SitesBLAST

Q7WTU0 Arsenate respiratory reductase molybdopterin-containing subunit ArrA; Arsenate respiratory reductase large subunit; ARR large subunit; EC 1.20.99.1 from Shewanella sp. (strain ANA-3) (see paper)
25% identity, 44% coverage: 14:418/922 of query aligns to 55:397/854 of Q7WTU0

query
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C61 (= C20) binding
C64 (= C23) binding
C68 (≠ P27) binding
C96 (= C59) binding
R165 (≠ G133) binding
Y166 (≠ A134) binding
H189 (≠ F156) binding
S190 (≠ G157) binding
C193 (≠ S160) binding
K198 (= K165) binding
Y210 (≠ H178) binding

A0A369NIV7 Dopamine dehydroxylase; EC 1.1.-.- from Eggerthella lenta (Eubacterium lentum) (see paper)
25% identity, 30% coverage: 10:285/922 of query aligns to 43:300/1017 of A0A369NIV7

query
sites
A0A369NIV7

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Sites not aligning to the query:

506 Crucial for dopamine dehydroxylation activity

P18775 Dimethyl sulfoxide reductase DmsA; DMSO reductase; DMSOR; Me2SO reductase; EC 1.8.5.3 from Escherichia coli (strain K12) (see 4 papers)
21% identity, 66% coverage: 30:638/922 of query aligns to 73:583/814 of P18775

query
sites
P18775

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Q

R

G

F

E

E

A

C

R

K

D

T

F

I

T

Y

D

E

I

M

A

T

T

S

D

E

L

L

A

A

V

K

R

R

T

L

G

G

L

V

Q

E

E

Q

K

Q

Y

F

C104 (= C59) mutation to S: No alteration of the growth, expression, or catalytic activities.
R106 (≠ K61) mutation to S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry.

Sites not aligning to the query:

1:45 signal peptide, Tat-type signal
17 R→S: Not targeted to the membrane, does not support anaerobic growth.
57 K→D: No alteration of the growth, expression, or catalytic activities.
67 C→S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry.
71 C→S: Cannot support growth.

2ivfA Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
26% identity, 35% coverage: 59:378/922 of query aligns to 63:367/912 of 2ivfA

query
sites
2ivfA

C
|
C

V

Q

K
|
K

A
x
G

F

S

G

A

L

F

I

N

Q

N

K

N

S

L

Y

Y

T

G

P

D

H

E

R

R

I

V

L

K

T

Y

P

P

M

L

K

K

R

R

T

V

N

-

P

-

K

-

G

G

K

K

D

R

Q

G

D

E

P

G

G

K

F

W

V

K

P

R

I

V

S

S

W

W

D

D

E

E

A

A

M

A

G

G

T

D

I

I

A

A

E

D

R

S

L

I

-

I

N

D

A

S

I

F

R

E

A

A

E

Q

G

G

-

S

-

D

-

G

-

F

L

I

L

L

D

D

A

A

Q
x
P

G
x
H

Y

V

P
x
H

K
x
A

F

G

A

S

A

I

S

A

F

W

G

G

G

A

A

G

G

F

T

R

P

M

T

T

Q

Y

Y

L

M

M

G

D

S

G

L

V

T

S

A

P

F

D

L

I

S

N

A

-

W

-

G

-

P

-

I

V

D
|
D

F
x
I

G

G

F

-

G
x
D

S

T

G

Y

Q

M

G

G

V

-

K

-

C

-

Y

-

H

-

S

A

E

F

H

H

L

T

Y

F

G

G

E

K

F

M

W

-

H

H

R

M

G

G

Y

Y

V

-

I

-

T

S

P

A

D

D

T

N

P

L

R

L

A

D

N

A

Y

E

I

L

I

I

S

F

C

M

G

T

T
x
C

N
x
S

T
x
N

E

W

A

S

S
x
Y

S
x
T

G

Y

V

P

C

S

G

S

V

Y

Y

H

R

F

H

L

A

S

E

E

A

A

R

R

A

Y

R

K

G

G

A

A

I

E

R

V

V

V

Q

V

V
x
I

E

A

P
|
P

H
x
D

L

F

S
x
N

V

P

T

T

G

T

A

P

C

A

S

A

A

D

E

L

W

H

L

V

P

P

I

V

K

R

P

V

K
x
G

T

S

D
|
D

A

A

F

F

L

W

M

L

T

G

L

L

L

S

N

Q

V

V

L

M

V

I

F

D

E

E

A

-

P

-

R

-

E

K

R

L

L

F

D

D

L

R

D

Q

F

F

L

V

R

C

N

E

T

Q

T

T

S

D

S

L

P

P

Y

L

L

L

V

V

G

R

P

M

D

D

-

T

G

G

W

K

Y

F

L

L

R

S

D

A

P

E

-

D

-

V

-

D

-

G

E

E

T

A

H

K

K

Q

P

F

L

Y

L

F

W

F

D

D

E

E

K

K

T

A

G

G

R

-

A

S

V

V

P

R

H

K

D

A

T

S

P

R

G

G

A

T

V

L

-

K

-

L

-

D

-

F

-

M

P

P

A

A

L

L

E

E

G

G

R

T

F

F

T

S

V

A

P

R

K

L

A

K

V

-

T

-

L

-

G

-

A

-

D

-

E

-

D

-

R

-

W

-

E

N

H

G

T

K

D

T

V

I

E

Q

G

V

V

R

T

T

A

V

F

F

T

E

K

G

F

L

I

R

E

E

H

H

V

L

K

K

G

D

Y

Y

T

T

P

P

A

E

W

K

G

A

A

S

K

A

V

K

C

C

D

G

V

V

P

P

E

V

A

S

R

L

I

I

R

R

K

E

I

L

active site: K65 (= K61), P127 (≠ Q122), D156 (= D155), I157 (≠ F156)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: H128 (≠ G123), H130 (≠ P125), A131 (≠ K126), I157 (≠ F156), D159 (≠ G159)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D159 (≠ G159), C190 (≠ T198), S191 (≠ N199), N192 (≠ T200), Y195 (≠ S203), T196 (≠ S204), I217 (≠ V225), P219 (= P227), D220 (≠ H228), N222 (≠ S230), G237 (≠ K245), D239 (= D247)
binding iron/sulfur cluster: C63 (= C59), G66 (≠ A62)

Sites not aligning to the query:

active site: 430
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: 386, 543, 544, 545, 548, 549, 551, 571, 572, 573, 574, 588, 590, 591, 594, 623, 785, 791, 792, 856, 868, 869, 903
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 21, 23, 381, 382, 383, 783, 784, 785, 786, 789, 790, 791
binding iron/sulfur cluster: 18, 22, 26, 28, 30, 62

2vpwA Polysulfide reductase with bound menaquinone (see paper)
28% identity, 23% coverage: 54:261/922 of query aligns to 45:242/734 of 2vpwA

query
sites
2vpwA

A

S

E

R

G

G

K

R

V
x
L

C
|
C

V

P

K
x
R

A
x
G

F

Q

G

G

L

A

I

P

Q

Q

K

T

S

T

Y

Y

T

D

P

P

H

D

R

R

I

L

L

K

T

R

P

P

M

L

K

I

R

R

T

V

N

-

P

-

K

-

K

E

G

G

K

S

D

Q

Q

R

D

G

P

E

G

G

-

K

F

Y

V

R

P

V

I

A

S

T

W

W

D

E

E

E

A

A

M

L

G

D

T

H

I

I

A

A

E

K

R

K

L

M

N

L

A

E

I

I

R

R

A

-

E

-

G

-

L

-

D

-

A

-

Q

E

G

K

Y

Y

P

G

K

P

F

E

A

A

A

I

S

A

F

F

G

F

G

G

A
x
H

G

G

T

T

P

G

T
x
D

Q

Y

Y

W

M

-

G

-

S

-

L

F

T

V

A

D

F

F

L

L

-

P

S

A

A

A

W

W

G

G

P

S

I

P

D

N

F

-

G

-

F

-

G

A

S

A

G
x
K

Q

P

G

S

V

V

K

S

C
x
L

Y
x
C

H
x
T

S

A

E

P

H

R

L

E

Y

V

G

A

E

S

F

Q

W

W

H

V

R

F

G

G

Y

R

V

P

I

I

T

G

P

G

D

H

T

E

P

P

-

I

-

D

-

W

-

E

R

N

A

A

N

R

Y

Y

I

I

I

V

S

L

C

I

G
|
G

T
x
H

N
x
H

T

I

E

G

A

E

S
x
D

S
x
T

G
x
H

V
x
N

C

T

G

Q

V

L

Y

Q

R

D

H

F

A

A

E

L

A

A

R

L

A

K

R

N

G

G

A

A

I

K

R

V

V

V

Q

V

V
|
V

E
x
D

P
|
P

H
x
R

L

F

S

S

V

T

T

A

G

A

A

A

C

K

S

A

A

H

E

R

W

W

L

L

P

P

I

I

K

K

P

P

K

G

T

T

D
|
D

A

T

A

A

F

L

L

L

M

L

T

A

L

W

L

I

N

H

V

V

L

L

V

I

F

Y

E

E

active site: R52 (≠ K61), H116 (≠ A134), D120 (≠ T138), K138 (≠ G161), C144 (≠ Y167), T145 (≠ H168)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: R52 (≠ K61), H116 (≠ A134), L143 (≠ C166), C144 (≠ Y167), G178 (= G197), H179 (≠ T198), H180 (≠ N199), D184 (≠ S203), T185 (≠ S204), H186 (≠ G205), V206 (= V225), D207 (≠ E226), P208 (= P227), R209 (≠ H228), D228 (= D247)
binding iron/sulfur cluster: L49 (≠ V58), C50 (= C59), R52 (≠ K61), G53 (≠ A62), N187 (≠ V206)

Sites not aligning to the query:

active site: 303, 335, 340, 341, 351
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 302, 303, 304, 307, 308, 410, 411, 412, 415, 416, 436, 437, 441, 453, 459, 593, 594, 596, 596, 600, 601, 602, 602, 603, 604, 668, 687, 688, 706
binding iron/sulfur cluster: 15, 17, 18, 20, 22

7l5sA Crystal structure of haemophilus influenzae mtsz at ph 5.5 (see paper)
21% identity, 56% coverage: 347:859/922 of query aligns to 267:700/771 of 7l5sA

query
sites
7l5sA

T

S

A

G

F

Y

T

A

K

K

F

F

I

E

E

E

H

Y

V

L

K

L

G

G

Y

K

T

T

P

D

A

G

-

Q

-

P

-

K

-

T

-

A

-

E

W

W

G

A

A

A

K

K

V

I

C

C

D

G

V

V

P

P

E

A

A

E

R

T

I

I

R

K

K

Q

I

L

A

A

N

A

D

D

F

F

L

A

D

S

H

K

A

R

C

T

V

M

G

-

Q

-

T

-

I

-

E

-

I

-

D

-

G

-

Q

-

V

-

M

-

P

-

Y

-

R

-

P

-

V

-

A

-

V

-

L

-

G

-

K

-

T

-

V

L

N

M

N

G

G

G

W
|
W

G
|
G

-
x
M

-

Q

-
x
R

-
x
Q

-

R

-

H

G

G

A

E

E

Q

C

T

C

H

W

W

A

M

R

L

T

V

M

T

L

L

A

A

V

S

L

M

V

L

G

G

A

Q

L

I

E

G

V

L

P

P

G

G

T

G

V

F

G

G

T

L

T

S

I
x
Y

R
x
H

I
x
Y

N

S

R

N

P

G

A

G

A

-

N

-

R

-

L

V

E

P

S

T

F

A

E

T

G

G

C

G

L

I

D

I

G

G

F

S

M

I

E

T

Y

A

P

-

F

-

N

S

P

P

T

S

D

L

K

D

D

D

T

T

W

S

K

K

-

S

A

A

N

F

P

P

I

L

I

A

R

R

N

I

A

A

Y

D

K

V

M

L

L

L

V

H

P

P

L

G

V

-

G

-

N

-

S

-

W

-

S

-

P

-

A

-

L

-

G

-

P

-

T

-

Q

-

F

-

S

-

Y

-

M

-

F

-

L

-

D

K

E

K

P

I

Q

Q

D

Y

Q

N

I

G

P

T

R

E

A

I

T

T

F

Y

P

P

E

D

F

I

L

K

L

A

V

V

Y

Y

R

W

T

A

-
x
G

N
|
N

P

P

V

F

I

V

S
x
H

F

H

W
x
Q

D

D

T

T

D

N

R

T

V

L

A

V

S

K

V

A

V

F

S

Q

R

K

M

P

P

D

F

V

V

V

V

I

C

V

F
x
N

A
x
E

H
x
V

T

N

R

W

D

T

E

P

T

T

N

A

H

R

F

M

A

A

D

D

I

I

L

V

L

L

P

P

D
x
A

A

T

T

T

D

S

L

Y

E

E

G
x
R

M

N

Q

D

L

L

I

T

R

M

I

A

G

G

G

D

T

Y

K

S

F

M

Q

M

E

S

Q

V

F

Y

W

P

K

M

Q

K

Q

Q

G

-

F

-

A

-

L

-

R

-

Q

-

P

-

S

V

V

V

K

P

P

P

Q

Q

G

F

E

E

A

A

R

K

D

N

F
x
D

T

Y

D

D

I

I

A

F

T

V

D

E

L

L

A

A

V

K

R

R

T

A

G

G

L

V

Q

E

E

E

K

Q

Y

Y

V

T

A

E

A

G

I

K

N

T

R

E

G

-

S

-

H

-

G

-

V

-

P

-

L

-

K

-

G

-

P

M

N

E

W

W

D

-

F

-

S

-

L

-

P

-

V

-

D

-

K

-

A

-

P

-

S

-

L

L

E

E

V

F

W

Y

D

N

A

A

S

A

C

F

R

S

S

A

A

A

S

R

A

A

E

N

L

R

S

V

D

-

G

-

A

A

E

M

S

P

Q

R

G

F

L

D

D

K

W

F

W

W

K

A

E

E

N

N

G

K

F

-

R

-

T

-

I

-

P

P

F

L

S

S

E

-

T

-

N

-

W

-

F

F

L

E

T

A

P

G

A

E

L

A

K

A

A

K

K

K

G

W

L

V

R

R

Y

Y

E

G

L

E

P

F

Y

R

Q

E

E

D

R

P

L

L

T

L

R

N

I

P

G

L

R

G

Q

T

L

P

A

S

N

G

R

K

L

I

K

E

E

-

A

-

G

-

I

-

T

-

W

-

D

-

H

-

Q

I

L

F

T

S

E

D

Y

V

R

V

P

E

L

K

M

M

E

N

W

Y

H

N

D

D

F

C

P

K

G

G

Y

H

-

P

-

S

W

W

E

M

Q

E

S

P

V

E

E

F

H

A

G

G

G

N

K

V

V

T

A

E

D

E

F

Y

P

P

F

L

W

A

V

L

V

V

T
|
T

A
x
P

R
x
H

S
x
P

M

Y

Q

Y

Y
x
R

A
x
L

W
x
H

G
x
S

S
x
Q

N

L

M

A

H

H

I

T

P

S

L

L

M

R

R

Q

E

K

V

Y

S

A

G

-

H

-

I

V

K

N

G

D

H

R

D

E

G

P

V

V

V

M

M

I

N

H

P

P

E

E

A

D

A

A

R

A

K

A

V

R

G

G

V

I

K

K

E

D

G

G

E

D

R

I

I

V

V

R

V

I

T

H

A

S

P

K

T

R

G

G

K

Q

S

V

V

L

A

A

G

G

R

A

V

A

V

V

L

T

S

E

Q

N

G

I

I

I

R

K

active site: W319 (= W416), Y355 (≠ I446), H356 (≠ R447), Y357 (≠ I448)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: W319 (= W416), G320 (= G417), M321 (vs. gap), R323 (vs. gap), R323 (vs. gap), Q324 (vs. gap), H356 (≠ R447), G422 (vs. gap), G423 (vs. gap), N424 (= N537), H428 (≠ S541), Q430 (≠ W543), N448 (≠ F561), E449 (≠ A562), V450 (≠ H563), A465 (≠ D578), R471 (≠ G584), D501 (≠ F621), T633 (= T790), P634 (≠ A791), H635 (≠ R792), H635 (≠ R792), P636 (≠ S793), R639 (≠ Y796), L640 (≠ A797), H641 (≠ W798), H641 (≠ W798), S642 (≠ G799), Q643 (≠ S800)

Sites not aligning to the query:

active site: 38, 113, 142, 150, 151
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 111, 112, 113, 113, 114, 115, 122, 143, 144, 144, 182, 187, 188, 191, 217, 218, 219, 220, 240, 707, 728, 745

7vw6A Cryo-em structure of formate dehydrogenase 1 from methylorubrum extorquens am1 (see paper)
26% identity, 26% coverage: 55:291/922 of query aligns to 257:509/913 of 7vw6A

query
sites
7vw6A

E

Q

G

N

K

R

V

L

C
|
C

V

V

K
|
K

A
x
G

F

R

G

F

L

G

I

F

Q

D

K

Y

S

V

Y

H

T

H

P

P

H

H

R

R

I

L

L

T

T

V

P

P

M

L

K

I

R

R

T

L

N

E

-

N

-

V

P

P

K

K

K

D

G

A

K

N

D

D

Q

Q

-

V

D

D

P

P

G

A

-

N

-

P

-

W

-

T

-

H

F

F

V

R

P

E

I

A

S

T

W

W

D

E

E

E

A

A

M

L

G

D

T

R

I

A

A

A

E

G

R

G

L

L

N

K

A

A

I

I

R

R

A

-

E

-

G

-

L

-

D

D

A

T

Q

N

G

G

Y

R

P

-

K

K

F

A

A

L

A

A

S

G

F

F

G

G

G

S

A

A

G

-

T

-

P

-

T

-

Q

-

Y

-

M
x
K

G

G

S

S

L

N

T

E

A

E

F

A

L

Y

S

L

A

F

W

Q

G

K

P

L

I

V

D

R

F

L

G

G

F

F

G

G

S

T

G

N

Q

N

G

V

V

D

K

H

C
|
C

Y

T

H

R

S

L

E
x
C

H

H

L

A

Y

S

G

S

-

V

-

A

-

L

-

M

E

E

F

G

W

L

H

N

R

S

G

G

Y

A

V

V

I

T

T

A

P

P

D

F

T

S

P

A

R

A

-

L

-

D

A

A

N

E

Y

V

I

I

I

V

S

V

C

I

G
|
G

T

A

N
|
N

T

P

E

T

A

V

S
x
N

S

H

G

P

V
|
V

C

A

G

A

V

T

Y

F

R

L

H

K

A

N

E

A

A

V

R

K

A

Q

R

R

G

G

A

A

I

K

R

L

V

I

Q

I

V

M

E
x
D

P
|
P

H
x
R

L

R

S

Q

V

T

T

L

G

S

A

R

C

H

S

A

A

Y

E

R

W

H

L

L

P

A

I
x
F

K

R

P

P

K
x
G

T

S

D
|
D

A

V

A

A

F

M

L

L

M

N

T

A

L

M

L

L

N

N

V

V

L

I

V

V

-

T

-

E

-

G

-

L

-

Y

-

D

-

E

-

Q

-

Y

-

I

-

A

-

G

-

Y

-

T

-

E

-

N

F

F

E

E

A

A

P

L

R

R

E

E

R

K

L

I

D

-

L

V

D

D

F

F

L

T

R

P

N

E

T

K

T

M

S

A

S

S

P

-

Y

-

L

V

V

C

G

G

P

I

D

D

G

A

W

E

Y

T

L

L

R

R

D

E

binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K263 (= K61), K339 (≠ M141), C364 (= C166), C368 (≠ E170), G402 (= G197), N404 (= N199), N408 (≠ S203), D431 (≠ E226), P432 (= P227), R433 (≠ H228), F447 (≠ I242), G450 (≠ K245), D452 (= D247)
binding iron/sulfur cluster: C261 (= C59), K263 (= K61), G264 (≠ A62), V411 (= V206)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 32, 34, 35, 45, 47, 48, 50, 64
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 525, 526, 527, 530, 531, 563, 564, 630, 632, 636, 656, 657, 658, 805, 807, 807, 808, 809, 811, 812, 813, 813, 814, 817, 879, 887, 903, 904
binding iron/sulfur cluster: 145, 146, 147, 148, 149, 151, 155, 161, 163, 164, 175, 188, 189, 190, 191, 192, 194, 198, 199, 200, 202, 203, 227, 230, 234

2e7zA Acetylene hydratase from pelobacter acetylenicus (see paper)
23% identity, 25% coverage: 49:274/922 of query aligns to 31:242/727 of 2e7zA

query
sites
2e7zA

A

S

D

E

T

P

H

H

P

P

A

T

E

T

-

P

G

N

K

S

V

I

C
|
C

V

M

K
|
K

A
x
S

F

V

G

N

L

A

I

D

Q

T

K

I

S

R

Y

T

T

H

P

K

H

D

R

R

I

V

L

L

T

Y

P

P

M

L

K

K

R

N

T

V

N

G

P

S

K

K

K

R

G

G

K

E

D

Q

Q

R

D

-

P

-

G

-

F

W

V

E

P

R

I

I

S

S

W

W

D

D

E

Q

A

A

M

L

G

D

T

E

I

I

A

A

E

E

R

K

L

L

N

K

A

K

I

I

R

I

A

A

E

K

G

-

L

-

D

-

A

-

Q

Y

G

G

Y

P

P

E

K

S

F

L

A

G

A

V

S

S

F

Q

G
x
T

G

E

A
x
I

G
x
N

T

Q

P

Q

T

S

Q

E

Y

Y

M

-

G

G

S

T

L

L

T

R

A

R

F

F

L

M

S

N

A

L

W

L

G

G

P

S

-

P

-

N

-
x
W

-

T

-

S

-
x
A

I

M

D

Y

F
x
M

G
x
C

F
x
I

G

G

S

N

G

T

Q

A

G

G

V

V

-

H

K

R

C

V

Y

T

H

H

S

G

E

S

H

Y

L

S

Y

F

G

A

E

S

F

F

W

-

H

-

R

-

G

-

Y

-

V

-

I

-

T

-

P

-

D

-

T

-

P

A

R

D

A

S

N

N

Y

C

I

L

S

F

C

I

G
|
G

T
x
K

N
|
N

T

L

E

S

A

N

S

H

S
x
N

G
x
W

V
|
V

C

S

G

Q

V

F

Y

N

R

D

H

L

A

K

E

A

A

A

R

L

A

K

R

R

G

G

A

C

I

K

R

L

V

I

Q

V

V
x
L

E
x
D

P
|
P

H
x
R

L

R

S

T

V

K

T

V

G

A

A

E

C

M

S

A

A

D

E

I

W

W

L

L

P

P

I

L

K

R

P
x
Y

K
x
G

T

T

D
|
D

A

A

F

L

L

F

M

L

T

G

L

M

L

I

N

N

V

V

L

I

V

I

F

N

E

E

A

-

P

-

R

-

E

Q

R

L

L

Y

D

D

L

K

D

E

F

F

L

V

R

E

N

N

active site: K45 (= K61), T108 (≠ G132), I110 (≠ A134), W131 (vs. gap), C138 (≠ G157), I139 (≠ F158)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K45 (= K61), T108 (≠ G132), N111 (≠ G135), A134 (vs. gap), M137 (≠ F156), C138 (≠ G157), G168 (= G197), K169 (≠ T198), N170 (= N199), N175 (≠ S204), W176 (≠ G205), L196 (≠ V225), D197 (≠ E226), P198 (= P227), R199 (≠ H228), Y215 (≠ P244), G216 (≠ K245), D218 (= D247)
binding iron/sulfur cluster: C43 (= C59), K45 (= K61), S46 (≠ A62), W176 (≠ G205), V177 (= V206)

Sites not aligning to the query:

active site: 292, 335, 336, 337
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 10, 292, 293, 296, 297, 413, 415, 419, 420, 439, 440, 441, 457, 462, 599, 602, 603, 603, 604, 605, 607, 608, 609, 609, 610, 611, 673, 696, 716, 717
binding iron/sulfur cluster: 6, 8, 9, 11, 12, 13

Q71EW5 Acetylene hydratase; Ahy; EC 4.2.1.112 from Syntrophotalea acetylenica (Pelobacter acetylenicus) (see 3 papers)
23% identity, 25% coverage: 49:274/922 of query aligns to 34:245/730 of Q71EW5

query
sites
Q71EW5

A

S

D

E

T

P

H

H

P

P

A

T

E

T

-

P

G

N

K

S

V

I

C
|
C

V

M

K
|
K

A

S

F

V

G

N

L

A

I

D

Q

T

K

I

S

R

Y

T

T

H

P

K

H

D

R

R

I

V

L

L

T

Y

P

P

M

L

K

K

R

N

T

V

N

G

P

S

K

K

K

R

G

G

K

E

D

Q

Q

R

D

-

P

-

G

-

F

W

V

E

P

R

I

I

S

S

W

W

D

D

E

Q

A

A

M

L

G

D

T

E

I

I

A

A

E

E

R

K

L

L

N

K

A

K

I

I

R

I

A

A

E

K

G

-

L

-

D

-

A

-

Q

Y

G

G

Y

P

P

E

K

S

F

L

A

G

A

V

S

S

F

Q

G
x
T

G
x
E

A
x
I

G
x
N

T

Q

P

Q

T

S

Q

E

Y

Y

M

-

G

G

S

T

L

L

T

R

A

R

F

F

L

M

S

N

A

L

W

L

G

G

P

S

-

P

-

N

-

W

-

T

-

S

-

A

I

M

D

Y

F

M

G
x
C

F
x
I

G

G

S

N

G

T

Q

A

G

G

V

V

-

H

K

R

C

V

Y

T

H

H

S

G

E

S

H

Y

L

S

Y

F

G

A

E

S

F

F

W

-

H

-

R

-

G

-

Y

-

V

-

I

-

T

-

P

-

D

-

T

-

P

A

R

D

A

S

N

N

Y

C

I

L

S

F

C

I

G

G

T
x
K

N
|
N

T

L

E

S

A

N

S
x
H

S
x
N

G
x
W

V

V

C

S

G

Q

V

F

Y

N

R

D

H

L

A

K

E

A

A

A

R

L

A

K

R

R

G

G

A

C

I

K

R

L

V

I

Q

V

V
x
L

E
x
D

P
|
P

H
x
R

L

R

S

T

V

K

T

V

G

A

A

E

C

M

S

A

A

D

E

I

W

W

L

L

P

P

I

L

K

R

P
x
Y

K
x
G

T
|
T

D
|
D

A

A

F

L

L

F

M

L

T

G

L

M

L

I

N

N

V

V

L

I

V

I

F

N

E

E

A

-

P

-

R

-

E

Q

R

L

L

Y

D

D

L

K

D

E

F

F

L

V

R

E

N

N

C46 (= C59) binding
K48 (= K61) binding ; mutation to A: Does not affect catalytic activity.
TEIN 111:114 (≠ GGAG 132:135) binding
C141 (≠ G157) binding
I142 (≠ F158) mutation to A: Strongly impairs catalytic activity.
KN 172:173 (≠ TN 198:199) binding
HNW 177:179 (≠ SSG 203:205) binding
LDPR 199:202 (≠ VEPH 225:228) binding
YGTD 218:221 (≠ PKTD 244:247) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 binding
12 binding
13 D→A: Almost abolishes catalytic activity.; D→E: Does not affect catalytic activity.
16 binding
296 binding
300 binding
416:418 binding
422:423 binding
442:444 binding
460 binding
465 binding
602:613 binding
606 binding
676 binding
699 binding
720 binding

7qv7S Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
24% identity, 28% coverage: 14:274/922 of query aligns to 2:241/571 of 7qv7S

query
sites
7qv7S

E

E

K

K

V

V

Q

L

T

T

Y

T

C
|
C

Y

P

Q

Y

C
|
C

V

G

A

T

G
|
G

P
x
C

D

G

L

L

M

Y

T

-

V

L

K

K

V

V

V

E

D

N

G

E

V

K

A

I

T

V

E

G

I

V

E

E

P

P

N

D

F

-

K

-

A

-

D

K

T

L

H

H

P

P

A

V

-

N

E

Q

G

G

K

E

V
x
L

C
|
C

V

I

K

K

A

G

F

Y

G

Y

L

G

I

Y

Q

K

K

Y

S

V

Y

H

T

D

P

P

H

R

R

R

I

L

L

T

T

S

P

P

M

L

K

I

R

K

T

K

N

N

P

G

K

K

K

-

G

-

K

-

D

-

Q

-

D

-

P

-

G

-

F

F

V

V

P

P

I

V

S

S

W

W

D

D

E

E

A

A

M

L

G

N

T

F

I

I

A

A

E

N

R

G

L

L

N

K

A

K

I

I

R

K

A

S

E

E

G

Y

L

G

L

S

D

D

A

A

Q

-

G

-

Y

-

P

-

K

-

F

-

A

F

A

A

S

M

F

F

G

C

G

S

A

A

G

R

T

A

P

T

T

N

Q

E

Y

D

M

N

G

Y

S

A

L

A

T

Q

A

K

F

F

L

A

S

R

A

A

W

V

G

-

P

-

I

-

D

-

F

I

G

G

F

I

G

N

S

N

G

V

Q

D

G

H

V

C

K

A

C

R

Y

L

H

H

S

A

E

P

H

T

L

V

Y

A

G

G

E

L

F

A

W

M

H

T

R

L

G

G

Y

S

-

G

V

A

I

M

T

T

P

N

D

S

T

I

P

P

R

E

-

I

-

S

-

T

-

Y

A

S

N

D

Y

V

I

I

I

F

S

I

C

I

G

G

T

S

N

N

T

T

E

A

A

E

S

C

S

H

G

P

V
x
L

C

I

G

A

V

A

Y

H

R

V

H

-

A

I

E

K

A

A

R

K

A

E

R

R

G

G

A

A

I

K

R

L

V

I

Q

V

V

A

E

D

P

P

H

R

L

M

S

N

V

A

T

M

G

V

A

H

C

K

S

A

A

D

E

I

W

W

L

L

P

R

I

V

K

P

P

S

K

G

T

Y

D

N

A

I

A

P

F

L

L

I

M

N

T

G

L

M

L

I

N

H

V

I

L

I

V

I

F

K

E

E

A

G

P

-

R

-

E

-

R

L

L

V

D

K

L

T

D

D

F

F

L

V

R

K

N

N

binding iron/sulfur cluster: C8 (= C20), C11 (= C23), G14 (= G26), C15 (≠ P27), L42 (≠ V58), C43 (= C59), L177 (≠ V206)

7bkbD Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
22% identity, 27% coverage: 16:266/922 of query aligns to 7:239/549 of 7bkbD

query
sites
7bkbD

V

V

Q

A

T

T

Y

T

C
|
C

Y

P

Q
x
Y

C
|
C

V

G

A

V

G

G

P
x
C

D

T

L

L

M

N

T

L

V

V

K

-

V

V

V

S

D

N

G

G

V

K

A

V

T

V

E

G

I

V

E

E

P

P

N

N

F

Q

K

R

A

S

A

P

D

I

T

N

H

-

P

-

A

-

E

E

G

G

K

K

V

L

C
|
C

V

P

K
|
K

A
x
G

F

V

G

T

L

C

I

W

Q

E

K

H

S

I

Y

H

T

S

P

P

H

D

R

R

I

L

L

T

T

T

P

P

M

L

K

I

R

K

T

-

N

-

P

-

K

-

G

-

K

-

D

-

Q

K

D

D

P

G

G

K

F

F

V

I

P

E

I

A

S

S

W

W

D

D

E

E

A

A

M

L

G

D

T

L

I

V

A

A

E

K

R

N

L

L

N

K

A

V

I

I

R

Y

A

D

E

K

G

H

L

G

L

P

D

K

A

G

Q

L

G

G

Y

F

P

Q

K

T

F

S

A

C

A

R

S

T

-

V

-

N

-

E

-

D

-

C

-

Y

-

I

-

F

-

Q

-

K

F

F

G

A

G

R

A

V

G

G

T

F

P

K

T

T

Q

N

Y

N

M

V

G

D

S

N

L

C

T

A

A

R

F

I

L

C

S

H

A

-

W

-

G

G

P

P

-

S

-

V

-

A

-

G

I

L

D

S

F

L

G

S

F

F

G

G

S

S

G

G

Q

A

G

A

V

T

K

N

C

G

Y

F

H

E

S

-

E

-

H

-

L

-

Y

-

G

-

E

-

F

-

W

-

H

-

R

-

G

-

Y

-

V

-

I

-

T

-

P

-

D

D

T

A

P

L

R

N

A

A

N

D

Y

L

I

I

I

L

S

I

C

W

G

G

T

S

N

N

T

A

E

V

A

E

S

A

S

H

G

P

V
x
L

C

A

G

G

V

-

Y

R

R

R

H

I

A

A

E

Q

A

A

R

K

A

K

R

K

G

G

A

I

I

Q

R

I

V

I

Q

A

V

V

E

D

P

P

H

R

L

Y

S

T

V

M

T

T

G

A

A

R

C

L

S

A

A

D

E

T

W

Y

L

V

P

R

I

F

K

N

P

P

K

S

T

T

D

H

A

I

A

A

F

L

L

A

M

N

T

S

L

M

N

Y

V

W

L

I

V

I

F

K

E

E

A

G

P

L

R

E

E

D

R

K

binding iron/sulfur cluster: C11 (= C20), Y13 (≠ Q22), C14 (= C23), C18 (≠ P27), C46 (= C59), K48 (= K61), G49 (≠ A62), L180 (≠ V206)

5ch7A Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
24% identity, 34% coverage: 70:382/922 of query aligns to 80:405/894 of 5ch7A

query
sites
5ch7A

Y

Y

T

G

P

P

H

H

R

R

I

I

L

K

T

Y

P

P

M

L

K

I

R

R

T

V

N

-

P

-

K

-

G

G

K

E

D

R

Q

G

D

E

P

G

G

K

F

W

V

R

P

R

I

A

S

T

W

W

D

E

E

E

A

A

M

L

G

D

T

M

I

I

A

A

E

D

R

K

-

C

L

V

N

D

A

T

I

I

R

K

A

N

E

H

G

A

L

P

L

D

D

C

A

I

Q

S

G

V

Y

Y

P

S

K
x
P

F

V

A
x
P

A

A

-

V

-

S

-

P

-

V

S

S

F

F

G

S

G

A

A

G

G

H

T

R

P

F

T

A

Q

H

Y

Y

M

I

G

G

S

A

L

H

T

A

-

H

A

T

F

F

L

Y

S

D

A
x
W

W
x
Y

G

G

P
x
D

I

H

D
x
P

F

T

G

G

F

Q

G

T

S

Q

G

T

Q

C

G

G

V

V

K

Q

C

G

Y

D

H

T

S

C

E

E

H

-

L

-

Y

-

G

-

E

-

F

-

W

-

H

-

R

-

G

-

Y

-

V

-

I

-

T

T

P

A

D

D

T

W

P

F

R

N

A

S

N

K

Y

Y

I

I

S

L

C

W

G
|
G

T

S

N
|
N

T

P

E

-

A

T

S
x
Q

S
x
T

G

R

V

I

C

P

G

D

V

A

Y

H

R

F

H

L

A

S

E

E

A

A

R

Q

A

L

R

N

G

G

A

A

I

K

R

I

V

V

Q

S

V
x
I

E

S

P
|
P

H
x
D

L

Y

S
x
N

V

S

T

S

G

T

A

I

C

K

S

V

A

D

E

K

W

W

L

I

P

H

I

P

K

Q

P

P

K

G

T

T

D
|
D

A

G

A

A

F

L

L

A

M

M

T

A

L

M

L

A

N

H

V

V

L

I

V

I

F

-

E

-

A

-

P

-

R

K

E

E

R

K

L

L

-

Y

D

D

L

A

D

H

F

S

L

L

R

K

N

E

T

Q

T

T

S

D

S

L

P

S

Y

Y

L

L

V

V

G

R

P

S

D

D

-

T

G

K

W

R

Y

F

L

L

R

R

D

E

P

A

E

D

T

V

-

V

-

A

-

G

-

S

-

K

H

D

K

K

P

F

L

Y

L

F

W

W

D

N

E

A

K

K

T

T

G

G

R

K

-

P

A

V

V

I

P

P

H

K

D

G

T

S

P

W

G

G

A

D

V

Q

P

P

A

E

L

K

E

K

G

G

R

S

-

P

-

V

-

G

-

F

-

L

-

G

-

R

-

N

-

T

F

F

T

A

V

F

P

P

K

K

A

G

-

Y

V

I

T

D

L

L

G

G

-

D

-

L

-

D

-

P

A

A

D

L

E

E

D

G

R

K

W

F

E

N

H

M

T

Q

D

L

V

L

E

D

G

G

V

K

T

T

A

V

F

E

T

V

K

R

F

P

I

V

E

F

H

E

V

I

-

L

-

K

-

S

-

R

-

L

-

M

K

A

G

D

Y

N

T

T

P

P

A

E

W

K

G

A

A

A

K

K

V

I

C

T

D

G

V

V

P

T

E

A

A

K

R

A

I

I

R

T

K

E

I

L

A

A

N

R

D

E

F

F

active site: P133 (≠ K126), P135 (≠ A128), W162 (≠ A150), Y163 (≠ W151), D165 (≠ P153), P167 (≠ D155)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: Y163 (≠ W151), D165 (≠ P153)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D165 (≠ P153), G196 (= G197), N198 (= N199), Q201 (≠ S203), T202 (≠ S204), I223 (≠ V225), P225 (= P227), D226 (≠ H228), N228 (≠ S230), D245 (= D247)
binding molybdenum atom: D165 (≠ P153)

Sites not aligning to the query:

active site: 71, 452, 455
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: 421, 532, 533, 534, 538, 559, 560, 561, 562, 564, 576, 579, 582, 610, 757, 759, 765, 766, 767, 830, 844, 876
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 416, 417, 418, 422, 453, 454, 756, 758, 759, 760, 763, 764, 765, 825, 877
binding molybdenum atom: 454
binding iron/sulfur cluster: 27, 31, 33, 35, 68, 69, 72
binding sulfite ion: 799, 891, 893
binding zinc ion: 76, 77

5chcA Crystal structure of the perchlorate reductase pcrab - substrate analog seo3 bound - from azospira suillum ps (see paper)
24% identity, 34% coverage: 70:382/922 of query aligns to 81:406/895 of 5chcA

query
sites
5chcA

Y

Y

T

G

P

P

H

H

R

R

I

I

L

K

T

Y

P

P

M

L

K

I

R

R

T

V

N

-

P

-

K

-

G

G

K

E

D

R

Q

G

D

E

P

G

G

K

F

W

V

R

P

R

I

A

S

T

W

W

D

E

E

E

A

A

M

L

G

D

T

M

I

I

A

A

E

D

R

K

-

C

L

V

N

D

A

T

I

I

R

K

A

N

E

H

G

A

L

P

L

D

D

C

A

I

Q

S

G

V

Y

Y

P

S

K
x
P

F

V

A
x
P

A

A

-

V

-

S

-

P

-

V

S

S

F

F

G

S

G

A

A

G

G

H

T

R

P

F

T

A

Q

H

Y

Y

M

I

G

G

S

A

L

H

T

A

-

H

A

T

F
|
F

L

Y

S

D

A
x
W

W
x
Y

G

G

P
x
D

I

H

D
x
P

F

T

G

G

F

Q

G

T

S

Q

G

T

Q

C

G

G

V

V

K

Q

C

G

Y

D

H

T

S

C

E

E

H

-

L

-

Y

-

G

-

E

-

F

-

W

-

H

-

R

-

G

-

Y

-

V

-

I

-

T

T

P

A

D

D

T

W

P

F

R

N

A

S

N

K

Y

Y

I

I

S

L

C

W

G
|
G

T

S

N

N

T

P

E

-

A

T

S
x
Q

S
x
T

G

R

V

I

C

P

G

D

V

A

Y

H

R

F

H

L

A

S

E

E

A

A

R

Q

A

L

R

N

G

G

A

A

I

K

R

I

V

V

Q

S

V
x
I

E

S

P
|
P

H
x
D

L

Y

S
x
N

V

S

T

S

G

T

A

I

C

K

S

V

A

D

E

K

W

W

L

I

P

H

I

P

K

Q

P

P

K

G

T

T

D
|
D

A

G

A

A

F

L

L

A

M

M

T

A

L

M

L

A

N

H

V

V

L

I

V

I

F

-

E

-

A

-

P

-

R

K

E

E

R

K

L

L

-

Y

D

D

L

A

D

H

F

S

L

L

R

K

N

E

T

Q

T

T

S

D

S

L

P

S

Y

Y

L

L

V

V

G

R

P

S

D

D

-

T

G

K

W

R

Y

F

L

L

R

R

D

E

P

A

E

D

T

V

-

V

-

A

-

G

-

S

-

K

H

D

K

K

P

F

L

Y

L

F

W

W

D

N

E

A

K

K

T

T

G

G

R

K

-

P

A

V

V

I

P

P

H

K

D

G

T

S

P

W

G

G

A

D

V

Q

P

P

A

E

L

K

E

K

G

G

R

S

-

P

-

V

-

G

-

F

-

L

-

G

-

R

-

N

-

T

F

F

T

A

V

F

P

P

K

K

A

G

-

Y

V

I

T

D

L

L

G

G

-

D

-

L

-

D

-

P

A

A

D

L

E

E

D

G

R

K

W

F

E

N

H

M

T

Q

D

L

V

L

E

D

G

G

V

K

T

T

A

V

F

E

T

V

K

R

F

P

I

V

E

F

H

E

V

I

-

L

-

K

-

S

-

R

-

L

-

M

K

A

G

D

Y

N

T

T

P

P

A

E

W

K

G

A

A

A

K

K

V

I

C

T

D

G

V

V

P

T

E

A

A

K

R

A

I

I

R

T

K

E

I

L

A

A

N

R

D

E

F

F

active site: P134 (≠ K126), P136 (≠ A128), W163 (≠ A150), Y164 (≠ W151), D166 (≠ P153), P168 (≠ D155)
binding biselenite ion: F160 (= F147), Y164 (≠ W151), D166 (≠ P153)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: Y164 (≠ W151), D166 (≠ P153)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D166 (≠ P153), G197 (= G197), Q202 (≠ S203), T203 (≠ S204), I224 (≠ V225), P226 (= P227), D227 (≠ H228), N229 (≠ S230), D246 (= D247)
binding molybdenum atom: D166 (≠ P153)

Sites not aligning to the query:

active site: 72, 453, 456
binding biselenite ion: 455
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: 422, 533, 534, 535, 539, 560, 561, 563, 565, 580, 611, 758, 760, 766, 767, 768, 831, 845, 877
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 417, 418, 419, 423, 454, 455, 757, 759, 760, 761, 764, 765, 766, 826, 878
binding molybdenum atom: 455
binding iron/sulfur cluster: 28, 32, 34, 36, 69, 70, 73

2iv2X Reinterpretation of reduced form of formate dehydrogenase h from e. Coli (see paper)
31% identity, 14% coverage: 13:141/922 of query aligns to 1:119/697 of 2iv2X

query
sites
2iv2X

V

M

E

K

K

K

V

V

Q

V

T

T

Y

V

C
|
C

Y

P

Q

Y

C
|
C

V

A

A
x
S

G

G

P
x
C

D

K

L

I

M

N

T

L

V

V

K

-

V

V

V

D

D

N

G

G

V

K

A

I

T

V

E

R

I

A

E

E

P

-

N

-

F

-

K

-

A

A

D

Q

T

G

H

K

P

T

A

N

E

Q

G

G

K

T

V
x
L

C
|
C

V

L

K
|
K

A
x
G

F

Y

-

Y

-

G

-

W

G

D

L

F

I

I

Q

N

K

D

S

T

Y

-

T

-

P

-

H

-

R

Q

I

I

L

L

T

T

P

P

M

R

K

L

R

K

T

T

N

-

P

P

K

M

K

I

G

R

K

R

D

Q

Q

R

D

G

P

G

G

K

F

L

V

E

P

P

I

V

S

S

W

W

D

D

E

E

A

A

M

L

G

N

T

Y

I

V

A

A

E

E

R

R

L

L

N

S

A

A

I

I

R

K

A

E

E

K

G

Y

L

G

L

P

D

D

A

A

Q

-

G

-

Y

-

P

I

K

Q

F

T

A

T

A

G

S
|
S

F

S

G
x
R

G
|
G

A
x
T

G

G

T

N

P

E

T

T

Q

N

Y

Y

M

V

active site: C11 (= C23), L41 (≠ V58), C42 (= C59), K44 (= K61), S108 (= S130)
binding guanylate-o'-phosphoric acidmono-(2-amino-5,6-dimercapto-4-oxo-3,5,6,7,8a,9,10,10a-octahydro-4h-8-oxa-1,3,9,10-tetraaza-anthracen-7-ylmethyl) ester: R110 (≠ G132), G111 (= G133), T112 (≠ A134)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K44 (= K61)
binding iron/sulfur cluster: C8 (= C20), C11 (= C23), S13 (≠ A25), C15 (≠ P27), L41 (≠ V58), C42 (= C59), K44 (= K61), G45 (≠ A62)

Sites not aligning to the query:

active site: 140, 141, 180, 297, 333, 334, 335
binding guanylate-o'-phosphoric acidmono-(2-amino-5,6-dimercapto-4-oxo-3,5,6,7,8a,9,10,10a-octahydro-4h-8-oxa-1,3,9,10-tetraaza-anthracen-7-ylmethyl) ester: 137, 335, 402, 403, 404, 408, 410, 428, 429, 430, 445, 478, 588, 660
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 173, 174, 175, 176, 179, 180, 202, 203, 204, 223, 296, 297, 302, 334, 581, 582, 583, 585, 586, 587, 661
binding iron/sulfur cluster: 183

1fdiA Oxidized form of formate dehydrogenase h from e. Coli complexed with the inhibitor nitrite (see paper)
31% identity, 14% coverage: 13:141/922 of query aligns to 1:119/715 of 1fdiA

query
sites
1fdiA

V

M

E

K

K

K

V

V

Q

V

T

T

Y

V

C
|
C

Y

P

Q
x
Y

C
|
C

V

A

A
x
S

G

G

P
x
C

D

K

L

I

M

N

T

L

V

V

K

-

V

V

V

D

D

N

G

G

V

K

A

I

T

V

E

R

I

A

E

E

P

-

N

-

F

-

K

-

A

A

D

Q

T

G

H

K

P

T

A

N

E

Q

G

G

K

T

V
x
L

C
|
C

V

L

K
|
K

A

G

F

Y

-

Y

-

G

-

W

G

D

L

F

I

I

Q

N

K

D

S

T

Y

-

T

-

P

-

H

-

R

Q

I

I

L

L

T

T

P

P

M

R

K

L

R

K

T

T

N

-

P

P

K

M

K

I

G

R

K

R

D

Q

Q

R

D

G

P

G

G

K

F

L

V

E

P

P

I

V

S

S

W

W

D

D

E

E

A

A

M

L

G

N

T

Y

I

V

A

A

E

E

R

R

L

L

N

S

A

A

I

I

R

K

A

E

E

K

G

Y

L

G

L

P

D

D

A

A

Q

-

G

-

Y

-

P

I

K

Q

F

T

A

T

A

G

S
|
S

F

S

G
x
R

G
|
G

A

T

G

G

T

N

P

E

T

T

Q

N

Y

Y

M

V

active site: C11 (= C23), L41 (≠ V58), C42 (= C59), K44 (= K61), S108 (= S130), R110 (≠ G132)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K44 (= K61), R110 (≠ G132), G111 (= G133)
binding iron/sulfur cluster: C8 (= C20), Y10 (≠ Q22), C11 (= C23), S13 (≠ A25), C15 (≠ P27), L41 (≠ V58), C42 (= C59), K44 (= K61)

Sites not aligning to the query:

active site: 134, 140, 141, 180, 297, 333, 334, 335
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 139, 140, 173, 174, 175, 176, 179, 180, 201, 202, 203, 204, 218, 221, 223, 296, 297, 298, 302, 334, 335, 335, 402, 403, 408, 428, 429, 430, 445, 478, 579, 580, 581, 581, 582, 585, 586, 587, 588, 654, 662, 678, 679
binding nitrite ion: 140, 141, 333, 334, 338
binding iron/sulfur cluster: 182, 183

P07658 Formate dehydrogenase H; Formate dehydrogenase-H subunit alpha; FDH-H; Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide; EC 1.17.98.4 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 14% coverage: 13:141/922 of query aligns to 1:119/715 of P07658

query
sites
P07658

V

M

E

K

K

K

V

V

Q

V

T

T

Y

V

C
|
C

Y

P

Q
x
Y

C
|
C

V

A

A

S

G

G

P
x
C

D

K

L

I

M

N

T

L

V

V

K

-

V

V

V

D

D

N

G

G

V

K

A

I

T

V

E

R

I

A

E

E

P

-

N

-

F

-

K

-

A

A

D

Q

T

G

H

K

P

T

A

N

E

Q

G

G

K

T

V

L

C
|
C

V

L

K
|
K

A

G

F

Y

-

Y

-

G

-

W

G

D

L

F

I

I

Q

N

K

D

S

T

Y

-

T

-

P

-

H

-

R

Q

I

I

L

L

T

T

P

P

M

R

K

L

R

K

T

T

N

-

P

P

K

M

K

I

G

R

K

R

D

Q

Q

R

D

G

P

G

G

K

F

L

V

E

P

P

I

V

S

S

W

W

D

D

E

E

A

A

M

L

G

N

T

Y

I

V

A

A

E

E

R

R

L

L

N

S

A

A

I

I

R

K

A

E

E

K

G

Y

L

G

L

P

D

D

A

A

Q

-

G

-

Y

-

P

I

K

Q

F

T

A

T

A

G

S

S

F

S

G

R

G

G

A

T

G

G

T

N

P

E

T

T

Q

N

Y

Y

M

V

C8 (= C20) binding
Y10 (≠ Q22) binding
C11 (= C23) binding
C15 (≠ P27) binding
C42 (= C59) binding
K44 (= K61) binding

Sites not aligning to the query:

140 modified: nonstandard, Selenocysteine
297 binding
301 binding
335 binding
445 binding
478 binding
588 binding
654 binding
655 binding
678 binding
679 binding

Query Sequence

>WP_011383653.1 NCBI__GCF_000009985.1:WP_011383653.1
MSAAPAGGGGKKVEKVQTYCYQCVAGPDLMTVKVVDGVATEIEPNFKAADTHPAEGKVCV
KAFGLIQKSYTPHRILTPMKRTNPKKGKDQDPGFVPISWDEAMGTIAERLNAIRAEGLLD
AQGYPKFAASFGGAGTPTQYMGSLTAFLSAWGPIDFGFGSGQGVKCYHSEHLYGEFWHRG
YVITPDTPRANYIISCGTNTEASSGVCGVYRHAEARARGAIRVQVEPHLSVTGACSAEWL
PIKPKTDAAFLMTLLNVLVFEAPRERLDLDFLRNTTSSPYLVGPDGWYLRDPETHKPLLW
DEKTGRAVPHDTPGAVPALEGRFTVPKAVTLGADEDRWEHTDVEGVTAFTKFIEHVKGYT
PAWGAKVCDVPEARIRKIANDFLDHACVGQTIEIDGQVMPYRPVAVVLGKTVNNGWGGAE
CCWARTMLAVLVGALEVPGGTVGTTIRINRPAANRLESFEGCLDGFMEYPFNPTDKDTWK
ANPIIRNAYKMLVPLVGNSSWSPALGPTQFSYMFLDEPQDQIPRATFPEFLLVYRTNPVI
SFWDTDRVASVVSRMPFVVCFAHTRDETNHFADILLPDATDLEGMQLIRIGGTKFQEQFW
KQQGFALRQPSVKPQGEARDFTDIATDLAVRTGLQEKYVAAINRGSHGVPLKGPNWDFSL
PVDKAPSLEEVWDASCRSASAELSDGAESQGLDWWKENGFRTIPFSETNWFLTPALKAKG
LRYELPYQERLTRIGRQLANRLKEAGITWWDHQLTEYRPLMEWHDFPGYWEQSVVEHGGK
VADFPFWVVTARSMQYAWGSNMHIPLMREVSGHIKGHDGVVMNPEAARKVGVKEGERIVV
TAPTGKSVAGRVVLSQGIRPDTILMMSQFDHWATPIAKDFDVPSMNRLTAMTMQLTDATG
SAADLSRVAIRKARADEQGRRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory