SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011383723.1 AMB_RS06675 membrane protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5uhsA Structure of a semisweet d57a mutant (see paper)
49% identity, 82% coverage: 8:84/94 of query aligns to 3:79/81 of 5uhsA

query
sites
5uhsA

D

N

L

L

L

I

G

G

A

Y

A

V

A

A

G

A

V

F

L

L

T

T

T

T

I

V

S

S

F

F

V

L

P

P

Q

Q

V

V

V

L

K

R

T

V

L

V

R

M

T

T

R

K

Q

Q

T

T

R

R

D

D

I

I

S

S

L

R

A

N

M

M

W

Y

V

I

C

M

F
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F

I

F

T

L

G

G

V

V

S

V

L

L

W
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W

T

F

V

V

Y

Y

G

G

L

I

L

L

L

R

G

S

A

A

W

L

P

P

I

I

V

I

A

L

S

A

N

N

L

V

P

V

T

T

L

L

G

F

L

F

A

V

G

T

T

I

I

I

L

L

V

Y

I

Y

K

K

L

L

binding beta-D-glucopyranose: F41 (= F46), W48 (= W53)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
46% identity, 89% coverage: 8:91/94 of query aligns to 3:85/85 of B0SR19

query
sites
B0SR19

D

N

L

L

L

I

G

G

A

Y

A

V

A

A

G

A

V

F

L

L

T

T

T

T

I

V

S

S

F

F

V

L

P

P

Q

Q

V

V

V

L

K

R

T

V

L

V

R

M

T

T

R

K

Q

Q

T

T

R

R

D

D

I

I

S

S

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A

N

M

M

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Y

V

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C

M

F

F

I

F

T

L

G

G

V

V

S

V

L

L

W
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W

T

F

V

V

Y

Y

G

G

L

I

L

L

L

R

G

S

A

D

W

L

P

P

I

I

V

I

A

L

S

A

N
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N

L

V

P

V

T

T

L

L

G

F

L

F

A

V

G

T

T

I

I

I

L

L

V

Y

I

Y

K

K

L

L

R

T

N

E

M

-

G

G

K

N

E

Q

T

T

W48 (= W53) mutation to A: Impairs glucose transport.
N64 (= N69) mutation to A: Impairs glucose transport.

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
43% identity, 71% coverage: 24:90/94 of query aligns to 21:87/89 of P0DMV3

query
sites
P0DMV3

P
|
P

Q
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Q

V

S

V

I

K

K

T

T

L

I

R

R

T

T

R

R

Q

N

T

T

R

E

D

G

I

I

S

S

L

V

A

V

M
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M

W
x
Y

V

I

C

M

F

F

I

L

T

T

G

G

V

V

S

I

L

S

W
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W

T

I

V

A

Y
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Y

G

G

L

I

L

M

L
x
R

G

S

A

D

W

F

P

A

I

V

V

L

A

I

S

A

N
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N

L

I

P

V

T

T

L

L

G

F

L

L

A

A

G

A

T

P

I

V

L

L

V

V

I

I

K

T

L

L

R

I

N

N

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K

E

K

P21 (= P24) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q25) mutation to A: Slightly decreases sucrose transporter activity.
M39 (= M42) mutation to A: No effect on sucrose transporter activity.
Y40 (≠ W43) mutation to A: Decreases sucrose transporter activity.
W50 (= W53) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y56) mutation to F: Increases sucrose transporter activity.
R57 (≠ L60) mutation to A: Increases sucrose transporter activity.
N66 (= N69) mutation to A: Impairs sucrose transporter activity.

Sites not aligning to the query:

19 F→A: Decreases sucrose transporter activity.

Query Sequence

>WP_011383723.1 AMB_RS06675 membrane protein
MDWLSPIDLLGAAAGVLTTISFVPQVVKTLRTRQTRDISLAMWVCFITGVSLWTVYGLLL
GAWPIVASNLPTLGLAGTILVIKLRNMGKETGGD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory