 (≠ I27), G35 (= G29), G36 (≠ S30), A37 (= A31), I38 (≠ M32), E59 (= E52), T82 (≠ Q75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (≠ R163), K229 (= K218), M265 (≠ Y254), V292 (≠ S281), V408 (≠ I408), L433 (≠ F433), G434 (= G434), M436 (≠ C436), D461 (= D461), N488 (= N489), E490 (≠ Q491), Q491 (≠ W492), M493 (≠ A494), V494 (≠ E495), W497 (≠ N498), L519 (≠ I521), N524 (≠ G526), V525 (≠ L527)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (≠ P279), R291 (≠ F280), M493 (≠ A494), W497 (≠ N498)
- binding flavin-adenine dinucleotide: R161 (= R152), G218 (= G208), A219 (= A209), G220 (= G210), N223 (≠ L213), T245 (≠ G234), L246 (≠ Y235), Q247 (= Q236), L263 (= L252), G264 (= G253), G285 (= G274), A286 (≠ T275), R287 (= R276), D289 (≠ N278), R291 (≠ F280), V292 (≠ S281), E318 (≠ D301), I319 (= I302), N323 (≠ R306), D337 (= D320), V338 (≠ A321), M413 (≠ A413), G431 (= G431)
- binding magnesium ion: D461 (= D461), N488 (= N489), E490 (≠ Q491)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (≠ I408), G409 (= G409), Q410 (≠ N410), H411 (≠ N411), G434 (= G434), M436 (≠ C436), G460 (= G460), D461 (= D461), A462 (≠ G462), S463 (≠ A463), M466 (≠ I466), N488 (= N489), E490 (≠ Q491), Q491 (≠ W492), G492 (= G493), M493 (≠ A494), V494 (≠ E495)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ I408), G409 (= G409), Q410 (≠ N410), H411 (≠ N411), G434 (= G434), M436 (≠ C436), G460 (= G460), D461 (= D461), A462 (≠ G462), S463 (≠ A463), M466 (≠ I466), N488 (= N489), E490 (≠ Q491), Q491 (≠ W492), G492 (= G493), M493 (≠ A494), V494 (≠ E495)
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 8:544/595 of 1t9bB
- active site: Y29 (≠ I27), G31 (= G29), G32 (≠ S30), A33 (= A31), I34 (≠ M32), E55 (= E52), T78 (≠ Q75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (≠ R163), R226 (≠ K218), M262 (≠ Y254), V289 (≠ S281), V405 (≠ I408), L430 (≠ F433), G431 (= G434), M433 (≠ C436), D458 (= D461), N485 (= N489), E487 (≠ Q491), Q488 (≠ W492), M490 (≠ A494), V491 (≠ E495), W494 (≠ N498), L516 (≠ I521), G521 (= G526), L522 (= L527)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (≠ A104), P108 (≠ A105), D287 (≠ P279), R288 (≠ F280), M490 (≠ A494), W494 (≠ N498)
- binding flavin-adenine dinucleotide: R157 (≠ S154), G215 (= G208), A216 (= A209), G217 (= G210), N220 (vs. gap), T242 (≠ G234), L243 (≠ Y235), Q244 (= Q236), M259 (≠ P251), L260 (= L252), M262 (≠ Y254), H263 (≠ N255), G282 (= G274), A283 (≠ T275), R284 (= R276), D286 (≠ N278), R288 (≠ F280), V289 (≠ S281), E315 (≠ D301), V316 (≠ I302), N320 (≠ R306), G333 (= G319), D334 (= D320), A335 (= A321), Q409 (≠ C412), M410 (≠ A413), G428 (= G431), G429 (≠ M432)
- binding magnesium ion: D458 (= D461), N485 (= N489), E487 (≠ Q491)
Sites not aligning to the query:
1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 8:532/583 of 1t9bA
- active site: Y29 (≠ I27), G31 (= G29), G32 (≠ S30), A33 (= A31), I34 (≠ M32), E55 (= E52), T78 (≠ Q75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (≠ R163), R214 (≠ K218), M250 (≠ Y254), V277 (≠ S281), V393 (≠ I408), L418 (≠ F433), G419 (= G434), M421 (≠ C436), D446 (= D461), N473 (= N489), E475 (≠ Q491), Q476 (≠ W492), M478 (≠ A494), V479 (≠ E495), W482 (≠ N498), L504 (≠ I521), G509 (= G526), L510 (= L527)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (≠ A104), P108 (≠ A105), F117 (= F114), D275 (≠ P279), R276 (≠ F280), M478 (≠ A494), W482 (≠ N498)
- binding flavin-adenine dinucleotide: R157 (≠ S154), G203 (= G208), A204 (= A209), G205 (= G210), N208 (vs. gap), T230 (≠ G234), L231 (≠ Y235), Q232 (= Q236), M247 (≠ P251), L248 (= L252), M250 (≠ Y254), H251 (≠ N255), G270 (= G274), A271 (≠ T275), R272 (= R276), D274 (≠ N278), R276 (≠ F280), V277 (≠ S281), E303 (≠ D301), V304 (≠ I302), N308 (≠ R306), D322 (= D320), A323 (= A321), Q397 (≠ C412), M398 (≠ A413), G416 (= G431), G417 (≠ M432)
- binding magnesium ion: D446 (= D461), N473 (= N489), E475 (≠ Q491)
Sites not aligning to the query:
1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 7:531/582 of 1t9dB
- active site: Y28 (≠ I27), G30 (= G29), G31 (≠ S30), A32 (= A31), I33 (≠ M32), E54 (= E52), T77 (≠ Q75), F116 (= F114), Q117 (= Q115), E118 (= E116), K166 (≠ R163), R213 (≠ K218), M249 (≠ Y254), V276 (≠ S281), V392 (≠ I408), L417 (≠ F433), G418 (= G434), M420 (≠ C436), D445 (= D461), N472 (= N489), E474 (≠ Q491), Q475 (≠ W492), M477 (≠ A494), V478 (≠ E495), W481 (≠ N498), L503 (≠ I521), G508 (= G526), L509 (= L527)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (≠ S30), A32 (= A31), V106 (≠ A104), P107 (≠ A105), F116 (= F114), K166 (≠ R163), M249 (≠ Y254), D274 (≠ P279), R275 (≠ F280), W481 (≠ N498)
- binding flavin-adenine dinucleotide: R156 (≠ S154), G202 (= G208), A203 (= A209), G204 (= G210), N207 (vs. gap), T229 (≠ G234), L230 (≠ Y235), Q231 (= Q236), L247 (= L252), M249 (≠ Y254), H250 (≠ N255), G269 (= G274), A270 (≠ T275), R271 (= R276), D273 (≠ N278), R275 (≠ F280), V276 (≠ S281), E302 (≠ D301), V303 (≠ I302), N307 (≠ R306), G320 (= G319), D321 (= D320), A322 (= A321), Q396 (≠ C412), M397 (≠ A413), G415 (= G431), G416 (≠ M432)
- binding magnesium ion: D445 (= D461), N472 (= N489), E474 (≠ Q491)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E54 (= E52), P80 (= P78), G418 (= G434), M420 (≠ C436), M450 (≠ I466)
Sites not aligning to the query:
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 10:548/599 of 1n0hA
- active site: Y31 (≠ I27), G33 (= G29), G34 (≠ S30), A35 (= A31), I36 (≠ M32), E57 (= E52), T80 (≠ Q75), F119 (= F114), Q120 (= Q115), E121 (= E116), K169 (≠ R163), R230 (≠ K218), M266 (≠ Y254), V293 (≠ S281), V409 (≠ I408), L434 (≠ F433), G435 (= G434), M437 (≠ C436), D462 (= D461), N489 (= N489), E491 (≠ Q491), Q492 (≠ W492), M494 (≠ A494), V495 (≠ E495), W498 (≠ N498), L520 (≠ I521), G525 (= G526), L526 (= L527)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (≠ I408), G410 (= G409), Q411 (≠ N410), H412 (≠ N411), G435 (= G434), M437 (≠ C436), G461 (= G460), D462 (= D461), A463 (≠ G462), S464 (≠ A463), M467 (≠ I466), N489 (= N489), E491 (≠ Q491), Q492 (≠ W492), G493 (= G493), V495 (≠ E495)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (≠ S30), A35 (= A31), V109 (≠ A104), P110 (≠ A105), F119 (= F114), K169 (≠ R163), M266 (≠ Y254), D291 (≠ P279), R292 (≠ F280), V495 (≠ E495), W498 (≠ N498)
- binding flavin-adenine dinucleotide: R159 (≠ S154), G219 (= G208), A220 (= A209), G221 (= G210), N224 (vs. gap), T246 (≠ G234), L247 (≠ Y235), Q248 (= Q236), L264 (= L252), G265 (= G253), M266 (≠ Y254), H267 (≠ N255), G286 (= G274), A287 (≠ T275), R288 (= R276), D290 (≠ N278), R292 (≠ F280), V293 (≠ S281), E319 (≠ D301), V320 (≠ I302), N324 (≠ R306), G337 (= G319), D338 (= D320), A339 (= A321), M414 (≠ A413), G432 (= G431), G433 (≠ M432)
- binding magnesium ion: D462 (= D461), N489 (= N489), E491 (≠ Q491)
- binding thiamine diphosphate: Y31 (≠ I27), E57 (= E52), P83 (= P78)
Sites not aligning to the query:
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 8:545/596 of 1t9cA
- active site: Y29 (≠ I27), G31 (= G29), G32 (≠ S30), A33 (= A31), I34 (≠ M32), E55 (= E52), T78 (≠ Q75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (≠ R163), R227 (≠ K218), M263 (≠ Y254), V290 (≠ S281), V406 (≠ I408), L431 (≠ F433), G432 (= G434), M434 (≠ C436), D459 (= D461), N486 (= N489), E488 (≠ Q491), Q489 (≠ W492), M491 (≠ A494), V492 (≠ E495), W495 (≠ N498), L517 (≠ I521), G522 (= G526), L523 (= L527)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ S30), V107 (≠ A104), P108 (≠ A105), F117 (= F114), K167 (≠ R163), D288 (≠ P279), R289 (≠ F280), W495 (≠ N498)
- binding flavin-adenine dinucleotide: R157 (≠ S154), G216 (= G208), A217 (= A209), G218 (= G210), N221 (vs. gap), T243 (≠ G234), L244 (≠ Y235), Q245 (= Q236), L261 (= L252), M263 (≠ Y254), H264 (≠ N255), G283 (= G274), A284 (≠ T275), R285 (= R276), D287 (≠ N278), R289 (≠ F280), V290 (≠ S281), E316 (≠ D301), V317 (≠ I302), N321 (≠ R306), G334 (= G319), D335 (= D320), A336 (= A321), M411 (≠ A413), G429 (= G431), G430 (≠ M432)
- binding magnesium ion: D459 (= D461), N486 (= N489), E488 (≠ Q491)
Sites not aligning to the query:
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 8:545/596 of 1t9dA
- active site: Y29 (≠ I27), G31 (= G29), G32 (≠ S30), A33 (= A31), I34 (≠ M32), E55 (= E52), T78 (≠ Q75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (≠ R163), R227 (≠ K218), M263 (≠ Y254), V290 (≠ S281), V406 (≠ I408), L431 (≠ F433), G432 (= G434), M434 (≠ C436), D459 (= D461), N486 (= N489), E488 (≠ Q491), Q489 (≠ W492), M491 (≠ A494), V492 (≠ E495), W495 (≠ N498), L517 (≠ I521), G522 (= G526), L523 (= L527)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ S30), A33 (= A31), V107 (≠ A104), P108 (≠ A105), F117 (= F114), K167 (≠ R163), M263 (≠ Y254), D288 (≠ P279), R289 (≠ F280), W495 (≠ N498)
- binding flavin-adenine dinucleotide: R157 (≠ S154), G216 (= G208), A217 (= A209), G218 (= G210), N221 (vs. gap), T243 (≠ G234), L244 (≠ Y235), Q245 (= Q236), M260 (≠ P251), L261 (= L252), H264 (≠ N255), G283 (= G274), A284 (≠ T275), R285 (= R276), D287 (≠ N278), R289 (≠ F280), V290 (≠ S281), E316 (≠ D301), V317 (≠ I302), N321 (≠ R306), G334 (= G319), D335 (= D320), A336 (= A321), Q410 (≠ C412), M411 (≠ A413), G429 (= G431), G430 (≠ M432)
- binding magnesium ion: D459 (= D461), N486 (= N489), E488 (≠ Q491)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E52), P81 (= P78), Q118 (= Q115), G432 (= G434), M434 (≠ C436), M464 (≠ I466)
Sites not aligning to the query:
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 9:546/597 of 1t9aA
- active site: Y30 (≠ I27), G32 (= G29), G33 (≠ S30), A34 (= A31), I35 (≠ M32), E56 (= E52), T79 (≠ Q75), F118 (= F114), Q119 (= Q115), E120 (= E116), K168 (≠ R163), R228 (≠ K218), M264 (≠ Y254), V291 (≠ S281), V407 (≠ I408), L432 (≠ F433), G433 (= G434), M435 (≠ C436), D460 (= D461), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), M492 (≠ A494), V493 (≠ E495), W496 (≠ N498), L518 (≠ I521), G523 (= G526), L524 (= L527)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (≠ S30), V108 (≠ A104), P109 (≠ A105), F118 (= F114), K168 (≠ R163), M264 (≠ Y254), D289 (≠ P279), R290 (≠ F280), M492 (≠ A494), V493 (≠ E495), W496 (≠ N498)
- binding flavin-adenine dinucleotide: R158 (≠ S154), G217 (= G208), A218 (= A209), G219 (= G210), N222 (vs. gap), T244 (≠ G234), L245 (≠ Y235), Q246 (= Q236), L262 (= L252), M264 (≠ Y254), H265 (≠ N255), G284 (= G274), A285 (≠ T275), R286 (= R276), D288 (≠ N278), R290 (≠ F280), V291 (≠ S281), E317 (≠ D301), V318 (≠ I302), N322 (≠ R306), G335 (= G319), D336 (= D320), A337 (= A321), Q411 (≠ C412), M412 (≠ A413), G430 (= G431), G431 (≠ M432)
- binding magnesium ion: D460 (= D461), N487 (= N489), E489 (≠ Q491)
- binding propyl trihydrogen diphosphate: V407 (≠ I408), G408 (= G409), Q409 (≠ N410), H410 (≠ N411), M435 (≠ C436), G459 (= G460), D460 (= D461), A461 (≠ G462), S462 (≠ A463), N487 (= N489), E489 (≠ Q491), Q490 (≠ W492), G491 (= G493), M492 (≠ A494)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G434), M435 (≠ C436), M465 (≠ I466)
Sites not aligning to the query:
5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 8:540/591 of 5wkcA
- active site: Y29 (≠ I27), G31 (= G29), G32 (≠ S30), A33 (= A31), I34 (≠ M32), E55 (= E52), T78 (≠ Q75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (≠ R163), R222 (≠ K218), M258 (≠ Y254), V285 (≠ S281), V401 (≠ I408), L426 (≠ F433), G427 (= G434), M429 (≠ C436), D454 (= D461), N481 (= N489), E483 (≠ Q491), Q484 (≠ W492), M486 (≠ A494), V487 (≠ E495), W490 (≠ N498), L512 (≠ I521), G517 (= G526), L518 (= L527)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (≠ I408), G402 (= G409), Q403 (≠ N410), H404 (≠ N411), G427 (= G434), M429 (≠ C436), G453 (= G460), D454 (= D461), A455 (≠ G462), S456 (≠ A463), M459 (≠ I466), N481 (= N489), E483 (≠ Q491), Q484 (≠ W492), G485 (= G493), M486 (≠ A494), V487 (≠ E495)
- binding ethaneperoxoic acid: G32 (≠ S30), Q118 (= Q115)
- binding flavin-adenine dinucleotide: R157 (≠ S154), G211 (= G208), A212 (= A209), G213 (= G210), N216 (vs. gap), T238 (≠ G234), L239 (≠ Y235), Q240 (= Q236), L256 (= L252), M258 (≠ Y254), G278 (= G274), A279 (≠ T275), R280 (= R276), R284 (≠ F280), V285 (≠ S281), E311 (≠ D301), V312 (≠ I302), N316 (≠ R306), D330 (= D320), A331 (= A321), M406 (≠ A413), G424 (= G431)
- binding magnesium ion: D454 (= D461), N481 (= N489), E483 (≠ Q491)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (≠ S30), A33 (= A31), V107 (≠ A104), F117 (= F114), K167 (≠ R163), M258 (≠ Y254), R284 (≠ F280), M486 (≠ A494), W490 (≠ N498)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: P30 (≠ I28), E55 (= E52)
Sites not aligning to the query:
6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 12:556/607 of 6u9dB
- active site: Y33 (≠ I27), G35 (= G29), G36 (≠ S30), A37 (= A31), I38 (≠ M32), E59 (= E52), T82 (≠ Q75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (≠ R163), M274 (≠ Y254), V301 (≠ S281), V417 (≠ I408), G443 (= G434), M445 (≠ C436), D470 (= D461), N497 (= N489), E499 (≠ Q491), Q500 (≠ W492), M502 (≠ A494), V503 (≠ E495), W506 (≠ N498)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (≠ S30), V111 (≠ A104), P112 (≠ A105), F121 (= F114), K171 (≠ R163), D299 (≠ P279), R300 (≠ F280), M502 (≠ A494), W506 (≠ N498)
- binding flavin-adenine dinucleotide: R161 (≠ S154), A228 (= A209), G229 (= G210), N232 (vs. gap), T254 (≠ G234), L255 (≠ Y235), Q256 (= Q236), L272 (= L252), M274 (≠ Y254), G294 (= G274), R296 (= R276), D298 (≠ N278), R300 (≠ F280), V301 (≠ S281), E327 (≠ D301), V328 (≠ I302), N332 (≠ R306), D346 (= D320), A347 (= A321), M422 (≠ A413), G440 (= G431), G441 (≠ M432)
- binding magnesium ion: D470 (= D461), N497 (= N489)
- binding thiamine diphosphate: E59 (= E52), P85 (= P78), V417 (≠ I408), G418 (= G409), Q419 (≠ N410), H420 (≠ N411), G443 (= G434), M445 (≠ C436), A471 (≠ G462), S472 (≠ A463), N497 (= N489), E499 (≠ Q491), Q500 (≠ W492), G501 (= G493), M502 (≠ A494), V503 (≠ E495)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 91% coverage: 6:549/598 of query aligns to 92:636/687 of P07342
- R241 (≠ S154) binding FAD
- 355:376 (vs. 255:276, 27% identical) binding FAD
- 407:426 (vs. 301:320, 20% identical) binding FAD
6dekA Crystal structure of candida albicans acetohydroxyacid synthase catalytic subunit (see paper)
25% identity, 94% coverage: 6:566/598 of query aligns to 13:557/595 of 6dekA
- active site: Y34 (≠ I27), G36 (= G29), G37 (≠ S30), A38 (= A31), I39 (≠ M32), E60 (= E52), T83 (≠ Q75), Q118 (= Q115), E119 (= E116), K167 (≠ R163), K226 (= K218), M262 (≠ Y254), V289 (≠ S281), V405 (≠ I408), L430 (≠ F433), G431 (= G434), M433 (≠ C436), D458 (= D461), N485 (= N489), E487 (≠ Q491), Q488 (≠ W492), M490 (≠ A494), V491 (≠ E495), W494 (≠ N498), L516 (≠ I521), N521 (≠ G526), V522 (≠ L527)
- binding flavin-adenine dinucleotide: R157 (= R152), G215 (= G208), A216 (= A209), G217 (= G210), N220 (≠ L213), T242 (≠ G234), L243 (≠ Y235), Q244 (= Q236), L260 (= L252), M262 (≠ Y254), G282 (= G274), A283 (≠ T275), R284 (= R276), D286 (≠ N278), R288 (≠ F280), V289 (≠ S281), E315 (≠ D301), I316 (= I302), N320 (≠ R306), D334 (= D320), V335 (≠ A321), M410 (≠ A413), G428 (= G431)
- binding magnesium ion: D458 (= D461), N485 (= N489), E487 (≠ Q491)
- binding thiamine diphosphate: V405 (≠ I408), G406 (= G409), Q407 (≠ N410), H408 (≠ N411), M433 (≠ C436), G457 (= G460), D458 (= D461), A459 (≠ G462), S460 (≠ A463), N485 (= N489), E487 (≠ Q491), Q488 (≠ W492), G489 (= G493), M490 (≠ A494), V491 (≠ E495)
Query Sequence
>WP_011188115.1 NCBI__GCF_000025945.1:WP_011188115.1
MTKMKMTTEEAFITVLQMHGIEHAFGIIGSAMMPISDLFPKAGITFWDCAHEGNAGMMAD
GYTRASGKMSMMVAQNGPGITNFITPVKTAYWNHTPLLLVTPQAANATMGQGGFQEVEQM
AVFKDMVAYQEEVRDPRRMAEVLNRVIMQAKRASAPAQINIPRDYWTQVIEIELPAIVDF
ERPAGGEKALEKAAELLSKAKFPVILNGAGVILGGAIKDTIELAERLDAPVCCGYQHNDA
FPGSHPLAAGPLGYNGSKAGMELIAQADVVLALGTRLNPFSTLPGYGIDYWPQNAAIIQV
DINPDRIGLTKAVKVGIIGDAKKVARGILNRLTATAGDTDREIRKANIGKTKAEWSVELA
NLVHEEDDPGTSWNKRARDREPDKMSPREAWNAMKSVIPENAIISSDIGNNCAIGNAYPT
FEEGRKYLAPGMFGPCGYGFPAICGAKIAQPDVPVIGMAGDGAFGISMNEMVSCGRKDWP
PITMIIFRNYQWGAEKRNTTLWYSDNFVGTELDPNVSYAKIAQACGLIGVQATCTDDLTA
QLKEAVRVQMEEGKTTFIEVLVNQELGEPFRRDAMKAPVMVAGIKKEDMRPQCCCCKK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory