SitesBLAST

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
28% identity, 69% coverage: 3:279/404 of query aligns to 5:286/384 of P75063

query
sites
P75063

D

D

F

V

T

L

I

I

V

V

G

G

I

V

V
x
I

G

G

L

C

S

A

T

T

G

A

M

Y

A

E

L

L

G

S

K

Q

R

Y

Y

-

P

-

Q

K

A

L

R

K

I

V

L

T

V

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L

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E
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E

K

K

E

H

S

H

Q

Y

W

L

A

A

F

Q

H

E

Q
x
T

T
x
S

G

H

N

A

N

N

S
|
S

G
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G

V
|
V

I

I

H

H

S

T

G

G

I

I

Y

D

Y

P

K

N

P

P

G

H

S

K

F

L

K

T

A

A

K

K

F

Y

C

N

R

I

D

L

G

G

R

K

D

K

S

L

M

W

V

L

K

N

-

T

F

Y

C

F

Q

K

E

R

Y

L

G

G

I

F

D

P

H

R

E

Q

V

K

C

I

G

R

K

T

V

L

I

I

V

V

A

A

T

F

N

N

G

E

Q

M

E

E

L

R

P

E

R

Q

L

L

E

E

N

V

L

L

Y

K

Q

Q

R

R

G

G

L

I

E

A

N

N

G

Q

I

I

-

N

-

L

-

E

E

D

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I

Q

Q

K

M

I

L

S

S

P

K

E

E

V

T

K

L

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K

I

L

E

E

P

P

H

Y

V

V

-

N

-

P

K

E

C

I

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V

A

A

G

G

I

L

R

K

V

I

F

E

S

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G

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A

V

I

N

D

-

P

-

V

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C

C

L

L

K

A

Y

L

V

A

E

A

L

-

I

-

Q

Q

Q

N

G

K

G

V

D

Q

L

I

R

C

L

T

N

N

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V

L

T

K

N

I

I

C

S

P

K

-

Q

-

V

S

D

G

G

K

T

N

Y

H

L

V

V

L

W

E

T

T

N

N

N

K

E

G

T

-

T

-

P

N

S

F

F

E

K

T

V

R

K

F

K

V

I

I

I

N

D

C

A

A

A

G

G

L

H

H

Y

S

A

D

D

R

Y

I

L

A

A

K

H

L

L

G

A

G

K

V

A

Q

D

P

D

S

F

A

E

K

Q

I

T

V

T

P

R

F

-

R

R

G

G

E

Q

Y

Y

Y

V

E

V

L

V

T

T

P

N

E

Q

K

G

R

E

Y

L

L

H

V

L

K

N

T

S

L

M

I

V

Y

F

P

M

V

V

P

P

N

T

P

I

E

H

F

G

P

K

F

-

L

-

G

G

V

V

H

I

F

V

T

S

R

P

M

M

I

L

D

D

G

G

S

N

V

F

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L

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V

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G

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P

N

T

A

A

V

L

L

D

S

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D

R

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E

E

G

A

Y

T

K

R

I14 (≠ V12) binding FAD
E33 (= E33) binding FAD
TS 42:43 (≠ QT 42:43) binding FAD
SGV 47:49 (= SGV 47:49) binding FAD
V177 (= V171) binding FAD

Sites not aligning to the query:

346:347 binding FAD
352 binding FAD

3sm8A Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with an (n5) flavin adduct (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 11:220/381 of 3sm8A

query
sites
3sm8A

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L
|
L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S
x
A

G

A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y
|
Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C

E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I
|
I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: I15 (≠ V7), G16 (= G8), G18 (= G10), I19 (= I11), A20 (≠ V12), L37 (= L32), E38 (= E33), R39 (≠ K34), H46 (= H41), S47 (≠ Q42), T48 (= T43), R50 (≠ N45), S51 (≠ N46), A52 (≠ S47), H54 (= H51), Y59 (= Y56), E176 (≠ K170), A177 (≠ V171), A205 (= A199), G206 (= G200), W208 (≠ H202), I212 (= I206)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 228, 246, 255, 309, 311, 336, 337, 338, 339, 340, 341, 342

3nyfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with imino-histidine (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 11:220/381 of 3nyfA

query
sites
3nyfA

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L
|
L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S
x
A

G

A

V

-

I

-

H
|
H

S

Y

G
x
T

I

V

Y

A

Y
|
Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C
x
E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

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P

P

H

V

V

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K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

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D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

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E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I
|
I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding flavin-adenine dinucleotide: I15 (≠ V7), G16 (= G8), G18 (= G10), I19 (= I11), A20 (≠ V12), L37 (= L32), E38 (= E33), R39 (≠ K34), H46 (= H41), S47 (≠ Q42), T48 (= T43), R50 (≠ N45), S51 (≠ N46), A52 (≠ S47), H54 (= H51), E176 (≠ K170), A177 (≠ V171), A205 (= A199), G206 (= G200), W208 (≠ H202), I212 (= I206)
binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: H54 (= H51), T56 (≠ G53), Y59 (= Y56), E93 (≠ C87)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 309, 311, 336, 337, 338, 339, 340, 341, 342
binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: 228, 246, 255, 311, 338

3nycA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 11:220/381 of 3nycA

query
sites
3nycA

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L
|
L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S

A

G
x
A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y
|
Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C
x
E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I
|
I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding flavin-adenine dinucleotide: I15 (≠ V7), G16 (= G8), G18 (= G10), I19 (= I11), A20 (≠ V12), L37 (= L32), E38 (= E33), R39 (≠ K34), H46 (= H41), S47 (≠ Q42), T48 (= T43), R50 (≠ N45), S51 (≠ N46), A53 (≠ G48), H54 (= H51), E176 (≠ K170), A177 (≠ V171), A205 (= A199), G206 (= G200), W208 (≠ H202), I212 (= I206)
binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: Y59 (= Y56), E93 (≠ C87)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 309, 311, 336, 337, 338, 339, 340, 341, 342
binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: 228, 246, 252, 255, 311, 338

7rdfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f co-crystallized in the presence of d-arginine (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 5:214/375 of 7rdfA

query
sites
7rdfA

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L
|
L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S
x
A

G

A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y
|
Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C
x
E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

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E

Q

V

M

Q

R

K

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I

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S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I

I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: I9 (≠ V7), G10 (= G8), G12 (= G10), I13 (= I11), A14 (≠ V12), L31 (= L32), E32 (= E33), R33 (≠ K34), H40 (= H41), S41 (≠ Q42), T42 (= T43), R44 (≠ N45), S45 (≠ N46), A46 (≠ S47), H48 (= H51), Y53 (= Y56), E87 (≠ C87), E170 (≠ K170), A171 (≠ V171), A199 (= A199), G200 (= G200), W202 (≠ H202)
binding 6-hydroxy-flavin-adenine dinucleotide: I9 (≠ V7), G10 (= G8), G12 (= G10), I13 (= I11), A14 (≠ V12), E32 (= E33), R33 (≠ K34), H40 (= H41), S41 (≠ Q42), T42 (= T43), S45 (≠ N46), A46 (≠ S47), H48 (= H51), E170 (≠ K170), A171 (≠ V171), A199 (= A199), G200 (= G200), W202 (≠ H202)

Sites not aligning to the query:

6pldA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with 6-oh-fad - green fraction (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 5:214/375 of 6pldA

query
sites
6pldA

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L
|
L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S
x
A

G

A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y

Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C

E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I

I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding 6-hydroxy-flavin-adenine dinucleotide: I9 (≠ V7), G10 (= G8), G12 (= G10), I13 (= I11), A14 (≠ V12), L31 (= L32), E32 (= E33), R33 (≠ K34), H40 (= H41), S41 (≠ Q42), T42 (= T43), R44 (≠ N45), S45 (≠ N46), A46 (≠ S47), H48 (= H51), E170 (≠ K170), A171 (≠ V171), A199 (= A199), G200 (= G200), W202 (≠ H202)

Sites not aligning to the query:

binding 6-hydroxy-flavin-adenine dinucleotide: 303, 305, 330, 331, 332, 333, 334, 335, 336

6p9dA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with fad - yellow fraction (see paper)
24% identity, 52% coverage: 3:214/404 of query aligns to 5:214/375 of 6p9dA

query
sites
6p9dA

D

D

F

Y

T

L

I

V

V
x
I

G
|
G

G

A

G
|
G

I
|
I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L

L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H
|
H

Q
x
S

T
|
T

G

G

N
x
R

N
x
S

S
x
A

G
x
A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y

Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C

E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K
x
E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A
|
A

G
|
G

L

A

H
x
W

S

C

D

D

R

A

I

I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

binding dihydroflavine-adenine dinucleotide: I9 (≠ V7), G10 (= G8), G12 (= G10), I13 (= I11), A14 (≠ V12), E32 (= E33), R33 (≠ K34), H40 (= H41), S41 (≠ Q42), T42 (= T43), R44 (≠ N45), S45 (≠ N46), A46 (≠ S47), A47 (≠ G48), H48 (= H51), E170 (≠ K170), A171 (≠ V171), A199 (= A199), G200 (= G200), W202 (≠ H202)

Sites not aligning to the query:

binding dihydroflavine-adenine dinucleotide: 303, 305, 330, 331, 332, 333, 334, 335, 336

Q9HXE3 FAD-dependent catabolic D-arginine dehydrogenase DauA; D-arginine dehydrogenase; DADH; D-arginine utilization protein A; Dau; EC 1.4.99.6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
24% identity, 52% coverage: 3:214/404 of query aligns to 5:214/375 of Q9HXE3

query
sites
Q9HXE3

D

D

F

Y

T

L

I

V

V

I

G

G

G

A

G

G

I

I

V
x
A

G

G

L

A

S

S

T

T

G

G

M

Y

A

W

L

L

G

S

K

A

R

-

Y

-

P

-

Q

H

A

G

R

R

I

V

L

V

V

V

L

L

E
|
E

K
x
R

E

E

S

A

Q

Q

W

P

A

G

F

Y

H

H

Q
x
S

T
|
T

G
|
G

N
x
R

N
x
S

S
x
A

G
x
A

V

-

I

-

H
|
H

S

Y

G

T

I

V

Y

A

Y

Y

K

G

P

T

G

P

S

Q

F

V

K

R

A

A

K

L

F

T

C

A

R

A

D

S

G

R

R

A

-

F

-

D

D

N

S

P

M

P

V

A

K

G

F

F

C

C

Q

E

E

H

Y

P

G

L

I

L

D

S

H

P

E

R

V

P

C
x
E

G

M

K

V

V

V

I

D

V

F

A

S

T

D

N

D

G

P

Q

E

E

E

L

L

P

R

R

R

L

-

E

-

N

-

L

Q

Y

Y

Q

E

R

S

G

G

L

K

E

A

N

L

G

V

I

P

E

Q

V

M

Q

R

K

L

I

L

S

D

P

A

E

E

E

Q

V

A

K

C

E

S

I

I

E

V

P

P

H

V

V

L

K

R

-

R

-

D

C

K

V

V

A

F

G

G

I

A

R

T

V

Y

F

D

S

P

T

T

G

G

I

A

-

D

V

I

N

D

Y

T

K

D

Q

A

V

L

C

H

L

Q

K

G

Y

Y

V

L

E

R

L

G

I

I

Q

R

Q

N

G

Q

G

G

D

Q

L

V

R

L

L

C

N

N

T

H

K

E

V
x
A

L

L

K

E

I

I

C

R

P

R

S

V

G

D

K

G

N

A

H

W

V

E

L

V

E

R

T

C

N

D

K

A

G

G

N

S

F

Y

E

R

T

A

R

A

F

V

V

L

I

V

N

N

C

A

A

A

G

G

L

A

H

W

S

C

D

D

R

A

I

I

A

A

K

G

L

L

G

A

G

G

V

V

Q

R

P

P

A14 (≠ V12) binding FAD
ER 32:33 (≠ EK 33:34) binding FAD
41:48 (vs. 42:51, 30% identical) binding FAD
E87 (≠ C87) Important for specificity toward positively charged substrates
A171 (≠ V171) binding FAD

Sites not aligning to the query:

331:336 binding FAD

Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
24% identity, 56% coverage: 6:230/404 of query aligns to 9:232/830 of Q9AGP8

query
sites
Q9AGP8

I

I

V

I

G

G

G

A

G

G

I
|
I

V
|
V

G

G

L

T

S

N

T

L

G

A

M

D

A

E

L

L

G

V

K

T

R

R

Y

-

P

G

Q

W

A

N

R

N

I

I

L

T

V

V

L

L

E
x
D

K

-

E

-

S

-

Q
|
Q

W

G

A

P

F

L

H

N

Q

M

T

P

G

G

N

G

N
x
S

S
x
T

G
x
S

V

-

I

-

H
|
H

S

A

-

P

G

G

I
x
L

Y

V

Y

F

K

Q

-

T

-

N

P

P

G

S

S

K

F

T

K

M

A

A

K

S

F

F

C

A

R

K

D

Y

G

T

R

V

D

E

S

K

M

L

V

L

K

S

F

L

C

T

Q

E

E

D

Y

G

G

V

I

S

D

C

H

F

E

N

V

Q

C

V

G

G

K

G

V

L

I

E

V

V

A

A

T

T

N

T

G

E

Q

T

E

R

L

L

P

A

R

D

L

L

E

K

N

R

L

K

Y

L

Q

G

R

Y

G

A

L

A

E

A

N

W

G

G

I

I

E

E

V

G

Q

R

K

L

I

L

S

S

P

P

E

A

E

E

V

C

K

Q

E

E

I

L

E

Y

P

P

H

L

V

L

-

D

-

G

-

E

K

N

C

I

V

L

A

G

G

G

I

L

R

H

V

V

F

P

S

S

T

D

G

G

I

L

V

A

N

S

Y

A

K

A

Q

R

V

A

C

V

L

Q

K

L

Y

L

V

I

E

K

L

R

I

T

Q

E

Q

S

Q

A

G

G

G

V

D

T

L

Y

R

R

L

G

N

S

T

T

K

T

V
|
V

L

T

K

G

I

I

C

E

P

Q

S

S

G

G

K

G

N

R

H

V

V

T

-

G

L

V

E

Q

T

T

N

A

K

D

G

G

N

V

F

I

E

P

T

A

R

D

F

I

V

V

I

V

N

S

C

C

A

A

G

G

L

F

H

W

S

G

D

A

R

K

I

I

A

G

K

A

L

M

G

I

G

G

V

M

Q

-

P

-

S

A

A

V

K

P

I

L

V

L

P

P

F

L

R

A

G
x
H

E

Q

Y

Y

Y

V

E

K

L

T

T

T

P

P

IV 14:15 (= IV 11:12) binding FAD
D---Q 35:36 (≠ EKESQ 33:37) binding FAD
STS--H 45:48 (≠ NSGVIH 46:51) binding FAD
L52 (≠ I54) binding FAD
V174 (= V171) binding FAD
H225 (≠ G223) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.

Sites not aligning to the query:

259 Important for catalytic activity; binding FAD; Y→F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
360:363 binding FAD
539 binding (6S)-5,6,7,8-tetrahydrofolate
552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.

3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
24% identity, 56% coverage: 6:230/404 of query aligns to 6:229/827 of 3gsiA

query
sites
3gsiA

I

I

V

I

G

G

G

A

G
|
G

I
|
I

V
|
V

G

G

L

T

S

N

T

L

G

A

M

D

A

E

L

L

G

V

K

T

R

R

Y

-

P

G

Q

W

A

N

R

N

I

I

L

T

V

V

L

L

E
x
D

K

-

E

-

S

-

Q
|
Q

W

G

A

P

F

L

H

N

Q

M

T

P

G

G

N
x
G

N
x
S

S
x
T

G

S

V

-

I

-

H
|
H

S

A

-
x
P

G

G

I
x
L

Y

V

Y

F

K

Q

-

T

-

N

P

P

G

S

S

K

F

T

K

M

A

A

K

S

F

F

C

A

R

K

D

Y

G

T

R

V

D

E

S

K

M

L

V

L

K

S

F

L

C

T

Q

E

E

D

Y

G

G

V

I

S

D

C

H

F

E

N

V

Q

C

V

G

G

K

G

V

L

I

E

V

V

A

A

T

T

N

T

G

E

Q

T

E

R

L

L

P

A

R

D

L

L

E

K

N

R

L

K

Y

L

Q

G

R

Y

G

A

L

A

E

A

N

W

G

G

I

I

E

E

V

G

Q

R

K

L

I

L

S

S

P

P

E

A

E

E

V

C

K

Q

E

E

I

L

E

Y

P

P

H

L

V

L

-

D

-

G

-

E

K

N

C

I

V

L

A

G

G

G

I

L

R

H

V

V

F

P

S

S

T

D

G

G

I

L

V

A

N

S

Y

A

K

A

Q

R

V

A

C

V

L

Q

K

L

Y

L

V

I

E

K

L

R

I

T

Q

E

Q

S

Q

A

G

G

G

V

D

T

L

Y

R

R

L

G

N

S

T

T

K
x
T

V
|
V

L

T

K

G

I

I

C

E

P

Q

S

S

G

G

K

G

N

R

H

V

V

T

-

G

L

V

E

Q

T

T

N

A

K

D

G

G

N

V

F

I

E

P

T

A

R

D

F

I

V

V

I

V

N

S

C

C

A
|
A

G
|
G

L

F

H
x
W

S

G

D

A

R

K

I

I

A

G

K

A

L

M

G

I

G

G

V

M

Q

-

P

-

S

A

A

V

K

P

I

L

V

L

P

P

F

L

R

A

G
x
H

E

Q

Y

Y

Y

V

E

K

L

T

T

T

P

P

active site: H222 (≠ G223)
binding flavin-adenine dinucleotide: G10 (= G10), I11 (= I11), V12 (= V12), D32 (≠ E33), Q33 (= Q37), G41 (≠ N45), S42 (≠ N46), T43 (≠ S47), H45 (= H51), P47 (vs. gap), L49 (≠ I54), T170 (≠ K170), V171 (= V171), A200 (= A199), G201 (= G200), W203 (≠ H202), H222 (≠ G223)

Sites not aligning to the query:

active site: 256, 549
binding flavin-adenine dinucleotide: 256, 330, 331, 332, 357, 358, 359, 360
binding magnesium ion: 254, 409
binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696

1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
24% identity, 56% coverage: 6:230/404 of query aligns to 7:230/828 of 1pj6A

query
sites
1pj6A

I

I

V

I

G
|
G

G

A

G
|
G

I
|
I

V
|
V

G

G

L

T

S

N

T

L

G

A

M

D

A

E

L

L

G

V

K

T

R

R

Y

-

P

G

Q

W

A

N

R

N

I

I

L

T

V

V

L

L

E
x
D

K

-

E

-

S

-

Q
|
Q

W

G

A

P

F

L

H

N

Q

M

T

P

G

G

N
x
G

N
x
S

S
x
T

G

S

V

-

I

-

H
|
H

S

A

-
x
P

G

G

I
x
L

Y

V

Y

F

K

Q

-

T

-

N

P

P

G

S

S

K

F

T

K

M

A

A

K

S

F

F

C

A

R

K

D

Y

G

T

R

V

D

E

S

K

M

L

V

L

K

S

F

L

C

T

Q

E

E

D

Y

G

G

V

I

S

D

C

H

F

E

N

V

Q

C

V

G

G

K

G

V

L

I

E

V

V

A

A

T

T

N

T

G

E

Q

T

E

R

L

L

P

A

R

D

L

L

E

K

N

R

L

K

Y

L

Q

G

R

Y

G

A

L

A

E

A

N

W

G

G

I

I

E

E

V

G

Q

R

K

L

I

L

S

S

P

P

E

A

E

E

V

C

K

Q

E

E

I

L

E

Y

P

P

H

L

V

L

-

D

-

G

-

E

K

N

C

I

V

L

A

G

G

G

I

L

R

H

V

V

F

P

S

S

T

D

G

G

I

L

V

A

N

S

Y

A

K

A

Q

R

V

A

C

V

L

Q

K

L

Y

L

V

I

E

K

L

R

I

T

Q

E

Q

S

Q

A

G

G

G

V

D

T

L

Y

R

R

L

G

N

S

T

T

K

T

V
|
V

L

T

K

G

I

I

C

E

P

Q

S

S

G

G

K

G

N

R

H

V

V

T

-

G

L

V

E

Q

T

T

N

A

K

D

G

G

N

V

F

I

E

P

T

A

R

D

F

I

V

V

I

V

N

S

C

C

A
|
A

G
|
G

L

F

H
x
W

S

G

D

A

R

K

I

I

A

G

K

A

L

M

G

I

G

G

V

M

Q

-

P

-

S

A

A

V

K

P

I

L

V

L

P

P

F

L

R

A

G
x
H

E

Q

Y

Y

Y

V

E

K

L

T

T

T

P

P

active site: H223 (≠ G223)
binding flavin-adenine dinucleotide: G9 (= G8), G11 (= G10), I12 (= I11), V13 (= V12), D33 (≠ E33), Q34 (= Q37), G42 (≠ N45), S43 (≠ N46), T44 (≠ S47), H46 (= H51), P48 (vs. gap), L50 (≠ I54), V172 (= V171), A201 (= A199), G202 (= G200), W204 (≠ H202), H223 (≠ G223)

Sites not aligning to the query:

active site: 257, 550
binding flavin-adenine dinucleotide: 257, 331, 332, 358, 359, 360, 361

1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
24% identity, 56% coverage: 6:230/404 of query aligns to 6:229/827 of 1pj7A

query
sites
1pj7A

I

I

V

I

G
|
G

G

A

G
|
G

I
|
I

V
|
V

G

G

L

T

S

N

T

L

G

A

M

D

A

E

L

L

G

V

K

T

R

R

Y

-

P

G

Q

W

A

N

R

N

I

I

L

T

V

V

L

L

E
x
D

K

-

E

-

S

-

Q
|
Q

W

G

A

P

F

L

H

N

Q

M

T

P

G

G

N
x
G

N
x
S

S
x
T

G

S

V

-

I

-

H
|
H

S

A

-
x
P

G

G

I
x
L

Y

V

Y

F

K

Q

-

T

-

N

P

P

G

S

S

K

F

T

K

M

A

A

K

S

F

F

C

A

R

K

D

Y

G

T

R

V

D

E

S

K

M

L

V

L

K

S

F

L

C

T

Q

E

E

D

Y

G

G

V

I

S

D

C

H

F

E

N

V

Q

C

V

G

G

K

G

V

L

I

E

V

V

A

A

T

T

N

T

G

E

Q

T

E

R

L

L

P

A

R

D

L

L

E

K

N

R

L

K

Y

L

Q

G

R

Y

G

A

L

A

E

A

N

W

G

G

I

I

E

E

V

G

Q

R

K

L

I

L

S

S

P

P

E

A

E

E

V

C

K

Q

E

E

I

L

E

Y

P

P

H

L

V

L

-

D

-

G

-

E

K

N

C

I

V

L

A

G

G

G

I

L

R

H

V

V

F

P

S

S

T

D

G

G

I

L

V

A

N

S

Y

A

K

A

Q

R

V

A

C

V

L

Q

K

L

Y

L

V

I

E

K

L

R

I

T

Q

E

Q

S

Q

A

G

G

G

V

D

T

L

Y

R

R

L

G

N

S

T

T

K
x
T

V
|
V

L

T

K

G

I

I

C

E

P

Q

S

S

G

G

K

G

N

R

H

V

V

T

-

G

L

V

E

Q

T

T

N

A

K

D

G

G

N

V

F

I