SitesBLAST
Comparing WP_011382583.1 NCBI__GCF_000009985.1:WP_011382583.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 29:285/297 of 6rj3A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 27:283/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G156), I148 (= I158), Y166 (≠ H176), D167 (= D177), P168 (≠ I178), I169 (≠ R179), I170 (≠ D180), H198 (= H208), T199 (≠ L209), L208 (≠ I218), R228 (= R238)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 31:287/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 30:286/303 of 6plgA
7dkmA Phgdh covalently linked to oridonin (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 31:287/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V82), A102 (≠ V110), G148 (= G154), R151 (≠ H157), I152 (= I158), Y170 (≠ H176), D171 (= D177), P172 (≠ I178), I173 (≠ R179), H202 (= H208), T203 (≠ L209), P204 (= P210), T209 (= T215), C230 (≠ A236), A231 (= A237), R232 (= R238), H279 (= H285), G281 (= G287)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
7ewhA Crystal structure of human phgdh in complex with homoharringtonine
37% identity, 80% coverage: 40:293/318 of query aligns to 30:286/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V153), G147 (= G154), L148 (≠ C155), G149 (= G156), R150 (≠ H157), I151 (= I158), G152 (= G159), D170 (= D177), H201 (= H208), T202 (≠ L209), P203 (= P210)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 30:286/302 of 6rihA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 29:285/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N106), A100 (≠ V110), R149 (≠ H157), I150 (= I158), Y168 (≠ H176), D169 (= D177), P170 (≠ I178), I171 (≠ R179), H200 (= H208), T201 (≠ L209), P202 (= P210), T207 (= T215), C228 (≠ A236), A229 (= A237), R230 (= R238), H277 (= H285), G279 (= G287)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
37% identity, 80% coverage: 40:293/318 of query aligns to 35:291/533 of O43175
- T78 (≠ V82) binding
- R135 (vs. gap) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ HI 157:158) binding
- D175 (= D177) binding
- T207 (≠ L209) binding
- CAR 234:236 (≠ AAR 236:238) binding
- D260 (= D262) binding
- V261 (= V263) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HVGG 285:288) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 80% coverage: 40:293/318 of query aligns to 30:286/301 of 6rj5A
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
38% identity, 71% coverage: 67:293/318 of query aligns to 49:277/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ H157), Y160 (≠ H176), D161 (= D177), P162 (≠ I178), I164 (≠ D180), L179 (= L195), T193 (≠ L209), P194 (= P210), S198 (= S214), L202 (≠ I218)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
40% identity, 73% coverage: 63:293/318 of query aligns to 54:286/525 of 3ddnB
Sites not aligning to the query:
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
40% identity, 73% coverage: 63:293/318 of query aligns to 53:285/526 of 3dc2A
Sites not aligning to the query:
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 77% coverage: 54:298/318 of query aligns to 47:301/334 of 5aovA
- active site: L100 (≠ N106), R241 (= R238), D265 (= D262), E270 (= E267), H288 (= H285)
- binding glyoxylic acid: M52 (≠ A59), L53 (= L60), L53 (= L60), Y74 (= Y80), A75 (≠ G81), V76 (= V82), G77 (= G83), R241 (= R238), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V82), T104 (≠ V110), F158 (≠ C155), G159 (= G156), R160 (≠ H157), I161 (= I158), S180 (≠ D177), R181 (≠ I178), A211 (≠ H208), V212 (≠ L209), P213 (= P210), T218 (= T215), I239 (≠ A236), A240 (= A237), R241 (= R238), H288 (= H285), G290 (= G287)
7cvpA The crystal structure of human phgdh from biortus.
37% identity, 65% coverage: 88:293/318 of query aligns to 33:240/254 of 7cvpA
- binding nicotinamide-adenine-dinucleotide: G101 (= G154), G103 (= G156), R104 (≠ H157), I105 (= I158), Y123 (≠ H176), D124 (= D177), P125 (≠ I178), I126 (≠ R179), H155 (= H208), T156 (≠ L209), P157 (= P210), T162 (= T215), C183 (≠ A236), A184 (= A237), R185 (= R238), H232 (= H285), G234 (= G287)
7va1A Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with gdd-04-35
38% identity, 59% coverage: 106:293/318 of query aligns to 1:190/193 of 7va1A
- binding 4-[(3-ethanoylphenyl)sulfamoyl]-~{N}-[4-(3-fluorophenyl)-1,3-thiazol-2-yl]benzamide: L50 (≠ V153), G53 (= G156), R57 (≠ K160), Y73 (≠ H176), D74 (= D177), P75 (≠ I178), I76 (≠ R179), I77 (≠ D180), T106 (≠ L209), P107 (= P210), L115 (≠ I218)
5ofwA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 3-chloro-4-fluorobenzamide (see paper)
38% identity, 59% coverage: 106:293/318 of query aligns to 3:192/195 of 5ofwA
- active site: N3 (= N106), R137 (= R238), D161 (= D262), E166 (= E267), H184 (= H285)
- binding 3-chloranyl-4-fluoranyl-benzamide: G53 (= G154), Y75 (≠ H176), P77 (≠ I178), T108 (≠ L209), S113 (= S214), T114 (= T215), L117 (≠ I218)
5ofvA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 5-fluoro-2-methylbenzoic acid (see paper)
38% identity, 59% coverage: 106:293/318 of query aligns to 3:192/195 of 5ofvA