SitesBLAST

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
47% identity, 95% coverage: 4:157/162 of query aligns to 8:161/170 of A0QUX7

query
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A0QUX7

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K46 (≠ P42) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
47% identity, 96% coverage: 3:157/162 of query aligns to 76:228/477 of Q9FFF4

query
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Q9FFF4

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G88 (= G15) mutation to D: Resistance to valine inhibition.
T119 (= T48) mutation to I: In vat1-1; resistance to valine inhibition.

Sites not aligning to the query:

453 R→Q: In vat1-3; resistance to valine inhibition.

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 95% coverage: 4:157/162 of query aligns to 6:159/168 of P9WKJ3

query
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P9WKJ3

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K44 (≠ P42) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
45% identity, 96% coverage: 3:157/162 of query aligns to 4:156/159 of 6vz8F

query
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6vz8F

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binding valine: D13 (≠ N12), E14 (≠ Q13), G16 (= G15), M17 (≠ V16), I18 (≠ L17), N31 (= N30), I32 (= I31)

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
45% identity, 96% coverage: 3:157/162 of query aligns to 86:238/491 of Q93YZ7

query
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Q93YZ7

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D95 (≠ N12) binding L-valine
G98 (= G15) mutation to E: Increased feedback inhibition by valine/isoleucine.
M99 (≠ V16) binding L-valine
I100 (≠ L17) binding L-valine
F107 (= F24) mutation to A: Decreased feedback inhibition by leucine and valine/isoleucine.
Y112 (≠ F29) mutation to A: Increased feedback inhibition by leucine.
N113 (= N30) binding L-valine; mutation to A: Decreased feedback inhibition by valine/isoleucine.
I114 (= I31) binding L-valine

Sites not aligning to the query:

328 binding L-valine
331 G→D: Decreased feedback inhibition by leucine and valine/isoleucine.
332 binding L-valine
333 binding L-valine
340 F→A: Decreased feedback inhibition by valine/isoleucine.
345 Y→A: No effect.
346 binding L-valine; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
347 binding L-valine

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
41% identity, 97% coverage: 1:157/162 of query aligns to 1:157/159 of 6vz8G

query
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6vz8G

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binding valine: D12 (≠ N12), G15 (= G15), V16 (= V16), L17 (= L17), N30 (= N30), I31 (= I31), V36 (= V36)

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
32% identity, 96% coverage: 3:157/162 of query aligns to 4:195/197 of 6wo1B

query
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6wo1B

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binding valine: E14 (≠ Q13), G16 (= G15), L18 (= L17)

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 96% coverage: 3:158/162 of query aligns to 70:265/289 of O60086

query
sites
O60086

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R

V

L

T

S

G

G

L

I

F

L

S

A

G

A

R

R

G

G

F

F

N

N

I

I

E

D

S

S

L

L

C

V

V

V

A

C

E

A

T

T

L

E

D

V

P

E

K

N

I

L

S

S

C

R

L

M

T

T

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I

V

V

S

L

K

R

G

G

D

A

A

D

A

E

I

V

E

E

Q

Q

I

A

T

K

K

R

Q

Q

L

I

H

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K

D

L

I

I

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D

S

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V

I

W

K

A

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V

T

L

D

D

I

Y

S

T

E

G

H

T

E

S

Y

M

V

V

E

E

R

R

E

E

M

L

V

L

L

L

I

A

R

K

V

V

-

S

-

L

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P

-

D

-

H

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F

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Q

-

E

-

H

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F

-

E

-

R

-

S

-

E

-

K

-

V

-

A

-

E

-

S

-

T

-

N

-

A

-

K

-

A

-

K

-

S

-

D

-

G

-

E

-

G

-

V

-

M

-

N

-

A

N

N

A

A

E

A

V

L

H

Q

T

L

R

R

A

A

E

S

V

Q

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L

-

A

-

I

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N

R

Q

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K

E

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Y

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A

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A

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T

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P

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G

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G

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A

A

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M

A

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R

V

A

Sites not aligning to the query:

34 modified: Phosphoserine

6u9dL Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
30% identity, 96% coverage: 3:157/162 of query aligns to 37:240/255 of 6u9dL

query
sites
6u9dL

K

Q

H

H

T

V

I

L

S

N

V

C

L

L

L

V

Q

Q

N

N

Q

E

P

P

G

G

V

V

L

L

S

S

R

R

V

V

T

S

G

G

L

T

F

L

S

A

G

A

R

R

G

G

F

F

N

N

I

I

E

D

S

S

L

L

C

V

V

V

A

C

E

N

T

T

L

E

D

V

P

K

K

D

I

L

S

S

C

R

L

M

T

T

V

I

V

V

S

L

K

Q

G

G

D

Q

A

D

A

G

I

V

I

I

E

E

Q

Q

I

A

T

R

K

R

Q

Q

L

I

H

E

K

D

L

L

I

V

D

P

V

V

I

Y

K

A

V

V

T

L

D

D

I

Y

S

T

E

N

H

S

E

E

Y

I

V

I

E

K

R

R

E

E

M

L

V

V

L

M

I

A

R

R

V

I

N

S

-

L

-

G

-

T

-

E

-

Y

-

F

-

E

-

D

-

L

-

H

-

T

-

S

-

T

-

N

-

A

-

G

-

A

-

D

-

S

-

Q

-

E

-

L

-

V

A

A

E

E

V

I

H

R

T

E

R

K

-

Q

-

F

-

H

-

P

-

A

-

N

-

L

-

P

-

A

A

S

E

E

V

V

L

L

R

R

-

L

-

K

-

H

-

E

-

H

-

L

-

N

-

D

-

I

-

T

-

N

I

L

V

T

D

N

I
x
N

F
|
F

R

G

G

G

K

R

V

V

V
|
V

D
|
D

V
x
I

S

S

A

E

K

T

S

S

Y

C

A

I

I

V

E

E

V

L

T

S

G

A

N
x
K

T

P

S

T

K
x
R

I

I

H

S

A

A

V

F

T

L

D

K

L

L

L

V

R

E

P

P

L

F

G

G

I

V

K

L

E

E

I

C

V

A

R

R

T

S

G

G

T

M

I

M

A

A

M

L

A

P

R
|
R

binding adenosine-5'-triphosphate: N191 (≠ I108), F192 (= F109), V197 (= V114), D198 (= D115), I199 (≠ V116), K211 (≠ N128), R214 (≠ K131), R214 (≠ K131), R240 (= R157)

5yumA Crystallographic structures of ilvn.Val/ile complexes:conformational selectivity for feedback inhibition of ahass (see paper)
35% identity, 43% coverage: 6:74/162 of query aligns to 6:73/91 of 5yumA

query
sites
5yumA

I

L

S

E

V

L

L

T

L

V

Q

R

N
|
N

Q
x
H

P

P

G

G

V
|
V

L
x
M

S

T

R

H

V

V

T

C

G

G

L

L

F

F

S

A

G

R

R

R

G

A

F

F

N

N

I

V

E

E

S

G

L

I

C

L

V
x
C

A

L

E

P

T

I

L

Q

D

D

P

S

K

D

I

K

S

S

C

H

L

I

T

W

V

L

S

V

K

N

G

D

D

D

A

Q

A

R

I

-

I

L

E

E

Q

Q

I

M

T

I

K

S

Q

Q

L

I

H

D

K

K

L

L

I

E

D
|
D

V

V

I

V

K

K

V

V

binding cobalt (ii) ion: H13 (≠ Q13), D69 (= D70)
binding isoleucine: N12 (= N12), H13 (≠ Q13), V16 (= V16), M17 (≠ L17), C36 (≠ V36)

5yppE Crystal structure of ilvn.Val-1a (see paper)
35% identity, 43% coverage: 6:74/162 of query aligns to 6:73/91 of 5yppE

query
sites
5yppE

I

L

S

E

V

L

L

T

L

V

Q

R

N

N

Q
x
H

P

P

G

G

V

V

L
x
M

S

T

R

H

V

V

T

C

G

G

L

L

F

F

S

A

G

R

R

R

G

A

F

F

N
|
N

I
x
V

E

E

S

G

L

I

C

L

V
x
C

A

L

E

P

T

I

L

Q

D

D

P

S

K

D

I

K

S

S

C

H

L

I

T

W

V

L

S

V

K

N

G

D

D

D

A

Q

A

R

I

-

I

L

E

E

Q

Q

I

M

T

I

K

S

Q

Q

L

I

H

D

K

K

L

L

I

E

D

D

V

V

I

V

K

K

V

V

binding valine: H13 (≠ Q13), M17 (≠ L17), N30 (= N30), V31 (≠ I31), C36 (≠ V36)

Query Sequence

>WP_011190011.1 NCBI__GCF_000025945.1:WP_011190011.1
MMKHTISVLLQNQPGVLSRVTGLFSGRGFNIESLCVAETLDPKISCLTVVSKGDAAIIEQ
ITKQLHKLIDVIKVTDISEHEYVEREMVLIRVNAEVHTRAEVLRIVDIFRGKVVDVSAKS
YAIEVTGNTSKIHAVTDLLRPLGIKEIVRTGTIAMARVNKNK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory