SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011371790.1 NCBI__GCF_000012965.1:WP_011371790.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
55% identity, 99% coverage: 1:303/307 of query aligns to 1:305/310 of P9WP55

query
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P9WP55

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K44 (= K42) modified: N6-(pyridoxal phosphate)lysine
N74 (= N72) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTGGS 176:180) binding pyridoxal 5'-phosphate
S266 (= S264) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
55% identity, 99% coverage: 1:303/307 of query aligns to 1:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K42), S266 (= S264), P293 (≠ C291)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K42), T71 (= T69), S72 (= S70), N74 (= N72), T75 (= T73), Q144 (= Q142), V177 (= V175), G178 (= G176), T179 (= T177), G180 (= G178), T182 (≠ S180), G222 (= G220), I223 (= I221), S266 (= S264), P293 (≠ C291), D294 (= D292)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
56% identity, 97% coverage: 1:298/307 of query aligns to 1:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K42), S266 (= S264), P293 (≠ C291)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T69), S72 (= S70), I126 (≠ V124), Q144 (= Q142), F145 (= F143), K215 (≠ A213), G222 (= G220), A225 (= A223), F227 (= F225)
binding pyridoxal-5'-phosphate: K44 (= K42), N74 (= N72), V177 (= V175), G178 (= G176), T179 (= T177), G180 (= G178), T182 (≠ S180), G222 (= G220), S266 (= S264), P293 (≠ C291), D294 (= D292)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
56% identity, 97% coverage: 1:298/307 of query aligns to 1:300/300 of 2q3cA

query
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2q3cA

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L

I

I

V

V

T

V

I

V

L

L

C
x
P

D

D

T

F

A

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S264), P293 (≠ C291)
binding : T71 (= T69), S72 (= S70), G73 (= G71), T75 (= T73), M122 (= M120), Q144 (= Q142), K215 (≠ A213), G222 (= G220), A225 (= A223)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
56% identity, 100% coverage: 1:307/307 of query aligns to 1:312/318 of 4lmaA

query
sites
4lmaA

M

M

K

R

Y

I

A

A

K

H

N

D

V

V

T

T

E

E

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

I

L

N

N

I

R

A

I

S

P

Q

Q

N

A

A

E

L

G

V

C

L

L

G

G

-

R

-

I

-

V

-

M

K

K

C

L

E

E

F

G

M

M

N

N

P

P

T

A

H

A

S

S

I

V

K
|
K

D

D

R

R

I

I

G

G

K

T

Y

H

M

M

I

I

Q

N

E

S

A

A

L

E

T

K

Q

A

G

G

L

L

I

I

T

N

K

P

N

E

T

T

V

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

M

V

T

C

A

A

A

S

A

L

K

G

G

I

Y

K

R

L

L

I

I

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

K

L

E

E

R

R

I

R

Q

A

L

M

L

L

R

K

A

A

L

Y

G

G

A

A

R

T

V

L

V

E

L

L

T

T

D

P

A

G

K

S

Q

Q

G

G

M

M

M

K

G

G

A

A

V

I

R

L

E

R

A

A

K

Q

E

Q

L

I

C

V

S

D

Q

S

H

I

S

P

E

G

A

A

L

Y

M

M

L

L

Q

Q

F

F

S

R

N

N

R

P

A

S

N

N

P

P

E

E

I

I

H

H

R

R

K

L

T

T

A

A

L

E

E

E

I

I

I

W

R

Q

D

D

T

T

Q

E

G

G

K

Q

V

V

D

D

I

F

L

I

V

V

L

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

V

V

G

A

E

E

V

V

L

I

K

K

K

S

H

R

N

K

P

P

N

S

I

F

K

Q

I

V

A

A

V

V

E

E

P

P

E

F

N

N

S

S

P

P

V

V

L

I

S

S

G

G

E

N

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

E

I

V

L

L

N

R

T

T

E

D

I

L

Y

I

D

D

E

E

I

V

I

I

R

T

V

V

S

S

G

D

E

E

D

E

A

A

I

F

N

Q

C

F

S

G

R

R

E

R

M

L

A

A

K

K

K

E

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

N

N

V

L

F

C

A

A

S

A

T

I

Q

Q

A

L

A

A

K

Q

R

R

Q

P

E

E

N

N

R

E

G

G

K

K

T

L

I

I

V

V

T

V

I

I

L

Q

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

L

L

M

Y

F

D

Q

E

N

D

I

E

E

active site: K46 (= K42), S269 (= S264)
binding pyridoxal-5'-phosphate: K46 (= K42), N77 (= N72), G181 (= G176), T182 (= T177), G183 (= G178), T185 (≠ S180), G225 (= G220), S269 (= S264), P296 (≠ C291)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
53% identity, 99% coverage: 1:303/307 of query aligns to 11:316/323 of 4aecA

query
sites
4aecA

M

L

K

N

Y

I

A

A

K

D

N

N

V

V

T

S

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

I

L

N

N

I

S

A

I

S

A

Q

K

N

G

-

C

-

V

A

A

L

N

V

I

L

A

G

A

K

K

C

L

E

E

F

I

M

M

N

E

P

P

T

C

H

C

S

S

I

V

K
|
K

D

D

R

R

I

I

G

G

K

Y

Y

S

M

M

I

V

Q

T

E

D

A

A

L

E

T

Q

Q

K

G

G

L

F

I

I

T

S

K

P

N

G

T

K

T

S

V

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

S

S

L

R

G

G

I

Y

K

R

L

L

I

I

L

L

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

M

E

E

R

R

I

R

Q

V

L

L

R

K

A

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

T

G

G

A

A

V

V

R

Q

E

K

A

A

K

E

E

E

L

I

C

L

S

K

Q

N

H

T

S

P

E

D

A

A

L

Y

M

M

L

L

Q

Q

F

F

S

D

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

D

D

D

T

T

Q

K

G

G

K

K

V

V

D

D

I

I

L

F

V

V

L

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

S
x
T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

K

E

H

K

N

N

P

P

N

K

I

T

K

Q

I

V

I

I

A

G

V

V

E

E

P

P

E

T

N

E

S

S

P

D

V

I

L

L

S

S

G

G

E

K

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

I

N

L

L

N

D

T

Q

E

K

I

I

Y

M

D

D

E

E

I

V

I

I

R

A

V

I

S

S

G

S

E

E

D

E

A

A

I

I

N

E

C

T

S

A

R

K

E

Q

M

L

A

A

K

L

K

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q

K

A

V

A

A

K

K

R

R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

I

V

A

T

V

I

V

L

F

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

P

L

L

Y

F

active site: K54 (= K42), S277 (= S264)
binding pyridoxal-5'-phosphate: K54 (= K42), N85 (= N72), I188 (≠ V175), G189 (= G176), T190 (= T177), G191 (= G178), G192 (= G179), T193 (≠ S180), G233 (= G220), S277 (= S264), P304 (≠ C291)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
55% identity, 98% coverage: 4:304/307 of query aligns to 4:307/309 of 7n2tA

query
sites
7n2tA

A

A

K

K

N

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

-

R

-

V

I

V

A

D

S

G

Q

C

N

V

A

A

L

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

I

V

K

K

D

D

R

R

I

I

G

G

K

Y

Y

S

M

M

I

I

Q

S

E

D

A

A

L

E

T

N

Q

K

G

G

L

L

I

I

T

T

K

P

N

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

S

A

L

K

G

G

I

Y

K

R

L

L

I

I

L

I

T

C

M

M

P

P

S

A

S

S

M

M

S

S

K

L

E

E

R

R

I

R

Q

T

L

I

L

L

R

R

A

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

D

D

A

P

K

A

Q

R

G

G

M

M

M

K

G

G

A

A

V

V

R

Q

E

K

A

A

K

E

E

E

L

I

C

K

S

A

Q

K

H

T

S

P

E

N

A

S

L

Y

M

I

L

L

Q

Q

F

F

S

E

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

R

D

G

T

S

Q

G

G

G

K

K

V

I

D

D

I

A

L

L

V

V

L

S

G

G

V

I

G

G

T

T

G

G

S

T

I

V

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

H

Q

N

N

P

P

N

N

I

I

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

E

V

N

E

S

S

P

A

V

I

L

L

S

S

G

G

E

K

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

G

I

V

L

L

N

D

T

V

E

N

I

L

Y

L

D

D

E

E

I

V

I

I

R

Q

V

V

S

S

G

S

E

E

D

E

A

S

I

I

N

E

C

T

S

A

R

K

E

L

M

L

A

A

K

L

K

K

E

E

G

G

L

L

V

V

G

G

I

I

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q
x
R

A

I

A

A

K

K

R
|
R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

I

V

V

T

A

I

V

L

F

C

P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

V

L

L

Y

F

D

E

binding phosphate ion: R277 (≠ Q274), R281 (= R278)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
53% identity, 99% coverage: 2:304/307 of query aligns to 2:307/320 of 2isqA

query
sites
2isqA

K

R

Y

I

A

A

K

K

N

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

I

N

A

V

S

A

Q

E

N

G

A

C

L

V

-

G

-

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

I

V

K
|
K

D

D

R

R

I

I

G

G

K

F

Y

S

M

M

I

I

Q

S

E

D

A

A

L

E

T

K

Q

K

G

G

L

L

I

I

T

K

K

P

N

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

S

A

L

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

T

E

E

R

R

I

R

Q

I

L

I

L

L

R

L

A

A

L

F

G

G

A

V

R

E

V

L

V

V

L

L

T

T

D

D

A

P

K

A

Q

K

G
|
G

M
|
M

M
x
K

G

G

A

A

V

I

R

A

E

K

A

A

K

E

E

E

L

I

C

L

S

A

Q

K

H

T

S

P

E

N

A

G

L

Y

M

M

L

L

Q

Q

F

F

S

E

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

K

D

G

T

T

Q

G

G

G

K

K

V

I

D

D

I

G

L

F

V

V

L

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

H

Q

N

N

P

A

N

N

I

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

E

V

N

E

S

S

P

A

V

I

L

L

S

S

G

G

E

K

A

P

G
|
G

P
|
P

H
|
H

E

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

I

V

L

L

N

N

T

V

E

D

I

L

Y

I

D

D

E

E

I

V

R

Q

V

V

S

S

G

S

E

D

D

E

A

S

I

I

N

D

C

M

S

A

R

R

E

Q

M

L

A

A

K

L

K

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q

K

A

L

A

A

K

Q

R

R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

V

L

L

Y

F

D

D

active site: K44 (= K42), S267 (= S264)
binding pyridoxal-5'-phosphate: K44 (= K42), N75 (= N72), G177 (= G174), G179 (= G176), T180 (= T177), G181 (= G178), T183 (≠ S180), G223 (= G220), S267 (= S264), P294 (≠ C291)
binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), G122 (= G119), M123 (= M120), K124 (≠ M121), G217 (= G214), P218 (= P215), H219 (= H216), Q222 (= Q219), G223 (= G220)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
53% identity, 99% coverage: 2:304/307 of query aligns to 4:309/322 of P47998

query
sites
P47998

K

R

Y

I

A

A

K

K

N

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

I

N

A

V

S

A

Q

E

N

G

A

C

L

V

-

G

-

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

I

V

K
|
K

D

D

R

R

I

I

G

G

K

F

Y

S

M

M

I

I

Q

S

E

D

A

A

L

E

T

K

Q

K

G

G

L

L

I

I

T

K

K

P

N

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

S

A

L

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

T

E

E

R

R

I

R

Q

I

L

I

L

L

R

L

A

A

L

F

G

G

A

V

R

E

V

L

V

V

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

K

G

G

A

A

V

I

R

A

E

K

A

A

K

E

E

E

L

I

C

L

S

A

Q

K

H

T

S

P

E

N

A

G

L

Y

M

M

L

L

Q

Q

Q
|
Q

F

F

S

E

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H
|
H

R

Y

K

E

T

T

A
x
G

L

P

E

E

I

I

I

W

R

K

D

G

T

T

Q

G

G

G

K

K

V

I

D

D

I

G

L

F

V

V

L

S

G

G

V

I

G
|
G

T
|
T

G
|
G

S
x
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

H

Q

N

N

P

A

N

N

I

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

E

V

N

E

S

S

P

A

V

I

L

L

S

S

G

G

E
x
K

A

P

G

G

P

P

H
|
H

E
x
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

I

V

L

L

N

N

T

V

E

D

I

L

Y

I

D

D

E

E

I

V

R

Q

V

V

S

S

G

S

E

D

D

E

A

S

I

I

N

D

C

M

S

A

R

R

E

Q

M

L

A

A

K

L

K

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q

K

A

L

A

A

K

Q

R

R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C

P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

V

L

L

Y

F

D

D

K46 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T69) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S70) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N72) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T73) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q142) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H152) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A157) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTGGS 176:180) binding pyridoxal 5'-phosphate
T182 (= T177) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (≠ S180) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ E212) mutation to A: Impaired interaction with SAT1.
H221 (= H216) mutation to A: Impaired interaction with SAT1.
K222 (≠ E217) mutation to A: Impaired interaction with SAT1.
S269 (= S264) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
52% identity, 99% coverage: 1:305/307 of query aligns to 1:310/310 of 4lmbA

query
sites
4lmbA

M

M

K

L

Y

I

A

A

K

R

N

D

V

I

T

T

E

Q

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

I

L

N

N

-

R

-

I

-

P

-

V

I

A

A

E

S

G

Q

V

N

K

A

A

L

R

V

I

L

V

G

V

K

K

C

L

E

E

F

S

M

M

N

N

P

P

T

A

H

A

S

S

I

V

K
|
K

D

D

R

R

I

I

G

G

K

V

Y

S

M

M

I

V

Q

E

E

D

A

A

L

E

T

A

Q

A

G

G

L

L

I

I

T

H

K

P

N

D

T

K

T

T

V

I

-

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

A

A

A

S

A

L

K

G

G

I

Y

K

R

L

L

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M
|
M

S

S

K

L

E

E

R

R

I

R

Q

A

L

M

L

L

R

K

A

A

L

Y

G

G

A

A

R

Q

V

L

V

E

L

L

T

T

D

P

A

G

K

S

Q

Q

G

G

M
|
M

M

E

G

G

A

A

V

I

R

T

E

R

A

A

K

E

E

E

L

I

C

V

S

E

Q

N

H

T

S

P

E

N

A

A

L

Y

M

S

L

L

Q

Q

Q
|
Q

F
|
F

S

R

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

R

K

E

T

T

A

A

L

E

E

E

I

I

I

W

R

A

D

D

T

T

Q

D

G

G

K

L

V

V

D

D

I

I

L

V

V

I

L

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

V

I

G

A

E

E

V

T

L

I

K

K

K

P

H

R

N

R

P

P

N

Q

I

F

K

Q

I

A

I

I

A

A

V

V

E

E

P

P

E

S

N

N

S

S

P

P

V

V

L

L

S

S

G

G

E

Q

A

P

G

G

P

P

H

H

E

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

A

I

I

L

F

N

R

T

P

E

E

I

L

Y

I

D

D

E

E

I

V

I

I

R

I

V

V

S

D

G

D

E

T

D

E

A

A

I

F

N

A

C

Y

S

A

R

R

E

R

M

L

A

A

K

R

K

Q

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

N

A

V

L

F

W

A

A

S

A

T

I

Q

Q

A

V

A

G

K

K

R

R

Q

P

E

E

N

N

R

E

G

D

K

K

T

L

I

I

V

V

T

M

I

I

L

Q

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

A

L

L

Y

F

D

K

E

D

active site: K46 (= K42), S269 (= S264)
binding cysteine: K46 (= K42), T74 (= T69), S75 (= S70), N77 (= N72), T78 (= T73), M101 (= M96), M125 (= M120), M125 (= M120), Q147 (= Q142), F148 (= F143), Q224 (= Q219), G225 (= G220), G225 (= G220), I226 (= I221), A228 (= A223)
binding pyridoxal-5'-phosphate: K46 (= K42), N77 (= N72), V180 (= V175), G181 (= G176), T182 (= T177), G183 (= G178), T185 (≠ S180), G225 (= G220), S269 (= S264), P296 (≠ C291)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
52% identity, 99% coverage: 2:304/307 of query aligns to 2:307/320 of 1z7yA

query
sites
1z7yA

K

R

Y

I

A

A

K

K

N

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

I

N

A

V

S

A

Q

E

N

G

A

C

L

V

-

G

-

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

I

V

K
x
A

D

D

R

R

I

I

G

G

K

F

Y

S

M

M

I

I

Q

S

E

D

A

A

L

E

T

K

Q

K

G

G

L

L

I

I

T

K

K

P

N

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

S

A

L

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

T

E

E

R

R

I

R

Q

I

L

I

L

L

R

L

A

A

L

F

G

G

A

V

R

E

V

L

V

V

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

K

G

G

A

A

V

I

R

A

E

K

A

A

K

E

E

E

L

I

C

L

S

A

Q

K

H

T

S

P

E

N

A

G

L

Y

M

M

L

L

Q

Q

Q
|
Q

F

F

S

E

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

K

D

G

T

T

Q

G

G

G

K

K

V

I

D

D

I

G

L

F

V

V

L

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

H

Q

N

N

P

A

N

N

I

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

E

V

N

E

S

S

P

A

V

I

L

L

S

S

G

G

E

K

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

I

V

L

L

N

N

T

V

E

D

I

L

Y

I

D

D

E

E

I

V

R

Q

V

V

S

S

G

S

E

D

D

E

A

S

I

I

N

D

C

M

S

A

R

R

E

Q

M

L

A

A

K

L

K

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q

K

A

L

A

A

K

Q

R

R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C
x
P

D
x
S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

V

L

L

Y

F

D

D

active site: A44 (≠ K42), S267 (= S264)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G71), N75 (= N72), T76 (= T73), Q145 (= Q142), I178 (≠ V175), G179 (= G176), T180 (= T177), G181 (= G178), T183 (≠ S180), G223 (= G220), S267 (= S264), P294 (≠ C291), S295 (≠ D292)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 98% coverage: 4:303/307 of query aligns to 76:378/392 of P47999

query
sites
P47999

A

A

K

D

N

N

V

A

T

A

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

I

L

N

N

-

N

-

V

I

V

A

K

S

G

Q

C

N

V

A

A

L

S

V

V

L

A

G

A

K

K

C

L

E

E

F

I

M

M

N

E

P

P

T

C

H

C

S

S

I

V

K

K

D

D

R

R

I

I

G

G

K

Y

Y

S

M

M

I

I

Q

T

E

D

A

A

L

E

T

E

Q

K

G

G

L

L

I

I

T

T

K

P

N

G

T

K

T

S

V

V

-

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

S

S

L

K

G

G

I

Y

K

K

L

L

I

I

L

L

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

L

E

E

R

R

I

R

Q

V

L

L

R

R

A

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

D

E

A

P

K

A

Q

K

G

G

M

M

M

T

G

G

A

A

V

I

R

Q

E

K

A

A

K

E

E

E

L

I

C

L

S

K

Q

K

H

T

S

P

E

N

A

S

L

Y

M

M

L

L

Q

Q

F

F

S

D

N

N

R

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

D

T

T

Q

R

G

G

K

K

V

I

D

D

I

I

L

L

V

V

L

A

G

G

V

I

G

G

T

T

G

G

S

T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

K

E

H

R

N

K

P

P

N

E

I

L

K

K

I

V

I

I

A

G

V

V

E

E

P

P

E

T

N

E

S

S

P

A

V

I

L

L

S

S

G

G

E

K

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

K

I

N

L

L

N

D

T

L

E

A

I

I

Y

V

D

D

E

E

I

Y

I

I

R

A

V

I

S

S

G

S

E

E

D

E

A

A

I

I

N

E

C

T

S

S

R

K

E

Q

M

L

A

A

K

L

K

Q

E

E

G

G

L

L

V

V

G

G

I

I

S

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

Q

Q

A

V

A

A

K

K

R

R

Q

P

E

E

N

N

R

A

G

G

K

K

T

L

I

I

V

A

T

V

I

V

L

F

C

P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

Q

L

L

Y

F

Sites not aligning to the query:

61 modified: N-acetylalanine

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
51% identity, 98% coverage: 5:305/307 of query aligns to 13:316/329 of 3vbeC

query
sites
3vbeC

K

K

N

H

V

V

T

S

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

I

K

A

V

S

T

Q

E

N

G

-

C

-

G

A

A

L

Y

V

V

L

A

G

V

K

K

C

Q

E

E

F

M

M

M

N

Q

P

P

T

T

H

A

S

S

I

I

K
|
K

D

D

R

R

I

P

G

A

K

Y

Y

A

M

M

I

I

Q

T

E

D

A

A

L

E

T

E

Q

K

G

N

L

L

I

I

T

T

K

P

-

G

N

K

T

T

V

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

S

F

V

M

C

A

A

A

S

M

L

K

G

G

I

Y

K

K

L

M

I

V

L

L

T

T

M

M

P

P

S

S

S

Y

M

T

S

S

K

L

E

E

R

R

I

R

Q

V

L

T

L

M

R

R

A

A

L

F

G

G

A

A

R

E

V

L

V

I

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

G

G

G

A

T

V

V

R

K

E

K

A

A

K

Y

E

E

L

L

C

L

S

E

Q

N

H

T

S

P

E

N

A

A

L

H

M

M

L

L

Q

Q

F

F

S

S

N

N

R

P

A

A

N

N

P

T

E

Q

I

V

H

H

R

F

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

D

T

T

Q

N

G

G

K

Q

V

V

D

D

I

I

L

F

V

V

L
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

S
x
T

I

V

T

S

G

G

V

V

G

G

E

Q

V

Y

L

L

K

K

K

S

H

K

N

N

P

P

N

N

I

V

K

K

I

I

I

Y

A

G

V

V

E
|
E

P

P

E

S

N

E

S
|
S

P

N

V

V

L

L

S

N

G

G

E

K

A

P

G

G

P

P

H

H

E

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

I

K

P

P

E

D

I

I

L

L

N

D

T

L

E

D

I

V

Y

M

D

E

E

K

I

V

I

L

R

E

V

V

S

S

G

S

E

E

D

D

A

A

I

V

N

N

C

M

S

A

R

R

E

V

M

L

A

A

K

L

K

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

N

V

T

F

V

A

A

S

A

T

L

Q

R

A

L

A

A

K

Q

R

L

Q

P

E

E

N

N

R

K

G

G

K

K

T

L

I

I

V

V

T

T

I

V

L

H

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

S

G

V

L

L

Y

F

D

Q

E

E

active site: K52 (= K42), S81 (= S70), E212 (= E201), S216 (= S205), S275 (= S264), P302 (≠ C291)
binding pyridoxal-5'-phosphate: K52 (= K42), N83 (= N72), M184 (≠ L173), G187 (= G176), S188 (≠ T177), G189 (= G178), T191 (≠ S180), G231 (= G220), S275 (= S264), P302 (≠ C291)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
51% identity, 98% coverage: 5:305/307 of query aligns to 6:309/322 of 3vc3A

query
sites
3vc3A

K

K

N

H

V

V

T

S

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

I

L

N

N

I

K

A

V

S

T

Q

E

N

G

-

C

-

G

A

A

L

Y

V

V

L

A

G

V

K

K

C

Q

E

E

F

M

M

M

N

Q

P

P

T

T

H

A

S

S

I

I

K
x
A

D

D

R

R

I

P

G

A

K

Y

Y

A

M

M

I

I

Q

T

E

D

A

A

L

E

T

E

Q

K

G

N

L

L

I

I

T

T

K

P

-

G

N

K

T

T

V

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
x
M

G

G

I

I

A

S

L

M

A

A

S

F

V

M

C

A

A

A

S

M

L

K

G

G

I

Y

K

K

L

M

I

V

L

L

T

T

M

M

P

P

S

S

S

Y

M

T

S

S

K

L

E

E

R

R

I

R

Q

V

L

T

L

M

R

R

A

A

L

F

G

G

A

A

R

E

V

L

V

I

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

G

G

G

A

T

V

V

R

K

E

K

A

A

K

Y

E

E

L

L

C

L

S

E

Q

N

H

T

S

P

E

N

A

A

L

H

M

M

L

L

Q

Q

Q
|
Q

F

F

S

S

N

N

R

P

A

A

N

N

P

T

E

Q

I

V

H

H

R

F

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

D

T

T

Q

N

G

G

K

Q

V

V

D

D

I

I

L

F

V

V

L
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

S
x
T

I

V

T

S

G

G

V

V

G

G

E

Q

V

Y

L

L

K

K

K

S

H

K

N

N

P

P

N

N

I

V

K

K

I

I

I

Y

A

G

V

V

E

E

P

P

E

S

N

E

S

S

P

N

V

V

L

L

S

N

G

G

E

K

A

P

G

G

P

P

H

H

E

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

I

K

P

P

E

D

I

I

L

L

N

D

T

L

E

D

I

V

Y

M

D

E

E

K

I

V

I

L

R

E

V

V

S

S

G

S

E

E

D

D

A

A

I

V

N

N

C

M

S

A

R

R

E

V

M

L

A

A

K

L

K

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

N

V

T

F

V

A

A

S

A

T

L

Q

R

A

L

A

A

K

Q

R

L

Q

P

E

E

N

N

R

K

G

G

K

K

T

L

I

I

V

V

T

T

I

V

L

H

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

S

G

V

L

L

Y

F

D

Q

E

E

active site: A45 (≠ K42), S268 (= S264), P295 (≠ C291)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T69), S74 (= S70), N76 (= N72), M77 (≠ T73), Q146 (= Q142), M177 (≠ L173), G180 (= G176), S181 (≠ T177), G182 (= G178), T184 (≠ S180), G224 (= G220), S268 (= S264), P295 (≠ C291)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
52% identity, 98% coverage: 5:305/307 of query aligns to 9:312/341 of Q93244

query
sites
Q93244

K

E

N

N

V

A

T

A

E

A

L

L

I

I

G

G

N

N

T

T

P

P

L

M

V

V

R

Y

I

I

N

N

I

K

A

L

S

T

Q

K

N

G

-

L

-

P

A

G

L

T

V

V

L

A

G

V

K

K

C

I

E

E

F

Y

M

M

N

N

P

P

T

A

H

G

S

S

I

V

K

K

D

D

R

R

I

I

G

G

K

A

Y

A

M

M

I

L

Q

A

E

A

A

A

L

E

T

K

Q

D

G

G

-

T

L

V

I

I

T

P

K

G

N

V

T

T

V

L

I

I

E

E

P
|
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

S

F

V

V

C
x
A

A

A

S

A

L

K

G

G

I

Y

K

R

L

C

I

I

L

V

T

T

M

M

P

P

S

A

S

S

M

M

S

S

K

G

E

E

R

R

I

R

Q

T

L

L

R

K

A

A

L

Y

G

G

A

S

R

E

V

V

L

L

T

T

D

D

A

P

K

A

Q

K

G

G

M

M

M

K

G

G

A

A

V

I

R

D

E

M

A

A

K

N

E

Q

L

L

C

K

S

E

Q

N

H

I

S

P

E

G

A
x
S

L

I

M

I

L

L

Q

A

Q

Q

F

F

S

D

N

N

R

P

A

N

N

N

P

P

E

L

I

V

H

H

R

Y

K

Q

T

T

A

G

L

P

E

E

I

I

I

W

R

R

D

Q

T

T

Q

K

G

G

K

T

V

V

D

D

I

A

L

V

V

V

L

F

G
|
G

V

V

G
|
G

T

T

G

G

S

T

I

I

T

T

G

G

V

V

G

G

E

R

V

Y

L

L

K

Q

K

E

H

Q

N

N

P

P

N

G

I

V

K

R

I

V

I

F

A

A

V

V

E

E

P

P

E

E

N

E

S

S

P

A

V

I

L

L

S

S

G

G

G

R

E

P

A

A

G

G

P

P

H

H

E

K

I

I

Q

Q

G

G

I

I

G
|
G

A

A

G

G

F

F

I

A

P

P

E

A

I

V

L

L

N

D

T

T

E

K

I

I

Y

Y

D

E

E

D

I

V

I

I

R

R

V

I

S

H

G

S

E

D

D

E

A

A

I

I

N

V

C

M

S

A

R

Q

E
x
R

M

L

A

S

K

Y

K

E

E

E

G

G

L

L

V

G

G

G

I

I

S

S

S
|
S

G

G

A

A

N

N

V

V

F

A

A

A

S

A

T

L

Q

Q

A

L

A

A

K

A

R

R

Q

P

E

E

N

M

R

A

G

G

K

K

T

L

I

I

V

V

T
|
T

I

C

L

L

C

P

D

S

T

C

A

G

E

E

R

R

Y

Y

L

M

S

T

C

S

G

P

L

L

Y

Y

D

T

E

D

P75 (= P68) mutation to L: In n5537; severe loss of protein stability.
A88 (≠ C81) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ A137) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G174) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G176) mutation to R: In n5515; severe loss of protein stability.
G229 (= G222) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ E252) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S265) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (= T288) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
49% identity, 97% coverage: 7:305/307 of query aligns to 32:333/347 of Q9FS29

query
sites
Q9FS29

V

V

T

S

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

I

L

N

K

I

K

A

V

S

T

Q

E

N

G

-

C

-

G

A

A

L

Y

V

I

L

A

G

V

K

K

C

Q

E

E

F

M

M

F

N

Q

P

P

T

T

H

S

S

S

I

I

K

K

D

D

R

R

I

P

G

A

K

L

Y

A

M

M

I

I

Q

N

E

D

A

A

L

E

T

K

Q

K

G

G

L

L

I

I

T

S

-

P

K

E

N

K

T

T

V

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

S

F

V

M

C

A

A

A

S

M

L

K

G

G

I

Y

K

K

L

M

I

V

L

L

T

T

M

M

P

P

S

S

S

Y

M

T

S

S

K

M

E

E

R

R

I

R

Q

V

L

T

L

M

R

R

A

A

L

F

G

G

A

A

R

D

V

L

V

I

L

L

T

T

D

D

A

P

K

T

Q

K

G

G

M

M

M

G

G

G

A

T

V

V

R

K

E

K

A

A

K

Y

E
|
E

L

L

C

L

S

E

Q

S

H

T

S

P

E

N

A

A

L

F

M

M

L

L

Q

Q

F

F

S

S

N

N

R

P

A

A

N

N

P

T

E

Q

I

V

H

H

R

F

K

D

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

E

T

T

Q

L

G

G

K

N

V

V

D

D

I

I

L

F

V

V

L

M

G

G

V

I

G

G

T

S

G

G

S

T

I

V

T

T

G

G

V

V

G

G

E

L

V

Y

L

L

K

K

K

S

H

K

N

N

P

P

N

N

I

V

K

K

I

I

I

Y

A

G

V

L

E

E

P

P

E

T

N

E

S

S

P

N

V

I

L

L

S

N

G

G

E

K

A

P

G

G

P

P

H

H

E

H

I

I

Q

T

G

G

I

N

G

G

A

V

G

G

F

S

I

K

P

P

E

D

I

I

L

V

N

D

T

M

E

D

I

L

Y

M

D

E

E

E

I

V

I

L

R

M

V

V

S

S

G

S

E

E

D

D

A

A

I

V

N

N

C

M

S

A

R

R

E

E

M

L

A

A

K

V

K

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S

S

G

G

A

A

N

N

V

T

F

V

A

A

S

A

T

L

Q

R

A

L

A

A

K

Q

R

K

Q

P

E

E

N

N

R

K

G

G

K

K

T

L

I

I

V

V

T

T

I

V

L

H

C

A

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

S

G

V

L

L

Y

Y

D

Q

E

D

E157 (= E129) mutation E->N,Q: No effect on catalytic activities.

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
51% identity, 99% coverage: 1:304/307 of query aligns to 2:307/310 of 5xoqA

query
sites
5xoqA

M

M

K

P

Y

I

A

F

K

K

N

D

V

N

T

S

E

E

L

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

I

I

N

N

-

R

-

L

I

T

A

A

S

G

Q

L

N

S

A

S

L

R

V

V

L

L

G

A

K

K

C

I

E

E

F

G

M

R

N

N

P

P

T

A

H

Y

S

S

I

V

K

K

D

C

R

R

I

I

G

G

K

A

Y

A

M

M

I

I

Q

W

E

D

A

A

L

E

T

Q

Q

S

G

G

L

K

I

L

T

K

K

P

N

G

T

M

T

H

V

V

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

F

V

V

C

C

A

A

S

A

L

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

K

I

E

E

R

R

I

R

Q

M

L

M

L

L

R

K

A

S

L

F

G

G

A

A

R

D

V

L

V

V

L

L

T

T

D

P

A

G

K

A

Q

D

G

G

M
|
M

M

K

G

G

A

A

V

I

R

S

E

K

A

A

K

E

E

E

L

L

C

A

S

A

Q

Q

H

P

S

G

E

W

A

-

L

F

M

I

L

P

Q

Q

Q
|
Q

F

F

S

K

N

N

R

P

A

A

N

N

P

P

E

A

I

I

H

H

R

V

K

K

T

T

A

G

L

P

E

E

I

I

I

W

R

N

D

D

T

T

Q

E

G

G

K

Q

V

V

D

D

I

V

L

F

V

V

L

A

G

G

V

V

G

G

T

T

G

G

S

T

I

I

T

T

G

G

V

V

G

A

E

R

V

F

L

L

K

K

-

H

-

E

K

K

H

K

N

H

P

P

N

-

I

V

K

H

I

V

A

A

V

V

E

E

P

P

E

A

N

A

S

S

P

P

V

V

L

L

S

A

G

G

E

P

A

A

G

G

P
x
R

H
|
H

E

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

E

D

I

T

L

F

N

D

T

R

E

S

I

V

Y

V

D

D

E

E

I

I

I

L

R

S

V

V

S

T

G

D

E

D

D

E

A

A

I

I

N

E

C

T

S

A

R

R

E

K

M

L

A

A

K

M

K

E

E

E

G

G

L

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

N

A

V

M

F

A

A

G

S

A

T

L

Q

K

A

V

A

A

K

A

R

R

Q

P

E

E

N

F

R

A

G

G

K

K

T

T

I

I

V

V

T

T

I

V

L

L

C

P

D

D

T

A

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

A

L

L

Y

F

D

E

binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), M123 (= M120), Q144 (= Q142), R218 (≠ P215), H219 (= H216), Q222 (= Q219), G223 (= G220), A226 (= A223)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
50% identity, 98% coverage: 4:303/307 of query aligns to 10:311/329 of 8b9wA

query
sites
8b9wA

A

A

K

P

N

S

V

V

T

D

E

A

L

L

I

I

G

G

N

N

T

T

P

P

L

A

V

V

R

Y

I

L

-

R

N

N

I

M

A

N

S

K

Q

T

N

A

A

A

L

T

V

I

L

V

G

L

K

K

C

L

E

E

F

S

M

E

N

N

P

P

T

M

H

A

S

S

I

V

K
|
K

D

D

R

R

I

L

G

A

K

Y

Y

A

M

I

I

Y

Q

D

E

K

A

A

L

E

T

K

Q

E

G

G

L

K

I

I

T

K

K

R

N

G

T

K

T

S

V

V

I

I

-

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

L

V

L

C

G

A

A

S

V

L

R

G

G

I

Y

K

K

L

V

I

I

L

I

T

T

M

M

P

P

S

D

S

S

M

M

S

S

K

M

E

E

R

R

I

R

Q

S

L

L

R

R

A

I

L

F

G

G

A

A

R

E

V

L

V

I

L

L

T

T

D

P

A

A

K

S

Q

L

G

G

M

M

M

K

G

G

A

A

V

V

R

A

E

M

A

A

K

N

E

R

L

I

C

V

S

S

Q

N

H

N

S

P

E

D

A

A

L

V

M

L

L

A

Q

D

Q

Q

F

F

S

C

N

T

R

K

A

Y

N

N

P

A

E

Q

I

I

H

H

R

E

K

E

T

T

A

G

L

P

E

E

I

I

I

W

R

R

D

Q

T

T

Q

K

G

G

K

H

V

V

D

D

I

C

L

F

V

V

L

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

L

T

T

G

G

V

V

G

A

E

R

V

F

L

L

K

K

K

S

H

V

N

G

P

C

N

N

I

A

K

N

I

I

I

V

A

A

V

V

E

E

P

P

E

Q

N

E

S

S

P

P

V

V

L

L

S

S

G

G

E

R

A

P

G

G

P

P

H

H

E

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

E

I

V

L

L

N

D

T

R

E

E

I

L

Y

V

D

D

E

E

I

I

R

Q

V

V

S

S

G

S

E

D

D

K

A

A

I

I

N

E

C

T

S

A

R

Q

E

K

M

L

A

S

K

R

K

M

E

D

G

G

L

I

L

F

V

C

G

G

I

F

S
|
S

S

A

G

G

A

A

N

N

V

V

F

F

A

A

S

A

T

L

Q

K

A

I

A

A

K

E

R

R

Q

P

E

E

N

M

R

A

G

G

K

K

T

T

I

I

V

V

T

T

I

V

L

I

C
x
P

D

S

T

F

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

A

L

L

Y

Y

binding pyridoxal-5'-phosphate: K49 (= K42), N80 (= N72), G184 (= G176), T185 (= T177), G186 (= G178), T188 (≠ S180), G228 (= G220), S272 (= S264), P299 (≠ C291)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
50% identity, 95% coverage: 11:303/307 of query aligns to 12:312/323 of P0ABK5

query
sites
P0ABK5

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

I

L

N

N

I

R

A

I

S

G

Q

-

N

N

A

G

L

R

V

I

L

L

G

A

K

K

C

V

E

E

F

S

M

R

N

N

P

P

T

S

H

F

S

S

I

V

K
|
K

D

C

R

R

I

I

G

G

K

A

Y

N

M

M

I

I

Q

W

E

D

A

A

L

E

T

K

Q

R

G

G

L

V

I

L

T

K

K

P

N

G

T

V

T

E

V

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

S

Y

V

V

C

A

A

A

S

A

L

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

K

I

E

E

R

R

I

R

Q

K

L

L

R

K

A

A

L

L

G

G

A

A

R

N

V

L

V

V

L

L

T

T

D

E

A

G

K

A

Q

K

G

G

M

M

M

K

G

G

A

A

V

I

R

Q

E

K

A

A

K

E

E

E

L

I

C

V

S

A

Q

S

H

N

S

P

E

E

A

K

-

Y

L

L

M

L

L

L

Q

Q

F

F

S

S

N

N

R

P

A

A

N

N

P

P

E

E

I

I

H

H

R

E

K

K

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

D

T

T

Q

D

G

G

K

Q

V

V

D

D

I

V

L

F

V

I

L

A

G

G

V

V

G

G

T

T

G

G

S

T

I

L

T

T

G

G

V

V

G

S

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

H

G

N

K

P

T

N

D

I

L

K

I

I

S

I

V

A

A

V

V

E

E

P

P

E

T

N

D

S

S

P

P

V

V

-

I

-

A

-

Q

-

A

L

L

S

A

G

G

G

E

E

E

-

I

-

K

A

P

G

G

P

P

H

H

E

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

A

I

N

L

L

N

D

T

L

E

K

I

L

Y

V

D

D

E

K

I

V

I

I

R

G

V

I

S

T

G

N

E

E

D

E

A

A

I

I

N

S

C

T

S

A

R

R

E

R

M

L

A

M

K

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S

S

G

G

A

A

N

A

V

V

F

A

A

A

S

A

T

L

Q

K

A

L

A

Q

K

E

R

D

Q

E

E

S

N

F

R

T

G

N

K

K

T

N

I

I

V

V

T

V

I

I

L

L

C

P

D

S

T

S

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

A

L

L

Y

F

K42 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
49% identity, 95% coverage: 11:303/307 of query aligns to 13:313/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

I

L

N

N

I

R

A

I

S

G

Q

-

N

N

A

G

L

R

V

I

L

L

G

A

K

K

C

V

E

E

F

S

M

R

N

N

P

P

T

S

H

F

S

S

I

V

K
|
K

D

C

R

R

I

I

G

G

K

A

Y

N

M

M

I

I

Q

W

E

D

A

A

L

E

T

K

Q

R

G

G

L

V

I

L

T

K

K

P

N

G

T

V

T

E

V

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

Y

V

V

C

A

A

A

S

A

L

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

K

I

E

E

R

R

I

R

Q

K

L

L

R

K

A

A

L

L

G

G

A

A

R

N

V

L

V

V

L

L

T

T

D

E

A

G

K

A

Q

K

G

G

M

M

M

K

G

G

A

A

V

I

R

Q

E

K

A

A

K

E

E

E

L

I

-

V

C

A

S

S

Q

D

H

P

S

Q

E

K

A

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F
|
F

S

S

N

N

R

P

A

A

N

N

P

P

E

E

I

I

H

H

R

E

K

K

T

T

A

G

L

P

E

E

I

I

I

W

R

E

D

D

T

T

Q

D

G

G

K

Q

V

V

D

D

I

V

L

F

V

I

L

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

H

G

N

K

P

T

N

D

I

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

E

T

N

D

S

S

P

P

V

V

-

I

-

A

-

Q

-

A

L

L

S

A

G

G

G

E

E

E

-

I

-

K

A

P

G

G

P

P

H

H

E

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

G

I

N

L

L

N

D

T

L

E

K

I

L

Y

I

D

D

E

K

I

V

R

G

V

I

S

T

G

N

E

E

D

E

A

A

I

I

N

S

C

T

S

A

R

R

E

R

M

L

A

M

K

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

F

A

A

A

S

A

T

L

Q

K

A

L

A

Q

K

E

R

D

Q

E

E

S

N

F

R

T

G

N

K

K

T

N

I

I

V

V

T

V

I

I

L

L

C
x
P

D

S

T

S

A

G

E

E

R

R

Y

Y

L

L

S

S

C

T

G

A

L

L

Y

F

binding pyridoxal-5'-phosphate: K43 (= K42), N73 (= N72), V177 (= V175), G178 (= G176), T179 (= T177), G180 (= G178), T182 (≠ S180), G230 (= G220), S274 (= S264), P301 (≠ C291)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K42), T70 (= T69), G72 (= G71), N73 (= N72), T74 (= T73), Q144 (= Q142), F145 (= F143), Q229 (= Q219), G230 (= G220), I231 (= I221), A233 (= A223)

Query Sequence

>WP_011371790.1 NCBI__GCF_000012965.1:WP_011371790.1
MKYAKNVTELIGNTPLVRINIASQNALVLGKCEFMNPTHSIKDRIGKYMIQEALTQGLIT
KNTTVIEPTSGNTGIALASVCASLGIKLILTMPSSMSKERIQLLRALGARVVLTDAKQGM
MGAVREAKELCSQHSEALMLQQFSNRANPEIHRKTTALEIIRDTQGKVDILVLGVGTGGS
ITGVGEVLKKHNPNIKIIAVEPENSPVLSGGEAGPHEIQGIGAGFIPEILNTEIYDEIIR
VSGEDAINCSREMAKKEGLLVGISSGANVFASTQAAKRQENRGKTIVTILCDTAERYLSC
GLYDEDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory