SitesBLAST
Comparing WP_011190179.1 NCBI__GCF_000025945.1:WP_011190179.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3eleA Crystal structure of amino transferase (rer070207001803) from eubacterium rectale at 2.10 a resolution
40% identity, 99% coverage: 1:391/395 of query aligns to 2:395/398 of 3eleA
- active site: F134 (= F131), I208 (≠ F204), R210 (≠ S206), K248 (= K244)
- binding pyridoxal-5'-phosphate: G107 (= G105), A108 (= A106), F134 (= F131), N179 (= N175), N183 (= N179), D217 (= D213), Y220 (= Y216), S245 (= S241), S247 (= S243), K248 (= K244), R256 (= R252)
Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
32% identity, 91% coverage: 35:392/395 of query aligns to 32:385/385 of Q56232
- K234 (= K244) modified: N6-(pyridoxal phosphate)lysine
Sites not aligning to the query:
- 12 Important for prephenate aminotransferase activity; K→G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
32% identity, 89% coverage: 35:387/395 of query aligns to 32:380/382 of 1b5oA
- active site: W125 (≠ F131), D203 (= D213), I205 (≠ P215), K234 (= K244)
- binding pyridoxal-5'-phosphate: G99 (= G105), G100 (≠ A106), S101 (≠ A107), W125 (≠ F131), N175 (= N179), D203 (= D213), I205 (≠ P215), Y206 (= Y216), A233 (≠ S243), K234 (= K244), R242 (= R252)
1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
32% identity, 89% coverage: 35:387/395 of query aligns to 32:380/382 of 1gc4A
- binding aspartic acid: Y64 (= Y70), W125 (≠ F131), N175 (= N179), R261 (= R277), Y322 (= Y331), R361 (= R368)
- binding pyridoxal-5'-phosphate: G99 (= G105), G100 (≠ A106), S101 (≠ A107), W125 (≠ F131), N175 (= N179), D203 (= D213), Y206 (= Y216), R242 (= R252)
1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
32% identity, 89% coverage: 35:387/395 of query aligns to 32:380/382 of 1gc3A
- binding pyridoxal-5'-phosphate: G100 (≠ A106), S101 (≠ A107), W125 (≠ F131), N175 (= N179), D203 (= D213), Y206 (= Y216), K234 (= K244), R242 (= R252)
- binding tryptophan: T37 (≠ S40), A38 (≠ L41), G39 (= G42), E40 (≠ N43), Y322 (= Y331), R361 (= R368)
Sites not aligning to the query:
1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
32% identity, 89% coverage: 35:387/395 of query aligns to 32:380/382 of 1bkgA
- active site: W125 (≠ F131), D203 (= D213), I205 (≠ P215), K234 (= K244)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G105), G100 (≠ A106), K101 (≠ A107), W125 (≠ F131), N171 (= N175), N175 (= N179), D203 (= D213), I205 (≠ P215), Y206 (= Y216), K234 (= K244), R242 (= R252)
1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
32% identity, 89% coverage: 35:387/395 of query aligns to 32:380/382 of 1bjwA
1o4sB Crystal structure of aspartate aminotransferase (tm1255) from thermotoga maritima at 1.90 a resolution (see paper)
31% identity, 92% coverage: 17:379/395 of query aligns to 19:372/384 of 1o4sB
- active site: W131 (≠ F131), D209 (= D213), V211 (≠ P215), K242 (= K244)
- binding pyridoxal-5'-phosphate: G105 (= G105), A106 (= A106), K107 (≠ A107), W131 (≠ F131), N181 (= N179), D209 (= D213), V211 (≠ P215), Y212 (= Y216), S241 (= S243), K242 (= K244), R250 (= R252)
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
29% identity, 99% coverage: 2:391/395 of query aligns to 1:384/388 of 1gdeA
- active site: K232 (= K244)
- binding glutamic acid: F120 (= F131), N170 (= N179), R361 (= R368)
- binding pyridoxal-5'-phosphate: G94 (= G105), A95 (= A106), N96 (≠ A107), F120 (= F131), N166 (= N175), D198 (= D213), Y201 (= Y216), S231 (= S243), K232 (= K244), R240 (= R252)
1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
29% identity, 99% coverage: 2:391/395 of query aligns to 1:384/388 of 1gd9A
- active site: K232 (= K244)
- binding pyridoxal-5'-phosphate: G94 (= G105), A95 (= A106), N96 (≠ A107), F120 (= F131), N170 (= N179), D198 (= D213), V200 (≠ P215), Y201 (= Y216), S231 (= S243), K232 (= K244), R240 (= R252)
1j32A Aspartate aminotransferase from phormidium lapideum
30% identity, 89% coverage: 27:378/395 of query aligns to 25:373/388 of 1j32A
- active site: W124 (≠ F131), D202 (= D213), I204 (≠ P215), K237 (= K244)
- binding pyridoxal-5'-phosphate: G98 (= G105), G99 (≠ A106), K100 (≠ A107), W124 (≠ F131), N174 (= N179), D202 (= D213), I204 (≠ P215), Y205 (= Y216), K237 (= K244), R245 (= R252)
1xi9C Alanine aminotransferase from pyrococcus furiosus pfu-1397077-001
30% identity, 89% coverage: 35:387/395 of query aligns to 29:388/393 of 1xi9C
- active site: Y125 (≠ F131), D203 (= D213), I205 (≠ P215), K235 (= K244)
- binding pyridoxal-5'-phosphate: A99 (≠ G105), V100 (≠ A106), T101 (≠ A107), Y125 (≠ F131), N175 (= N179), D203 (= D213), I205 (≠ P215), Y206 (= Y216), S234 (= S243), K235 (= K244), R243 (= R252)
Q58097 (5-formylfuran-3-yl)methyl phosphate transaminase; 4-HFC-P:alanine aminotransferase; EC 2.6.1.108 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
27% identity, 92% coverage: 28:391/395 of query aligns to 23:370/370 of Q58097