SitesBLAST

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
38% identity, 61% coverage: 145:428/469 of query aligns to 14:301/310 of 5exdF

query
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active site: N143 (= N270)
binding magnesium ion: D110 (= D237), N138 (= N265), S140 (≠ G267)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ P181), G51 (= G182), C52 (= C183), I74 (≠ L207), D110 (= D237), G111 (= G238), Y136 (≠ V263), N138 (= N265), S140 (≠ G267), Y141 (= Y268), A142 (≠ M269), N143 (= N270), T144 (= T271)
binding iron/sulfur cluster: C24 (= C155), C27 (= C158), P29 (≠ S160), C52 (= C183), A142 (≠ M269), C225 (= C351), K227 (≠ T353)

8c0zC Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
34% identity, 28% coverage: 5:134/469 of query aligns to 2:136/158 of 8c0zC

query
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8c0zC

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binding iron/sulfur cluster: C12 (= C15), T13 (≠ D16), C15 (= C18), C18 (= C21), C22 (= C25), C53 (= C51), C56 (= C54), C61 (= C59), C65 (= C63), I70 (≠ L68), C86 (= C84), C89 (= C87), C92 (= C90), C96 (= C94), T100 (≠ G98), K112 (= K110), C113 (= C111), C116 (= C114), C122 (= C120), C126 (= C124), T128 (= T126)

P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
29% identity, 26% coverage: 10:131/469 of query aligns to 34:186/294 of P0AAJ3

query
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P0AAJ3

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C39 (= C15) binding
C42 (= C18) binding
C45 (= C21) binding
C49 (= C25) binding
C100 (= C51) binding
C103 (= C54) binding
C108 (= C59) binding
C112 (= C63) binding
C133 (= C84) binding
C136 (= C87) binding
C139 (= C90) binding
C143 (= C94) binding
C160 (= C111) binding
C163 (= C114) binding
C175 (= C120) binding
C179 (= C124) binding

1kqfB Formate dehydrogenase n from e. Coli (see paper)
29% identity, 26% coverage: 10:131/469 of query aligns to 33:185/289 of 1kqfB

query
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binding protoporphyrin ix containing fe: Y137 (≠ L89)
binding iron/sulfur cluster: C38 (= C15), I39 (≠ D16), G40 (= G17), C41 (= C18), K42 (≠ G19), C44 (= C21), C48 (= C25), N52 (≠ K29), T77 (vs. gap), M79 (vs. gap), C99 (= C51), M100 (≠ R52), H101 (≠ Q53), C102 (= C54), P105 (= P57), C107 (= C59), C111 (= C63), P112 (= P64), I117 (≠ D69), V125 (≠ I77), C132 (= C84), I133 (≠ V85), G134 (≠ D86), C135 (= C87), G136 (≠ L88), Y137 (≠ L89), C138 (= C90), C142 (= C94), I146 (≠ G98), P147 (≠ I99), V156 (= V108), K158 (= K110), C159 (= C111), L161 (≠ Q113), C162 (= C114), P172 (= P118), C174 (= C120), C178 (= C124), P179 (= P125), I183 (≠ L129)

Sites not aligning to the query:

P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
29% identity, 28% coverage: 12:142/469 of query aligns to 11:163/205 of P18776

query
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P18776

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C102 (= C87) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7plmA Cryoem reconstruction of pyruvate ferredoxin oxidoreductase (pfor) in anaerobic conditions (see paper)
28% identity, 44% coverage: 231:435/469 of query aligns to 924:1129/1177 of 7plmA

query
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P

D

E

Q

D

L

A

A

K

H

R

I

L

Q

R

A

A

K

Q

V

V

-

A

-

H

-

E

-

L

D

D

E

V

Q

R

V

F

A

K

L

E

L

L

K

E

L

H

M

M

A

A

binding calcium ion: D950 (≠ K257), N952 (≠ L259), F1026 (= F339), G1028 (= G341)
binding magnesium ion: D930 (= D237), T958 (≠ N265), V960 (≠ G267), S962 (≠ M269)
binding iron/sulfur cluster: S962 (≠ M269), C1038 (= C351), I1039 (≠ P352)
binding thiamine diphosphate: G929 (= G236), D930 (= D237), G931 (= G238), V960 (≠ G267), Y961 (= Y268), S962 (≠ M269), N963 (= N270), T964 (= T271)

Sites not aligning to the query:

binding iron/sulfur cluster: 458, 657, 659, 664, 665, 666, 667, 668, 670, 674, 675, 679, 708, 715, 716, 717, 718, 719, 721, 725, 726, 731, 732, 781, 782, 785, 787, 810
binding thiamine diphosphate: 27, 28, 29, 63, 787, 808, 809, 810, 839

2c3uA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus, oxygen inhibited form (see paper)
32% identity, 32% coverage: 231:380/469 of query aligns to 956:1100/1231 of 2c3uA

query
sites
2c3uA

V

V

M

W

A

I

L

F

A

G

G
|
G

D
|
D

G
|
G

G
x
W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V

V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E

E

G
x
V

Y
|
Y

M
x
S

N
|
N

T
|
T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A
|
A

A
x
E

S

S

F
|
F

E

P

G
|
G

F

P

S
|
S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P
x
I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

active site: N995 (= N270)
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: G961 (= G236), D962 (= D237), G963 (= G238), W964 (≠ G239), V992 (≠ G267), Y993 (= Y268), S994 (≠ M269), N995 (= N270), T996 (= T271)
binding calcium ion: D982 (≠ K257), N984 (≠ L259), A1055 (= A336), E1056 (≠ A337), F1058 (= F339), G1060 (= G341), S1062 (= S343)
binding magnesium ion: D962 (= D237), T990 (≠ N265), V992 (≠ G267)
binding pyruvic acid: N995 (= N270)
binding iron/sulfur cluster: C1070 (= C351), I1071 (≠ P352)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: 28, 29, 63, 87, 816, 837, 839, 868, 869
binding pyruvic acid: 30, 113, 122
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 811, 814, 816, 839

2c3pA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
32% identity, 32% coverage: 231:380/469 of query aligns to 956:1100/1231 of 2c3pA

query
sites
2c3pA

V

V

M

W

A

I

L

F

A

G

G
|
G

D
|
D

G
|
G

G

W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V
|
V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E

E

G
x
V

Y
|
Y

M
x
S

N
|
N

T
|
T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A
|
A

A
x
E

S

S

F
|
F

E

P

G
|
G

F

P

S
|
S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P
x
I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

active site: N995 (= N270)
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: G961 (= G236), D962 (= D237), G963 (= G238), V992 (≠ G267), Y993 (= Y268), S994 (≠ M269), N995 (= N270), T996 (= T271)
binding calcium ion: D982 (≠ K257), V983 (= V258), N984 (≠ L259), A1055 (= A336), E1056 (≠ A337), F1058 (= F339), G1060 (= G341), S1062 (= S343)
binding magnesium ion: D962 (= D237), T990 (≠ N265), V992 (≠ G267)
binding iron/sulfur cluster: S994 (≠ M269), C1070 (= C351), I1071 (≠ P352)

Sites not aligning to the query:

active site: 30, 63, 113
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: 28, 29, 30, 63, 87, 113, 816, 837, 838, 839, 868, 869
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

2c3oA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
32% identity, 32% coverage: 231:380/469 of query aligns to 956:1100/1231 of 2c3oA

query
sites
2c3oA

V

V

M

W

A

I

L

F

A

G

G
|
G

D
|
D

G
|
G

G

W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V

V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E

E

G
x
V

Y
|
Y

M
x
S

N
|
N

T
|
T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A

A

A
x
E

S

S

F
|
F

E

P

G
|
G

F

P

S

S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P
x
I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

active site: N995 (= N270)
binding calcium ion: D982 (≠ K257), N984 (≠ L259), E1056 (≠ A337), F1058 (= F339), G1060 (= G341)
binding magnesium ion: D962 (= D237), T990 (≠ N265), V992 (≠ G267)
binding pyruvic acid: T996 (= T271)
binding iron/sulfur cluster: C1070 (= C351), I1071 (≠ P352)
binding thiamine diphosphate: G961 (= G236), D962 (= D237), G963 (= G238), V992 (≠ G267), Y993 (= Y268), S994 (≠ M269), N995 (= N270), T996 (= T271)

Sites not aligning to the query:

active site: 30, 63, 113
binding pyruvic acid: 30, 113
binding iron/sulfur cluster: 458, 681, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 746, 747, 748, 749, 750, 754, 760, 761, 810, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 87, 816, 837, 838, 839, 868

1kekA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase (see paper)
32% identity, 32% coverage: 231:380/469 of query aligns to 956:1100/1231 of 1kekA

query
sites
1kekA

V

V

M

W

A

I

L

F

A

G

G
|
G

D
|
D

G
|
G

G

W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V

V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E

E

G
x
V

Y
|
Y

M
x
S

N
|
N

T
|
T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A
|
A

A
x
E

S

S

F
|
F

E

P

G

G

F

P

S
|
S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P
x
I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

active site: N995 (= N270)
binding calcium ion: D982 (≠ K257), N984 (≠ L259), A1055 (= A336), E1056 (≠ A337), F1058 (= F339), S1062 (= S343)
binding 2-acetyl-thiamine diphosphate: G961 (= G236), D962 (= D237), G963 (= G238), V992 (≠ G267), Y993 (= Y268), S994 (≠ M269), N995 (= N270), T996 (= T271)
binding magnesium ion: D962 (= D237), T990 (≠ N265), V992 (≠ G267)
binding iron/sulfur cluster: C1070 (= C351), I1071 (≠ P352)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-acetyl-thiamine diphosphate: 29, 63, 87, 113, 816, 837, 838, 839, 868
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 703, 737, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

1b0pA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
32% identity, 32% coverage: 231:380/469 of query aligns to 956:1100/1231 of 1b0pA

query
sites
1b0pA

V

V

M

W

A

I

L

F

A

G

G
|
G

D
|
D

G
|
G

G

W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V

V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E

E

G
x
V

Y
|
Y

M
x
S

N
|
N

T
|
T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A
|
A

A
x
E

S

S

F
|
F

E

P

G
|
G

F

P

S

S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P
x
I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

active site: N995 (= N270)
binding calcium ion: D982 (≠ K257), N984 (≠ L259), A1055 (= A336), E1056 (≠ A337), F1058 (= F339), G1060 (= G341)
binding magnesium ion: D962 (= D237), T990 (≠ N265), V992 (≠ G267)
binding iron/sulfur cluster: S994 (≠ M269), C1070 (= C351), I1071 (≠ P352)
binding thiamine diphosphate: G961 (= G236), D962 (= D237), G963 (= G238), V992 (≠ G267), Y993 (= Y268), S994 (≠ M269), N995 (= N270), T996 (= T271)

Sites not aligning to the query:

active site: 30, 63, 113
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 737, 744, 745, 747, 748, 750, 754, 755, 760, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 816, 837, 838, 839, 868

P94692 Pyruvate:ferredoxin oxidoreductase; PFOR; POR; Pyruvate synthase; EC 1.2.7.1 from Desulfocurvibacter africanus (Desulfovibrio africanus) (see 3 papers)
30% identity, 36% coverage: 213:380/469 of query aligns to 941:1101/1232 of P94692

query
sites
P94692

M

L

L

L

S

G

G

Q

I

I

R

A

Y

M

Y

S

D

D

R

L

I

Y

G

T

R

K

K

K

D

S

I

V

V

W

V

I

M

-

A

-

L

F

A

G

G
|
G

D
|
D

G
|
G

G
x
W

T

A

A

Y

D

D

C

I

G

G

F

Y

H

G

A

G

V

L

S

D

G

H

A

V

A

L

E

A

R

S

G

G

E

E

K
x
D

V

V

L
x
N

Y

V

V

F

C

V

V

M

D

D

N
x
T

E
|
E

G
x
V

Y
|
Y

M
x
S

N
|
N

T

T

G

G

M

G

Q

Q

R

S

S

S

G

K

T

A

T

T

P

P

Y

T

G

G

S

A

W

V

T

A

S

K

T

F

T

A

P

A

V

A

G

-

E

-

H

-

M

-

Q

-

G

G

K

K

T

R

Q

T

D

G

A

K

K

K

N

D

L

L

P

A

L

R

I

M

M

V

T

M

A

T

H

Y

K

G

C

Y

R

V

Y

Y

V

V

A

A

T

T

A

V

S

S

T

M

G

G

Y

Y

M

S

E

K

D

Q

L

Q

Y

F

E

L

K

K

L

V

D

L

R

K

A

E

I

-

A
|
A

A

E

S

S

F
|
F

E

P

G
|
G

F

P

S
|
S

Y

L

L

V

H

I

I

A

Y

Y

S

A

P

T

C
|
C

P

I

T

N

-

Q

G

G

W

L

R

R

I

K

P

G

S

M

S

G

K

K

V

S

I

Q

D

D

A

V

C

M

R

N

L

T

S

A

V

V

D

K

S

S

N

G

F

Y

N

W

P

P

L

L

W

F

E

R

Y

Y

GDGW 962:965 (≠ GDGG 236:239) binding
D963 (= D237) binding
D983 (≠ K257) binding
N985 (≠ L259) binding
T991 (≠ N265) binding
TEVYSN 991:996 (≠ NEGYMN 265:270) binding
V993 (≠ G267) binding
A1056 (= A336) binding
F1059 (= F339) binding
G1061 (= G341) binding
S1063 (= S343) binding
C1071 (= C351) binding

Sites not aligning to the query:

31 binding
64 binding
114 binding
689 binding
692 binding
695 binding
699 binding
745 binding
748 binding
751 binding
755 binding
812 binding
815 binding
817 binding
840 binding ; binding

7qv7A Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
29% identity, 29% coverage: 15:152/469 of query aligns to 12:169/174 of 7qv7A

query
sites
7qv7A

C
|
C

D
x
L

G
|
G

C
|
C

G
x
Y

D

T

C
|
C

M

I

T

A

A

A

C
|
C

S

A

-

F

-

V

-
x
H

Q

E

A

E

K

Q

G

G

G

L

A

Q

S

P

R

F

I

P

S

R

I
x
L

V

Y

P

L

G

T

A

Y

K

T

A

S

G

E

S

G

F

I

E

M

T

P

A

I

L

Q

C
|
C

R
|
R

Q

H

C
|
C

G

E

D

D

P

A

K

P

C
|
C

V

A

Q

E

V

V

C
|
C

P

P

S

V

G

E

A

A

L

I

D

K

K

K

D

E

G

G

G

N

N

A

G

I

I

I

G

-

W

-

D

D

A

E

S

K

K

A

C
|
C

V
x
I

D
x
G

C
|
C

L

K

L

T

C
|
C

T

L

A

L

G

A

C
|
C

A

S

Y

F

G

G

G

A

I
|
I

-

D

-
x
F

-

S

-

V

-

Q

-

D

-

S

-

L

-

E

-

Q

-

S

-

I

-

F

-

K

A

D

V

I

D

K

E

E

S

N

V

L

A

M

R

R

V

I

-

V

-

A

G

V

K

K

C
|
C

D

D

Q
x
L

C
|
C

-

N

-

F

-

R

D

E

G

E

D

G

P

P

A
|
A

C
|
C

V

V

K

Q

V

F

C
|
C

P

P

T

T

G

K

A

A

L

L

E

K

F

L

R

V

K

D

A

G

G

D

S

E

I

I

F

N

N

K

E

M

Y

V

G

K

A

N

K

K

E

R

D

T

L

V

F

N

V

V

P

E

G

S

L

L

binding iron/sulfur cluster: C12 (= C15), L13 (≠ D16), G14 (= G17), C15 (= C18), Y16 (≠ G19), C18 (= C21), C22 (= C25), H26 (vs. gap), L37 (≠ I37), C51 (= C51), R52 (= R52), C54 (= C54), C59 (= C59), C63 (= C63), C82 (= C84), I83 (≠ V85), G84 (≠ D86), C85 (= C87), C88 (= C90), C92 (= C94), I97 (= I99), F99 (vs. gap), C125 (= C111), L127 (≠ Q113), C128 (= C114), A136 (= A119), C137 (= C120), C141 (= C124)

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
27% identity, 49% coverage: 155:386/469 of query aligns to 25:236/291 of 6n2oD

query
sites
6n2oD

C
|
C

Q

P

G

G

C
|
C

N

G

S

H

E

F

L

G

I

I

M

L

R

N

H

G

A

V

M

Y

R

R

K

A

I

M

G

A

R

E

N

L

S

G

V

I

L

D

A

S

T

T

P

K

P

F

G

A

C

A

I

I

P

S

G

G

M
x
I

G
|
G

S
x
C

V

-

G

-

Y

-

N

-

G

-

K

-

T

-

G
x
S

T

S

K
x
R

V

M

P

P

V

Y

F

F

-

V

-

D

-

S

-

Y

-

K

-

M

H

H

P

T

L

L

L
x
H

T

G

N

R

T

A

A

G

S

A

M

V

L

A

S

T

G

G

I

T

R

Q

R

-

Y

-

D

-

R

-

I

V

G

A

R

R

K

P

D

D

I

L

V

C

V

V

M

V

A

V

L

A

A

G

G

G

D
|
D

G
|
G

G
x
D

T

G

A

F

D

S

C

I

G

G

F

G

H

G

A

H

V

M

S

P

G

H

A

M

A

A

E

R

R

K

G

N

E

V

K

N

V

M

L

T

Y

Y

V

V

C

L

V

M

D

D

N
|
N

E

G

G
x
I

Y

Y

M
x
G

N
x
L

T
|
T

G
x
K

M

G

Q

Q

R

Y

S

S

G

P

T

T

T

S

P

R

Y

P

G

E

S

M

W

T

T

A

S

Y

T

T

P

P

V

Y

G

-

E

-

H

-

M

-

Q

-

G

G

K

G

T

P

Q

E

D

N

A

P

K

M

N

N

L

P

P

L

L

L

I

Y

M

M

T

L

A

T

H

Y

K

G

C

A

R

T

Y

Y

V

V

A

A

T

Q

A

A

S

F

T

A

G

G

Y

K

M

P

E

K

D

D

L

C

Y

A

E

E

K

L

L

I

D

K

R

G

A

A

I

M

A

-

S

E

F

H

E

E

G

G

F

F

S

A

Y

Y

L

V

H

N

I

I

Y

F

S

S

P

Q

C
|
C

P

P

T
|
T

G
x
F

W

N

R

K

I

I

P

-

S

-

K

D

V

T

I

V

D

D

A

F

C

Y

R

R

L

D

S

L

V

V

D

E

S

-

N

-

F

-

N

-

P

P

L

I

W

P

E

E

Y

D

T

H

N

D

T

T

S

S

G

D

L

L

binding magnesium ion: D101 (= D237), N129 (= N265), I131 (≠ G267)
binding succinyl-coenzyme a: I57 (≠ M187), R62 (≠ K199), L134 (≠ N270), K136 (≠ G272)
binding iron/sulfur cluster: C25 (= C155), C28 (= C158), C59 (≠ S189), C208 (= C351), T210 (= T353), F211 (≠ G354)
binding thiamine diphosphate: I57 (≠ M187), G58 (= G188), C59 (≠ S189), S60 (≠ G197), H76 (≠ L207), G102 (= G238), D103 (≠ G239), N129 (= N265), I131 (≠ G267), G133 (≠ M269), L134 (≠ N270), T135 (= T271)

Sites not aligning to the query:

binding iron/sulfur cluster: 24

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
27% identity, 49% coverage: 155:386/469 of query aligns to 25:236/291 of 6n2oB

query
sites
6n2oB

C
|
C

Q

P

G

G

C
|
C

N

G

S

H

E

F

L

G

I

I

M

L

R

N

H

G

A

V

M

Y

R

R

K

A

I

M

G

A

R

E

N

L

S

G

V

I

L

D

A

S

T

T

P

K

P

F

G

A

C

A

I

I

P

S

G

G

M
x
I

G
|
G

S
x
C

V

-

G

-

Y

-

N

-

G

-

K

-

T

-

G
x
S

T

S

K
x
R

V

M

P

P

V

Y

F

F

-

V

-

D

-

S

-

Y

-

K

-

M

H

H

P

T

L

L

L
x
H

T

G

N

R

T

A

A

G

S

A

M

V

L

A

S

T

G

G

I

T

R

Q

R

-

Y

-

D

-

R

-

I

V

G

A

R

R

K

P

D

D

I

L

V

C

V

V

M

V

A

V

L

A

A

G

G

G

D
|
D

G
|
G

G
x
D

T

G

A

F

D

S

C

I

G

G

F

G

H

G

A

H

V

M

S

P

G

H

A

M

A

A

E

R

R

K

G

N

E

V

K

N

V

M

L

T

Y

Y

V

V

C

L

V

M

D

D

N
|
N

E

G

G
x
I

Y
|
Y

M
x
G

N
x
L

T
|
T

G
x
K

M

G

Q

Q

R

Y

S

S

G

P

T

T

T

S

P

R

Y

P

G

E

S

M

W

T

T

A

S
x
Y

T

T

P

P

V

Y

G

-

E

-

H

-

M

-

Q

-

G

G

K

G

T

P

Q

E

D

N

A

P

K

M

N

N

L

P

P

L

L

L

I

Y

M

M

T

L

A

T

H

Y

K

G

C

A

R

T

Y

Y

V

V

A

A

T

Q

A

A

S

F

T

A

G

G

Y

K

M

P

E

K

D

D

L

C

Y

A

E

E

K

L

L

I

D

K

R

G

A

A

I

M

A

-

S

E

F

H

E

E

G

G

F

F

S

A

Y

Y

L

V

H

N

I

I

Y

F

S

S

P

Q

C
|
C

P

P

T
|
T

G
x
F

W

N

R

K

I

I

P

-

S

-

K

D

V

T

I

V

D

D

A

F

C

Y

R

R

L

D

S

L

V

V

D

E

S

-

N

-

F

-

N

-

P

P

L

I

W

P

E

E

Y

D

T

H

N

D

T

T

S

S

G

D

L

L

binding 2-oxoglutaric acid: R62 (≠ K199), L134 (≠ N270)
binding coenzyme a: K136 (≠ G272), Y150 (≠ S286)
binding magnesium ion: D101 (= D237), N129 (= N265), I131 (≠ G267)
binding iron/sulfur cluster: C25 (= C155), C28 (= C158), C59 (≠ S189), C208 (= C351), T210 (= T353), F211 (≠ G354)
binding thiamine diphosphate: I57 (≠ M187), G58 (= G188), C59 (≠ S189), S60 (≠ G197), H76 (≠ L207), G102 (= G238), D103 (≠ G239), N129 (= N265), I131 (≠ G267), Y132 (= Y268), G133 (≠ M269), L134 (≠ N270), T135 (= T271)

Sites not aligning to the query:

binding iron/sulfur cluster: 24

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
27% identity, 49% coverage: 155:386/469 of query aligns to 25:236/291 of 6n2nB

query
sites
6n2nB

C
|
C

Q

P

G

G

C
|
C

N

G

S
x
H

E

F

L

G

I

I

M

L

R

N

H

G

A

V

M

Y

R

R

K

A

I

M

G

A

R

E

N

L

S

G

V

I

L

D

A

S

T

T

P

K

P

F

G

A

C

A

I

I

P

S

G

G

M
x
I

G
|
G

S
x
C

V

-

G

-

Y

-

N

-

G

-

K

-

T

-

G
x
S

T

S

K

R

V

M

P

P

V

Y

F

F

-

V

-

D

-

S

-

Y

-

K

-

M

H

H

P

T

L

L

L
x
H

T

G

N

R

T

A

A

G

S

A

M

V

L

A

S

T

G

G

I

T

R

Q

R

-

Y

-

D

-

R

-

I

V

G

A

R

R

K

P

D

D

I

L

V

C

V

V

M

V

A

V

L

A

A

G

G
|
G

D
|
D

G
|
G

G
x
D

T

G

A

F

D

S

C

I

G

G

F

G

H

G

A

H

V

M

S

P

G

H

A

M

A

A

E

R

R

K

G

N

E

V

K

N

V

M

L

T

Y

Y

V

V

C

L

V

M

D

D

N
|
N

E

G

G
x
I

Y
|
Y

M
x
G

N
x
L

T
|
T

G

K

M

G

Q

Q

R

Y

S

S

G

P

T

T

T

S

P

R

Y

P

G

E

S

M

W

T

T

A

S

Y

T

T

P

P

V

Y

G

-

E

-

H

-

M

-

Q

-

G

G

K

G

T

P

Q

E

D

N

A

P

K

M

N

N

L

P

P

L

L

L

I

Y

M

M

T

L

A

T

H

Y

K

G

C

A

R

T

Y

Y

V

V

A

A

T

Q

A

A

S

F

T

A

G

G

Y

K

M

P

E

K

D

D

L

C

Y

A

E

E

K

L

L

I

D

K

R

G

A

A

I

M

A

-

S

E

F

H

E

E

G

G

F

F

S

A

Y

Y

L

V

H

N

I

I

Y

F

S

S

P

Q

C
|
C

P

P

T
|
T

G
x
F

W

N

R

K

I

I

P

-

S

-

K

D

V

T

I

V

D

D

A

F

C

Y

R

R

L

D

S

L

V

V

D

E

S

-

N

-

F

-

N

-

P

P

L

I

W

P

E

E

Y

D

T

H

N

D

T

T

S

S

G

D

L

L

binding magnesium ion: D101 (= D237), N129 (= N265), I131 (≠ G267)
binding iron/sulfur cluster: C25 (= C155), C28 (= C158), H30 (≠ S160), C59 (≠ S189), C208 (= C351), T210 (= T353), F211 (≠ G354)
binding thiamine diphosphate: I57 (≠ M187), G58 (= G188), C59 (≠ S189), S60 (≠ G197), H76 (≠ L207), G100 (= G236), D101 (= D237), G102 (= G238), D103 (≠ G239), N129 (= N265), I131 (≠ G267), Y132 (= Y268), G133 (≠ M269), L134 (≠ N270), T135 (= T271)

Sites not aligning to the query:

binding iron/sulfur cluster: 24

7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
31% identity, 25% coverage: 15:131/469 of query aligns to 14:161/183 of 7qv7B

query
sites
7qv7B

C
|
C

D

I

G
|
G

C
|
C

G
x
K

D
x
A

C
|
C

M

E

T

V

A

A

C
|
C

S

F

Q

A

-

V

-
x
H

-

N

-

R

-

N

-

H

-

V

-

G

A

A

K

T

G

V

G

G

A

T

S

V

R

S

I

I

S

P

I

V

V

I

P

P

G
x
R

A

L

K

H

A

L

G

I

S

K

F

T

E

E

T

H

A

G

L

T

-

M

-

P

-

I

-

Q

C
|
C

R
|
R

Q
x
H

C
|
C

G

E

D

D

P
x
A

K

P

C
|
C

V

A

Q

N

V

V

C
|
C

P

T

S

V

G

G

A
|
A

L
x
I

D

K

K

R

D

E

G

-

G

G

N

N

G

A

I

I

I

V

G

-

W

V

D

D

A

E

S

K

K

L

C
|
C

V
x
I

D

G

C
|
C

L

K

L

S

C
|
C

T

L

A

L

G

A

C
|
C

A

P

Y

F

G

G

G

A

I
|
I

A

E

-

L

-

P

-

Q

-

Y

-

E

-

D

-

G

-

R

-

E

-

V

-

F

-

Q

-

I

-

N

-

L

-

K

-

L

V

V

D

Q

E

E

S

P

V

R

A

I

R

I

V

A

G

Y

K

K

C
|
C

D

D

Q
x
L

C
|
C

D

N

-

D

-

L

G

G

D

E

P
|
P

A

A

C
|
C

V

V

K

K

V

A

C
|
C

P
|
P

T

E

G

N

A

A

L
|
L

E

T

F

L

binding iron/sulfur cluster: C14 (= C15), G16 (= G17), C17 (= C18), K18 (≠ G19), A19 (≠ D20), C20 (= C21), C24 (= C25), H28 (vs. gap), R48 (≠ G40), C63 (= C51), R64 (= R52), H65 (≠ Q53), C66 (= C54), A69 (≠ P57), C71 (= C59), C75 (= C63), A79 (= A67), I80 (≠ L68), C94 (= C84), I95 (≠ V85), C97 (= C87), C100 (= C90), C104 (= C94), I109 (= I99), C139 (= C111), L141 (≠ Q113), C142 (= C114), P148 (= P118), C150 (= C120), C154 (= C124), P155 (= P125), L159 (= L129)

2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
30% identity, 27% coverage: 7:131/469 of query aligns to 5:142/193 of 2vpyF

query
sites
2vpyF

S

A

I

M

S

A

F

I

K

D

A

L

S

S

L

L

C
|
C

D

V

G
|
G

C
|
C

G

A

D

A

C
|
C

M

A

T

V

A

A

C
|
C

-

K

-

M

-

E

S
x
N

Q

E

A

V

K

P

G

P

G

G

A

V

S

F

R

N

I
x
L

S

W

I
|
I

-

R

-

E

-

R

-

E

-

V

-

G

-

E

V

Y

P

P

G

N

A

L

K

V

A

V

G

-

S

E

F

F

E

R

T
x
P

A

E

L
x
Q

C
|
C

R
x
L

Q

H

C
|
C

G

E

D

N

P

P

K
x
P

C
|
C

V

V

Q

P

V

V

C
|
C

P
|
P

S

T

G

G

A

A

L

-

D

S

K

Y

D

Q

G

T

G

K

N

D

G

G

I

L

I
x
V

G

L

W

V

D

D

A

P

S

K

K

K

C
|
C

V
x
I

D
x
A

C
|
C

L
x
G

L

A

C
|
C

T

I

A

A

G

A

C
|
C

A
x
P

Y
|
Y

G

D

G

A

I
x
R

A

Y

V

L

D

H

E

P

S

A

V

-

A

G

R

Y

V
|
V

G

S

K

K

C
|
C

D

T

Q
x
F

C
|
C

D

A

G

H

D

R

-

L

-

E

-

K

-

G

-

K

-

V

P
|
P

A
|
A

C
|
C

V

V

K

E

V

T

C
|
C

P

P

T

T

G

Y

A
x
C

L
x
R

E

T

F

F

binding pentachlorophenol: I91 (≠ V85), C93 (= C87)
binding iron/sulfur cluster: C13 (= C15), G15 (= G17), C16 (= C18), C19 (= C21), C23 (= C25), N27 (≠ S26), L36 (≠ I35), I38 (= I37), P55 (≠ T48), Q57 (≠ L50), C58 (= C51), L59 (≠ R52), C61 (= C54), P65 (≠ K58), C66 (= C59), C70 (= C63), P71 (= P64), V83 (≠ I77), C90 (= C84), I91 (≠ V85), A92 (≠ D86), C93 (= C87), G94 (≠ L88), C96 (= C90), C100 (= C94), P101 (≠ A95), Y102 (= Y96), R105 (≠ I99), V113 (= V108), C116 (= C111), F118 (≠ Q113), C119 (= C114), P129 (= P118), A130 (= A119), C131 (= C120), C135 (= C124), C139 (≠ A128), R140 (≠ L129)

Sites not aligning to the query:

binding iron/sulfur cluster: 189, 190

Query Sequence

>WP_011384089.1 NCBI__GCF_000009985.1:WP_011384089.1
MGVAFDSISFKASLCDGCGDCMTACSQAKGGASRISIVPGAKAGSFETALCRQCGDPKCV
QVCPSGALDKDGGNGIIGWDASKCVDCLLCTAGCAYGGIAVDESVARVGKCDQCDGDPAC
VKVCPTGALEFRKAGSIFNEYGAKEDLFVPGLSGCQGCNSELIMRHAMRKIGRNSVLATP
PGCIPGMGSVGYNGKTGTKVPVFHPLLTNTASMLSGIRRYYDRIGRKDIVVMALAGDGGT
ADCGFHAVSGAAERGEKVLYVCVDNEGYMNTGMQRSGTTPYGSWTSTTPVGEHMQGKTQD
AKNLPLIMTAHKCRYVATASTGYMEDLYEKLDRAIAASFEGFSYLHIYSPCPTGWRIPSS
KVIDACRLSVDSNFNPLWEYTNTSGLRFTHSPDNAHPVSAYVKMIGKYRHLSPDQLAHIQ
AKVDEQVALLKLMAAESPPPPAAHRAPAGSSTPAGAEPSQPARREIARN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory