SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011382704.1 NCBI__GCF_000009985.1:WP_011382704.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

3wkuA Crystal structure of the anaerobic desb-gallate complex (see paper)
26% identity, 91% coverage: 9:114/117 of query aligns to 298:403/407 of 3wkuA

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3wkuA

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binding 3,4,5-trihydroxybenzoic acid: F368 (≠ Y79), E371 (≠ A82), Y385 (≠ G96)

Sites not aligning to the query:

active site: 11, 53, 186, 233
binding fe (iii) ion: 11, 51, 53, 233
binding 3,4,5-trihydroxybenzoic acid: 406

3wr9A Crystal structure of the anaerobic desb-gallate complex (see paper)
26% identity, 91% coverage: 9:114/117 of query aligns to 303:408/416 of 3wr9A

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3wr9A

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binding 3,4,5-trihydroxybenzoic acid: E376 (≠ A82), Y390 (≠ G96)

Sites not aligning to the query:

active site: 11, 58, 191, 238
binding fe (ii) ion: 11, 19, 58, 135, 238
binding 3,4,5-trihydroxybenzoic acid: 12, 13, 58, 123, 191, 266, 411

Query Sequence

>WP_011382704.1 NCBI__GCF_000009985.1:WP_011382704.1
MAPKTKRVIPGTTLFDGDMARKGYALNKMCFSFNSEENRQEFLKDPGAYCDKFGLNEQQK
KAVISLQVLDMLAAGGNAYFIAKLGGIYKLDMQDVGAQQTGVTKEEFQAKLVAAGRE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory