SitesBLAST

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
39% identity, 75% coverage: 20:138/159 of query aligns to 7:135/145 of 7s42A

query
sites
7s42A

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

S

N

I

I

G

G

D

S

E

G

T

T

K

R

I

I

G

W

A

Q

F

F

V

C

E

V

I

V

Q

L

G

P

G

S

A

A

T

I

I

I

G

G

A

E

R

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
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F

V

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

-

C

-

G

-

V

-

Q

-

I

C
x
W

E

D

G

G

V

I

T

E

I

I

E

E

D

D

E

D

V

V

F

F

V

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
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N

D
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D

V
x
K

Y
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Y

P
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P

R

R

A

S

T

K

T

Q

P
x
Y

D

P

G

K

A

E

L

F

A

-

T

-

A

-

D

-

W

-

T

-

C

-

S

S

P

K

T

T

V

I

K

K

R

K

R

G

A

A

S

S

I

I

G

G

S

A

G

N

A

A

T

T

I

I

L
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L

P

P

N

G

L

I

T

T

I

I

G

G

E

E

N

N

A

A

L

M

V

I

A

G

A

A

G

G

A

A

V

I

V

V

T
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T

K
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K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (= F57), E46 (vs. gap), W64 (≠ C59), N83 (= N78), D84 (= D79), Y92 (≠ P87)
binding coenzyme a: C82 (≠ T77), D84 (= D79), K85 (≠ V80), Y86 (= Y81), P87 (= P82), L114 (= L117), T132 (= T135), K133 (= K136)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
39% identity, 75% coverage: 20:138/159 of query aligns to 7:135/145 of 7s41A

query
sites
7s41A

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

S

N

I

I

G

G

D

S

E

G

T

T

K

R

I

I

G

W

A

Q

F

F

V

C

E

V

I

V

Q

L

G

P

G

S

A

A

T

I

I

I

G

G

A

E

R

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
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F

V

I

-
x
E

-

N

-

D

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V

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K

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I

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G

-

N

-

V

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K

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C

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G

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x
W

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G

G

V

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T

E

I

I

E

E

D

D

E

D

V

V

F

F

V

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
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N

D
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D

V
x
K

Y
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Y

P
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P

R

R

A

S

T

K

T

Q

P
x
Y

D

P

G

K

A

E

L

F

A

-

T

-

A

-

D

-

W

-

T

-

C

-

S

S

P

K

T

T

V

I

K

K

R

K

R

G

A

A

S

S

I

I

G

G

S

A

G

N

A

A

T

T

I

I

L
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L

P

P

N

G

L

I

T

T

I

I

G

G

E

E

N

N

A

A

L

M

V

I

A

G

A

A

G

G

A

A

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T

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K

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V

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (= F57), E46 (vs. gap), W64 (≠ C59), N83 (= N78), D84 (= D79), Y92 (≠ P87)
binding coenzyme a: C82 (≠ T77), D84 (= D79), K85 (≠ V80), Y86 (= Y81), P87 (= P82), L114 (= L117), T132 (= T135), K133 (= K136)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
39% identity, 75% coverage: 20:138/159 of query aligns to 7:135/145 of 7s3wA

query
sites
7s3wA

L

L

V

A

N

D

L

V

Y

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G

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S

N

I

I

G

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A

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F

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V

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V

Q

L

G

P

G

S

A

A

T

I

I

I

G

G

A

E

R

N

C

C

K

N

I

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S

C

F
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F

V

I

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x
E

-

N

-

D

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K

-

I

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G

-

N

-

V

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T

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I

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K

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C

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Q

-

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D

G

G

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I

I

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E

D

D

E

D

V

V

F

F

V

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N

N

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D

V
x
K

Y
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Y

P
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P

R

R

A

S

T

K

T

Q

P
x
Y

D

P

G

K

A

E

L

F

A

-

T

-

A

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D

-

W

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T

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C

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S

S

P

K

T

T

V

I

K

K

R

K

R

G

A

A

S

S

I

I

G

G

S

A

G

N

A

A

T

T

I

I

L
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L

P

P

N

G

L

I

T

T

I

I

G

G

E

E

N

N

A

A

L

M

V

I

A

G

A

A

G

G

A

A

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T

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K

D

D

V

V

binding oxidized coenzyme a: C82 (≠ T77), D84 (= D79), K85 (≠ V80), Y86 (= Y81), P87 (= P82), L114 (= L117), T132 (= T135), K133 (= K136)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F57), E46 (vs. gap), D84 (= D79), Y92 (≠ P87)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
39% identity, 75% coverage: 20:138/159 of query aligns to 7:135/145 of 7s3uA

query
sites
7s3uA

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

S

N

I

I

G

G

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G

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R

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A

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F

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V

Q

L

G

P

G

S

A

A

T

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I

I

G

G

A

E

R

N

C

C

K

N

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I

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C

S

S

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H

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F
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F

V

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x
E

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N

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D

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K

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G

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N

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I

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C

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I

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F

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G

N

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F

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x
C

N
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N

D
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D

V
x
K

Y
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Y

P
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P

R

R

A

S

T

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x
Y

D

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G

K

A

E

L

F

A

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A

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W

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K

T

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I

K

K

R

K

R

G

A

A

S

S

I

I

G

G

S

A

G

N

A

A

T

T

I

I

L
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L

P

P

N

G

L

I

T

T

I

I

G

G

E

E

N

N

A

A

L

M

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A

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A

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G

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V

binding coenzyme a: C82 (≠ T77), N83 (= N78), D84 (= D79), K85 (≠ V80), Y86 (= Y81), P87 (= P82), L114 (= L117), T132 (= T135), K133 (= K136)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F57), E46 (vs. gap), D84 (= D79), Y92 (≠ P87)

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
36% identity, 84% coverage: 25:157/159 of query aligns to 14:157/191 of G3XD01

query
sites
G3XD01

G

G

C

A

S

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R

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G

A

G

G

A

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R

I

I

G

G

A

A

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G

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S

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L

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N

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F

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G

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N

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K

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V

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I

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G

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D

R

R

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C

K

K

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S

N

H

N

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F

S

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Y

E

D

G

N

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T

I

L

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D

E

E

G

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V

F

F

V

C

G

G

H

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G

S

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M

M

V

F

F

T

T

N

N

D

V

V

Y

Y

N

P

P

R

R

A

S

T

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P

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D

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G

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A

-

L

L

A

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A

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A

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D

D

W

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T

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T

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L

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V

K

K

R

K

R

G

A

A

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L

G

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G

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A

C

T

T

I

I

L

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G

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T

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Y

A

A

L

F

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V

A

G

A

A

G

G

A

A

V

V

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K
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K

D

N

V

V

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P

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S

N

Y

A

A

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L

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M

A

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E

K136 (= K136) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
36% identity, 97% coverage: 3:156/159 of query aligns to 12:157/192 of 3mqgC

query
sites
3mqgC

V

V

T

D

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E

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A

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R

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G

K

A

D

H

V

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R

R

I

I

F
x
W

Q

H

P

-

D

-

L

W

V

V

N
x
H

L

I

Y

C

G

G

-

G

C

A

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E

I

I

G

G

D

E

E

G

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C

K
x
S

I

L

G

G

A

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F

N

V

V

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x
F

I

V

Q

G

G

N

G

R

A

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R

I

I

G

G

A

N

R

R

C

V

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K

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I

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x
N

H

N

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F

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x
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E

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N

V

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D

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D

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F
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F

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C

G

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P

G

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M

M

V

F

F

T
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T

N
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N

D
x
V

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x
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Y

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P
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P

R

R

A

A

T

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P

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D

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G

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A

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R

R

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R

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A

A

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G

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x
A

G

N

A

C

T

T

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C

N

G

L

A

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T

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I

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G

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R

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Y

A

A

L
x
F

V

V

A
x
G

A
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A

G

G

A

A

V

V

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x
N

K

K

D

D

V

V

P

P

A

D

N

F

A

A

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A
x
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P

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S

binding acetyl coenzyme *a: Y67 (≠ C59), F77 (= F69), P80 (≠ H72), T85 (= T77), N86 (= N78), V87 (≠ D79), Y88 (≠ V80), P90 (= P82), G112 (= G111), A113 (≠ S112), F128 (≠ L127), G130 (≠ A129), A131 (= A130), N136 (≠ T135), V146 (≠ A145), P149 (= P148), R151 (≠ V150)
binding uridine-5'-monophosphate: H29 (≠ N22), F47 (≠ E39)
binding uridine-5'-diphosphate: W25 (≠ F16), S41 (≠ K33), N62 (≠ S54)

Sites not aligning to the query:

binding acetyl coenzyme *a: 162

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
36% identity, 97% coverage: 3:156/159 of query aligns to 11:156/191 of 3mqhA

query
sites
3mqhA

V

V

T

D

E

E

T

G

V

A

T

R

L

I

G

G

K

A

D

H

V

S

R

R

I

I

F
x
W

Q

H

P

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D

-

L

W

V

V

N
x
H

L

I

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C

G

G

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G

C

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I

I

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G

D

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E

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x
F

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G

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G

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R

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K

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N

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N

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F
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F

V

C

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M

V

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F

T
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T

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N

D
x
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Y

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P
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P

R

R

A

A

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P

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D

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G

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A

A

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A

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R

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W
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T

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R

R

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R

G

A

A

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G

S
x
A

G

N

A

C

T

T

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C

N

G

L

A

T

T

I

I

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N

Y

A

A

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F

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V

A

G

A
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A

G

G

A

A

V

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x
N

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K

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D

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A

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F

A

A

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x
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P

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S

binding coenzyme a: F76 (= F69), T84 (= T77), N85 (= N78), V86 (≠ D79), Y87 (≠ V80), P89 (= P82), G111 (= G111), A112 (≠ S112), A130 (= A130), N135 (≠ T135), V145 (≠ A145), P148 (= P148), R150 (≠ V150)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: W24 (≠ F16), H28 (≠ N22), F46 (≠ E39), Q60 (≠ S53), N61 (≠ S54), Y66 (≠ C59), F76 (= F69), N85 (= N78), V86 (≠ D79), R95 (≠ T93), Y99 (≠ W97)

Sites not aligning to the query:

binding coenzyme a: 161

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
38% identity, 79% coverage: 25:149/159 of query aligns to 104:216/260 of 3fsbA

query
sites
3fsbA

G

N

C

T

S

K

I

I

G

G

D

N

E

N

T

V

K

K

I

I

G
|
G

A

T

F

L

V

S

E

D

I

I

Q

Q

G

H

A

V

T

Y

I

I

G

G

A

N

R

Y

C

V

K

N

I

I

S
x
H

S

S

H

N

S

V

F

F

V

V

C

G

E

E

G

K

V

S

T

I

I

I

E

K

D

D

E

F

V

V

F

W

V

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

V

-

Y

-

P

-

R

-

A

P

T

T

T

P

P

P

D

S

G

N

A

E

L

L

A

L

T

G

A

V

A

T

D

-

W

-

T

-

C

-

S

-

P

-

T

-

V

-

V

I

K

E

R

L

R

F

A

A

S

V

I

I

G
x
A

S
x
A

G

R

A

S

T

V

I

V

L

L

P

P

N

G

L

I

T

H

I

I

G

N

E

E

N

D

A

A

L

L

V

V

A
x
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

A

K

N

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

binding coenzyme a: F150 (≠ G71), A178 (≠ G111), A179 (≠ S112), G196 (≠ A129), A197 (= A130), V212 (≠ A145)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G35), H132 (≠ S53), F150 (≠ G71)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
38% identity, 79% coverage: 25:149/159 of query aligns to 104:216/259 of 3fscA

query
sites
3fscA

G

N

C

T

S

K

I

I

G

G

D

N

E

N

T

V

K
|
K

I

I

G
|
G

A
x
T

F

L

V

S

E

D

I

I

Q

Q

G

H

A

V

T

Y

I

I

G

G

A

N

R

Y

C

V

K

N

I

I

S
x
H

S
|
S

H

N

S

V

F

F

V

V

C

G

E

E

G

K

V

S

T

I

I

I

E

K

D

D

E

F

V

V

F

W

V

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

V

-

Y

-

P

-

R

-

A

P

T

T

T

P

P

P

D

S

G

N

A

E

L

L

A

L

T

G

A

V

A

T

D

-

W

-

T

-

C

-

S

-

P

-

T

-

V

-

V

I

K

E

R

L

R

F

A

A

S

V

I

I

G
x
A

S
x
A

G

R

A

S

T

V

I

V

L

L

P

P

N

G

L

I

T

H

I

I

G

N

E

E

N

D

A

A

L

L

V

V

A
x
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

A

K

N

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

binding coenzyme a: F150 (≠ G71), A178 (≠ G111), A179 (≠ S112), G196 (≠ A129), A197 (= A130), V212 (≠ A145)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (= K33), G114 (= G35), T115 (≠ A36), H132 (≠ S53), S133 (= S54)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
38% identity, 79% coverage: 25:149/159 of query aligns to 104:216/259 of 3fs8A

query
sites
3fs8A

G

N

C

T

S

K

I

I

G

G

D

N

E

N

T

V

K

K

I

I

G

G

A

T

F

L

V

S

E

D

I

I

Q

Q

G

H

A

V

T

Y

I

I

G

G

A

N

R

Y

C

V

K

N

I

I

S

H

S

S

H

N

S

V

F

F

V

V

C

G

E

E

G

K

V

S

T

I

I

I

E

K

D

D

E

F

V

V

F

W

V

L

G
x
F

H
x
P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

V

-

Y

-

P

-

R

-

A

P

T

T

T

P

P

P

D

S

G

N

A

E

L

L

A

L

T

G

A

V

A

T

D

-

W

-

T

-

C

-

S

-

P

-

T

-

V

-

V

I

K

E

R

L

R

F

A

A

S

V

I

I

G
x
A

S
x
A

G

R

A

S

T

V

I

V

L

L

P

P

N

G

L

I

T

H

I

I

G

N

E

E

N

D

A

A

L

L

V

V

A
x
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

A

K

N

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

binding acetyl coenzyme *a: F150 (≠ G71), P151 (≠ H72), A178 (≠ G111), A179 (≠ S112), G196 (≠ A129), A197 (= A130), V212 (≠ A145)

Sites not aligning to the query:

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
30% identity, 78% coverage: 35:158/159 of query aligns to 60:187/200 of 1krrA

query
sites
1krrA

G

N

A

A

F

W

V

V

E

E

I

P

Q

P

G

V

G

Y

A

F

T

S

I

Y

G

G

A

S

R

N

C

I

K

H

I

I

S

G

S

R

H

N

S

F

F

Y

V

A

C
x
N

E

F

G

N

V

L

T

T

I

I

E

V

D

D

E

D

-

Y

-

T

V

V

F

T

V

I

G

G

H

D

G

N

V

V

M

L

F
x
I

T
x
A

N
x
P

D

N

V

V

Y

T

P

L

R

S

A

V

T
|
T

T

G

P
x
H

-

P

-

V

D

H

G

H

A

E

L

L

A

R

T

K

A

N

A

G

D

E

W

M

T

Y

C

S

S

F

P

P

T

I

V

T

V

I

K

G

R

N

A

V

S

W

I

I

G
|
G

S
|
S

G

H

A

V

T

V

I

I

L
x
N

P

P

N

G

L

V

T

T

I

I

G

G

E

D

N

N

A

S

L

V

V

I

A
x
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

N

N

A

V

I

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

V

R

V

V

V

I

G
x
R

E

E

V

I

A

N

D

D

K

R

binding acetyl coenzyme *a: N84 (≠ C59), I103 (≠ F76), A104 (≠ T77), P105 (≠ N78), T112 (= T85), H114 (≠ P87), G140 (= G111), S141 (= S112), N146 (≠ L117), G158 (≠ A129), A159 (= A130), A174 (= A145), P177 (= P148), R182 (≠ G153)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 78% coverage: 35:158/159 of query aligns to 61:188/203 of P07464

query
sites
P07464

G

N

A

A

F

W

V

V

E

E

I

P

Q

P

G

V

G

Y

A

F

T
x
S

I

Y

G

G

A

S

R

N

C

I

K

H

I

I

S

G

S

R

H

N

S

F

F

Y

V

A

C
x
N

E

F

G

N

V

L

T

T

I

I

E

V

D

D

E
x
D

-

Y

-

T

V

V

F

T

V

I

G

G

H

D

G

N

V

V

M

L

F

I

T

A

N

P

D

N

V

V

Y

T

P

L

R

S

A

V

T

T

T

G

P
x
H

-

P

-

V

D

H

G

H

A

E

L

L

A

R

T

K

A

N

A

G

D

E

W

M

T

Y

C

S

S

F

P

P

T

I

V

T

V

I

K

G

R

N

A

V

S

W

I

I

G

G

S
|
S

G

H

A

V

T

V

I

I

L

N

P

P

N

G

L

V

T

T

I

I

G

G

E

D

N

N

A

S

L

V

V

I

A

G

A
|
A

G

G

A

S

V

I

V

V

T
|
T

K
|
K

D

D

V

I

P

P

A

P

N

N

A

V

I

V

V

A

A

A

G

G

V

V

P

P

A

C

V
x
R

V

V

V

I

G
x
R

E

E

V

I

A

N

D

D

K

R

S71 (≠ T45) binding
N85 (≠ C59) binding in other chain; binding in other chain
D93 (≠ E67) binding
H115 (≠ P87) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S112) binding in other chain
A160 (= A130) binding in other chain
TK 165:166 (= TK 135:136) binding
R180 (≠ V150) binding
R183 (≠ G153) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
30% identity, 78% coverage: 35:158/159 of query aligns to 60:187/201 of 1krvA

query
sites
1krvA

G

N

A

A

F

W

V

V

E

E

I

P

Q

P

G

V

G

Y

A

F

T
x
S

I

Y

G

G

A

S

R

N

C

I

K

H

I

I

S

G

S

R

H

N

S

F

F
x
Y

V

A

C
x
N

E

F

G

N

V

L

T

T

I

I

E
x
V

D

D

E
x
D

-

Y

-

T

V

V

F

T

V

I

G

G

H

D

G

N

V

V

M

L

F

I

T
x
A

N

P

D

N

V

V

Y

T

P

L

R
x
S

A

V

T
|
T

T

G

P

H

-

P

-

V

D

H

G

H

A

E

L

L

A

R

T

K

A

N

A

G

D

E

W
x
M

T

Y

C

S

S

F

P

P

T

I

V

T

V

I

K

G

R

N

A

V

S
x
W

I

I

G
|
G

S
|
S

G

H

A

V

T

V

I

I

L
x
N

P

P

N

G

L

V

T

T

I

I

G

G

E

D

N

N

A

S

L

V

V

I

A

G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

N

N

A

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

V
x
R

V

V

V

I

G
x
R

E

E

V

I

A

N

D

D

K

R

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ T45), Y82 (≠ F57), N84 (≠ C59), V90 (≠ E65), D92 (≠ E67), M126 (≠ W97)
binding coenzyme a: A104 (≠ T77), S110 (≠ R83), T112 (= T85), W138 (≠ S109), G140 (= G111), S141 (= S112), N146 (≠ L117), A159 (= A130), P177 (= P148), R179 (≠ V150), R182 (≠ G153)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
30% identity, 78% coverage: 35:158/159 of query aligns to 60:187/201 of 1kruA

query
sites
1kruA

G

N

A

A

F
x
W

V

V

E

E

I

P

Q

P

G

V

G

Y

A

F

T
x
S

I

Y

G

G

A

S

R

N

C

I

K

H

I

I

S

G

S

R

H

N

S

F

F
x
Y

V

A

C
x
N

E

F

G

N

V

L

T

T

I

I

E
x
V

D

D

E
x
D

-

Y

-

T

V

V

F

T

V

I

G

G

H

D

G

N

V

V

M
x
L

F

I

T

A

N

P

D

N

V

V

Y

T

P

L

R

S

A

V

T

T

T

G

P
x
H

-

P

-

V

D

H

G

H

A

E

L

L

A

R

T

K

A

N

A

G

D

E

W
x
M

T

Y

C

S

S

F

P

P

T

I

V

T

V

I

K

G

R

N

A

V

S
x
W

I

I

G
|
G

S
|
S

G

H

A

V

T

V

I

I

L

N

P

P

N

G

L

V

T

T

I

I

G

G

E

D

N

N

A

S

L

V

V

I

A
x
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

N

N

A

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

V

R

V

V

V

I

G
x
R

E

E

V

I

A

N

D

D

K

R

binding coenzyme a: H114 (≠ P87), W138 (≠ S109), G140 (= G111), S141 (= S112), G158 (≠ A129), A159 (= A130), P177 (= P148), R182 (≠ G153)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ F37), S70 (≠ T45), Y82 (≠ F57), Y82 (≠ F57), N84 (≠ C59), V90 (≠ E65), D92 (≠ E67), L102 (≠ M75), H114 (≠ P87), M126 (≠ W97)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
30% identity, 91% coverage: 14:157/159 of query aligns to 26:188/188 of 3igjC

query
sites
3igjC

R

R

I

V

F

E

Q

A

P

K

D

R

L

L

V

T

N

R

L

L

Y

Y

G

N

C

E

S

A

I

V

-

E

-

T

G

G

D

D

E

E

T

R

K

R

I

F

G

T

A

L

F

L

V

N

E

Q

I

L

Q

L

G

G

G

S

A

S

T

A

I

D

G

G

A

K

-

A

-

Q

-

I

-

N

-

P

-

D

-

F

R

R

C

C

-

D

-

Y

-

G

-

Y

-

N

-

I

K

H

I

V

S

G

S

K

H

S

S

F

F
|
F

V

A

C

N

E

F

G

N

V

C

T

V

I

I

E

L

D

D

-

V

-

C

E

E

V

V

F

R

V

I

G

G

H

D

G

H

V

C

M

M

F

F

T
x
A

N

P

D

G

V

V

Y

H

-

I

P
x
Y

R

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A

T

T

T
x
H

P

P

D

L

G

H

A

P

L

V

A

E

-

R

T

N

A

S

A

G

D

K

W

E

T

Y

C

G

S

K

P

P

T

V

V

K

V

I

K

G

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N

A

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S

W

I

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G
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G

S

G

G

G

A

A

T

I

I

I

L
x
N

P

P

N

G

L

V

T

S

I

I

G

G

E

D

N

N

A

A

L

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A

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x
S

G

G

A

A

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T

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D

D

V

V

P

P

A

N

N

N

A

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I

V

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A

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G

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N

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A

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V

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E

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E

binding acetyl coenzyme *a: F84 (= F57), A106 (≠ T77), Y112 (≠ P82), A114 (= A84), H116 (≠ T86), G142 (= G111), N148 (≠ L117), A160 (= A129), S161 (≠ A130), T166 (= T135), N178 (≠ V147)

P71063 UDP-N-acetylbacillosamine N-acetyltransferase; EC 2.3.1.203 from Bacillus subtilis (strain 168) (see paper)
29% identity, 96% coverage: 3:155/159 of query aligns to 78:210/216 of P71063

query
sites
P71063

V

L

T

A

E

E

T

R

V

L

T

G

L

L

G

G

K

K

D

D

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D

-

F

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I

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I

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L

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A

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A

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I

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A

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x
H

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H

-

G

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M

-

F

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T

-

N

-

D

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V

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Y

-

P

P

R

R

A

A

T

T

T

L

P

-

D

S

G

G

A

A

L

V

A

S

T

-

A

-

D

-

W

-

T

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C

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S

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P

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V

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E

R

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A

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H

I

V

G

G

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G

G

A

A

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I

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N

Q

L

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I

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A

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A

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L

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V

A

G

A

A

G

G

A

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A

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A

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H146 (≠ F69) mutation to L: Loss of activity. Affects the oligomeric state.

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
30% identity, 70% coverage: 46:157/159 of query aligns to 68:183/183 of 3nz2C

query
sites
3nz2C

I

F

G

G

A

K

R

T

C

I

K

R

I

I

S

G

S

D

H

H

S

T

F

F

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I

C

N

E

M

G

N

V

V

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V

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M

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L

-

D

-

G

-

A

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P

-

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T

I

I

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G

D

D

E

H

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V

F

L

V

I

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G

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Q

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F

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-

N

-

D

-

V

-

Y
|
Y

P

T

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A

A

S

T
x
H

T

S

P

L

D

D

G

Y

A

R

L

R

A

R

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Q

A

A

A

W

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E

W

T

T

I

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C

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G

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N

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L

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P

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N

G

L

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T

I

I

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A

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L

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A

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x
N

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x
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D

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V

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P

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P

N

D

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L

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x
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G

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P

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A

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L

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x
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L

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D

binding acetyl coenzyme *a: Y107 (= Y81), H111 (≠ T85), G137 (= G111), A155 (= A129), A156 (= A130), N161 (≠ T135), G171 (≠ A145), T173 (≠ V147), P174 (= P148), L178 (≠ V152), R179 (≠ G153)
binding magnesium ion: N157 (≠ G131), T173 (≠ V147)

Query Sequence

>WP_011382553.1 NCBI__GCF_000009985.1:WP_011382553.1
MPVTETVTLGKDVRIFQPDLVNLYGCSIGDETKIGAFVEIQGGATIGARCKISSHSFVCE
GVTIEDEVFVGHGVMFTNDVYPRATTPDGALATAADWTCSPTVVKRRASIGSGATILPNL
TIGENALVAAGAVVTKDVPANAIVAGVPAVVVGEVADKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory