SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011189344.1 NCBI__GCF_000025945.1:WP_011189344.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

P72578 2-oxoacid:ferredoxin oxidoreductase subunit alpha; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see 2 papers)
31% identity, 98% coverage: 7:561/566 of query aligns to 3:610/632 of P72578

query
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P72578

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Y253 (= Y220) mutation Y->A,W: Loss of oxidoreductase activity.; mutation to F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
YPITP 253:257 (≠ YPMTP 220:224) YPITP motif
P254 (= P221) mutation to G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
I255 (≠ M222) mutation I->L,M,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
T256 (= T223) mutation T->A,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
P257 (= P224) mutation P->A,G,V: Loss of oxidoreductase activity.
R344 (= R299) mutation R->A,K: Loss of oxidoreductase activity.
T353 (= T308) Plays a key role in the broad substrate specificity; mutation T->I,V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.

Q96Y66 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
30% identity, 98% coverage: 7:561/566 of query aligns to 3:610/627 of Q96Y66

query
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Q96Y66

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S41 (= S45) mutation to A: Same oxidoreductase activity as the wild-type.
T349 (= T304) mutation to L: Same oxidoreductase activity as the wild-type.
D468 (= D421) mutation to A: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.

5b48A 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
31% identity, 98% coverage: 7:561/566 of query aligns to 3:562/576 of 5b48A

query
sites
5b48A

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binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: Y221 (= Y220), P222 (= P221), I223 (≠ M222), E262 (= E249), R312 (= R299)

Q96XT2 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
30% identity, 98% coverage: 10:563/566 of query aligns to 7:613/628 of Q96XT2

query
sites
Q96XT2

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T257 (= T223) binding substrate
R344 (= R299) binding substrate

5b47A 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
30% identity, 98% coverage: 10:563/566 of query aligns to 6:612/627 of 5b47A

query
sites
5b47A

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I

R

K

G

G

G

R

I

H

N

S

T

Y

T

F

E

E

I

V

R

V

I

I

S

S

S

E

E

K

P

P

V

I

R

R

A

S

-

L

-

S

-

S

H

Y

V

V

D

N

V

I

-

L

-

A

-

S

-

F

-

D

-

A

-

E

-

T

-

V

-

F

-

Q

-

H

-

F

-

T

-

E

-

T

-

K

-

E

-

Y

-

L

-

I

-

Y

-

N

-

V

-

E

-

Y

-

E

-

N

-

T

-

T

I

V

D

D

L

L

L

V

I

K

P

S

L

M

K

E

N

P

G

E

V

M

I

A

E

E

H

Q

L

V

G

K

S

E

R

A

V

L

T

S

K

K

S

E

T

R

-

L

-

G

-

F

T

T

I

I

V

K

G

D

S

V

R

L

E

E

H

Y

I

L

G

K

G

R

R

R

D

G

T

V

E

K

I

V

-

I

-

G

-

F

D

N

F

Y

A

T

A

E

T

L

A

I

K

K

K

K

H

I

G

A

N

D

Q

T

L

F

Y

K

T

V

-

P

-

M

-

S

-

V

-

V

-

E

-

R

-

A

-

K

N

N

S

M

V

I

A

A

L

V

G

G

F

A

L

S

C

Y

G

G

L

L

L

L

D

G

F

L

D

K

S

F

A

D

N

Y

L

L

Y

K

K

D

E

A

I

I

R

S

D

S

T

T

F

F

S

-

L

-

K

K

S

N

E

E

G

L

I

F

I

I

A

K

G

F

N

N

I

T

D

M

A

A

A

A

E

E

E

L

G

G

Y

Y

K

-

I

-

G

-

M

-

A

-

S

-

H

-

S

-

R

-

L

-

L

-

T

-

S

N

G

S

R

V

P

P

T

N

P

V

A

Y

K

K

N

L

R

Q

S

E

L

Y

Q

K

-

I

-

E

-

K

E

Q

N

R

L

I

L

Q

L

V

N

D

G

G

A

N

D

T

A

I

V

S

A

A

L

M

G

G

A

K

M

L

A

A

G

G

G

G

C

L

N

R

A

F

A

Q

F

S

A

Y

Y
|
Y

P
|
P

M
x
I

T
|
T

P

P

G

A

T

S

-

D

-

E

S

S

V

V

F

Y

L

I

T

-

L

-

A

-

E

E

F

A

S

N

K

Q

Q

N

A

L

D

D

I

M

V

I

V

V

E

E

-

G

-

N

-

E

-

L

-

R

-

K

-

G

-

G

-

V

-

V

-

V

-

V

Q

Q

V

A

E

E

D

D

E
|
E

I

L

G

A

V

A

I

I

N

N

M

M

A

A

I

V

G

G

S

A

W

A

Y

L

A

T

G

G

G

V

R

R

A

S

I

A

V

T

S

A

T

T

S

S

G

G

G
x
P

G

G

F

F

A

S

L

L

M

M

T

S

E

E

G

G

I

I

S

S

L

W

A

A

G

G

M

M

T

N

E

E

T

V

P

P

V

V

V

V

V

I

H

T

L

Y

A

Y

Q

M

R
|
R

P

G

G

A

P

P

A

A

T

T

G

G

L

L

P
|
P

T

T

R

R

T

S

E

G

Q

Q

G

A

D

D

L

L

N

K

L

F

V

A

L

L

Y

N

A

V

G

G

H

H

G

G

E

E

F

F

A

P

R

R

L

I

I

V

L

I

A

A

P

S

G

G

T

D

L

H

E

V

Q

E

A

I

F

F

E

W

Y

D

T

A

R

I

L

W

A

A

F

L

N

N

L

L

A

A

D

E

R

K

F

Y

Q

Q

I

T

P

P

V

V

F

I

V

H

L

I

T

I

D

E

Q

K

F

T

F

L

V

A

D

N

S

A

Y

Y

Y

S

P

V

I

F

A

E

H

E

-

E

-

L

-

I

P

T

D

N

A

R

G

P

Q

Y

V

V

Q

I

I

E

E

R

S

G

A

K

V

I

I

V

K

K

-

P

T

T

D

S

D

D

S

Y

Y

F

K

N

R

R

Y

F

Q

E

L

V

S

T

E

E

D

D

G

G

V

I

S

S

P

P

R

R

G

V

I

F

P

L

G

G

Y

Q

G

A

S

S

G

-

N

-

V

I

C

F

L

Y

D

T

S

G

D

D

E

E

H

H

D

N

E

E

D

E

G

G

K

H

I

I

T

T

E

E

D

N

L

S

D

I

G

N

I

-

H

R

L

M

A

K

M

M

A

Y

D

E

N

K

R

R

L

N

K

K

K

K

L

L

E

E

T

T

L

A

T

D

K

K

A

E

A

-

M

-

L

I

P

P

E

E

-

E

-

Q

-

R

-

V

-

N

L

I

V

V

G

G

E

D

P

A

D

D

Y

-

A

-

T

I

L

V

I

L

I

L

G

T

W

W

G

G

S

S

T

P

Y

K

G

G

A

A

I

I

C

L

E

D

A

A

L

M

Q

E

I

E

I

L

G

S

S

K

G

D

K

G

V

I

S

K

F

T

-

M

-

M

-

V

-

Q

-

V

-

K

L

M

F

F

C

N

P

P

Q

Y

L

P

Y

K

P

N

L

L

S

M

E

K

K

K

I

I

A

-

E

-

Y

-

I

L

D

S

G

G

A

K

Q

S

K

K

V

I

V

I

V

A

V

V

E

E

N

N

N

N

Q

Y

L

N

G

A

Q

Q

F

G

A

A

D

E

F

V

L

L

Q

A

R

E

E

K

L

T

G

G

R

I

L

F

F

A

P

T

H

N

R

Y

I

I

L

L

K

K

Y

W

N

T

G

G

M

R

P

P

F

I

S

T

V

R

E

E

E

E

L

V

V

I

Q

E

S

G

I

I

T

K

E

K

binding pyruvic acid: T256 (= T223), R343 (= R299), P351 (= P307)
binding thiamine diphosphate: Y253 (= Y220), P254 (= P221), I255 (≠ M222), E293 (= E249), P317 (≠ G273)

5b46A 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
30% identity, 98% coverage: 10:563/566 of query aligns to 6:612/627 of 5b46A

query
sites
5b46A

V

M

L

I

G

G

G

G

E

A

A

Q

G

G

Q

L

G

G

I

V

Q

D

F

T

L

S

E

A

K

N

A

I

L

F

L

G

H

N

I

A

V

V

K

A

E

K

S

A

G

G

L

Y

Y

Y

V

L

F

F

A

G

T

N

K

R

E

E

Y

Y

M

Y

S

S

R

N

V

I

R

K

G

G

G

R

I

H

N

S

T

Y

T

F

E

E

I

V

R

V

I

I

S

S

S

E

E

K

P

P

V

I

R

R

A

S

-

L

-

S

-

S

H

Y

V

V

D

N

V

I

-

L

-

A

-

S

-

F

-

D

-

A

-

E

-

T

-

V

-

F

-

Q

-

H

-

F

-

T

-

E

-

T

-

K

-

E

-

Y

-

L

-

I

-

Y

-

N

-

V

-

E

-

Y

-

E

-

N

-

T

-

T

I

V

D

D

L

L

L

V

I

K

P

S

L

M

K

E

N

P

G

E

V

M

I

A

E

E

H

Q

L

V

G

K

S

E

R

A

V

L

T

S

K

K

S

E

T

R

-

L

-

G

-

F

T

T

I

I

V

K

G

D

S

V

R

L

E

E

H

Y

I

L

G

K

G

R

R

R

D

G

T

V

E

K

I

V

-

I

-

G

-

F

D

N

F

Y

A

T

A

E

T

L

A

I

K

K

K

K

H

I

G

A

N

D

Q

T

L

F

Y

K

T

V

-

P

-

M

-

S

-

V

-

V

-

E

-

R

-

A

-

K

N

N

S

M

V

I

A

A

L

V

G

G

F

A

L

S

C

Y

G

G

L

L

L

L

D

G

F

L

D

K

S

F

A

D

N

Y

L

L

Y

K

K

D

E

A

I

I

R

S

D

S

T

T

F

F

S

-

L

-

K

K

S

N

E

E

G

L

I

F

I

I

A

K

G

F

N

N

I

T

D

M

A

A

A

A

E

E

E

L

G

G

Y

Y

K

-

I

-

G

-

M

-

A

-

S

-

H

-

S

-

R

-

L

-

L

-

T

-

S

N

G

S

R

V

P

P

T

N

P

V

A

Y

K

K

N

L

R

Q

S

E

L

Y

Q

K

-

I

-

E

-

K

E

Q

N

R

L

I

L

Q

L

V

N

D

G

G

A

N

D

T

A

I

V

S

A

A

L

M

G

G

A

K

M

L

A

A

G

G

G

G

C

L

N

R

A

F

A

Q

F

S

A

Y

Y
|
Y

P
|
P

M
x
I

T

T

P

P

G

A

T

S

-

D

-

E

S

S

V

V

F

Y

L

I

T

-

L

-

A

-

E

E

F

A

S

N

K

Q

Q

N

A

L

D

D

I

M

V

I

V

V

E

E

-

G

-

N

-

E

-

L

-

R

-

K

-

G

-

G

-

V

-

V

-

V

-

V

Q

Q

V

A

E

E

D

D

E
|
E

I

L

G

A

V

A

I

I

N

N

M

M

A

A

I

V

G

G

S

A

W

A

Y

L

A

T

G

G

G

V

R

R

A

S

I

A

V

T

S

A

T

T

S

S

G

G

G

P

G

G

F

F

A

S

L

L

M

M

T

S

E

E

G

G

I

I

S

S

L

W

A

A

G

G

M

M

T

N

E

E

T

V

P

P

V

V

V

V

V

I

H

T

L

Y

A

Y

Q

M

R

R

P

G

G

A

P

P

A

A

T

T

G

G

L

L

P

P

T

T

R

R

T

S

E

G

Q

Q

G

A

D

D

L

L

N

K

L

F

V

A

L

L

Y

N

A

V

G

G

H

H

G

G

E

E

F

F

A

P

R

R

L

I

I

V

L

I

A

A

P

S

G

G

T

D

L

H

E

V

Q

E

A

I

F

F

E

W

Y

D

T

A

R

I

L

W

A

A

F

L

N

N

L

L

A

A

D

E

R

K

F

Y

Q

Q

I

T

P

P

V

V

F

I

V

H

L

I

T

I

D

E

Q

K

F

T

F

L

V

A

D

N

S

A

Y

Y

Y

S

P

V

I

F

A

E

H

E

-

E

-

L

-

I

P

T

D

N

A

R

G

P

Q

Y

V

V

Q

I

I

E

E

R

S

G

A

K

V

I

I

V

K

K

-

P

T

T

D

S

D

D

S

Y

Y

F

K

N

R

R

Y

F

Q

E

L

V

S

T

E

E

D

D

G

G

V

I

S

S

P

P

R

R

G

V

I

F

P

L

G

G

Y

Q

G

A

S

S

G

-

N

-

V

I

C

F

L

Y

D

T

S

G

D

D

E

E

H

H

D

N

E

E

D

E

G

G

K

H

I

I

T

T

E

E

D

N

L

S

D

I

G

N

I

-

H

R

L

M

A

K

M

M

A

Y

D

E

N

K

R

R

L

N

K

K

K

K

L

L

E

E

T

T

L

A

T

D

K

K

A

E

A

-

M

-

L

I

P

P

E

E

-

E

-

Q

-

R

-

V

-

N

L

I

V

V

G

G

E

D

P

A

D

D

Y

-

A

-

T

I

L

V

I

L

I

L

G

T

W

W

G

G

S

S

T

P

Y

K

G

G

A

A

I

I

C

L

E

D

A

A

L

M

Q

E

I

E

I

L

G

S

S

K

G

D

K

G

V

I

S

K

F

T

-

M

-

M

-

V

-

Q

-

V

-

K

L

M

F

F

C

N

P

P

Q

Y

L

P

Y

K

P

N

L

L

S

M

E

K

K

K

I

I

A

-

E

-

Y

-

I

L

D

S

G

G

A

K

Q

S

K

K

V

I

V

I

V

A

V

V

E

E

N

N

N

N

Q

Y

L

N

G

A

Q

Q

F

G

A

A

D

E

F

V

L

L

Q

A

R

E

E

K

L

T

G

G

R

I

L

F

F

A

P

T

H

N

R

Y

I

I

L

L

K

K

Y

W

N

T

G

G

M

R

P

P

F

I

S

T

V

R

E

E

E

E

L

V

V

I

Q

E

S

G

I

I

T

K

E

K

binding thiamine diphosphate: Y253 (= Y220), P254 (= P221), I255 (≠ M222), E293 (= E249)

6n2oC 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
31% identity, 99% coverage: 5:565/566 of query aligns to 3:571/572 of 6n2oC

query
sites
6n2oC

K

K

G

D

F

L

T

I

L

I

V

R

L

V

G

A

G

G

E

E

A

G

G
|
G

Q

E

G
|
G

I
|
I

Q
x
I

F
x
S

L

S

E

G

K

D

A

F

L

I

L

A

H

A

I

A

V

C

K

A

E

R

S

A

G

G

L

L

Y

E

V

V

F

Y

A

T

T

F

K

K

E

T

Y
x
F

M

P

S
x
A

R
x
E

V
x
I

R

K

G

G

G

G

I

Y

N

A

T

M

T

Y

E

Q

I

V

R

R

I

A

S

S

S

S

E

E

P

K

V

L

R

Y

A

C

H

Q

V

G

D

D

V

T

I

F

D

D

L

V

L

F

I

C

P

A

L

F

K

N

N

G

G

E

V

A

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binding succinyl-coenzyme a: G14 (= G16), G16 (= G18), I17 (= I19), I18 (≠ Q20), S19 (≠ F21), F41 (≠ Y43), A43 (≠ S45), E44 (≠ R46), I45 (≠ V47), R128 (≠ L121), N131 (= N124), F157 (= F150), N168 (= N161), T226 (= T223), R302 (= R299)
binding thiamine diphosphate: P224 (= P221), I225 (≠ M222), E252 (= E249)

6n2oA 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
31% identity, 99% coverage: 5:565/566 of query aligns to 3:571/572 of 6n2oA

query
sites
6n2oA

K

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