SitesBLAST
Comparing WP_011383654.1 NCBI__GCF_000009985.1:WP_011383654.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
37% identity, 97% coverage: 1:211/217 of query aligns to 1:178/193 of 2vpyF
- binding pentachlorophenol: I91 (= I91), C93 (= C93)
- binding iron/sulfur cluster: C13 (= C13), G15 (= G15), C16 (= C16), C19 (= C19), C23 (= C23), N27 (= N27), L36 (≠ R36), I38 (≠ V38), P55 (= P55), Q57 (≠ G57), C58 (= C58), L59 (≠ M59), C61 (= C61), P65 (= P65), C66 (= C66), C70 (= C70), P71 (= P71), V83 (= V83), C90 (= C90), I91 (= I91), A92 (≠ G92), C93 (= C93), G94 (= G94), C96 (= C96), C100 (= C100), P101 (= P101), Y102 (= Y102), R105 (= R105), V113 (≠ A135), C116 (= C138), F118 (= F140), C119 (= C141), P129 (= P163), A130 (= A164), C131 (= C165), C135 (= C169), C139 (≠ A173), R140 (≠ L174)
Sites not aligning to the query:
2vpwF Polysulfide reductase with bound menaquinone (see paper)
37% identity, 97% coverage: 1:211/217 of query aligns to 1:178/193 of 2vpwF
- binding iron/sulfur cluster: C13 (= C13), G15 (= G15), C16 (= C16), C19 (= C19), C23 (= C23), N27 (= N27), L36 (≠ R36), I38 (≠ V38), P55 (= P55), Q57 (≠ G57), C58 (= C58), L59 (≠ M59), H60 (= H60), C61 (= C61), P65 (= P65), C66 (= C66), C70 (= C70), P71 (= P71), V83 (= V83), C90 (= C90), I91 (= I91), A92 (≠ G92), C93 (= C93), G94 (= G94), C96 (= C96), C100 (= C100), P101 (= P101), Y102 (= Y102), R105 (= R105), V113 (≠ A135), C116 (= C138), F118 (= F140), C119 (= C141), P129 (= P163), A130 (= A164), C131 (= C165), C135 (= C169), C139 (≠ A173), R140 (≠ L174)
Sites not aligning to the query:
6cz7B The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
34% identity, 96% coverage: 3:211/217 of query aligns to 2:225/234 of 6cz7B
- binding iron/sulfur cluster: C12 (= C13), V13 (= V14), G14 (= G15), C15 (= C16), G16 (≠ Q17), C18 (= C19), C22 (= C23), N26 (= N27), Y52 (≠ F53), P54 (= P55), C57 (= C58), N58 (≠ M59), H59 (= H60), C60 (= C61), A63 (≠ P64), P64 (= P65), C65 (= C66), C69 (= C70), P70 (= P71), T82 (≠ V83), C89 (= C90), I90 (= I91), G91 (= G92), C92 (= C93), K93 (≠ G94), C95 (= C96), C99 (= C100), Y101 (= Y102), V103 (≠ A104), I104 (vs. gap), T161 (≠ A135), K163 (= K137), C164 (= C138), F166 (= F140), C167 (= C141), C179 (= C165), C183 (= C169), P184 (≠ L170), R188 (≠ L174)
Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
34% identity, 96% coverage: 3:211/217 of query aligns to 2:225/234 of Q7WTT9
- C12 (= C13) binding
- C15 (= C16) binding
- C18 (= C19) binding
- C22 (= C23) binding
- C57 (= C58) binding
- C60 (= C61) binding
- C65 (= C66) binding
- C69 (= C70) binding
- C89 (= C90) binding
- C92 (= C93) binding
- C95 (= C96) binding
- C99 (= C100) binding
- C164 (= C138) binding
- C167 (= C141) binding
- C179 (= C165) binding
- C183 (= C169) binding
6f0kB Alternative complex iii (see paper)
33% identity, 97% coverage: 2:211/217 of query aligns to 722:948/961 of 6f0kB
- binding fe3-s4 cluster: C803 (= C70), V805 (≠ T72), M818 (≠ I85), C823 (= C90), I824 (= I91), G825 (= G92), C829 (= C96), M869 (≠ A135)
- binding heme c: N821 (≠ D88), R822 (≠ I89), R884 (≠ A150), N887 (vs. gap)
- binding iron/sulfur cluster: C733 (= C13), T734 (≠ V14), G735 (= G15), C736 (= C16), N737 (≠ Q17), A738 (≠ T18), C739 (= C19), C743 (= C23), W765 (≠ F46), I768 (≠ V49), C791 (= C58), M792 (= M59), H793 (= H60), C794 (= C61), P798 (= P65), C799 (= C66), N816 (≠ V83), C833 (= C100), C872 (= C138), Y874 (≠ F140), C875 (= C141), A901 (= A164), C902 (= C165), C906 (= C169)
Sites not aligning to the query:
P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
33% identity, 95% coverage: 2:208/217 of query aligns to 3:181/205 of P18776
- C102 (= C93) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
33% identity, 79% coverage: 40:211/217 of query aligns to 115:255/329 of 5ch7B
- binding fe3-s4 cluster: C145 (= C70), I150 (≠ T75), C166 (= C90), K167 (≠ I91), G168 (= G92), A169 (≠ C93), Q170 (≠ G94), A171 (≠ Y95), C172 (= C96), A190 (= A135)
- binding iron/sulfur cluster: P130 (= P55), C133 (= C58), N134 (≠ M59), C136 (= C61), P139 (= P64), C141 (= C66), C176 (= C100), C193 (= C138), G195 (≠ F140), C196 (= C141), C208 (= C165), C212 (= C169), V213 (≠ L170), G214 (≠ S171)
Sites not aligning to the query:
6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
28% identity, 96% coverage: 3:211/217 of query aligns to 682:917/929 of 6lodB
- binding fe3-s4 cluster: V758 (= V69), C759 (= C70), P760 (= P71), C779 (= C90), V780 (≠ I91), G781 (= G92), T782 (≠ C93), C785 (= C96), M838 (≠ A135)
- binding heme c: N777 (≠ D88), R778 (≠ I89), R852 (≠ L149), I853 (≠ A150), R856 (≠ L153)
- binding iron/sulfur cluster: C692 (= C13), N693 (≠ V14), S694 (≠ G15), C695 (= C16), N696 (≠ Q17), A697 (≠ T18), C698 (= C19), C702 (= C23), N706 (= N27), W724 (≠ Y35), I727 (≠ V38), L746 (≠ P55), C747 (= C58), Q748 (≠ M59), Q749 (≠ H60), C750 (= C61), P754 (= P65), C755 (= C66), N772 (≠ V83), C789 (= C100), Y791 (= Y102), V793 (≠ A104), R794 (= R105), K840 (= K137), C841 (= C138), F843 (= F140), C844 (= C141), T869 (≠ P163), C871 (= C165), C875 (= C169), I880 (≠ L174)
2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
32% identity, 83% coverage: 31:211/217 of query aligns to 109:258/337 of 2ivfB
- binding fe3-s4 cluster: C148 (= C70), T150 (= T72), I153 (≠ T75), C169 (= C90), K170 (≠ I91), G171 (= G92), H172 (≠ C93), R173 (≠ G94), H174 (≠ Y95), C175 (= C96), S193 (≠ A135)
- binding protoporphyrin ix containing fe: T150 (= T72), K170 (≠ I91), H172 (≠ C93)
- binding iron/sulfur cluster: M135 (≠ G57), C136 (= C58), N137 (≠ M59), H138 (= H60), C139 (= C61), P142 (= P64), C144 (= C66), V162 (= V83), C179 (= C100), A183 (= A104), I184 (≠ R105), K195 (= K137), C196 (= C138), I197 (≠ T139), L198 (≠ F140), C199 (= C141), N209 (= N151), C211 (= C165), C215 (= C169), V219 (≠ A173), R220 (≠ L174)
Sites not aligning to the query:
- binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41
6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
27% identity, 93% coverage: 9:210/217 of query aligns to 689:942/948 of 6btmB
- binding fe3-s4 cluster: C789 (= C70), C809 (= C90), V810 (≠ I91), T812 (≠ C93), C815 (= C96), M862 (≠ A135)
- binding iron/sulfur cluster: C777 (= C58), Q778 (≠ M59), C780 (= C61), C785 (= C66), N802 (≠ V83), C819 (= C100), R824 (= R105)
Sites not aligning to the query:
7qv7A Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
29% identity, 92% coverage: 6:204/217 of query aligns to 5:172/174 of 7qv7A
- binding iron/sulfur cluster: C12 (= C13), L13 (≠ V14), G14 (= G15), C15 (= C16), Y16 (≠ Q17), C18 (= C19), C22 (= C23), H26 (≠ N27), L37 (≠ V49), C51 (= C58), R52 (≠ M59), C54 (= C61), C59 (= C66), C63 (= C70), C82 (= C90), I83 (= I91), G84 (= G92), C85 (= C93), C88 (= C96), C92 (= C100), I97 (≠ P111), F99 (= F113), C125 (= C138), L127 (≠ F140), C128 (= C141), A136 (= A164), C137 (= C165), C141 (= C169)
1kqfB Formate dehydrogenase n from e. Coli (see paper)
28% identity, 98% coverage: 5:217/217 of query aligns to 30:232/289 of 1kqfB
- binding protoporphyrin ix containing fe: Y137 (= Y95)
- binding iron/sulfur cluster: K31 (≠ I6), C38 (= C13), I39 (≠ V14), G40 (= G15), C41 (= C16), K42 (≠ Q17), C44 (= C19), C48 (= C23), N52 (= N27), T77 (≠ P47), M79 (≠ V49), C99 (= C58), M100 (= M59), H101 (= H60), C102 (= C61), P105 (= P64), C107 (= C66), C111 (= C70), P112 (= P71), I117 (≠ T75), V125 (= V83), C132 (= C90), I133 (= I91), G134 (= G92), C135 (= C93), G136 (= G94), Y137 (= Y95), C138 (= C96), C142 (= C100), I146 (≠ A104), P147 (= P111), V156 (≠ L131), K158 (= K137), C159 (= C138), L161 (≠ F140), C162 (= C141), P172 (= P163), C174 (= C165), C178 (= C169), P179 (≠ L170), I183 (≠ L174)
Sites not aligning to the query:
P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
28% identity, 98% coverage: 5:217/217 of query aligns to 31:233/294 of P0AAJ3
- C39 (= C13) binding
- C42 (= C16) binding
- C45 (= C19) binding
- C49 (= C23) binding
- C100 (= C58) binding
- C103 (= C61) binding
- C108 (= C66) binding
- C112 (= C70) binding
- C133 (= C90) binding
- C136 (= C93) binding
- C139 (= C96) binding
- C143 (= C100) binding
- C160 (= C138) binding
- C163 (= C141) binding
- C175 (= C165) binding
- C179 (= C169) binding
7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
28% identity, 79% coverage: 6:176/217 of query aligns to 7:161/183 of 7qv7B
- binding iron/sulfur cluster: C14 (= C13), G16 (= G15), C17 (= C16), K18 (≠ Q17), A19 (≠ T18), C20 (= C19), C24 (= C23), H28 (vs. gap), R48 (≠ Y35), C63 (= C58), R64 (≠ M59), H65 (= H60), C66 (= C61), A69 (≠ P64), C71 (= C66), C75 (= C70), A79 (≠ T75), I80 (≠ T76), C94 (= C90), I95 (= I91), C97 (= C93), C100 (= C96), C104 (= C100), I109 (vs. gap), C139 (= C138), L141 (≠ F140), C142 (= C141), P148 (= P163), C150 (= C165), C154 (= C169), P155 (≠ L170), L159 (= L174)
7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
30% identity, 78% coverage: 42:211/217 of query aligns to 156:305/409 of 7b04A
- binding fe3-s4 cluster: C186 (= C70), I191 (≠ T75), C207 (= C90), R208 (≠ I91), G209 (= G92), Y210 (≠ C93), R211 (≠ G94), K212 (≠ Y95), C213 (= C96), S231 (≠ A135)
- binding protoporphyrin ix containing fe: R188 (≠ T72), R208 (≠ I91), Y210 (≠ C93)
- binding iron/sulfur cluster: Q171 (≠ G57), I173 (vs. gap), C174 (= C58), N175 (≠ M59), H176 (= H60), C177 (= C61), P180 (= P64), C182 (= C66), C217 (= C100), P222 (= P111), K233 (= K137), C234 (= C138), A236 (≠ F140), C237 (= C141), T255 (≠ A161), R256 (≠ A164), C257 (= C165), C261 (= C169), V262 (≠ L170), I265 (≠ A173), R266 (≠ L174)
Sites not aligning to the query:
- binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62
4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
26% identity, 97% coverage: 1:210/217 of query aligns to 1:188/274 of 4v4cB
- binding calcium ion: I61 (≠ R51), N62 (≠ V52)
- binding iron/sulfur cluster: C13 (= C13), Q14 (≠ V14), D15 (≠ G15), C16 (= C16), N17 (≠ Q17), N18 (≠ T18), C19 (= C19), C23 (= C23), C68 (= C58), M69 (= M59), H70 (= H60), C71 (= C61), C76 (= C66), V92 (= V83), C109 (= C100), V113 (≠ A104), C126 (= C138), M128 (≠ F140), C129 (= C141), P143 (= P163), C145 (= C165), C149 (= C169), S151 (= S171), V153 (≠ A173), Y154 (≠ L174)
7z0sB Structure of the escherichia coli formate hydrogenlyase complex (anaerobic preparation, without formate dehydrogenase h) (see paper)
28% identity, 80% coverage: 6:179/217 of query aligns to 5:169/170 of 7z0sB
- binding iron/sulfur cluster: C12 (= C13), I13 (≠ V14), G14 (= G15), C15 (= C16), H16 (≠ Q17), T17 (= T18), C18 (= C19), C22 (= C23), H26 (≠ N27), R36 (≠ V38), L37 (= L39), P48 (= P55), L50 (≠ G57), C51 (= C58), H52 (≠ M59), H53 (= H60), C54 (= C61), C59 (= C66), C63 (= C70), P64 (= P71), I68 (≠ T75), V75 (= V83), L77 (≠ I85), C82 (= C90), V83 (≠ I91), C85 (= C93), K86 (≠ G94), C88 (= C96), C92 (= C100), I97 (vs. gap), A140 (= A135), K142 (= K137), C143 (= C138), D144 (≠ T139), L145 (≠ F140), C146 (= C141), P153 (= P163), C155 (= C165), C159 (= C169), P160 (≠ L170), T161 (≠ S171), L164 (= L174)
P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 47% coverage: 46:148/217 of query aligns to 172:254/512 of P11349
- C184 (= C58) binding
- C187 (= C61) binding
- C192 (= C66) binding
- C196 (= C70) binding
- C217 (= C90) binding
- C223 (= C96) binding
- C227 (= C100) binding
- C244 (= C138) binding
- C247 (= C141) binding
Sites not aligning to the query:
- 16 binding
- 19 binding
- 22 binding
- 26 binding
- 259 binding
- 263 binding
3egwB The crystal structure of the narghi mutant narh - c16a
34% identity, 47% coverage: 46:148/217 of query aligns to 172:254/509 of 3egwB
- binding fe3-s4 cluster: P181 (= P55), C196 (= C70), S198 (≠ T72), I201 (≠ T75), C217 (= C90), R218 (≠ I91), G219 (= G92), W220 (≠ C93), R221 (≠ G94), C223 (= C96), S241 (≠ A135)
- binding protoporphyrin ix containing fe: W220 (≠ C93), R221 (≠ G94)
- binding iron/sulfur cluster: C184 (= C58), E185 (≠ M59), H186 (= H60), C187 (= C61), P190 (= P64), C192 (= C66), C227 (= C100), I232 (≠ R105), K243 (= K137), C244 (= C138), F246 (= F140), C247 (= C141)
Sites not aligning to the query:
- binding fe3-s4 cluster: 17, 18, 19, 20, 22, 263, 265, 268
- binding protoporphyrin ix containing fe: 88, 89
- binding iron/sulfur cluster: 26, 30, 41, 42, 257, 259
Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
26% identity, 90% coverage: 1:195/217 of query aligns to 1:183/215 of Q8GC87
- C12 (= C13) binding
- C15 (= C16) binding
- C18 (= C19) binding
- C22 (= C23) binding
- C73 (= C58) binding
- C76 (= C61) binding
- C81 (= C66) binding
- C121 (= C100) binding
- C138 (= C138) binding
- C141 (= C141) binding
- C153 (= C165) binding
- C157 (= C169) binding
Query Sequence
>WP_011383654.1 NCBI__GCF_000009985.1:WP_011383654.1
MTKWAIVADLGRCVGCQTCTTACRHANATPPGVQYRKVLDMEVGTFPDVRRVFVPVGCMH
CDEPPCRDVCPTTATTKRADGMVMIDYDICIGCGYCIVACPYQARYKVSKPTFAFKDQET
QNERTRFDERLLGVAQKCTFCVDRVDYGLANGLTPGVAPEATPACVNSCLSKALTFGDTD
NPDSNVSKLLKRYKSFRMHEELGTGPNIHYLWETEDE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory