SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_011317224.1 NCBI__GCF_000204075.1:WP_011317224.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

6cfwN Cryoem structure of a respiratory membrane-bound hydrogenase (see paper)
37% identity, 44% coverage: 75:133/134 of query aligns to 36:94/121 of 6cfwN

query
sites
6cfwN
G
 
G
E
 
K
I
 
L
V
 
V
V
 
Y
D
 
N
E
 
V
D
 
D
I
 
K
C
|
C
V
 
V
H
x
G
C
|
C
G
 
R
L
x
M
C
|
C
T
 
V
G
 
T
V
 
V
C
|
C
P
 
P
T
 
A
E
 
G
A
 
V
L
 
F
T
 
V
L
 
Y
H
 
L
P
 
P
E
 
E
T
 
I
Y
 
R
K
 
K
L
 
V
T
 
T
F
 
L
T
 
W
R
 
I
S
 
G
R
 
R
C
|
C
I
 
V
V
x
M
C
|
C
E
 
K
Q
 
Q
C
|
C
I
 
V
P
 
D
T
 
V
C
|
C
P
 
P
V
 
T
Q
 
A
A
 
A
I
 
L
S
 
Q
T
 
M
N
 
S

8a8oD Paps reductase from methanothermococcus thermolithotrophicus refined to 1.45 a (see paper)
39% identity, 40% coverage: 81:134/134 of query aligns to 6:59/102 of 8a8oD

query
sites
8a8oD
E
 
E
D
 
E
I
 
I
C
|
C
V
x
I
H
x
G
C
|
C
G
|
G
L
 
L
C
|
C
T
 
T
G
 
K
V
 
V
C
|
C
P
|
P
T
 
G
E
 
N
A
 
L
L
 
L
T
 
Y
L
 
Q
H
 
R
P
 
E
E
 
D
T
 
G
Y
 
K
K
x
S
L
 
E
T
 
I
F
 
M
T
 
D
R
 
K
S
 
R
R
 
D
C
|
C
I
x
W
V
x
D
C
|
C
E
x
A
Q
 
A
C
|
C
I
 
V
P
 
K
T
 
E
C
|
C
P
 
P
V
 
V
Q
 
N
A
 
A
I
|
I
S
 
E
T
 
M
N
 
Y
L
 
L

Sites not aligning to the query:

7p8nB Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
38% identity, 40% coverage: 78:130/134 of query aligns to 561:612/613 of 7p8nB

query
sites
7p8nB
V
 
V
V
 
I
D
 
N
E
 
P
D
 
D
I
 
I
C
|
C
V
 
K
H
 
G
C
|
C
G
|
G
L
 
L
C
|
C
T
 
A
G
 
R
V
 
S
C
|
C
P
 
P
T
 
Q
E
 
N
A
 
A
L
 
I
T
 
T
L
 
-
H
 
G
P
 
E
E
 
R
T
 
G
Y
 
K
K
 
P
L
x
Y
T
 
T
F
x
I
T
 
D
R
 
Q
S
 
E
R
 
K
C
|
C
I
 
V
V
x
K
C
|
C
E
x
G
Q
 
L
C
|
C
I
 
A
P
 
S
T
 
K
C
|
C
P
 
P
V
 
F
Q
 
K
A
 
A
I
 
I

Sites not aligning to the query:

5t61L Tungsten formylmethanofuran dehydrogenase subunit fwdF (see paper)
31% identity, 54% coverage: 59:130/134 of query aligns to 128:208/348 of 5t61L

query
sites
5t61L
A
 
A
I
|
I
E
 
T
W
 
I
M
 
T
R
 
R
S
 
E
R
 
L
H
 
P
I
 
E
N
 
R
V
 
K
S
 
D
L
 
L
T
 
V
L
 
T
G
 
G
E
 
E
I
|
I
V
 
E
V
 
I
D
 
D
E
 
K
D
 
D
I
 
T
C
|
C
V
 
I
H
 
Y
C
|
C
G
 
G
L
 
M
C
|
C
T
 
E
G
 
E
V
 
M
C
|
C
P
|
P
T
 
V
E
 
D
A
 
A
L
x
I
T
 
E
L
 
I
H
 
D
P
 
H
E
 
Q
T
 
T
-
 
P
-
 
S
-
 
S
-
 
A
-
 
S
-
 
P
-
 
V
-
 
V
-
 
A
Y
 
T
K
 
D
L
x
I
T
 
R
F
 
V
T
 
D
R
 
E
S
 
D
R
 
K
C
|
C
I
 
V
V
x
H
C
|
C
E
x
G
Q
 
I
C
|
C
I
 
K
P
 
R
T
 
I
C
|
C
P
|
P
V
 
V
Q
 
D
A
 
A
I
|
I

Sites not aligning to the query:

7q4vF Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
34% identity, 40% coverage: 79:131/134 of query aligns to 417:468/470 of 7q4vF

query
sites
7q4vF
V
x
I
D
 
N
E
 
A
D
 
D
I
 
T
C
|
C
V
 
K
H
x
G
C
|
C
G
|
G
L
x
I
C
|
C
T
 
A
G
 
K
V
 
K
C
|
C
P
|
P
T
 
A
E
 
D
A
 
A
L
x
I
T
 
S
L
 
G
H
 
E
P
 
K
E
 
K
T
 
K
Y
 
-
K
 
P
L
x
Y
T
 
N
F
 
I
T
 
D
R
 
T
S
 
S
R
 
K
C
|
C
I
 
I
V
x
K
C
|
C
E
x
G
Q
x
A
C
|
C
I
 
I
P
 
E
T
 
A
C
|
C
P
|
P
V
 
F
Q
 
G
A
 
S
I
|
I
S
 
S

Sites not aligning to the query:

8a5eB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
34% identity, 40% coverage: 79:131/134 of query aligns to 394:445/447 of 8a5eB

query
sites
8a5eB
V
 
I
D
 
N
E
 
A
D
 
D
I
 
T
C
|
C
V
 
K
H
x
G
C
|
C
G
|
G
L
 
I
C
|
C
T
 
A
G
 
K
V
 
K
C
|
C
P
|
P
T
 
A
E
 
D
A
 
A
L
x
I
T
 
S
L
 
G
H
 
E
P
 
K
E
 
K
T
 
K
Y
 
-
K
 
P
L
 
Y
T
 
N
F
 
I
T
 
D
R
 
T
S
 
S
R
 
K
C
|
C
I
 
I
V
x
K
C
|
C
E
x
G
Q
 
A
C
|
C
I
 
I
P
 
E
T
 
A
C
|
C
P
|
P
V
 
F
Q
 
G
A
 
S
I
|
I
S
 
S

Sites not aligning to the query:

7npaA Crystal structure of the coenzyme f420-dependent sulfite reductase from methanothermococcus thermolithotrophicus at 1.55-a resolution (see paper)
41% identity, 37% coverage: 78:126/134 of query aligns to 489:535/618 of 7npaA

query
sites
7npaA
V
 
V
V
 
V
D
 
N
E
 
E
D
 
E
I
 
N
C
|
C
V
x
N
H
x
G
C
|
C
G
|
G
L
 
R
C
|
C
T
 
A
G
 
E
V
 
V
C
|
C
P
x
K
T
x
I
E
 
E
A
|
A
L
x
I
T
 
D
L
 
I
H
 
R
P
 
G
E
 
E
T
 
T
Y
x
S
K
 
Y
L
 
T
T
 
N
F
 
Y
T
 
-
R
 
-
S
 
N
R
 
V
C
|
C
I
|
I
V
x
G
C
|
C
E
x
G
Q
 
K
C
|
C
I
 
I
P
 
K
T
 
A
C
|
C
P
 
P

Sites not aligning to the query:

7np8B Crystal structure of the coenzyme f420-dependent sulfite reductase from methanocaldococcus jannaschii at 2.3-a resolution (see paper)
37% identity, 38% coverage: 79:129/134 of query aligns to 490:538/620 of 7np8B

query
sites
7np8B
V
 
V
D
 
N
E
 
E
D
 
E
I
 
K
C
|
C
V
x
N
H
x
G
C
|
C
G
|
G
L
 
R
C
|
C
T
 
A
G
 
E
V
 
V
C
|
C
P
x
K
T
 
V
E
 
E
A
 
A
L
x
I
T
 
D
L
 
I
H
 
R
P
 
G
E
 
E
T
 
T
Y
x
S
K
 
Y
L
 
T
T
 
N
F
 
Y
T
 
-
R
 
-
S
 
N
R
 
V
C
|
C
I
x
V
V
x
G
C
|
C
E
x
G
Q
 
K
C
|
C
I
 
I
P
 
K
T
 
N
C
|
C
P
 
P
V
x
N
Q
 
E
A
|
A

Sites not aligning to the query:

8ugp1I NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (see paper)
40% identity, 37% coverage: 81:130/134 of query aligns to 27:81/126 of 8ugp1I

query
sites
8ugp1I
E
|
E
D
x
E
I
x
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
V
C
|
C
P
|
P
T
 
A
E
 
Q
A
 
A
L
x
I
T
|
T
L
 
I
H
 
E
P
x
A
E
|
E
T
x
P
Y
 
R
K
x
S
L
x
R
T
 
R
F
 
T
T
 
T
R
|
R
-
x
Y
-
x
D
-
 
I
-
 
D
-
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
 
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
|
P
V
|
V
Q
 
D
A
 
A
I
|
I

Sites not aligning to the query:

7arcI Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
34% identity, 37% coverage: 81:130/134 of query aligns to 97:154/199 of 7arcI

query
sites
7arcI
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
S
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
I
C
|
C
P
|
P
T
 
A
E
 
Q
A
 
A
L
x
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
E
-
 
R
-
 
E
-
 
D
-
 
G
-
 
S
-
 
R
-
 
K
T
 
T
Y
 
T
K
x
R
L
x
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
x
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
 
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

8ugh1I NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (see paper)
36% identity, 37% coverage: 81:130/134 of query aligns to 74:131/176 of 8ugh1I

query
sites
8ugh1I
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
|
L
C
|
C
T
 
E
G
 
A
V
 
V
C
|
C
P
|
P
T
 
A
E
 
Q
A
 
A
L
x
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
P
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
x
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
 
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
 
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

5gupH structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
36% identity, 37% coverage: 81:130/134 of query aligns to 74:131/176 of 5gupH

query
sites
5gupH
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
V
C
|
C
P
 
P
T
 
A
E
 
Q
A
 
A
L
 
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
P
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
x
R
L
 
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
x
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
|
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

7ak5I Cryo-em structure of respiratory complex i in the deactive state from mus musculus at 3.2 a (see paper)
34% identity, 37% coverage: 81:130/134 of query aligns to 76:133/178 of 7ak5I

query
sites
7ak5I
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
I
C
|
C
P
 
P
T
 
A
E
 
Q
A
 
A
L
 
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
P
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
 
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
 
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
 
P
V
 
V
Q
 
D
A
 
A
I
|
I

Sites not aligning to the query:

Q9VF27 NADH dehydrogenase (ubiquinone) 23 kDa subunit; EC 7.1.1.2 from Drosophila melanogaster (Fruit fly) (see paper)
34% identity, 37% coverage: 81:130/134 of query aligns to 115:172/217 of Q9VF27

query
sites
Q9VF27
E
 
E
D
 
E
I
 
R
C
 
C
V
 
I
H
 
A
C
 
C
G
 
K
L
 
L
C
 
C
T
 
E
G
 
A
V
 
I
C
 
C
P
 
P
T
 
A
E
 
Q
A
 
A
L
 
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
E
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
 
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
 
C
I
 
I
V
 
Y
C
 
C
E
 
G
Q
 
F
C
 
C
I
 
Q
P
 
E
T
 
A
C
 
C
P
 
P
V
 
V
Q
 
D
A
 
A
I
 
I

Sites not aligning to the query:

7zm7I Cryoem structure of mitochondrial complex i from chaetomium thermophilum (inhibited by ddm) (see paper)
36% identity, 37% coverage: 81:130/134 of query aligns to 83:140/185 of 7zm7I

query
sites
7zm7I
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
V
C
|
C
P
|
P
T
 
A
E
 
Q
A
 
A
L
x
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
E
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
x
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
 
G
Q
 
F
C
|
C
I
 
Q
P
 
E
T
 
S
C
|
C
P
 
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

8e73S8 qcr9 (see paper)
34% identity, 37% coverage: 81:130/134 of query aligns to 79:136/181 of 8e73S8

query
sites
8e73S8
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
 
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
I
C
|
C
P
 
P
T
 
A
E
 
Q
A
 
A
L
x
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
E
-
 
R
-
 
E
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
 
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
x
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
|
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

7b0nI 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
36% identity, 37% coverage: 81:130/134 of query aligns to 89:146/191 of 7b0nI

query
sites
7b0nI
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
I
C
|
C
P
 
P
T
 
A
E
 
L
A
 
A
L
 
I
T
 
T
L
 
I
H
 
D
P
 
A
E
 
E
-
 
E
-
 
R
-
 
I
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
K
L
 
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
 
Y
C
|
C
I
 
Q
P
 
E
T
 
S
C
|
C
P
 
P
V
 
V
Q
 
D
A
 
A
I
 
I

Sites not aligning to the query:

8b9zI Drosophila melanogaster complex i in the active state (dm1) (see paper)
34% identity, 37% coverage: 81:130/134 of query aligns to 84:141/186 of 8b9zI

query
sites
8b9zI
E
 
E
D
 
E
I
 
R
C
|
C
V
x
I
H
x
A
C
|
C
G
x
K
L
 
L
C
|
C
T
 
E
G
 
A
V
 
I
C
|
C
P
|
P
T
 
A
E
 
Q
A
 
A
L
 
I
T
 
T
L
 
I
H
 
E
P
 
A
E
 
E
-
 
E
-
 
R
-
 
A
-
 
D
-
 
G
-
 
S
-
 
R
-
 
R
T
 
T
Y
 
T
K
 
R
L
x
Y
T
 
D
F
 
I
T
 
D
R
 
M
S
 
T
R
 
K
C
|
C
I
|
I
V
x
Y
C
|
C
E
x
G
Q
x
F
C
|
C
I
 
Q
P
 
E
T
 
A
C
|
C
P
 
P
V
 
V
Q
 
D
A
|
A
I
|
I

Sites not aligning to the query:

Query Sequence

>WP_011317224.1 NCBI__GCF_000204075.1:WP_011317224.1
MKKRVTLTFPKRAIQMPVTYVLAKEFNVAANIIRAQVAPNQIGKLVVELSGDIDQLDAAI
EWMRSRHINVSLTLGEIVVDEDICVHCGLCTGVCPTEALTLHPETYKLTFTRSRCIVCEQ
CIPTCPVQAISTNL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory