SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011383255.1 NCBI__GCF_000009985.1:WP_011383255.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
44% identity, 99% coverage: 6:388/388 of query aligns to 11:392/393 of 2ordA

query
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2ordA

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active site: F134 (= F130), E186 (= E182), D219 (= D215), Q222 (= Q218), K248 (= K244), T276 (= T273), R367 (= R363)
binding pyridoxal-5'-phosphate: G102 (= G97), T103 (≠ A98), F134 (= F130), H135 (= H131), E186 (= E182), D219 (= D215), V221 (= V217), Q222 (= Q218), K248 (= K244)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
44% identity, 99% coverage: 6:388/388 of query aligns to 3:384/385 of Q9X2A5

query
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Q9X2A5

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GT 94:95 (≠ GA 97:98) binding pyridoxal 5'-phosphate
T268 (= T273) binding pyridoxal 5'-phosphate

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
44% identity, 99% coverage: 2:387/388 of query aligns to 8:396/400 of 4addA

query
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4addA

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active site: F136 (= F130), E188 (= E182), D221 (= D215), Q224 (= Q218), K250 (= K244), T279 (= T273), R372 (= R363)
binding pyridoxal-5'-phosphate: G103 (= G97), A104 (= A98), F136 (= F130), H137 (= H131), D221 (= D215), V223 (= V217), K250 (= K244)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (≠ F10), F136 (= F130), R139 (= R133)

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
44% identity, 99% coverage: 2:387/388 of query aligns to 8:396/401 of 4adbB

query
sites
4adbB

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A

V

F

F

C

C

N

N

T
x
S

G
|
G

A
|
A

E

E

A

A

M

N

E

E

L

A

V

A

I

L

K

K

I

L

A

A

R

R

R

K

Y

F

H

A

H

H

C

D

A

R

G

Y

R

G

P

S

E

H

R

K

N

S

R

G

I

I

V

V

A

A

C

F

T

K

G

N

S

A

F
|
F

H
|
H

G

G

R

R

T

T

I

L

A

F

T

T

L

V

A

S

A

A

A

G

G

G

T

Q

P

P

K

A

Y

Y

L

S

E

Q

G

D

F

F

G

A

P

P

V

L

A

P

Q

A

G

D

F

I

D

R

H

H

V

A

P

A

Y

Y

G

N

D

D

L

I

E

N

A

S

A

A

R

S

G

A

A

L

I

I

G

D

S

D

N

S

T

T

A

C

A

A

L

V

L

I

V

V

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

I

V

R

V

P

P

A

A

D

S

P

N

A

A

Y

F

L

L

R

Q

G

G

L

L

R

R

A

E

L

L

A

C

D

N

Q

R

F

H

G

N

L

A

L

L

L

I

M

F

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

I

V

G

G

R

R

T

T

G

G

K

E

L

L

F

Y

A

A

H

Y

E

M

W

H

S

Y

G

G

I

V

A

T

P

P

D

D

V

L

M

L

G

T

L

T

A

A

K
|
K

G

A

L

L

G

G

F

F

P

P

V

V

G

G

A

A

V

L

L

L

A

A

T

T

E

E

K

E

A

C

A

A

S

R

C

V

M

M

T

T

P

V

G

G

T

T

H

H

G

G

C

T

T
|
T

F

Y

G

G

N

N

P

P

L

L

A

A

M

S

A

A

V

V

A

A

E

G

A

K

V

V

L

L

D

E

E

L

V

I

M

N

A

T

P

P

G

E

F

M

L

L

E

N

R

G

V

V

Q

K

A

Q

V

R

A

H

A

D

L

W

L

F

R

V

G

E

R

R

L

L

D

N

D

T

L

I

A

N

R

H

R

R

Y

Y

P

-

G

G

A

L

I

F

A

S

Q

E

V

V

R

R

G

G

Q

L

G

G

L

L

M

L

L

I

G

G

L

-

K

-

T

-

V

C

P

V

V

L

N

N

T

A

E

D

F

Y

-

A

-

G

N

Q

A

A

K

K

L

Q

F

I

A

S

-

Q

-

E

-

A

-

K

A

A

G

G

L

V

L

M

A

V

V

L

G

I

A

A

G

G

D

G

N

N

V

V

R
|
R

L

F

V

A

P

P

P

A

L

L

I

N

I

V

G

S

E

E

A

E

E

E

V

V

E

T

R

T

A

G

V

L

E

D

I

R

I

F

D

A

G

A

V

A

C

C

R

E

active site: F136 (= F130), E188 (= E182), D221 (= D215), Q224 (= Q218), K250 (= K244), T279 (= T273), R372 (= R363)
binding pyridoxal-5'-phosphate: S102 (≠ T96), G103 (= G97), A104 (= A98), F136 (= F130), H137 (= H131), D221 (= D215), V223 (= V217), Q224 (= Q218), K250 (= K244)

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
43% identity, 98% coverage: 2:382/388 of query aligns to 31:421/429 of P73133

query
sites
P73133

F

F

P

D

D

T

N

Y

L

V

L

M

P

N

V

T

F
x
Y

D

G

R

R

T

F

D

P

V

I

I

A

F

I

E

A

Q

R

G

G

E

Q

G

G

A

S

W

T

L

L

T

W

A

D

S

T

D

E

G

G

R

K

R

S

F

Y

L

L

D

D

F

F

G

V

A

A

G

G

V

I

A

A

V

T

N

C

A

T

L

L

G

G

H

H

A

A

H

H

P

P

R

A

L

L

V

V

A

R

A

A

L

V

E

S

A

D

Q

Q

A

I

G

Q

K

K

L

L

W

H

H

H

C

V

S

S

N

N

L

L

Y

Y

R

Y

V

I

P

P

G

E

Q

Q

E

G

R

E

V

L

A

A

E

K

R

W

L

I

I

V

K

E

A

H

S

S

F

C

A

A

D

D

T

R

A

V

F

F

C

C

N

N

T
x
S

G
|
G

A
|
A

E

E

A

A

M

N

E

E

L

A

V

A

I

I

K

K

I

L

A

V

R

R

R

K

Y

Y

H

A

H

H

C

T

A

V

-

L

G

D

R

F

P

L

E

E

R

Q

N

P

R

V

I

I

V

L

A

T

C

A

T

K

G

A

S

S

F

F

H

H

G

G

R
|
R

T

T

I

L

A

A

T

T

L

I

A

T

A

A

A

T

G

G

T

Q

P

P

K

K

Y

Y

L

Q

E

Q

G

Y

F

F

G

D

P

P

V

L

A

V

Q

P

G

G

F

F

D

D

H

Y

V

V

P

P

Y

Y

G

N

D

D

L

I

E

R

A

S

A

L

R

E

G

N

A

K

I

V

-

A

-

D

-

L

-

D

-

E

-

G

G

N

S

S

N

R

T

V

A

A

L

I

L

F

V

L

E

E

P

P

V

L

Q

Q

G

G

E
|
E

G

G

I

V

R

R

P

P

A

G

D

D

P

L

A

A

Y

Y

L

F

R

K

G

R

L

V

R

R

A

E

L

I

A

C

D

D

Q

Q

F

N

G

D

L

I

L

L

L

V

M

F

D
|
D

E

E

V

V

Q
|
Q

T

V

G

G

I

V

G

G

R

R

T

T

G

G

K

K

L

L

F

W

A

G

H

Y

E

E

W

H

S

L

G

G

I

V

A

E

P

P

D

D

V

I

M

F

G

T

L

S

A

A

K
|
K

G

G

L

L

G

A

G

G

F

V

P

P

V

I

G

G

A

A

V

M

L

M

A

C

T

-

E

K

K

K

A

F

A

C

S

D

C

V

M

F

T

E

P

P

G

G

T

N

H

H

G

A

C

S

T
|
T

F

F

G

G

N

N

P

P

L

L

A

A

M

C

A

A

V

A

A

G

E

L

A

A

V

V

L

L

D

K

E

T

V

I

M

E

A

G

P

D

G

R

F

L

L

L

E

D

R

N

V

V

Q

Q

A

A

V

R

A

G

A

E

L

Q

L

L

R

R

G

S

R

G

L

L

D

A

D

E

L

I

A

K

R

N

R

Q

Y

Y

P

P

G

T

A

L

I

F

A

T

Q

E

V

V

R

R

G

G

Q

W

G

G

L

L

M

I

L

N

G

G

L

L

K

E

T

I

V

S

P

A

V

E

N

S

T

S

E

L

F

T

N

S

A

V

K

E

L

I

F

V

A

K

A

A

-

A

-

M

-

E

-

Q

G

G

L

L

A

L

V

A

G

P

A

A

G

G

D

P

N

K

V

V

V

L

R
|
R

L

F

V

V

P

P

L

L

I

V

G

T

E

E

A

A

E

E

V

I

E

A

R

Q

A

A

V

V

E

E

I

I

I

L

Y39 (≠ F10) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (≠ T96) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G97) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (= A98) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R133) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E187) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D215) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q218) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K244) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T273) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R363) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
40% identity, 97% coverage: 6:382/388 of query aligns to 3:371/375 of 2eh6A

query
sites
2eh6A

L

L

L

M

P

N

V

N

F

Y

D

A

R

R

T

L

D

P

V

V

I

K

F

F

E

V

Q

R

G

G

E

K

G

G

A

V

W

Y

L

L

T

Y

A

D

S

E

D

E

G

G

R

K

R

E

F

Y

L

L

D

D

F

F

G

V

A

S

G

G

V

I

A

G

V

V

N

N

A

S

L

L

G

G

H

H

A

A

H

Y

P

P

R

K

L

L

V

T

A

E

A

A

L

L

E

K

A

E

Q

Q

A

V

G

E

K

K

L

L

W

L

H

H

C

V

S

S

N

N

L

L

Y

Y

R

E

V

N

P

P

G

W

Q

Q

E

E

R

E

V

L

A

A

E

H

R

K

L

L

I

V

K

K

A

H

S

F

F

W

A

T

D

E

-

G

T

K

A

V

F

F

C

A

N

N

T

S

G
|
G

A
x
T

E

E

A

S

M

V

E

E

L

A

V

A

I

I

K

K

I

L

A

A

R

R

R

K

Y

Y

H

W

H

R

C

D

A

K

G

G

R

K

P

-

E

N

R

K

N

W

R

K

I

F

V

I

A

S

C

F

T

E

G

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

I

Y

A

G

T

S

L

L

A

S

A

A

A

T

G

G

T

Q

P

P

K

K

Y

F

L

H

E

K

G

G

F

F

G

E

P

P

V

L

A

V

Q

P

G

G

F

F

D

S

H

Y

V

A

P

K

Y

L

G

N

D

D

L

I

E

D

A

S

A

V

R

Y

G

K

A

L

I

L

G

D

S

E

N

E

T

T

A

A

A

G

L

I

V

I

E
|
E

P

V

V

I

Q

Q

G

G

E

E

G

G

I

V

R

N

P

E

A

A

D

S

P

E

A

D

Y

F

L

L

R

S

G

K

L

L

R

Q

A

E

L

I

A

C

D

K

Q

E

F

K

G

D

L

V

L

L

L

I

M

I

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

I

I

G

G

R

R

T

T

G

G

K

E

L

F

F

Y

A

A

H

Y

E

Q

W

H

S

F

G

N

I

L

A

K

P

P

D

D

V

V

M

I

G

A

L

L

A

A

K
|
K

G

G

L

L

G

G

F

V

P

P

V

I

G

G

A

A

V

I

L

L

A

A

T

R

E

E

K

E

A

V

A

A

S

Q

C

S

M

F

T

T

P

P

G

G

T

S

H

H

G

G

C

S

T
|
T

F

F

G

G

N

N

P

P

L

L

A

A

M

C

A

R

V

A

A

G

E

T

A

V

V

V

L

V

D

D

E

E

V

V

M

-

A

-

P

E

G

K

F

L

L

L

E

P

R

H

V

V

Q

R

A

E

V

V

A

G

A

N

L

Y

L

F

R

K

G

E

R

K

L

L

D

K

D

E

L

L

A

G

R

K

R

-

Y

-

P

-

G

-

A

-

I

-

A

G

Q

K

V

V

R

K

G

G

Q

R

G

G

L

L

M

M

L

L

G

G

L

L

K

E

T

L

V

E

P

R

V

E

N

C

T

K

E

D

F

Y

N

V

A

L

K

K

L

A

F

L

A

E

A

K

G

G

L

L

A

I

V

N

G

C

A

T

G

A

D

G

N

K

V

V

V

L

R
|
R

L

F

V

L

P

P

L

L

I

I

G

Q

E

K

A

E

E

H

V

I

E

D

R

R

A

A

V

I

E

S

I

V

I

L

active site: F127 (= F130), E179 (= E182), D212 (= D215), Q215 (= Q218), K241 (= K244), T270 (= T273), R352 (= R363)
binding pyridoxal-5'-phosphate: G95 (= G97), T96 (≠ A98), F127 (= F130), H128 (= H131), E179 (= E182), D212 (= D215), V214 (= V217), K241 (= K244)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
40% identity, 97% coverage: 6:382/388 of query aligns to 4:372/376 of O66442

query
sites
O66442

L

L

L

M

P

N

V

N

F

Y

D

A

R

R

T

L

D

P

V

V

I

K

F

F

E

V

Q

R

G

G

E

K

G

G

A

V

W

Y

L

L

T

Y

A

D

S

E

D

E

G

G

R

K

R

E

F

Y

L

L

D

D

F

F

G

V

A

S

G

G

V

I

A

G

V

V

N

N

A

S

L

L

G

G

H

H

A

A

H

Y

P

P

R

K

L

L

V

T

A

E

A

A

L

L

E

K

A

E

Q

Q

A

V

G

E

K

K

L

L

W

L

H

H

C

V

S

S

N

N

L

L

Y

Y

R

E

V

N

P

P

G

W

Q

Q

E

E

R

E

V

L

A

A

E

H

R

K

L

L

I

V

K

K

A

H

S

F

F

W

A

T

D

E

-

G

T

K

A

V

F

F

C

A

N

N

T

S

G
|
G

A
x
T

E

E

A

S

M

V

E

E

L

A

V

A

I

I

K

K

I

L

A

A

R

R

R

K

Y

Y

H

W

H

R

C

D

A

K

G

G

R

K

P

-

E

N

R

K

N

W

R

K

I

F

V

I

A

S

C

F

T

E

G

N

S

S

F

F

H

H

G

G

R

R

T

T

I

Y

A

G

T

S

L

L

A

S

A

A

A

T

G

G

T

Q

P

P

K

K

Y

F

L

H

E

K

G

G

F

F

G

E

P

P

V

L

A

V

Q

P

G

G

F

F

D

S

H

Y

V

A

P

K

Y

L

G

N

D

D

L

I

E

D

A

S

A

V

R

Y

G

K

A

L

I

L

G

D

S

E

N

E

T

T

A

A

A

G

L

I

V

I

E

E

P

V

V

I

Q

Q

G

G

E

E

G

G

I

V

R

N

P

E

A

A

D

S

P

E

A

D

Y

F

L

L

R

S

G

K

L

L

R

Q

A

E

L

I

A

C

D

K

Q

E

F

K

G

D

L

V

L

L

L

I

M

I

D

D

E

E

V

V

Q

Q

T

T

G

G

I

I

G

G

R

R

T

T

G

G

K

E

L

F

F

Y

A

A

H

Y

E

Q

W

H

S

F

G

N

I

L

A

K

P

P

D

D

V

V

M

I

G

A

L

L

A

A

K
|
K

G

G

L

L

G

G

F

V

P

P

V

I

G

G

A

A

V

I

L

L

A

A

T

R

E

E

K

E

A

V

A

A

S

Q

C

S

M

F

T

T

P

P

G

G

T

S

H

H

G

G

C

S

T
|
T

F

F

G

G

N

N

P

P

L

L

A

A

M

C

A

R

V

A

A

G

E

T

A

V

V

V

L

V

D

D

E

E

V

V

M

-

A

-

P

E

G

K

F

L

L

L

E

P

R

H

V

V

Q

R

A

E

V

V

A

G

A

N

L

Y

L

F

R

K

G

E

R

K

L

L

D

K

D

E

L

L

A

G

R

K

R

-

Y

-

P

-

G

-

A

-

I

-

A

G

Q

K

V

V

R

K

G

G

Q

R

G

G

L

L

M

M

L

L

G

G

L

L

K

E

T

L

V

E

P

R

V

E

N

C

T

K

E

D

F

Y

N

V

A

L

K

K

L

A

F

L

A

E

A

K

G

G

L

L

A

I

V

N

G

C

A

T

G

A

D

G

N

K

V

V

V

L

R

R

L

F

V

L

P

P

L

L

I

I

G

Q

E

K

A

E

E

H

V

I

E

D

R

R

A

A

V

I

E

S

I

V

I

L

GT 96:97 (≠ GA 97:98) binding pyridoxal 5'-phosphate
K242 (= K244) modified: N6-(pyridoxal phosphate)lysine
T271 (= T273) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
40% identity, 98% coverage: 2:380/388 of query aligns to 8:389/402 of 4jevB

query
sites
4jevB

F

F

P

D

D

E

N

V

L

I

L

L

P

P

V

V

F

Y

D

A

R

P

T

A

D

D

V

F

I

I

F

P

E

V

Q

K

G

G

E

K

G

G

A

S

W

R

L

V

T

W

A

D

S

Q

D

Q

G

G

R

K

R

E

F

Y

L

I

D

D

F

F

G

A

A

G

G

G

V
x
I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

A

C

H

H

P

P

R

A

L

L

V

V

A

E

A

A

L

L

E

K

A

S

Q

Q

A

G

G

E

K

T

L

L

W

W

H

H

C

T

S

S

N

N

L

V

Y

F

R

T

V

N

P

E

G

P

Q

A

E

L

R

R

V

L

A

G

E

R

R

K

L

L

I

I

K

D

A

A

S

T

F

F

A

A

D

E

T

R

A

V

F

L

F

F

C

M

N

N

T
x
S

G
|
G

A
x
T

E

E

A

A

M

N

E

E

L

T

V

A

I

F

K

K

I

L

A

A

R

R

R

H

Y

Y

H

A

H

C

C

V

A

R

G

H

R

S

P

P

E

F

R

K

N

T

R

K

I

I

V

I

A

A

C

F

T

H

G

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

I

L

A

F

T

T

L

V

A

S

A

V

A

G

G

G

T

Q

P

P

K

K

Y

Y

L

S

E

D

G

G

F

F

G

G

P

P

V

K

A

P

Q

A

G

D

F

I

D

I

H

H

V

V

P

P

Y

F

G

N

D

D

L

L

E

H

A

A

A

V

R

K

G

A

A

V

I

M

G

D

S

D

N

H

T

T

A

C

A

A

L

V

V

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

I

V

R

Q

P

A

A

A

D

T

P

P

A

E

Y

F

L

L

R

K

G

G

L

L

R

R

A

D

L

L

A

C

D

D

Q

E

F

H

G

Q

L

A

L

L

L

V

M

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

H

Y

E

M

W

H

S

Y

G

G

I

V

A

T

P

P

D

D

V

I

M

L

G

T

L

S

A

A

K
|
K

G

A

L

L

G

G

F

F

P

P

V

V

G

S

A

A

V

M

L

L

A

T

T

T

E

Q

K

E

A

I

A

A

S

S

C

A

M

F

T

H

P

V

G

G

T

S

H

H

G

G

C

S

T
|
T

F

Y

G

G

N

N

P

P

L

L

A

A

M

C

A

A

V

V

A

A

E

G

A

A

V

T

L

F

D

D

E

I

V

I

M

N

A

T

P

P

G

E

F

V

L

L

E

Q

R

G

V

I

Q

H

A

T

V

K

A

R

A

Q

L

Q

L

F

R

V

G

Q

R

H

L

L

D

Q

D

A

L

I

A

D

R

E

R

Q

Y

F

P

-

G

D

A

I

I

F

A

S

Q

D

V

I

R

R

G

G

Q

M

G

G

L

L

M

L

L

I

G

G

L

A

K

E

T

L

V

K

P

P

V

-

N

K

T

Y

E

K

F

G

N

R

A

A

K

R

L

D

F

F

-

L

-

Y

-

A

-

G

-

A

A

E

A

A

G

G

L

V

L

M

A

V

V

L

G

N

A

A

G

G

D

A

N

D

V

V

V

M

R
|
R

L

F

V

A

P

P

P

S

L

L

I

V

G

E

E

E

A

A

E

D

V

I

E

H

R

E

A

G

V

M

E

Q

active site: F136 (= F130), E188 (= E182), D221 (= D215), Q224 (= Q218), K250 (= K244), T279 (= T273), R372 (= R363)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (≠ V40), S102 (≠ T96), G103 (= G97), T104 (≠ A98), F136 (= F130), H137 (= H131), E188 (= E182), E193 (= E187), D221 (= D215), V223 (= V217), Q224 (= Q218), K250 (= K244), R372 (= R363)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 98% coverage: 2:380/388 of query aligns to 13:394/405 of P40732

query
sites
P40732

F

F

P

D

D

E

N

V

L

I

L

L

P

P

V

V

F

Y

D

A

R

P

T

A

D

D

V

F

I

I

F

P

E

V

Q

K

G

G

E

K

G

G

A

S

W

R

L

V

T

W

A

D

S

Q

D

Q

G

G

R

K

R

E

F

Y

L

I

D

D

F

F

G

A

A

G

G

G

V

I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

A

C

H

H

P

P

R

A

L

L

V

V

A

E

A

A

L

L

E

K

A

S

Q

Q

A

G

G

E

K

T

L

L

W

W

H

H

C

T

S

S

N

N

L

V

Y

F

R

T

V

N

P

E

G

P

Q

A

E

L

R

R

V

L

A

G

E

R

R

K

L

L

I

I

K

D

A

A

S

T

F

F

A

A

D

E

T

R

A

V

F

L

F

F

C

M

N

N

T

S

G
|
G

A
x
T

E

E

A

A

M

N

E

E

L

T

V

A

I

F

K

K

I

L

A

A

R

R

R

H

Y

Y

H

A

H

C

C

V

A

R

G

H

R

S

P

P

E

F

R

K

N

T

R

K

I

I

V

I

A

A

C

F

T

H

G

N

S

A

F

F

H

H

G

G

R

R

T

S

I

L

A

F

T

T

L

V

A

S

A

V

A

G

G

G

T

Q

P

P

K

K

Y

Y

L

S

E

D

G

G

F

F

G

G

P

P

V

K

A

P

Q

A

G

D

F

I

D

I

H

H

V

V

P

P

Y

F

G

N

D

D

L

L

E

H

A

A

A

V

R

K

G

A

A

V

I

M

G

D

S

D

N

H

T

T

A

C

A

A

L

V

V

V

E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

I

V

R

Q

P

A

A

A

D

T

P

P

A

E

Y

F

L

L

R

K

G

G

L

L

R

R

A

D

L

L

A

C

D

D

Q

E

F

H

G

Q

L

A

L

L

L

V

M

F

D

D

E

E

V

V

Q

Q

T

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

H

Y

E

M

W

H

S

Y

G

G

I

V

A

T

P

P

D

D

V

I

M

L

G

T

L

S

A

A

K
|
K

G

A

L

L

G

G

F

F

P

P

V

V

G

S

A

A

V

M

L

L

A

T

T

T

E

Q

K

E

A

I

A

A

S

S

C

A

M

F

T

H

P

V

G

G

T

S

H

H

G

G

C

S

T
|
T

F

Y

G

G

N

N

P

P

L

L

A

A

M

C

A

A

V

V

A

A

E

G

A

A

V

A

L

F

D

D

E

I

V

I

M

N

A

T

P

P

G

E

F

V

L

L

E

Q

R

G

V

I

Q

H

A

T

V

K

A

R

A

Q

L

Q

L

F

R

V

G

Q

R

H

L

L

D

Q

D

A

L

I

A

D

R

E

R

Q

Y

F

P

-

G

D

A

I

I

F

A

S

Q

D

V

I

R

R

G

G

Q

M

G

G

L

L

M

L

L

I

G

G

L

A

K

E

T

L

V

K

P

P

V

-

N

K

T

Y

E

K

F

G

N

R

A

A

K

R

L

D

F

F

-

L

-

Y

-

A

-

G

-

A

A

E

A

A

G

G

L

V

L

M

A

V

V

L

G

N

A

A

G

G

D

A

N

D

V

V

V

M

R

R

L

F

V

A

P

P

P

S

L

L

I

V

G

E

E

E

A

A

E

D

V

I

E

H

R

E

A

G

V

M

E

Q

GT 108:109 (≠ GA 97:98) binding pyridoxal 5'-phosphate
K255 (= K244) modified: N6-(pyridoxal phosphate)lysine
T284 (= T273) binding pyridoxal 5'-phosphate

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 96% coverage: 10:383/388 of query aligns to 73:450/457 of Q9M8M7

query
sites
Q9M8M7

F

Y

D

A

R

R

T

A

D

P

V

V

I

V

F

L

E

S

Q

S

G

G

E

K

G

G

A

C

W

K

L

L

T

F

A

D

S

P

D

E

G

G

R

K

R

E

F

Y

L

L

D

D

F

C

G

A

A

S

G

G

V

I

A

A

V

V

N

N

A

A

L

L

G

G

H

H

A

G

H

D

P

P

R

D

L

W

V

L

A

R

A

A

L

V

E

T

A

E

Q

Q

A

A

G

G

K

V

L

L

W

A

H

H

C

V

S

S

N

N

L

V

Y

Y

R

Y

V

T

P

I

G

P

Q

Q

E

I

R

E

V

L

A

A

E

K

R

R

L

L

I

V

K

A

A

S

S

S

F

F

A

A

D

D

T

R

A

V

F

F

C

C

N

N

T

S

G

G

A

T

E

E

A

A

M

N

E

E

L

A

V

A

I

I

K

K

I

F

A

S

R

R

R

K

Y

F

H

Q

H

R

C

F

A

T

G

-

R

H

P

P

E

E

R

D

N

K

R

E

I

V

-

A

-

T

-

G

-

F

V

I

A

A

C

F

T

T

G

N

S

S

F

F

H

H

G

G

R

R

T

T

I

L

A

G

T

A

L

L

A

A

A

L

A

T

G

S

T

K

P

E

K

Q

Y

Y

L

R

E

T

G

P

F

F

G

E

P

P

V

I

A

M

Q

P

G

G

F

V

D

T

H

F

V

L

P

E

Y

Y

G

G

D

N

L

I

E

Q

A

A

R

T

G

D

A

L

I

I

G

R

S

S

-

G

N

K

T

I

A

A

L

V

L

F

V

V

E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

I

I

R

Y

P

S

A

A

D

T

P

K

A

E

Y

F

L

L

R

Q

G

S

L

L

R

R

A

S

L

A

A

C

D

D

Q

A

F

A

G

G

L

S

L

L

L

V

M

F

D

D

E

E

V

V

Q

Q

T

C

G

G

I

L

G

G

R

R

T

T

G

G

K

L

L

M

F

W

A

A

H

Y

E

E

W

A

S

F

G

G

I

V

A

T

P

P

D

D

V

I

M

M

G

T

L

V

A

A

K

K

G

P

L

L

G

A

G

G

F

L

P

P

V

I

G

G

A

A

V

V

L

L

A

V

T

T

E

E

K

K

A

V

A

A

S

E

C

T

M

I

T

N

P

Y

G

G

T

D

H

H

G

G

C

S

T

T

F

F

G

A

G

G

N

S

P

P

L

L

A

V

M

C

A

S

V

A

A

A

E

I

A

A

V

V

L

M

D

D

E

K

V

V

M

S

A

K

P

P

G

S

F

F

L

L

E

S

R

S

V

V

Q

S

A

N

V

K

A

G

A

R

L

Y

L

F

R

R

G

-

R

-

L

-

D

D

D

L

L

L

A

V

R

K

Y

L

P

G

G

G

A

N

-

S

-

H

I

V

A

K

Q

E

V

V

R

R

G

G

Q

E

G

G

L

L

M

I

L

I

G

G

L

V

K

E

-

L

T

D

V

V

P

P

V

A

N

S

T

S

E

L

F

V

N

D

A

A

K

-

L

C

F

R

A

D

A

S

G

G

L

L

A

I

V

L

G

T

A

A

G

G

D

K

-

G

N

N

V

V

R

R

L

I

V

V

P

P

L

L

I

V

I

I

G

S

E

E

A

E

E

E

V

I

E

E

R

R

A

A

V

V

E

E

I

I

I

M

D

S

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
39% identity, 98% coverage: 2:380/388 of query aligns to 8:384/397 of 4jewA

query
sites
4jewA

F

F

P

D

D

E

N

V

L

I

L

L

P

P

V

V

F

Y

D

A

R

P

T

A

D

D

V

F

I

I

F

P

E

V

Q
x
K

G

G

E
x
K

G

G

A

S

W

R

L

V

T
x
W

A

D

S

Q

D

Q

G

G

R

K

R

E

F

Y

L

I

D

D

F

F

G

A

A

G

G

G

V

I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

A

C

H

H

P

P

R

A

L

L

V

V

A

E

A

A

L

L

E

K

A

S

Q

Q

A

G

G

E

K

T

L

L

W

W

H

H

C

T

S

S

N

N

L

V

Y

F

R

T

V

N

P

E

G

P

Q

A

E

L

R

R

V

L

A

G

E

R

R

K

L

L

I

I

K

D

A

A

S

T

F

F

A

A

D

E

T

R

A

V

F

L

F

F

C

M

N

N

T

S

G
|
G

A
x
T

E

E

A

A

M

N

E

E

L

T

V

A

I

F

K

K

I

L

A

A

R

R

R

H

Y

Y

H

A

H

C

C

V

A

R

G

H

R

S

P

P

E

F

R

K

N

T

R

K

I

I

V

I

A

A

C

F

T

H

G

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

S

I

L

A

F

T

T

L

V

A

S

A

V

A

G

G

G

T

Q

P

P

K

K

Y

Y

L

S

E

D

G

G

F

F

G

G

P

P

V

K

A

P

Q

A

G

D

F

I

D

I

H

H

V

V

P

P

Y

F

G

N

D

D

L

L

E

H

A

A

A

V

R

K

G

A

A

V

I

M

G

D

S

D

N

H

T

T

A

C

A

A

L

V

V

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

I

V

R

Q

P

A

A

A

D

T

P

P

A

E

Y

F

L

L

R

K

G

G

L

L

R

R

A

D

L

L

A

C

D

D

Q

E

F

H

G

Q

L

A

L

L

L

V

M

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

H

Y

E

M

W

H

S

Y

G

G

I

V

A

T

P

P

D

D

V

I

M

L

G

T

L

S

A

A

K
|
K

G

A

L

L

G

G

F

F

P

P

V

V

G

S

A

A

V

M

L

L

A

T

T

T

E

Q

K

E

A

I

A

A

S

S

C

A

M

F

T

-

P

-

G

-

T

-

H

-

G

G

C

S

T
|
T

F

Y

G

G

N

N

P

P

L

L

A

A

M

C

A

A

V

V

A

A

E

G

A

A

V

T

L

F

D

D

E

I

V

I

M

N

A

T

P

P

G

E

F

V

L

L

E

Q

R

G

V

I

Q

H

A

T

V

K

A

R

A

Q

L

Q

L

F

R

V

G

Q

R

H

L

L

D

Q

D

A

L

I

A

D

R

E

R

Q

Y

F

P

-

G

D

A

I

I

F

A

S

Q

D

V

I

R

R

G

G

Q

M

G

G

L

L

M

L

L

I

G

G

L

A

K

E

T

L

V

K

P

P

V

-

N

K

T

Y

E

K

F

G

N

R

A

A

K

R

L

D

F

F

-

L

-

Y

-

A

-

G

-

A

A

E

A

A

G

G

L

V

L

M

A

V

V

L

G

N

A

A

G

G

D

A

N

D

V

V

V

M

R
|
R

L

F

V

A

P

P

P

S

L

L

I

V

G

E

E

E

A

A

E

D

V

I

E

H

R

E

A

G

V

M

E

Q

active site: F136 (= F130), E188 (= E182), D221 (= D215), Q224 (= Q218), K250 (= K244), T274 (= T273), R367 (= R363)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: G103 (= G97), T104 (≠ A98), F136 (= F130), H137 (= H131), R139 (= R133), E188 (= E182), E193 (= E187), D221 (= D215), V223 (= V217), K250 (= K244)
binding picric acid: K25 (≠ Q19), K27 (≠ E21), W32 (≠ T26)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
39% identity, 98% coverage: 2:380/388 of query aligns to 2:378/389 of 2pb0A

query
sites
2pb0A

F

F

P

D

D

E

N

V

L

I

L

L

P

P

V

V

F

Y

D

A

R

P

T

A

D

D

V

F

I

I

F

P

E

V

Q

K

G

G

E

K

G

G

A

S

W

R

L

V

T

W

A

D

S

Q

D

Q

G

G

R

K

R

E

F

Y

L

I

D

D

F

F

G

A

A

G

G

G

V

I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

A

C

H

H

P

P

R

A

L

L

V

V

A

E

A

A

L

L

E

K

A

S

Q

Q

A

G

G

E

K

T

L

L

W

W

H

H

C

T

S

S

N

N

L

V

Y

F

R

T

V

N

P

E

G

P

Q

A

E

L

R

R

V

L

A

G

E

R

R

K

L

L

I

I

K

D

A

A

S

T

F

F

A

A

D

E

T

R

A

V

F

L

F

F

C

M

N

N

T
x
S

G
|
G

A
x
T

E

E

A

A

M

N

E

E

L

T

V

A

I

F

K

K

I

L

A

A

R

R

R

H

Y

Y

H

A

H

C

C

V

A

R

G

H

R

S

P

P

E

F

R

K

N

T

R

K

I

I

V

I

A

A

C

F

T

H

G

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

I

L

A

F

T

T

L

V

A

S

A

V

A

G

G

G

T

Q

P

P

K

K

Y

Y

L

S

E

D

G

G

F

F

G

G

P

P

V

K

A

P

Q

A

G

D

F

I

D

I

H

H

V

V

P

P

Y

F

G

N

D

D

L

L

E

H

A

A

A

V

R

K

G

A

A

V

I

M

G

D

S

D

N

H

T

T

A

C

A

A

L

V

V

V

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

I

V

R

Q

P

A

A

A

D

T

P

P

A

E

Y

F

L

L

R

K

G

G

L

L

R

R

A

D

L

L

A

C

D

D

Q

E

F

H

G

Q

L

A

L

L

L

V

M

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

H

Y

E

M

W

H

S

Y

G

G

I

V

A

T

P

P

D

D

V

I

M

L

G

T

L

S

A

A

K
|
K

G

A

L

L

G

G

F

F

P

P

V

V

G

S

A

A

V

M

L

L

A

T

T

T

E

Q

K

E

A

I

A

A

S

S

C

A

M

F

T

-

P

-

G

-

T

-

H

-

G

G

C

S

T
|
T

F

Y

G

G

N

N

P

P

L

L

A

A

M

C

A

A

V

V

A

A

E

G

A

A

V

A

L

F