SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011189412.1 NCBI__GCF_000025945.1:WP_011189412.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
30% identity, 99% coverage: 1:365/368 of query aligns to 1:385/386 of P0A9J8

query
sites
P0A9J8

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R11 (= R11) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R28) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K39) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E52) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (= Q87) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
32% identity, 72% coverage: 91:354/368 of query aligns to 3:273/278 of 2qmxA

query
sites
2qmxA

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active site: T169 (= T259), F171 (= F261)
binding phenylalanine: N204 (≠ D285), E205 (≠ R286), L209 (= L290), D222 (≠ N303), L223 (= L304), T224 (≠ A305), K225 (= K306), S228 (= S309), Y238 (= Y319), F240 (= F321)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
33% identity, 71% coverage: 94:354/368 of query aligns to 5:274/306 of 3mwbA

query
sites
3mwbA

Y

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active site: T176 (= T259), F178 (= F261)
binding phenylalanine: D205 (= D285), H206 (≠ R286), L210 (= L290), N223 (= N303), L224 (= L304), S225 (≠ A305), R226 (≠ K306), S229 (= S309), F241 (= F321)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
33% identity, 71% coverage: 94:354/368 of query aligns to 5:271/303 of 3mwbB

query
sites
3mwbB

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active site: T176 (= T259), F178 (= F261)
binding magnesium ion: A155 (= A238), Q158 (≠ T241), L161 (= L244)
binding phenylalanine: D205 (= D285), H206 (≠ R286), L210 (= L290), N223 (= N303), L224 (= L304), S225 (≠ A305), R226 (≠ K306), I227 (= I307), Y236 (= Y319), F238 (= F321)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
29% identity, 64% coverage: 118:354/368 of query aligns to 34:276/282 of 6vh5D

query
sites
6vh5D

P

P

L

C

E

P

T

T

I

F

D

E

E

D

V

A

F

F

A

N

E

A

V

V

E

E

K

T

G

G

R

A

V

A

Q

D

Y

L

G

A

I

M

V

I

P

P

V

I

E

E

N

N

S

T

I

L

E

A

G

G

A

R

V

V

F

A

S

D

T

I

L

H

D

Y

S

L

F

L

M

P

K

L

Y

A

K

D

I

M

K

H

I

I

C

V

G

G

E

E

M

Y

Q

F

L

L

A

P

I

I

S

H

H

F

N

Q

L

L

V

M

C

V

K

L

S

P

G

G

-

V

N

R

I

R

E

E

D

E

I

I

Q

K

T

T

V

V

A

H

S

S

H

H

N

I

Q

H

P

A

L

L

A

G

Q

Q

C

C

R

R

D

N

W

V

L

I

R

R

K

Q

H

N

L

-

P

-

N

G

T

W

P

K

T

G

L

V

P

I

V

A

F

G

S

D

T

T

G

A

L

G

A

A

K

R

M

L

A

V

A

A

D

D

-

V

-

K

N

D

P

R

N

S

I

M

G

A

A

A

I

L

A

A

S

P

S

R

L

L

A

A

I

A

S

D

T

L

Y

Y

D

G

L

L

Q

D

V

I

V

L

V

E

K

E

G

N

I

V

E

E

D

D

Y

S

E

E

G

N

N

N

T

V

T

T

R

R

F

F

L

V

I

V

I

L

G

S

K

K

E

N

S

K

-

Q

-

W

-

A

-

R

P

P

G

E

I

N

S

D

G

E

R

R

D

I

R

V

T

T

S

T

L

F

L

V

I

F

G

R

L

V

M

R

D
x
N

R
x
V

P

P

G

A

A

A

L
|
L

N

Y

E

K

I

A

L

L

S

G

V

G

L

F

S

A

E

T

E

N

G

G

I

V

N
|
N

L
x
M

A
x
T

K
|
K

I

L

E

E

S
|
S

R

Y

P

Q

I

L

K

G

G

G

K

R

Q

F

W

I

K

A

Y
x
T

L

Q

F
|
F

F

Y

L

A

D

D

M

I

I

E

G

G

H

H

I

P

E

E

D

E

E

R

Q

S

I

V

K

Q

R

L

G

A

C

L

A

E

R

E

L

L

K

R

Q

F

E

F

C

T

S

K

Y

E

F

V

E

R

W

I

L

L

G

G

S

V

Y

Y

binding phenylalanine: N207 (≠ D285), V208 (≠ R286), L212 (= L290), N225 (= N303), M226 (≠ L304), T227 (≠ A305), K228 (= K306), S231 (= S309), T241 (≠ Y319), F243 (= F321)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
25% identity, 72% coverage: 94:359/368 of query aligns to 8:288/326 of 3luyA

query
sites
3luyA

Y

Y

L

L

G

G

P

P

E

Q

A

G

T

T

F

F

S

T

H

H

L

Q

A

A

G

A

V

V

-

N

-

A

-

Q

-

E

-

L

-

A

K

R

Y

F

F

E

G

P

H

Q

S

G

T

F

D

D

Y

L

K

M

P

P

L

M

E

D

T

D

I

V

D

P

E

Q

V

I

F

L

A

D

E

A

V

A

E

Q

K

H

G

G

R

D

V

-

Q

G

Y

W

G

G

I

I

V

V

P

A

V

W

E

E

N

N

S

N

I

V

E

E

G

G

A

Y

V

V

F

V

S

P

T

N

L

L

D

D

S

A

F

L

M

I

K

D

Y

A

K

K

I

-

K

D

I

L

C

V

G

G

E

F

M

A

Q

R

L

V

A

G

I

V

S

N

H

V

N

E

L

F

-

D

-

A

-

Y

V

V

C

A

K

Q

S

G

G

A

N

D

I

P

E

A

D

E

I

A

Q

R

T

I

V

A

A

T

S

A

H

H

N

P

Q

H

P

G

L

L

A

A

Q

Q

C

C

R

K

D

R

W

F

L

I

R

A

K

E

H

H

L

-

P

-

N

R

T

L

P

S

T

T

L

Q

P

P

V

A

F

T

S

S

T

N

G

A

L

A

A

A

A

C

K

R

M

D

A

L

A

I

D

-

N

-

P

P

N

G

I

E

G

I

A

A

I

F

A

G

S

P

S

A

L

I

A

C

I

G

S

E

T

L

Y

Y

D

D

L

I

Q

T

V

R

V

I

V

G

K

T

G

A

I

I

E

Q

D

D

Y

Y

E

Q

G

G

N

A

T

A

T
|
T

R

D

F

F

L

L

I

V

I

L

G

S

-

P

-

R

K

A

E

E

S

V

P

A

G

R

I

L

S

L

G

A

R

K

D

P

R

R

T

A

S

E

-

A

-

N

-

V

-

E

-

Y

-

E

-

S

-

V

-

L

L

T

L

L

I

I

G

P

L

L

M

V

D

T

R
x
G

P

P

G

G

A

V

L
|
L

N

A

E

N

I

L

L

L

S

D

V

V

L

F

S

R

E

D

E

A

G

G

I

L

N

N

L

M

A

T

K

S

I

F

E

I

S

S

R

R

P

P

I

I

K

K

G

G

K

R

Q

T

W

G

K

T

Y
|
Y

L

S

F
|
F

F

I

L

V

D

T

M

L

I

D

G

A

H

A

I

P

E

W

D

E

E

E

Q

R

I

F

K

R

R

D

G

A

C

L

A

V

R

E

L

I

K

A

Q

E

E

H

C

G

S

D

Y

W

F

A

E

K

W

T

L

L

G

A

S

V

Y

Y

P

P

Q

R

D

R

E

E

N

H

active site: T177 (= T259)
binding 3-phenylpyruvic acid: G215 (≠ R286), L219 (= L290), Y248 (= Y319), F250 (= F321)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
29% identity, 72% coverage: 91:355/368 of query aligns to 3:270/278 of 7am0B

query
sites
7am0B

V

I

A

A

Y

F

L

Q

G

G

P

R

E

P

A

G

T

A

F

Y

S

S

H

D

L

L

A

A

G

C

V

R

K

Q

Y

A

F

R

G

P

H

G

S

W

T

T

D

T

Y

L

K

-

P

P

L

C

E

Q

T

T

I

F

D

A

E

Q

V

T

F

I

A

A

E

A

V

V

E

H

K

D

G

G

R

R

V

A

Q

E

Y

L

G

A

I

M

V

L

P

A

V

C

E

E

N

N

S

S

I

L

E

A

G

G

A

R

V

V

F

P

S

D

T

I

L

H

D

A

S

L

F

L

M

P

K

E

Y

A

K

G

I

L

K

F

I

I

C

V

G

G

E

E

M

H

Q

F

L

Q

A

R

I

V

S

E

H

H

N

C

L

L

V

L

C

G

K

I

S

P

G

G

N

S

-

T

I

L

E

A

D

D

I

A

Q

R

T

R

V

I

A

H

S

T

H

H

N

P

Q

V

P

A

L

M

A

A

Q

Q

C

V

R

R

D

G

W

I

L

I

R

T

K

E

H

-

L

L

P

G

N

L

T

D

P

P

T

V

L

V

P

E

V

-

F

F

S

D

T

T

G

A

L

G

A

A

K

E

M

M

A

V

A

R

D

E

-

W

-

G

N

R

P

K

N

E

I

D

G

V

A

A

I

V

A

A

S

S

S

A

L

L

A

A

I

A

S

E

T

L

Y

N

D

G

L

L

Q

E

V

I

V

L

V

R

K

R

G

N

I

V

E

E

D

D

Y

A

E

T

G

H

N

N

T

T

R

R

F

F

L

Y

I

I

I

A

G

S

K

R

E

R

-

P

-

A

-

T

-

L

-

P

S

G

P

P

G

G

I

F

S

M

G

-

R

-

D

-

R

-

T

T

S

T

L

L

I

F

G

R

L

V

M
x
N

D
x
N

R
x
Q

P
|
P

G

G

A

A

L
|
L

N

Y

E

K

I

A

L

L

S

G

V

G

L

L

S

A

E

T

E

A

G

G

I

V

N
|
N

L
x
M

A
x
T

K
x
R

I

L

E

E

S

S

R

Y

P

M

I

L

K

E

G

G

K

S

Q

F

W

S

K

A

Y
x
T

L

Q

F
|
F

F

L

L

M

D

D

M

V

I

E

G

G

H

H

I

P

E

E

D

A

E

P

Q

P

I

L

K

-

R

-

G

-

C

-

A

A

R

R

L

A

K

L

Q

D

E

E

C

L

S

S

Y

F

F

F

-

S

-

E

-

Q

E

E

W

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: N199 (≠ M284), N200 (≠ D285), Q201 (≠ R286), P202 (= P287), L205 (= L290), N218 (= N303), M219 (≠ L304), T220 (≠ A305), R221 (≠ K306), T234 (≠ Y319), F236 (= F321)

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
38% identity, 19% coverage: 9:79/368 of query aligns to 1:66/345 of 3nvtA

query
sites
3nvtA

K

E

V

L

R

R

E

T

S

Q

I

V

D

D

T

Q

I

L

D

N

N

I

S

D

I

L

L

L

E

E

L

L

L

I

K

S

K

K

R

R

I

A

G

N

L

L

A

V

K

Q

E

E

I

I

G

G

R

K

L

I

K

K

S

G

E

-

D

-

N

-

R

-

S

-

K

R

W

F

D

D

P

P

L

L

R

R

E

E

R

R

E

E

I

M

Y

L

E

N

R

T

L

I

I

L

T

A

S

A

N

N

E

E

G

G

D

P

F

F

P

E

D

D

E

S

S

T

L

V

R

Q

S

K

I

L

F

F

H

K

E

E

I

I

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
39% identity, 19% coverage: 10:79/368 of query aligns to 1:65/343 of 3tfcA

query
sites
3tfcA

V

L

R

R

E

T

S

Q

I

V

D

D

T

Q

I

L

D

N

N

I

S

D

I

L

L

L

E

E

L

L

L

I

K

S

K

K

R

R

I

A

G

N

L

L

A

V

K

Q

E

E

I

I

G

G

R

K

L

I

K

K

S

L

E

-

D

-

N

-

R

-

S

-

K

R

W

F

D

D

P

P

L

L

R

R

E

E

R

R

E

E

I

M

Y

L

E

N

R

T

L

I

I

L

T

A

S

A

N

N

E

E

G

G

D

P

F

F

P

E

D

D

E

S

S

T

L

V

R

Q

S

K

I

L

F

F

H

K

E

E

I

I

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
34% identity, 19% coverage: 10:79/368 of query aligns to 9:78/358 of P39912

query
sites
P39912

V

L

R

R

E

Q

S

K

I

A

D

D

T

E

I

L

D

N

N

L

S

Q

I

I

L

L

E

K

L

L

L

I

K

N

K

E

R

R

I

G

G

N

L

V

A

V

K

K

E

E

I

I

G

G

R

K

L

A

K

K

S

E

E

A

D

Q

N

G

R

V

S

N

K

R

W

F

D

D

P

P

L

V

R

R

E

E

R

R

E

T

I

M

Y

L

E

N

R

N

L

I

I

I

T

E

S

N

N

N

E

D

G

G

D

P

F

F

P

E

D

N

E

S

S

T

L

I

R

Q

S

H

I

I

F

F

H

K

E

E

I

I

Sites not aligning to the query:

2 modified: Phosphoserine

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
30% identity, 22% coverage: 4:83/368 of query aligns to 1:80/352 of 5j6fA

query
sites
5j6fA

D

N

S

E

N

R

I

L

P

D

K

E

V

L

R

R

E

A

S

R

I

V

D

D

T

E

I

I

D

N

N

L

S

Q

I

L

L

L

E

K

L

L

L

I

K

N

K

E

R
|
R

I

G

G

R

L

L

A

V

K

Q

E

E

I
|
I

G

G

R

K

L

I

K
|
K

S

E

E

A

D

Q

N

G

R

T

S

H

K

R

W
x
Y

D
|
D

P

P

L

V

R
|
R

E

E

R

R

E

K

I
x
M

Y

L

E

D

R

L

L

I

I

S

T

E

S

H

N

N

E

D

G

G

D

P

F

F

P

E

D

T

E

S

S

T

L

L

R

Q

S

H

I

I

F

F

H

K

E

E

I

I

I

F

T

K

T

A

C

A

binding prephenic acid: R25 (= R28), I32 (= I35), K36 (= K39), Y44 (≠ W47), D45 (= D48), R48 (= R51), M52 (≠ I55)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
34% identity, 19% coverage: 10:79/368 of query aligns to 8:77/87 of 5gmuB

query
sites
5gmuB

V

L

R

R

E

Q

S

K

I

A

D

D

T

E

I

L

D

N

N

L

S

Q

I

I

L

L

E

K

L

L

L

I

K

N

K

E

R
|
R

I

G

G

N

L

V

A

V

K

K

E

E

I

I

G

G

R

K

L

A

K

K

S

E

E

A

D

Q

N

G

R

V

S

N

K
x
R

W
x
F

D
|
D

P

P

L

V

R

R

E
|
E

R

R

E

T

I

M

Y

L

E

N

R

N

L

I

I

I

T

E

S

N

N

N

E

D

G

G

D

P

F

F

P

E

D

N

E

S

S

T

L

I

R

Q

S

H

I

I

F

F

H

K

E

E

I

I

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R28), R44 (≠ K46), F45 (≠ W47), D46 (= D48), E50 (= E52)

Sites not aligning to the query:

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: 78, 79, 82, 85

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
34% identity, 27% coverage: 257:355/368 of query aligns to 85:186/194 of 7alzA

query
sites
7alzA

N

N

T

T

R

R

F

F

L

Y

I

I

I

A

G

S

K

R

E

R

S

P

-

A

-

T

-

L

P

P

G

P

I

P

S

G

G

P

R

G

D

F

R

M

T

T

S

T

L

L

I

F

G

R

L

V

M

N

D

N

R
x
Q

P

P

G

G

A

A

L
|
L

N

Y

E

K

I

A

L

L

S

G

V

G

L

L

S

A

E

T

E

A

G

G

I

V

N
|
N

L
x
M

A
x
T

K
x
R

I

L

E

E

S

S

R

Y

P

M

I

L

K

E

G

G

K

S

Q

F

W

S

K

A

Y
x
T

L

Q

F
|
F

F

L

L

M

D

D

M

V

I

E

G

G

H

H

I

P

E

E

D

A

E

P

Q

P

I

L

K

-

R

-

G

-

C

-

A

A

R

R

L

A

K

L

Q

D

E

E

C

L

S

S

Y

F

F

F

-

S

-

E

-

Q

E

E

W

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: Q117 (≠ R286), L121 (= L290), N134 (= N303), M135 (≠ L304), T136 (≠ A305), R137 (≠ K306), T150 (≠ Y319), F152 (= F321)

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
34% identity, 23% coverage: 10:95/368 of query aligns to 5:91/95 of 1ecmB

query
sites
1ecmB

V

L

R

R

E

E

S

K

I

I

D

S

T

A

I

L

D

D

N

E

S

K

I

L

L

L

E

A

L

L

K

A

K

E

R
|
R

I

R

G

E

L

L

A

A

K

V

E

E

I
x
V

G

G

R

K

L

A

K
|
K

S

L

E

L

D

S

N

H

R

R

S

P

K

V

W
x
R

D
|
D

P

I

L

D

R
|
R

E
|
E

R

R

E

D

I

L

Y

L

E

E

R

R

L

L

I

I

T

T

S

L

N

G

E

K

G

A

D

H

F

H

P

L

D

D

E

A

S

H

-

Y

L

I

R

T

S

R

I

L

F

F

H

Q

E

L

I

I

I
|
I

T

E

T

D

C
x
S

R

V

L

L

S

T

Q
|
Q

K

Q

K

A

A

L

V

L

V

Q

A

Q

Y

H

L

L

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R28), V30 (≠ I35), K34 (= K39), R42 (≠ W47), D43 (= D48), R46 (= R51), E47 (= E52), I76 (= I80), S79 (≠ C83), Q83 (= Q87)

Query Sequence

>WP_011189412.1 NCBI__GCF_000025945.1:WP_011189412.1
MKQDSNIPKVRESIDTIDNSILELLKKRIGLAKEIGRLKSEDNRSKWDPLREREIYERLI
TSNEGDFPDESLRSIFHEIITTCRLSQKKAVVAYLGPEATFSHLAGVKYFGHSTDYKPLE
TIDEVFAEVEKGRVQYGIVPVENSIEGAVFSTLDSFMKYKIKICGEMQLAISHNLVCKSG
NIEDIQTVASHNQPLAQCRDWLRKHLPNTPTLPVFSTGLAAKMAADNPNIGAIASSLAIS
TYDLQVVVKGIEDYEGNTTRFLIIGKESPGISGRDRTSLLIGLMDRPGALNEILSVLSEE
GINLAKIESRPIKGKQWKYLFFLDMIGHIEDEQIKRGCARLKQECSYFEWLGSYPQDENS
LEYSPANP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory