SitesBLAST

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
28% identity, 30% coverage: 149:258/369 of query aligns to 121:225/225 of Q8U122

query
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Q8U122

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D121 (= D149) binding ; binding
G179 (= G211) binding
S182 (≠ G214) binding

Sites not aligning to the query:

6 binding
44 binding
66 binding
114:120 binding
120 binding

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
28% identity, 30% coverage: 149:258/369 of query aligns to 122:226/226 of 2bmuB

query
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2bmuB

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binding phosphoaminophosphonic acid-adenylate ester: T141 (= T168), N142 (≠ D169), V146 (≠ L173), Y147 (= Y174), A149 (= A176), D150 (= D177), P151 (= P178), S182 (≠ G213), S183 (≠ G214), V184 (≠ M215)
binding magnesium ion: D122 (= D149), D122 (= D149), S183 (≠ G214), V184 (≠ M215)
binding uridine-5'-monophosphate: A179 (≠ W210)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
binding magnesium ion: 7
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120, 121

2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
27% identity, 30% coverage: 149:258/369 of query aligns to 123:219/219 of 2ji5A

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2ji5A

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Sites not aligning to the query:

binding uridine 5'-triphosphate: 68, 71, 72, 115, 116, 117, 120, 121, 122

7n9dA I74a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
26% identity, 36% coverage: 120:252/369 of query aligns to 121:248/253 of 7n9dA

query
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7n9dA

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binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: G135 (≠ N135), D136 (= D136), V137 (≠ T137), I147 (vs. gap), S149 (≠ D147), G150 (≠ N148), T208 (= T212)
binding adenosine-5'-diphosphate: S170 (≠ T168), D171 (= D169), I172 (≠ V170), Y176 (= Y174), A178 (= A176), D179 (= D177), D201 (= D198), G210 (= G214), V211 (≠ M215), K214 (= K218)

Sites not aligning to the query:

binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: 45, 46, 50, 51, 75, 79
binding adenosine-5'-diphosphate: 8, 9, 10, 15

Query Sequence

>WP_011320502.1 NCBI__GCF_000204075.1:WP_011320502.1
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLG
LTERPKAIALKQAVAAVGQGRLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTF
RELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEADWLFLLTDVDRLYSADPRS
VPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPA
GVKTVAGEFEPQDAVQLLDPQGNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETV
IHRDNLVLT

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory