SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011320646.1 NCBI__GCF_000204075.1:WP_011320646.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
48% identity, 64% coverage: 35:195/250 of query aligns to 84:244/250 of 4hzdA

query
sites
4hzdA

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binding coenzyme a: H158 (= H109), G203 (= G154), A204 (≠ S155), A221 (≠ G172), A222 (= A173), T235 (= T186), A237 (≠ V188), I244 (= I195)
binding magnesium ion: S224 (= S175), A237 (≠ V188), G238 (= G189)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
41% identity, 75% coverage: 1:187/250 of query aligns to 72:258/280 of 7bw9A

query
sites
7bw9A

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binding cysteine: D114 (= D43), P115 (= P44), D179 (= D108), H180 (= H109), A206 (≠ G135), R214 (= R143), H215 (= H144)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
44% identity, 67% coverage: 34:201/250 of query aligns to 84:251/261 of 6wyeA

query
sites
6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D43), P94 (= P44), D158 (= D108), H159 (= H109), G185 (= G135), R193 (= R143), H194 (= H144)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
43% identity, 71% coverage: 30:207/250 of query aligns to 75:251/258 of 4h7oA

query
sites
4h7oA

E

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binding arginine: Y127 (≠ L82), Q131 (≠ L86)
binding cysteine: D88 (= D43), P89 (= P44), D153 (= D108), H154 (= H109), G180 (= G135), R188 (= R143), H189 (= H144)

Sites not aligning to the query:

binding arginine: 51, 53

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
43% identity, 69% coverage: 35:207/250 of query aligns to 87:258/272 of 3gvdI

query
sites
3gvdI

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binding cysteine: D95 (= D43), P96 (= P44), D160 (= D108), G186 (= G134), G187 (= G135), R195 (= R143)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
45% identity, 64% coverage: 34:193/250 of query aligns to 82:241/243 of 7ra4A

query
sites
7ra4A

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binding serine: D91 (= D43), P92 (= P44), D156 (= D108), H157 (= H109), R191 (= R143), H192 (= H144)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
42% identity, 68% coverage: 35:203/250 of query aligns to 80:252/258 of 8i04A

query
sites
8i04A

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H

P

D

H

C

T

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

-

G

-

K

-

P

-

G

T

S

H

D

K

K

P

P

K

S

T

M

D

D

L

M

binding serine: D88 (= D43), P89 (= P44), D153 (= D108), H154 (= H109), G180 (= G135), R188 (= R143), H189 (= H144)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
48% identity, 62% coverage: 32:187/250 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

L

L

L

K

S

T

D

D

F

L

L

I

I

A

I

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

W

L

L

S

E

L

V

I

I

I

F

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

I

V

C

I

L

V

H

Y

R

R

F

I

A

A

H

H

W

V

L

L

H

Y

R

E

R

C

G

G

V

E

V

R

F

Y

F

Y

P

C

R

R

L

E

I

M

S

M

H

E

L

S

A

V

R

H

F

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

V

S

I

I

G

K

K

G

G

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

I

I

V

I

G

G

D

E

Y

W

A

C

L

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

S

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

V

V

G

G

D

D

H

Y

V

V

V

T

V

I

G

G

S

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

Q

I

I

V

G

G

D

S

R

H

V

V

R

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

R

R

D

D

V

V

P

D

H

S

D

N

C

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R143), H225 (= H144)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
48% identity, 62% coverage: 32:187/250 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

L

L

L

K

S

T

D

D

F

L

L

I

I

A

I

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

W

L

L

S

E

L

V

I

I

I

F

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

I

V

C

I

L

V

H

Y

R

R

F

I

A

A

H

H

W

V

L

L

H

Y

R

E

R

C

G

G

V

E

V

R

F

Y

F

Y

P

C

R

R

L

E

I

M

S

M

H

E

L

S

A

V

R

H

F

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

V

S

I

I

G

K

K

G

G

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

I

I

V

I

G

G

D

E

Y

W

A

C

L

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

S

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

V

V

G

G

D

D

H

Y

V

V

V

T

V

I

G

G

S

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

Q

I

I

V

G

G

D

S

R

H

V

V

R

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

R

R

D

D

V

V

P

D

H

S

D

N

C

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R143), H223 (= H144)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
41% identity, 68% coverage: 35:204/250 of query aligns to 80:253/257 of 1ssqD

query
sites
1ssqD

D

D

F

I

L

Q

I

A

I

V

F

R

E

H

R

R

D
|
D

P

P

A

A

A

V

R

E

N

L

W

W

L

S

E

T

V

P

I

L

F

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

I

C

Q

L

S

H

Y

R

R

F

I

A

T

H

H

W

Y

L

L

H

W

R

N

R

Q

G

N

V

R

V

K

F

S

F

L

P

A

R

L

L

Y

I

L

S

Q

H

N

L

Q

A

I

R

S

F

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

K

H

G

G

V

I

F

M

I

F

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

I

V

V

I

G

E

D

N

Y

D

A

V

L

S

I

I

Y

L

Q

Q

G

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

E

S

S

G

G

K

D

R

R

H

H

P

P

T

K

V

V

G

R

D

E

H

G

V

V

V

M

V

I

G

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

Q

E

I

V

G

G

D

K

R

Y

V

A

R

K

I

I

G

G

A

A

G

N

S

S

V

V

V

V

L

L

R

N

D

P

V

V

P

P

H

E

D

Y

C

A

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

T

S

H

Q

-

D

-

K

-

A

-

A

K

K

P

P

K

A

T

F

D

D

L

M

S

N

binding cysteine: D88 (= D43), D153 (= D108), H154 (= H109)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
43% identity, 64% coverage: 35:195/250 of query aligns to 83:243/246 of 8i09A

query
sites
8i09A

D

D

F

I

L

Q

I

A

I

V

F

R

E

T

R

R

D
|
D

P

P

A

A

A

V

R

D

N

K

W

Y

L

S

E

T

V

P

I

L

F

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

L

C

Q

L

A

H

Y

R

R

F

I

A

G

H

H

W

W

L

L

H

W

R

N

R

K

G

G

V

R

V

R

F

A

F

L

P

A

R

I

L

F

I

L

S

Q

H

N

L

Q

A

V

R

S

F

V

L

S

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

K

R

G

G

V

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G
|
G

E

E

T

T

A

A

I

V

V

I

G

E

D

D

Y

D

A

V

L

S

I

I

Y

L

Q

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

G

K

K

E

T

S

S

G

G

K

D

R
|
R

H
|
H

P

P

T

K

V

I

G

R

D

E

H

G

V

V

V

M

V

I

G
|
G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

Q

E

I

V

G

G

D

R

R

G

V

A

R
x
K

I
|
I

G
|
G

A

A

G

G

S

S

V

V

V

V

L
|
L

R

Q

D

P

V

V

P

P

H

P

D

H

C

T

T

T

V

A

V
x
A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

binding cysteine: D91 (= D43), D156 (= D108), H157 (= H109), G164 (= G116), R191 (= R143), H192 (= H144)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G135), T184 (= T136), G202 (= G154), K218 (≠ R170), I219 (= I171), G220 (= G172), L226 (= L178), A236 (≠ V188)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
43% identity, 64% coverage: 35:195/250 of query aligns to 84:244/244 of 8i06A

query
sites
8i06A

D

D

F

I

L

Q

I

A

I

V

F

R

E

T

R

R

D

D

P
|
P

A

A

A

V

R

D

N

K

W

Y

L

S

E

T

V

P

I

L

F

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

L

C

Q

L

A

H

Y

R

R

F

I

A

G

H

H

W

W

L

L

H

W

R

N

R

K

G

G

V

R

V

R

F

A

F

L

P

A

R

I

L

F

I

L

S

Q

H

N

L

Q

A

V

R

S

F

V

L

S

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

K

R

G

G

V

I

F

M

I

L

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

I

V

V

I

G

E

D

D

Y

D

A

V

L

S

I

I

Y
x
L

Q
|
Q

G

S

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

T

S

S

G

G

K

D

R

R

H

H

P

P

T

K

V

I

G

R

D

E

H

G

V

V

V

M

V

I

G
|
G

S
x
A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

Q

E

I

V

G

G

D

R

R

G

V

A

R

K

I

I

G
|
G

A
|
A

G

G

S

S

V

V

V

V

L

L

R

Q

D

P

V

V

P

P

H

P

D

H

C

T

T
|
T

V

A

V
x
A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

binding coenzyme a: H158 (= H109), L177 (≠ Y128), Q178 (= Q129), G203 (= G154), A204 (≠ S155), G221 (= G172), A222 (= A173), T235 (= T186), A237 (≠ V188)
binding cysteine: P93 (= P44), H158 (= H109)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
40% identity, 68% coverage: 35:203/250 of query aligns to 84:256/262 of 1t3dA

query
sites
1t3dA

D

D

F

I

L

Q

I

A

I

V

F

R

E

T

R

R

D
|
D

P
|
P

A

A

A

V

R

D

N

K

W

Y

L

S

E

T

V

P

I

L

F

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

L

C

Q

L

A

H

Y

R

R

F

I

A

G

H

H

W

W

L

L

H

W

R

N

R

Q

G

G

V

R

V

R

F

A

F

L

P

A

R

I

L

F

I

L

S

Q

H

N

L

Q

A

V

R

S

F

V

L

T

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

K

R

G

G

V

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

I

V

V

I

G

E

D

N

Y

D

A

V

L

S

I

I

Y

L

Q

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

S

S

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

V

I

G

R

D

E

H

G

V

V

V

M

V

I

G

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

Q

E

I

V

G

G

D

R

R

G

V

A

R

K

I

I

G

G

A

A

G

G

S

S

V

V

V

V

L

L

R

Q

D

P

V

V

P

P

H

P

D

H

C

T

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

-

G

-

K

-

P

-

D

T

S

H

D

K

K

P

P

K

S

T

M

D

D

L

M

binding cysteine: D92 (= D43), P93 (= P44), D157 (= D108), G184 (= G135), R192 (= R143), H193 (= H144)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
41% identity, 72% coverage: 17:195/250 of query aligns to 55:243/243 of 4n69A

query
sites
4n69A

T

S

S

T

L

L

V

L

K

S

N

T

L

L

L

L

S

Y

G

D

V

L

F

F

L

L

E

N

P

A

L

F

-

S

-

S

-

D

-

P

-

S

-

L

-

R

-

S

-

A

-

A

L

V

S

A

D

D

F

L

L

R

I

A

I

A

F

R

E

E

R

R

D

D

P

P

A

A

A

C

R

V

N

S

W

Y

L

S

E

H

V

C

I

L

F

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

I

C

C

Q

L

A

H

H

R

R

F

V

A

A

H

H

W

L

L

L

H

W

R

R

R

Q

G

S

V

R

V

R

F

P

F

L

P

A

R

L

L

A

I

L

S

H

H

S

L

R

A

I

R

A

F

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

I

G

G

K

K

G

G

V

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

I

V

V

I

G

G

D

N

Y

N

A

V

L

S

I

I

Y

L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

S

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

V

I

G

G

D

D

H

G

V

V

V

L

V

I

G

G

S

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

Q

K

I

I

G

G

D

E

R

G

V

A

R

K

I

V

G

G

A

A

G

G

S

S

V

V

V

V

L

L

R

I

D

D

V

V

P

P

H

P

D

R

C

T

T

T

V

A

V

V

G

G

I

N

P

P

G

A

R

R

I

L

I

V

binding serine: G183 (= G135), R191 (= R143), H192 (= H144)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
41% identity, 71% coverage: 19:195/250 of query aligns to 44:233/233 of 4n6bA

query
sites
4n6bA

L

L

V

G

K

N

N

K

L

L

L

C

S

S

G

S

V

T

F

L

L

L

E

S

P

T

L

L

L

L

S

Y

D

D

-

L

F

F

L

L

I

N

I

A

F

F

E

S

R

S

D

D

P

P

A

S

A

L

R

R

N

S

-

A

-

A

-

V

-

A

-

D

-

L

-

R

-

A

-

A

-

R

-

E

-

R

-

D

-

V

-

S

W

Y

L

S

E

H

V

C

I

L

F

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

I

C

C

Q

L

A

H

H

R

R

F

V

A

A

H

H

W

L

L

L

H

W

R

R

R

Q

G

S

V

R

V

R

F

P

F

L

P

A

R

L

L

A

I

L

S

H

H

S

L

R

A

I

R

A

F

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

I

G

G

K

K

G

G

V

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

I

V

V

I

G

G

D

N

Y

N

A

V

L

S

I

I

Y
x
L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

S

-

G

G

K

D

R

R

H

H

P

P

T

K

V

I

G

G

D

D

H

G

V

V

V

L

V

I

G

G

S

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

Q

K

I

I

G

G

D

E

R

G

V

A

R
x
K

I

V

G
|
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

R

I

D

D

V

V

P

P

H

P

D

R

C

T

T

T

V

A

V
|
V

G

G

I

N

P

P

G

A

R

R

I

L

I

V

binding coenzyme a: H150 (= H109), L169 (≠ Y128), G176 (= G135), K208 (≠ R170), G210 (= G172), A211 (= A173), L216 (= L178), V226 (= V188)

1sstA Serine acetyltransferase- complex with coa (see paper)
39% identity, 64% coverage: 35:195/250 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

D

D

F

I

L

Q

I

A

I

V

F

R

E

H

R

R

D

D

P

P

A

A

A

V

R

E

N

L

W

W

L

S

E

T

V

P

I

L

F

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

I

C

Q

L

S

H

Y

R

R

F

I

A

T

H

H

W

Y

L

L

H

W

R

N

R

Q

G

N

V

R

V

K

F

S

F

L

P

A

R

L

L

Y

I

L

S

Q

H

N

L

Q

A

I

R

S

F

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

K

H

G

G

V

I

F

M

I

F

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

I

V

V

I

G

E

D

N

Y

D

A

V

L

S

I

I

Y
x
L

Q

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

S

-

G

-

K

-

R

-

H

H

P

P

T

K

V

V

G

R

D

E

H

G

V

V

V

M

V

I

G

G

S
x
A

G

G

A

A

K

K

V

I

L
|
L

G

G

N

N

I

I

Q

E

I

V

G

G

D

K

R

Y

V

A

R
x
K

I

I

G
|
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

R

N

D

P

V

V

P

P

H

E

D

Y

C

A

T
|
T

V

A

V
x
A

G

G

I
x
V

P
|
P

G

A

R

R

I

I

I
x
V

binding coenzyme a: H154 (= H109), L173 (≠ Y128), G180 (= G135), T181 (= T136), A193 (≠ S155), L198 (= L160), K208 (≠ R170), G210 (= G172), A211 (= A173), V214 (= V176), L216 (= L178), T224 (= T186), A226 (≠ V188), V228 (≠ I190), P229 (= P191), V233 (≠ I195)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
32% identity, 43% coverage: 89:195/250 of query aligns to 69:181/201 of 1krvA

query
sites
1krvA

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

V

-

I

I

G

G

K

R

G

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

I

T

V

I

G

G

D

D

Y

N

A

V

L

L

I

I

Y
x
A

Q

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

V

I

G

G

D

N

H

N

V

V

V
x
W

V

I

G
|
G

S
|
S

G

H

A

V

K

V

V

I

L
x
N

G

P

N

G

I

V

Q

T

I

I

G

G

D

D

R

N

V

S

R

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

L

T

R

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

I

V

P
|
P

G

C

R
|
R

I

V

I

I

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ L90), Y82 (≠ F106), N84 (≠ D108), V90 (= V114), D92 (≠ G116), M126 (≠ R143)
binding coenzyme a: A104 (≠ Y128), S110 (≠ G134), T112 (= T136), W138 (≠ V152), G140 (= G154), S141 (= S155), N146 (≠ L160), A159 (= A173), P177 (= P191), R179 (= R193)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
32% identity, 43% coverage: 89:195/250 of query aligns to 69:181/201 of 1kruA

query
sites
1kruA

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

V

-

I

I

G

G

K

R

G

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

I

T

V

I

G

G

D

D

Y

N

A

V

L
|
L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

V

I

G

G

D

N

H

N

V

V

V
x
W

V

I

G
|
G

S
|
S

G

H

A

V

K

V

V

I

L

N

G

P

N

G

I

V

Q

T

I

I

G

G

D

D

R

N

V

S

R

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

R

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

I

V

P
|
P

G

C

R

R

I

V

I

I

binding coenzyme a: H114 (vs. gap), W138 (≠ V152), G140 (= G154), S141 (= S155), G158 (= G172), A159 (= A173), P177 (= P191)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: S70 (≠ L90), Y82 (≠ F106), Y82 (≠ F106), N84 (≠ D108), V90 (= V114), D92 (≠ G116), L102 (= L126), H114 (vs. gap), M126 (≠ R143)

Sites not aligning to the query:

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 43% coverage: 89:195/250 of query aligns to 70:182/203 of P07464

query
sites
P07464

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

V

-

I

I

G

G

K

R

G

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

I

T

V

I

G

G

D

D

Y

N

A

V

L

L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

V

I

G

G

D

N

H

N

V

V

V

W

V

I

G

G

S
|
S

G

H

A

V

K

V

V

I

L

N

G

P

N

G

I

V

Q

T

I

I

G

G

D

D

R

N

V

S

R

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

L
x
T

R
x
K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

I

V

P

P

G

C

R
|
R

I

V

I

I

S71 (≠ L90) binding substrate
N85 (≠ D108) binding in other chain; binding in other chain
D93 (≠ G116) binding substrate
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S155) binding in other chain
A160 (= A173) binding in other chain
TK 165:166 (≠ LR 178:179) binding acetyl-CoA
R180 (= R193) binding acetyl-CoA

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
183 binding in other chain

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
32% identity, 43% coverage: 89:195/250 of query aligns to 69:181/200 of 1krrA

query
sites
1krrA

F

F

L

S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

V

-

I

I

G

G

K

R

G

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

I

T

V

I

G

G

D

D

Y

N

A

V

L

L

I
|
I

Y
x
A

Q
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

V

I

G

G

D

N

H

N

V

V

V

W

V

I

G
|
G

S
|
S

G

H

A

V

K

V

V

I

L
x
N

G

P

N

G

I

V

Q

T

I

I

G

G

D

D

R

N

V

S

R

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

R

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V
x
A

G

G

I

V

P
|
P

G

C

R

R

I

V

I

I

binding acetyl coenzyme *a: N84 (≠ D108), I103 (= I127), A104 (≠ Y128), P105 (≠ Q129), T112 (= T136), H114 (vs. gap), G140 (= G154), S141 (= S155), N146 (≠ L160), G158 (= G172), A159 (= A173), A174 (≠ V188), P177 (= P191)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

Query Sequence

>WP_011320646.1 NCBI__GCF_000204075.1:WP_011320646.1
MQQSLDGIENPPDSTHTSLVKNLLSGVFLEPLLSDFLIIFERDPAARNWLEVIFCYPGLH
AICLHRFAHWLHRRGVVFFPRLISHLARFLTGIEIHPGAVIGKGVFIDHGMGVVIGETAI
VGDYALIYQGVTLGGTGKESGKRHPTVGDHVVVGSGAKVLGNIQIGDRVRIGAGSVVLRD
VPHDCTVVGIPGRIITHKPKTDLSPLEHGKLPDVEADVIRSLLSRIEQLEQKLQTLTNQP
KEPELLTKDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory