SitesBLAST

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
26% identity, 91% coverage: 2:156/171 of query aligns to 3:158/173 of P10880

query
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P10880

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C13 (≠ V12) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K14) mutation to M: Loss of activity. Increased thermostability.
D34 (= D33) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
25% identity, 91% coverage: 2:156/171 of query aligns to 3:158/170 of 1e6cA

query
sites
1e6cA

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binding phosphate ion: R11 (≠ M10), G12 (= G11), M15 (≠ K14), T16 (≠ G15)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
25% identity, 91% coverage: 2:156/171 of query aligns to 3:148/162 of 2shkB

query
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2shkB

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binding adenosine-5'-diphosphate: G12 (= G11), C13 (≠ V12), G14 (= G13), K15 (= K14), T16 (≠ G15), T17 (≠ S16), R110 (= R107), Q145 (≠ D153), P147 (≠ S155)
binding magnesium ion: Q134 (≠ T142), A137 (= A145)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
27% identity, 96% coverage: 5:168/171 of query aligns to 5:166/179 of 2iyvA

query
sites
2iyvA

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binding adenosine-5'-diphosphate: G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R152 (≠ K154), P154 (≠ A156)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
27% identity, 96% coverage: 5:168/171 of query aligns to 5:166/168 of 1zyuA

query
sites
1zyuA

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binding phosphomethylphosphonic acid adenylate ester: P10 (≠ M10), G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), T149 (≠ V151), R152 (≠ K154), P154 (≠ A156)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D33), R57 (= R57), G80 (= G81), P117 (= P122), L118 (= L123), R135 (= R137)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
27% identity, 96% coverage: 5:168/171 of query aligns to 5:166/178 of 2iywA

query
sites
2iywA

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binding adenosine-5'-triphosphate: G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R152 (≠ K154), P154 (≠ A156)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
27% identity, 96% coverage: 5:168/171 of query aligns to 5:166/169 of 4bqsA

query
sites
4bqsA

V

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binding adenosine-5'-diphosphate: P10 (≠ M10), G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R152 (≠ K154), P154 (≠ A156)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D33), R135 (= R137)

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
27% identity, 96% coverage: 5:168/171 of query aligns to 5:166/169 of 2iyyA

query
sites
2iyyA

V

V

L

L

I

V

G

G

F

L

M

P

G

G

V

S

G

G

K
|
K

G

S

S

T

V

I

A

G

R

R

E

R

I

L

I

A

K

K

L

A

S

L

D

G

Y

V

M

G

S

L

V

L

D

D

T

T

D
|
D

D

V

L

A

I

I

E
|
E

S

Q

M

R

E

T

N

G

R

R

V
x
S

I
|
I

K

A

K

D

I

I

F

F

E

A

Q

T

S

D

G

G

E

E

A

Q

Y

E

F

F

R
|
R

N

R

L

I

E

E

K

E

R

D

-

V

V

V

S

R

L

A

W

A

L

L

G

A

Q

D

N

H

V

-

T

-

N

D

T

G

L

V

I

L

S

S

T

L

G

G

G
|
G

F

A

Y

V

K

T

Q

S

D

P

N

G

L

V

K

R

E

A

I

A

-

L

-

A

-

G

G

H

I

T

V

V

F

Y

L

L

N

E

S

I

P

S

F

A

E

A

K

E

I

G

L

V

K

R

R

R

I

T

K

G

N

G

H

-

P

-

N

N

A

T

V

V

K

-

L

-

K

-

R

R

P

P

L
|
L

L

L

K

A

D

G

L

P

K

D

K

R

A

A

-

E

-

K

-

Y

K

R

E

A

L

L

Y

M

Y

A

E

K

R
|
R

L

A

P

P

Q

L

Y

Y

T

R

A

R

V

V

A

A

D

T

I

M

T

R

I

V

D

D

V

T

T

N

D

R

K

R

S

N

A

P

L

G

D

A

C

V

A

V

K

R

E

H

L

I

L

L

L

S

K

R

V

L

K

Q

binding magnesium ion: E37 (= E37), S43 (≠ V43), I44 (= I44)
binding phosphate ion: K14 (= K14), G79 (= G80)
binding shikimate-3-phosphate: K14 (= K14), D33 (= D33), R57 (= R57), G79 (= G80), G80 (= G81), L118 (= L123), R135 (= R137)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
28% identity, 95% coverage: 5:167/171 of query aligns to 5:165/165 of 3bafA

query
sites
3bafA

V

V

L

L

I

V

G

G

F

L

M
x
P

G

G

V

S

G
|
G

K
|
K

G
x
S

S
x
T

V

I

A

G

R

R

E

R

I

L

I

A

K

K

L

A

S

L

D

G

Y

V

M

G

S

L

V

L

D

D

T

T

D
|
D

D

V

L

A

I

I

E

E

S

Q

M

R

E

T

N

G

R

R

V

S

I

I

K

A

K

D

I

I

F

F

E

A

Q

T

S

D

G

G

E

E

A

Q

Y

E

F

F

R

R

N

R

L

I

E

E

K

E

R

D

-

V

V

V

S

R

L

A

W

A

L

L

G

A

Q

D

N

H

V

-

T

-

N

D

T

G

L

V

I

L

S

S

T

L

G

G

F

A

Y

V

K

T

Q

S

D

P

N

G

L

V

K

R

E

A

I

A

-

L

-

A

-

G

G

H

I

T

V

V

F

Y

L

L

N

E

S

I

P

S

F

A

E

A

K

E

I

G

L

V

K

R

R
|
R

I

T

K

G

N

G

H

-

P

-

N

N

A

T

V

V

K

-

L

-

K

-

R
|
R

P

P

L

L

K

A

D

G

L

P

K

D

K

R

A

A

-

E

-

K

-

Y

K

R

E

A

L

L

Y

M

Y

A

E

K

R
|
R

L

A

P

P

Q

L

Y

Y

T

R

A

R

V

V

A

A

D

T

I

M

T

R

I

V

D

D

V

T

T

N

D

R

K

R

S

N

A

P

L

G

D

A

C

V

A

V

K

R

E

H

L

I

L

L

L

S

K

R

V

L

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ M10), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R116 (= R121)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D33), R135 (= R137)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
28% identity, 95% coverage: 5:167/171 of query aligns to 5:165/165 of 2dfnA

query
sites
2dfnA

V

V

L

L

I

V

G

G

F

L

M
x
P

G
|
G

V
x
S

G
|
G

K
|
K

G
x
S

S
x
T

V

I

A

G

R

R

E

R

I

L

I

A

K

K

L

A

S

L

D

G

Y

V

M

G

S

L

V

L

D

D

T

T

D
|
D

D

V

L

A

I

I

E

E

S

Q

M

R

E

T

N

G

R

R

V

S

I
|
I

K

A

K

D

I

I

F

F

E

A

Q

T

S

D

G

G

E

E

A

Q

Y

E

F
|
F

R
|
R

N

R

L

I

E

E

K

E

R

D

-

V

V

V

S

R

L

A

W

A

L

L

G

A

Q

D

N

H

V

-

T

-

N

D

T

G

L

V

I

L

S

S

T

L

G
|
G

F

A

Y

V

K

T

Q

S

D

P

N

G

L

V

K

R

E

A

I

A

-

L

-

A

-

G

G

H

I

T

V

V

F

Y

L

L

N

E

S

I

P

S

F

A

E

A

K

E

I

G

L

V

K

R

R
|
R

I

T

K

G

N

G

H

-

P

-

N

N

A

T

V

V

K

-

L

-

K

-

R
|
R

P

P

L

L

K

A

D

G

L

P

K

D

K

R

A

A

-

E

-

K

-

Y

K

R

E

A

L

L

Y

M

Y

A

E

K

R
|
R

L

A

P

P

Q

L

Y

Y

T

R

A

R

V

V

A

A

D

T

I

M

T

R

I

V

D

D

V

T

T

N

D

R

K
x
R

S

N

A
x
P

L

G

D

A

C

V

A

V

K

R

E

H

L

I

L

L

L

S

K

R

V

L

binding adenosine-5'-diphosphate: G11 (= G11), S12 (≠ V12), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R116 (= R121), R152 (≠ K154), P154 (≠ A156)
binding chloride ion: P10 (≠ M10), K14 (= K14), G79 (= G80)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D33), I44 (= I44), F56 (= F56), R57 (= R57), G78 (= G79), G79 (= G80), G80 (= G81), R135 (= R137)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
28% identity, 95% coverage: 5:167/171 of query aligns to 5:165/165 of 1we2A

query
sites
1we2A

V

V

L

L

I

V

G

G

F

L

M
x
P

G
|
G

V

S

G
|
G

K
|
K

G
x
S

S
x
T

V

I

A

G

R

R

E

R

I

L

I

A

K

K

L

A

S

L

D

G

Y

V

M

G

S

L

V

L

D

D

T

T

D
|
D

D

V

L

A

I

I

E

E

S

Q

M

R

E

T

N

G

R

R

V

S

I
|
I

K

A

K

D

I

I

F
|
F

E

A

Q

T

S

D

G

G

E

E

A

Q

Y

E

F

F

R

R

N

R

L

I

E

E

K

E

R

D

-

V

V

V

S

R

L

A

W

A

L

L

G

A

Q

D

N

H

V

-

T

-

N

D

T

G

L

V

I

L

S

S

T

L

G
|
G

F

A

Y

V

K

T

Q

S

D

P

N

G

L

V

K

R

E

A

I

A

-

L

-

A

-

G

G

H

I

T

V

V

F

Y

L

L

N

E

S

I

P

S

F

A

E

A

K

E

I

G

L

V

K

R

R
|
R

I

T

K

G

N

G

H

-

P

-

N

N

A

T

V

V

K

-

L

-

K

-

R
|
R

P

P

L
|
L

L

L

K

A

D

G

L

P

K

D

K

R

A

A

-

E

-

K

-

Y

K

R

E

A

L

L

Y

M

Y

A

E

K

R
|
R

L

A

P

P

Q

L

Y

Y

T

R

A

R

V

V

A

A

D

T

I

M

T

R

I

V

D

D

V

T

T

N

D

R

K

R

S

N

A

P

L

G

D

A

C

V

A

V

K

R

E

H

L

I

L

L

L

S

K

R

V

L

binding adenosine-5'-diphosphate: P10 (≠ M10), G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R116 (= R121)
binding 3-dehydroshikimate: D33 (= D33), I44 (= I44), F48 (= F48), G78 (= G79), G79 (= G80), G80 (= G81), L118 (= L123), R135 (= R137)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
26% identity, 95% coverage: 5:167/171 of query aligns to 5:163/163 of 1u8aA

query
sites
1u8aA

V

V

L

L

I

V

G

G

F

L

M

P

G
|
G

V

S

G
|
G

K
|
K

G
x
S

S
x
T

V

I

A

G

R

R

E

R

I

L

I

A

K

K

L

A

S

L

D

G

Y

V

M

G

S

L

V

L

D

D

T

T

D
|
D

D

V

L

A

I

I

E

E

S

Q

M

R

E

T

N

G

R

R

V

S

I

I

K

A

K

D

I

I

F
|
F

E

A

Q

T

S

D

G

G

E

E

A

Q

Y

E

F

F

R
|
R

N

R

L

I

E

E

K

E

R

D

-

V

V

V

S

R

L

A

W

A

L

L

G

A

Q

D

N

H

V

-

T

-

N

D

T

G

L

V

I

L

S

S

T

L

G

G

G
|
G

F

A

Y

V

K

T

Q

S

D

P

N

G

L

V

K

R

E

A

I

A

-

L

-

A

-

G

G

H

I

T

V

V

F

Y

L

L

N

E

S

I

P

S

F

A

E

A

K

E

I

G

L

V

K

R

R
|
R

I

T

K

G

N

G

H

-

P

-

N

-

A

-

V

-

K

-

L

-

K

-

K

V

R

R

P

P

L
|
L

L

L

K

A

D

G

L

P

K

D

K

R

A

A

-

E

-

K

-

Y

K

R

E

A

L

L

Y

M

Y

A

E

K

R
|
R

L

A

P

P

Q

L

Y

Y

T

R

A

R

V

V

A

A

D

T

I

M

T

R

I

V

D

D

V

T

T

N

D

R

K
x
R

S

N

A
x
P

L

G

D

A

C

V

A

V

K

R

E

H

L

I

L

L

L

S

K

R

V

L

binding adenosine-5'-diphosphate: G11 (= G11), G13 (= G13), K14 (= K14), S15 (≠ G15), T16 (≠ S16), R109 (= R107), R150 (≠ K154), P152 (≠ A156)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D33), F48 (= F48), R57 (= R57), G79 (= G80), G80 (= G81), L116 (= L123), R133 (= R137)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
24% identity, 91% coverage: 2:156/171 of query aligns to 3:143/158 of 1shkA

query
sites
1shkA

K

E

N

P

I

I

V

F

L

M

I

V

G

G

F

A

M

R

G

G

V

C

G

G

K

K

G

T

S

T

V

V

A

G

R

R

E

E

I

L

I

A

K

R

L

A

S

L

D

G

Y

Y

M

E

S

F

V

V

D

D

T

T

D

D

D

I

L

F

I

M

E

Q

S

H

M

T

E

S

N

G

R

M

V

T

I

V

K

A

K

D

I

V

F

V

E

A

Q

A

S

E

G

G

E

W

A

P

Y

G

F

F

R

R

N

R

L

R

E

E

K

S

R

E

V

A

S

L

L

Q

W

A

L

V

G

A

Q

-

N

-

V

T

T

P

N

N

T

R

L

V

I

V

S

A

T

T

G

G

F

M

Y

V

-

L

-

E

-

Q

K

N

Q

R

D

Q

N

F

L

M

K
x
R

E
x
A

I

H

G
|
G

I

T

V

V

F

Y

L

L

N

F

S

A

P

P

F

A

E

E

K

E

I

L

L

A

K

L

R

R

I

L

K

Q

N

I

H

A

P

E

N

E

A

M

V

E

K

A

K

V

L

L

K

R

K

E

R

R

P

E

L

A

L

L

K

-

D

-

L

-

K

-

A

-

K

-

E

-

L

-

Y

-

E

-

R

-

L

-

P

-

Q

-

Y

Y

T
x
Q

A

D

V

V

A
|
A

D

H

I

Y

T

V

I

V

D

D

V

A

T

T

D

Q

K

P

S

P

A

A

binding magnesium ion: R92 (≠ K89), A93 (≠ E90), G95 (= G92), Q129 (≠ T142), A132 (= A145)

Query Sequence

>WP_011372129.1 NCBI__GCF_000012965.1:WP_011372129.1
MKNIVLIGFMGVGKGSVAREIIKLSDYMSVDTDDLIESMENRVIKKIFEQSGEAYFRNLE
KRVSLWLGQNVTNTLISTGGGFYKQDNLKEIGIVVFLNSPFEKILKRIKNHPNAVKKLKK
RPLLKDLKKAKELYYERLPQYTAVADITIDVTDKSALDCAKELLLKVKQYA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory