SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011372739.1 NCBI__GCF_000012965.1:WP_011372739.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
54% identity, 99% coverage: 3:427/430 of query aligns to 1:426/426 of P23893

query
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P23893

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K265 (= K266) mutation to R: 2% of wild-type activity.

3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
53% identity, 100% coverage: 2:429/430 of query aligns to 3:429/430 of 3bs8A

query
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3bs8A

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active site: V22 (= V21), Y145 (= Y144), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), G401 (≠ T401)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G117), T119 (= T118), Y145 (= Y144), H146 (= H145), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)

P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
52% identity, 99% coverage: 2:427/430 of query aligns to 49:474/474 of P42799

query
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P42799

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I

H

T

T

D

P

E

E

M

D

I

I

E

Q

D

L

T

T

I

I

K

A

A

A

S

A

A

E

K

R

V

V

F

L

K

S

E

R

I

I

K314 (= K266) modified: N6-(pyridoxal phosphate)lysine

5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 99% coverage: 2:427/430 of query aligns to 3:428/428 of 5hdmB

query
sites
5hdmB

S

S

I

L

D

Q

N

K

S

S

L

E

K

E

A

A

F

F

K

N

E

A

A

A

Q

K

N

N

L

L

I

M

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

Q

P

P

L

V

F

L

I

I

A

D

N

S

G

V

S

K

G

G

A

S

Y

K

L

M

T

W

D

D

I

I

D

D

G

G

N

N

R

E

Y

Y

V

I

D

D

F

Y

V

V

Q

G

S

S

W

W

G

G

P

P

L

A

L

I

F

I

G

G

H

H

R

A

D

D

E

D

S

E

I

V

E

L

S

A

A

A

V

L

I

A

E

E

A

T

V

M

K

K

H

K

G

G

L

T

S

S

F

F

G

G

A

A

P

P

T

C

Q

L

A

L

E

E

S

N

D

V

L

L

A

A

A

E

L

M

V

V

I

I

S

S

M

A

F

V

D

P

S

S

I

I

E

E

K

M

I

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

V

C

M

M

S

G

A

V

I

L

R

R

L

L

A

A

R

R

G

A

Y

F

T

T

N

N

C

K

D

E

D

K

I

F

V

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

N

S

A

L

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

A

V

A

A

T

T

F

L

G

G

N

L

P

P

S

D

S

S

P

P

G

G

V

V

P

P

A

K

D

A

F

A

T

T

K

S

H

D

T

T

L

L

L

T

A

A

E

P

Y

Y

N

N

N

D

I

L

E

E

S

A

V

V

K

E

K

K

C

L

F

F

S

A

D

A

S

H

K

K

-

G

D

E

V

I

A

S

C

A

V

V

I

I

I

L

E
|
E

P

P

I

V

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

P

A

P

D

T

K

P

E

E

F

F

L

I

R

N

E

G

L

L

R

R

E

Q

L

L

C

T

D

K

A

D

N

N

G

G

A

V

L

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

S

T

G

G

F

F

R

R

A

L

S

A

V

Y

H

G

G

G

A

A

E

Q

S

E

I

Y

T

F

G

G

V

I

K

T

P

P

D

D

I

L

V

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

R

A

R

E

D

I

I

M

M

A

E

K

M

L

V

S

A

P

P

E

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

T

A

A

G

G

L

I

A

H

A

T

I

L

T

K

K

R

L

L

K

K

Q

Q

N

A

G

G

Q

T

I

-

I

Y

S

E

V

Y

L

L

N

D

S

K

R

I

A

T

T

K

R

E

L

L

V

T

E

N

G

G

M

I

Q

L

E

E

A

A

A

G

K

K

T

K

Y

T

G

G

I

H

A

P

M

M

Q

C

I

G

D

G

T

Y

R

I

G

S

S

G

M

M

F

F

G

G

F

F

F

F

F

F

N

A

E

E

K

G

P

P

V

V

K

Y

N

N

F

F

A

A

D

D

A

S

C

K

N

K

S

S

D

D

E

T

K

E

L

K

F

F

A

G

L

R

F

F

H

F

S

R

K

G

M

M

L

L

K

E

E

E

G

G

F

V

Y

Y

F

F

A

A

C

P

S

S

L

Q

Y

F

E

E

T
x
A

G

G

F

F

I

T

S

S

T

L

A

A

I

H

T

T

D

P

E

E

M

D

I

I

E

Q

D

L

T

T

I

I

K

A

A

A

S

A

A

E

K

R

V

V

F

L

K

S

E

R

I

I

active site: V22 (= V21), Y145 (= Y144), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (≠ T401)
binding pyridoxal-5'-phosphate: G118 (= G117), T119 (= T118), Y145 (= Y144), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G117), T119 (= T118), Y145 (= Y144), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)

5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 99% coverage: 2:427/430 of query aligns to 3:428/428 of 5hdmA

query
sites
5hdmA

S

S

I

L

D

Q

N

K

S

S

L

E

K

E

A

A

F

F

K

N

E

A

A

A

Q

K

N

N

L

L

I

M

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

Q

P

P

L

V

F

L

I

I

A

D

N

S

G

V

S

K

G

G

A

S

Y

K

L

M

T

W

D

D

I

I

D

D

G

G

N

N

R

E

Y

Y

V

I

D

D

F

Y

V

V

Q

G

S

S

W

W

G

G

P

P

L

A

L

I

F

I

G

G

H

H

R

A

D

D

E

D

S

E

I

V

E

L

S

A

A

A

V

L

I

A

E

E

A

T

V

M

K

K

H

K

G

G

L

T

S

S

F

F

G

G

A

A

P

P

T

C

Q

L

A

L

E

E

S

N

D

V

L

L

A

A

A

E

L

M

V

V

I

I

S

S

M

A

F

V

D

P

S

S

I

I

E

E

K

M

I

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

V

C

M

M

S

G

A

V

I

L

R

R

L

L

A

A

R

R

G

A

Y

F

T

T

N

N

C

K

D

E

D

K

I

F

V

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G
|
G

H

H

S

A

D

N

S

A

L

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

A

V

A

A

T

T

F

L

G

G

N

L

P

P

S

D

S

S

P

P

G

G

V

V

P

P

A

K

D

A

F

A

T

T

K

S

H

D

T

T

L

L

L

T

A

A

E

P

Y

Y

N

N

N

D

I

L

E

E

S

A

V

V

K

E

K

K

C

L

F

F

S

A

D

A

S

H

K

K

-

G

D

E

V

I

A

S

C

A

V

V

I

I

I

L

E
|
E

P

P

I

V

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

P

A

P

D

T

K

P

E

E

F

F

L

I

R

N

E

G

L

L

R

R

E

Q

L

L

C

T

D

K

A

D

N

N

G

G

A

V

L

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

S

T

G

G

F

F

R

R

A

L

S

A

V

Y

H

G

G

G

A

A

E

Q

S

E

I

Y

T

F

G

G

V

I

K

T

P

P

D

D

I

L

V

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

R

A

R

E

D

I

I

M

M

A

E

K

M

L

V

S

A

P

P

E

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

T

A

A

G

G

L

I

A

H

A

T

I

L

T

K

K

R

L

L

K

K

Q

Q

N

A

G

G

Q

T

I

-

I

Y

S

E

V

Y

L

L

N

D

S

K

R

I

A

T

T

K

R

E

L

L

V

T

E

N

G

G

M

I

Q

L

E

E

A

A

A

G

K

K

T

K

Y

T

G

G

I

H

A

P

M

M

Q

C

I

G

D

G

T

Y

R

I

G

S

S

G

M

M

F

F

G

G

F

F

F

F

F

F

N

A

E

E

K

G

P

P

V

V

K

Y

N

N

F

F

A

A

D

D

A

S

C

K

N

K

S

S

D

D

E

T

K

E

L

K

F

F

A

G

L

R

F

F

H

F

S

R

K

G

M

M

L

L

K

E

E

E

G

G

F

V

Y

Y

F

F

A

A

C

P

S

S

L

Q

Y

F

E

E

T
x
A

G

G

F

F

I

T

S

S

T

L

A

A

I

H

T

T

D

P

E

E

M

D

I

I

E

Q

D

L

T

T

I

I

K

A

A

A

S

A

A

E

K

R

V

V

F

L

K

S

E

R

I

I

active site: V22 (= V21), Y145 (= Y144), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (≠ T401)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G117), T119 (= T118), Y145 (= Y144), G147 (= G146), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)

5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
54% identity, 98% coverage: 3:424/430 of query aligns to 1:418/420 of 5i92F

query
sites
5i92F

I

M

D

S

N

R

S

S

L

E

K

T

A

L

F

F

K

N

E

N

A

A

Q

Q

N

K

L

H

I

I

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

T

P

P

L

L

F

F

I

F

A

K

N

H

G

A

S

E

G

G

A

A

Y

Y

L

V

T

L

D

D

I

E

D

D

G

D

N

K

R

R

Y

Y

V

V

D

D

F

Y

V

V

Q

G

S

S

W

W

G

G

P

P

L

M

L

I

F

L

G

G

H

H

R

S

D

H

E

P

S

D

I

V

E

L

S

D

A

A

V

V

I

R

E

R

A

Q

V

L

K

D

H

H

G

G

L

L

S

S

F

Y

G

G

A

A

P

P

T

T

Q

A

A

L

E

E

S

V

D

E

L

M

A

A

A

D

L

L

V

V

I

C

S

S

M

M

F

V

D

P

S

S

I

M

E

E

K

M

I

V

R

R

F

M

V

V

S

S

S

S

G

G

T

T

E

E

A

A

V

T

M

M

S

S

A

A

I

I

R

R

L

L

A

A

R

R

G

G

Y

Y

T

T

N

G

C

R

D

D

D

S

I

I

V

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

S

D

D

S

S

L

L

L

L

V

V

Q

K

A

A

G

G

S

S

G

-

A

-

A

-

T

T

F

F

G

G

N

V

P

P

S

N

S

S

P

P

G

G

V

V

P

P

A

A

D

A

F

F

T

A

K

K

H

H

T

T

L

L

L

T

A

L

E

P

Y

F

N

N

N

D

I

I

E

E

S

A

V

V

K

R

K

K

C

T

F

L

S

G

D

E

-

V

S

G

K

K

D

E

V

V

A

A

C

C

V

I

I

I

I

V

E
|
E

P

P

I

V

A

A

G

G

N

N

M

M

G

N

L

C

V

V

P

P

A

P

D

A

K

P

E

G

F

F

L

L

R

E

E

G

L

L

R

R

E

E

L

A

C

C

D

D

A

E

N

H

G

G

A

V

L

V

L

L

I

I

F

F

D
|
D

E

E

V

V

M
|
M

S

T

G

G

F

F

R

R

A

V

S

A

V

L

H

G

G

G

A

A

E

Q

S

A

I

Y

T

Y

G

G

V

V

K

T

P

P

D

D

I

L

V

S

T

T

L

F

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

M

P

P

V

V

G

G

A

A

F

F

G

G

A

G

R

K

A

R

E

E

I

I

M

M

A

Q

K

Q

L

I

S

S

P

P

E

L

G

G

P

P

V

V

Y

Y

Q

Q

A

A

G

G

T

T

L

-

S

-

G

G

N

N

P

P

V

L

A

A

M

M

A

A

A

A

G

G

L

L

A

T

A

T

I

L

T

R

K

L

L

I

K

S

Q

R

N

P

G

G

Q

-

I

F

I

H

S

D

V

E

L

L

N

T

S

A

R

Y

A

T

T

T

R

R

L

M

V

L

E

D

G

G

M

L

Q

Q

E

Q

A

R

A

A

K

D

T

A

Y

A

G

G

I

I

A

P

M

F

Q

V

I

T

D

T

T

Q

R

A

G

G

S

G

M

M

F

F

G

G

F

L

F

Y

F

F

N

S

-

G

E

A

K

D

P

A

V

I

K

V

N

T

F

F

A

E

D

D

A

V

C

M

N

A

S

S

D

D

E

V

K

E

L

R

F

F

A

K

L

R

F

F

H

F

S

H

K

L

M

M

L

L

K

D

E

G

G

G

F

V

Y

Y

F

L

A

A

C

P

S

S

L

A

Y

F

E

E

T
x
A

G

G

F

F

I

T

S

S

T

I

A

A

I

H

T

G

D

D

E

K

M

E

I

L

E

E

D

I

T

T

I

L

K

N

A

A

S

A

A

E

K

K

V
x
A

F
|
F

active site: V19 (= V21), Y142 (= Y144), E201 (= E205), D234 (= D238), M237 (= M241), K262 (= K266), A395 (≠ T401)
binding leucine: A417 (≠ V423), F418 (= F424)

Sites not aligning to the query:

binding leucine: 420

3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
52% identity, 95% coverage: 4:410/430 of query aligns to 5:405/423 of 3k28A

query
sites
3k28A

D

D

N

K

S

S

L

I

K

A

A

A

F

F

K

E

E

E

A

A

Q

Q

N

D

L

L

I

M

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

M

T

N

P

P

L

L

F

F

I

M

A

E

N

R

G

G

S

K

G

G

A

S

Y

K

L

V

T

Y

D

D

I

I

D

D

G

G

N

N

R

E

Y

Y

V

I

D

D

F

Y

V

V

Q

L

S

S

W

W

G

G

P

P

L

L

L

I

F

H

G

G

H

H

R

A

D

N

E

D

S

R

I

V

E

V

S

E

A

A

V

L

I

K

E

A

A

V

V

A

K

E

H

R

G

G

L

T

S

S

F

F

G

G

A

A

P

P

T

T

Q

E

A

I

E

E

S

N

D

K

L

L

A

A

A

K

L

L

V

V

I
|
I

S

E

M

R

F
x
V

D
x
P

S

S

I
|
I

E

E

K

I

I

V

R

R

F

M

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

V

T

M

M

S

S

A

A

I

L

R

R

L

L

A

A

R

R

G

G

Y

Y

T

T

N

G

C

R

D

N

D

K

I

I

V

L

K

K

F

F

T

I

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

S

G

D

D

S

S

L

L

L

L

V

I

Q

K

A

A

G

G

S

S

G

G

A

V

A

-

T

-

F

-

G

-

N

-

P

P

S

D

S

S

P

P

G

G

V

V

P

P

A

E

D

G

F

V

T

A

K

K

H

N

T

T

L

I

L

T

A

V

E

A

Y

Y

N

N

N

D

I

L

E

E

S

S

V

V

K

K

K

Y

C

A

F

F

S

E

D

Q

-

F

S

G

K

D

D

D

V

I

A

A

C

C

V

V

I

I

I

V

E
|
E

P

P

I

V

A

A

G

G

N

N

M

M

G

G

L

V

V

V

P

P

A

P

D

Q

K

P

E

G

F

F

L

L

R

E

E

G

L

L

R

R

E

E

L

V

C

T

D

E

A

Q

N

N

G

G

A

A

L

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

S

T

G

G

F

F

R

R

A

V

S

A

V

Y

H

N

G

C

A

G

E

Q

S

G

I

Y

T

Y

G

G

V

V

K

T

P

P

D

D

I

L

V

T

T

C

L

L

G

G

K
|
K

V

V

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

K

A

A

E

E

I

I

M

M

A

R

K

Q

L

V

S

A

P

P

E

S

G

G

P

P

V

I

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

A

A

A

G

G

L

Y

A

E

A

T

I

L

T

V

K

Q

L

L

K

T

Q

P

N

E

G

S

Q

Y

I

V

I

-

S

-

V

E

L

F

N

E

S

R

R

K

A

A

T

E

R

M

L

L

V

E

E

A

G

G

M

L

Q

R

E

K

A

A

A

A

K

E

T

K

Y

H

G

G

I

I

A

P

M

H

Q

H

I

I

D

N

T

R

R

A

G

G

S

S

M

M

F

I

G

G

F

I

F

F

F

F

N

T

E

D

K

E

P

P

V

V

K

I

N

N

F

Y

A

D

D

A

A

A

C

K

N

S

S

S

D

N

E

L

K

Q

L

F

F

F

A

A

L

A

F

Y

H

Y

S

R

K

E

M

M

L

V

K

E

E

Q

G

G

F

V

Y

F

F

L

A

P

C

P

S

S

L

Q

Y

F

E

E

T
x
G

G

L

F

F

I

L

S

S

T

T

A

V

I

H

T

S

D

D

active site: V22 (= V21), Y145 (= Y144), E202 (= E205), D235 (= D238), M238 (= M241), K263 (= K266), G396 (≠ T401)
binding calcium ion: I103 (= I102), V106 (≠ F105), P107 (≠ D106), I109 (= I108)
binding pyridoxal-5'-phosphate: G118 (= G117), T119 (= T118), Y145 (= Y144), H146 (= H145), G147 (= G146), E202 (= E205), D235 (= D238), V237 (= V240), M238 (= M241), K263 (= K266)

Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
51% identity, 99% coverage: 2:427/430 of query aligns to 47:472/472 of Q42522

query
sites
Q42522

S

T

I

L

D

Q

N

K

S

S

L

E

K

E

A

A

F

F

K

N

E

A

A

A

Q

K

N

N

L

L

I

M

P

P

G

G

G

G

V

V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

Q

P

P

L

V

F

V

I

M

A

D

N

S

G

A

S

K

G

G

A

S

Y

R

L

I

T
x
R

D

D

I

I

D

D

G

G

N

N

R

E

Y

Y

V

I

D

D

F

Y

V

V

Q

G

S

S

W

W

G

G

P

P

L

A

L

I

F

I

G

G

H

H

R

A

D

D

E

D

S

E

I

V

E

L

S

A

A

A

V

L

I

A

E

E

A

T

V

M

K

K

H

K

G

G

L

T

S

S

F

F

G

G

A

A

P

P

T

C

Q

L

A

L

E

E

S

N

D

V

L

L

A

A

A

E

L

M

V

V

I

I

S

S

M

A

F

V

D

P

S

S

I

I

E

E

K

M

I

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T

T

E

E

A

A

V

C

M

M

S

G

A

V

I

L

R

R

L

L

A

A

R

R

G

A

Y

F

T

T

N

G

C

K

D

Q

D

K

I

F

V

I

K

K

F

F

T

E

G

G

C

C

Y

Y

H

H

G

G

H

H

S

A

D

N

S

S

L

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

A

V

A

A

T

T

F

L

G

G

N

L

P

P

S

D

S

S

P

P

G

G

V

V

P

P

A

K

D

A

F

A

T

T

K

S

H

D

T

T

L

L

L

T

A

A

E

P

Y

Y

N

N

N

D

I

I

E

A

S

A

V

V

K

E

K

K

C

L

F

F

S

E

D

A

S

N

K

K

-

G

D

E

V

I

A

A

C

A

V

I

I

I

I

L

E

E

P

P

I

V

A

V

G

G

N

N

M

S

G

G

L

F

V

I

P

T

A

P

D

K

K

P

E

E

F

F

L

I

R

E

E

G

L

I

R

R

E

R

L

I

C

T

D

K

A

D

N

N

G

G

A

A

L

L

L

L

I

I

F

F

D

D

E

E

V

V

M

M

S

T

G

G

F

F

R

R

A

L

S

A

V

Y

H

G

G

G

A

A

E

Q

S

E

I

Y

T

F

G

G

V

I

K

T

P

P

D

D

I

L

V

T

T

T

L

L

G

G

K

K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

R

A

R

E

D

I

I

M

M

A

E

K

M

L

V

S

A

P

P

E

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

T

A

A

G

G

L

I

A

H

A

T

I

L

T

K

K

R

L

L

K

S

Q

Q

N

P

G

G

Q

T

I

-

I

Y

S

E

V

Y

L

L

N

D

S

K

R

I

A

T

T

K

R

E

L

L

V

T

E

N

G

G

M

I

Q

L

E

E

A

A

A

G

K

K

T

K

Y

T

G

G

I

H

A

A

M

M

Q

C

I

G

D

G

T

Y

R

I

G

S

S

G

M

M

F

F

G

G

F

F

F

F

F

F

N

T

E

E

K

G

P

P

V

V

K

Y

N

D

F

F

A

S

D

D

A

A

C

K

N

K

S

S

D

D

E

T

K

E

L

K

F

F

A

G

L

K

F

F

H

F

S

R

K

G

M

M

L

L

K

E

E

E

G

G

F

V

Y

Y

F

L

A

A

C

P

S

S

L

Q

Y

F

E

E

T

A

G

G

F

F

I

T

S

S

T

L

A

A

I

H

T

T

D

S

E

E

M

D

I

I

E

Q

D

F

T

T

I

I

K

A

A

A

S

A

A

E

K

K

V

V

F

L

K

S

E

R

I

L

R92 (≠ T47) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
G162 (= G117) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.

6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
50% identity, 97% coverage: 1:417/430 of query aligns to 6:419/430 of 6w80A

query
sites
6w80A

M

M

S

N

I

H

D

D

N

Q

S

S

L

H

K

A

A

L

F

F

K

S

E

R

A

A

Q

Q

N

Q

L

L

I

L

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

E

P

P

L

F

F

F

I

V

A

E

N

R

G

A

S

D

G

G

A

A

Y

Y

L

L

T

Y

D

D

I

V

D

D

G

G

N

N

R

R

Y

Y

V

I

D

D

F

Y

V

V

Q

G

S

S

W

W

G

G

P

P

L

M

L

I

F

V

G

G

H

H

R

N

D

H

E

P

S

A

I

V

E

R

S

Q

A

A

V

V

I

K

E

K

A

A

V

I

K

D

H

N

G

G

L

L

S

S

F

F

G

G

A

A

P

P

T

C

Q

A

A

G

E

E

S

V

D

T

L

M

A

A

A

E

L

T

V

I

I

T

S

R

M

L

F

V

D

P

S

S

I

C

E

E

K

M

I

V

R

R

F

M

V

V

S

N

S
|
S

G
|
G

T
|
T

E

E

A

A

V

T

M

L

S

S

A

A

I

I

R

R

L

L

A

A

R

R

G

G

Y

A

T

T

N

G

C

R

D

N

D

R

I

I

V

V

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

S

G

D

D

S

S

L

F

L

L

V

V

Q

K

A

A

G

-

S

-

G

-

A

M

A

L

T

T

F

L

G

G

N

V

P

P

S

T

S

S

P

P

G

G

V

V

P

P

A

A

D

G

F

L

T

S

K

E

H

L

T

T

L

L

L

T

A

L

E

P

Y

Y

N

N

N

D

I

F

E

E

S

A

V

A

K

T

K

A

C

L

F

F

S

E

D

Q

S

Q

-

G

K

D

D

D

V

I

A

A

C

G

V

L

I

I

I

I

E
|
E

P

P

I

V

A

V

G

G

N
|
N

M

A

G

N

L

C

V

I

P

P

A

P

D

R

K

E

E

G

F

Y

L

L

R

Q

E

H

L

L

R

R

E

A

L

L

C

C

D

T

A

K

N

H

G

G

A

A

L

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M

M

S

T

G

G

F

F

R

R

A

V

S

A

V

L

H

G

G

G

A

A

E

Q

S

A

I

H

T

Y

G

G

V

I

K

T

P

P

D

D

I

L

V

T

T

T

L

F

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

M

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

R

A

R

E

E

I

L

M

M

A

Q

K

Q

L

I

S

A

P

P

E

A

G

G

P

P

V

I

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

V

A

A

M

M

A

A

A

A

G

G

L

L

A

A

A

M

I

L

T

E

K

L

L

V

K

Q

Q

Q

N

P

G

G

Q

-

I

F

I

H

S

A

V

D

L

L

N

A

S

E

R

R

A

T

T

A

R

R

L

L

V

C

E

A

G

G

M

L

Q

E

E

A

A

A

A

A

K

A

T

D

Y

A

G

G

I

V

A

A

M

V

Q

T

I

T

D

T

T

R

R

V

G

G

S

A

M

M

F

F

G

G

F

L

F

F

F

F

N

T

E

S

K

E

P

K

V

V

K

E

N

T

F

Y

A

A

D

Q

A

A

C

T

N

A

S

C

D

D

E

I

K

P

L

A

F

F

A

N

L

R

F

F

H

F

S

H

K

A

M

M

L

L

K

E

E

Q

G

G

F

V

Y

F

F

L

A

A

C

P

S

S

L

A

Y

Y

E

E

T

A

G

G

F

F

I

L

S

S

T

S

A

A

I

H

T

D

D

D

E

A

M

V

I

I

E

E

D

A

T

T

I

L

active site: V26 (= V21), Y149 (= Y144), D241 (= D238), K269 (= K266)
binding pyridoxal-5'-phosphate: S121 (= S116), G122 (= G117), T123 (= T118), Y149 (= Y144), H150 (= H145), E208 (= E205), N213 (= N210), D241 (= D238), V243 (= V240), K269 (= K266)

2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
49% identity, 97% coverage: 10:424/430 of query aligns to 10:424/424 of 2e7uA

query
sites
2e7uA

F

F

K

Q

E

E

A

A

Q

K

N

R

L

H

I

I

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

A

V

V

G

G

G

G

T

T

P

P

L

P

F

F

I

L

A

V

N

R

G

G

S

E

G

G

A

A

Y

Y

L

V

T

W

D

D

I

A

D

D

G

G

N

N

R

R

Y

Y

V

L

D

D

F

Y

V

V

Q

M

S

S

W

W

G

G

P

P

L

L

L

I

F

L

G

G

H

H

R

A

D

H

E

P

S

K

I

V

E

L

S

A

A

R

V

V

I

R

E

E

A

T

V

L

K

E

H

R

G

G

L

L

S

T

F

F

G

G

A

A

P

P

T

S

Q

P

A

L

E

E

S

V

D

A

L

L

A

A

A

K

L

K

V

V

I

K

S

R

M

A

F

Y

D

P

S

F

I

V

E

D

K

L

I

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

V

T

M

M

S

S

A

A

I

L

R

R

L

L

A

A

R

R

G

G

Y

Y

T

T

N

G

C

R

D

P

D

Y

I

I

V

V

K

K

F

F

T

R

G

G

C

N

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

G

L

L

L

L

V

V

Q

E

A

A

G

G

S

S

G

G

A

A

A

L

T

T

F

L

G

G

N

V

P

P

S

S

S

S

P

A

G

G

V

V

P

P

A

E

D

E

F

Y

T

A

K

K

H

L

T

T

L

L

L

V

A

L

E

E

Y

Y

N

N

N

D

I

P

E

E

S

G

V

L

K

R

K

E

C

V

F

L

S

K

D

R

S

R

-

G

K

E

D

E

V

I

A

A

C

A

V

I

I

I

I

F

E
|
E

P

P

I

V

A

V

G

G

N
|
N

M

A

G

G

L

V

V

L

P

V

A

P

D

T

K

E

E

D

F

F

L

L

R

K

E

A

L

L

R

H

E

E

L

-

C

A

D

K

A

A

N

Y

G

G

A

V

L

L

L

L

I

I

F

A

D
|
D

E

E

V
|
V

M
|
M

S

T

G

G

F

F

R

R

A

L

S

A

V

F

H

G

G

G

A

A

E

T

S

E

I

L

T

L

G

G

V

L

K

K

P

P

D

D

I

L

V

V

T

T

L

L

G

G

K
|
K

V

I

I

L

G

G

G

G

G

G

M

L

P

P

V

A

G

A

A

A

F

Y

G

A

A

G

R

R

A

R

E

E

I

I

M

M

A

E

K

K

L

V

S

A

P

P

E

L

G

G

P

P

V

V

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

A

A

A

G

G

L

L

A

A

A

T

I

L

T

E

K

L

L

L

K

E

Q

E

N

N

G

P

Q

G

I

Y

I

Y

S

A

V

Y

L

L

N

E

S

D

R

L

A

G

T

A

R

R

L

L

V

E

E

A

G

G

M

L

Q

K

E

E

A

V

A

L

K

K

T

E

Y

K

G

G

I

L

A

P

M

H

Q

T

I

V

D

N

T

R

R

V

G

G

S

S

M

M

F

I

G

T

F

V

F

F

F

F

N

T

E

E

K

G

P

P

V

V

K

V

N

T

F

F

A

Q

D

D

A

A

C

R

N

R

S

T

D

D

E

T

K

E

L

L

F

F

A

K

L

R

F

F

H

F

S

H

K

G

M

L

L

L

K

D

E

R

G

G

F

I

Y

Y

F

W

A

P

C

P

S

S

L

N

Y

F

E

E

T
x
A

G

A

F

F

I

L

S

S

T

V

A

A

I

H

T

R

D

E

E

E

M

D

I

V

E

E

D

K

T

T

I

L

K

E

A

A

S

L

A

R

K

K

V

A

F

L

active site: V21 (= V21), Y144 (= Y144), E206 (= E205), D238 (= D238), M241 (= M241), K266 (= K266), A401 (≠ T401)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G117), T118 (= T118), Y144 (= Y144), H145 (= H145), N211 (= N210), D238 (= D238), V240 (= V240)

2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
50% identity, 97% coverage: 10:427/430 of query aligns to 10:427/427 of 2gsaB

query
sites
2gsaB

F

F

K

A

E

A

A

A

Q

Q

N

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

T

Q

P

P

L

I

F

V

I

F

A

D

N

R

G

V

S

K

G

D

A

A

Y

Y

L

A

T

W

D

D

I

V

D

D

G

G

N

N

R

R

Y

Y

V

I

D

D

F

Y

V

V

Q

G

S

T

W

W

G

G

P

P

L

A

L

I

F

C

G

G

H

H

R

A

D

H

E

P

S

E

I

V

E

I

S

E

A

A

V

L

I

K

E

V

A

A

V

M

K

E

H

K

G

G

L

T

S

S

F

F

G

G

A

A

P

P

T

C

Q

A

A

L

E

E

S

N

D

V

L

L

A

A

A

E

L

M

V

V

I

N

S

D

M

A

F

V

D

P

S

S

I

I

E

E

K

M

I

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

V

C

M

M

S

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

Y

Y

T

T

N

G

C

R

D

D

D

K

I

I

V

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

A

V

A

A

T

T

F

L

G

G

N

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

A

K

D

K

F

T

T

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

Y

Y

N

N

N

D

I

L

E

E

S

A

V

V

K

K

K

A

C

L

F

F

S

A

D

E

S

N

K

P

-

G

D

E

V

I

A

A

C

G

V

V

I

I

I

L

E
|
E

P

P

I

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

A

P

D

D

K

A

E

G

F

F

L

L

R

E

E

G

L

L

R

R

E

E

L

I

C

T

D

L

A

E

N

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

S

T

G

G

F

F

R

R

A

I

S

A

V

Y

H

G

G

G

A

V

E

Q

S

E

I

K

T

F

G

G

V

V

K

T

P

P

D

D

I

L

V

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

F

Y

G

G

A

G

R

K

A

R

E

E

I

I

M

M

A

Q

K

L

L

V

S

A

P

P

E

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

V

L

A

A

M

M

A

T

A

A

G

G

L

I

A

K

A

T

I

L

T

E

K

L

L

L

K

R

Q

Q

N