SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011382976.1 NCBI__GCF_000009985.1:WP_011382976.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8U1K9 NAD(P)H:rubredoxin oxidoreductase; NROR; Rubredoxin--NAD(P)(+) reductase; EC 1.18.1.4 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
31% identity, 45% coverage: 8:377/819 of query aligns to 2:344/359 of Q8U1K9

query
sites
Q8U1K9

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G11 (≠ A17) binding
DK 29:30 (≠ GA 39:40) binding
KP 38:39 (≠ RI 48:49) binding
A75 (≠ V85) binding
A103 (≠ S114) binding
R120 (= R133) binding
D260 (≠ E282) binding

Q9AL95 NADH-rubredoxin oxidoreductase; NROR; NADH:rubredoxin oxidoreductase; EC 1.18.1.1 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) (see paper)
29% identity, 46% coverage: 4:381/819 of query aligns to 1:354/379 of Q9AL95

query
sites
Q9AL95

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C26 (≠ A29) modified: Disulfide link with 286
NSE 33:35 (≠ GAE 39:41) binding
R42 (= R48) binding
A79 (≠ V85) binding
Y125 (≠ R133) binding
C137 (≠ A145) modified: Disulfide link with 216
C216 (≠ L239) modified: Disulfide link with 137
D259 (≠ E282) binding
C286 (≠ A309) modified: Disulfide link with 26

3kljA Crystal structure of nadh:rubredoxin oxidoreductase from clostridium acetobutylicum (see paper)
29% identity, 46% coverage: 8:381/819 of query aligns to 4:353/378 of 3kljA

query
sites
3kljA

K

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active site: P12 (≠ M16), R41 (= R48), P42 (≠ I49), N274 (≠ W298)
binding flavin-adenine dinucleotide: L8 (≠ I12), G9 (= G13), G11 (= G15), P12 (≠ M16), A13 (= A17), N32 (≠ G39), S33 (≠ A40), E34 (= E41), R41 (= R48), P42 (≠ I49), F77 (≠ P84), A78 (≠ V85), S105 (≠ T112), G106 (= G113), L123 (≠ F132), Y124 (≠ R133), L150 (= L159), G257 (= G281), D258 (≠ E282), G268 (= G292), L269 (= L293), I270 (≠ V294), A273 (≠ I297), K301 (= K326)

1xhcA Nadh oxidase /nitrite reductase from pyrococcus furiosus pfu-1140779- 001
31% identity, 45% coverage: 7:377/819 of query aligns to 1:339/346 of 1xhcA

query
sites
1xhcA

Q

S

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V

K

A

L

I

I

D

D

R

R

A

G

A

R

K

K

T

V

V

V

T

I

S

T

A

E

N

K

G

G

V

E

V

V

V

-

P

P

Y

Y

D

D

K

T

L

L

L

V

L

L

S
x
A

T
|
T

G
|
G

S

A

K

R

P

A

L

R

M

E

P

P

P

Q

L

I

P

K

G

G

L

K

D

E

L

Y

P

-

G

-

V

L

A

T

F
x
L

R
|
R

D

T

I

I

A

F

D

D

V

A

E

D

K

R

M

I

L

K

D

E

A

S

A

I

E

E

A

N

K

S

Q

G

R

E

A

A

V

I

I

I

G

G

L
x
F

L
x
I

G

G

L

L

E
|
E

A

L

A

A

W

G

G

N

L

L

K

A

R

E

R

A

G

G

M

Y

P

H

V

V

A

K

L

L

V

I

H

H

L

R

M

G

P

A

T

M

L

F

M

L

E

-

R

-

Q

G

L

L

D

D

V

E

E

E

A

L

G

S

G

N

L

M

L

I

Q

K

K

D

D

M

L

L

T

E

E

E

R

T

G

G

L

V

H

K

F

F

T

L

S

N

G

S

Q

E

T

L

E

L

E

E

I

-

M

-

G

-

A

-

E

-

T

-

G

A

R

N

A

E

Q

E

G

G

V

V

K

-

L

L

T

T

D

N

G

S

R

G

E

F

I

I

P

E

A

G

D

K

L

V

V

K

V

I

V

C

A

A

I

I

G

G

I

I

R

V

P

P

N

N

V

V

D

D

L

L

A

A

K

R

A

R

A

S

G

G

L

I

D

H

I

T

N

G

R

R

G

G

I

I

E

L

V

I

G

D

D

D

D

N

M

F

A

R

T

T

S

S

D

A

P

K

A

D

I

V

Y

Y

S

A

V

I

G
|
G

E
x
D

C

C

V

A

E

E

H

Y

R

S

G

G

Q

I

I

I

F

A

G
|
G

L
x
T

V
x
A

A

K

P

A

I

A

W
x
M

E

E

Q

Q

A

A

K

R

V

V

C

L

A

A

S

D

R

I

L

L

A

K

G

G

R

-

D

E

D

P

A

R

H

R

Y

Y

E

N

T

F

P

K

P

F

L

R

S

S

T

T

R

V

L

F

K

K

I

F

T

G

G

K

I

L

D

Q

V

I

F

A

S

I

A

I

G

G

Q

N

L

T

A

K

A

G

Q

E

D

G

E

K

A

W

D

I

E

E

E

-

L

-

V

-

Y

-

R

-

D

D

S

N

A

T

R

K

G

V

I

F

Y

Y

K

-

L

-

V

-

I

-

R

-

A

-

D

-

K

-

V

-

V

I

G

G

A

A

V

V

M

V

Y

F

G

N

D

D

V

I

active site: P10 (≠ M16), K38 (≠ R48), P39 (≠ I49), F145 (≠ L158), E149 (= E162), M276 (≠ W298)
binding flavin-adenine dinucleotide: V6 (≠ I12), G7 (= G13), G9 (= G15), P10 (≠ M16), G11 (≠ A17), D29 (≠ G39), K30 (≠ A40), K38 (≠ R48), P39 (≠ I49), E74 (≠ P84), A75 (≠ V85), A100 (≠ S111), T101 (= T112), G102 (= G113), L119 (≠ F132), R120 (= R133), F145 (≠ L158), I146 (≠ L159), E149 (= E162), G259 (= G281), D260 (≠ E282), G270 (= G292), T271 (≠ L293), A272 (≠ V294)

6pfzA Structure of a NAD-dependent persulfide reductase from a. Fulgidus (see paper)
37% identity, 27% coverage: 88:310/819 of query aligns to 89:321/541 of 6pfzA

query
sites
6pfzA

I

I

D

D

R

R

A

S

A

R

K

K

T

T

V

V

T

K

S

I

A

V

-

R

N

N

G

G

V

S

V

E

-

D

-

E

V

L

P

N

Y

Y

D

D

K

Y

L

L

L

V

L

I

S
x
A

T
|
T

G
|
G

S

A

K

R

P

P

L

A

M

K

P

P

L

I

P

E

G

G

L

I

D

E

L

A

P

E

G

G

V

V

A

T

F
x
L

R

T

D

S

I

A

A

E

D

E

V

A

E

E

K

K

M

I

L

I

D

E

A

M

-

W

A

E

E

E

A

G

K

A

Q

E

R

K

A

A

V

V

I

I

G

G

G

A

G

G

L

F

L

I

G

G

L

L

E

E

A

S

A

A

W

E

G

A

L

L

K

K

R

N

R

L

G

D

M

M

P

E

V

V

A

T

L

V

V

I

H

E

L

M

M

M

P

D

T

R

L

V

M

A

E

P

R

A

Q

M

L

L

D

D

V

R

E

E

A

M

G

A

G

V

L

L

L

V

Q

E

K

N

D

H

L

L

T

R

E

E

R

K

G

G

L

V

H

N

F

V

T

T

S

S

G

T

Q

R

T

V

E

E

E

K

I

I

M

V

G

-

A

S

E

Q

T

D

G

D

R

K

A

V

Q

R

G

A

V

V

K

-

L

I

T

A

D

N

G

G

R

K

E

E

I

Y

P

P

A

A

D

D

L

V

V

V

A

A

I

T

G

G

I

I

R

K

P

P

N

N

V

S

D

E

L

L

A

A

K

E

A

K

A

A

G

G

L

L

D

K

I

I

N

G

R

E

-

T

-

G

G

A

I

I

E

W

V

V

G

D

D

E

D

Y

M

M

A

R

T

T

S

S

D

D

P

E

A

S

I

I

Y

Y

S

A

V

G

G
|
G

E
x
D

C

C

V

V

E

E

H

T

R

T

G

C

Q

L

I

V

F

T

G

G

-

K

L

I

V

I

A

A

P
|
P

I
x
F

W
x
G

E
x
D

-

V

-

A

-

N

-

K

Q

Q

A

G

K
x
R

V

V

C

I

A

G

S

E

R

N

L

I

A

T

G

G

binding calcium ion: D306 (≠ E299)
binding coenzyme a: R313 (≠ K302)
binding flavin-adenine dinucleotide: A115 (≠ S111), T116 (= T112), G117 (= G113), L136 (≠ F132), G286 (= G281), D287 (≠ E282), P303 (= P296), F304 (≠ I297), G305 (≠ W298)

Sites not aligning to the query:

active site: 37, 42, 455, 456
binding calcium ion: 332, 427, 428
binding coenzyme a: 14, 18, 21, 38, 40, 42, 76, 430, 447, 512, 513, 516
binding flavin-adenine dinucleotide: 7, 11, 32, 33, 41, 42, 86

3fg2P Crystal structure of ferredoxin reductase for the cyp199a2 system from rhodopseudomonas palustris (see paper)
33% identity, 38% coverage: 9:320/819 of query aligns to 4:318/404 of 3fg2P

query
sites
3fg2P

L

V

V

L

V

I

I

A

G
|
G

N

A

G
|
G

M
x
H

A
|
A

G

G

M

F

R

Q

T

V

V

A

E

V

E

S

L

L

-

R

L

Q

A

A

I

K

A

Y

P

P

A

G

R

R

Y

-

D

-

I

I

T

A

V

L

F

I

G

N

A
x
D

E
|
E

P

K

H

H

P

L

N

P

Y

Y

N

Q

R
|
R

I
x
P

M

P

L

L

S
|
S

-
x
K

S

A

V

Y

L

L

A

K

G

S

E

G

K

G

Q

D

V

P

D

N

D

S

I

L

V

M

I

F

N

R

P

P

R

E

A

K

W

F

Y

F

A

Q

E

D

N

Q

G

A

I

I

T

E

L

L

H

-

T

I

G

S

D

D

P
x
R

V
x
M

V

V

A

S

I

I

D

D

R

R

A

E

A

G

K

R

T

K

V

L

T

L

S

L

A

A

N

S

G

G

V

T

V

A

V

I

P

E

Y

Y

D

G

K

H

L

L

L

V

L

L

S

A

T
|
T

G

G

S

A

K

R

P

N

L

R

M

M

P

L

P

D

L

V

P

P

G

N

L

A

D

S

L

L

P

P

G

D

V

V

V

L

A

Y

F

L

R
|
R

D

T

I

L

A

D

D

E

V

S

E

E

K

V

M

L

L

R

D

Q

A

R

A

M

E

P

A

D

K

K

Q

K

R

H

A

V

V

V

I

I

G

G

G

A

G

G

L

F

L
x
I

G

G

L

L

E

E

A

F

A

A

W

A

G

T

L

A

K

R

R

A

R

K

G

G

M

L

P

E

V

V

A

D

L

V

V

V

H

E

L

L

M

A

P

P

T

R

L

V

M

M

E

A

R

R

Q

V

L

V

D

T

V

P

E

E

A

I

G

S

L

Y

L

F

Q

H

K

D

D

R

L

H

T

S

E

G

R

A

G

G

L

I

H

R

F

M

F

H

T

Y

S

G

G

V

Q

R

T

A

E

T

E

E

I

I

M

-

G

A

A

A

E

E

T

G

G

D

R

R

A

V

Q

T

G

G

V

V

K

V

L

L

T

S

D

D

G

G

R

N

E

T

I

L

P

P

A

C

D

D

L

L

V

V

A

G

I

V

G

G

I

V

R

I

P

P

N

N

V

V

D

E

L

I

A

A

K

A

A

A

G

G

L

L

D

P

I

T

N

A

R

A

G

G

I

I

E

I

V

V

G

D

D

Q

M

L

A

L

T

T

S

S

D

D

P

P

A

H

I

I

Y

S

S

A

V

I

G

G

E
x
D

C

C

V

A

E

L

H

F

R

E

G

S

Q

V

I

R

F

F

G

G

-

E

-

T

-

M

-

R

-

V

-

E

L
x
S

V
|
V

A

Q

P

N

I

A

W

T

E

D

Q

Q

A

A

K

R

V

C

C

V

A

A

S

A

R

R

L

L

A

T

G

G

R

-

D

D

D

A

A

K

H

P

Y

Y

E

D

T

G

P

Y

P

P

binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), H11 (≠ M16), A12 (= A17), D34 (≠ A40), E35 (= E41), R42 (= R48), P43 (≠ I49), S46 (= S52), K47 (vs. gap), R78 (≠ P84), M79 (≠ V85), T106 (= T112), R127 (= R133), I153 (≠ L159), D275 (≠ E282), S292 (≠ L293), V293 (= V294)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 321

6rvhA Nadh-dependent coenzyme a disulfide reductase soaked with menadione (see paper)
31% identity, 36% coverage: 45:339/819 of query aligns to 40:335/443 of 6rvhA

query
sites
6rvhA

N
x
S

Y
|
Y

N

G

R
x
A

I
x
C

M

G

L

L

S

P

S

Y

V

V

L

L

A

S

G

G

E
|
E

-

I

K

P

Q

R

V

L

D
x
E

D

R

I

L

V

V

I

A

N
x
R

P

T

R

P

A

E

W

E

Y

F

A

R

E

K

N

Q

G

G

I

V

T

L

L

V

H

H

T

T

G

R

D

H

P

E

V
|
V

V

V

A

D

I

V

D

D

R

Y

A

E

A

L

K

R

T

T

V

L

T

T

-

V

-

H

-

D

S

H

A

A

N

E

G

G

V

R

V

T

V

F

P

Q

-

D

-

R

Y

F

D

D

K

H

L

L

L

V

L

L

S

A

T
|
T

G
|
G

S

A

K

R

P

P

L

S

M

L

P

P

L

I

P

P

G

G

L

T

D

E

L

Q

P

E

G

G

V

V

V

Y

A

T

F

L

R
|
R

D

T

I

M

A

E

D

D

V

G

E

E

K

R

M

L

L

L

D

K

A

A

A

L

E

P

A

Q

K

A

Q

R

R

R

A

A

V

A

V

I

I

L

G

G

G

A

G

G

L
x
Y

L

I

G

G

L

L

E
|
E

A

A

W

E

G

A

L

F

K

R

R

K

R

R

G

G

M

L

P

Q

V

V

A

T

L

L

V

L

H

E

L

A

M

K

P

D

T

R

L

P

M

L

E

P

R

-

Q

H

L

W

D

D

V

P

E

E

A

V

G

G

G

A

L

L

Q

K

K

E

D

E

L

L

T

E

E

R

R

H

G

G

L

V

H

E

F

V

F

W

T

T

S

G

G

V

Q

K

T

V

E

E

E

A

I

F

M

R

G

G

A

-

E

-

T

M

G

G

R

R

A

V

Q

E

G

A

V

V

K

E

L

T

T

S

D

E

G

G

R

-

E

V

I

V

P

P

A

A

D

D

L

L

V

V

V

L

A

A

I

T

G

G

I

I

R

R

P

P

N

N

V

T

D

E

L

L

A

A

K

Q

A

A

A

M

G

G

L

V

D

A

I

L

N

G

R

P

-

T

-

G

G

A

I

I

E

A

V

T

G

D

D

E

D

R

M

M

A

R

T

T

S

N

D

L

P

E

A

G

I

V

Y

Y

S

A

V

A

G
|
G

E
x
D

C

V

V

A

E

E

H

S

R

F

G

H

Q

R

I

V

F

-

G

-

L

L

V

K

A

R

P

P

I

Y

W

W

-

L

-
x
P

-
x
L

-
x
G

-

D

-

V

-

A

-
x
N

E

K

Q

H

A

G

K
x
R

V

T

C

A

A

G

S

S

R

V

L

I

A

A

G

G

R

R

D

E

D

-

A

-

H

-

Y

-

E

-

T

-

P

-

L

-

S

A

T

R

R

F

L

L

K

G

I

V

T

V

G

G

I

T

D

A

V

I

F

F

S

K

A

A

G

F

Q

D

L

L

A

A

active site: C44 (≠ I49), E53 (= E58), E58 (≠ D62), Y159 (≠ L158), E163 (= E162)
binding coenzyme a: S40 (≠ N45), Y41 (= Y46), C44 (≠ I49), R63 (≠ N67), N303 (vs. gap), R307 (≠ K302)
binding flavin-adenine dinucleotide: A43 (≠ R48), C44 (≠ I49), V81 (= V85), T113 (= T112), G114 (= G113), R134 (= R133), Y159 (≠ L158), G280 (= G281), D281 (≠ E282), P297 (vs. gap), L298 (vs. gap), G299 (vs. gap)

Sites not aligning to the query:

active site: 39, 425, 430
binding coenzyme a: 20, 23, 359, 431, 437, 441, 442
binding flavin-adenine dinucleotide: 8, 11, 12, 13, 34, 35, 424, 425
binding menadione: 364, 365, 430, 431

6rvbA Nadh-dependent coenzyme a disulfide reductase soaked with nadh (see paper)
31% identity, 36% coverage: 45:339/819 of query aligns to 40:335/443 of 6rvbA

query
sites
6rvbA

N
x
S

Y
|
Y

N

G

R
x
A

I
x
C

M

G

L

L

S

P

S

Y

V

V

L

L

A

S

G

G

E
|
E

-

I

K

P

Q

R

V

L

D
x
E

D

R

I

L

V

V

I

A

N
x
R

P

T

R

P

A

E

W

E

Y

F

A

R

E

K

N

Q

G

G

I

V

T

L

L

V

H

H

T

T

G

R

D

H

P
x
E

V
|
V

V

V

A

D

I

V

D

D

R

Y

A

E

A

L

K

R

T

T

V

L

T

T

-

V

-

H

-

D

S

H

A

A

N

E

G

G

V

R

V

T

V

F

P

Q

-

D

-

R

Y

F

D

D

K

H

L

L

L

V

L

L

S

A

T
|
T

G
|
G

S

A

K

R

P

P

L

S

M

L

P

P

L

I

P

P

G

G

L

T

D

E

L

Q

P

E

G

G

V

V

V

Y

A

T

F
x
L

R
|
R

D

T

I

M

A

E

D

D

V

G

E

E

K

R

M

L

L

L

D

K

A

A

A

L

E

P

A

Q

K

A

Q

R

R

R

A

A

V

A

V

I

I

L

G
|
G

G

A

G
|
G

L
x
Y

L
x
I

G

G

L

L

E
|
E

A

A

W

E

G

A

L

F

K

R

R

K

R

R

G

G

M

L

P

Q

V

V

A

T

L

L

V

L

H
x
E

L
x
A

M

K

P

D

T

R

L

P

M

L

E

P

R

-

Q

H

L

W

D

D

V

P

E

E

A

V

G

G

G

A

L

L

Q

K

K

E

D

E

L

L

T

E

E

R

R

H

G

G

L

V

H

E

F

V

F

W

T

T

S

G

G

V

Q

K

T

V

E

E

E

A

I

F

M

R

G

G

A

-

E

-

T

M

G

G

R

R

A

V

Q

E

G

A

V

V

K

E

L

T

T

S

D

E

G

G

R

-

E

V

I

V

P

P

A

A

D

D

L

L

V

V

V

L

A
|
A

I
x
T

G
|
G

I

I

R

R

P

P

N

N

V

T

D

E

L

L

A

A

K

Q

A

A

A

M

G

G

L

V

D

A

I

L

N

G

R

P

-

T

-

G

G

A

I

I

E

A

V

T

G

D

D

E

D

R

M

M

A

R

T

T

S

N

D

L

P

E

A

G

I

V

Y

Y

S

A

V

A

G
|
G

E
x
D

C

V

V

A

E

E

H

S

R

F

G

H

Q

R

I

V

F

-

G

-

L

L

V

K

A

R

P

P

I

Y

W

W

-

L

-
x
P

-
x
L

-
x
G

-

D

-

V

-

A

-
x
N

E

K

Q

H

A

G

K
x
R

V

T

C

A

A

G

S

S

R

V

L

I

A

A

G

G

R

R

D

E

D

-

A

-

H

-

Y

-

E

-

T

-

P

-

L

-

S

A

T

R

R

F

L

L

K

G

I

V

T

V

G

G

I

T

D

A

V
x
I

F
|
F

S

K

A

A

G

F

Q

D

L

L

A

A

active site: C44 (≠ I49), E53 (= E58), E58 (≠ D62), Y159 (≠ L158), E163 (= E162)
binding coenzyme a: S40 (≠ N45), Y41 (= Y46), C44 (≠ I49), R63 (≠ N67), N303 (vs. gap), R307 (≠ K302)
binding flavin-adenine dinucleotide: A43 (≠ R48), C44 (≠ I49), E80 (≠ P84), V81 (= V85), T113 (= T112), G114 (= G113), L133 (≠ F132), R134 (= R133), I160 (≠ L159), G280 (= G281), D281 (≠ E282), L298 (vs. gap), G299 (vs. gap)
binding nicotinamide-adenine-dinucleotide: G156 (= G155), G158 (= G157), Y159 (≠ L158), I160 (≠ L159), E179 (≠ H178), A180 (≠ L179), A240 (= A243), T241 (≠ I244), G242 (= G245), P297 (vs. gap), I328 (≠ V332), F329 (= F333)

Sites not aligning to the query:

active site: 39, 425, 430
binding coenzyme a: 15, 20, 23, 359, 437, 441, 442
binding flavin-adenine dinucleotide: 8, 11, 12, 13, 33, 34, 35, 36, 424, 425

6ruzA Nadh-dependent coenzyme a disulfide reductase (see paper)
31% identity, 36% coverage: 45:339/819 of query aligns to 40:335/443 of 6ruzA

query
sites
6ruzA

N
x
S

Y
|
Y

N

G

R
x
A

I
x
C

M

G

L

L

S

P

S

Y

V

V

L

L

A

S

G

G

E
|
E

-

I

K

P

Q

R

V

L

D
x
E

D

R

I

L

V

V

I

A

N
x
R

P

T

R

P

A

E

W

E

Y

F

A

R

E

K

N

Q

G

G

I

V

T

L

L

V

H

H

T

T

G

R

D

H

P
x
E

V
|
V

V

V

A

D

I

V

D

D

R

Y

A

E

A

L

K

R

T

T

V

L

T

T

-

V

-

H

-

D

S

H

A

A

N

E

G

G

V

R

V

T

V

F

P

Q

-

D

-

R

Y

F

D

D

K

H

L

L

L

V

L

L

S

A

T
|
T

G
|
G

S
x
A

K

R

P

P

L

S

M

L

P

P

L

I

P

P

G

G

L

T

D

E

L

Q

P

E

G

G

V

V

V

Y

A

T

F
x
L

R
|
R

D

T

I

M

A

E

D

D

V

G

E

E

K

R

M

L

L

L

D

K

A

A

A

L

E

P

A

Q

K

A

Q

R

R

R

A

A

V

A

V

I

I

L

G

G

G

A

G

G

L
x
Y

L

I

G

G

L

L

E
|
E

A

A

W

E

G

A

L

F

K

R

R

K

R

R

G

G

M

L

P

Q

V

V

A

T

L

L

V

L

H

E

L

A

M

K

P

D

T

R

L

P

M

L

E

P

R

-

Q

H

L

W

D

D

V

P

E

E

A

V

G

G

G

A

L

L

Q

K

K

E

D

E

L

L

T

E

E

R

R

H

G

G

L

V

H

E

F

V

F

W

T

T

S

G

G

V

Q

K

T

V

E

E

E

A

I

F

M

R

G

G

A

-

E

-

T

M

G

G

R

R

A

V

Q

E

G

A

V

V

K

E

L

T

T

S

D

E

G

G

R

-

E

V

I

V

P

P

A

A

D

D

L

L

V

V

V

L

A

A

I

T

G

G

I

I

R

R

P

P

N

N

V

T

D

E

L

L

A

A

K

Q

A

A

A

M

G

G

L

V

D

A

I

L

N

G

R

P

-

T

-

G

G

A

I

I

E

A

V

T

G

D

D

E

D

R

M

M

A

R

T

T

S

N

D

L

P

E

A

G

I

V

Y

Y

S

A

V

A

G
|
G

E
x
D

C

V

V

A

E

E

H

S

R

F

G

H

Q

R

I

V

F

-

G

-

L

L

V

K

A

R

P

P

I

Y

W

W

-

L

-
x
P

-
x
L

-
x
G

-

D

-

V

-

A

-
x
N

E

K

Q

H

A

G

K
x
R

V

T

C

A

A

G

S

S

R

V

L

I

A

A

G

G

R

R

D

E

D

-

A

-

H

-

Y

-

E

-

T

-

P

-

L

-

S

A

T

R

R

F

L

L

K

G

I

V

T

V

G

G

I

T

D

A

V

I

F

F

S

K

A

A

G

F

Q

D

L

L

A

A

active site: C44 (≠ I49), E53 (= E58), E58 (≠ D62), Y159 (≠ L158), E163 (= E162)
binding coenzyme a: S40 (≠ N45), Y41 (= Y46), C44 (≠ I49), R63 (≠ N67), N303 (vs. gap), R307 (≠ K302)
binding flavin-adenine dinucleotide: A43 (≠ R48), C44 (≠ I49), E80 (≠ P84), V81 (= V85), T113 (= T112), G114 (= G113), A115 (≠ S114), L133 (≠ F132), R134 (= R133), Y159 (≠ L158), G280 (= G281), D281 (≠ E282), P297 (vs. gap), L298 (vs. gap), G299 (vs. gap)

Sites not aligning to the query:

active site: 39, 425, 430
binding coenzyme a: 15, 19, 20, 23, 359, 441, 442
binding flavin-adenine dinucleotide: 8, 11, 12, 13, 33, 34, 35, 424, 425

3nt6A Structure of the shewanella loihica pv-4 nadh-dependent persulfide reductase c43s/c531s double mutant (see paper)
31% identity, 31% coverage: 64:318/819 of query aligns to 60:345/565 of 3nt6A

query
sites
3nt6A

I

V

V

L

I

Q

N

T

P

P

R

E

A

S

W

F

Y

K

A

A

E

R

N

F

G

N

I

V

T

E

L

V

H

R

T

V

G

K

D

H

P
x
E

V
|
V

V

V

A

A

I

I

D

D

R

R

A

A

K

K

T

L

V

V

T

T

-

V

-

R

-

L

S

D

A

G

N

S

G

E

V

Y

V

Q

V

E

P

S

Y

Y

D

D

K

T

L

L

S
|
S

T
x
P

G
|
G

S

A

K

A

P

P

L

I

M

V

P

P

L

I

P

P

G

G

L

V

D

D

L

N

P

P

G

L

V

T

V

H

A

S

F
x
L

R
|
R

D

N

I

I

A

P

D

D

V

M

E

D

K

R

M

I

L

L

D

Q

A

T

A

I

E

Q

A

M

K

N

-

N

-

V

Q

E

R

H

A

A

V

T

V

V

I

V

G

G

L
x
F

L

I

G

G

L

L

E

E

A

M

W

E

G

S

L

L

K

H

R

H

R

L

G

G

M

I

P

K

V

T

A

T

L

L

V

L

H

E

L

L

M

A

P

D

T

Q

L

V

M

M

E

-

R

T

Q

P

L

V

D

D

V

R

E

E

A

M

G

A

G

G

L

F

L

A

Q

H

K

Q

D

A

L

I

T

R

E

D

R

Q

G

G

L

V

H

D

F

L

F

R

T

L

S

G

G

T

Q

A

T

L

E

S

E

E

I

V

-

S

-

Y

-

Q

-

V

-

Q

-

T

-

H

-

V

-

A

-

S

-

D

-

A

M

A

G

G

A

E

E

D

T

T

G

A

R

H

A

Q

Q

H

-

I

-

K

-

G

-

H

-

L

G

S

V

L

K

T

L

L

T

S

D

N

G

G

R

E

E

L

I

L

P

E

A

T

D

D

L

L

V

L

V

I

V

M

A

A

I

I

G

G

I

V

R

R

P

P

N

E

V

T

D

Q

L

L

A

A

K

R

A

D

A

A

G

G

L

L

D

A

I

I

N

G

R

E

-

L

-

G

G

G

I

I

E

K

V

V

G

N

D

A

D

M

M

M

A

Q

T

T

S

S

D

D

P

P

A

A

I

I

Y

Y

S

A

V

V

G
|
G

E
x
D

C

A

V

V

E

E

H

E

R

Q

G

D

Q

F

I

V

F

T

G

G

-

Q

-

A

-

C

-

L

-

V

-
x
P

L
|
L

V
x
A

A

G

P

P

I

A

W
x
N

E

R

Q

Q

A

G

K
x
R

V

M

C

A

A

A

S

D

R

N

L

M

A

F

G

G

R

R

D

E

A

R

H

Y

Y

Q

E

G

T

T

active site: N325 (≠ W298)
binding coenzyme a: A321 (≠ V294), N325 (≠ W298), R329 (≠ K302)
binding flavin-adenine dinucleotide: E80 (≠ P84), V81 (= V85), S112 (= S111), P113 (≠ T112), G114 (= G113), L133 (≠ F132), R134 (= R133), F161 (≠ L158), G302 (= G281), D303 (≠ E282), P319 (vs. gap), L320 (= L293), A321 (≠ V294)

Sites not aligning to the query:

active site: 11, 38, 42, 43, 471, 472
binding coenzyme a: 11, 18, 19, 22, 39, 42, 43, 459, 462, 463, 466, 534, 535
binding flavin-adenine dinucleotide: 7, 8, 10, 11, 12, 33, 34, 42, 43

3lxdA Crystal structure of ferredoxin reductase arr from novosphingobium aromaticivorans (see paper)
35% identity, 31% coverage: 35:286/819 of query aligns to 31:283/409 of 3lxdA

query
sites
3lxdA

I

V

T

L

V

V

F

I

G

G

A
x
R

E
|
E

P

P

H

E

P

I

N

P

Y

Y

N

E

R
|
R

I
x
P

M

P

L

L

S
|
S

S
x
K

-

E

V

Y

L

L

A

A

G

R

E

E

K

K

Q

T

V

F

D

E

D

R

I

I

V

C

I

I

N

R

P

P

R

A

A

Q

W

F

Y

W

A

E

E

D

N

K

G

A

I

V

T

E

L

M

H

K

T

L

G

G

D

A

P
x
E

V
|
V

V

V

A

S

I

L

D

D

R

P

A

A

K

H

T

T

V

V

T

K

S

L

A

G

N

D

G

G

V

S

V

A

V

I

P

E

Y

Y

D

G

K

K

L

L

L

I

L

W

S

A

T
|
T

G

G

S

G

K

D

P

P

L

R

M

R

P

L

P

S

L

C

P

V

G

G

L

A

D

D

L

L

P

A

G

G

V

V

V

H

A

A

F

V

R

R

D

T

I

K

A

E

D

D

V

A

E

D

K

R

M

L

L

M

D

A

A

E

A

L

E

D

A

A

-

G

K

A

Q

K

R

N

A

A

V

V

I

I

G

G

L

Y

L
x
I

G

G

L

L

E

E

A

A

W

A

G

V

L

L

K

T

R

K

R

F

G

G

M

V

P

N

V

V

A

T

L

L

V

L

H

E

L

A

M

L

P

P

T

R

L

V

M

L

E

A

R

R

Q

V

L

A

D

G

V

E

E

A

A

L

G

S

G

E

L

F

L

Y

Q

Q

K

A

D

E

L

H

T

R

E

A

R

H

G

G

L

V

H

D

F

L

F

R

T

T

S

G

G

A

Q

A

T

M

E

D

E

C

I

I

M

E

G

G

A

D

E

G

T

T

G

-

R

K

A

V

Q

T

G

G

V

V

K

R

L

M

T

Q

D

D

G

G

R

S

E

V

I

I

P

P

A

A

D

D

L

I

V

V

V

I

V

V

A

G

I

I

G

G

I

I

R

V

P

P

N

C

V

V

D

G

L

A

A

L

K

I

A

S

A

A

G

G

L

A

D

S

I

G

N

G

R

N

G

G

I

V

E

D

V

V

G

D

D

E

D

F

M

C

A

R

T

T

S

S

D

L

P

T

A

D

I

V

Y

Y

S

A

V

I

G
|
G

E
x
D

C

C

V

A

E

A

H

H

active site: R44 (= R48), P45 (≠ I49)
binding flavin-adenine dinucleotide: R36 (≠ A40), E37 (= E41), R44 (= R48), P45 (≠ I49), S48 (= S52), K49 (≠ S53), E81 (≠ P84), V82 (= V85), T109 (= T112), I157 (≠ L159), G278 (= G281), D279 (≠ E282)

Sites not aligning to the query:

active site: 13, 302
binding flavin-adenine dinucleotide: 9, 10, 12, 13, 14, 297, 298, 325, 326

4emiA Toluene dioxygenase reductase in reduced state in complex with NAD+ (see paper)
32% identity, 34% coverage: 9:283/819 of query aligns to 4:275/402 of 4emiA

query
sites
4emiA

L

V

V

A

V

I

I

I

G

G

N

N

G
|
G

M
x
V

A
x
G

G

G

M

F

R

T

T

T

V

A

E

Q

E

A

L

L

L

R

A

A

I

E

A

G

P

-

A

F

R

E

Y

G

D

R

I

I

T

S

V

L

F

I

G

G

A
x
D

E
|
E

P

P

H

H

P

L

N

P

Y

Y

N

D

R
|
R

I
x
P

M

S

L

L

S

S

-
x
K

S

A

V

V

L

L

A

D

G

G

E

S

K

L

Q

E

V

R

D

P

I

I

V

L

I

A

N

E

P

A

R

D

A

-

W

W

Y

Y

A

G

E

E

N

A

G

R

I

I

T

D

L

M

H

L

T

T

G

G

D

P

P
x
E

V
|
V

V

T

A

A

I

L

D

D

R

V

A

Q

A

T

K

R

T

T

V

I

T

S

S

L

A

D

N

D

G

G

V

T

V

L

P

S

Y

A

D

D

K

A

L

I

L

V

L

I

S

A

T
|
T

G
|
G

S

S

K

R

P

A

L
x
R

M

T

P

M

P

A

L

L

P

P

G

G

L

S

D

Q

L

L

P

P

G

G

V

V

A

T

F

L

R

R

D

T

I

Y

A

G

D

D

V

V

E

Q

K

V

M

L

L

R

D

D

A

S

A

W

E

T

A

S

K

A

Q

T

R

R

A

L

V

L

V

I

I

V

G
|
G

G

G

L
|
L

L
x
I

G

G

L

C

E
|
E

A

V

A

A

W

T

G

T

L

A

K

R

R

K

R

L

G

G

M

L

P

S

V

V

A

T

L

I

V

L

H
x
E

L
x
A

M

G

P

D

T

E

L

L

M

L

E

V

R
|
R

Q

V

L

L

D

G

V

R

E

R

A

I

G

G

G

A

L

W

L

L

Q

R

K

G

D

L

L

L

T

T

E

E

R

L

G

G

L

V

H

Q

-

V

-

E

-

L

-

G

-

T

-

G

-

V

-

G

F

F

F

S

T

G

S

E

G

G

Q

Q

T

L

E

E

E

Q

I

V

M

M

G

A

A

-

E

-

T

-

G

-

R

-

A

-

Q

-

G

-

V

-

K

-

L

-

T

S

D

D

G

G

R

R

E

S

I

F

P

V

A

A

D

D

L

S

V

A

V

L

V

I

A

C

I
x
V

G
|
G

I
x
A

R

E

P

P

N

A

V

D

D

Q

L

L

A

A

K

R

A

Q

A

A

G

G

L

L

D

A

I

C

N

D

R

R

G

G

I

V

E

I

V

V

G

D

D

H

D

C

M

G

A

A

T

T

S

L

D

A

P

K

A

G

I

V

Y

F

S

A

V

V

G
|
G

E
x
D

C

V

binding flavin-adenine dinucleotide: G10 (= G15), V11 (≠ M16), G12 (≠ A17), D34 (≠ A40), E35 (= E41), R42 (= R48), P43 (≠ I49), K47 (vs. gap), E78 (≠ P84), V79 (= V85), T106 (= T112), G107 (= G113), G273 (= G281), D274 (≠ E282)
binding nicotinamide-adenine-dinucleotide: R111 (≠ L117), G149 (= G155), L152 (= L158), I153 (≠ L159), E156 (= E162), E172 (≠ H178), A173 (≠ L179), R180 (= R186), V236 (≠ I244), G237 (= G245), A238 (≠ I246)

Sites not aligning to the query:

4emjA Complex between the reductase and ferredoxin components of toluene dioxygenase (see paper)
32% identity, 34% coverage: 9:283/819 of query aligns to 5:276/406 of 4emjA

query
sites
4emjA

L

V

V

A

V

I

I

I

G

G

N

N

G
|
G

M
x
V

A
x
G

G

G

M

F

R

T

T

T

V

A

E

Q

E

A

L

L

L

R

A

A

I

E

A

G

P

-

A

F

R

E

Y

G

D

R

I

I

T

S

V

L

F

I

G

G

A
x
D

E
|
E

P

P

H

H

P

L

N

P

Y

Y

N

D

R
|
R

I
x
P

M

S

L

L

S
|
S

-
x
K

S

A

V

V

L

L

A

D

G

G

E

S

K

L

Q

E

V

R

D

P

I

I

V

L

I

A

N

E

P

A

R

D

A

-

W

W

Y

Y

A

G

E

E

N

A

G

R

I

I

T

D

L

M

H

L

T

T

G

G

D

P

P

E

V
|
V

V

T

A

A

I

L

D

D

R

V

A

Q

A

T

K

R

T

T

V

I

T

S

S

L

A

D

N

D

G

G

V

T

V

L

P

S

Y

A

D

D

K

A

L

I

L

V

L

I

S

A

T
|
T

G
|
G

S

S

K

R

P

A

L

R

M

T

P

M

P

A

L

L

P

P

G

G

L

S

D

Q

L

L

P

P

G

G

V

V

A

T

F

L

R
|
R

D

T

I

Y

A

G

D

D

V

V

E

Q

K

V

M

L

L

R

D

D

A

S

A

W

E

T

A

S

K

A

Q

T

R

R

A

L

V

L

V

I

I

V

G

G

L

L

L

I

G

G

L

C

E

E

A

V

A

A

W

T

G

T

L

A

K

R

R

K

R

L

G

G

M

L

P

S

V

V

A

T

L

I

V

L

H

E

L

A

M

G

P

D

T

E

L

L

M

L

E

V

R

R

Q

V

L

L

D

G

V

R

E

R

A

I

G

G

G

A

L

W

L

L

Q

R

K

G

D

L

L

L

T

T

E

E

R

L

G

G

L

V

H

Q

-

V

-

E

-

L

-

G

-

T

-

G

-

V

-

G

F

F

F

S

T

G

S

E

G

G

Q

Q

T

L

E

E

E

Q

I

V

M

M

G

A

A

-

E

-

T

-

G

-

R

-

A

-

Q

-

G

-

V

-

K

-

L

-

T

S

D

D

G

G

R

R

E

S

I

F

P

V

A

A

D

D

L

S

V

A

V

L

V

I

A

C

I

V

G

G

I

A

R

E

P

P

N

A

V

D

D

Q

L

L

A

A

K

R

A

Q

A

A

G

G

L

L

D

A

I

C

N

D

R

R

G

G

I

V

E

I

V

V

G

D

D

H

D

C

M

G

A

A

T

T

S

L

D

A

P

K

A

G

I

V

Y

F

S

A

V

V

G
|
G

E
x
D

C

V

binding flavin-adenine dinucleotide: G11 (= G15), V12 (≠ M16), G13 (≠ A17), D35 (≠ A40), E36 (= E41), R43 (= R48), P44 (≠ I49), S47 (= S52), K48 (vs. gap), V80 (= V85), T107 (= T112), G108 (= G113), R128 (= R133), G274 (= G281), D275 (≠ E282)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 291, 292, 319, 320

8c0zE Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
28% identity, 45% coverage: 8:375/819 of query aligns to 2:376/424 of 8c0zE

query
sites
8c0zE

K

K

L

H

V

V

V

I

I

L

G
|
G

N

N

G

G

M
x
P

A

A

G

G

M

V

R

I

T

A

V

A

E

E

E

T

L

L

L

R

A

R

I

A

A

A

P

P

A

T

R

D

Y

-

D

D

I

I

T

L

V

L

F

F

G

G

A

S

E

E

P

D

H

A

P

P

N

P

Y

Y

N

S

R
|
R

I
x
M

M

A

L

I

S
x
P

S

Y

V

L

L

L

A

E

G

G

E

N

K

I

Q

D

V

E

D

S

D

G

I

T

V

W

I

L

N

R

P

K

R

S

A

P

W

G

Y

H

A

F

E

D

N

R

G

L

I

R

T

I

L

H

H

E

T

M

G

R

D

G

P

R

V

A

V

V

A

S

I

L

D

D

R

S

A

E

A

R

K

R

T

R

V

I

T

L

S

F

A

D

N

D

G

G

V

H

V

F

V

E

P

S

Y

W

D

D

K

R

L

L

L

I

S

A

T
|
T

G

G

S

S

K

H

P

P

L

V

M

R

P

P

L

I

P

P

G

G

L

I

D

D

L

L

P

P

G

E

V

V

V

Q

A

T

F

C

R
x
W

D

T

I

L

A

E

D

D

V

A

E

R

K

A

M

I

L

A

D

R

A

F

A

A

E

T

A

P

K

G

Q

A

R

R

A

V

V

L

V

Q

I

L

G

G

G

A

G

G

L
x
F

L

I

G

G

L

C

E

I

A

I

A

M

W

E

G

A

L

L

K

A

R

A

R

R

G

G

M

V

P

E

V

L

A

T

L

V

V

V

H

E

L

M

M

G

P

D

T

R

L

M

M

V

E

P

R

R

Q

M

L

M

D

T

V

P

E

T

A

A

G

G

L

M

L

I

Q

R

K

K

D

W

L

V

T

E

E

D

R

Q

G

G

L

V

H

R

F

V

T

T

S

N

G

A

Q

G

T

V

E

S

E

R

I

I

-

D

M

C

G

R

A

A

E

S

T

N

G

D

R

A

A

P

Q

L

G

D

V

V

K

T

L

L

T

S

D

T

G

G

R

E

E

V

I

V

P

V

A

A

D

D

L

L

V

V

V

I

V

V

A

A

I

A

G

G

I

V

R

A

P

P

N

N

V

I

D

A

L

F

A

L

K

E

A

A

A

T

G

P

L

V

D

H

I

V

N

A

R

K

G

G

I

V

E

L

V

V

G

D

D

D

D

R

M

L

A

Q

T

T

S

S

D

V

P

P

A

G

I

I

Y

F

S

A

V

A

G

G

E
x
D

C

V

V

A

E

E

H

A

R

P

G

D

Q

L

I

F

F

T

G

G

-

A

-

H

L

L

V

V

A

A

P

A

I

I

W

Q

-

P

-

N

-

A

E

D

Q

Q

A

A

K

R

V

V

C

A

A

A

S

L

R

N

L

M

A

A

G

G

R

H

D

-

D

E

A

A

H

R

Y

L

E

K

T

G

P

V

P

L

L

A

S

I

T

N

R

V

L

L

K

D

I

T

T

L

G

G

I

L

D

I

V

S

F

S

S

S

A

F

G

G

Q

Q

L

W

A

W

A

G

Q

E

D

E

-

R

-

E

-

R

-

G

-

A

-

G

-

V

E

E

A

H

D

V

E

D

E

E

L

A

V

A

Y

Y

R

R

D

-

S

-

A

-

R

-

G

-

I

-

Y

Y

K

L

K

S

L

L

V

Q

I

F

R

K

A

D

D

D

K

V

V

L

V

I

G

G

A

A

V

T

M

S

Y

I

G

G

binding flavin-adenine dinucleotide: G7 (= G13), P10 (≠ M16), R41 (= R48), M42 (≠ I49), P45 (≠ S52), T105 (= T112), W126 (≠ R133), F151 (≠ L158), D276 (≠ E282)

8a56B Coenzyme a-persulfide reductase (coapr) from enterococcus faecalis (see paper)
30% identity, 37% coverage: 8:310/819 of query aligns to 2:313/539 of 8a56B

query
sites
8a56B

K

K

L

I

V

V

V

I

I
|
I

G
|
G

N

G

G
x
V

M

A

A
x
G

G

G

M

M

R

S

T

A

V

A

E
x
T

E
x
R

L

L

L

R

A
x
R

I

L

A

M

P

-

A

E

R

D

Y

A

D

E

I

I

T

V

V

V

F

M

G
x
E

A
x
K

E

G

P

P

H

F

P

V

N

S

Y

F

N

A

R

N

I
x
C

M

G

L

L

S

P

S

Y

V

Y

L

V

A

S

G

G

E

E

-

I

-

A

K

E

Q

R

V

E

D

Q

D

L

I

L

V

V

I

Q

N

T

P

P

R

E

A

A

W

L

Y

K

A

A

E

R

N
x
F

G

N

I

L

T

D

L

V

H

R

T

P

G

H

D

H

P

E

V
|
V

V

V

A

A

I

I

D

D

R

P

A

I

A

E

K

K

T

V

V

I

T

T

S

V

A

K

N

H

G

E

V

T

V

E

V

I

-

L

-

T

-

E

P

H

Y

Y

D

D

K

K

L

L

L

I

L

L

S
|
S

T
x
P

G
|
G

S

A

K

K

P

P

L

F

M

V

P

P

L

I

P

T

G

G

L

L

-

A

D

E

L

A

P

K

G

N

V

V

V

F

A

S

F

L

R

R

D

N

I

V

A

P

D

D

V

L

E

D

K

Q

M

I

L

M

D

T

A

A

A

L

E

T

A

P

K

E

-

T

Q

K

R

R

A

A

V

V

I

I

G

G

G

A

G

G

L

F

L
x
I

G

G

L

L

E

E

A

M

A

A

W

E

G

N

L

L

K

Q

R

K

R

R

G

G

M

L

P

E

V

V

A

T

L

L

V

V

H

E

L

K

M

A

P

P

T

H

L

V

M

L

E

P

R

-

Q

P

L

L

D

D

V

E

E

E

A

M

G

A

L

F

L

V

Q

K

K

A

D

E

L

L

T

S

E

K

R

N

G

N

L

V

H

Q

F

V

F

I

T

T

S

-

G

G

Q

Q

T

S

E

A

E

V

I

A

M

F

G

E

A

E

E

E

T

-

G

-

R

-

A

G

Q

Q

G

V

V

I

K

R

L

L

T

E

D

D

G

G

R

Q

E

T

I

L

P

A

A

S

D

D

L

L

V

T

V

I

V

L

A

S

I

V

G

G

I

V

R

Q

P

P

N

E

V

N

D

T

L

L

A

A

K

V

A

E

A

A

G

G

L

V

-

A

-

T

D

G

I

L

N

R

R

G

G

G

I

I

E

V

V

V

G

D

D

E

D

H

M

Y

A

Q

T

T

S

N

D

Q

P

P

A

D

I

I

Y

Y

S

A

V

V

G
|
G

E
x
D

C

A

V

I

E

V

H

V

R

K

G

Q

Q

Q

I

I

-

T

-

Q

-

E

-

D

-

A

-

L

F

I

G
x
S

L
|
L

V
x
A

A

S

P

P

I

A

W

N

E

R

Q

Q

A

G

K
x
R

V

Q

C

V

A

A

S

D

R

V

L

I

A

A

G

G

binding flavin-adenine dinucleotide: I6 (= I12), G7 (= G13), V9 (≠ G15), G11 (≠ A17), E32 (≠ G39), K33 (≠ A40), C42 (≠ I49), V80 (= V85), S109 (= S111), P110 (≠ T112), G111 (= G113), I159 (≠ L159), G278 (= G281), D279 (≠ E282), S295 (≠ G292), L296 (= L293), A297 (≠ V294)
binding 3'-phosphate-adenosine-5'-diphosphate: T17 (≠ E23), R18 (≠ E24), R21 (≠ A27), F70 (≠ N75), R305 (≠ K302)

Sites not aligning to the query:

P16640 Putidaredoxin reductase CamA; Pdr; Putidaredoxin--NAD(+) reductase; EC 1.18.1.5 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
31% identity, 38% coverage: 1:314/819 of query aligns to 1:325/422 of P16640

query
sites
P16640

M

M

N

N

A

A

L

-

P

-

R

N

Q

D

K

N

L

V

V

V

V

I

I

V

G

G

N

T

G

G

M

L

A
|
A

G

G

M

V

R

E

T

V

V

A

E

F

E

G

L

L

L

R

A

A

I

S

A

G

P

W

A

E

R

G

Y

N

D

I

I

R

T

L

V

V

F

G

G
x
D

A

A

E

T

P

V

H

I

P

P

N

H

Y

H

N

L

R

P

I

P

M

L

L

S

S
x
K

S

A

V

Y

L

L

A

A

G

G

E

K

K

A

Q

T

V

A

D

E

D

S

I

L

V

Y

I

L

N

R

P

T

R

P

A

D

W

A

Y

Y

A

A

E

A

N

Q

G

N

I

I

T

Q

L

L

H

L

T

G

G

G

D

T

P

Q

V
|
V

V

T

A

A

I

I

D

N

R

R

A

D

A

R

K

Q

T

Q

V

V

T

I

S

L

A

S

N

D

G

G

V

R

V

A

V

L

P

D

Y

Y

D

D

K

R

L

L

L

V

L

L

S

A

T

T

G

G

S

G

K

R

P

P

L

R

M

-

P

-

P

P

L

L

P

P

G

V

L

A

D

S

L

-

P

-

G

G

V

A

V

V

A

G

-

K

-

A

-

N

-

F

-

R

-

Y

F

L

R
|
R

D

T

I

L

A

E

D

D

V

A

E

E

K

C

M

I

L

R

D

R

A

Q

A

L

E

I

A

A

K

D

Q

N

R

R

A

L

V

V

I

I

G

G

L

Y

L

I

G

G

L

L

E

E

A

V

A

A

W

A

G

T

L

A

K

I

R

K

R

A

G

N

M

M

P

H

V

V

A

T

L

L

V

L

H

D

L

T

M

A

P

A

T

R

L

V

M

L

E

E

R

R

Q

V

L

T

D

A

V

P

E

P

A

V

G

S

G

A

L

F

L

Y

Q

E

K

H

D

L

L

H

T

R

E

E

R

A

G

G

L

V

H

D

F

I

F

R

T

T

S

G

G

T

Q

Q

T

V

E

C

E

G

I

F

-

E

M

M

G

S

A

T

E

D

T

Q

G

Q

R

K

A

V

Q

T

G

A

V

V

K

L

L

C

T

E

D

D

G

G

R

T

E

R

I

L

P

P

A

A

D

D

L

L

V

V

V

I

V

A

A

G

I

I

G

G

I

L

R

I

P

P

N

N

V

C

D

E

L

L

A

A

K

S

A

A

G

G

L

L

D

Q

I

V

N

D

R

N

G

G

I

I

E

V

V

I

G

N

D

E

D

H

M

M

A

Q

T

T

S

S

D

D

P

P

A

L

I

I

Y

M

S

A

V

V

G

G

E
x
D

C

C

V

A

E

R

H

F

R

H

G

S

Q

Q

I

L

F

Y

G

D

L

R

-

W

-

V

-

R

-

I

-

E

-

S

V
|
V

A

P

P

N

I

A

W

L

E

E

Q

Q

A

A

K

R

V

K

C

I

A

A

S

A

R

I

L

L

A

C

G

G

-

K

-

V

-

P

R

R

D

D

D

E

A

A

A15 (= A17) binding
D37 (≠ G39) binding
K50 (≠ S52) binding
V83 (= V85) binding
R134 (= R133) binding
D284 (≠ E282) binding
V302 (= V294) binding

1q1wA Crystal structure of putidaredoxin reductase from pseudomonas putida (see paper)
31% identity, 37% coverage: 8:314/819 of query aligns to 5:324/422 of 1q1wA

query
sites
1q1wA

K

N

L

V

V

V

V

I

I

V

G
|
G

N

T

G
|
G

M
x
L

A
|
A

G

G

M

V

R

E

T

V

V

A

E

F

E

G

L

L

L

R

A

A

I

S

A

G

P

W

A

E

R

G

Y

N

D

I

I

R

T

L

V

V

F
x
G

G
x
D

A

A

E

T

P

V

H

I

P

P

N

H

Y

H

N
x
L

R
x
P

I

P

M

L

L

S

S
x
K

S

A

V

Y

L

L

A

A

G

G

E

K

K

A

Q

T

V

A

D

E

D

S

I

L

V

Y

I

L

N

R

P

T

R

P

A

D

W

A

Y

Y

A

A

E

A

N

Q

G

N

I

I

T

Q

L

L

H

L

T

G

G

G

D

T

P

Q

V
|
V

V

T

A

A

I

I

D

N

R

R

A

D

A

R

K

Q

T

Q

V

V

T

I

S

L

A

S

N

D

G

G

V

R

V

A

V

L

P

D

Y

Y

D

D

K

R

L

L

L

V

L

L

S
x
A

T
|
T

G
|
G

S

G

K

R

P

P

L

R

M

-

P

-

P

P

L

L

P

P

G

V

L

A

D

S

L

-

P

-

G

G

V

A

V

V

A

G

-

K

-

A

-

N

-

F

-

R

-

Y

F

L

R
|
R

D

T

I

L

A

E

D

D

V

A

E

E

K

C

M

I

L

R

D

R

A

Q

A

L

E

I

A

A

K

D

Q

N

R

R

A

L

V

V

I

I

G

G

L

Y

L
x
I

G

G

L

L

E

E

A

V

A

A

W

A

G

T

L

A

K

I

R

K

R

A

G

N

M

M

P

H

V

V

A

T

L

L

V

L

H

D

L

T

M

A

P

A

T

R

L

V

M

L

E

E

R

R

Q

V

L

T

D

A

V

P

E

P

A

V

G

S

G

A

L

F

L

Y

Q

E

K

H

D

L

L

H

T

R

E

E

R

A

G

G

L

V

H

D

F

I

F

R

T

T

S

G

G

T

Q

Q

T

V

E

C

E

G

I

F

-

E

M

M

G

S

A

T

E

D

T

Q

G

Q

R

K

A

V

Q

T

G

A

V

V

K

L

L

C

T

E

D

D

G

G

R

T

E

R

I

L

P

P

A

A

D

D

L

L

V

V

V

I

V

A

A

G

I

I

G

G

I

L

R

I

P

P

N

N

V

C

D

E

L

L

A

A

K

S

A

A

G

G

L

L

D

Q

I

V

N

D

R

N

G

G

I

I

E

V

V

I

G

N

D

E

D

H

M

M

A

Q

T

T

S

S

D

D

P

P

A

L

I

I

Y

M

S

A

V

V

G

G

E
x
D

C

C

V

A

E

R

H

F

R

H

G

S

Q

Q

I

L

F

Y

G

D

L

R

-

W

-

V

-

R

-

I

-

E

-
x
S

V
|
V

A

P

P

N

I

A

W
x
L

E

E

Q

Q

A

A

K

R

V

K

C

I

A

A

S

A

R

I

L

L

A

C

G

G

-

K

-

V

-

P

R

R

D

D

D

E

A

A

active site: L13 (≠ M16), L44 (≠ N47), P45 (≠ R48), L305 (≠ W298)
binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), L13 (≠ M16), A14 (= A17), G35 (≠ F38), D36 (≠ G39), L44 (≠ N47), P45 (≠ R48), K49 (≠ S52), V82 (= V85), A108 (≠ S111), T109 (= T112), G110 (= G113), R133 (= R133), I159 (≠ L159), D283 (≠ E282), S300 (vs. gap), V301 (= V294)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 329

2v3aA Crystal structure of rubredoxin reductase from pseudomonas aeruginosa. (see paper)
30% identity, 37% coverage: 6:310/819 of query aligns to 1:302/381 of 2v3aA

query
sites
2v3aA

R

R

Q

A

K

P

L

L

V

V

V

I

I
|
I

G
|
G

N
x
T

G
|
G

M
x
L

A
|
A

G

G

M

Y

R

N

T

L

V

A

E

R

E

E

L

W

L

R

A

K

I

L

A

D

P

-

A

G

R

E

Y

T

D

P

I

L

T

L

V

M

F

I

G
x
T

A
|
A

E
x
D

P

D

H

G

P

R

N

S

Y

Y

N

S

R
x
K

I
x
P

M

M

L

L

S

S

S

T

V

G

L

F

A

S

G

K

E

N

K

K

Q

D

V

A

D

D

D

G

I

L

V

A

I

M

-

A

N

E

P

P

R

G

A

A

W

M

Y

A

A

E

E

Q

N

L

G

N

I

A

T

R

L

I

H

L

T

T

G

H

D

T

P
x
R

V
|
V

V

T

A

G

I

I

D

D

R

P

A

G

A

H

K

Q

T

R

V

I

T

W

S

I

A

G

N

E

G

E

V

E

V

V

V

R

P

-

Y

Y

D

R

K

D

L

L

L

V

L

L

S
x
A

T
x
W

G
|
G

S

A

K

E

P

P

L

I

M

R

P

V

P

P

L

V

P

E

G

G

L

D

D

A

L

Q

P

D

G

A

V

L

V

Y

A

P

F

I

R

N

D

D

I

L

A

E

D

D

V

Y

E

A

K

R

M

F

L

R

D

Q

A

A

E

A

A

G

K

K

Q

R

R

R

A

V

V

L

I

L

G

G

G

A

G

G

L

L

L
x
I

G

G

L

C

E

E

A
x
F

A

A

W

N

G

D

L

L

K

S

R

S

R

G

G

G

M

Y

P

Q

V

L

A

D

L

V

V

V

H

A

L

P

M

C

P

E

T

Q

L

V

M

M

E

P

R

G

Q

L

L

L

D

H

V

P

E

A

A

A

G

A

G

K

L

A

L

V

Q

Q

K

A

D

G

L

L

T

E

E

G

R

L

G

G

L

V

H

R

F

F

F

H

T

L

S

G

G

P

Q

V

T

L

E

A

E

S

I

L

M

K

G

K

A

A

E

-

T

-

G

G

R

E

A

G

Q

L

G

E

V

A

K

H

L

L

T

S

D

D

G

G

R

E

E

V

I

I

P

P

A

C

D

D

L

L

V

V

V

S

A

A

I

V

G

G

I

L

R

R

P

P

N

R

V

T

D

E

L

L

A

A

K

F

A

A

G

G

L

L

D

A

I

V

N

N

R

R

G

G

I

I

E

V

V

V

G

D

D

R

D

S

M

L

A

R

T

T

S

S

D

H

P

A

A

N

I

I

Y

Y

S

A

V

L

G

G

E
x
D

C

C

V

A

E

E

H

V

R

D

G

G

Q

L

I

N

F

L

G
x
L

L
x
Y

V
|
V

A

M

P

P

I

L

W
x
M

E

A

Q

C

A

A

K

R

V

A

C

L

A

A

S

Q

R

T

L

L

A

A

G

G

active site: L11 (≠ M16), K42 (≠ R48), P43 (≠ I49), M290 (≠ W298)
binding flavin-adenine dinucleotide: I7 (= I12), G8 (= G13), T9 (≠ N14), G10 (= G15), A12 (= A17), T33 (≠ G39), A34 (= A40), D35 (≠ E41), K42 (≠ R48), P43 (≠ I49), R79 (≠ P84), V80 (= V85), A105 (≠ S111), W106 (≠ T112), G107 (= G113), I153 (≠ L159), F157 (≠ A163), D274 (≠ E282), L284 (≠ G292), Y285 (≠ L293), V286 (= V294)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 317

Q9HTK9 Rubredoxin-NAD(+) reductase; RdxR; EC 1.18.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
30% identity, 37% coverage: 6:310/819 of query aligns to 4:305/384 of Q9HTK9

query
sites
Q9HTK9

R

R

Q

A

K

P

L

L

V

V

V

I

I

I

G

G

N
x
T

G
|
G

M
x
L

A
|
A

G

G

M

Y

R

N

T

L

V

A

E

R

E

E

L

W

L

R

A

K

I

L

A

D

P

-

A

G

R

E

Y

T

D

P

I

L

T

L

V

M

F

I

G
x
T

A
|
A

E

D

P

D

H

G

P

R

N

S

Y

Y

N

S

R
x
K

I

P

M

M

L

L

S

S

S

T

V

G

L

F

A

S

G

K

E

N

K

K

Q

D

V

A

D

D

D

G

I

L

V

A

I

M

-

A

N

E

P

P

R

G

A

A

W

M

Y

A

A

E

E

Q

N

L

G

N

I

A

T

R

L

I

H

L

T

T

G

H

D

T

P

R

V
|
V

V

T

A

G

I

I

D

D

R

P

A

G

A

H

K

Q

T

R

V

I

T

W

S

I

A

G

N

E

G

E

V

E

V

V

V

R

P

-

Y

Y

D

R

K

D

L

L

L

V

L

L

S

A

T

W

G

G

S

A

K

E

P

P

L

I

M

R

P

V

P

P

L

V

P

E

G

G

L

D

D

A

L

Q

P

D

G

A

V

L

V

Y

A

P

F

I

R

N

D

D

I

L

A

E

D

D

V

Y

E

A

K

R

M

F

L

R

D

Q

A

A

E

A

A

G

K

K

Q

R

R

R

A

V

V

L

I

L

G

G

G

A

G

G

L

L

L

I

G

G

L

C

E
|
E

A

F

A

A

W

N

G

D

L

L

K

S

R

S

R

G

G

G

M

Y

P

Q

V

L

A

D

L

V

V

V

H

A

L

P

M

C

P

E

T

Q

L

V

M

M

E

P

R

G

Q

L

L

L

D

H

V

P

E

A

A

A

G

A

G

K

L

A

L

V

Q

Q

K

A

D

G

L

L

T

E

E

G

R

L

G

G

L

V

H

R

F

F

F

H

T

L

S

G

G

P

Q

V

T

L

E

A

E

S

I

L

M

K

G

K

A

A

E

-

T

-

G

G

R

E

A

G

Q

L

G

E

V

A

K

H

L

L

T

S

D

D

G

G

R

E

E

V

I

I

P

P

A

C

D

D

L

L

V

V

V

S

A

A

I

V

G

G

I

L

R

R

P

P

N

R

V

T

D

E

L

L

A

A

K

F

A

A

G

G

L

L

D

A

I

V

N

N

R

R

G

G

I

I

E

V

V

V

G

D

D

R

D

S

M

L

A

R

T

T

S

S

D

H

P

A

A

N

I

I

Y

Y

S

A

V

L

G

G

E
x
D

C

C

V

A

E

E

H

V

R

D

G

G

Q

L

I

N

F

L

G

L

L

Y

V
|
V

A

M

P

P

I

L

W

M

E

A

Q

C

A

A

K

R

V

A

C

L

A

A

S

Q

R

T

L

L

A

A

G

G

TGLA 12:15 (≠ NGMA 14:17) binding
TA 36:37 (≠ GA 39:40) binding
K45 (≠ R48) binding
V83 (= V85) binding
E159 (= E162) binding
D277 (≠ E282) binding
V289 (= V294) binding

Sites not aligning to the query:

320 binding

Q9LK94 Monodehydroascorbate reductase 4, peroxisomal; AtMDAR4; EC 1.6.5.4 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 28% coverage: 62:292/819 of query aligns to 72:306/488 of Q9LK94

query
sites
Q9LK94

D

N

D

D

I

E

V

K

I

L

N

T

P

P

R

K

A

-

W

W

Y

Y

A

K

E

D

N

H

G

G

I

I

T

E

L

L

H

V

T

L

G

G

D

T

P

R

V

V

V

K

A

S

I

V

D

D

R

V

A

R

K

K

T

T

V

L

T

L

S

S

A

S

N

T

G

G

V

E

V

T

V

I

P

S

Y

Y

D

K

K

F

L

L

L

I

S

A

T

T

G

G

S

A

K

R

P

A

L

L

M

K

P

L

P

E

-

E

-

F

-

G

L

V

P

E

G

G

L

S

D

D

L

A

P

E

G

N

V

V

V

C

A

Y

F

L

R

R

D

D

I

L

A

A

D

D

V

A

E

N

K

R

M

L

L

A

D

T

A

V

A

I

E

Q

A

S

K

S

Q

S

R

N

-

G

-

N

A

A

V

V

I

I

G

G

L

Y

L

I

G

G

L

M

E

E

A

C

A

A

W

A

G

S

L

L

K

V

R

I

R

N

G

K

M

I

P

N

V

V

A

T

L

M

V

V

H

F

L

P

M

E

P

A

T

H

L

C

M

M

E

A

R

R

Q

L

L

F

D

T

V

P

E

K

A

I

G

A

G

S

L

L

L

Y

Q

E

K

D

D

Y

L

Y

T

R

E

A

R

K

G

G

L

V

H

K

F

F

F

I

T

K

S

G

G

T

Q

V

T

L

E

T

E

S

I

F

M

E

G

F

A

D

E

S

T

N

G

K

R

K

A

V

Q

T

G

A

V

V

K

N

L

L

T

K

D

D

G

G

R

S

E

H

I

L

P

P

A

A

D

D

L

L

V

V

A

G

I

I

G

G

I

I

R

R

P

P

N

N

V

T

D

S

L

L

A

F

K

E

A

G

A

Q

G

-

L

L

D

T

I

I

N

E

R

K

-

G

G

G

I

I

E

K

V

V

G

N

D

S

D

R

M

M

A

Q

T

S

S

S

D

D

P

S

A

S

I

V

Y

Y

S

A

V

I

G

G

E

D

C

V

V

A

E

T

H

F

R

P

G

V

Q

K

I

L

F

F

G

G

Sites not aligning to the query:

11 G→Q: In sdp2-2; loss of ascorbate recycling.
14 V→A: In sdp2-1; loss of ascorbate recycling.
386 G→Q: In sdp2-3; loss of ascorbate recycling.
483:488 mutation Missing: Loss of peroxisomal targeting.
488 mutation Missing: No effect on peroxisomal targeting.

Query Sequence

>WP_011382976.1 NCBI__GCF_000009985.1:WP_011382976.1
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQ
VDDIVINPRAWYAENGITLHTGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPP
LPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLGLEAAWGLKRRGMPVALVHLM
PTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQ
AKVCASRLAGRDDAHYETPPLSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKK
LVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRMIFGQAYADTTCKGHAGGVNV
AATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYL
LCAWPSEYVDDYQSRFINERAHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIP
TVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAKGLRTVKTCVGSEWCRFGTQD
STGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGR
RAAFARFVFSQNYAQIDPWAERASGEVDAHEFKTLAEVG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory