Comparing WP_011337253.1 NCBI__GCF_000015985.1:WP_011337253.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
1q8jA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima (cd2+, hcy, methyltetrahydrofolate complex) (see paper)
33% identity, 87% coverage: 5:296/335 of query aligns to 7:290/559 of 1q8jA
Sites not aligning to the query:
3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
33% identity, 87% coverage: 5:296/335 of query aligns to 7:290/560 of 3bofA
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
31% identity, 84% coverage: 15:297/335 of query aligns to 14:305/841 of 8g3hA
Sites not aligning to the query:
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
29% identity, 79% coverage: 37:300/335 of query aligns to 68:346/1265 of Q99707
Sites not aligning to the query:
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 77% coverage: 37:295/335 of query aligns to 54:327/1227 of P13009
Sites not aligning to the query:
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
27% identity, 79% coverage: 37:300/335 of query aligns to 51:306/611 of 4cczA
Sites not aligning to the query:
1umyD Bhmt from rat liver (see paper)
27% identity, 76% coverage: 39:293/335 of query aligns to 35:291/381 of 1umyD
O09171 Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5 from Rattus norvegicus (Rat) (see 2 papers)
27% identity, 76% coverage: 39:293/335 of query aligns to 44:314/407 of O09171
Sites not aligning to the query:
1lt8A Reduced homo sapiens betaine-homocysteine s-methyltransferase in complex with s-(delta-carboxybutyl)-l-homocysteine (see paper)
26% identity, 76% coverage: 39:293/335 of query aligns to 34:291/348 of 1lt8A
Sites not aligning to the query:
5dmmA Crystal structure of the homocysteine methyltransferase mmum from escherichia coli, metallated form (see paper)
31% identity, 86% coverage: 5:291/335 of query aligns to 3:286/288 of 5dmmA
Q93088 Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5 from Homo sapiens (Human) (see 4 papers)
26% identity, 76% coverage: 39:293/335 of query aligns to 44:314/406 of Q93088
4m3pA Betaine-homocysteine s-methyltransferase from homo sapiens complexed with homocysteine (see paper)
25% identity, 76% coverage: 39:293/335 of query aligns to 35:283/370 of 4m3pA
Sites not aligning to the query:
>WP_011337253.1 NCBI__GCF_000015985.1:WP_011337253.1
MTDALSRLLRTRDWLMADGATGTNLFNMGLSSGEPPELWTVDRPDNIRSLYRAAVEAGSD
IFLTNSFGGNAARLKLHEAQGRVGELNRIAAELGREVADAAGRPVVVAGSMGPTGEIFEP
MGTLTHRMAVEIFHEQAEALKAGGADVLWVETISAAEEFKAAAEAARLAGMPWCGTMSFD
TAGRTMMGITAAALVDLVTKLPNPPLAFGANCGVGASDLLRTVLGFAAQGIEVPIIAKGN
AGIPKYHDGHIHYDGTPELMAEYAVLARDAGARIIGGCCGTMPEHLKAMRAALEGRPRGP
RPSLETISETLGGFSSASDGSDDAGPTRERRRRRS
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory